Publications by authors named "Kamonchai Imnoi"

3 Publications

  • Page 1 of 1

Characterization of a novel picornavirus isolated from moribund aquacultured clownfish.

J Gen Virol 2020 07;101(7):735-745

Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.

Over the last decade, a number of USA aquaculture facilities have experienced periodic mortality events of unknown aetiology in their clownfish (). Clinical signs of affected individuals included lethargy, altered body coloration, reduced body condition, tachypnea, and abnormal positioning in the water column. Samples from outbreaks were processed for routine parasitological, bacteriological, and virological diagnostic testing, but no consistent parasitic or bacterial infections were observed. Histopathological evaluation revealed individual cell necrosis and mononuclear cell inflammation in the branchial cavity, pharynx, oesophagus and/or stomach of four examined clownfish, and large basophilic inclusions within the pharyngeal mucosal epithelium of one fish. Homogenates from pooled external and internal tissues from these outbreaks were inoculated onto striped snakehead (SSN-1) cells for virus isolation and cytopathic effects were observed, resulting in monolayer lysis in the initial inoculation and upon repassage. Transmission electron microscopy of infected SSN-1 cells revealed small round particles (mean diameter=20.0-21.7 nm) within the cytoplasm, consistent with the ultrastructure of a picornavirus. Full-genome sequencing of the purified virus revealed a novel picornavirus most closely related to the bluegill picornavirus and other members of the genus . Additionally, pairwise protein alignments between the clownfish picornavirus (CFPV) and other known members of the genus yielded results in accordance with the current International Committee on Taxonomy of Viruses criteria for members of the same genus. Thus, CFPV represents a proposed new limnipivirus species. Future experimental challenge studies are needed to determine the role of CFPV in disease.
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http://dx.doi.org/10.1099/jgv.0.001421DOI Listing
July 2020

Characterization of ranaviruses isolated from lumpfish L. in the North Atlantic area: proposal for a new ranavirus species (European North Atlantic Ranavirus).

J Gen Virol 2020 02 20;101(2):198-207. Epub 2019 Dec 20.

Technical University of Denmark, National Institute of Aquatic Resources, Aquatic Animal Health Unit, Kgs. Lyngby, Denmark.

The commercial production of lumpfish L. is expanding with the increased demand for their use as cleaner fish, to control sea-lice numbers, at marine Atlantic salmon L. aquaculture sites throughout Northern Europe. A new ranavirus has been isolated from lumpfish at multiple locations in the North Atlantic area. First isolated in 2014 in the Faroe Islands, the virus has subsequently been found in lumpfish from Iceland in 2015 and from Scotland and Ireland in 2016. The Icelandic lumpfish ranavirus has been characterized by immunofluorescent antibody test, optimal growth conditions and transmission electron microscopy. Partial sequences of the major capsid protein gene from 12 isolates showed 99.79-100% nt identity between the lumpfish ranaviruses. Complete genome sequencing from three of the isolates and phylogenetic analysis based on the concatenated 26 iridovirus core genes suggest these lumpfish ranavirus isolates form a distinct clade with ranaviruses from cod L. and turbot L. isolated in Denmark in 1979 and 1999, respectively. These data suggest that these viruses should be grouped together as a new ranavirus species, European North Atlantic Ranavirus, which encompasses ranaviruses isolated from marine fishes in European North Atlantic waters.
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http://dx.doi.org/10.1099/jgv.0.001377DOI Listing
February 2020

Genomic Sequencing of Ranaviruses Isolated from Edible Frogs ().

Genome Announc 2017 Sep 21;5(38). Epub 2017 Sep 21.

Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.

Ranaviruses were isolated from wild edible frogs () during epizootics in Denmark and Italy. Phylogenomic analyses revealed that these isolates are closely related and belong to a clade of ranaviruses that includes the ranavirus (ADRV), common midwife toad ranavirus (CMTV), ranavirus (THRV), and pike-perch iridovirus (PPIV).
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http://dx.doi.org/10.1128/genomeA.01015-17DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5609427PMC
September 2017