Publications by authors named "Kanako Masuda"

12 Publications

  • Page 1 of 1

Effects on cervical vestibular-evoked myogenic potentials of four clinically used head and neck measurement positions in healthy subjects.

Acta Otolaryngol 2021 Aug 27;141(8):729-735. Epub 2021 Jul 27.

Division of Hearing and Balance Research, National Institute of Sensory Organs, National Hospital Organization Tokyo Medical Center, Tokyo, Japan.

Background: The most reliable head and neck position for cervical vestibular-evoked myogenic potentials (cVEMPs) measurements yet to be determined.

Aims/objectives: To assess how four body positions used during clinical recordings of cVEMPs affect cVEMP parameters.

Material And Method: cVEMPs of 10 healthy subjects (26-50 years old) were recorded in four body positions: sitting/head rotated; supine/head rotated; semi-recumbent/head rotated and elevated; supine/head elevated.

Results: Mean background sternocleidomastoid muscle (SCM) electrical activity was significantly higher in positions C and D than in positions A and B. The latencies of p13 and n23 differed significantly among the four positions. Raw p13-n23 complex amplitude was significantly greater in positions C and D than in A and B. These differences were reduced when amplitudes were corrected by SCM activity. For positions A and B, one and two subjects, respectively, had an abnormal raw asymmetry ratio (AR). After correction, all subjects had normal ARs in all positions.

Conclusions And Significance: Body positions in which the head is elevated produce a quicker and larger cVEMP response compared to positions in which the head is not elevated. The difference in ARs among positions can be ignored as long as the correction is made.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/00016489.2021.1943520DOI Listing
August 2021

Subtype screening of bla genes using bipartite primers for DNA sequencing.

Jpn J Infect Dis 2021 Mar 31. Epub 2021 Mar 31.

Department of Microbiology, Ehime Prefectural Institute of Public Health and Environmental Science, Japan.

Genes conferring carbapenem resistance have spread worldwide among gram-negative bacteria. Subtyping of these genes has epidemiological value due to the global cross-border movement of people. Subtyping of bla genes that frequently detected in Japan appears to be important in public health settings; however, there are few useful tools for this purpose. We developed a subtyping screening tool based on PCR direct sequencing, which targets the internal sequences of almost all bla genes. The tool used bipartite multiplex primers with M13 universal sequences at the 5'-end. According to in silico analysis, among the 78 known IMP-type genes, except for bla, 77 detected genes were estimated to be differentiated. In vitro evaluation indicated that sequences of amplicons of IMP-1, IMP-6, IMP-7, and IMP-20 templates were identical to their respective subtypes. Even if the amplicons were small or undetectable through the first PCR, sufficient amplicons for DNA sequencing were obtained through a second PCR using the M13 universal primers. In conclusion, our tool can be possibly used for subtype screening of bla, which is useful for the surveillance of bacteria with bla in clinical and public health settings or environmental fields.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7883/yoken.JJID.2020.926DOI Listing
March 2021

Implanted Port Catheter System Infection Caused by Methicillin-resistant Staphylococcus pseudintermedius ST71-SCCmec type III.

Intern Med 2021 Jul 15;60(14):2337-2340. Epub 2021 Feb 15.

Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Japan.

Staphylococcus pseudintermedius is commonly associated with skin and soft tissue infections in dogs. However, infections caused by S. pseudintermedius are only rarely reported in humans, and this pathogen is frequently misidentified as S. aureus. We herein report a case of an implanted port catheter system infection caused by methicillin-resistant S. pseudintermedius (MRSP) in a patient with hepatocellular carcinoma. The patient was also a dog owner. S. pseudintermedius was first identified using the Vitek2 system (BioMérieux). Whole-genome sequencing revealed that this MRSP was a sequence type 71-carrying staphylococcal cassette chromosome mec type III (ST71-SCCmec III) isolate.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2169/internalmedicine.5579-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355384PMC
July 2021

Single-Tube Multiplex Polymerase Chain Reaction for the Detection of Genes Encoding Enterobacteriaceae Carbapenemase.

Jpn J Infect Dis 2020 Mar 29;73(2):166-172. Epub 2019 Nov 29.

Department of Microbiology, Ehime Prefectural Institute of Public Health and Environmental Science.

A multiplex PCR assay in a single tube was developed for the detection of the carbapenemase genes of Enterobacteriaceae. Primers were designed to amplify the following six carbapenemase genes: bla, bla, bla, bla, bla, and bla. Of 70 bla variants, 67 subtypes were simulated to be PCR-positive based on in silico simulation and the primer-design strategy. After determining the optimal PCR conditions and performing in vitro assays, the performance of the PCR assay was evaluated using 51 and 91 clinical isolates with and without carbapenemase genes, respectively. In conclusion, the combination of multiplex PCR primers and QIAGEN Multiplex PCR Plus Kit was used to determine the best performance for the rapid and efficient screening of carbapenemase genes in Enterobacteriaceae. The assay had an overall sensitivity and specificity of 100%. This PCR assay compensates for the limitations of phenotypic testing, such as antimicrobial susceptibility testing and the modified carbapenem inactivation method, in clinical and public health settings.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7883/yoken.JJID.2019.041DOI Listing
March 2020

Staphylococcus aureus Isolated from Skin from Atopic-Dermatitis Patients Produces Staphylococcal Enterotoxin Y, Which Predominantly Induces T-Cell Receptor Vα-Specific Expansion of T Cells.

Infect Immun 2020 01 22;88(2). Epub 2020 Jan 22.

Department of Bacteriology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan

While investigating the virulence traits of adhering to the skin of atopic-dermatitis (AD) patients, we identified a novel open reading frame (ORF) with structural similarity to a superantigen from genome sequence data of an isolate from AD skin. Concurrently, the same ORF was identified in a bovine isolate of and designated SElY (H. K. Ono, Y. Sato'o, K. Narita, I. Naito, et al., Appl Environ Microbiol 81:7034-7040, 2015, https://doi.org/10.1128/AEM.01873-15). Recombinant SElY had superantigen activity in human peripheral blood mononuclear cells. It further demonstrated emetic activity in a primate animal model, and it was proposed that SElY be renamed SEY (H. K. Ono, S. Hirose, K. Narita, M. Sugiyama, et al., PLoS Pathog 15:e1007803, 2019, https://doi.org/10.1371/journal.ppat.1007803). Here, we investigated the prevalence of the gene in 270 human clinical isolates of various origins in Japan. Forty-two strains were positive for the gene, and the positive isolates were from patients with the skin diseases atopic dermatitis and impetigo/staphylococcal scalded skin syndrome (SSSS), with a detection rate of ∼17 to 22%. There were three variants of SEY (SEY, SEY, and SEY), and isolates producing SEY variants formed three distinct clusters corresponding to clonal complexes (CCs) 121, 59, and 20, respectively. Most isolates produced SEY in broth culture. Unlike SEY, the three recombinant SEY variants exhibited stability against heat treatment. SEY predominantly activated human T cells with a particular T-cell receptor (TCR) Vα profile, a unique observation since most staphylococcal enterotoxins exert their superantigenic activities through activating T cells with specific TCR Vβ profiles. SEY may act to induce localized inflammation via skin-resident T-cell activation, facilitating the pathogenesis of infection in disrupted epithelial barriers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1128/IAI.00360-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6977126PMC
January 2020

Epidemiological Aspects of Outbreaks in Japan and Genetic Characteristics of the Causative Pathogen.

Foodborne Pathog Dis 2020 02 11;17(2):144-150. Epub 2019 Oct 11.

Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo, Japan.

Zoonotic pathogen has been identified as the cause of several human disease outbreaks; however, factors such as the general symptoms and incubation period of infection have yet to be defined. Therefore, we aimed to determine the unique aspects of outbreaks in Japan and to examine the genetic characteristics of the causative pathogen. We studied all known outbreaks that occurred in Japan up until 2015, which consisted of five confirmed outbreaks and one putative outbreak (Outbreaks 1-6). Outbreaks were re-examined based on personal communications between researchers in prefectural and municipal public health institutes, and through examination of any published study conducted at the time. Draft genome sequences of outbreak-associated isolates were also generated. The most common symptom displayed by patients across the six episodes was watery diarrhea (>80%), followed by abdominal pain (50-84%) and fever (37.0-39.5°C) (26-44%). The estimated average incubation period of infection was 12-24 h. We assumed that most of the outbreaks were foodborne or waterborne, with restaurant foods, restaurant water, and boxed lunches being the suspected transmission vehicles. Three of the six outbreak-associated isolates possessed intact ETT2 regions, while the remaining isolates contained disrupted ETT2-encoding genes. Virulence gene screening revealed that more than half (44/70) of the tested genes were present in all 5 strains examined, and that each of the strains contained more than 1 gene from 14 out of the 21 groups of virulence genes examined in this study. The five strains were classified into four of the five known phylogroups. Therefore, we determined that multiple genotypes in Japan have the potential to cause outbreaks of diarrhea, abdominal pain, and/or fever following infection of a human host.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/fpd.2019.2654DOI Listing
February 2020

Low incidence of Staphylococcus argenteus bacteremia in Hiroshima, Japan.

J Infect Chemother 2020 Jan 31;26(1):140-143. Epub 2019 Jul 31.

Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima 734-8551, Japan; Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo 189-0002, Japan; Department of Antimicrobial Resistance, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8551, Japan.

Staphylococcus argenteus is a globally distributed cause of human infection; however, it has been misidentified as Staphylococcus aureus in diagnostic laboratories. Invasive infection caused by S. argenteus has been increasingly reported worldwide. However, there have been no reports on S. argenteus bacteremia in Japan. Therefore, we conducted a retrospective study to investigate the incidence of S. argenteus bacteremia at Hiroshima University Hospital between 2013 and 2017. S. argenteus was identified based on the absence of the crtM gene and multi-locus sequence typing. S. argenteus was identified in 2 of the 201 S. aureus blood culture isolates (1.0%). Both S. argenteus isolates belonged to sequence type 2250 harboring the staphylococcal enterotoxin Y gene (sey) and were susceptible to methicillin. One of them was penicillin-resistant, harboring a blaZ gene. The primary sites of infection were lower leg cellulitis and catheter-related blood stream infection. No patients died during hospitalization. This study suggested a low incidence of S. argenteus bacteremia in Japan when compared with reports from other countries. Further studies are necessary to investigate the prevalence of S. argenteus infection in Japan and the clinical impact of S. argenteus compared to S. aureus.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jiac.2019.07.011DOI Listing
January 2020

Psychiatric disorders in patients with intractable dizziness in the department of otolaryngology.

Acta Otolaryngol 2018 Jul 31;138(7):646-647. Epub 2018 Jan 31.

a Department of Otolaryngology , National Hospital Organization Tokyo Medical Center , Tokyo , Japan.

Objective: Vertigo and dizziness are often not fully explained by organic illnesses, but instead may be related to psychiatric disorders. It is important to determine the types of psychiatric comorbidities that are frequent in cases of intractable dizziness.

Method: The study subjects were 90 patients who had experienced intractable dizziness for more than three months and were referred to a psychiatrist when their symptoms could not be fully explained based on their physical illness. The patients' final diagnosis and questionnaire (DHI,SDS and STAI) scores were evaluated.

Results: Seventy percent (63/90) of patients with intractable dizziness had been diagnosed as having psychiatric conditions by the study psychiatrists. The most common diagnosis was unspecified depressive disorder.

Conclusions: Psychiatric comorbidities seem to be more prevalent in certain subgroups of organic dizziness. We found a higher rate of depressive disorders in the sequelae of sudden deafness and migraine-related dizziness.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/00016489.2018.1429652DOI Listing
July 2018

Autotaxin as a novel, tissue-remodeling-related factor in regressing corpora lutea of cycling rats.

FEBS J 2013 Dec 1;280(24):6600-12. Epub 2013 Nov 1.

Laboratory of Veterinary Physiology, School of Veterinary Medicine, Kitasato University, Towada, Japan.

Autotaxin (ATX) generates lysophosphatidic acid (LPA) from glycerophospholipid via lysophospholipase D (lysoPLD) activity in cooperation with phospholipase A. We studied its expression and possible functional roles in the ovary of nonfertile cycling rats. Immunohistochemistry revealed that ATX was located predominantly in luteal steroidogenic cells of corpora lutea (CL), but not in any follicles. ATX expression was modest in the newest generation of CL and augmented in older generations undergoing structural regression. ATX expression in the whole ovary and lysoPLD activity in circulating blood did not alter during the estrous cycle. Among the LPA receptors examined (LPA1-4 ), LPA4 was densely present on migratory cells, probably phagocytes, at degenerative foci within regressing CL. Bolus administration of anti-ATX IgG or LPA into ovarian bursa in vivo had little effect on the apoptotic cell death of luteal cells, as evaluated by cleaved caspase 3 expression, but led to altered numbers of neutrophils and macrophages in regressing CL, as evaluated by immunological detection of each cell marker. These treatments, together with bromodeoxy uridine, revealed a stimulatory effect of the ATX/LPA pathway on fibroblast proliferation in regressing CL. The results indicate that ATX is increasingly expressed by structurally regressing CL and has definite local action on phagocyte recruitment and fibroblast proliferation which are responsible for tissue remodeling.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/febs.12565DOI Listing
December 2013

Fashion victim: severe tongue burn from a tanning machine.

Burns 2009 Feb 30;35(1):146-7. Epub 2008 Oct 30.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.burns.2008.01.008DOI Listing
February 2009

Oncocytic carcinoma of the parotid gland.

J Otolaryngol 2006 Jun;35(3):189-92

Department of Otorhinolaryngology, National Tokyo Medical Center, 2-5-1 Higashigaoka, Meguro-ku, Tokyo 152-8902, Japan.

View Article and Find Full Text PDF

Download full-text PDF

Source
June 2006

Critical spontaneous rupture of a common carotid artery pseudo-aneurysm.

Lancet 2005 Dec;366(9502):2034

Department of Otolaryngology, National Tokyo Medical Centre, Higashigaoka 2-5-1, Meguro-ku, Tokyo 152-18902, Japan.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/S0140-6736(05)67815-4DOI Listing
December 2005
-->