Publications by authors named "Kaili Yang"

53 Publications

A multifunctional Fenton nanoagent for microenvironment-selective anti-biofilm and anti-inflammatory therapy.

Mater Horiz 2021 Apr 15;8(4):1264-1271. Epub 2021 Feb 15.

Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM) & Jiangsu National Synergetic Innovation Centre for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing 210023, China.

Bacterial biofilm infections are intractable to traditional antibiotic treatment and usually cause persistent inflammation. Chemodynamic therapy (CDT) based on the Fenton reaction has recently emerged as a promising anti-biofilm strategy. However, the therapeutic efficacy of current Fenton agents often suffers from inefficient Fenton activity and lacks anti-inflammatory capability. Herein, FePS nanosheets (NSs) are explored for the first time as novel microenvironment-selective therapeutic nanoagents for bacterial biofilm infections with both self-enhanced Fenton activity for an anti-biofilm effect and reactive oxygen species (ROS) scavenging properties for an anti-inflammatory effect. In biofilms with acidic microenvironments, FePS NSs release Fe to generate toxic ROS by Fenton reaction and reductive [PS] to enhance the Fenton activity by reducing Fe to Fe. In the surrounding normal tissues with neutral pH, FePS NSs scavenge ROS by reductive [PS] with an anti-inflammatory effect. This work demonstrates multifunctional Fenton nanoagents with microenvironment-selective ROS generation and elimination properties for effective treatment of bacterial biofilm infections with both anti-biofilm and anti-inflammatory effects.
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http://dx.doi.org/10.1039/d0mh01921fDOI Listing
April 2021

Mitochondria-Targeting MoS-Based Nanoagents for Enhanced NIR-II Photothermal-Chemodynamic Synergistic Oncotherapy.

ACS Appl Mater Interfaces 2021 Nov 17. Epub 2021 Nov 17.

Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Centre for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.

The synergy of chemodynamic therapy (CDT) and photothermal therapy (PTT) can improve anticancer efficacy, while the limited diffusion distance and the short lifetime of OH still greatly restrict the therapeutic efficacy of PTT-CDT. Herein, [email protected]@PEG/TPP (MPFPT) nanosheets (NSs) with mitochondria-targeting ability were reported for enhanced PTT-CDT synergistic oncotherapy. MPFPT NSs were prepared by covalent modification of poly(ethylene glycol) (PEG) and triphenylphosphonium (TPP) on polydopamine (PDA)-Fecoated MoS NSs. Co-localization experiments showed that MPFPT NSs can efficiently target mitochondria via the direction of TPP. Moreover, MPFPT NSs have good photothermal performance in the second near-infrared (NIR-II) region and can greatly accelerate the Fenton reaction from HO to generate more hydroxyl radicals (OH). In vitro experimental results showed that MPFPT NSs have improved therapeutic efficacy to cancer cells than similar MoS-based nanoagents without mitochondria-targeting units, which can be attributed to the short distance between mitochondria and MPFPT NSs and the efficient damage of mitochondria by in situ generated OH. In the 4T1 tumor-bearing mice model, MPFPT NSs demonstrated significantly enhanced therapeutic efficacy by PTT-CDT, suggesting the superiority of the mitochondria-targeting strategy. This study reveals that mitochondria-targeting MPFPT NSs are promising nanoagents for oncotherapy.
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http://dx.doi.org/10.1021/acsami.1c18311DOI Listing
November 2021

Fluorescence and ratiometric photoacoustic imaging of endogenous furin activity peptide functionalized MoS nanosheets.

Biomater Sci 2021 Nov 16. Epub 2021 Nov 16.

Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Centre for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.

Furin is an important cellular endoprotease, which is expressed at high levels in various cancer cells. Accurate and real-time detection of endogenous furin with high sensitivity and selectivity is significant for the diagnosis of cancer. Herein an activatable nanoprobe ([email protected]/peptide, MPPF) with dual-mode near-infrared fluorescence (NIRF)/ratiometric photoacoustic (PA) imaging of endogenous furin activity has been developed. The MPPF nanoprobes were constructed by the covalent functionalization of polydopamine (PDA) coated MoS nanosheets (NSs) with Cy7-labeled furin substrate peptides. Upon cleavage of the peptides by furin, Cy7 molecules are released from MPPF nanoprobes and recover their fluorescence, realizing furin activity detection with the limit of detection (LOD) down to 3.73 × 10 U mL. Meanwhile, the ratio of the PA signal at 768 nm to that at 900 nm (PA/PA) decreases over time due to the destruction of fluorescence resonance energy transfer effect from Cy7 to MoS NSs and the rapid clearance of small Cy7 molecules from tissues. Thus, the simultaneous change in NIRF and ratiometric PA signals enables the imaging of endogenous furin activity in real time, and with high sensitivity, and high selectivity in both tumor cells and tumor-bearing mice.
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http://dx.doi.org/10.1039/d1bm01410bDOI Listing
November 2021

Effects of dietary tributyrin and physterol ester supplementation on growth performance, intestinal morphology, microbiota and metabolites in weaned piglets.

J Appl Microbiol 2021 Oct 27. Epub 2021 Oct 27.

College of Animal Science and Technology, Hunan Agricultural University, Hunan Co-Innovation of Animal Production Safety, Changsha, Hunan, China.

Aim: This study was conducted to investigate the effects of dietary tributyrin (TB) and physterol ester (PSE) supplementation on the growth performance and intestinal health of weaned piglets.

Methods And Results: Ninety-six piglets were randomly allocated to one of four groups, including a control group (basal diet), TB group (basal diet + 1500 g t  TB), PSE group (basal diet + 300 g t PSE) and TB + PSE group (basal diet + 1500 g t  TB + 300 g t PSE). All groups had eight replicates with three piglets per replicate. The experiment lasted for 28 days. The results showed that dietary TB supplementation increased (p < 0.05) average daily feed intake and average daily gain, as well as the acetate and butyrate concentration in ileum, and dietary PSE supplementation decreased (p < 0.05) the ratio of feed to gain (F/G) on days 1-14 of the trial. Dietary TB or PSE alone supplementation improved the ratio of villus height to crypt depth (VH/CD) and the expression level of Occludin in ileum. The linear discriminant analysis effect size analysis identified eight biomarkers in the control group, 18 in the TB + PSE group, two in the PSE group in ileum respectively. Correlation analysis showed that the relative abundances of Enterococcus, and Streptococcus were positively correlated (p < 0.05) with propionate concentration, while the relative abundance of Clostridium_sensu_stricto_1 was negatively correlated (p < 0.05) with acetate concentration in ileum.

Conclusion: These findings suggest that dietary TB or PSE alone supplementation could alter the growth performance, intestinal morphology, microbiota community and metabolites of weaned piglets.

Significance And Impact Of The Study: Weaning stress is a major cause of slow growth and increased diarrhoea in piglets. This study demonstrated that dietary TB and PSE presented a beneficial role in growth performance and gut health via regulating intestinal morphology, microbiota composition and metabolites.
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http://dx.doi.org/10.1111/jam.15321DOI Listing
October 2021

Real-world outcomes of regorafenib combined with immune checkpoint inhibitors in patients with advanced or metastatic microsatellite stable colorectal cancer: A multicenter study.

Cancer Immunol Immunother 2021 Oct 24. Epub 2021 Oct 24.

Department of Oncology, Beijing Hospital, National Center of Gerontology, Beijing, 100730, China.

Background: Treatment strategies are limited for patients with chemotherapy refractory microsatellite stable (MSS) colorectal cancer. We aim to evaluate the efficacy and safety of immune checkpoint inhibitors (ICIs) combined with regorafenib in this population in routine clinical practice.

Methods: We retrospectively analyzed patients with advanced or metastatic colorectal cancer who received at least one dose of ICIs combined with regorafenib in 14 Chinese medical centers. The primary outcome was objective response rate (ORR). This study was registered at ClinicalTrials.gov on February 2020 (NCT04771715).

Results: Eighty-four patients received ICIs combined with regorafenib from January 2019 to January 2021. Most patients (91%) received two or more systemic treatment lines before the study treatment. Seventy-six patients (90%) had confirmed MSS status. At a median follow-up of 5.5 months, four patients achieved partial response (5%) and 37 patients achieved stable disease (45%) as the best response. The median progression-free survival (PFS) was 3.1 months, and the median overall survival was 17.3 months. Eleven patients (13%) remained progression-free for more than 6 months. Baseline liver metastasis (HR 1.98, 95%CI 1.07-3.69, P = 0.03) and neutrophil-lymphocyte ratio (NLR) of ≥ 1.5 (HR 2.83, 95%CI 1.00-7.98, P = 0.05) were associated with shorter PFS in multivariate analysis. Grade 3 or higher treatment-related adverse events (TRAEs) occurred in 16 patients (19%).

Conclusion: The combination of ICIs with regorafenib can be a valuable treatment option for a proportion of patients with chemotherapy refractory MSS colorectal cancer. Patients with no liver metastasis and a low NLR at baseline may derive most benefit from this strategy.
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http://dx.doi.org/10.1007/s00262-021-03083-3DOI Listing
October 2021

Remote estimation of leaf area index (LAI) with unmanned aerial vehicle (UAV) imaging for different rice cultivars throughout the entire growing season.

Plant Methods 2021 Aug 10;17(1):88. Epub 2021 Aug 10.

School of Remote Sensing and Information Engineering, Wuhan University, Wuhan, China.

Background: Rice is one of the most important grain crops worldwide. The accurate and dynamic monitoring of Leaf Area Index (LAI) provides important information to evaluate rice growth and production.

Methods: This study explores a simple method to remotely estimate LAI with Unmanned Aerial Vehicle (UAV) imaging for a variety of rice cultivars throughout the entire growing season. Forty eight different rice cultivars were planted in the study site and field campaigns were conducted once a week. For each campaign, several widely used vegetation indices (VI) were calculated from canopy reflectance obtained by 12-band UAV images, canopy height was derived from UAV RGB images and LAI was destructively measured by plant sampling.

Results: The results showed the correlation of VI and LAI in rice throughout the entire growing season was weak, and for all tested indices there existed significant hysteresis of VI vs. LAI relationship between rice pre-heading and post-heading stages. The model based on the product of VI and canopy height could reduce such hysteresis and estimate rice LAI of the whole season with estimation errors under 24%, not requiring algorithm re-parameterization for different phenology stages.

Conclusions: The progressing phenology can affect VI vs. LAI relationship in crops, especially for rice having quite different canopy spectra and structure after its panicle exsertion. Thus the models solely using VI to estimate rice LAI are phenology-specific and have high uncertainties for post-heading stages. The model developed in this study combines both remotely sensed canopy height and VI information, considerably improving rice LAI estimation at both pre- and post-heading stages. This method can be easily and efficiently implemented in UAV platforms for various rice cultivars during the entire growing season with no rice phenology and cultivar pre-knowledge, which has great potential for assisting rice breeding and field management studies at a large scale.
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http://dx.doi.org/10.1186/s13007-021-00789-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353786PMC
August 2021

Identification of nitrate sources in the Jing River using dual stable isotopes, Northwest China.

Environ Sci Pollut Res Int 2021 Jul 17. Epub 2021 Jul 17.

State Key Laboratory of Loess and Quaternary Geology, Institute of Earth Environment, Chinese Academy of Sciences, Xi'an, 710061, China.

Nitrate (NO) contamination has become a dominant international problem in the aquatic environment, so identifying the sources and transformations of NO is the basis for improving water quality. Since the Jing River is the largest tributary of the Wei River, to understand its water quality, this study collected surface water samples from the Shaanxi section of the Jing River during the dry season. The potential sources of NO were analyzed by hydrochemical and bi-isotopic methods, and the SIAR model was used to estimate the proportional contribution of each source. Results indicated that NO-N was the main form of inorganic nitrogen in this area, and the average total nitrogen content was 10.23 mg·L, which showed that nitrogen pollution was highly serious; the transformation process of nitrogen in this study area was mainly nitrification; The results of Bayesian model showed that manure and sewage contributed to the most NO (64.39%) in the dry season, followed by soil nitrogen, which was 26.35%. These results help to adopt better nitrogen management measures to meet the national environmental quality standards for surface water.
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http://dx.doi.org/10.1007/s11356-021-15380-6DOI Listing
July 2021

Whole mitochondrial genome analysis in Chinese patients with keratoconus.

Mol Vis 2021 8;27:270-282. Epub 2021 May 8.

Henan Provincial People's Hospital, Henan Eye Hospital, Henan Eye Institute, People's Hospital of Zhengzhou University, Henan University People's Hospital, Zhengzhou, China.

Purpose: Keratoconus (KC) is a corneal disorder characterized by corneal ectasia, progressive corneal thinning, and conical protrusion. This study aimed to elucidate the mitochondrial gene profile in Chinese patients with KC, analyze the mitochondrial haplogroup and heteroplasmy, and further explore the association between mitochondrial genes and KC.

Methods: Mitochondrial sequencing was conducted on 100 patients with KC and 100 matched controls. Haplogroup analysis was conducted with logistic regression analysis. The heteroplasmy was analyzed with ANOVA (ANOVA) and Student test. Sequence kernel association tests (SKATs) were performed to analyze the association between mitochondrial genes and KC. Mtoolbox, Mitoclass.1, and APOGEE were used to estimate the impact of the identified variants in protein-coding genes. PON-mt-tRNA was used to annotate the impact of the variants in tRNA. RNAstructure was used to predict the secondary structures of native and mutated tRNAs.

Results: We identified 689 variants in patients with KC and 725 variants in controls (with 308 variants shared by both). The mitochondrial haplogroups exhibited no statistically significant differences between the two groups. Based on the heteroplasmy analysis, the number of heteroplasmic variants in the complete mitochondrial genome, RNA coding regions, and noncoding regions were statistically significantly different in the KC cases and controls (p<0.05). The heteroplasmic levels of the m.16180_16182delAA, m.16182insC, and m.14569 G>C variants in the KC cases were statistically significantly higher than those in the controls (p<0.05). The SKAT analysis showed that the and genes were statistically significantly associated with KC (p<0.05). Among the nine variants of included in the SKAT analysis (m.9300G>A, m.9316T>C, m.9327A>G, m.9355A>G, m.9468A>G, m.9612G>A, m.9804G>A, m.9957G>A, and m.9966 G>A), m.9612G>A was predicted to be deleterious by Mtoolbox. The m.9316T>C, m.9327A>G, m.9355A>G, m.9612G>A, m.9804G>A, and m.9957G>A variants were predicted to be damaging by Mitoclass.1. The m.9355A>G and m.9804G>A variants were predicted to be pathogenic by APOGEE. All identified variants located in (m.12153C>T, m.12178C>T, and m.12192G>A) were predicted to be neutral by the PON-mt-tRNA website.

Conclusions: This study presents the mitochondrial gene profile of Chinese patients with KC and demonstrated that the and genes were associated with KC.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116249PMC
May 2021

Hyaluronidase-responsive phototheranostic nanoagents for fluorescence imaging and photothermal/photodynamic therapy of methicillin-resistant infections.

Biomater Sci 2021 Jun;9(12):4484-4495

State Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Centre for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing 210023, China.

Infectious diseases associated with antibiotic-resistant bacteria are ever-growing threats to public health. Effective treatment and detection methods of bacterial infections are in urgent demand. Herein, novel phototheranostic nanoagents ([email protected] nanosheets, MHC NSs) with hyaluronidase (HAase)-responsive fluorescence imaging (FLI) and photothermal/photodynamic therapy (PTT/PDT) functions were prepared. In this design, Ce6 is used as both a photosensitizer and a fluorescent probe, while MoS2 nanosheets (MoS2 NSs) serve as both a fluorescence quencher and a photothermal agent. Hyaluronic acid conjugated with Ce6 (HA-Ce6) was assembled on the surface of MoS2 NSs to form MHC NSs. Without the HAase secreted by methicillin-resistant Staphylococcus aureus (MRSA), the fluorescence of Ce6 is quenched by MoS2 NSs, while in the presence of MRSA, HAase can degrade the HA and release Ce6, which restores the fluorescence and photodynamic activity of Ce6. The experimental results show that MHC NSs can fluorescently image the MRSA both in vitro and in vivo by HAase activation. Meanwhile, MHC NSs can serve as PTT/PDT dual-mode antibacterial agents for MRSA. In vitro antibacterial results show that MHC NSs can kill 99.97% MRSA under 635 nm and 785 nm laser irradiation. In vivo study further shows that MHC NSs can kill 99.9% of the bacteria in MRSA infected tissues in mice and prompt wound healing by combined PTT/PDT. This work provides novel HAase-responsive phototheranostic nanoagents for effective detection and treatment of bacterial infections.
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http://dx.doi.org/10.1039/d1bm00406aDOI Listing
June 2021

Effects of Different Patterns and Sources of Trace Elements on Laying Performance, Tissue Mineral Deposition, and Fecal Excretion in Laying Hens.

Animals (Basel) 2021 Apr 19;11(4). Epub 2021 Apr 19.

College of Animal Science and Technology, Hunan Agricultural University, No. 1 Nongda Road, Furong District, Changsha 410128, China.

This study was conducted to investigate the effects of different patterns and sources of Zn, Fe, Cu, Mn, and Se on performance, mineral deposition (liver, kidney, pancreas, spleen, pectorals muscle, and tibia), and excretion of laying hens, then to find an optimal dietary supplemental pattern of trace elements in laying hens. A total of 864 healthy laying hens with similar laying rate (Roman, 26-week-old) were randomly divided into nine treatments, with six replications of 16 birds per replication, including a control treatment and four patterns with different element sources (inorganic or organic): (1) Control treatment (basic diet without added extra trace minerals, CT); pattern 1, NRC (1994) recommended level (NRC-L): (2) inorganic minerals of NRC-L pattern (IN), (3) organic minerals of NRC-L pattern (ON); pattern 2, NY/T 33-2004 recommended level (NY/T-L): (4) inorganic minerals of NY/T-L pattern (IY), (5) organic minerals of NY/T-L pattern (OY); pattern 3, 50% NRC (1994) recommended level (50% NRC-L): (6) inorganic minerals of 50% NRC-L pattern (IHN), (7) organic minerals of 50% NRC-L pattern (OHN); pattern 4, the ratio of minerals in blood of laying hens was taken as the supplement proportion of trace elements, and Zn was supplemented depended on NRC recommended level (TLB): (8) inorganic minerals of TLB pattern (IB), (9) organic minerals of TLB pattern (OB). Two weeks were allowed for adjustment to the conditions and then measurements were made over eight weeks. Supplementation of trace elements led to increased daily egg weight ( < 0.05). Patterns of minerals in diets affected the content of liver Mn, pancreas Mn, tibia Mn, and the tissues Se ( < 0.05). Sources of minerals had positive effects on daily egg weight ( < 0.05), the concentrations of liver Fe, kidney Cu, tissues Se (except spleen), and fecal Se ( < 0.05). In conclusion, diet supplemented with the organic trace minerals of 50% NRC-L pattern (OHN) in laying hens promoted optimum laying performance, mineral deposition, and reduced mineral excretion.
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http://dx.doi.org/10.3390/ani11041164DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072985PMC
April 2021

Associations of spicy food flavour and intake frequency with blood lipid levels and risk of abnormal serum lipid levels in Chinese rural population: a cross-sectional study.

Public Health Nutr 2021 Dec 29;24(18):6201-6210. Epub 2021 Apr 29.

Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, HA450001, People's Republic of China.

Objective: The purpose of the current study was to investigate associations between spicy food intake and serum lipids levels in Chinese rural population.

Design: Information on spicy food flavour and intake frequency was obtained using a two-item questionnaire survey. Dietary data were collected using a validated thirteen-item FFQ. Fasting blood samples were collected and measured for total cholesterol (TC), TAG, HDL-cholesterol and LDL-cholesterol. Multivariate linear and logistic regression models were employed to examine the relationship between spicy food and serum lipids levels according to the spicy food flavour and intake frequency, respectively.

Setting: A cross-sectional study in Henan Province.

Participants: 38 238 participants aged 18-79 years old.

Results: Spicy flavour and intake frequency were consistently associated with decreased TC and non-HDL-cholesterol levels but mildly associated with elevated TAG levels. Each level increment in spicy flavour was inversely associated with high TC (OR: 0·91; 95 % CI 0·88, 0·93) and high non-HDL-cholesterol (OR: 0·88; 95 % CI 0·85, 0·91) but positively associated with high TAG (OR: 1·04; 95 % CI 1·01, 1·07). Similarly, 1-d increment in spicy food intake frequency was also inversely associated with high TC (OR: 0·92; 95 % CI 0·91, 0·94) and high non-HDL-cholesterol (OR: 0·91; 95 % CI 0·89, 0·93) but positively associated with high TAG (OR: 1·04; 95 % CI 1·02, 1·06).

Conclusions: Spicy food intake was mildly associated with increased risk of abnormal TAG level, significantly associated with decreased risk of abnormal TC and non-HDL levels. Spicy food intake may be contribute to the management of lipid levels.
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http://dx.doi.org/10.1017/S1368980021001853DOI Listing
December 2021

A hospital-based study on clinical data, demographic data and visual function of keratoconus patients in Central China.

Sci Rep 2021 04 6;11(1):7559. Epub 2021 Apr 6.

Henan Provincial People's Hospital, Henan Eye Hospital, Henan Eye Institute, People's Hospital of Zhengzhou University, Henan University People's Hospital, 7 Weiwu Road, Zhengzhou, 450003, Henan, People's Republic of China.

China is a populous country but lacks epidemiological data on keratoconus (KC). The present study aimed to investigate the clinical data, demographic data, and visual function (VF) data of KC patients in Central China. A total of 524 KC eyes in 307 KC patients (217 bilateral and 90 unilateral) from Henan Eye Hospital were included in the current study. Demographic and VF data were assessed with questionnaires administered by well-trained staff during face-to-face interviews. Visual acuity value was examined by a qualified optometrist, and the clinical data were measured by professional clinicians. The distributions of sex, residence and education level of KC patients were compared by Chi-square tests, and the ratios of people wearing glasses and rigid gas permeable (RGP) lenses were compared by McNemar tests. General linear models/Chi-squared tests were used to compare the clinical and demographic data according to KC severity. Spearman's correlation analysis was used to test the associations between the data and KC severity. The mean age at diagnosis was 20.98 ± 6.06 years, and males had a higher ratio of KC than females (P < 0.001). Patients in rural areas had a higher rate of KC than those in urban areas (P = 0.039), and the proportion of KC patients with a higher education level (above high school) was high (P < 0.001). A total of 68.40% of the patients reported eye rubbing and 3.52% had a positive family history. The percentage of people wearing glasses was higher than that of patients wearing RGP lenses (P < 0.001). The total VF score of KC patients was 69.35 ± 15.25. The thinnest corneal thickness (TCT) and stiffness parameter at the first applanation (SP-A1) values were inversely correlated with KC severity (P < 0.05). The mean, steep, and max keratometry (Km, Ks and Kmax) values, the RGP lens use and keratoplasty were positively correlated with KC severity (all P < 0.05). The total VF score of the eye with better VA decreased as the severity increased (r = - 0.21, P = 0.002). The present study comprehensively describes various associated features of KC patients from a tertiary hospital in Central China, providing a reference for understanding the characteristics of KC patients in China.
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http://dx.doi.org/10.1038/s41598-021-87291-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024332PMC
April 2021

Accuracy of new Corvis ST parameters for detecting subclinical and clinical keratoconus eyes in a Chinese population.

Sci Rep 2021 03 2;11(1):4962. Epub 2021 Mar 2.

Henan Provincial People's Hospital, Henan Eye Hospital, Henan Eye Institute, People's Hospital of Zhengzhou University, Henan University People's Hospital, 7 Weiwu Road, Zhengzhou, 450003, Henan, People's Republic of China.

This study aimed to compare the values of new corneal visualization Scheimpflug technology (Corvis ST) parameters in normal, subclinical keratoconus (SKC) and keratoconus (KC) eyes, and evaluate the diagnostic ability to distinguish SKC and KC eyes from normal eyes. One-hundred normal, 100 SKC and 100 KC eyes were included in the study. Corvis ST parameters containing dynamic corneal response parameters were measured by one ophthalmologist. The receiver operating characteristic curve was used to evaluate the diagnostic ability of new Corvis ST parameters. The new Corvis ST parameters in KC eyes were different from those in the control and SKC eyes after adjusting for IOP and CCT, and stiffness parameter at the first applanation (SP-A1) and Corvis biomechanical index (CBI) were significantly different between the control and SKC eyes (all P < 0.05). The parameter with the highest diagnostic efficiency was SP-A1 (Youden index = 0.40, AUC = 0.753), followed by CBI (Youden index = 0.38, AUC = 0.703), and Integrated Radius (Youden index = 0.33, AUC = 0.668) in diagnosing SKC from control eyes. New Corvis ST parameters in SKC eyes were significantly different from normal control and KC eyes, and could be considered to distinguish SKC and KC eyes from normal eyes.
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http://dx.doi.org/10.1038/s41598-021-84370-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7925657PMC
March 2021

Novel homozygous mutation of plasminogen in ligneous conjunctivitis: a case report and literature review.

Ophthalmic Genet 2021 04 11;42(2):105-109. Epub 2021 Jan 11.

Henan Provincial People's Hospital, Henan Eye Hospital, Henan Eye Institute, People's Hospital of Zhengzhou University, Henan University People's Hospital, Zhengzhou, China.

Ligneous conjunctivitis (LC) is a rare disease characterized by the development of a wood-like pseudomembrane on the tarsal conjunctiva secondary to type I plasminogen deficiency. Here we reported on a Chinese patient with LC in a consanguineous family and performed a literature review of all reported mutations for this disease. A 13-month-old girl diagnosed with LC and her parents were included in this study. Hematoxylin and eosin staining was used to perform histopathology examination. The plasminogen activity was determined by chromogenic assay. Sanger sequencing was performed to screen the mutation site for the disease. In silico analysis was applied to predict the pathogenesis of the identified mutation. In addition, we reviewed the literatures on PLG mutations of LC. Histopathology examination revealed the infiltration of inflammatory cells on membranous lesions. Plasma plasminogen activity was severely decreased in the patient and moderately decreased in her parents (patient: plasminogen activity, 2.50%; father: plasminogen activity, 41.02%; mother: plasminogen activity, 54.07%). Co-segregation analysis indicated that the patient was homozygous for the c.763 G > A (p.Glu255Lys) mutation in plasminogen gene (). Bioinformatics analysis strongly suggested that the mutation was damaging for the disease. The model analysis indicated the mutation might cause abnormal spatial structure and low stability, thus affecting functional activity. A literature review of the LC mutations indicated a strong genetic heterogeneity of the disease. LC exhibited strong genetic heterogeneity, and our study identified a novel homozygous missense mutation of plasminogen (c.763 G > A, p.Glu255Lys) in one Chinese patient with LC.
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http://dx.doi.org/10.1080/13816810.2020.1867753DOI Listing
April 2021

The complete chloroplast genome of and phylogenic analysis of Oleaceae.

Mitochondrial DNA B Resour 2020 18;5(3):2103-2104. Epub 2020 May 18.

School of Life Sciences, Guizhou Normal University, Guiyang, China.

We assembled and characterized the complete chloroplast genome sequence of to investigate its phylogenetic position. The plastome is 156,064 bp in length, which is comprised of a large single-copy (LSC) region of 86,851 bp, a small single-copy (SSC) region of 17,837 bp, and two inverted repeat (IR) regions of 25,688 bp. The overall GC content of the plastome was 37.7. The new sequence comprised total 135 genes, including 87 protein-coding genes, 8 ribosomal RNA genes, and 40 tRNA genes. Phylogenetic analysis showed that was close to
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http://dx.doi.org/10.1080/23802359.2020.1763864DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7510599PMC
May 2020

Endocrine Adverse Events Caused by Different Types and Different Doses of Immune Checkpoint Inhibitors in the Treatment of Solid Tumors: A Meta-Analysis and Systematic Review.

J Clin Pharmacol 2021 03 12;61(3):282-297. Epub 2021 Jan 12.

Department of Endocrinology, the First Hospital of Lanzhou University, Lanzhou, Gansu, China.

The aim of this meta-analysis was to assess the risks of endocrine adverse events in patients with malignancies treated with different types and different doses of immune checkpoint inhibitors (ICIs). PubMed and Embase were searched for randomized controlled trials on ICIs and endocrine adverse events since 2000, and meta-analysis was carried out. Twenty-six randomized controlled trials comprising 13 824 patients with malignancies were included. Compared with the other tumor therapies (used as a control group), patients treated with programmed death-1 inhibitors appeared to be at higher risks of hypothyroidism, hyperthyroidism, thyroiditis, hypophysitis or hypopituitarism, and type 1 diabetes mellitus, while there was no difference in the risk of primary adrenal insufficiency. It was also found that patients treated with cytotoxic T-lymphocyte-associated protein-4 inhibitors were at higher risk of hypophysitis or hypopituitarism, primary adrenal insufficiency, and hypothyroidism. In comparison, patients treated with programmed death-ligand 1 inhibitors were at higher risk of hyperthyroidism and hypothyroidism. Compared with the control group, both low-dose and high-dose ICI groups were at higher risk of hypothyroidism and hyperthyroidism, and the low-dose group had increased risk of thyroiditis and primary adrenal insufficiency. There was no significant difference in the risk of type 1 diabetes between the low-dose group and the high-dose group. The risk of hypophysitis or hypopituitarism in the high-dose group (relative risk, 20.12; 95% confidence interval, 8.02-50.46) was significantly higher than that in the low-dose group (relative risk, 4.92; 95% confidence interval, 2.11-11.47). The risk of endocrine adverse events was increased in patients treated with ICIs. Different types and doses of ICIs have varying characteristics of endocrine adverse events.
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http://dx.doi.org/10.1002/jcph.1804DOI Listing
March 2021

Association between Corneal Stiffness Parameter at the First Applanation and Keratoconus Severity.

J Ophthalmol 2020 2;2020:6667507. Epub 2020 Dec 2.

Henan Provincial People's Hospital, Henan Eye Hospital, Henan Eye Institute, People's Hospital of Zhengzhou University, Henan University People's Hospital, Zhengzhou 450003, China.

Objective: The study aimed to evaluate the character of corneal stiffness parameter at the first applanation (SP-A1) in normal and keratoconus eyes and explore the association between SP-A1 and keratoconus severity indicators.

Methods: A total of 351 normal and 351 keratoconus eyes were included in the current study. Keratoconus was diagnosed according to the corneal topography map and slit-lamp examination. The severity of keratoconus was classified to mild (steep keratometry (Ks) < 48D), moderate (48 ≤ Ks < 55D), and severe (Ks ≥ 55D). The SP-A1 was measured using the Corvis ST software. The correlation analyses and receiver operating characteristic (ROC) curve were performed in the current analysis.

Results: The SP-A1 values of keratoconus were lower than that of normal eyes (72.11 (57.02, 83.08) mmHg/mm vs 110.89 (100.45, 122.47) mmHg/mm, < 0.001). With the severity of keratoconus increasing, the SP-A1 decreased and the value of SP-A1 was 79.54 (70.30, 90.93) mmHg/mm, 65.11 (53.14, 77.46) mmHg/mm, and 47.59 (37.50, 62.14) mmHg/mm in mild, moderate, and severe keratoconus eyes, respectively ( < 0.001). The negative association between SP-A1 and Ks was found in mild, moderate, and severe keratoconus eyes (r  = -0.171,  = -0.317,  = -0.288, all < 0.05). A positive association between SP-A1 and the thinnest corneal thickness (TCT) was found in all eyes (r = 0.687,  = 0.519,  = 0.488,  = 0.382, all < 0.05). SP-A1 was found to be statistically positively associated with intraocular pressure (IOP), biomechanical corrected IOP (bIOP), time from the initiation of air puff until the first applanation (A1T), corneal velocity at the second applanation (A2V), and negatively associated with deformation amplitude (DA), peak distance (PD), corneal velocity at the first applanation (A1V), time from the initiation of air puff until the second applanation (A2T), and DA Ratio Max [2 mm] both in normal and keratoconus eyes (all < 0.05). The ROC analysis indicated that the AUC (95% CI) of SP-A1 was 0.952 (0.934-0.967) and 0.930 (0.904-0.951) in detecting keratoconus eyes and mild keratoconus eyes from normal eyes, respectively.

Conclusions: The SP-A1 value decreased while the keratoconus severity increased. It was lower in keratoconus than that in normal eyes and could be helpful in identifying keratoconus eyes from normal eyes. Further researches would be warranted to expand the clinical utility of SP-A1.
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http://dx.doi.org/10.1155/2020/6667507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7726963PMC
December 2020

Retreatment with immune checkpoint inhibitors in solid tumors: a systematic review.

Ther Adv Med Oncol 2020 27;12:1758835920975353. Epub 2020 Nov 27.

Department of Medical Oncology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Shuai Fu Yuan, Dongcheng District, Beijing 100032, China.

Background: A large proportion of patients eventually experience disease progression despite treatment with immune checkpoint inhibitors (ICIs), but subsequent treatment options are limited for this population. Retreatment with the same or different types of ICIs is a possible strategy, but the clinical efficacy and safety data are limited. This systematic review aims to evaluate the efficacy and safety of ICIs retreatment in patients with solid tumors after disease progression to previous ICIs.

Methods: We searched MEDLINE, EMBASE, the Cochrane Library, and major meeting libraries for prospective studies. The primary outcomes included the objective response rate (ORR), disease control rate (DCR), median overall survival (mOS), and the incidence of grade ⩾3 immune-related adverse events (irAEs).

Results: We identified 22 prospective studies including 1865 patients. For disease progression after CTLA-4 inhibitors, three studies evaluated anti-CTLA-4 retreatment. The ORR was 12-23%, the DCR was 48.4-67.7%, and the mOS was 12 months. The incidence of grade ⩾3 irAEs was 5.9-25%. Four studies evaluated anti-programmed cell death protein 1 (PD-1) retreatment. The ORR was 22-36%, the DCR was 40-64%, and the mOS was 13.4-20.6 months. The incidence of grade ⩾3 irAEs was <10%. For disease progression after PD-(L)1 inhibitors, 13 studies evaluated anti-PD-(L)1 retreatment. The ORR was 5-53%, the DCR was 38-83%, and the mOS was 13.9 months. The incidence of grade ⩾3 irAEs was 0-15% for patients retreated with single anti-PD-(L)1 agent, but was higher (0-64%) for those retreated with ICIs combined with other agents. Two studies evaluated anti-cytotoxic T-lymphocyte associated protein 4 (CTLA-4) retreatment. The ORR was 0-22.4%, the DCR was 50-72%, and the mOS was 4-21 months. The incidence of grade ⩾3 irAEs was 26-61%.

Conclusion: Retreatment with ICIs is feasible for cancer patients considering its encouraging efficacy and tolerable safety. Further prospective trials are needed to explore more promising strategies and identify suitable populations for retreatment.
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http://dx.doi.org/10.1177/1758835920975353DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7705192PMC
November 2020

Effect of the level and source of supplementary dietary zinc on egg production, quality, and zinc content and on serum antioxidant parameters and zinc concentration in laying hens.

Poult Sci 2020 Nov 3;99(11):6233-6238. Epub 2020 Jul 3.

College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; Hunan Co-Innovation Center of Animal Production Safety, Changsha 410128, China. Electronic address:

Zinc is vital for proper functioning of an animal. Two sources of zinc are commonly supplemented in animal feed, organic and inorganic zinc, and there are reports that the former is absorbed to a greater extent than the latter. We hypothesized that supplementary zinc would increase zinc content in eggs of laying hens and that organic zinc would be more effective than inorganic zinc. To test these hypotheses, we examined the effect of levels and sources of supplemental dietary zinc on average daily feed intake (ADFI), egg production, and zinc content in eggs and on serum antioxidant capacity and zinc concentration in laying hens. A total of 720 Roman laying hens (21-week-old) were randomly assigned to 5 treatment groups with 6 replicates, with 24 hens in each replicate. Two sources of zinc, organic (zinc amino acid complex) and inorganic (zinc sulfate), each with 2 levels, low (35 mg/kg) and high (70 mg/kg), comprised 4 treatment groups, and a control group without supplementary zinc was the fifth group. Seven days were allowed for adjustment to the conditions, and then measurements were taken over 42 D. There was no difference in ADFI, average egg weight (EW), ADFI-to-EW ratio, and egg quality (P > 0.05) among the 5 treatment groups; supplemental zinc increased serum concentrations of Zn and Cu-Zn superoxide dismutase and tended to increase superoxide dismutase content (P = 0.065). Zinc content in eggs increased linearly with supplementary organic zinc (N = 18, R = 0.363, P = 0.008) and with supplementary inorganic zinc (N = 18, R = 0.366, P = 0.008) treatment, but there was no difference between the source treatments of zinc. Therefore, our first hypothesis was supported, but our second one was not supported. We concluded that zinc supplementation is effective in enhancing zinc content in eggs and in improving antioxidant capacity in laying hens.
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http://dx.doi.org/10.1016/j.psj.2020.06.029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7647701PMC
November 2020

Mediation effect of body mass index on the association between spicy food intake and hyperuricemia in rural Chinese adults: the Henan rural cohort study.

BMC Public Health 2020 Oct 29;20(1):1629. Epub 2020 Oct 29.

Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, 100 Kexue Avenue, Zhengzhou, 450001, Henan, PR China.

Background: The relationship of spicy food intake with hyperuricemia remains unknown. The objective of this study was to examine the association between spicy food intake and hyperuricemia, and whether this association was mediated by body mass index (BMI) in Chinese rural population.

Methods: 38, 027 adults aged 18-79 years were recruited from the Henan Rural Cohort Study. Information on spicy food intake was obtained using a validated questionnaire survey. Multivariable logistic regression model was used to estimate the association between spicy food intake and hyperuricemia, multiple linear regression model was performed to estimate the relationships between spicy food intake, BMI and serum urate level. BMI was used as a mediator to evaluate the mediation effect.

Results: After adjusting for potential confounders, compared with no spicy food flavor, the odds ratio (OR) and 95% confidence interval (CI) of mild, middle, and heavy flavor for hyperuricemia were 1.09 (1.00-1.19), 1.10 (0.97-1.24), and 1.21 (1.10-1.46), respectively (P = 0.017). Similarly, compared with those without intake in spicy food, the multivariable adjusted OR (95% CI) of 1-2 days/week, 3-5 days/week, and 6-7 days/week were 1.15 (1.01-1.31), 1.14 (1.01-1.30) and 1.15 (1.05-1.26), respectively (P = 0.007). However, when we further controlling for BMI, the associations were substantially attenuated. Furthermore, mediation analysis showed that BMI play a full mediating role in the relationship of spicy food intake with hyperuricemia.

Conclusion: Spicy food flavor and intake frequency are positively related with hyperuricemia in Chinese rural population. BMI may play a full mediating role in the relationship.

Trial Registration: The Henan Rural Cohort Study registered at Chinese Clinical Trial Register (Registration number: ChiCTR-OOC-15006699 ). Date of registration: 2015-07-06.
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http://dx.doi.org/10.1186/s12889-020-09736-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596996PMC
October 2020

Evaluation of the Associations of GC and CYP2R1 Genes and Gene-Obesity Interactions with Type 2 Diabetes Risk in a Chinese Rural Population.

Ann Nutr Metab 2020 24;76(3):175-182. Epub 2020 Sep 24.

Department of Epidemiology and Biostatistics, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China,

Introduction: Group-specific component (GC) and cytochrome P450 family 2 subfamily R member 1 (CYP2R1) gene polymorphisms and obesity have been associated with an increased risk for development of type 2 diabetes mellitus (T2DM) in Asian populations.

Objective: This study assessed the associations of interactions between GC gene variants and CYP2R1 gene variants and between genes and obesity with T2DM risk.

Methods: A study that included 2,271 subjects was performed. Eight single nucleotide polymorphisms in the GC and CYP2R1 genes were genotyped. Interaction analysis was performed using rs7041 in the GC gene and rs1993116 in the CYP2R1 gene. The effects of multiplicative and additive gene-gene and gene-environment interactions on T2DM risk were assessed.

Results: The T2DM risk was significantly associated with being overweight/obese, abdominal obesity, rs7041, and rs1993116. A significant additive interaction between rs1993116 and rs7041 was associated with T2DM. In addition, there was a significant multiplicative interaction between rs7041 and body mass index (BMI) associated with elevated blood glucose levels, and at a higher BMI (>28.47), the G allele carrier showed a stronger effect than the TT genotype.

Conclusions: The interactions between GC rs7041-CYP2R1 rs1993116 and GC rs7041-BMI may explain the mechanisms by which these factors increase the risk of T2DM development.
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http://dx.doi.org/10.1159/000508024DOI Listing
August 2021

Continuous preparation of antimony nanocrystals with near infrared photothermal property by pulsed laser ablation in liquids.

Sci Rep 2020 Sep 15;10(1):15095. Epub 2020 Sep 15.

Key Laboratory for Organic Electronics and Information Displays and Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing, 210023, China.

Antimony nanocrystals (Sb NCs) are of interest in energy storage, catalysis and cancer therapy for its special physical, chemical and biomedical properties. However, methodology challenges still remain in preparation of colloidal Sb NCs, due to the restricted reaction solution systems, high temperature and time costing for common routes. Herein, size controllable colloidal Sb NCs were continuously prepared by pulsed laser ablation of Sb target in different solvents, owning to the metal nanodroplet explosive ejection and thermal evaporation mechanisms. These well dispersed and stable Sb NCs showed excellent photothermal property in the near-infrared-II window.
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http://dx.doi.org/10.1038/s41598-020-72212-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7493941PMC
September 2020

Cu MoS Nanozyme with NIR-II Light Enhanced Catalytic Activity for Efficient Eradication of Multidrug-Resistant Bacteria.

Small 2020 10 6;16(40):e2001099. Epub 2020 Sep 6.

Key Laboratory for Organic Electronics and Information Displays and Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Nanjing, 210023, China.

Nanozymes with unique enzyme-like catalytic properties and versatile functionalities are particularly attractive for the treatment of bacterial infections, especially for combating drug-resistant bacteria. However, inherently low catalytic activity significantly limits their antibacterial performance. Herein, a new near-infrared II (NIR-II) light responsive nanozyme (Cu MoS nanoplates, CMS NPs) is developed for efficient eradication of multidrug-resistant (MDR) bacteria. CMS NPs with intrinsic dual enzyme-like property can generate reactive oxygen species (ROS) by catalysis. Importantly, CMS NPs show NIR-II light enhanced oxidase- and peroxidase-like catalytic activities to improve ROS generation for highly efficient killing of bacteria. In vitro results demonstrate that CMS NPs (40 µg mL ) achieve rapid killing of 8 log MDR Escherichia coli and 6 log MDR Staphylococcus aureus (S. aureus) under NIR-II light irradiation (1064 nm, 1 W cm ) in 10 min. Moreover, CMS NPs exhibit excellent therapeutic efficacy of MDR S. aureus infection in vivo as well as negligible toxicity to cells and animals, indicating their potential use as antibacterial agents. This work provides a novel antibacterial strategy by combining the catalytic generation of ROS and NIR-II photothermal effect of nanozymes for efficient treatment of MDR bacteria-related infections.
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http://dx.doi.org/10.1002/smll.202001099DOI Listing
October 2020

Exome sequencing identification of susceptibility genes in Chinese patients with keratoconus.

Ophthalmic Genet 2020 12 3;41(6):518-525. Epub 2020 Aug 3.

Henan Provincial People's Hospital, Henan Eye Hospital, Henan Eye Institute, People's Hospital of Zhengzhou University, Henan University People's Hospital , Zhengzhou, China.

Purpose: Keratoconus (KC) is a corneal ectasia disease with complex genetic heterogeneity. The present study aimed to identify susceptibility genes in Chinese patients with KC.

Methods: Exome sequencing (ES) was performed in 28 Chinese KC patients to search for susceptibility genes of the disease. The candidate variants were filtered out by multi-step bioinformatics analysis and validated by Sanger sequencing. Another 100 individuals with KC were also recruited to verify those variants by Sanger sequencing.

Results: By filtering out nonsynonymous variants located in exon, selecting variants which were presented in two or more samples and applying public databases to remove common variants, along with the inclusion of missense SNVs located in differential expressed genes and protein damaging variants (stop gain/stop loss SNVs and InDels), we have identified 6 SNVs (4 missense SNVs: c.1168 T > C in , c.341A>T in , c.4346 T > C in , c.1730A>C in ; 2 stop gain SNVs: c.1138 C > T in , c.241 C > T in ) and 2 InDels (c.193_195del in , c.1690_1698del in ) as candidate variants for KC. The verifying results showed that c.341A>T in and c.193_195del in was found in one and two samples, respectively.

Conclusions: Our study suggested that a total of six SNVs in six genes and two InDels in two genes might be considered as candidate variants in Chinese patients with KC.
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http://dx.doi.org/10.1080/13816810.2020.1799415DOI Listing
December 2020

Efficacy of Immune Checkpoint Inhibitors in Non-small-cell Lung Cancer Patients With Different Metastatic Sites: A Systematic Review and Meta-Analysis.

Front Oncol 2020 9;10:1098. Epub 2020 Jul 9.

Department of Medical Oncology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Organ-specific response patterns reported in previous studies indicate different response toward immune checkpoint inhibitors (ICIs) in non-small-cell lung cancer (NSCLC) patients with different metastatic sites. This study aims to compare the efficacy of ICIs with conventional therapy in NSCLC patients with bone, brain or liver metastases. MEDLINE, Embase, and CENTRAL were searched for studies comparing ICIs with conventional therapy in NSCLC patients with bone, brain or liver metastases. The pooled hazard ratio (HR) of overall survival (OS) and progression-free survival (PFS) among included studies was analyzed using the random effects model. Eight studies consisting of 988 NSCLC patients were included, 259 with brain metastases and 729 with liver metastases. No available study with bone metastases information was identified. For patients with brain metastases, ICIs significantly improved their OS (HR, 0.57; = 0.007). For patients with liver metastases, both OS (HR, 0.72; = 0.006), and PFS (HR, 0.72; = 0.004) improvements were observed in the ICI treatment arm. Subgroup analysis was conducted based on target of ICIs and treatment regimen. PD-1 inhibitors could benefit patients with liver or brain metastases on OS and PFS (brain metastases: OS, HR, 0.43; < 0.001; liver metastases: PFS, HR, 0.52; = 0.003; OS, HR, 0.66; = 0.001), while PD-L1 inhibitors could not. Patients with brain metastases could only gain OS improvement from ICIs combined with chemotherapy (HR, 0.41; = 0.001), but for patients with liver metastases, the benefit was detected using ICIs single agent (HR, 0.68; = 0.012) or ICIs combined with chemotherapy plus anti-VEGF therapy (HR, 0.52; = 0.005). ICIs could significantly improve OS in NSCLC patients with brain or liver metastases compared with conventional therapy. Patients with brain metastases could only gain OS benefit from ICIs combined with chemotherapy, while those with liver metastases obtained superior OS from ICIs single agent or ICIs combined with chemotherapy plus anti-VEGF therapy.
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http://dx.doi.org/10.3389/fonc.2020.01098DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7363957PMC
July 2020

Vitamin D Status and Correlation with Glucose and Lipid Metabolism in Gansu Province, China.

Diabetes Metab Syndr Obes 2020 7;13:1555-1563. Epub 2020 May 7.

Department of Endocrinology, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, People's Republic of China.

Objective: To investigate the relationship between serum 25-hydroxyvitamin D [25(OH)D] levels and blood glucose and lipid levels in people over 18 years of age in Gansu, China.

Subjects And Methods: A total of 1928 volunteers (958 males and 970 females) were selected. The prevalence of abnormal glucose metabolism and lipid metabolism in the vitamin D deficiency group (<20 ng/mL) and the non-vitamin D deficiency group (≥20 ng/mL) were compared. The correlations between serum 25(OH)D and blood glucose and lipid were analyzed.

Results: A total of 1681 patients had 25(OH)D deficiency, with an overall prevalence of 87.2% (82.9% in males and 91.4% in females). The levels of 25(OH)D in the diabetic group and the IGT/IFG group were significantly lower than that in the normal group. The level of 25(OH)D was significantly lower in the dyslipidemia group than that in the normal group, and was significantly lower in the fasting plasma glucose (FPG) ≥5.6 mmol/L group than that in the FPG <5.6 mmol/L group (=0.002). The 25(OH)D level in the serum triglyceride (TG) ≥1.7 mmol/L group was significantly lower than that of the TG <1.7 mmol/L group (=0.0274). The age, heart rate, TG, TC, FPG and HPG levels in the vitamin D deficiency group were significantly higher than those in the non-vitamin D deficiency group (<0.05). The prevalence of FPG ≥5.6 mmol/L in the vitamin D deficiency group was higher than that in the non-vitamin D deficiency group (23.5% vs 16.6%, =0.016). Multiple linear regression analysis suggested that serum 25(OH)D levels were independently correlated with gender, age, FPG, TG and heart rate (β=-0.218, -0.129, -0.075, β=-0.103, -0.058, all <0.05).

Conclusion: The incidences of dyslipidemia and dysglycemia were higher in the vitamin D deficiency group. The vitamin D level was independently and negatively correlated with FPG and TC, but not with waist circumference, BMI and blood pressure.
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http://dx.doi.org/10.2147/DMSO.S249049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216298PMC
May 2020

Assessment of Visual Quality in Eyes with Forme Fruste Keratoconus and Mild and Moderate Keratoconus Based on Optical Quality Analysis System II Parameters.

J Ophthalmol 2020 29;2020:7505016. Epub 2020 Feb 29.

Henan Provincial People's Hospital, Henan Eye Hospital, Henan Eye Institute, People's Hospital of Zhengzhou University, Henan University People's Hospital, Zhengzhou 450003, China.

Purpose: The study aimed to evaluate the visual quality of forme fruste keratoconus (FFK) and mild and moderate keratoconus by using an optical quality analysis system II (OQAS-II) and to explore the correlation between optical quality parameters and the disease progression.

Methods: Twenty-one normal eyes, twenty-one FFK eyes, twenty-one mild keratoconus eyes, and twenty-one moderate keratoconus eyes were included in this prospective study. The optical quality parameters, such as object scatter index (OSI), modulation transfer function cutoff (MTF cutoff), strehl ratio (SR), and OQAS-II values at contrasts of 100% (OV-100), 20% (OV-20), and 9% (OV-9), were measured by OQAS-II. The repeatability of these parameters was analyzed by intraclass correlation coefficient (ICC), repeatability coefficient (RC), and coefficient of variation (CV). Correlations between optical quality parameters and mean central keratometry readings ( ) were evaluated. The sensitivity and specificity of the parameters were analyzed using the receiver operating characteristic (ROC).

Results: All the optical quality parameters among four groups showed good repeatability (all ICC≥0.75). The MTF cutoff, SR, OV-100, OV-20, OV-9 in FFK, mild and moderate keratoconus eyes were significantly lower than those in the normal group (all < 0.05). The ROC analyses of the MTF cutoff, SR, OV-100, OV-20, and OV-9 showed significant area under the curve (AUC) in discriminating FFK form normal, mild keratoconus from FFK, and moderate keratoconus from mild keratoconus (all < 0.05). The ROC analyses of the MTF cutoff, SR, OV-100, OV-20, and OV-9 showed significant area under the curve (AUC) in discriminating FFK form normal, mild keratoconus from FFK, and moderate keratoconus from mild keratoconus (all < 0.05). The ROC analyses of the MTF cutoff, SR, OV-100, OV-20, and OV-9 showed significant area under the curve (AUC) in discriminating FFK form normal, mild keratoconus from FFK, and moderate keratoconus from mild keratoconus (all < 0.05). The ROC analyses of the MTF cutoff, SR, OV-100, OV-20, and OV-9 showed significant area under the curve (AUC) in discriminating FFK form normal, mild keratoconus from FFK, and moderate keratoconus from mild keratoconus (all < 0.05). The ROC analyses of the MTF cutoff, SR, OV-100, OV-20, and OV-9 showed significant area under the curve (AUC) in discriminating FFK form normal, mild keratoconus from FFK, and moderate keratoconus from mild keratoconus (all ) were evaluated. The sensitivity and specificity of the parameters were analyzed using the receiver operating characteristic (ROC).  = -0.710, < 0.05). The ROC analyses of the MTF cutoff, SR, OV-100, OV-20, and OV-9 showed significant area under the curve (AUC) in discriminating FFK form normal, mild keratoconus from FFK, and moderate keratoconus from mild keratoconus (all.

Conclusion: The repeatability of OQAS-II is good in measuring visual quality of normal as well as FFK, mild, and moderate keratoconus. The visual quality of the FFK, mild, and moderate keratoconus is worse than that in normal eyes. The OQAS-II has the potential value in screening FFK from normal eyes and might be a useful tool for evaluating the progression of keratoconus.
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http://dx.doi.org/10.1155/2020/7505016DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125474PMC
February 2020

Evaluation of new Corvis ST parameters in normal, Post-LASIK, Post-LASIK keratectasia and keratoconus eyes.

Sci Rep 2020 03 30;10(1):5676. Epub 2020 Mar 30.

Henan Provincial People's Hospital, Henan Eye Hospital, Henan Eye Institute, People's Hospital of Zhengzhou University, Henan University People's Hospital, Zhengzhou, 450003, China.

The aim of this study was to evaluate the distribution of new Corneal Visualisation Scheimpflug Technology (Corvis ST) parameters in normal, Post-laser in situ keratomileusis (LASIK), Post-LASIK keratectasia (KE) and keratoconus (KC) eyes, and explore the diagnostic ability of these parameters in distinguishing KE from LASIK eyes. Twenty-three normal eyes, 23 LASIK eyes, 23 KE eyes and 23 KC eyes were recruited in this study. The following new Corvis ST parameters were measured: Max Inverse Radius, deformation amplitude (DA) Ratio Max [2 mm], Pachy Slope, DA Ratio Max [1 mm], Ambrosio's relational thickness horizontal (ARTh), Integrated Radius, stiffness parameter at first applanation (SP-A1) and Corvis biomechanical index (CBI). The general linear model, linear regression model, relation analysis and receiver operating characteristic (ROC) curve were performed. The Max Inverse Radius, DA Ratio Max [2 mm], Pachy Slope, DA Ratio Max [1 mm], Integrated Radius and CBI in LASIK eyes, KE eyes and KC eyes were higher than in normal eyes, while the ARTh and SP-A1 parameters were lower than in normal eyes. The KE eyes had higher Max Inverse Radius, DA Ratio Max [2 mm], Pachy Slope, DA Ratio Max [1 mm], Integrated Radius, and lower SP-A1 value than LASIK eyes (all P < 0.05). The central corneal thickness was related to the Pachy Slope (r = -0.485), ARTh (r = -0.766), SP-A1 (r = 0.618) in KE eyes (all P < 0.05). The area under the ROC curve of Integrated Radius, Max Inverse Radius, DA Ratio Max [2 mm] and SP-A1 were above 0.800 in identifying KE from LASIK eyes. Thus, the new Corvis ST parameters were different between LASIK and KE eyes, suggesting that they might be helpful in distinguishing KE eyes from LASIK eyes. However, a further multi-center and large sample study is necessary to confirm these findings.
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http://dx.doi.org/10.1038/s41598-020-62825-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7105482PMC
March 2020

Assessment of Corneal Pachymetry Distribution and Morphologic Changes in Subclinical Keratoconus with Normal Biomechanics.

Biomed Res Int 2019 19;2019:1748579. Epub 2019 Nov 19.

Aier School of Ophthalmology, Central South University, Changsha, China.

Purpose: To investigate the pachymetry distribution of central cornea and morphologic changes in subclinical keratoconus with normal biomechanics and determine their potential benefit for the screening of very early keratoconus.

Methods: This retrospective comparative study was performed in 33 clinically unaffected eyes with normal topography and biomechanics from 33 keratoconus patients with very asymmetric ectasia (VAE-NTB; Corvis Biomechanical Index defined) and 70 truly normal eyes from 70 age-matched subjects. Corneal topographic, tomographic, and biomechanical metrics were measured using Pentacam and Corvis ST. The distance and pachymetry difference between the corneal thinnest point and the apex were defined as D and DP, respectively, to evaluate the pachymetry distribution within the central cornea. The discriminatory power of metrics was analysed via the receiver operating characteristic curve. A logistic regression analysis was used to establish predictive models.

Results: The parameters, D and DP, were significantly higher in VAE-NTB than those in normal eyes. For differentiating normal and VAE-NTB eyes, the Belin-Ambrósio deviation (BAD-D) showed the largest area under the curve (AUC; 0.799), followed by ARTmax (0.798), D (0.771), tomography and biomechanical index (0.760), maximum pachymetry progression index (PPImax, 0.756), DP (0.753), and back eccentricity (B_Ecc, 0.707) with no statistically significant differences among these AUCs. In the VAE-NTB group, the parameter B_Ecc was significantly and positively correlated with D (=0.011) and DP (=0.035), whereas the posterior elevation difference had a significant positive association with DP (=0.042). A model using the indices D, B_Ecc, PPImax, and index of height asymmetry demonstrated the highest AUC of 0.846 with 91.43% specificity.

Conclusions: Abnormal pachymetry distribution within the central cornea and subtle morphologic changes are detectable in subclinical keratoconus with normal biomechanics. This may improve VAE-NTB eyes detection.
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http://dx.doi.org/10.1155/2019/1748579DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6885829PMC
April 2020
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