Publications by authors named "Kan Ding"

139 Publications

Non-lesional mesial temporal lobe epilepsy requires bilateral invasive evaluation.

Epilepsy Behav Rep 2021 27;15:100441. Epub 2021 Mar 27.

Department of Neurology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390-8508, USA.

Purpose: Mesial temporal lobe epilepsy (MTLE) usually responds well to surgical treatment, although in non-lesional cases up to 50% of patients experience seizure relapse. The possibility of bilateral independent seizure onset should be considered as a reason for epilepsy surgery failure.

Methods: In a cohort of 177 patients who underwent invasive presurgical evaluation with stereo-tactically placed electrodes in two level four epilepsy centers, 29 had non-lesional MTLE. Invasive evaluation results are described.

Results: Among 29 patients with non-lesional MRI and mesial temporal lobe seizure onset recorded during stereo-EEG (SEEG) evaluation, four patients with unilateral preimplantation hypothesis had independent bilateral mesial temporal seizures on SEEG despite of unilateral non-invasive evaluation data. Three of these patients were treated with bitemporal responsive neurostimulator system (RNS). Independent bilateral mesial temporal seizures have been confirmed on RNS ECoG (electrocorticography). The fourth patient underwent right anterior temporal lobectomy.

Conclusion: We propose that patients with non-lesional mesial temporal lobe epilepsy would benefit from bilateral invasive evaluation of mesial temporal structures to predict those patients who would be at most risk for surgical failure. Neurostimulaiton could be an initial treatment option for patients with independent bitemporal seizure onset.
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http://dx.doi.org/10.1016/j.ebr.2021.100441DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058515PMC
March 2021

Physical activity status and quality of life in patients with epilepsy - Survey from level four epilepsy monitoring units.

Epilepsy Res 2021 Apr 9;173:106639. Epub 2021 Apr 9.

Department of Neurology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, TX, 75390, USA. Electronic address:

Purpose: People with epilepsy (PWE) tend to have sedentary lifestyles which may predispose them to a lower perceived quality of life (QOL). Moreover, the relationship between physical activity (PA) and QOL in populations of PWE with high disease burden has been under-studied. The goal of this study was to evaluate PA level and its impact on health-related QOL in PWE who were admitted to Level-4 epilepsy monitoring units (EMU).

Methods: In this prospective observational study, 200 patients from two EMUs in Dallas, Texas completed the following standard surveys: Rapid Assessment of Physical Activity (RAPA), the Quality of Life in Epilepsy (QOLIE-31), Patient Health Questionnaire-9 (PHQ-9), and Generalized Anxiety Disorder 7-item (GAD-7) questionnaire. Information on self-reported epilepsy history, severity of disease, and socioeconomic status were also collected. The diagnosis of epilepsy was confirmed by video-EEG monitoring.

Results: Among the 200 who completed the survey, 113 had a diagnosis of epilepsy and 109 of them completed the RAPA. Ninety-two (84 %) of these PWE reported a sedentary level of physical activity (RAPA < 6) and 16 % reported an active level (RAPA ≥ 6). Self-reported QOL was slightly higher in PWE with an active level of PA compared to PWE with a sedentary level of PA (63.8 ± 15.0 vs 53.7 ± 17.9, p = 0.07), even though there was no difference in the severity of self-reported mood symptoms. After controlling for employment and seizure frequency, physical activity level measured by RAPA score was also positively related to QOL (r = 0.39, p = 0.01) and negatively correlated with anxiety symptoms (r = -0.28, p = 0.02) and depression symptoms (r = -0.25, p = 0.04).

Conclusion: The majority of PWE in this survey reported sedentary lifestyles despite most of them being young to middle-aged adults. Higher PA level was associated with fewer self-reported mood symptoms and higher QOL. In conjunction with the literature, these results suggest that PWE with a wide range of disease burden should be encouraged to participate in regular exercise to potentially improve QOL.
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http://dx.doi.org/10.1016/j.eplepsyres.2021.106639DOI Listing
April 2021

Crataegus pinnatifida polysaccharide alleviates colitis via modulation of gut microbiota and SCFAs metabolism.

Int J Biol Macromol 2021 Mar 24;181:357-368. Epub 2021 Mar 24.

Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Science, No.19A Yuquan Road, Beijing 100049, PR China. Electronic address:

Inflammatory bowel disease (IBD) afflicted individual and most medications have side-effects. Crataegus pinnatifida (Hawthorn), which is a safe medicine and food homolog plant, has been reported to prevent colitis in murine. Yet the bioactivity component and the underlying molecular mechanism remain unclear. Here, we established a direct link between colitis induced by dextran sulphate sodium (DSS) in mice and polysaccharide HAW1-2 isolated from hawthorn. Our results showed HAW1-2 restored the pathological lesions in colon and inhibited the expression of inflammatory cytokines including IL-1β, IL-6 and TNF-α. Meanwhile, IKKα/β, IκBα, NF-κB and the phosphorylation levels were inhibited significantly. These findings suggested HAW1-2 could alleviate the inflammation of colon. Further, we found the composition of gut microbiota was modified and Bacteroides including Alistipes and Odoribacter were significantly enriched. Besides, we showed Alistipes and Odoribacter were positively co-related with acetic acid and propionic acid while were negatively co-related with inflammatory cytokines. Finally, we demonstrated the anti-inflammation activity of HAW1-2 might be induced by acetic acid. Together, the present data revealed HAW1-2 could directly modify the gut microbiota, especially for Bacteroides, and generate SCFAs to inhibit colitis. It also implies microbiota-directed intervention in IBD patients should be particularly given more attention.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.03.137DOI Listing
March 2021

Biomarkers of memory variability in traumatic brain injury.

Brain Commun 2021 15;3(1):fcaa202. Epub 2020 Dec 15.

Department of Psychology, University of Pennsylvania, Philadelphia, PA 19104, USA.

Traumatic brain injury is a leading cause of cognitive disability and is often associated with significant impairment in episodic memory. In traumatic brain injury survivors, as in healthy controls, there is marked variability between individuals in memory ability. Using recordings from indwelling electrodes, we characterized and compared the oscillatory biomarkers of mnemonic variability in two cohorts of epilepsy patients: a group with a history of moderate-to-severe traumatic brain injury ( = 37) and a group of controls without traumatic brain injury ( = 111) closely matched for demographics and electrode coverage. Analysis of these recordings demonstrated that increased high-frequency power and decreased theta power across a broad set of brain regions mark periods of successful memory formation in both groups. As features in a logistic-regression classifier, spectral power biomarkers effectively predicted recall probability, with little difference between traumatic brain injury patients and controls. The two groups also displayed similar patterns of theta-frequency connectivity during successful encoding periods. These biomarkers of successful memory, highly conserved between traumatic brain injury patients and controls, could serve as the basis for novel therapies that target disordered memory across diverse forms of neurological disease.
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http://dx.doi.org/10.1093/braincomms/fcaa202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7850041PMC
December 2020

A proof-of-concept trial of a community-based aerobic exercise program for individuals with traumatic brain injury.

Brain Inj 2021 Jan 1;35(2):233-240. Epub 2021 Jan 1.

Department of Neurology, University of Texas Southwestern Medical Center, Dallas, TX, USA.

: To assess the feasibility of conducting an aerobic exercise training study in a community setting for individuals with traumatic brain injury (TBI): This is a prospective, randomized, and controlled study. Nine participants (three moderate-to-severe and six mild TBI) were randomized to a community-based 3-month individualized aerobic exercise training program (AET). Seven participants (four moderate-to-severe, three mild TBI) were randomized to a stretching and toning program (SAT). Cardiorespiratory fitness (CRF) level was assessed with peak oxygen uptake (VO) testing.: After 3 months of training, the AET trended toward improved VO when compared with the SAT group (8% vs - 4%, = .059) with a large effect size of 1.27. Only 50% of participants in the AET group completed more than 70% of the assigned exercise sessions. No adverse events were reported. Both the AET and SAT groups reported small improvements in self-reported mood symptoms, including depression, anxiety, and anger.: It is feasible to conduct an exercise training study and improve CRF for persons with TBI in community settings with structured exercise protocols. However, exploring methods to enhance adherence is crucial for future exercise clinical trials to improve brain health in this population.
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http://dx.doi.org/10.1080/02699052.2020.1865569DOI Listing
January 2021

Fast Organic Vapor Phase Deposition of Thin Films in Light-Emitting Diodes.

ACS Nano 2020 Oct 5;14(10):14157-14163. Epub 2020 Oct 5.

Department of Materials Science and Engineering, University of Michigan, Ann Arbor, Michigan 48109, United States.

Fast deposition of thin films is essential for achieving low-cost, high-throughput phosphorescent organic light-emitting diode (PHOLED) production. In this work, we demonstrate rapid and uniform growth of semiconductor thin films by organic vapor phase deposition (OVPD). A green PHOLED comprising an emission layer (EML) grown at 50 Å/s with bis[2-(2-pyridinyl-)phenyl-](acetylacetonato)iridium(III) (Ir(ppy)(acac)) doped into 4,4'-bis(-carbazolyl)-1,1'-biphenyl (CBP) exhibits a maximum external quantum efficiency of 20 ± 1%. The morphology, charge transport properties, and radiative efficiency under optical and electrical excitation of the PHOLED EML are investigated as functions of the deposition rate both experimental and theoretical approaches. The EML shows no evidence for gas phase nucleation of the organic molecules at deposition rates as high as 50 Å/s. However, the roll-off in quantum efficiency at high current progressively increases with deposition rate due to enhanced triplet-polaron annihilation. The roll-off results from accumulation of stress within the PHOLED EML that generates a high density of defect states. The defects, in turn, act as recombination sites for triplets and hole polarons, leading to enhanced triplet-polaron annihilation at high current. We introduce a void nucleation model to describe the film morphology evolution that is observed using electron microscopy.
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http://dx.doi.org/10.1021/acsnano.0c07017DOI Listing
October 2020

Characterization of anti-BCG benz[α]anthraquinones and new siderophores from a Xinjiang desert-isolated rare actinomycete Nocardia sp. XJ31.

Appl Microbiol Biotechnol 2020 Oct 24;104(19):8267-8278. Epub 2020 Aug 24.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.

The current global demand for novel anti-TB drugs has drawn urgent attention on the discovery of natural product compounds with anti-TB activity. Lots of efforts have emphasized on environmental samples from unexplored or underexplored natural habits and identified numerous rare actinomycete taxa producing structurally diverse bioactive natural products. Herein, we report a survey of the rare actinobacteria diversity in Xinjiang region together with the discovery of anti-TB active natural products from these strains. We have collected 17 soil samples at different sites with different environmental conditions, from which 39 rare actinobacteria were identified by using a selective isolation strategy with 5 media variations. Among those isolated strains, XJ31 was identified as a new Nocardia sp. based on 16S rRNA gene analysis. Through one strain-many compounds (OSMAC) strategy combined with anti-Bacillus Calmette-Guérin bioassay-guided isolation, two groups of compounds were identified. They were twelve siderophores (nocardimicins, 1-12) and two anthraquinones (brasiliquinones, 13 and 14) and ten of them were identified as new compounds. The structures of the purified compounds were elucidated using HR-ESI-MS, 1D NMR, and 2D NMR techniques. The anti-TB bioassays revealed that the two benz[α]anthraquinones have potent activity against BCG (MICs = 25 μM), which can be used as a promising start point for further anti-TB drug development. KEY POINTS: • Ten new natural products were identified from Nocardia sp. XJ31. • Brasiliquinones 13 and 14 showed moderate anti-BCG activity.
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http://dx.doi.org/10.1007/s00253-020-10842-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7443361PMC
October 2020

Nostoc sphaeroids Kütz polysaccharide and powder enrich a core bacterial community on C57BL/6j mice.

Int J Biol Macromol 2020 Nov 8;162:1734-1742. Epub 2020 Aug 8.

Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Science, No.19A Yuquan Road, Beijing 100049, PR China. Electronic address:

Gut microbiota is the collection of microbes that lives in the host. Glycan is the major factor to shape the composition of microbial community. Nostoc sphaeroids Kütz (NSK) has been used as food and medicine for thousands of years in Asian countries while the bioactivity on gut microbiota is unclear till now. Here, we used NSK polysaccharide and NSK powder to investigate the bioactivity on the gut microbiota of C57BL/6j mice, respectively. By 16S ribosomal RNA gene sequencing, we found the composition of gut microbiota had been changed and differed from each other. However, the abundance of Bacteroides, Parabacteroides, Escherichia-Shigella and Parasutterella on genus level were significantly increased by NSK polysaccharide and NSK powder. In addition, Akkermansia and Rikenellaceae were enriched by NSK powder. Moreover, we found the IL-1β and IL-6 decreased significantly while TNF-α and IL-10 increased significantly especially in NSK powder group. Intriguingly, the increased microbes were significantly positively co-related with TNF-α and IL-10 while negatively co-related with IL-1β and IL-6 by co-relation and network analysis. The above results suggested that Nostoc sphaeroids Kütz may selectively enrich a "core bacterial community" and add new evidence to discover how Nostoc sphaeroids Kütz has biological function.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.08.026DOI Listing
November 2020

The impact of structural modification of sulfated polysaccharides on bone morphogenic protein 2 and inhibition of endothelial cell angiogenesis.

Carbohydr Res 2020 Oct 19;496:108093. Epub 2020 Jul 19.

Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai, 201203, PR China; University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing, 100049, PR China. Electronic address:

Sulfated polysaccharides play important roles in angiogenesis. However, the impact of structural alteration of sulfated polysaccharide on the bioactivity is still vague. In this study, binding between different sulfated polysaccharides and bone morphogenic protein 2 (BMP2) was measured to understand the sense of this motif transformation. The results showed that binding between sulfated α-1,4-glucan and BMP2 was the most intensive. The branch of α-1,4-glucan was important for the binding. The affinity of sulfated polysaccharides to BMP2 increased as the molecular weight (MW) and degree of substitution (DS) increased. DS that exceeded 1.05 impaired binding and played more important role in polysaccharide BMP2 interaction than MW. The reservation of partial 6-OH would benefit its binding ability to BMP2. Further, we showed that sulfated polysaccharides with strong binding to BMP2 blocked phosphorylation of Smad 1/5/8 and expression of Id1 to a greater extent than those not strongly bind to BMP2. The binding strength of polysaccharides to BMP2 increased, so did the potency of the anti-angiogenesis effects.
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http://dx.doi.org/10.1016/j.carres.2020.108093DOI Listing
October 2020

Corn silk crude polysaccharide exerts anti-pancreatic cancer activity by blocking the EGFR/PI3K/AKT/CREB signaling pathway.

Food Funct 2020 Aug 22;11(8):6961-6970. Epub 2020 Jul 22.

Glycochemistry & Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.

Pancreatic cancer is a highly lethal and malignant solid tumor. Treatments for pancreatic cancer are seriously limited because it is highly drug-resistant and immunosuppressive. Hence, it is urgent to explore novel approaches for anti-pancreatic cancer therapy. In this study, we show that S1, a crude polysaccharide from corn silk, may significantly inhibit pancreatic cancer cell proliferation in vitro and in vivo. Further studies reveal that S1 can induce pancreatic cancer cell apoptosis, arrest the cell cycle in S phase and impede pancreatic cancer cell migration and invasion. Moreover, S1 may block the EGFR/PI3K/AKT/CREB signaling pathway to exert its anti-pancreatic cancer activity. However, S1 has almost no toxicity either in vitro or in vivo. These results provide evidence that S1 is a new functional food component that can be developed to fight pancreatic cancer.
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http://dx.doi.org/10.1039/d0fo00403kDOI Listing
August 2020

A pectin-like polysaccharide from Polygala tenuifolia inhibits pancreatic cancer cell growth in vitro and in vivo by inducing apoptosis and suppressing autophagy.

Int J Biol Macromol 2020 Nov 9;162:107-115. Epub 2020 Jun 9.

Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, China; University of Chinese Academy of Science, No. 19A Yuquan Road, Beijing 100049, China. Electronic address:

Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal malignant carcinomas, which is characterized by apoptosis- and autophagy-dependent tumorigenic growth. Autophagy constitutes a stress adaptation that suppresses apoptosis. To explore new leading compound against PDAC, a pectin-like polysaccharide named RP02-1, was purified from roots of Polygala tenuifolia. Bioactivity test showed that RP02-1 might inhibit pancreatic cancer cells growth in vitro and in vivo. RP02-1 could inhibit pancreatic cancer cell (AsPC-1 and BxPC-3) proliferation, migration and colony formation. Mechanism study suggested that RP02-1 induced pancreatic cancer cells apoptosis, which was detected by Bcl-2 down-regulation, Bax up-regulation and conversion from Caspase 3 to Cleaved Caspase 3. Interestingly, autophagy was suppressed by RP02-1 treatment concentration-dependently through affenuatingBeclin-1, ATG5 and LC3B expression in BxPC-3 cells. In addition, RP02-1 could inhibit autophagy induced by Pennogenin 3-O-beta-chacotrioside. However, RP02-1 had almost no toxicity both in vitro and in vivo. The above results suggested that RP02-1 might be a potential leading compound for new drug candidate development for human PDAC treatment via inducing apoptosis and against autophagy.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.06.054DOI Listing
November 2020

Impaired cerebral blood flow regulation in chronic traumatic brain injury.

Brain Res 2020 09 4;1743:146924. Epub 2020 Jun 4.

Department of Neurology and Neurotherapeutics, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, TX 75390, USA; Institute for Exercise and Environmental Medicine, Texas Health Presbyterian Hospital Dallas, 8200 Walnut Hill Ln, Dallas, TX 75231, USA.

Baroreflex sensitivity (BRS) and cerebral autoregulation (CA) play an important role in maintaining constant cerebral blood flow (CBF) during systemic changes in blood pressure (BP). Impaired BRS and CA have been reported in acute traumatic brain injury (TBI) which may also contribute to secondary injury and poorer recovery after acute TBI; however, their status during chronic stages remains elusive. Thus, the goal of this study is to determine whether cardiac BRS and dynamic CA (dCA) were impaired during the chronic stage in patients with single TBI and persistent neurological symptoms. Twenty-two subjects with blunt head TBI ≥ 6 months prior to the study (13 mild and 9 moderate to severe TBI) and persistent symptoms on Rivermead Post-Concussion Symptoms Questionnaire at enrollment were compared to 22 age/sex/fitness level-matched healthy control subjects. Beat-to-beat changes in heart rate, BP, and CBF velocity were measured at rest and during a repeated sit-stand maneuver. Hemodynamic variability, dCA, and cardiac BRS were calculated using spectral and transfer function analyses. We found dCA phase in low frequency (LF) range of 0.07-0.20 Hz was lower in subjects with TBI than in control subjects (0.51 ± 0.19 vs. 0.63 ± 0.26, p = 0.043) during the resting condition. Among subjects with TBI, the lower dCA phase in LF was correlated with poorer performance on measures of cognitive function (all p < 0.05). These findings suggested that subjects with chronic TBI showed impaired dCA which may contribute to persistent cognitive impairment. Cerebrovascular measures may provide a physiological measure to evaluate interventions for chronic TBI and accompanying functional deficits.
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http://dx.doi.org/10.1016/j.brainres.2020.146924DOI Listing
September 2020

Ultralong-Range Energy Transport in a Disordered Organic Semiconductor at Room Temperature Via Coherent Exciton-Polariton Propagation.

Adv Mater 2020 Jul 2;32(28):e2002127. Epub 2020 Jun 2.

Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI, 48109, USA.

Amorphous molecular solids are inherently disordered, exhibiting strong exciton localization. Optical microcavities containing such disordered excitonic materials have been theoretically shown to support both propagating and localized exciton-polariton modes. Here, the ultrastrong coupling of a Bloch surface wave photon and molecular excitons in a disordered organic thin film at room temperature is demonstrated, where the major fraction of the polaritons are propagating states. The delocalized exciton-polariton has a group velocity as high as 3 × 10 m s and a lifetime of 500 fs, leading to propagation distances of over 100 µm from the excitation source. The polariton intensity shows a halo-like pattern that is due to self-interference of the polariton mode, from which a coherence length of 20 µm is derived and is correlated with phase breaking by polariton scattering. The demonstration of ultralong-range exciton-polariton transport at room temperature promises new photonic and optoelectronic applications such as efficient energy transfer in disordered condensed matter systems.
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http://dx.doi.org/10.1002/adma.202002127DOI Listing
July 2020

A novel pectin from Polygala tenuifolia blocks Aβ aggregation and production by enhancing insulin-degradation enzyme and neprilysin.

Int J Biol Macromol 2020 Oct 28;161:35-43. Epub 2020 May 28.

Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, PR China. Electronic address:

More and more evidences show that pectin polysaccharide may have impact on Aβ one important molecule implicated in Alzhemer's disease pathology. We speculate special structural motif of pectin might have better bioactivity on Aβ. To address this hypothesis, we reported structure and impact of a novel pectin RP02-1 with the molecular weight of 116.0 kDa from roots of Polygala tenuifolia on Aβ aggregation and production and the underlying mechanism. Its structure is characterized as a backbone of alternate 1, 2, 4-linked α-Rhap and 1, 4-linked α-GalpA, with branches of terminal (T) -, 1, 3-,1, 4-, 1, 6- and 1, 3, 6-linked β-Galp, T-, 1, 5- and 1, 3, 5-linked α-Araf substituted at C-4 of 1, 2, 4-linked α-Rhap. Bioactivity study shows that this pectin may significantly block the aggregation of Aβ. We further show that RP02-1 suppresses Aβ production with no apparent cytotoxicity in both CHO/APPBACE1 and HEK293-APPsw cells. Mechanism study demonstrates that RP02-1 may enhance the expression of insulin-degradation enzyme (IDE) and neprilysin (NEP), which are the main enzymes involved in Aβ degradation. These results suggest that RP02-1 may be a candidate leading compound for anti-Alzheimer's disease new drug development by attenuating Aβ production and inhibiting Aβ aggregation.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.05.212DOI Listing
October 2020

Cordycepin inhibits pancreatic cancer cell growth in vitro and in vivo via targeting FGFR2 and blocking ERK signaling.

Chin J Nat Med 2020 May;18(5):345-355

Glycochemistry & Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Cordycepin (3'-deoxyadenosine) from Cordyceps militaris has been reported to have anti-tumor effects. However, the molecular target and mechanism underlying cordycepin impeding pancreatic cancer cell growth in vitro and in vivo remain vague. In this study, we reported functional target molecule of cordycepin which inhibited pancreatic cancer cells growth in vitro and in vivo. Cordycepin was confirmed to induce apoptosis by activating caspase-3, caspase-9 and cytochrome c. Further studies suggested that MAPK pathway was blocked by cordycepin via inhibiting the expression of Ras and the phosphorylation of Erk. Moreover, cordycepin caused S-phase arrest and DNA damage associated with activating Chk2 (checkpoint kinase 2) pathway and downregulating cyclin A2 and CDK2 phosphorylation. Very interestingly, we showed that cordycepin could bind to FGFR2 (K = 7.77 × 10) very potently to inhibit pancreatic cancer cells growth by blocking Ras/ErK pathway. These results suggest that cordycepin could potentially be a leading compound which targeted FGFR2 to inhibit pancreatic cells growth by inducing cell apoptosis and causing cell cycle arrest via blocking FGFR/Ras/ERK signaling for anti-pancreatic cancer new drug development.
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http://dx.doi.org/10.1016/S1875-5364(20)30041-8DOI Listing
May 2020

The homogenous polysaccharide SY01-23 purified from leaf of Morus alba L. has bioactivity on human gut Bacteroides ovatus and Bacteroides cellulosilyticus.

Int J Biol Macromol 2020 May 7;158:698-707. Epub 2020 May 7.

Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Science, No.19A Yuquan Road, Beijing 100049, PR China. Electronic address:

Function of mulberry leaf (Morus alba L.) polysaccharide has been reported on antitumor, immunostimulatory and anti-inflammatory effects. However, the bioactivity on human gut microbiota is unclear so far. Here, three homogenous polysaccharides named SY01-21, SY01-22, SY01-23 were isolated from mulberry leaf with molecular weight 57 kDa, 25 kDa and 7.2 kDa, respectively. The monosaccharide composition of SY01-21 contained rhamnose, galactose and arabinose in a molar ratio of 7.60:43.52:48.88. SY01-22 contained rhamnose, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 14.61:9.06:1.35:34.65:2.99:37.34. SY01-23 contained rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose in a molar ratio of 23.00:4.12:24.60:5.74:17.28:1.12:24.13. Bioactivity test showed SY01-21 promoted the growth of Bacteroides cellulosilyticus (BC) while SY01-22 benefited the growth of Bacteroides ovatus (BO). Interestingly, SY01-23 boosted the growth of both BO and BC. However, Bacteroides thetaiotamicron (BT) only grew on 5 mg/mL SY01-21. Intriguingly, the growth of co-culture of BT with BO or BC was better than monoculture. This suggested that cross-feeding might exist between them. Besides, we found BO and BC generated acetate and propionate by utilizing SY01-23. The above results suggested that SY01-23 might modify human gut microbiota by driving colonization of Bacteroides in the gut to improve wellness.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.05.009DOI Listing
May 2020

Central autonomic network functional connectivity: correlation with baroreflex function and cardiovascular variability in older adults.

Brain Struct Funct 2020 Jun 30;225(5):1575-1585. Epub 2020 Apr 30.

Departments of Radiology and Psychology and Cognitive Imaging Research Center, Michigan State University, Radiology Building, 846 Service Road, East Lansing, MI, 48824, USA.

Baroreflex regulates short-term cardiovascular variability via the autonomic neural system. The contributions of the central autonomic system to the baroreflex regulations of arterial blood pressure (BP) and heart rate have been reported in young healthy adults, but not in older adults. Therefore, we investigated the association between the high-level central autonomic network (CAN) connectivity and baroreflex sensitivity (BRS) under a resting condition in a healthy older population. Twenty-two older adults (68 ± 8 years old) underwent BRS assessment using the modified Oxford and transfer function methods. Resting-state brain functional MRI was performed to assess the CAN functional connectivity at rest. We found that the functional connectivity (FC) between the left amygdala and left medial frontal gyrus (MeFG), bilateral postcentral gyri and bilateral paracentral lobules (PCL) is associated with BRS and R-R interval (RRI) variability in the low-frequency (LF) range. Compared to the left amygdala, the FC map of the right amygdala only showed significant associations with BRS in the anterior cingulate cortex (ACC) and with RRI variability in the left occipital region. In addition, post hoc analysis of the functionally defined left insula sub-region confirmed the association between CAN and BRS. Overall, our study demonstrates that CAN and its related brain regions may be involved, likely in a left-lateral manner, in peripheral cardiac autonomic regulation at rest. The results highlight the potential importance of brain neural network function in maintaining cardiovascular homeostasis in older adults.
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http://dx.doi.org/10.1007/s00429-020-02075-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7292781PMC
June 2020

I-Iofluopane Single-Photon Emission Computed Tomography as an Imaging Biomarker of Pre-Synaptic Dopaminergic System after Moderate-to-Severe Traumatic Brain Injury.

J Neurotrauma 2020 10 3;37(19):2113-2119. Epub 2020 Jun 3.

Department of Neurology and Neurotherapeutics, University of Texas Southwestern Medical Center, Dallas, Texas, USA.

Dopaminergic (DA) system function is frequently disrupted after traumatic brain injury (TBI). However, published interventions that target the DA system with the hope of enhancing functional outcomes are inconclusive, partially because of the lack of DA signaling biomarkers that can be used to select patients likely to benefit from DA-directed therapies or to monitor treatment efficacy. The aim of this study was to evaluate the feasibility of using I-iofluopane single-photon emission computerized tomography (SPECT) to assess pre-synaptic DA system dysfunction after severe TBI. Eighteen patients with severe TBI were enrolled in this study. I-iofluopane SPECT imaging was performed at baseline and again 2.5 h after a single dose of methylphenidate (MP) administered enterally. DA transporter (DAT) specific binding ratio (SBR) before and after MP was measured. Functional outcomes included the Disability Rating Scale, JFK Coma Recovery Scale-Revised, Functional Independence Measure, and Functional Assessment Measure. Thirteen of 18 patients completed the study. Average time from injury to SPECT scan was 48 days (standard deviation [SD], 24 days; median, 31). Baseline ioflupane striatal SBR was 1.51 ± 0.46 (median, 1.67). A 43.1% (SD, 16; median, 46.5) displacement of ioflupane from pre-synaptic DAT was observed after MP administration. Baseline SBR positively correlated with functional status at baseline and 4 weeks after completion of the study. Serum MP levels correlated with relative change in SBR ( = 0.60;  = 0.04). Our findings suggest that I-iofluopane SPECT is a promising tool to determine the severity of pre-synaptic DA terminal disruption and for monitoring pharmacokinetics and pharmacodynamics of therapeutic interventions targeting the DA system.
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http://dx.doi.org/10.1089/neu.2019.6892DOI Listing
October 2020

Structural elucidation of a pectin from roots of Polygala tenuifolia and its neuritogenesis inducing activity in PC12 cells.

Carbohydr Polym 2020 May 20;236:116048. Epub 2020 Feb 20.

Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, PR China. Electronic address:

Pectin is a family of heteropolysaccharides and highly valued for its various bioactivities. Here, a pectin, RP01-1, was purified from roots of Polygala tenuifolia with the molecular weight of 79.1 kDa. Its structure was characterized as alternate 1, 2, 4-linked α-Rhap and 1, 4-linked α-GalpA constituted the backbone, with branches of terminal (T) -, 1, 4- and 1, 3, 6-linked β-Galp, T-, 1, 5- and 1, 3, 5-linked α-Araf substituted at C-4 of 1, 2, 4-linked α-Rhap. The fluorescence spectroscopic analysis indicated that RP01-1 induced neurite outgrowth in PC12 cells and primary cortex neurons in dose dependent manner with no significant cytotoxicity. To disclose the mechanism of this phenomenon, we found that RP01-1 might induce neuritogenesis in PC12 cells via increasing BDNF expression, and promoting the phosphorylation of AKT, ERK, CREB. The results suggest that AKT, ERK, CREB related signaling pathways might implicate in neuritogenesis-inducing activity of RP01-1.
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http://dx.doi.org/10.1016/j.carbpol.2020.116048DOI Listing
May 2020

Isolation and structure characterization of a polysaccharide from Crataegus pinnatifida and its bioactivity on gut microbiota.

Int J Biol Macromol 2020 Jul 10;154:82-91. Epub 2020 Mar 10.

Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Science, No.19A Yuquan Road, Beijing 100049, PR China. Electronic address:

A glucose rich heteroglycan named HAW1-2 was isolated and purified from fruit of Crataegus pinnatifida by hot-water extraction, ethanol precipitation, DEAE-cellulose anion exchange and gel permeation chromatography. HAW1-2 was proved as a homogenous polysaccharide with a molecular weight (Mw) of 8.94 kDa. Monosaccharide composition results indicated that HAW1-2 was composed of arabinose, galactose and glucose. Methylation analysis and NMR spectrum showed that HAW1-2 contained →4)-β-d-Glcp-(1→, →4)-β-d-Galp-(1→, α-L-Araf-(1→, →5)-α-L-Araf-(1→, β-d-Glcp-(1→ and →4,6)-β-d-Glcp-(1→. Bioactivity test showed that HAW1-2 could significantly promote the growth of Bacteroides thetaiotamicron (BT), Bacteroides ovatus (BO) and Bifidobacterium longum (BL), which showed competition with the Bacteroides spp. under co-culture. Interestingly, Bacteroides spp. generated more acetic acids and propionic acids while BL only generated acetic acids. These results imply that polysaccharide HAW1-2 may be useful for human by modulating intestinal bacteria and producing short chain fatty acids.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.03.058DOI Listing
July 2020

[Effect of the contralateral needling therapy on post-stroke shoulder-hand syndrome].

Zhen Ci Yan Jiu 2020 Feb;45(2):152-6

Institute of Chinese Medicine History and Literature, China Academy of Chinese Medical Sciences, Beijing 100700.

Objective: To observe the effect contralateral needling of in improving pain, edema and limb dysfunction in stroke patients with shoulder-hand syndrome.

Methods: A total of 62 patients with post-stroke shoulder-hand syndrome were divided into a control group and an observation group, 31 cases in each one. The routine treatment with internal medicine and rehabilitation manipulation was adopted in the two groups. Additionally, the routine acupuncture treatment was used in the control group and the contralateral needling techniques was applied in the observation group. The needles were retained for 30 min. The treatment was given once a day for 5 days a week and consecutively for 4 weeks. Before and after treatment, the score of the modified Fugl-Meyer assessment scale (FMA scale), the score of the visual analogue scale (VAS), the score of the hand edema rating and the score of the modified Barthel index (ADL score) were evaluated.

Results: The total effective rate was 90.32% (28/31) in the observation group and was 67.74% (21/31) in the control group. The effective rate in the observation group was higher than that in the control group (<0.05). VAS score, the score of the hand edema rating, FMA score and ADL score were obviously improved as compared with those before treatment in each group and the scores in the observation group were better than those of the control group (<0.05).

Conclusion: The contralateral needling therapy of acupuncture is effective for relieving pain and edema as well as improving the motor function of the affected limb in the patients with post-stroke shoulder-hand syndrome.
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http://dx.doi.org/10.13702/j.1000-0607.1904786DOI Listing
February 2020

Intestinal microbes derived butyrate is related to the immunomodulatory activities of Dendrobium officinale polysaccharide.

Int J Biol Macromol 2020 Apr 1;149:717-723. Epub 2020 Feb 1.

Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China. Electronic address:

Although immunomodulatory activities of Dendrobium officinale polysaccharide has been investigated for many years, yet the potential contribution of its metabolite derived from intestinal microbes on immunoregulation effect has not been reported. In this study, polysaccharide DOW-5B with average molecular weight of 39.4 kDa was isolated from the stem of Dendrobium officinale Kimura et Migo. The carbohydrate content was 91.97% and no protein was detected. The monosaccharide analysis showed this polysaccharide was composed of glucuronic acid and glucose at a molar ratio (M/G) of 1.2:19.4. Animal test indicated DOW-5B increased the diversity of gut microbiota on mice. Beneficial microbes such as Ruminococcus, Eubacterium, Clostridium, Bifidobacterium, Parabacteroides and Akkermansiamuciniphila increased while harmful bacteria in Proteobacteria decreased. Surprisingly, DOW-5B promoted gut microbes to generate more butyrate and mainly produced by Parabacteroides_sp_HGS0025. Further, we found the health of large intestine as well as immunity response of mice was improved. In addition, Parabacteroides_sp_HGS0025 positively correlated with butyrate, IgM, IL-10, and TNF-α products in intestine and mice blood, respectively. The data suggested that Dendrobium officinale polysaccharide has function on immunity may be mediated by butyrate. It adds new evidence to support the basis of how herbal polysaccharides affect immunity.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.01.305DOI Listing
April 2020

A review of isolation methods, structure features and bioactivities of polysaccharides from Dendrobium species.

Chin J Nat Med 2020 Jan;18(1):1-27

Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:

Dendrobium, orchid, is a traditional Chinese herb medicine applied extensively as tonic and precious food for thou-sands of years recorded in ancient Chinese medical book "Shen Nong's Materia Medica". It's well known that bioactivities are usually related to the ingredients' basis. Based on the previous research, Dendrobium species contain amino acid, sesquiterpenoids, alkaloids and polysaccharides. As the bioactive substances, carbohydrate shows extensive activities in antitumor, antiglycation, immune-enhancing, antivirus, antioxidant, antitumor and etc. Therefore, as the main biologically active substance, the exact structures and latent activities of polysaccharides from Dendrobium species are widely focused on. In this review, we focus on the advancements of extraction methods and diversity of structures and bioactivities of polysaccharides obtained from Dendrobium species.
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http://dx.doi.org/10.1016/S1875-5364(20)30001-7DOI Listing
January 2020

An Investigation of Heartrate Sensing Accuracy by Wrist-Worn Fitness Tracking Devices.

Annu Int Conf IEEE Eng Med Biol Soc 2019 Jul;2019:3337-3340

Preliminary results of assessing the accuracy of heartrate measurement by four wrist-worn fitness tracking devices (WFT) during waking and stationary biking in five healthy subjects (2F; age 26.4±3.20 years; BMI 24.7±1.92 kg/m) are reported. The results reveal that the accuracy varies depending on the WFT and exercise mode.
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http://dx.doi.org/10.1109/EMBC.2019.8856375DOI Listing
July 2019

Comparison of psychiatric comorbidities and impact on quality of life in patients with epilepsy or psychogenic nonepileptic spells.

Epilepsy Behav 2020 01 20;102:106649. Epub 2019 Nov 20.

Department of Neurology and Neurotherapeutics, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, TX 75390, USA. Electronic address:

Objectives: Psychiatric comorbidity is common in people with epilepsy (PWE) and psychogenic nonepileptic spells (PNES). These comorbidities can be detrimental to quality of life (QOL) and are often underdiagnosed and undertreated. Some types of epilepsy, such as focal temporal lobe epilepsy (TLE), have been associated with higher rates of psychiatric comorbidity. This study examined the impact of psychiatric comorbidity on QOL in patients admitted to two level 4 epilepsy monitoring units (EMUs).

Methods: In this prospective observational study, 200 patients admitted to two level 4 EMUs completed standardized surveys including the Quality of Life in Epilepsy (QOLIE-31-P), Generalized Anxiety Disorder 7-item (GAD-7), Patient Health Questionnaire (PHQ-9), and Beck Depression Inventory-II (BDI-II). Hierarchal multiple regression was performed to assess impact on QOL.

Results: Of the 200 participants, 113 had a diagnosis of epilepsy, 36 had a diagnosis of PNES, and 51 were excluded for nondiagnostic evaluation or dual diagnosis. Of those with epilepsy, 65 had TLE, 28 had focal extratemporal lobe epilepsy (ETLE), and 20 had nonfocal epilepsy. Patients with PNES had higher self-reported anxiety and depression levels (GAD-7: p = 0.04, PHQ-9: p < 0.01; BDI-II: p < 0.01) but similar QOL to PWE (p = 0.78). Using hierarchal multiple regression, symptoms of anxiety and depression were significant predictors of lower QOL in PWE but not in patients with PNES. There was no difference in QOL in those with ETLE and TLE.

Conclusions: Our findings suggest that self-reported anxiety and depression symptoms are common in patients admitted to level 4 EMUs regardless of diagnosis and play an important role in predicting QOL in PWE. Our findings emphasize the importance of routinely screening all EMU patients for psychiatric comorbidity.
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http://dx.doi.org/10.1016/j.yebeh.2019.106649DOI Listing
January 2020

Polysaccharides from Lycium barbarum ameliorate amyloid pathology and cognitive functions in APP/PS1 transgenic mice.

Int J Biol Macromol 2020 Feb 9;144:1004-1012. Epub 2019 Nov 9.

State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China; Shanghai Key Laboratory of Signaling and Disease Research, Collaborative Innovation Center for Brain Science, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China. Electronic address:

Alzheimer's disease (AD) is the most common degenerative disease of the central nervous system. It is associated with abnormal accumulation of amyloid-β (Aβ) plaques, impaired neurogenesis, and damaged cognitive functions. We have known for a long time that natural compounds and their derivatives have gained increasing attention in AD drug research due to their multiple effects and inherently enormous chemicals. In this study, we will demonstrate that polysaccharides from L. barbarum (LBP1), a traditional natural compound, can reduce Aβ level and improve the cognitive functions in APP/PS1 transgenic mouse. LBP1 can enhance neurogenesis as indicated by BrdU/NeuN double labeling. Furthermore, it can restore synaptic dysfunction at hippocampus CA3-CA1 pathway. Additionally, in vitro cell assay indicates that LBP1 may affect Aβ processing. In conclusion, our study indicates that LBP1 might be a potential therapeutic agent for the treatment of AD against multiple targets that include synaptic plasticity, Aβ pathology and neuropathology.
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http://dx.doi.org/10.1016/j.ijbiomac.2019.09.177DOI Listing
February 2020

Vacuum-Deposited Biternary Organic Photovoltaics.

J Am Chem Soc 2019 Nov 31;141(45):18204-18210. Epub 2019 Oct 31.

Department of Chemistry , National Taiwan University , Taipei 10617 , Taiwan.

Ternary blend organic photovoltaics (OPVs) have been introduced to improve solar spectral absorption and reduce energy losses beyond that of binary blend OPVs, but the difficulties in simultaneously optimizing the morphology of three molecular components result in devices that have generally exhibited performance inferior to that of analogous binary OPVs. Here, we introduce a small molecule-based biternary OPV comprising two individual, vacuum-deposited binary bulk heterojunctions fused at a planar junction without component intermixing. In contrast to previous reports where the open circuit voltage () of a conventional, blended ternary cell lies between those of the individual binaries, the of the biternary OPV corresponds to one of the constituent binaries, depending on the order in which they are stacked relative to the anode. Additionally, dipole-induced energy-level realignment between the two binary segments necessary to achieve maximum efficiency is observed only when using donor-acceptor-acceptor' dipolar donors in the photoactive heterojunctions. The optimized biternary OPV shows improved performance as compared to its two constituent binary OPVs, achieving a power conversion efficiency of 10.6 ± 0.3% under AM 1.5G 1 sun (100 mW/cm) simulated illumination with = 0.94 ± 0.01 V, a short circuit current density of 16.0 ± 0.5 mA cm, and a fill factor of 0.70 ± 0.01.
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http://dx.doi.org/10.1021/jacs.9b09012DOI Listing
November 2019

Structure elucidation and anti-tumor activity in vivo of a polysaccharide from spores of Ganoderma lucidum (Fr.) Karst.

Int J Biol Macromol 2019 Dec 7;141:693-699. Epub 2019 Sep 7.

Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, China.

To explore new anti-tumor constituents in the fungi, a water-soluble polysaccharide, WGLP was obtained from spores of Ganoderma lucidum (Fr.) Karst. The average molecular weight of this polysaccharide was estimated to be 1.5 × 10 Da. Monosaccharide composition analysis indicated that it was composed solely of glucose. From methylation analysis, H and C NMR spectroscopy, and periodate oxidation and Smith degradation, it could be concluded that WGLP contained β-d-glucose residue exclusively, with an average repeating unit of hexasaccharide, having a backbone consisting of (1 → 3)-β-d-glucopyranosyl residues, to which the side chain consisting of terminal and (1 → 6)-β-d-glucopyranosyl residue is attached at position O-6 of the branching residues. Anti-tumor activity assays in vivo showed that WGLP could significantly inhibit the S180 tumor growth in the mice. Furthermore, no drug-related toxic reactions were observed. It was suggested that WGLP might be a potential anti-tumor agent used in the clinic.
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http://dx.doi.org/10.1016/j.ijbiomac.2019.09.046DOI Listing
December 2019

A galactoglucan isolated from of Cistanche deserticola Y. C. Ma. and its bioactivity on intestinal bacteria strains.

Carbohydr Polym 2019 Nov 2;223:115038. Epub 2019 Jul 2.

Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Sciences, No.19A Yuquan Road, Beijing 100049, PR China. Electronic address:

To understand whether polysaccharides may have impact on gut microbiota, a neutral polysaccharide CDA-0.05 with an average molecular weight of 7.96 kDa was obtained from Cistanche deserticola Y. C. Ma. The monosaccharide composition analysis indicated that CDA-0.05 was composed of glucose and galactose in a molar ratio of 96.4: 3.6. The backbone of CDA-0.05 contained 1, 4-linked α-D-Glcp, 1, 4, 6-linked α-D-Glcp and 1, 4-linked β-D-Galp, with branches of T-linked α-D-Glcp attached at C-6 of 1, 4, 6-linked α-D-Glcp residues. Bioactivity test results suggested that CDA-0.05 could promote the growth of three species of Bacteroides (B. thetaiotaomicron, B. ovatus and B. fragilis) significantly. Furthermore, CDA-0.05 could also promote some probiotics growth, such as Lactobacillus casei, Lactobacillus plantarum and Lactobacillus reuteri. These results suggested that CDA-0.05 might help to maintain intestinal homeostasis and could be recommended as part of fibers or drugs candidate to benefit human body by regulating gut bacteria.
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http://dx.doi.org/10.1016/j.carbpol.2019.115038DOI Listing
November 2019

Isolation and structural characterization of a pectin from Lycium ruthenicum Murr and its anti-pancreatic ductal adenocarcinoma cell activity.

Carbohydr Polym 2019 Nov 20;223:115104. Epub 2019 Jul 20.

Glycochemistry and Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai 201203, PR China; University of Chinese Academy of Science, No.19A Yuquan Road, Beijing 100049, PR China. Electronic address:

Crude polysaccharides were obtained from fruits of Lycium ruthenicum Murr using hot water extraction followed by ethanol precipitation. A homogeneous polysaccharide, LRP3-S1 with a relative molecular weight of 114.8 kDa was purified by anion-exchange chromatography on DEAE SepharoseFast Flow and Sephacryl S-300 HR column. Monosaccharide composition analysis revealed that LRP3-S1 was composed of rhamnose, galacturonic acid, galactose, xylose and arabinose in a molar ratio of 14.4: 17.7: 26.6: 16.4: 24.9. LRP3-S1 contained a rhamnogalacturonan I (RG-I) backbone partially substituted at C-4 of rhamnose units by side chains, which included T-linked β-D-Galp, 1,3-linked β-D-Galp, 1,6-linked β-D-Galp, 1,3,6-linked β-D-Galp, 1,5-linked α-L-Araf, 1,3,5-linked α-L-Araf, T-linked α-L-Araf and T-linked β-D-Xylp. Biological activity tests showed that LRP3-S1 could inhibit the growth of pancreatic cancer cells. In addition, LRP3-S1 could attenuate invasion ability of BxPC-3 cells and down-regulate protein expression of p-FAK, p-AKT, p-GSK-3β and p-p38 MAP kinase.
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http://dx.doi.org/10.1016/j.carbpol.2019.115104DOI Listing
November 2019