Publications by authors named "Kaiyang Li"

35 Publications

Delivery of manganese carbonyl to the tumor microenvironment using Tumor-Derived exosomes for cancer gas therapy and low dose radiotherapy.

Biomaterials 2021 07 18;274:120894. Epub 2021 May 18.

Department of Electronic Science and Technology, School of Physics and Technology, Wuhan University, Wuhan, 430072, China.

The development of novel radiosensitizer with high selectivity and controllability is highly desirable. CO gas could cause damage to mitochondria and thus enhance RT effect. Controlled delivery of CO in tumor is important both to achieve high-efficiency of CO gas therapy and to decrease the risk of CO poisoning. In this study, manganese carbonyl (MnCO) loaded exosome nano-vesicles (MMV) to overcome this conundrum for tumor therapy is developed. After administration, MMV showed its admirable performance in active tumor-targeting, mitochondria damage and radiosensitization therapy. These MMV nanoparticles were able to facilitate robust CO evolution and consequent ROS generation in response to X-ray irradiation both in vitro and in vivo. Significantly, MMV could facilitate a 90% inhibition effect of tumor growth under very low dose (only 2Gy) RT, which is better than high dose (6Gy) radiotherapy. Overall, this study highlights a novel and practical approach to enhancing the efficacy of tumor RT, underscoring the value of future research in the field of CO medicine.
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http://dx.doi.org/10.1016/j.biomaterials.2021.120894DOI Listing
July 2021

A biomimetic nanozyme/camptothecin hybrid system for synergistically enhanced radiotherapy.

J Mater Chem B 2020 06;8(24):5312-5319

Department of Electronic Science and Technology, School of Physics and Technology, Wuhan University, Wuhan 430072, China.

Although radiotherapy (RT) has been an effective therapeutic regimen against most solid tumors, its effect is limited by the hypoxic tumor microenvironment and radio-tolerance of tumor cells to a large extent. Here we have designed a biomimetic nanozyme/camptothecin hybrid system for synergistically enhanced radiotherapy, which consists of an internal camptothecin (CPT)-loaded hollow MnO2 core and an external tumor cell membrane. The tumor cell membrane endows the system with excellent tumor targeting ability. The hollow MnO2 core can deliver the hydrophobic drug CPT and catalyze the production of oxygen from hydrogen peroxide in tumor tissues, which was finally degraded into Mn2+, a T1-weighted contrast agent. The anti-tumor mechanism of this system includes two aspects: (i) the generated oxygen can improve the hypoxic state of the tumor microenvironment and enhance the radiotherapy sensitivity and (ii) CPT can induce cell cycle arrest in the S-phase at a low dose, which further increases the radio-sensitivity of tumor cells and augmented radiation-induced tumor damage. The results of in vivo experiments showed that the biomimetic nanozyme drug delivery system improved the hypoxic microenvironment of the tumor tissue with a high tumor inhibition rate in a murine model. This platform achieved synergistic radiotherapy sensitization and provided a novel idea for the design of a radiotherapy sensitization system.
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http://dx.doi.org/10.1039/d0tb00676aDOI Listing
June 2020

The Genetic Architecture of Early Body Temperature and Its Correlation With Resistance in Three Chicken Breeds.

Front Genet 2019 22;10:1287. Epub 2020 Jan 22.

State Key Laboratory of Animal Nutrition, Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.

New-born chicks are vulnerable to bacterial infections and not good at regulating body temperature. There is a close relationship between thermal regulation and immunity, however, the underlying mechanism is not well understood. (SP) is a major concern in developing countries and causes significant economic losses in poultry industry. Early body temperature (EBT) has previously shown to be correlated with host immunity and resistance to pullorum disease. In this study, we challenged three independent chick populations (Beijing You, Dwarf and Rhode Island Red) with SP at 4 days of age, and rectal temperature was measured before and after the SP attack from 2 to 7 days of age. Host defense to SP was evaluated by survival and spleen SP carrier status. The results showed that chicks with higher EBT before SP infection tend to have higher resistance to later SP attack in two populations (Dwarf and Beijing You). The association between EBT before SP attack and SP resistance was non-significant in Rohde Island Red population ( = 0.06), but the trend was consistent with the other two populations. We also found low to moderate heritability in all three populations for EBT before and after the SP attack ranging from 0.14 to 0.20. Genome-wide association studies identified several genomic regions and biological pathways determining EBT before SP attack, which provides candidate functional genes of this trait. Our results reveal the genetic determination of EBT, and the relationship between EBT and SP resistance, providing an alternative strategy for improving SP resistant activities in chicken.
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http://dx.doi.org/10.3389/fgene.2019.01287DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6987447PMC
January 2020

A genome-wide association study explores the genetic determinism of host resistance to Salmonella pullorum infection in chickens.

Genet Sel Evol 2019 Sep 18;51(1):51. Epub 2019 Sep 18.

State Key Laboratory of Animal Nutrition, Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.

Background: Salmonella infection is a serious concern in poultry farming because of its impact on both economic loss and human health. Chicks aged 20 days or less are extremely vulnerable to Salmonella pullorum (SP), which causes high mortality. Furthermore, an outbreak of SP infection can result in a considerable number of carriers that become potential transmitters, thus, threatening fellow chickens and offspring. In this study, we conducted a genome-wide association study (GWAS) to detect potential genomic loci and candidate genes associated with two disease-related traits: death and carrier state.

Methods: In total, 818 birds were phenotyped for death and carrier state traits through a SP challenge experiment, and genotyped by using a 600 K high-density single nucleotide polymorphism (SNP) array. A GWAS using a single-marker linear mixed model was performed with the GEMMA software. RNA-sequencing on spleen samples was carried out for further identification of candidate genes.

Results: We detected a region that was located between 33.48 and 34.03 Mb on chicken chromosome 4 and was significantly associated with death, with the most significant SNP (rs314483802) accounting for 11.73% of the phenotypic variation. Two candidate genes, FBXW7 and LRBA, were identified as the most promising genes involved in resistance to SP. The expression levels of FBXW7 and LRBA were significantly downregulated after SP infection, which suggests that they may have a role in controlling SP infections. Two other significant loci and related genes (TRAF3 and gga-mir-489) were associated with carrier state, which indicates a different polygenic determinism compared with that of death. In addition, genomic inbreeding coefficients showed no correlation with resistance to SP within each breed in our study.

Conclusions: The results of this GWAS with a carefully organized Salmonella challenge experiment represent an important milestone in understanding the genetics of infectious disease resistance, offer a theoretical basis for breeding SP-resistant chicken lines using marker-assisted selection, and provide new information for salmonellosis research in humans and other animals.
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http://dx.doi.org/10.1186/s12711-019-0492-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6751821PMC
September 2019

Light transport in homogeneous tissue with m-dependent anisotropic scattering I: Case's singular eigenfunctions solution and Chandrasekhar polynomials.

Biomed Opt Express 2018 Sep 2;9(9):4009-4030. Epub 2018 Aug 2.

Wuhan University, School of Physics and Technology, Department of Electronic Science and Technology, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province 430072, China.

This paper is the first of two deriving the analytical solutions for light transport in infinite homogeneous tissue with an azimuth-dependent (m-dependent) anisotropic scattering kernel by two approaches, Case's singular eigenfuncions expansion and Fourier transform, as well as proving the consistence of the two solutions. In this paper, Case's method was applied and extended to the general m-dependent anisotropic scattering case. The explicit Green's function of radiance distributions, which was regarded as the comparative standard for the equivalent solution via Fourier transform and inversion in our second accompanying paper, was expanded into a complete set of the discrete and continuous eigenfunctions. Considering that the two kinds of m-dependent Chandrasekhar orthogonal polynomials that play vital roles in these analytical solutions are very sensitive to the typical optical parameters of biological tissue as well as the degrees or orders, four numerical evaluation methods were benchmarked to find the stable, reliable and feasible numerical evaluation methods in high degrees and high orders.
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http://dx.doi.org/10.1364/BOE.9.004009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157773PMC
September 2018

Light transport in homogeneous tissue with m-dependent anisotropic scattering II: Fourier transform solution and consistent relations.

Biomed Opt Express 2018 Sep 2;9(9):4031-4053. Epub 2018 Aug 2.

Wuhan University, School of Physics and Technology, Department of Electronic Science and Technology, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province 430072, China.

This paper is the second of two focusing on the analytical solutions for light transport in infinite homogeneous tissue with an azimuth-dependent (m-dependent) anisotropic scattering kernel by two approaches, Case's singular eigenfuncions (CSEs) expansion and Fourier transform, and proving the consistence of the two solutions theoretically. In this paper, the analytical solution for the m-dependent truncated scattering kernel was derived via the Fourier transform and inversion, and expanded with the m-dependent generalized singular eigenfuncions (GSEs). Two kinds of GSEs that are defined by Ganapol in the case are extended to arbitrary azimuthal orders and proven to be consistent with CSEs both in expression forms and in intrinsic behaviors. By applying the Fourier transform inversion on the solution for the three-term recurrences, the Green's function of radiance distributions is obtained successfully, and it conforms perfectly to the CSEs solution in the limit, which has already been discussed in our first accompanying paper. Meanwhile, as a byproduct, a series of identities about the m-dependent Chandrasekhar orthogonal polynomials were presented and will be greatly helpful for further studies.
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http://dx.doi.org/10.1364/BOE.9.004031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157762PMC
September 2018

Thermal tomography for monitoring tumor response to neoadjuvant chemotherapy in women with locally advanced breast cancer.

Oncotarget 2017 Sep 25;8(40):68974-68983. Epub 2017 Mar 25.

Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, P. R. China.

Background & Aims: This study aims to analyze the feasibility and predictive value of thermal tomography (TT) for monitoring early treatment response in patients with locally advanced breast cancer (LABC) receiving neoadjuvant chemotherapy (NAC).

Methods: Patients with LABC who were due to receive six cycles of NAC were examined by TT prior to NAC, the second cycle of NAC, the fourth cycle of NAC and surgery. Changes in TT parameters and ultrasonography were correlated with pathologic response to NAC, and the predictive value was assessed.

Results: Forty-four patients were evaluable for response (25 pathologic responders and 19 nonresponders). As early as after the first cycle of NAC, changes in the TT parameters ΔTs, ΔTn, and ΔTa correlated significantly with pathologic response ( < 0.05). The best predictor of pathologic response after the 6th cycle of NAC was TT (area under the receiver operating characteristic curve, 0.794), as opposed to cross-sectional areas and the longest diameter by ultrasonography.

Conclusions: TT allows for monitoring early tumor response to NAC and can predict pathologic response in the early stages of therapy. Therefore, TT could be used as a novel imaging modality to monitor NAC treatment.
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http://dx.doi.org/10.18632/oncotarget.16569DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5620312PMC
September 2017

Tracing methamphetamine and amphetamine sources in wastewater and receiving waters via concentration and enantiomeric profiling.

Sci Total Environ 2017 Dec 25;601-602:159-166. Epub 2017 May 25.

Laboratory of Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, 100871 Beijing, PR China. Electronic address:

Wastewater analysis is a promising approach to monitor illicit drug abuse of a community. However, drug use estimation via wastewater analysis may be biased by sources other than abuse. This is especially true for methamphetamine and amphetamine as their presence in wastewater may come from many sources, such as direct disposal or excretion following administration of prescription drugs. Here we traced methamphetamine and amphetamine sources via concentration and enantiomeric profiling of the two compounds from black market to receiving waters. Methamphetamine in wastewater was found to predominantly arise from abuse, proving the feasibility of using wastewater analysis for estimating its consumption in China. Amphetamine abuse was previously considered negligible in East and Southeast Asia. However, we found that amphetamine was abused considerably (up to 90.7mg/1000inh/day) in a significant number (>20%) of major cities in China. Combined concentration and enantiomeric profiling also revealed direct disposal into receiving waters of methamphetamine manufactured by different processes. These findings have important implications for monitoring of and law enforcement against methamphetamine/amphetamine abuse and related crimes in China and abroad.
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http://dx.doi.org/10.1016/j.scitotenv.2017.05.045DOI Listing
December 2017

[Research on Three-dimensional Temperature Field Reconstruction in Biological Tissue Based on Multi-island Genetic Algorithm].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2016 Aug;33(4):666-73

The nondestructive reconstruction of three-dimensional(3D)temperature field in biological tissue is always an important problem to be resolved in biomedical engineering field.This paper presents a novel method of nondestructive reconstruction of 3Dtemperature field in biological tissue based on multi-island genetic algorithm(MIGA).By this method,the resolving of inverse problem of bio-heat transfer is transformed to be a solving process of direct problem.An experiment and its corresponding simulation were carried out to verify the feasibility and reliability.In the experiment a high purity polypropylene material,whose thermophysical parameters were similar to the fat tissue being tested,were adopted so that it could avoid the negative results created by the other factors.We set the position P(x,y,z)as the point heat source in the biological tissue and its temperature t as optimization variable,got the experimental temperature values of the points in a module surface,subtracted them from the corresponding simulating temperature values in the same module surface,and then took the sum of absolute value.We took it as the objective function of successive iteration.It was found that the less the target value was,the more optimal the current variables,i.e.the heat source position and the temperature values,were.To improve the optimization efficiency,a novel establishment method of objective function was also provided.The simulating position and experimental position of heat source were very approximate to each other.When the optimum values are determined,the corresponding 3D temperature field is also confirmed,and the temperature distribution of arbitrary section can be acquired.The MIGA can be well applied in the reconstruction of 3Dtemperature field in biological tissue.Because of the differences between the MIGA and the traditional numerical methods,we do not have to acquire all the data of surface.It is convenient and fast,and shows a prosperous application future.
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August 2016

Occurrence of illicit drugs in surface waters in China.

Environ Pollut 2016 Jun 1;213:395-402. Epub 2016 Mar 1.

Laboratory of Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing, 100871, PR China. Electronic address:

Illicit drugs have been recognized as a group of emerging contaminants. In this work, occurrence of common illicit drugs and their metabolites in Chinese surface waters was examined by collecting samples from 49 lakes and 4 major rivers across the country. Among the drugs examined, methamphetamine and ketamine were detected with highest frequencies and concentration levels, consistent with the fact that these are primary drugs of abuse in China. Detection frequencies and concentrations of other drugs were much lower than in European lakes and rivers reported in the literature. In most Chinese surface waters methamphetamine and ketamine were detected at concentrations of several ng L(-1) or less, but in some southern lakes and rivers, these two drugs were detected at much higher concentrations (up to several tens ng L(-1)). Greater occurrence of methamphetamine and ketamine in southern surface waters was attributed to greater abuse and more clandestine production of the two drugs in southern China.
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http://dx.doi.org/10.1016/j.envpol.2016.02.036DOI Listing
June 2016

Porous biphasic calcium phosphate ceramics coated with nano-hydroxyapatite and seeded with mesenchymal stem cells for reconstruction of radius segmental defects in rabbits.

J Mater Sci Mater Med 2015 Nov 8;26(11):257. Epub 2015 Oct 8.

Department of Sports Medicine, Research Center of Sports Medicine, Xiangya Hospital, Central South University, Changsha, People's Republic of China.

The osteoconduction of porous biphasic calcium phosphate (BCP) ceramics has been widely reported. In a previous study, we demonstrated that applying a nano-hydroxyapatite (nHA) coating enhances the osteoinductive potential of BCP ceramics, making these scaffolds more suitable for bone tissue engineering applications. The aim of the present study was to determine the effects of reconstructing radius defects in rabbits using nHA-coated BCP ceramics seeded with mesenchymal stem cells (MSCs) and to compare the bone regeneration induced by different scaffolds. Radius defects were created in 20 New Zealand rabbits, which were divided into four groups by treatment: porous BCP ceramics (Group A), nHA-coated porous BCP ceramics (Group B), porous BCP ceramics seeded with rabbit MSCs (Group C), and nHA-coated porous BCP ceramics seeded with rabbit MSCs (Group D). After in vitro incubation, the cell/scaffold complexes were implanted into the defects. Twelve weeks after implantation, the specimens were examined macroscopically and histologically. Both the nHA coating and seeding with MSCs enhanced the formation of new bone tissue in the BCP ceramics, though the osteoinductive potential of the scaffolds with MSCs was greater than that of the nHA-coated scaffolds. Notably, the combination of nHA coating and MSCs significantly improved the bone regeneration capability of the BCP ceramics. Thus, MSCs seeded into porous BCP ceramics coated with nHA may be an effective bone substitute to reconstruct bone defects in the clinic.
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http://dx.doi.org/10.1007/s10856-015-5590-4DOI Listing
November 2015

Synthesis of Ti-Ta alloys with dual structure by incomplete diffusion between elemental powders.

J Mech Behav Biomed Mater 2015 Nov 31;51:302-12. Epub 2015 Jul 31.

State Key Laboratory of Porous Metal Materials, Northwestern Institute of Nonferrous Metals Research, Xi'an 7100012, PR China.

In this work, powder metallurgical (PM) Ti-Ta alloys were sintered using blended elemental powders. A dual structure, consisting of Ti-rich and Ta-rich zones, was formed due to the insufficient diffusion between Ti and Ta powders. The microstructure, mechanical properties and in vitro biological properties of the alloys were studied. Results indicated that the alloys have inhomogenous microstructures and compositions, but the grain structures were continuous from the Ti-rich zone to the Ta-rich zone. The Ta-rich zone exhibited a much finer grain size than the Ti-rich zone. The alloys had a high relative density in the range of 95-98%, with the porosity increasing with the content of Ta due to the increased difficulty in sintering and the formation of Kirkendall pores. The alloys had a good combination of low elastic modulus and high tensile strength. The strength of alloys was almost doubled compared to that of the ingot metallurgy alloys with the same compositions. The low elastic modulus was due to the residual pores and the alloying effect of Ta, while the high tensile strength resulted from the strengthening effects of solid solution, fine grain size and α phase. The alloys had a high biocompatibility due to the addition of Ta, and were suitable for the attachment of cells due to the surface porosity. It was also indicated that PM Ti-(20-30)Ta alloys are promising for biomedical applications after the evaluations of both the mechanical and the biological properties.
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http://dx.doi.org/10.1016/j.jmbbm.2015.07.004DOI Listing
November 2015

Powder metallurgical low-modulus Ti-Mg alloys for biomedical applications.

Mater Sci Eng C Mater Biol Appl 2015 Nov 12;56:241-50. Epub 2015 Jun 12.

State Key Laboratory of Porous Metal Materials, Northwestern Institute of Nonferrous Metals Research, Xi'an 7100012, PR China.

In this work, powder metallurgical (PM) Ti-Mg alloys were prepared using combined techniques of mechanical alloying and spark plasma sintering. The alloys mainly consist of super saturations of Mg in Ti matrix, and some laminar structured Ti- and Mg-rich phases. The PM Ti-Mg alloys contain a homogeneous mixtures of nanocrystalline Mg and Ti phases. The novel microstructures result in unconventional mechanical and biological properties. It has been shown that the PM Ti-Mg alloys have a much lower compression modulus (36-50GPa) compared to other Ti alloys, but still remain a very high compressive strength (1500-1800MPa). In addition, the PM Ti-Mg alloys show good biocompatibility and bioactivity. Mg can dissolve in the simulated body fluids, and induce the formation of the calcium phosphate layer. The compression modulus of PM Ti-Mg alloys decreases with the amount of Mg, while the bioactivity increases. Although the corrosion resistance of Ti-Mg alloys decreases with the content of Mg, the alloys still show good stability in simulated body fluid under electrochemical conditions. The indirect and direct cytotoxicity results show that PM Ti-Mg alloys have a good biocompatibility to NIH-3T3 cells. Therefore, the PM Ti-Mg alloys are promising candidates in biomedical applications.
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http://dx.doi.org/10.1016/j.msec.2015.06.010DOI Listing
November 2015

Methamphetamine and ketamine use in major Chinese cities, a nationwide reconnaissance through sewage-based epidemiology.

Water Res 2015 Nov 17;84:76-84. Epub 2015 Jul 17.

Laboratory of Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, 100871 Beijing, China. Electronic address:

Sewage-based epidemiology was applied to examine geographic variations in methamphetamine (METH) and ketamine (KET) use in China. Influent and effluent wastewater samples were collected from 36 sewage treatment plants (STPs) in 18 major cities that cover all the geographic regions of the country. Mean METH loads of the cities ranged from 12.5 ± 14.9 to 181.2 ± 6.5 mg/1000 inh/d, whereas mean KET loads ranged from <0.2 to 89.6 ± 27.4 mg/1000 inh/d. No clear geographical pattern was observed in METH use, although slightly lower use in north and east China relative to other regions can be suggested. In contrast, an overall increasing trend from the north to the south was evident for KET loads. Apparent METH removal was greater than 80% at most STPs, whereas KET removal was less than 50% at most STPs and was even negative (i.e., measured effluent concentrations were greater than influent concentrations) at a significant number of STPs. Results in Beijing, Shanghai, and Shenzhen indicate that METH use in China may have increased substantially since 2012, whereas KET use did not significantly change, if not decreased. Comparison between seizures and estimated consumptions reveals that seizures in most Chinese provinces are far less than consumptions. In several provinces (e.g., Guangdong and Yunan), however, seizures were found to exceed consumptions, indicating that a significant fraction of METH and KET seized in these provinces is destined for consumption in other places.
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http://dx.doi.org/10.1016/j.watres.2015.07.025DOI Listing
November 2015

Next-Generation Sequencing Techniques Reveal that Genomic Imprinting Is Absent in Day-Old Gallus gallus domesticus Brains.

PLoS One 2015 10;10(7):e0132345. Epub 2015 Jul 10.

Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.

Genomic imprinting is a phenomenon characterized by parent-of-origin-specific gene expression. While widely documented in viviparous mammals and plants, imprinting in oviparous birds remains controversial. Because genomic imprinting is temporal- and tissue-specific, we investigated this phenomenon only in the brain tissues of 1-day-old chickens (Gallus gallus). We used next-generation sequencing technology to compare four transcriptomes pooled from 11 chickens, generated from reciprocally crossed families, to the DNA sequences of their parents. Candidate imprinted genes were then selected from these sequence alignments and subjected to verification experiments that excluded all but one SNP. Subsequent experiments performed with two new sets of reciprocally crossed families resulted in the exclusion of that candidate SNP as well. Attempts to find evidence of genomic imprinting from long non-coding RNAs yielded negative results. We therefore conclude that genomic imprinting is absent in the brains of 1-day-old chickens. However, due to the temporal and tissue specificity of imprinting, our results cannot be extended to all growth stages and tissue types.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0132345PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498732PMC
April 2016

Diagnosis of breast tumor using thermal tomography q-r curve.

J Biomed Opt 2015 Jun;20(6):068001

Wuhan University, School of Physics and Technology, Department of Electronic Science and Technology, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province 430072, China.

Metabolic heat, the product following the metabolism of cells, is closely related to the pathological information of living organisms, which means there are strong connections between the heat distribution and the pathological state of the living organism. The mathematical function δ is introduced in the classical Pennes bioheat transfer equation as a point heat source, and by simplifying the boundary condition, a bioheat transfer model is established. Based on the temperature distribution of the human body surface, the q−r curve of heat intensity q varying with depth r is acquired while combining the fitting method of the Lorentz curve. According to 34,977 clinical confirmed cases and the corresponding classified statistics, diagnostic criteria (for breast diseases) for judging diseases by the q−r curve are proposed. The P -value of our statistics is <0.05 , which means our classified statistics are reliable. Six typical clinical examinations are performed, and the diagnosis results are very consistent with those of B-ultrasonic images, molybdenum target x-ray, and pathological examination, which suggests that the method of diagnosing diseases with a q−r curve has very good prospects for application. It is radiation free and noninvasive to the human body.
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http://dx.doi.org/10.1117/1.JBO.20.6.068001DOI Listing
June 2015

Facile and controllable synthesis of hydroxyapatite/graphene hybrid materials with enhanced sensing performance towards ammonia.

Analyst 2015 Aug;140(15):5235-42

State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, PR China.

In this work, needle-like and micro-spherical agglomerates of nanocrystalline hydroxyapatite (HA) were successfully assembled on the surface of graphene sheets with the aid of dopamine having two roles, as a template and a reductant for graphite oxide during the process of self-polymerization. The crystalline structure and micromorphology of HA can be conveniently regulated by controlling the mineralization route either with a precipitation (cHA/GR) or biomimetic methodology (bHA/GR). Both the composites exhibit improvements of ∼150% and ∼250% in sensitivity towards the sensing of ammonia at room temperature, compared with that of bare graphene. The combination of the multi-adsorption capability of HA and the electric conductivity of graphene is proposed to be the major reason for the observed enhancements. Gas sensing tests demonstrated that the HA/GR composites exhibit excellent selectivity, high sensitivity and repeatable stability towards the analytical sensing of ammonia.
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http://dx.doi.org/10.1039/c5an00622hDOI Listing
August 2015

Q-r curve of thermal tomography and its clinical application on breast tumor diagnosis.

Biomed Opt Express 2015 Apr 3;6(4):1109-23. Epub 2015 Mar 3.

Department of Electronic Science and Technology, School of Physics and Technology, Wuhan University, Wuhan 430072, China.

Heat is the product following the metabolism of cells, and the metabolism is closely related with the pathological information of living organism. So, there are strong ties between the heat distribution and the pathological state in living organism. In this paper, the mathematical function δ is introduced in the classical Pennes bio-heat transfer equation as the point heat source. By simplifying the boundary conditions, a novel bio-heat transfer model is established and solved in a spherical coordinate system. Based on the temperature distribution of human body surface, the information of heat source is mined layer by layer, and the corresponding q-r curve of heat intensity varying with depth is acquired combining the fitting method of Lorentz curve. According to a large number of clinical confirmed cases and statistics, the diagnostic criteria judging diseases by q-r curve are proposed. Five typical clinical practices are performed and four of the diagnosis results are very consistent with those of molybdenum target (MT) X-ray, B-ultrasonic images and pathological examination, one gives the result of early stage malignant tumor that MT X-ray and B-ultrasonic can't check out. It is a radiation-free green method with noninvasive diagnostic procedure and accurate diagnosis result.
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http://dx.doi.org/10.1364/BOE.6.001109DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4399653PMC
April 2015

Temporal expression and DNA hypomethylation profile of CD30 in Marek's disease virus-infected chicken spleens.

Poult Sci 2015 Jun 3;94(6):1165-9. Epub 2015 Apr 3.

Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

Marek's disease (MD) is a viral neoplastic disease of chickens caused by Marek's disease virus (MDV), which is serious threat to worldwide poultry industry. Our previous studies showed that the CD30 gene was hypomethylated in MD lymphoma. In this study, we further analyzed differential expression patterns and methylation levels of the CD30 gene between MDV-infected and noninfected spleens at 4, 7, 14, 21, and 28 d postinfection (dpi). The results showed that the expression of CD30 in MDV-infected spleens was significantly lower than that in noninfected spleens at 4 dpi. The expression of CD30 did not present significant difference between MDV-infected and noninfected spleens at 7 and 14 dpi. However, an increased expression of CD30 was presented in MDV-infected spleens at both 21 and 28 dpi. Simultaneously, CD30 showed a lower DNA methylation level in MDV-infected spleens at 14, 21, and 28 dpi. The results indicated that CD30 gene was involved in the whole process of MD tumorigenesis and upregulated expression of CD30 in MDV-infected spleens might be attributed to the hypomethylation of promoter of CD30 gene.
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http://dx.doi.org/10.3382/ps/pev100DOI Listing
June 2015

Effects of different rearing systems on meat production traits and meat fiber microstructure of Beijing-you chicken.

Anim Sci J 2015 Jul 11;86(7):729-35. Epub 2014 Dec 11.

Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.

Beijing-you is a Chinese local chicken which is raised for both meat and eggs. In the present study, we detected the effects of different rearing systems on growth, slaughtering performances and meat quality of Beijing-you chickens at 26-40 weeks of age. Six hundred Beijing-you hens were randomly allocated into two groups at 16 weeks of age and raised in free range or battery cage systems. The body weight, slaughtering performance and meat quality were measured for each group at the ages of 26, 30, 35 and 40 weeks. Some of the traits were dramatically influenced by the two systems, although most of them did not show significant changes. For the meat fiber microstructure, we found that the diameter of thigh and breast muscle fiber in the free range group were significantly increased than in the cage group (P < 0.05) at 26 weeks of age. The ratio of fast muscle fiber in thigh muscle samples of the free range group was significantly reduced compared to that of cage group at both 35 (P < 0.01) and 40 (P < 0.01) weeks of age, indicating that the free range system could promote the transforming of fast muscle fiber to slow muscle fiber.
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http://dx.doi.org/10.1111/asj.12347DOI Listing
July 2015

A simple and efficient method for breast cancer diagnosis based on infrared thermal imaging.

Cell Biochem Biophys 2015 Jan;71(1):491-8

Key Laboratory of Artificial Micro & Nano-structures of Ministry of Education, Department of Electronic Science and Technology, School of Physics and Technology, Wuhan University, Wuhan, 430072, China.

This study aims to evaluate the feasibility and efficacy of quantitative diagnosis through thermal analysis of abnormal metabolism. In this paper, an analytical-based steady-state solution for the thermal inverse problem was developed, considering an equivalent point heat source embedded in the tissue. Based on this solution, we developed a simple and efficient algorithm that generates solutions for the nonlinear heat conduction model. Using the nonlinear fitting analysis, a regular distribution can be derived from the raw thermal patterns of the skin surface above the tumor, and the power and depth of the equivalent heat source can be derived to investigate whether the tumor is malignant or benign. The thermal power Q of internal heat source was estimated to predict the satisfactory approaches to distinguish between benign and malignant tumors. The results of four clinical cases (female patients with malignant tumor and benign tumor) show that the estimated values of the power of the heat sources in malignant cases (fatty: Q = 0.34851 W; dense: Q = 0.46933 W) are both far greater than the ones in benign (fatty: Q = 0.04721 W; dense: Q = 0.07717 W), irregardless of the breast density. The correlation coefficients (R (2)) of the nonlinear curve fittings are all above 0.98. The new thermal method proposed in this study would help to improve the preciseness of diagnosis on breast masses (malignant or benign).
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http://dx.doi.org/10.1007/s12013-014-0229-5DOI Listing
January 2015

Green electrochemical sensing platforms: utilizing hydroxyapatite derived from natural fish scales as a novel electrochemical material for the sensitive detection of kidney injury molecule 1 (KIM-1).

Analyst 2014 Nov;139(21):5362-6

National Engineering Research Center for Nanomedicine, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, P.R. China.

Urinary KIM-1 is an ideal biomarker for acute kidney injury diagnosis. The proof-of-concept is demonstrated by utilizing the hydroxyapatite derived from natural fish scales as an electrode material, where the sensing of KIM-1 is shown to be possible for the first time with a linear range from 0.01 to 0.20 μg mL(-1) and a detection limit of 0.017 μg mL(-1) under model conditions; proof-of-concept is demonstrated in spiked urine.
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http://dx.doi.org/10.1039/c4an00957fDOI Listing
November 2014

Estimation of amphetamine and methamphetamine uses in Beijing through sewage-based analysis.

Sci Total Environ 2014 Aug 4;490:724-32. Epub 2014 Jun 4.

Laboratory of Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, 100871 Beijing, PR China. Electronic address:

Sewage epidemiology has been proven as an effective approach to estimate the use of illicit drugs by a population. In this study, sewage analysis was applied to examine the patterns of amphetamine (AMP) and methamphetamine (METH) uses in the urban area of Beijing. Influent and effluent samples were collected from all the thirteen sewage treatment plants (STPs) in the urban area during two sampling campaigns. METH concentrations in influents were found to range from several tens to several hundred ng·L(-1), whereas AMP concentrations ranged from several to several tens ng·L(-1). The concentration ratios between AMP and METH in influents at most STPs were close to the rate of AMP excretion following METH ingestion, indicating that AMP in sewage in Beijing was predominately from the metabolism of METH. Much higher METH loads were observed in the center part of the urban area in Beijing, indicating a strong correlation between METH use and economic level and entertainment activities. Seasonal variation in METH loads was significant, with greater use in summer than in winter. Significant difference in METH loads between weekdays and weekend days were observed in winter but not in summer. No clear trend in diurnal variation of METH use was observed. Nearly complete removal of METH occurred at the STPS in Beijing. Apparent removal rates of AMP were lower than those of METH, likely due to degradation of METH into AMP during the wastewater treatment processes. In summary, this study represents the first application of sewage epidemiology to the entire urban population of a metropolitan in mainland China and provided an overview of METH and AMP uses in the city.
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http://dx.doi.org/10.1016/j.scitotenv.2014.05.042DOI Listing
August 2014

Application of a sewage-based approach to assess the use of ten illicit drugs in four Chinese megacities.

Sci Total Environ 2014 Jul 31;487:710-21. Epub 2014 Jan 31.

Toxicological Centre, University of Antwerp, Antwerp, Belgium. Electronic address:

Sewage-based epidemiology was applied for the first time to a number of mainland Chinese megacities. The application monitored influents to 9 sewage treatment plants (STPs) to estimate the use of illicit drugs in Beijing, Guangzhou, Shenzhen, and Shanghai. Altogether, 11.4 million inhabitants were covered during September-October 2012. 24-h composite raw sewage samples were collected for 4 consecutive days at each STP. Each collected sample was analyzed for cocaine, benzoylecgonine, ecgonine methylester, methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine, 6-monoacetylmorphine, amphetamine, methamphetamine, ecstasy, mephedrone, methylenedioxypyrovalerone, 11-nor-9-carboxy-delta-9-tetrahydrocannabinol, ketamine, and norketamine. Through the analysis of these chemical residues, the use of amphetamine, cannabis, cocaine, ecstasy, heroin, mephedrone, methadone, methamphetamine, methylenedioxypyrovalerone and ketamine among Chinese urban inhabitants was monitored. The results obtained demonstrated in a quantitative way that the drug use patterns of Chinese are different from their European counterparts. Abuse of methamphetamine and ketamine was particularly noteworthy in China, while consumption of cocaine and ecstasy, the most popular drugs in Europe, was very low among the sampled Chinese inhabitants. Further, the use of most drugs demonstrated a geographical trend, since their use was much higher in the southern cities of Shenzhen and Guangzhou than it was in Beijing and Shanghai. Interestingly, the exclusive, but minor, metabolite of heroin, 6-monoacetylmorphine, was detected only sporadically. This would suggest that the use of heroin among Chinese urban users sampled in the study was low. Further, the patterns of drug use observed during the study are largely consistent with trends reported by the United Nations Office on Drugs and Crime. Overall, our study suggests that sewage-based epidemiology can readily be used to monitor the use of illicit drugs in those countries/regions where traditional means to monitor drug use patterns have only yielded limited or information of questionable reliability.
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http://dx.doi.org/10.1016/j.scitotenv.2014.01.043DOI Listing
July 2014

Rose-bengal-conjugated gold nanorods for in vivo photodynamic and photothermal oral cancer therapies.

Biomaterials 2014 Feb 9;35(6):1954-66. Epub 2013 Dec 9.

Department of Physics and Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China. Electronic address:

Gold nanorods (GNRs) conjugated with rose bengal (RB) molecules exhibit efficient singlet oxygen generation when illuminated by 532 nm green light and high photothermal efficiency under 810 nm near-infrared (NIR) irradiation. In vitro experiments show that reactive oxygen species generated by green light and hyperthermia produced by NIR light constitute two different mechanisms for cancer cell death. The RB-GNRs also exhibit improved photodynamic efficacy by enhancing the uptake of RB by cancer cells. In vivo experiments are conducted on hamster cheek pouches to resemble the human oral cancer conditions more accurately to assess the therapeutic effectiveness. Compared to the single photodynamic therapy (PDT) or photothermal therapy (PTT), the RB-GNRs with combined PDT-PTT capabilities provide better therapeutic effects against oral cancer and have large potential in cancer treatment.
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http://dx.doi.org/10.1016/j.biomaterials.2013.11.066DOI Listing
February 2014

Removal of ZnO nanoparticles in simulated wastewater treatment processes and its effects on COD and NH(4)(+)-N reduction.

Water Sci Technol 2013 ;67(2):254-60

Laboratory of Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing, 100871, China.

For many engineered nanoparticles, the primary pathway of release into the environment is via sewage and industrial wastewater discharges. In this work, the removal of uncoated ZnO nanoparticles (ZnO NPs) during simulated wastewater treatment processes and its impact on treatment performance were examined. Simulated primary clarification removed the majority (about 70%) of the dosed ZnO NPs. During simulated sequencing batch reactor (SBR) processes, ZnO NPs were completely removed in each cycle throughout the 11-day experimental duration (two cycles per day). Continuous input of ZnO NPs into the wastewater (at concentrations up to 5 mg L(-1)) did not reduce chemical oxygen demand (COD) removal. NH(4)(+)-N removal was reduced at a dosing concentration of 5 mg L(-1) ZnO NPs per cycle. Inhibition of respiration of nitrifying microorganisms by ZnO NPs corroborated the reduction of NH(4)(+)-N removal. These results indicate that if the wastewater is treated, the release of ZnO NPs into receiving water bodies would be minimal and ZnO NPs would mainly accumulate in biosolids. Uncoated ZnO NPs in wastewater at very high concentrations may have some adverse effects on activated sludge process.
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http://dx.doi.org/10.2166/wst.2012.530DOI Listing
March 2013

Removal of silver nanoparticles in simulated wastewater treatment processes and its impact on COD and NH(4) reduction.

Chemosphere 2012 Apr 14;87(3):248-52. Epub 2012 Jan 14.

Laboratory of Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing 100871, PR China.

The increasing utilization of silver nanoparticles in industrial and consumer products has raised concern to wastewater treatment utilities due to its antimicrobial activity. In this work, the removal of citrate stabilized silver nanoparticles (Ag-NPs) during the wastewater treatment processes and its impact on treatment performance were examined. During simulated primary clarification, over 90% of the Ag-NPs remained in the wastewater, indicating that the majority of silver nanoparticles in sewage would enter the subsequent treatment units. During sequencing batch reactor processes, silver nanoparticles were effectively removed in each cycle throughout the 15-d experimental duration. Continuous input of silver nanoparticles into the wastewater did not significantly alter chemical oxygen demand (COD) removal. NH(4) removal was reduced at the beginning of the SBR experiment but quickly recovered at the later stage of the experiment. This study demonstrated that in the near future it is unlikely that citrate-stabilized Ag-NPs released into sewage will cause significant adversary effects on the COD and NH(4) removal of activated sludge processes in municipal wastewater treatment plants.
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http://dx.doi.org/10.1016/j.chemosphere.2011.12.042DOI Listing
April 2012

Accumulation dynamics of chlordanes and their enantiomers in cockerels (Gallus gallus) after oral exposure.

Environ Sci Technol 2011 Sep 29;45(18):7928-35. Epub 2011 Aug 29.

Laboratory for Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing 100871, China.

After a single oral exposure of technical chlordane, levels of cis-chlordane (CC), trans-chlordane (TC), heptachlor (HEP), heptachlorepoxide (HEPX), and oxychlordane (OXY) were determined in gastrointestinal residues, droppings, and various tissues of cockerels at times of 60, 120, 160, 200, 300, 500, 1000, and 2000 min. Over 98% of CC and TC were found to be bioaccessible; only 1.1% of CC and TC were directly excreted through droppings without further biotransformation. According to the single-compartment toxicokinetic modeling, CC and TC shared similar absorption rates in the whole body while TC showed a slightly more rapid elimination rate, with a half-life of 13.4 h for CC and 12.5 h for TC. The metabolites HEPX and OXY appeared quickly in tissues 60 min after exposure and were mainly accumulated in fat and liver tissues. Concentrations of CC, TC, and HEP in cockerel tissues roughly followed the order as fat > intestine > skin > liver> brain > muscle > blood. Levels of CC, TC, and HEP in various tissues showed significant correlation with the lipid contents of the tissues (p < 0.05) for samples beginning 500 min after exposure. A multicompartment toxicokinetic model was developed to characterize the accumulation dynamics of CC and TC in the various tissues. All tissues of cockerels enantioselectively accumulated (-)-CC and (+)-TC, and fat, skin, and liver tissues showed a relatively stronger capacity of enantioenrichment. The enantiomer fractions (EFs) of droppings remained nearly racemic at first but gradually decreased to less than 0.5 for CC and increased to more than 0.5 for TC, which could rule out enantioselective absorption and excretion of CC and TC in cockerels. The one-compartment toxicokinetic model was applied to the individual enantiomers of CC and TC. Different elimination rates but similar absorption rates were observed between the enantiomers for both CC and TC.
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http://dx.doi.org/10.1021/es201629pDOI Listing
September 2011

[Development and validation of near-infrared brain blood-oxygen monitor].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2007 Dec;24(6):1220-3

Laboratory of Biomedical Physics, College of Physics Science & Technology, Wuhan University, Wuhan 430072, China.

The main purpose of this paper is to describe the design and development of the near-infrared blood-oxygen monitor which is based on the theory of detecting the blood-oxygen parameters of tissue by near-infrared, and the monitor can detect the blood-oxygen parameters of two sides of the local brain tissue. The monitor uses two wavelength ultra-high light LED as lamp-house, and two sensors detect the light scattered by two sides of the local brain tissue, which make the monitor achieve the function of dual detector. The appearance of the monitor's detector is designed to reduce effectively the noise brought by the background and alleviate the discomfort feelings of the patient. At the same time, this apparatus can monitor continuously the brain blood-oxygen parameters of the patients in real time, and output the curves of the blood-oxygen and oxygen concentration of two sides of brain tissue. At last, the biological model experiments and the tests in clinical setting validate the monitor.
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December 2007

Immunofluorescence detection with quantum dot bioconjugates for hepatoma in vivo.

J Biomed Opt 2007 Jan-Feb;12(1):014008

Wuhan University, Key Laboratory of Acoustic and Photonic Materials and Devices of Ministry of Education, Department of Physics, Wuhan 430072, China.

The use of highly specific and highly sensitive immunofluorescent probes is a promising approach for biomedical imaging in living tissue. We focus on immunofluorescence with quantum dot bioconjugates for hepatoma detection in vivo. We synthesized specific immunofluorescent probes by linking quantum dots to AFP (alpha-fetoprotein) antibody for specific binding AFP-an important marker for hepatocellular carcinoma cell lines. In in vivo studies, the characteristic quantum dot (QD) fluorescent property is exhibited by the QDs-Anti-AFP probes in tumor and they demonstrate active tumor targeting and spectroscopic hepatoma imaging with an integrated fluorescence imaging system. We investigate the inhomogeneous distribution of the QDs-Anti-AFP probes in tumor by using a site-by-site measurement method to test their ability for distribution studies of cancer cells. These results demonstrate the practicality of QD bioconjugates as attractive fluorescent probes for biomedical detection.
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http://dx.doi.org/10.1117/1.2437744DOI Listing
May 2007