Publications by authors named "Kai Zhang"

3,487 Publications

  • Page 1 of 1

Spatio-temporal transcriptome profiling and subgenome analysis in the Brassica napus.

Plant J 2022 Jun 28. Epub 2022 Jun 28.

Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City and Southwest University, College of Agronomy and Biotechnology, Southwest University, Beibei, Chongqing, 400715, China.

Brassica napus is an important oil crop and an allotetraploid species. However, the detailed analysis of gene function and homoeologous gene expression in all tissues at different developmental stages was not explored. In this study, we performed a global transcriptome analysis of 24 vegetative and reproductive tissues at six developmental stages (totally 111 tissues). These samples were clustered into eight groups. The gene functions of silique pericarp were similar to roots, stems, and leaves. In particular, glucosinolate metabolic process was associated with root and silique pericarp. Genes involved in protein phosphorylation was often associated with stamen, anther, and the early developmental stage of seeds. Transcription factor (TF) genes were more specific than structural genes. A total of 17,100 genes that were preferentially expressed in one tissue (tissue-preferred genes, TPGs), including 889 TFs (5.2%), were identified in the 24 tissues. Some TPGs were identified as hub genes in the co-expression network analysis, and some TPGs in different tissues were involved in different hormone pathways. About 67.0% of the homoeologs showed balanced expression, whereas biased expression of homoeologs was associated with structural divergence. In addition, the spatiotemporal expression of homoeologs were related to the presence of transposable elements (TEs) and regulatory elements (REs); more TEs and fewer REs in the promoters resulted in divergent expression in different tissues. This study provides a valuable transcriptional map for understanding the growth and development of B. napus, for identifying important genes for future crop improvement, and for exploring gene expression patterns in the B. napus.
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http://dx.doi.org/10.1111/tpj.15881DOI Listing
June 2022

Recent Advances in Near-Infrared-II Fluorescence Imaging for Deep-Tissue Molecular Analysis and Cancer Diagnosis.

Small 2022 Jun 28:e2202035. Epub 2022 Jun 28.

Beijing Key Laboratory for Bioengineering and Sensing Technology, Research Centre for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 10083, P. R. China.

Fluorescence imaging with high sensitivity and minimal invasiveness has received tremendous attention, which can accomplish visualized monitoring and evaluation of cancer progression. Compared with the conventional first near-infrared (NIR-I) optical window (650-950 nm), fluorescence imaging in the second NIR optical window (NIR-II, 950-1700 nm) exhibits deeper tissue penetration capability and higher temporal-spatial resolution with lower background interference for achieving deep-tissue in vivo imaging and real-time monitoring of cancer development. Encouraged by the significant preponderances, a variety of multifunctional NIR-II fluorophores have been designed and fabricated for sensitively imaging biomarkers in vivo and visualizing the treatment procedure of cancers. In this review, the differences between NIR-I and NIR-II fluorescence imaging are briefly introduced, especially the advantages of NIR-II fluorescence imaging for the real-time visualization of tumors in vivo and cancer diagnosis. An important focus is to summarize the NIR-II fluorescence imaging for deep-tissue biomarker analysis in vivo and tumor tissue visualization, and a brief introduction of NIR-II fluorescence imaging-guided cancer therapy is also presented. Finally, the significant challenges and reasonable prospects of NIR-II fluorescence imaging for cancer diagnosis in clinical applications are outlined.
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http://dx.doi.org/10.1002/smll.202202035DOI Listing
June 2022

Biodegradation of polyester polyurethane by the marine fungus Cladosporium halotolerans 6UPA1.

J Hazard Mater 2022 Jun 18;437:129406. Epub 2022 Jun 18.

Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, 178 Daxue Road, Xiamen 361005, PR China; School of Marine Sciences, Nanjing University of Information Science & Technology, Nanjing 210044, PR China; Co-Innovation Center of Jiangsu Marine Bioindustry Technology, Jiangsu Ocean University, Lianyungang 222005, PR China. Electronic address:

Lack of degradability and the accumulation of polymeric wastes increase the risk for the health of the environment. Recently, recycling of polymeric waste materials becomes increasingly important as raw materials for polymer synthesis are in short supply due to the rise in price and supply chain disruptions. As an important polymer, polyurethane (PU) is widely used in modern life, therefore, PU biodegradation is desirable to avoid its accumulation in the environment. In this study, we isolated a fungal strain Cladosporium halotolerans from the deep sea which can grow in mineral medium with a polyester PU (Impranil DLN) as a sole carbon source. Further, we demonstrate that it can degrade up to 80% of Impranil PU after 3 days of incubation at 28 ℃ by breaking the carbonyl groups (1732 cm) and C-N-H bonds (1532 cm and 1247 cm) as confirmed by Fourier-transform infrared (FTIR) spectroscopy analysis. Gas chromatography-mass spectrometry (GC-MS) analysis revealed polyols and alkanes as PU degradation intermediates, indicating the hydrolysis of ester and urethane bonds. Esterase and urease activities were detected in 7 days-old cultures with PU as a carbon source. Transcriptome analysis showed a number of extracellular protein genes coding for enzymes such as cutinase, lipase, peroxidase and hydrophobic surface binding proteins A (HsbA) were expressed when cultivated on Impranil PU. The yeast two-hybrid assay revealed that the hydrophobic surface binding protein ChHsbA1 directly interacts with inducible esterases, ChLip1 (lipase) and ChCut1 (cutinase). Further, the KEGG pathway for "fatty acid degradation" was significantly enriched in Impranil PU inducible genes, indicating that the fungus may use the degradation intermediates to generate energy via this pathway. Taken together, our data indicates secretion of both esterase and hydrophobic surface binding proteins by C. halotolerans plays an important role in Impranil PU absorption and subsequent degradation. Our study provides a mechanistic insight into Impranil PU biodegradation by deep sea fungi and provides the basis for future development of biotechnological PU recycling.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129406DOI Listing
June 2022

Dedifferentiated liposarcoma (DDLPS) in the rectum: A case report.

J Int Med Res 2022 Jun;50(6):3000605221102081

Department of Colorectal and Anal Surgery, The Second Hospital of Jilin University, Changchun, Jilin Province, China.

Dedifferentiated liposarcoma (DDLPS) is a rare subtype of liposarcoma with a poor prognosis. This current case report describes a rectal DDLPS in a 68-year-old Chinese male that presented with lower abdominal pain and weight loss. Computed tomography and magnetic resonance imaging were undertaken to evaluate the tumour. The patient underwent radical resection of the rectal tumour, sigmoid colostomy and partial ureterectomy. The tumour was positive for mouse double minute 2 by immunohistochemistry. The patient healed well but refused chemotherapy postoperatively for economic reasons. The tumour recurred and metastasized 4 weeks after the operation. After relevant treatment, the patient's condition deteriorated and he died of shock, metabolic acidosis, hyperlactataemia and acute renal failure. The case report also reviews the literature in terms of the clinical diagnosis, treatment and pathological characteristics of rectal DDLPS with the aim of improving the level of diagnosis and treatment.
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http://dx.doi.org/10.1177/03000605221102081DOI Listing
June 2022

Phased small RNA-mediated systemic signaling in plants.

Sci Adv 2022 Jun 24;8(25):eabm8791. Epub 2022 Jun 24.

Department of Plant Pathology, University of Kentucky, Lexington, KY 40546, USA.

Systemic acquired resistance (SAR) involves the generation of systemically transported signal that arms distal plant parts against secondary infections. We show that two phased 21-nucleotide (nt) RNAs (tasi-RNA) derived from and synthesized within 3 hours of pathogen infection are the early mobile signal in SAR. undergoes alternate polyadenylation, resulting in the generation of 555- and 367-nt transcripts. The 555-nt transcripts likely serves as the sole precursor for tasi-RNAs D7 and D8, which cleave () , , and to induce SAR. Conversely, increased expression of represses SAR. Knockout mutations in or RNA silencing components required for tasi-RNA biogenesis compromise SAR without altering levels of known SAR-inducing chemicals. Both tasi-ARFs and the 367-nt transcripts are mobile and transported via plasmodesmata. Together, we show that tasi-ARFs are the early mobile signal in SAR.
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http://dx.doi.org/10.1126/sciadv.abm8791DOI Listing
June 2022

Determination of quality markers for quality control of Zanthoxylum nitidum using ultra-performance liquid chromatography coupled with near infrared spectroscopy.

PLoS One 2022 24;17(6):e0270315. Epub 2022 Jun 24.

Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.

With the increasing demand for quality control in the traditional Chinese medicine industry, there is a need for the development of quality markers and a quick, non-destructive technique for the discrimination of related species. In our previous study, ultra-performance liquid chromatography (UPLC) was used for the simultaneous determination of five compounds, including three alkaloids (nitidine chloride, chelerythrine, and magnoflorine), one flavonoid (aurantiamarin), and one lignan (sesamin). In this study, the simultaneous quantification of the above-mentioned compounds could be used to discriminate the powders of roots from those of stems. To further test the reliability of the five compounds, seventy-two batches of wild and seventy-five batches of cultivated Zanthoxylum nitidum samples collected from Guangdong, Guangxi, and Fujian provinces in China were analyzed by UPLC and near-infrared spectroscopy (NIRS). In general, the quantitative results of UPLC were consistent with those of NIRS, and cultivated Z. nitidum has similar major bioactive compounds as the wild one, as supported by principal component analysis. Consequently, these five major bioactive compounds are suggested as potential quality markers. In addition, the NIRS method with discriminant analysis successfully differentiated Z. nitidum from three related species (Z. avicennae, Z. scandens and Toddalia asiatica) of the Rutaceae family. In summary, this study provides a method for the rapid identification of Z. nitidum and discrimination of root and stem powders, and suggests five compounds as quality markers for the evaluation of Z. nitidum.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0270315PLOS
June 2022

Three-Dimensional Dendritic Au-Ag Substrate for On-Site SERS Detection of Trace Molecules in Liquid Phase.

Nanomaterials (Basel) 2022 Jun 10;12(12). Epub 2022 Jun 10.

Department of Materials Science and Engineering, University of Texas at Arlington, Arlington, TX 76019, USA.

The development of a facile surface-enhanced Raman scattering (SERS) sensor for the on-site detection of trace molecules in liquid phase is a compelling need. In this paper, a three-dimensional (3D) dendritic Au-Ag nanostructure was constructed by a two-step electro displacement reaction in a capillary tube for the on-site liquid phase detection of trace molecules. The multiplasmon resonance mechanism of the dendritic Au-Ag structure was simulated using the finite-difference time domain (FDTD) method. It was confirmed that the highly branched 3D structure promoted the formation of high-density "hot spots" and interacted with the gold nanoparticles at the dendrite tip, gap, and surface to maximize the spatial electric field, which allowed for high signal intensification to be observed. More importantly, the unique structure of the capillary made it possible to achieve the on-site detection of trace molecules in liquids. Using Rhodamine 6G (R6G) solution as a model molecule, the 3D dendritic Au-Ag substrate exhibited a high detection sensitivity (10 mol/L). Furthermore, the developed sensor was applied to the detection of antibacterial agents, ciprofloxacin (CIP), with clear Raman characteristic peaks observed even at concentrations as low as 10 mol/L. The results demonstrated that the 3D dendritic Au-Ag sensor could successfully realize the rapid on-site SERS detection of trace molecules in liquids, providing a promising platform for ultrasensitive and on-site liquid sample analysis.
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http://dx.doi.org/10.3390/nano12122002DOI Listing
June 2022

Microstructure and Phase Evolution of Ti-Al-C-Nb Composites Prepared by In Situ Selective Laser Forming.

Materials (Basel) 2022 Jun 20;15(12). Epub 2022 Jun 20.

School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.

In the present work, a novel Ti-Al-C-Nb composite was prepared using in situ selective laser forming (ISLF). The formation mechanism of the Ti-Al-C-Nb bulks, which were synthesized using elemental titanium, aluminum, and carbon (graphite) powders via ISLF techniques, was investigated. The results showed that the TiAl and TiC phases were the dominant synthesis products during the chemical reactions, and these occurred during the ISLF process. The size of the fine nanoscale crystal TiC grains could reach 157 nm at an energy level of 60 J/mm. The porous structure of the ISLF specimens was disclosed, and an open porosity of 20-44% was determined via the scanning speed and the laser power. Both the high dynamic viscosity and the reactions of the raw powders led to the generation of a considerable number of pores, whereas the specimen processed using 45 W and 100 mm/s possessed the lowest degree of open porosity.
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http://dx.doi.org/10.3390/ma15124351DOI Listing
June 2022

Prostaglandin 2α Promotes Autophagy and Mitochondrial Energy Production in Fish Hepatocytes.

Cells 2022 06 9;11(12). Epub 2022 Jun 9.

Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China.

Fatty liver, characterized by excessive lipid droplet (LD) accumulation in hepatocytes, is a common physiological condition in humans and aquaculture species. Lipid mobilization is an important strategy for modulating the number and size of cellular LDs. Cyclooxygenase (COX)-mediated arachidonic acid derivatives are known to improve lipid catabolism in fish; however, the specific derivatives remain unknown. In the present study, we showed that serum starvation induced LD degradation via autophagy, lipolysis, and mitochondrial energy production in zebrafish hepatocytes, accompanied by activation of the COX pathway. The cellular concentration of PGF2α, but not other prostaglandins, was significantly increased. Administration of a COX inhibitor or interference with PGF2α synthase abolished serum deprivation-induced LD suppression, LD-lysosome colocalization, and expression of autophagic genes. Additionally, exogenous PGF2α suppressed the accumulation of LDs, promoted the accumulation of lysosomes with LD and the autophagy marker protein LC3A/B, and augmented the expression of autophagic genes. Moreover, PGF2α enhanced mitochondrial accumulation and ATP production, and increased the transcript levels of β-oxidation- and mitochondrial respiratory chain-related genes. Collectively, these findings demonstrate that the COX pathway is implicated in lipid degradation induced by energy deprivation, and that PGF2α is a key molecule triggering autophagy, lipolysis, and mitochondrial development in zebrafish hepatocytes.
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http://dx.doi.org/10.3390/cells11121870DOI Listing
June 2022

Widespread occurrence of emerging E-waste contaminants - Liquid crystal monomers in sediments of the Pearl River Estuary, China.

J Hazard Mater 2022 Jun 15;437:129377. Epub 2022 Jun 15.

Research Centre for the Oceans and Human Health, City University of Hong Kong Shenzhen Research Institute, Shenzhen 518057, China; School of Energy and Environment and State Key Laboratory of Marine Pollution, City University of Hong Kong, Hong Kong, China. Electronic address:

Liquid crystal monomers (LCMs), commonly used in screens of electronic devices, have recently been identified as a group of emerging chemicals of concern associated with e-waste. They are potentially persistent, bioaccumulative, and toxic substances, and may pose a threat to the marine ecosystem. The Pearl River Estuary (PRE) receives organic contaminants discharged from the Pearl River Delta region, where primitive handling of e-waste is widespread. However, information on the pollution status of LCMs in the PRE is absent. Herein, a rapid and robust analytical method was established using ultrasonic extraction, solid phase extraction cleanup, and GC-Orbitrap-MS analysis. The spatial distribution of 39 target LCMs was investigated in 45 surface sediment samples from the PRE. Ten LCMs were detected, with ΣLCMs ranged from 0.9 to 31.1 ng/g dry weight. Our results demonstrated a widespread occurrence of LCMs in the sediments of the PRE, and a gradient of their contamination from inshore to offshore regions, indicating land-based origins. Our reported ΣLCMs concentrations were relatively higher compared to many other legacy and emerging pollutants found in the same investigated area. Preliminary risk assessment showed 3VbcH, Pe3bcH and tFMeO-3bcHP might be the top 3 risk contributors in the PRE. Further investigation on the ecological impact of LCMs on marine benthic ecosystems, as well as identification of their sources and control measures are warranted.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129377DOI Listing
June 2022

Engineering of Stable Cross-Linked Multilayers Based on Thermo-Responsive PNIPAM--Chitosan/Heparin to Tailor Their Physiochemical Properties and Biocompatibility.

ACS Appl Mater Interfaces 2022 Jun 23. Epub 2022 Jun 23.

Department of Biomedical Materials, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, 06120 Halle (Saale), Germany.

The thermo-responsive poly(-isopropylacrylamide) (PNIPAM) is ubiquitously applied in controlled drug release and tissue engineering. However, the lack of bioactivity of PNIPAM restricts its use in cell-containing systems being a thermo-responsive adhesive substratum with no regulating effect on cell growth and differentiation. In this study, integrating PNIPAM with chitosan into PNIPAM--chitosan (PNIPAM-Chi) allows a layer-by-layer assembly with bioactive heparin to fabricate PNIPAM-modified polyelectrolyte multilayers (PNIPAM-PEMs). Grafting PNIPAM chains of either 2 (LMW) or 10 kDa (HMW) on the chitosan backbone influences the cloud point (CP) temperature in the range from 31 to 33 °C. PNIPAM-Chi with either a higher molecular weight or a higher degree of substitution of PNIPAM chains exhibiting a significant increase in diameter above CP as ensured by dynamic light scattering is selected to fabricate PEM with heparin as a polyanion at pH 4. Little difference of layer growth is detected between the chosen PNIPAM-Chi used as polycations by surface plasmon resonance, while multilayers formed with HMW-0.02 are more hydrated and show striking swelling-and-shrinking abilities when studied with quartz crystal microbalance with dissipation monitoring. Subsequently, the multilayers are covalently cross-linked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/-hydroxysuccinimide to strengthen the stability of the systems under physiological conditions. Ellipsometry results confirm the layer integrity after exposure to the physiological buffer at pH 7.4 compared to those without cross-linking. Moreover, significantly higher adhesion and more spreading of C3H10T1/2 multipotent embryonic mouse fibroblasts on cross-linked PEMs, particularly with heparin terminal layers, are observed owing to the bioactivity of heparin. The slightly more hydrophobic surfaces of cross-linked PNIPAM-PEMs at 37 °C also increase cell attachment and growth. Thus, layer-by-layer constructed PNIPAM-PEM with cross-linking represents an interesting cell culture system that can be potentially employed for thermally uploading and controlled release of growth factors that further promotes tissue regeneration.
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http://dx.doi.org/10.1021/acsami.2c05297DOI Listing
June 2022

Fractionation analysis and risk assessment of potential toxic elements in reservoir sediments in central China.

Environ Sci Pollut Res Int 2022 Jun 23. Epub 2022 Jun 23.

School of Geographic Sciences, Xinyang Normal University, Xinyang, 464000, China.

The pollution of potential toxic elements (PTEs) in the environment is a persistent issue and draws public attention constantly. However, there is no study comprehensively assessing the PTE pollution of water supply reservoirs in central China. This study determined the total contents and fractions of seven PTEs in 10 water supply reservoirs of central China, and evaluated PTE pollution risks by adopting various models. The results indicated that PTE pollution in water supply reservoirs was lower comparing that in estuary or river sediments, and Mn (manganese) was the predominant PTE in central China reservoirs. Fractionation analysis revealed that for B1 fraction (exchangeable/acid-soluble fraction) of Mn, Zn (zinc) and B2 fraction (reducible fraction) of Pb (lead) was high, while the effective state portion of Cr (chromium) was small. The monomial potential ecological risk indices and Igeo values were low among most samples, indicating overall modest PTE pollution. However, the results of the individual contamination factor (ICF) suggested that Pb might lead to potential health risks to human beings.
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http://dx.doi.org/10.1007/s11356-022-21464-8DOI Listing
June 2022

Multipopulation-Based Differential Evolution for Large-Scale Many-Objective Optimization.

IEEE Trans Cybern 2022 Jun 22;PP. Epub 2022 Jun 22.

In recent years, numerous efficient many-objective optimization evolutionary algorithms have been proposed to find well-converged and well-distributed nondominated optimal solutions. However, their scalability performance may deteriorate drastically to solve large-scale many-objective optimization problems (LSMaOPs). Encountering high-dimensional solution space with more than 100 decision variables, some of them may lose diversity and trap into local optima, while others may achieve poor convergence performance. This article proposes a multipopulation-based differential evolution algorithm, called LSMaODE, which can solve LSMaOPs efficiently and effectively. In order to exploit and explore the exponential decision space, the proposed algorithm divides the population into two groups of subpopulations, which are optimized with different strategies. First, the randomized coordinate descent technique is applied to 10% of individuals to exploit the decision variables independently. This subpopulation maintains diversity in the decision space to avoid premature convergence into local optimum. Second, the remaining 90% of individuals are optimized with the nondominated guided random interpolation strategy, which interpolates individual among three nondominated solutions randomly. The strategy can guide the population convergent toward the nondominated solutions quickly, meanwhile, maintain good distribution in objective space. Finally, the proposed LSMaODE is evaluated on the LSMOP test suites from the scalability in both decision and objective dimensions. The performance is compared against five state-of-the-art large-scale many-objective evolutionary algorithms. The experimental results show that LSMaODE provides highly competitive performance.
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http://dx.doi.org/10.1109/TCYB.2022.3178929DOI Listing
June 2022

Inferences of SPECT renal dynamic imaging injection quality based on lung and abdominal aorta imaging features.

Ann Nucl Med 2022 Jun 22. Epub 2022 Jun 22.

Department of Nuclear Medicine, Lanzhou University Second Hospital, Cuiyingmen No. 82, Chengguan DistrictGansu Province, Lanzhou, 730030, People's Republic of China.

Objective: To analyze the characteristics of blood flow perfusion images at different injection levels to establish an evaluation standard for renal dynamic imaging injection quality and reduce misdiagnosis.

Methods: Data from 140 single-photon emission computed tomography renal dynamic imaging, collected in our hospital, were retrospectively analyzed. The scans were divided into four groups according to the injection quality: total leakage of the imaging agent (group A), partial leakage (group B), poor bolus injection quality (group C), and good bolus injection quality (group D). The time of appearance and regression of the pulmonary blood perfusion phase, the peak time in the abdominal aorta, and the ratio between peak count and actual drug injection count were analyzed. The renal dynamic imaging was repeated in low-quality examinations, and the comparison between the two exams provided the misdiagnosis rate caused by inadequate injections.

Results: The images of the lungs and abdominal aorta in group A were blurred and indistinguishable; thus, these exams were unreliable. Both appearance and fading time of the bilateral lung shadows were significantly different between groups B, C, and D (p = 0.002 and p = 0.003, respectively). The peak time and peak counting ratio in the abdominal aorta were also significantly different between these groups (p = 0.002 and p < 0.001, respectively). The misdiagnosis rates of renal dynamic imaging in groups A, B, and C due to the different injection levels were significantly different at 94.29%, 77.14%, and 18.29%, respectively.

Conclusions: The times of appearance and regression of the lung shadows and the peak time and peak count ratio in the abdominal aorta in the dynamic renal imaging perfusion phase can help assess the imaging agent injection quality and identify the need for a repeated examination. Improving the imaging agent injection quality can effectively reduce the renal function misdiagnosis rate.
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http://dx.doi.org/10.1007/s12149-022-01750-8DOI Listing
June 2022

The landscape of isoform switches in sepsis: a multicenter cohort study.

Sci Rep 2022 Jun 17;12(1):10276. Epub 2022 Jun 17.

Department of Emergency Medicine, Key Laboratory of Precision Medicine in Diagnosis and Monitoring Research of Zhejiang Province, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, No 3, East Qingchun Road, Hangzhou, 310016, Zhejiang, China.

Sepsis is caused by an uncontrolled inflammatory response, whose underlying mechanisms are not fully understood. It is well known that the majority of human genes can be expressed as alternative isoforms. While isoform switching is implicated in many diseases and is particularly prominent in cancer, it has never been reported in the context of sepsis. Patients presented to the emergency department of three tertiary care hospitals from January 2020 to December 2020 were enrolled. Clinical variables and genome-wide transcriptome of peripheral blood mononuclear cells (PBMC) were obtained. Isoform switching analysis were performed to identify significant isoform switches and relevant biological consequences. A total of 48 subjects with sepsis, involving 42 survivors and 6 non-survivors, admitted to the emergency department of three tertiary care hospitals were enrolled in this study. PBMCs were extracted for RNA sequencing (RNA-seq). Patients (n = 4) with mild stroke or acute coronary syndrome without infection were enrolled in this study as controls. The most frequent functional changes resulting from isoform switching were changes affecting the open reading frame, protein domains and intron retention. Many genes without differences in gene expression showed significant isoform switching. Many genes with significant isoform switches ([Formula: see text]> 0.1) were associated with higher mortality risk, including PIGS, CASP3, LITAF, HBB and RUVBL2. The study for the first time described the landscape of isoform switching in sepsis, including differentially expressed isoform fractions between patients with and without sepsis and survivors and nonsurvivors. The biological consequences of isoform switching, including protein domain loss, signal peptide gain, and intron retention, were identified.
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http://dx.doi.org/10.1038/s41598-022-14231-9DOI Listing
June 2022

[Application of personalized guide plate combined with real-time navigation in repairing mandibular defect using fibula muscle flap].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2022 Jun;36(6):691-697

Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital of Bengbu Medical College, Bengbu Anhui, 233004, P. R. China.

Objective: To explore the application of personalized guide plate combined with intraoperative real-time navigation in repairing of mandibular defect using fibula muscle flap, providing the basis for the precise repair and reconstruction of mandible.

Methods: The clinical data of 12 patients (9 males and 3 females) aged from 23 to 71 years (mean, 55.5 years) between July 2019 and December 2021 were recorded. These patients were diagnosed as benign or malignant mandibular tumors, including 2 cases of ameloblastoma, 6 cases of squamous cell carcinoma, 2 cases of osteosarcoma, 1 case of adenoid cystic carcinoma, and 1 case of squamous carcinoma. All patients were treated with mandibular amputation, and then repaired by double-stacked three-segment fibula muscle flap. Preoperative virtual design scheme and guide plate were performed. During the operation, personalized guide plate combined with real-time navigation was used for fibular osteotomy and shaping. Thin-slice CT examination was performed at 2-3 weeks after operation, and was fitted with the preoperative virtual design scheme. The difference between the distance of bilateral mandibular angles relative to the reference plane in three-dimensional directions (left-right, vertical, and anterior-posterior) and the difference of the medial angle of the lower edge of the mandible reconstructed by fibula were measured, and the mean error of chromatographic fitting degree was calculated.

Results: The guide plate and navigation were applied well, and the fibula shaping and positioning were accurate. The fibula muscle flap survived, the incision healed well, and the occlusal relationship was good. All 12 patients were followed up 1-29 months, with an average of 17 months. There was no significant difference on the distance of bilateral mandibular angles relative to the reference plane in the left-right [(-0.24±1.35) mm; =-0.618, =0.549], vertical [-0.85 (-1.35, 1.40) mm; =-0.079, =0.937], and anterior-posterior [(-0.46±0.78) mm; =-2.036, =0.067] directions. The difference of the medial angle of the lower edge of the mandible reconstructed by fibula was also not significant [(-1.35±4.34)°; =-1.081, =0.303)]. Postoperative CT and preoperative virtual design fitting verified that there was no significant difference in the change of the mandibular angle on both sides, and the average error was (0.47±1.39) mm.

Conclusion: The personalized guide combined with intraoperative real-time navigation improves the accuracy of peroneal muscle flap reconstruction of the mandible, reduces the complications, and provides a preliminary basis for the application of visual intraoperative navigation in fibula muscle flap reconstruction of the mandible.
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http://dx.doi.org/10.7507/1002-1892.202202090DOI Listing
June 2022

comparison of electrocautery-enhanced delivery of lumen-apposing metal stents matching electrosurgical workstations during EUS-guided gallbladder drainage.

Endosc Ultrasound 2022 Jun 8. Epub 2022 Jun 8.

Department of Gastroenterology, Endoscopic Center, Shengjing Hospital of China Medical University, Shenyang, Liaoning Province, China.

Background And Objectives: EUS-guided gallbladder drainage (EUS-GBD) has become one of the recommended treatments for patients with high-risk acute cholecystitis. However, the gallbladder reportedly collapsed due to bile leakage, which was a disadvantage that affects the surgical success rate. Different electrocautery-enhanced delivery of the lumen-apposing metal stents (ECE-LAMSs) using suitable power levels in electrosurgical workstations can increase the surgical success rate and reduce trauma. Therefore, we proposed the use of the ECE-LAMSs and electrosurgical workstations for the first time through ex vivo experiments to adjust the different power levels and select the most suitable electrosurgical power for each ECE-LAMS type.

Methods: We compared three types of ECE-LAMS (9Fr, 10.5Fr, and 10.8Fr) with three types of electrosurgical workstations during EUS-GBD. GBD was simulated ex vivo under the guidance of an ultrasound endoscope. We performed various power tests to elucidate the ideal electric power for different ECE-LAMS combined with the different types of electrosurgical workstations.

Results: For the 10.8Fr ECE-LAMS matched with the Martin, Erbe, and Olympus electrosurgical workstations, the ideal power levels were 200 W, 200 W, and 250W. For the 10.5Fr and 9Fr ECE-LAMS matched with the Martin, Erbe, and Olympus electrosurgical workstations, the ideal power levels were 150 W, 200 W, and 200 W.

Conclusion: During the operations, due to low-power levels in the electrosurgical workstations increased the number of cuts, the EUS images were obviously unclear, which affected the success rates of the operations. We suggested different power levels for the different types of LAMS and their matching electrosurgical workstations, which can increase the surgical success rates and reduce surgical injuries.
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http://dx.doi.org/10.4103/EUS-D-21-00240DOI Listing
June 2022

Two stages of speech envelope tracking in human auditory cortex modulated by speech intelligibility.

Cereb Cortex 2022 Jun 13. Epub 2022 Jun 13.

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, No. 119 South Fourth Ring West Road, Fengtai District, Beijing 100070, China.

The envelope is essential for speech perception. Recent studies have shown that cortical activity can track the acoustic envelope. However, whether the tracking strength reflects the extent of speech intelligibility processing remains controversial. Here, using stereo-electroencephalogram technology, we directly recorded the activity in human auditory cortex while subjects listened to either natural or noise-vocoded speech. These 2 stimuli have approximately identical envelopes, but the noise-vocoded speech does not have speech intelligibility. According to the tracking lags, we revealed 2 stages of envelope tracking: an early high-γ (60-140 Hz) power stage that preferred the noise-vocoded speech and a late θ (4-8 Hz) phase stage that preferred the natural speech. Furthermore, the decoding performance of high-γ power was better in primary auditory cortex than in nonprimary auditory cortex, consistent with its short tracking delay, while θ phase showed better decoding performance in right auditory cortex. In addition, high-γ responses with sustained temporal profiles in nonprimary auditory cortex were dominant in both envelope tracking and decoding. In sum, we suggested a functional dissociation between high-γ power and θ phase: the former reflects fast and automatic processing of brief acoustic features, while the latter correlates to slow build-up processing facilitated by speech intelligibility.
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http://dx.doi.org/10.1093/cercor/bhac203DOI Listing
June 2022

Water-Phase Lateral Interconnecting Quantum Dots as Free-Floating 2D Film Assembled by Hydrogen-Bonding Interactions to Acquire Excellent Electrocatalytic Activity.

ACS Nano 2022 Jun 13. Epub 2022 Jun 13.

School of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, China.

Supramolecular self-assembly of nanoparticles in two orthogonal directions would potentially allow one to fabricate nanomaterials with fascinating properties. In this study of a hydrothermal polycondensation of melamine/cyanuric acid, graphitic carbon nitride-based quantum dots (CNQD, ∼2 nm) are in situ arranged along two orthogonal directions through lateral hydrogen bonding, and free-floating two-dimensional hydrogen-bonded films of CNQD (2D CNQD) are built. On the basis of the universality of this hydrothermal in situ supramolecular self-assembly technique, 2D films linked by other quantum dots such as sulfur-doped graphitic carbon nitride and CdTe are also constructed. With the benefits of stimuli responsiveness and the reversibility of hydrogen bonds, controllable assembly/disassembly of the 2D CNQD film is feasibly achieved by external stimuli such as inletting CO/N, which endows the assembled 2D CNQD films optimal electrochemical superiorities of both 2D film and zero-dimensional (0D) quantum dots. Accordingly, the 2D CNQD film delivers a high bifunctional activity in both a nitrogen reduction reaction (NRR) and an oxygen evolution reaction (OER). Especially in NRR, it exhibits the high yield rate of NH reaching 75.07 μg h mg at -0.85 V versus reversible hydrogen electrode at ambient condition. Strikingly, the power density of the rechargeable Zn-N battery using 2D CNQD film as cathode reaches 31.94 mW cm, outperforming the majority of Zn-N batteries. Density functional theory calculations proved the promoted adsorption of N and stabilized NRR intermediates on 2D CNQD cooperated by multiply hydrogen-bonding interactions are the main reasons for the excellent NRR electrocatalytic performances. This work hints that hydrothermal in situ supramolecular self-assembly is a feasible and direct way to integrate 0D quantum dots into 2D directional arrays, and the hydrogen bond that interlinks enables this free-floating 2D structure to maintain the electrochemical superiority of both 0D and 2D structures.
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http://dx.doi.org/10.1021/acsnano.2c00507DOI Listing
June 2022

Effects of Underground Coal Mining on Soil Spatial Water Content Distribution and Plant Growth Type in Northwest China.

ACS Omega 2022 Jun 23;7(22):18688-18698. Epub 2022 May 23.

School of Chemical &Environmental Engineering, China University of Mining and Technology, Beijing 100083, China.

The impact of coal mining subsidence on surface ecology involves the influence of several ecological elements such as water, soil, and vegetation, which is systematic and complex. Given the unclear understanding of the synergistic change patterns of the water-soil-vegetation ecological elements in the influence of coal mining in the west, this paper investigates the impact of coal mining on the surface ecology, especially the distribution of soil water content (SWC). In 2020, this study collected 3000 soil samples from 60 sampling points (at depth of 0-10 m) and tested the SWC. All samples come from three different temporal and spatial areas of coal mining subsidence in the desert mining area of Northwest China where soil types are mainly aridisols. At the same time, the interactions among deep SWC and surface soil physical and chemical properties, surface SWC and soil fertility, and pH were analyzed. The spatial variability of soil moisture is reflected by kriging interpolation, and SWC values at different depths are predicted as a basis for monitoring the environmental impact of different coal mining subsidence years. The research has shown that the ground subsidence leads to a decrease in SWC value and changes in surface soil pH, physical and chemical properties, and covering vegetation, which have occurred from the beginning of coal mining. The impact of coal mining on the SWC of the unsaturated zone is mainly at the depth of 0-6 m, where SWC is not directly related to the nutrient content of the surface soil. The overall settlement of the ground will stir up simultaneous decline in the quality of deep SWC and topsoil. The findings of this investigation suggest that changes in the soil structure caused by coal mining subsidence are the key factor in SWC loss. Timely monitoring and repairing 0-6 m ground fissures, as well as selecting shrubs on the surface is the best choice for the restoration of the ecological environment and prevention of soil erosion in this area.
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http://dx.doi.org/10.1021/acsomega.2c01369DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9178752PMC
June 2022

Desmoplastic fibroma of the left 6th rib in a 35-year-old man: A case report.

Asian J Surg 2022 Jun 9. Epub 2022 Jun 9.

Department of Thoracic Surgery, the Third Affiliated Hospital of Sun Yat-sen University, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2022.05.113DOI Listing
June 2022

Highly Efficient Sky-Blue π-Stacked Thermally Activated Delayed Fluorescence Emitter with Multi-Stimulus Response Properties.

Angew Chem Int Ed Engl 2022 Jun 10. Epub 2022 Jun 10.

Soochow University, Institute of Functional Nano & Soft Materials, 199 Ren-ai Road, 215123, Suzhou, CHINA.

Organic materials with multi-stimulus response (MSR) properties have demonstrated many potential and practical applications. Herein, a π-stacked thermally activated delayed fluorescence (TADF) material with multi-stimulus response (MSR) properties, named SDMAC, was designed and synthesized using distorted 9,9-dimethyl-10-phenyl-9,10-dihydroacridine as a donor. SDMAC possesses a rigid π-stacked configuration with intramolecular through space interactions and exhibits aggregation-induced emission enhancement (AIEE), solvatochromic, piezochromic, and circularly polarized luminescence (CPL) under different external stimuli. The rigid molecular structure and efficient TADF properties of SDMAC can use in display and lighting. Using SDMAC as an emitter, the maximum external quantum efficiency (EQE) of the fabricated organic light-emitting diodes (OLEDs) is as high as 28.4%, which is the most efficient CP-TADF OLEDs based on the through-space charge transfer strategy. The CP organic light-emitting diodes (CP-OLEDs) exhibit circularly polarized electroluminescence (CPEL) signals.
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http://dx.doi.org/10.1002/anie.202206861DOI Listing
June 2022

Unilateral versus bilateral pedicle screw fixation with transforaminal lumbar interbody fusion for treatment of lumbar foraminal stenosis.

Spine J 2022 Jun 7. Epub 2022 Jun 7.

Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. Electronic address:

Background Context: Transforaminal lumbar interbody fusion (TLIF) with bilateral pedicle screw fixation (BPSF) is an effective treatment for lumbar foraminal stenosis (LFS). However, the effects of TLIF with unilateral pedicle screw fixation (UPSF) on LFS treatment have not been clearly elucidated.

Purpose: We conducted this study to compare clinical outcomes and radiographic results of TLIF with UPSF and BPSF 2 years after the surgical treatment.

Design: Prospective randomized study.

Patient Sample: This study included 23 patients undergoing TLIF with UPSF and 25 patients undergoing TLIF with BPSF.

Outcome Measures: Clinical outcomes were evaluated by visual analog scale (VAS) for low back pain and leg pain and Oswestry Disability Index (ODI) score. Radiographic outcomes included foraminal height, disc space height, segmental lordosis and final fusion rates.

Methods: The clinical and radiographic outcomes were compared between the UPSF and BPSF group. The postoperative improvements were evaluated in either group. Intraoperative data such as duration of operation and estimated blood loss were collected. This study was registered at clinicaltrials.gov.

Results: Analysis of the VAS and ODI scores showed significant improvements in clinical outcomes within each group. No significant differences between the two groups were noted in the improvements of the VAS and ODI scores. The mean operative duration and blood loss were significantly greater in the BPSF group than in the UPSF group. There were significant improvements in the height of the foramen and intervertebral space and segmental lordosis in both groups, while there was no significant difference between the groups in amount of the improvements. No significant difference was found in the final fusion rates.

Conclusions: TLIF is an appropriate procedure for LFS treatment. With balanced intervertebral support using a cage, UPSF could achieve similar and satisfactory effects on lumbar segmental stability and fusion compared to BPSF. The unilateral approach appears to be associated with slightly shorter operative time and less blood loss.
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http://dx.doi.org/10.1016/j.spinee.2022.05.011DOI Listing
June 2022

Doppler Modeling and Simulation of Train-to-Train Communication in Metro Tunnel Environment.

Sensors (Basel) 2022 Jun 4;22(11). Epub 2022 Jun 4.

Key Laboratory of Specialty Fiber Optics and Optical Access Networks, Joint International Research Laboratory of Specialty Fiber Optics and Advanced Communication, Shanghai Institute for Advanced Communication and Data Science, Shanghai University, Shanghai 200444, China.

The communication system of urban rail transit is gradually changing from train-to-ground (T2G) to train-to-train (T2T) communication. The subway can travel at speeds of up to 200 km/h in the tunnel environment, and communication between trains can be conducted via millimeter waves with minimum latency. A precise channel model is required to test the reliability of T2T communication over a non-line-of-sight (NLoS) Doppler channel in a tunnel scenario. In this paper, the description of the ray angle for a T2T communication terminal is established, and the mapping relationship of the multipath signals from the transmitter to the receiver is established. The channel parameters including the angle, amplitude, and mapping matrix from the transmitter to the receiver are obtained by the ray-tracing method. In addition, the channel model for the T2T communication system with multipath propagations is constructed. The Doppler spread simulation results in this paper are consistent with the RT simulation results. A channel physics modelling approach using an IQ vector phase shifter to achieve Doppler spread in the RF domain is proposed when paired with the Doppler spread model.
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http://dx.doi.org/10.3390/s22114289DOI Listing
June 2022

Effects of Paradigm Color and Screen Brightness on Visual Fatigue in Light Environment of Night Based on Eye Tracker and EEG Acquisition Equipment.

Sensors (Basel) 2022 May 27;22(11). Epub 2022 May 27.

School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

Nowadays, more people tend to go to bed late and spend their sleep time with various electronic devices. At the same time, the BCI (brain-computer interface) rehabilitation equipment uses a visual display, thus it is necessary to evaluate the problem of visual fatigue to avoid the impact on the training effect. Therefore, it is very important to understand the impact of using electronic devices in a dark environment at night on human visual fatigue. This paper uses Matlab to write different color paradigm stimulations, uses a 4K display with an adjustable screen brightness to jointly design the experiment, uses eye tracker and g.tec Electroencephalogram (EEG) equipment to collect the signal, and then carries out data processing and analysis, finally obtaining the influence of the combination of different colors and different screen brightness on human visual fatigue in a dark environment. In this study, subjects were asked to evaluate their subjective (Likert scale) perception, and objective signals (pupil diameter, θ + α frequency band data) were collected in a dark environment (<3 lx). The Likert scale showed that a low screen brightness in the dark environment could reduce the visual fatigue of the subjects, and participants preferred blue to red. The pupil data revealed that visual perception sensitivity was more vulnerable to stimulation at a medium and high screen brightness, which is easier to deepen visual fatigue. EEG frequency band data concluded that there was no significant difference between paradigm colors and screen brightness on visual fatigue. On this basis, this paper puts forward a new index-the visual anti-fatigue index, which provides a valuable reference for the optimization of the indoor living environment, the improvement of satisfaction with the use of electronic equipment and BCI rehabilitation equipment, and the protection of human eyes.
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http://dx.doi.org/10.3390/s22114082DOI Listing
May 2022

One-Step Method for Material Quantitation Using In-Line Tomography With Single Scanning.

IEEE Trans Biomed Eng 2022 Jun 9;PP. Epub 2022 Jun 9.

Objective: Quantitative technique based on In-line phase-contrast computed tomography with single scanning attracts more attention in application due to the flexibility of the implementation. However, the quantitative results usually suffer from artifacts and noise since the phase retrieval and reconstruction are independent ("two-step") without feedback from the original data. The work aims to investigate a method for material quantitation to improve the image quality of In-line tomography within single scanning.

Method: An iterative method based Fresnel diffraction imaging model is developed in this work, which directly reconstructs the refractive index decrement δ and imaginary β of the object from observed data ("one-step"). Moreover, high-quality material decomposition results are obtained by using a linear approximation in the iterative process.

Results: Compared with the existing methods, Our method shows a higher peak signal-to-noise ratio and structural similarity in numerical experimental results. Additionally, the quantitation accuracy of the proposed method is greater than 97.2 % by calculating the equivalent atomic number of the decomposed basic material in the real experiment.

Conclusion: We demonstrate that this one-step method greatly reduces noise and improves quantitative reconstruction and decomposition results.

Significance: This algorithm has the potential for quantitative imaging research using In-line tomography in future biomedical applications.
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http://dx.doi.org/10.1109/TBME.2022.3181153DOI Listing
June 2022

A Heat-Liquefiable Solid Precursor for Ambient Growth of Perovskites with High Tunability, Performance and Stability.

Small Methods 2022 Jun 8:e2200384. Epub 2022 Jun 8.

Guangdong Provincial Key Lab of Nano-Micro Materials Research, School of Chemical Biology and Biotechnology, Shenzhen Graduate School, Peking University, Shenzhen, 518055, P. R. China.

Halide perovskites are intensively studied for applications in optoelectronic devices because of their outstanding properties and relatively low cost. However, the common precursor solutions for perovskite fabrication are rather unstable in the presence of moisture and oxygen, limiting the large-scale low-cost production of perovskite. Herein, water is used counterintuitively to formulate an ambient stable perovskite precursor, which is peculiar in that it is solid at room temperature but becomes a liquid at 75 °C. The non-fluidity of the precursor stemmed from the water-assisted intermediate fiber assembly, conferring high damp air stability. Yet the heat-liquefiability made the precursor highly processible for perovskite growth, and when guided by polyvinyl pyrrolidone coordination with Pb , the perovskite can preferentially grow along the [200] direction, significantly improving the film quality. To demonstrate the utility of the precursor, it has been used to fabricate self-driven halide perovskite photodetectors, which exhibited a low noise current of 2.0 × 10  A Hz , a high specific detectivity up to 1.4 × 10 Jones, and high stability of 20 days of operation with only < 5% external quantum efficiency decay. This type of solid-liquid convertible precursor opens up new opportunities for wider applications of perovskites.
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http://dx.doi.org/10.1002/smtd.202200384DOI Listing
June 2022

Atomic Force Microscopy Tracking of Nanoparticle Migration in Semicrystalline Polymers.

ACS Macro Lett 2022 06 8;11(6):818-824. Epub 2022 Jun 8.

Department of Mechanical Engineering, College of Engineering and Mathematical Sciences, University of Vermont, Burlington, Vermont 05405, United States.

We present tracking of silica nanoparticle (NP) migration from a poly(ethylene oxide) (PEO) melt into interlamellar region using atomic force microscopy (AFM). Our results confirm the previous hypothesis that NPs migrate into the interlamellar regions at crystallization growth rates smaller than a critical value under isothermal conditions. Under these slow crystallization conditions, bare silica NPs are rejected as defects by the growing crystal of PEO, and the imaging on the large (50 nm) NPs helps track the migration into the amorphous zones. We extend this AFM technique to estimate lamellar growth rates that correlate with spherulite growth rates determined by polarized light optical microscopy (PLOM) but at smaller undercoolings than are typical for PLOM.
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http://dx.doi.org/10.1021/acsmacrolett.1c00778DOI Listing
June 2022

Effects of Long-Term Exposure to Copper on Mitochondria-Mediated Apoptosis in Pig Liver.

Biol Trace Elem Res 2022 Jun 6. Epub 2022 Jun 6.

College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China.

Copper (Cu) is listed as one of the main heavy metal pollutants, which poses potential health risks to humans. Excessive intake of Cu has shown toxic effects on the organs of many animals, and the liver is one of the most important organs to metabolize it. In this study, pigs, the mammal with similar metabolic characteristics to humans, were selected to assess the effects of long-term exposure to Cu on mitochondria-mediated apoptosis, which are of great significance for studying the toxicity of Cu to humans. Pigs were fed a diet with different contents of Cu (10, 125, and 250 mg/kg) for 80 days. Samples of blood and liver tissue were collected on days 40 and 80. Experimental results demonstrated that the accumulation of Cu in the liver was increased in a dose-dependent and time-dependent manner. Meanwhile, the curve of pig's body weight showed that a 125 mg/kg Cu diet promoted the growth of pigs during the first 40 days and then inhibited it from 40 to 80 days, while the 250 mg/kg Cu diet inhibited the growth of pigs during 80 days of feeding. Additionally, the genes and protein expression levels of Caspase-3, p53, Bax, Bak1, Bid, Bad, CytC, and Drp1 in the treatment group were higher than that in the control group, while Bcl-2, Bcl-xL, Opa1, Mfn1, and Mfn2 were decreased. In conclusion, these results indicated that long-term excessive intake of Cu could inhibit the growth of pigs and induced mitochondria-mediated apoptosis by breaking the mitochondrial dynamic balance. Synopsis: Long-term exposure to high doses of Cu could lead to mitochondrial dysfunction by breaking the mitochondrial dynamic balance, which ultimately induced mitochondria-mediated apoptosis in the liver of pigs. This might be closely related to the growth inhibition and liver damage in pigs.
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http://dx.doi.org/10.1007/s12011-022-03303-6DOI Listing
June 2022
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