Publications by authors named "Kai Yu"

678 Publications

Hepcidin-regulating iron metabolism genes and pancreatic ductal adenocarcinoma: a pathway analysis of genome-wide association studies.

Am J Clin Nutr 2021 Jul 13. Epub 2021 Jul 13.

Division of Cancer Epidemiology and Genetics, National Cancer Institute, Rockville, MD, USA.

Background: Epidemiological studies have suggested positive associations for iron and red meat intake with risk of pancreatic ductal adenocarcinoma (PDAC). Inherited pathogenic variants in genes involved in the hepcidin-regulating iron metabolism pathway are known to cause iron overload and hemochromatosis.

Objectives: The objective of this study was to determine whether common genetic variation in the hepcidin-regulating iron metabolism pathway is associated with PDAC.

Methods: We conducted a pathway analysis of the hepcidin-regulating genes using single nucleotide polymorphism (SNP) summary statistics generated from 4 genome-wide association studies in 2 large consortium studies using the summary data-based adaptive rank truncated product method. Our population consisted of 9253 PDAC cases and 12,525 controls of European descent. Our analysis included 11 hepcidin-regulating genes [bone morphogenetic protein 2 (BMP2), bone morphogenetic protein 6 (BMP6), ferritin heavy chain 1 (FTH1), ferritin light chain (FTL), hepcidin (HAMP), homeostatic iron regulator (HFE), hemojuvelin (HJV), nuclear factor erythroid 2-related factor 2 (NRF2), ferroportin 1 (SLC40A1), transferrin receptor 1 (TFR1), and transferrin receptor 2 (TFR2)] and their surrounding genomic regions (±20 kb) for a total of 412 SNPs.

Results: The hepcidin-regulating gene pathway was significantly associated with PDAC (P = 0.002), with the HJV, TFR2, TFR1, BMP6, and HAMP genes contributing the most to the association.

Conclusions: Our results support that genetic susceptibility related to the hepcidin-regulating gene pathway is associated with PDAC risk and suggest a potential role of iron metabolism in pancreatic carcinogenesis. Further studies are needed to evaluate effect modification by intake of iron-rich foods on this association.
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http://dx.doi.org/10.1093/ajcn/nqab217DOI Listing
July 2021

The Value of Different Single or Combined Indexes of the Captopril Challenge Test in the Diagnosis of Primary Aldosteronism.

Front Endocrinol (Lausanne) 2021 17;12:689618. Epub 2021 Jun 17.

Department of Endocrinology and Metabolism, Adrenal Center, Sichuan University West China Hospital, Chengdu, China.

Objective: The result interpretation of the captopril challenge test (CCT) for the diagnosis of primary aldosteronism (PA) is not standardized. Superiorities of different indexes in the CCT have not been fully investigated. We aimed to comprehensively evaluate the value and influence factors of different CCT-associated indexes in the diagnosis of PA.

Methods: We enrolled 312, 85, 179 and 97 patients in the groups of PA, essential hypertension (EH), unilateral PA (UPA) and bilateral PA (BPA), respectively. For each single index investigated, we computed diagnostic estimates including the area under the receiver operating characteristic curve (AUC). We performed pre-specified subgroup analyses to explore influence factors. We assessed the diagnostic value of combined indexes in binary logistic regression models.

Results: Post-CCT aldosterone to renin ratio (ARR) (AUC = 0.8771) and plasma aldosterone concentration (PAC) (AUC = 0.8769) showed high value in distinguishing PA from EH, and their combination (AUC = 0.937) was even superior to either alone. The diagnostic efficacy was moderately high for post-CCT aldosterone to angiotensin II ratio (AA2R) (AUC = 0.834) or plasma renin activity (PRA) (AUC = 0.795) but low for the suppression percentage of PAC (AUC = 0.679). Post-CCT PAC had a significantly higher AUC in the UPA than BPA subgroup (AUC = 0.914 0.827, P<0.05).

Conclusion: We can take post-CCT ARR and PAC altogether into account to distinguish PA from EH, while caution should be taken to interpret CCT results with the suppression percentage of PAC. Post-CCT PAC may perform better to identify the unilateral than bilateral form of PA.
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http://dx.doi.org/10.3389/fendo.2021.689618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247899PMC
June 2021

FTO downregulation mediated by hypoxia facilitates colorectal cancer metastasis.

Oncogene 2021 Jul 3. Epub 2021 Jul 3.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China.

Fat mass and obesity-associated protein (FTO), an N6-methyladenosine (mA) demethylase, participates in tumor progression and metastasis in many malignancies, but its role in colorectal cancer (CRC) is still unclear. Here, we found that FTO protein levels, but not RNA levels, were downregulated in CRC tissues. Reduced FTO protein expression was correlated with a high recurrence rate and poor prognosis in resectable CRC patients. Moreover, we demonstrated that hypoxia restrained FTO protein expression, mainly due to an increase in ubiquitin-mediated protein degradation. The serine/threonine kinase receptor associated protein (STRAP) might served as the E3 ligase and K216 was the major ubiquitination site responsible for hypoxia-induced FTO degradation. FTO inhibited CRC metastasis both in vitro and in vivo. Mechanistically, FTO exerted a tumor suppressive role by inhibiting metastasis-associated protein 1 (MTA1) expression in an mA-dependent manner. Methylated MTA1 transcripts were recognized by an mA "reader", insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2), which then stabilized its mRNA. Together, our findings highlight the critical role of FTO in CRC metastasis and reveal a novel epigenetic mechanism by which the hypoxic tumor microenvironment promotes CRC metastasis.
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http://dx.doi.org/10.1038/s41388-021-01916-0DOI Listing
July 2021

Rapid and efficient method for assessing nanoplastics by an electromagnetic heating pyrolysis mass spectrometry.

J Hazard Mater 2021 Jun 26;419:126506. Epub 2021 Jun 26.

State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, Heilongjiang 150090, PR China; School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, Heilongjiang 150090, PR China; School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, Shandong 264209, PR China. Electronic address:

Nanoplastics are an emerging topic and have attracted increasing attention due to their widespread existence and potential toxicity on living organisms. The challenges of analytical methods for nanoplastics hinder the deeper understanding of toxicological effects and risk assessment of nanoplastics. In this work, a custom-built electromagnetic heating pyrolyzer was coupled to mass spectrometry for the rapid analysis of nanoplastics. Nanoplastics/microplastics were collected on the heat-resisting filter papers, then directly decomposed into gaseous products in the pyrolyzer and analyzed by mass spectrometry. The polystyrene nanoparticles were used to verify the performance of mass-traced quantification, and recoveries of 106-121% and precision of 9% were obtained. As a proof-of-principle experiment, the saline solution packed by polypropylene infusion bottles was aged for simulating indoor sunlight storage, where nanoplastics/microplastics were analyzed. The abundance models of nanoplastics/microplastics in the saline infusion bottle with aging time were assessed from both quality and quantity, for the first time. Results showed that nanoplastics/microplastics in medical infusion products could be generated under indoor sunlight exposure, which needs more attention due to the potential health risks. The proposed electromagnetic heating pyrolysis-mass spectrometry could be a promising method for assessing nanoplastics/microplastics.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126506DOI Listing
June 2021

Identification of antemortem and postmortem fractures in a complex environment by FTIR spectroscopy based on a rabbit tibial fracture self-control model.

Int J Legal Med 2021 Jun 26. Epub 2021 Jun 26.

Department of Forensic Pathology, College of Forensic Medicine, Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China.

The identification of antemortem and postmortem fractures is a critical and challenging task for forensic researchers. Based on our preliminary studies, we explored whether the combination of Fourier transform infrared spectroscopy (FTIR) and chemometrics can identify antemortem and postmortem fractures in complex environments. The impacts of the four environments on the bone spectrum were analyzed by principal component analysis (PCA). It was found that the bone degradation rate in the submerged and ground surface (GS) environments was higher than that in the buried and constant temperature and moisture (CTM) environments. Additionally, the bone degradation rate in buried environment higher than that in the CTM environment. The average spectrum, PCA and partial least squares discriminant analysis (PLS-DA) results all revealed that there were significant differences between the antemortem fracture and the remaining three groups in a complex environment. Compared with the antemortem fracture, the antemortem fracture control (AFC) and postmortem fracture control (PFC) tended to be more similar to the postmortem fracture. According to the loading plot, amide I and amide II were the main components that contributed to the identification of the antemortem fracture, AFC, postmortem fracture, and PFC. Finally, we established a differential model for the antemortem and postmortem fractures (an accuracy of 96.9%), and a differentiation model for the antemortem fracture, AFC, postmortem fracture, and PFC (an accuracy of 87.5%). In conclusion, FTIR spectroscopy is a reliable tool for the identification of antemortem and postmortem fractures in complex environments.
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http://dx.doi.org/10.1007/s00414-021-02633-9DOI Listing
June 2021

Targeted delivery of regulatory macrophages to lymph nodes interferes with T cell priming by preventing the formation of stable immune synapses.

Cell Rep 2021 Jun;35(12):109273

Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany; Cluster of Excellence RESIST (EXC 2155), Hannover Medical School, 30625 Hannover, Germany; German Centre for Infection Research (DZIF), Partner Site Hannover-Braunschweig, 30625 Hannover, Germany. Electronic address:

Immunosuppressive myeloid cells are frequently induced in tumors and attenuate anti-tumor effector functions. In this study, we differentiate immunosuppressive regulatory macrophages (Mregs) from hematopoietic progenitors and test their potential to suppress adaptive immune responses in lymph nodes. Targeted delivery of Mregs to lymph nodes is facilitated by retroviral overexpression of the chemokine receptor CCR7 and intra-lymphatic cell application. Delivery of Mregs completely abolishes the priming of cognate CD8 cells and strongly reduces delayed-type hypersensitivity reactions. Mreg-mediated T cell suppression requires cell-cell contact-regulated nitric oxide production. Two-photon microscopy reveals that nitric oxide produced by Mregs reduces the interaction duration between dendritic cells and T cells. Exposure of activated T cells to nitric oxide strongly reduces their binding to ICAM-1, indicating that nitrosylation of proteins involved in cell adhesion affects synapse formation. Thus, this study identifies a mechanism of myeloid cell-mediated immune suppression and provides an approach for its therapeutic use.
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http://dx.doi.org/10.1016/j.celrep.2021.109273DOI Listing
June 2021

Metabolism and antioxidation regulation of total flavanones from Sedum sarmentosum Bunge against high-fat diet-induced fatty liver disease in Nile tilapia (Oreochromis niloticus).

Fish Physiol Biochem 2021 Aug 18;47(4):1149-1164. Epub 2021 Jun 18.

College of Animal Science and Technology, Guangxi University, No. 100 East Daxue Road, Nanning, 530004, China.

Diet-induced fatty liver is a considerable threaten to fish aquaculture due to the popularity of the high-fat diet (HFD) feeding. Our study aims to investigate the effects of flavanones from Sedum sarmentosum Bunge (FSSB) on the liver function to identify a potential treatment for HFD-induced fatty liver disease. Physiological and pathological indicators were tested in the liver of Nile tilapia (Oreochromis niloticus) and results showed parameters including lipid metabolites, redox parameters, and inflammatory factors could be adequately restored to normal level by addition of 150 mg/kg FSSB to HFD. Proteomics analysis was performed in liver tissues from tilapia with normal diet (ND), HFD, and HFD+FSSB. Totally, 51 upregulated proteins and 77 downregulated proteins were identified in HFD groups and 67 proteins of them were restored after treated with FSSB. Bioinformatics analysis showed that differentially expressed proteins (DEPs) in HFD+FSSB150 group compared with HFD group are mainly enriched in acety-CoA metabolic process, adenosine-triphosphate (ATP) biosynthetic process, lipid metabolic process, and phospholipid metabolic process. The dysregulated proteins were involved in peroxidosome proliferators-activated receptor (PPAR) signaling pathway, fat digestion and absorption, and immune system. The quantitative real-time PCR (qRT-PCR) assay further revealed that the expression of GST, PPARα, PPARγ, and multiple-inflammatory cytokines could be also reversed in HFD group under the treatment of 150 mg/kg FSSB. Our findings demonstrated FSSB is efficient for the treatment of fatty liver disease through regulation of lipid metabolism and antioxidation in Nile tilapia, providing a new treatment of non-alcoholic fatty liver disease (NAFLD) in fish aquaculture.
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http://dx.doi.org/10.1007/s10695-021-00964-3DOI Listing
August 2021

Water Microdroplets Allow Spontaneously Abiotic Production of Peptides.

J Phys Chem Lett 2021 Jun 16;12(24):5774-5780. Epub 2021 Jun 16.

School of Marine Science and Technology, Harbin Institute of Technology at Weihai, Weihai, Shandong 264209, China.

The chemistry of abiotic synthesis of peptides in the context of their prebiotic origins is a continuing challenge that arises from thermodynamic and kinetic constraints in aqueous media. Here we reported a strategy of microdroplets' mass spectrometry for peptide bonds formed from pure amino acids or a mixture in the presence of phosphoric acids in aqueous microdroplets. In contrast to bulk experiments, the condensation reactions proceed spontaneously under ambient conditions. The microdroplet gave a negative free-energy change (Δ ∼ -1.1 kcal/mol), and product yields of ∼75% were obtained at the scale of a few milliseconds. Experiments in which nebulization gas pressure and external charge were varied established dependence of peptide production on the droplet size that has a high surface-to-volume ratio. It is concluded that the condensation reactions occurred at or near the air-water interfaces of microdroplets. This aqueous microdroplets approach also provides a route for chemistry synthesis in the prebiotic era.
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http://dx.doi.org/10.1021/acs.jpclett.1c01083DOI Listing
June 2021

Donor-derived cell-free DNA accurately detects acute rejection in lung transplant patients, a multicenter cohort study.

J Heart Lung Transplant 2021 Aug 24;40(8):822-830. Epub 2021 Apr 24.

Genomic Research Alliance for Transplantation (GRAfT), 10 Center Drive, 7S261, Bethesda, Maryland; Division of Intramural Research, National Heart, Lung and Blood Institute, 10 Center Drive, 7S261, Bethesda, Maryland; Division of Pulmonary and Critical Care Medicine, The Johns Hopkins School of Medicine, 1830 East Monument Street, Baltimore, Maryland. Electronic address:

Background: Acute rejection, which includes antibody-mediated rejection and acute cellular rejection, is a risk factor for lung allograft loss. Lung transplant patients often undergo surveillance transbronchial biopsies to detect and treat acute rejection before irreversible chronic rejection develops. Limitations of this approach include its invasiveness and high interobserver variability. We tested the performance of percent donor-derived cell-free DNA (%ddcfDNA), a non-invasive blood test, to detect acute rejection.

Methods: This multicenter cohort study monitored 148 lung transplant subjects over a median of 19.6 months. We collected serial plasma samples contemporaneously with TBBx to measure %ddcfDNA. Clinical data was collected to adjudicate for acute rejection. The primary analysis consisted of computing the area-under-the-receiver-operating-characteristic-curve of %ddcfDNA to detect acute rejection. Secondary analysis determined %ddcfDNA rule-out thresholds for acute rejection.

Results: ddcfDNA levels were high after transplant surgery and decayed logarithmically. With acute rejection, ddcfDNA levels rose six-fold higher than controls. ddcfDNA levels also correlated with severity of lung function decline and histological grading of rejection. %ddcfDNA area-under-the-receiver-operating-characteristic-curve for acute rejection, AMR, and ACR were 0.89, 0.93, and 0.83, respectively. ddcfDNA levels of <0.5% and <1.0% showed a negative predictive value of 96% and 90% for acute rejection, respectively. Histopathology detected one-third of episodes with ddcfDNA levels ≥1.0%, even though >90% of these events were coincident to clinical complications missed by histopathology.

Conclusions: This study demonstrates that %ddcfDNA reliably detects acute rejection and other clinical complications potentially missed by histopathology, lending support to its use as a non-invasive marker of allograft injury.
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http://dx.doi.org/10.1016/j.healun.2021.04.009DOI Listing
August 2021

Different Changing Patterns of Three NOS-NO System Activities after Ischemia-Reperfusion in Rabbit with AMI.

Cell Biochem Biophys 2021 Jun 14. Epub 2021 Jun 14.

Department of Cardiology, CangZhou Central Hospital, CangZhou, Hebei, China.

NOS-NO system activity is closely correlated with ischemia-reperfusion injury (IRI) and NOS subtypes were suggested to play different roles in IRI. In this work, the activity of serum NOS, NO levels, and ischemic necrosis after reperfusion in rabbit with AMI at different time was studied. We also explored the NOS-NO system activity changes and its correlation with myocardial ischemia and necrosis. It shows that after reperfusion in rabbits with AMI, NO-NOS system activities present different changes at each time point due to inactivation of NO and iNOS activation, and different experimental animals, ischemia-reperfusion degree, and length of time will also lead to different research results. Therefore, it is necessary to conduct dynamic observation on animals from different species at multi-temporal point under the state of NOS-NO system activities, and simultaneously detect inflammatory factor, MDA, and SOD indexes. Therefore, it is a must to conduct relevant drug research studies to make NOS-NO system activities maintain the level in favor of ideal myocardial ischemia reperfusion.
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http://dx.doi.org/10.1007/s12013-021-01011-xDOI Listing
June 2021

Wells-Dawson Arsenotungstate Porous Derivatives for Electrochemical Supercapacitor Electrodes and Electrocatalytically Active Materials.

Inorg Chem 2021 Jul 14;60(13):9869-9879. Epub 2021 Jun 14.

Key Laboratory for Photonic and Electronic Bandgap Materials, Ministry of Education, School of Chemistry and Chemical Engineering, Harbin Normal University, Harbin 150025, P. R. China.

Two Wells-Dawson arsenotungstate coordination polymers, [{Cu(bim)}(AsWO)] () and [(CupzCl)(AsWO)] (bim = 2,2'-biimidazole; pz = pyrazine), have been assembled via a hydrothermal method and fully characterized. Compound exhibits a 2,6-connected two-dimensional hybrid layer based on asymmetrically modified {AsW} anions and {Cu(bim)} linkers, which is extended to a three-dimensional network with a special interlayer structure and a one-dimensional tunnel. Compound is a host-guest framework that consists of a Cu-pz-Cl network with 20-member square rings, 16-member irregular rings, and embedded eight-node {AsW} guest molecules. Compounds and show uncommon specific capacitance (834.8 and 960.1 F g, respectively, at a current density of 2.4 A g), enduring cycling stability (capacitance retention rates of 89.3% and 91.9%, respectively, after 5000 cycles), and good electrical conductivity, which are superior to those of the unmodified zero-dimensional Dawson arsenotungstate compound and most reported electrode materials in terms of their stable structure, special layer spacing, and orderly channels. Moreover, the title compounds exhibit excellent electrocatalytic activity for oxidizing ascorbic acid and reducing nitrite.
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http://dx.doi.org/10.1021/acs.inorgchem.1c01136DOI Listing
July 2021

A new Cu(II)-containing coordination polymer: protective effect and mechanism exploration on elderly osteoporotic hip fractures via regulating the wnt signaling pathway.

Mol Divers 2021 Jun 9. Epub 2021 Jun 9.

Department of Medicine, Soochow University, Changzhou, Jiangsu, China.

Elderly osteoporosis hip fracture has drawn the attention of the researcher. The coordination polymer was now widely used in clinic because their multiple structures and biological activities. In this present research, the new coordination polymer was designed and synthesized and their application values on elderly osteoporosis hip were evaluated. The reaction between Cu(II) salt and 4,4'-(1H-1,2,4-triazol-1-yl)methylene-bis(benzoic acid) (Htzmb), an aromatase inhibitor letrozole derivative with the aid of the organic linker 4,4-bipyridine (4,4'-bpy) affords a new coordination polymer based on Cu(II) ions as nodes of {[Cu(tzmb)(4,4'-bpy) (μ-HO)(DMA) (HO)]·10DMA} (1). The complex 1 gained is totally investigated with the powder X-ray diffraction study, thermogravimetric analyses, the diffraction of single-crystal X-ray, elemental analysis as well as the Fourier transform infrared spectrometer spectra. The osteogenic differentiation of mesenchymal stem cell was measured with the western blotting assay through determining the Runx2 expression. The wnt signaling pathway relative expression in mesenchymal stem cell was detected through the determination of real-time RT-PCR. The cytotoxicity of the compound on the mesenchymal stem cell was determined with CCK-8 assay. The result of single-crystal X-ray diffraction reflects that the complex 1 has crystallized in space group R-3c of trigonal system and exhibits a three-dimensional skeleton architecture on the bases of the SBUs {Cu(tzmb)(μ-HO)}. The western blotting assay results revealed that this compound could significantly promote the osteogenic differentiation of mesenchymal stem cell. Besides, the activation of wnt signaling pathway in mesenchymal stem cell was also increased by the compound exposure. Finally, it can be summed up that this compound has excellent application values on the elderly osteoporotic hip fractures treatment by increasing the activation of wnt signaling pathway in mesenchymal stem cell. The results of the CCK-8 assay indicated that the compound has no cytotoxicity on the mesenchymal stem cell. The molecular docking simulation results have identified that only the carboxyl group on the Cu complex exhibits the activity for the hydrogen bond formation, however, the pyridine ring does not have such activity, instead, the pyridine ring only acts as the ligand that binds to the Cu ion. This Cu(II) coordination polymer has excellent application values on the elderly osteoporotic hip fractures treatment by increasing the activation of wnt signaling pathway in mesenchymal stem cell.
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http://dx.doi.org/10.1007/s11030-021-10241-3DOI Listing
June 2021

LncRNA HAS2-AS1 Promotes Glioblastoma Proliferation by Sponging miR-137.

Front Oncol 2021 20;11:634893. Epub 2021 May 20.

Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China.

GBM (Glioblastoma multiform) is the most malignant tumor type of the central nervous system and has poor diagnostic and clinical outcomes. LncRNAs (Long non-coding RNAs) have been reported to participate in multiple biological and pathological processes, but their underlying mechanism remains poorly understood. Here, we aimed to explore the role of the lncRNA HAS2-AS1 (HAS2 antisense RNA 1) in GBM. GSE103227 was analyzed, and qRT-PCR was performed to measure the expression of HAS2-AS1 in GBM. FISH (Fluorescence hybridization) was performed to verify the localization of HAS2-AS1. The interaction between HAS2-AS1 and miR-137 (microRNA-137) was predicted by LncBook and miRcode followed by dual-luciferase reporter assays, and the relationships among HAS2-AS1, miR-137 and LSD1 (lysine-specific demethylase 1) were assessed by WB (western blot) and qRT-PCR. Colony formation and CCK-8 (cell counting kit-8) assays were performed as functional tests. In vivo, nude mice were used to confirm the function of HAS2-AS1. HAS2-AS1 expression was upregulated in GBM cell lines, and HAS2-AS1 was localized mainly in the cytoplasm. , high HAS2-AS1 expression promoted proliferation, and knockdown of HAS2-AS1 significantly inhibited proliferation. Furthermore, HAS2-AS1 functioned as a ceRNA (competing endogenous RNA) of miR-137, leading to the disinhibition of its downstream target LSD1. The miR-137 level was downregulated by HAS2-AS1 overexpression and upregulated by HAS2-AS1 knockdown. In a subsequent study, LSD1 expression was negatively regulated by miR-137, while miR-137 reversed the LSD1 expression levels caused by HAS2-AS1. These results were further supported by the nude mouse tumorigenesis experiment; compared with xenografts with high HAS2-AS1 expression, the group with low levels of HAS2-AS1 exhibited suppressed proliferation and better survival. We conclude that lncRNA HAS2-AS1 promotes proliferation by functioning as a miR-137 decoy to increase LSD1 levels and thus might be a possible biomarker for GBM.
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http://dx.doi.org/10.3389/fonc.2021.634893DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173206PMC
May 2021

Synthesis of a Co-Sn Alloy-Deposited PTFE Film for Enhanced Solar-Driven Water Evaporation via a Super-Absorbent Polymer-Based "Water Pump" Design.

ACS Appl Mater Interfaces 2021 Jun 2;13(23):26879-26890. Epub 2021 Jun 2.

Key Laboratory of Photochemical Biomaterials and Energy Storage Materials, College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin, Heilongjiang 150025, China.

Solar-driven water evaporation is a promising solution to water pollution, the energy crisis, and water shortages. However, the approach in which the photothermal film is in direct contact with bulk water for water evaporation may lead to a large amount of heat loss, thereby reducing the light-to-heat conversion efficiency (η) of the photothermal film. Here, a highly efficient solar-driven water evaporation system was developed using a Co-Sn alloy-deposited Teflon (PTFE) film (Co-Sn [email protected]) and super-absorbent polymers (SAPs) supported with a floating foam substrate. The Co-Sn alloy with full-spectrum (200-2500 nm) absorption characteristics is devoted to high light-to-heat conversion, while the porous PTFE with high mechanical performance can support the Co-Sn alloy. We used density functional theory to prove that the Co-Sn alloy had a strong adhesive force with PTFE without surfactants due to the high adsorption energy between the (101) crystal plane of the Co-Sn alloy and the hydroxyl group on the PTFE film. Importantly, via the SAP-based "water pump" design, we improved the η of the Co-Sn [email protected] film to 89%, mainly because the SAP not only effectively performed water transportation but also markedly reduced the heat loss of the Co-Sn [email protected] film. Our work highlights the strong potential of Co-Sn [email protected] light-to-heat conversion systems for realizing highly effective solar energy-driven water evaporation.
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http://dx.doi.org/10.1021/acsami.1c02586DOI Listing
June 2021

Malnutrition in Relation with Dietary, Geographical, and Socioeconomic Factors among Older Chinese.

Biomed Environ Sci 2021 May;34(5):337-347

National Institute of Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China.

Objective: Nutrition is closely related to the health of the elderly population. This study aimed to provide a comprehensive picture of the nutrition status of elderly Chinese and its related dietary, geographical, and socioeconomic factors.

Methods: A total of 13,987 ≥ 60-year-old persons from the 2010-2013 Chinese National Nutrition and Health Survey were included to evaluate various aspects of malnutrition, including underweight, overweight or obesity, and micronutrient inadequacy.

Results: Overall, the prevalence of obesity, overweight, and underweight was 12.4%, 34.8%, and 5.7%, respectively, with disparities both geographically and socioeconomically. The prevalence of underweight was higher among the older old (≥ 75 years), rural residents and those with low income, with low education status, and residing in undeveloped West areas. More than 75% of the elderly do not meet the Dietary Reference Intakes for vitamins A, B , B , and E, folate, calcium, selenium, potassium, biotin, and choline, with the prevalence of inadequate intake increasing with age for most nutrients. At the population level, the mean intakes of numerous food groups did not meet the recommendations by the Chinese Dietary Guideline.

Conclusions: Obesity epidemic, inadequacy of micronutrient intake, and high prevalence of underweight and anemia in susceptible older people are the major nutrition challenges for the rapidly aging population in China.
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http://dx.doi.org/10.3967/bes2021.045DOI Listing
May 2021

miR-451 suppresses EMT and metastasis in glioma cells.

Cell Cycle 2021 May 28:1-9. Epub 2021 May 28.

Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China.

The metastasis of tumor cells is a challenge for the clinical treatment of glioma. Epithelial-mesenchymal transition (EMT) contributes to glioma cell invasiveness. Our previous study confirmed that the expression of miRNA-451, which inhibits the PI3K/Akt signaling pathway by directly targeting CAB39 and plays a repressive role in glioma, is downregulated in glioma. However, the specific mechanism of miRNA-451 regulation in glioma is unclear. In this study, we investigated whether miRNA-451 blocks the processes of EMT and metastasis in glioma cells in vivo and in vitro. By targeting CAB39, miRNA-451 likely triggers the PI3K/Akt/Snail signaling pathway to reduce glioma proliferation, invasion, migration and EMT. We used Western blotting experiments to demonstrate that overexpression of miRNA-451 significantly reduced p-AKT(Ser473), N-cadherin, Vimentin, Twist, Snail and Cyclin D1 expression and increased E-cadherin expression. We demonstrated that overexpression of miR-451 suppressed glioma cell proliferation, invasion, migration and EMT by MTT and colony formation assays, Transwell assays, wound healing assays and animal experiments. Taken together, these results suggest that miRNA-451 can reduce EMT and metastasis in glioma cells through the suppression of the PI3K/Akt/Snail signaling pathway by targeting CAB39 in vitro and in vivo. miR-451 may be a new target for glioma treatment.
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http://dx.doi.org/10.1080/15384101.2021.1933303DOI Listing
May 2021

Non-stoichiometric cobalt sulfide nanodots enhance photothermal and chemodynamic therapies against solid tumor.

J Colloid Interface Sci 2021 Oct 13;600:390-402. Epub 2021 May 13.

Key Laboratory of Photochemical Biomaterials and Energy Storage Materials, College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin, Heilongjiang 150025, China. Electronic address:

Photodynamic therapy (PDT) mainly relies on reactive oxygen species generated by light- activated photosensitizers and oxygen to kill tumor cells. However, a critical limitation of the current PDT is that it is less effective in solid tumors where the microenvironment is hypoxic, and, therefore, repeated treatment is required. Here, non-stoichiometric CoS nanodots (NDs), which can be rapidly degraded to cobalt (Co) and sulfur (S) ions, were developed to enhance tumor photothermal therapy (PTT) and chemodynamic therapy (CDT) via the capture of copper (Cu) ions (starvation therapy) in the hypoxic tumor microenvironment under near-infrared irradiation. CoS NDs with excellent photothermal conversion efficiency (ɳ = 52%) can be used for PTT, and the Co ions produced by their degradation can catalyze the endogenous hydrogen peroxide of tumor cells to produce highly toxic hydroxyl radicals to achieve tumor CDT. The mechanism of starvation therapy was explored using western blotting, and the results indicated that blocking the uptake of Cu ions could restrain the growth and proliferation of tumors by inhibiting the BRAF/mitogen-activated extracellular signal regulated kinase (MEK)/extracellular regulated protein kinases (ERK) signaling pathway. Our work highlights the great potential of CoS NDs as a theranostic agent for implementing photoacoustic/photothermal imaging and starvation therapy-enhanced PTT/CDT.
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http://dx.doi.org/10.1016/j.jcis.2021.05.058DOI Listing
October 2021

ABO genotypes and the risk of esophageal and gastric cancers.

BMC Cancer 2021 May 22;21(1):589. Epub 2021 May 22.

Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, 9609 Medical Center Dr. 6E3280, Rockville, MD, 20850, USA.

Background: Blood type has been associated with the risk of gastric cancer, but few studies have examined the association with esophageal squamous cell carcinoma (ESCC).

Methods: We conducted a case-control study using genotyping data of Chinese individuals, including cases of 2022 ESCC, 1189 gastric cardia adenocarcinoma, 1161 gastric noncardia adenocarcinoma, and 2696 controls. Genetic blood type was imputed using three single nucleotide polymorphisms. We used logistic regression to examine the association between blood type and the risk of each cancer.

Results: Compared to blood type O, the risk of ESCC was significantly elevated for blood type B and AB, with the highest risk for type AB (OR, 95%CI: 1.34, 1.07-1.67). Analysis of genotype suggested that the association of ESCC was from carrying the B allele. Similarly, blood type was significantly associated with gastric noncardia adenocarcinoma (P < 0.001) with risk significantly elevated in type A (1.37, 1.14-1.65) and AB (1.44, 1.10-1.89) compared to type O. Blood type was not associated with gastric cardia adenocarcinoma (P = 0.13).

Conclusions: This study provides novel insights into the association between blood type and the risk of ESCC and restricted previously observed association to only gastric noncardia cancer, providing important evidence to clarify the pattern of association and suggesting mechanisms of action.
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http://dx.doi.org/10.1186/s12885-021-08334-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8141232PMC
May 2021

CuSe/[email protected] Z-scheme heterostructure: a multimode bioimaging guided theranostic agent with enhanced photo/chemodynamic and photothermal therapy.

Biomater Sci 2021 Jun;9(12):4473-4483

Key Laboratory of Photochemical Biomaterials and Energy Storage Materials and College of Chemistry and Chemical Engineering, Harbin Normal University, Harbin 150025, China.

Photodynamic therapy (PDT) can be defined as a kind of intracellular photocatalysis. Inspired by the design of photocatalysts, the construction of the heterojunction also is expected to improve the production of reactive oxygen species (ROS) for PDT. Herein, the Cu2-xSe/[email protected] ([email protected]) nano-heterostructure has been prepared by a cation-exchange process, where the interaction between the host and exchange agent is vital. [email protected] exhibits the near-infrared (NIR)-triggered hydroxyl radical and singlet oxygen (˙OH and 1O2) generation, which is more than 6 times in contrast with that of pure [email protected], attributed to the Z-scheme charge transfer mechanism with the high redox ability and great charge separation. Moreover, with the narrower band gap of Bi2Se3, [email protected] exhibits enhanced NIR harvest as well as high photothermal conversion efficiency (60.4%). Due to the Fenton reaction caused by the Cu ion, [email protected] is endowed with the chemodynamic therapy (CDT) and signal-enhanced T1-weight magnetic resonance imaging (MRI) capacity. In addition, the great photothermal ability and X-ray absorption coefficient provide outstanding contrast in photothermal imaging (PTI) and computerized tomography (CT) imaging. Finally, the multi-imaging combined with the synergistic treatment (PTT/CDT/PDT) makes [email protected] achieve enhanced efficiency in anticancer therapy.
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http://dx.doi.org/10.1039/d1bm00378jDOI Listing
June 2021

DNA methylation regulator-mediated modification patterns and tumor microenvironment characterization in gastric cancer.

Mol Ther Nucleic Acids 2021 Jun 3;24:695-710. Epub 2021 Apr 3.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou 510060, P.R. China.

Growing evidence implies a link between DNA methylation and tumor immunity/immunotherapy. However, the global influence of DNA methylation on the characteristics of the tumor microenvironment and the efficacy of immunotherapy remains to be clarified. In this study, we systematically evaluated the DNA methylation regulator patterns and tumor microenvironment characteristics of 1,619 gastric cancer patients by clustering the gene expression of 20 DNA methylation regulators. Three gastric cancer subtypes that had different DNA methylation modification patterns and distinct tumor microenvironment characteristics were recognized. Then, a DNA methylation score (DMS) was constructed to evaluate DNA methylation modification individually. High DMS was characterized by immune activation status, increased tumor mutation burden, and tumor neoantigens, with a favorable prognosis. Conversely, activation of the stroma and absence of immune cell infiltration were observed in the low DMS group, with relatively poor survival. High DMS was also certified to be correlated with enhanced efficacy of immunotherapy in four immune checkpoint blocking treatment cohorts. In conclusion, the characterization of DNA methylation modification patterns may help to enhance our recognition of the tumor immune microenvironment of gastric cancer and guide more personalized immunotherapy strategies in the future.
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http://dx.doi.org/10.1016/j.omtn.2021.03.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099484PMC
June 2021

Neoantigen landscape in metastatic nasopharyngeal carcinoma.

Theranostics 2021 19;11(13):6427-6444. Epub 2021 Apr 19.

Department of Nasopharyngeal Carcinoma, Sun Yat-sen University Cancer Center, 651 Dongfeng East Road, Guangzhou 510060, P. R. China.

Reportedly, nasopharyngeal carcinoma (NPC) patients with MHC I Class aberration are prone to poor survival outcomes, which indicates that the deficiency of tumor neoantigens might represent a mechanism of immune surveillance escape in NPC. To clearly delineate the landscape of neoantigens in NPC, we performed DNA and RNA sequencing on paired primary tumor, regional lymph node metastasis and distant metastasis samples from 26 patients. Neoantigens were predicted using pVACseq pipeline. Subtype prediction model was built using random forest algorithm. Portraying the landscape of neoantigens in NPC for the first time, we found that the neoantigen load of NPC was above average compared to that of other cancers in The Cancer Genome Atlas program. While the quantity and quality of neoantigens were similar among primary tumor, regional lymph node metastasis and distant metastasis samples, neoantigen depletion was more severe in metastatic sites than in primary tumors. Upon tracking the clonality change of neoantigens, we found that neoantigen reduction occurred during metastasis. Building a subtype prediction model based on reported data, we observed that subtype I lacked T cells and suffered from severe neoantigen depletion, subtype II highly expressed immune checkpoint molecules and suffered from the least neoantigen depletion, and subtype III was heterogenous. These results indicate that neoantigens are conducive to the guidance of clinical treatment, and personalized therapeutic vaccines for NPC deserve deeper basic and clinical investigations to make them feasible in the future.
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http://dx.doi.org/10.7150/thno.53229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120206PMC
July 2021

Fourier-transform infrared microspectroscopy of pulmonary edema fluid for postmortem diagnosis of diabetic ketoacidosis.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Sep 26;258:119882. Epub 2021 Apr 26.

Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Ministry of Justice, No. 1347 West Guangfu Rd., Shanghai 200063, China. Electronic address:

Determination of the cause of death for diabetic ketoacidosis (DKA), a common and fatal acute complication of diabetes mellitus, is a challenging forensic task owing to the lack of characteristic morphological findings at autopsy. In this study, Fourier-transform infrared (FTIR) microspectroscopy coupled with chemometrics was employed to characterize biochemical differences in pulmonary edema fluid from different causes of death to supplement conventional methods and provide an efficient postmortem diagnosis of DKA. With this aim, FTIR spectra in three different situations (DKA-caused death, other causes of death with diabetes history, and other causes of death without diabetes history) were measured. The results of principal component analysis indicated different spectral profiles between these three groups, which mainly exhibited variations in proteins. Subsequently, two binary classification models were established using an algorithm of partial least squares discriminant analysis (PLS-DA) to determine whether decedents had diabetes and whether the diabetic patients died from DKA. Satisfactory prediction results of PLS-DA models demonstrated good differentiation among these three groups. Therefore, it is feasible to make a postmortem diagnosis of DKA and detect diabetes history via FTIR microspectroscopic analysis of the pulmonary edema fluid.
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http://dx.doi.org/10.1016/j.saa.2021.119882DOI Listing
September 2021

Intrinsic functional neuron-type selectivity of transcranial focused ultrasound neuromodulation.

Nat Commun 2021 05 4;12(1):2519. Epub 2021 May 4.

Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.

Transcranial focused ultrasound (tFUS) is a promising neuromodulation technique, but its mechanisms remain unclear. We hypothesize that if tFUS parameters exhibit distinct modulation effects in different neuron populations, then the mechanism can be understood through identifying unique features in these neuron populations. In this work, we investigate the effect of tFUS stimulation on different functional neuron types in in vivo anesthetized rodent brains. Single neuron recordings were separated into regular-spiking and fast-spiking units based on their extracellular spike shapes acquired through intracranial electrophysiological recordings, and further validated in transgenic optogenetic mice models of light-excitable excitatory and inhibitory neurons. We show that excitatory and inhibitory neurons are intrinsically different in response to ultrasound pulse repetition frequency (PRF). The results suggest that we can preferentially target specific neuron types noninvasively by tuning the tFUS PRF. Chemically deafened rats and genetically deafened mice were further tested for validating the directly local neural effects induced by tFUS without potential auditory confounds.
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http://dx.doi.org/10.1038/s41467-021-22743-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097024PMC
May 2021

Preparation of a polyclonal antibody against the non-structural protein, NSs of SFTSV.

Protein Expr Purif 2021 Aug 23;184:105892. Epub 2021 Apr 23.

Department of Pathogenic Microbiology, Jinzhou Medical University, Jinzhou, China. Electronic address:

Severe fever with thrombocytopenia syndrome virus (SFTSV) is newly discovered virus which is the member of the order Bunyavirales, family phenuiviridae, phlebovirus genus. Its genome is composed of 3 segments of negative-sense RNA L, M and S. NSs is a non structure protein encoded by S segment which is important for viral replication and virulence. NSs protein of SFTSV is only involved in the regulation of host innate immune responses and suppression of IFN-promoter activities. So, the exact functions of this protein need to be studied deeply. To understand the exact role of NSs from SFTSV in viral replication and host immune response, a qualified antibody against this protein is required. In this study, NSs gene of SFTSV, was cloned into a bacterial expression vector (pGEX-6P-1) and the recombinant plasmid was transformed into Escherichia coli BL21 (DE3) cells. The SFTSV NSs fusion protein was purified using Glutathione Sepharose 4B and utilized as an antigen to immunize rabbits and obtain an anti-SFTSV NSs polyclonal antibody. Proper expression of the fusion protein and polyclonal antibody specificity were confirmed by western blotting and immunofluorescence analyses. The polyclonal antibody recognized NSs from SFTSV specifically. This is the first report that NSs can form viroplasm-like structures not only in infected cells but also in transfected cells with NSs plasmids. This polyclonal antibody will be useful for future studies of NSs functions.
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http://dx.doi.org/10.1016/j.pep.2021.105892DOI Listing
August 2021

Speckle Noise Reduction for OCT Images based on Image Style Transfer and Conditional GAN.

IEEE J Biomed Health Inform 2021 Apr 21;PP. Epub 2021 Apr 21.

Raw optical coherence tomography (OCT) images typically are of low quality because speckle noise blurs retinal structures, severely compromising visual quality and degrading performances of subsequent image analysis tasks. In our previous study, we have developed a Conditional Generative Adversarial Network (cGAN) for speckle noise removal in OCT images collected by several commercial OCT scanners, which we collectively refer to as scanner T. In this paper, we improve the cGAN model and apply it to our in-house OCT scanner (scanner B) for speckle noise suppression. The proposed model consists of two steps: 1) We train a Cycle-Consistent GAN (CycleGAN) to learn style transfer between two OCT image datasets collected by different scanners. The purpose of the CycleGAN is to leverage the ground truth dataset created in our previous study. 2) We train a mini-cGAN model based on the PatchGAN mechanism with the ground truth dataset to suppress speckle noise in OCT images. After training, we first apply the CycleGAN model to convert raw images collected by scanner B to match the style of the images from scanner T, and subsequently use the mini-cGAN model to suppress speckle noise in the style transferred images. We evaluate the proposed method on a dataset collected by scanner B. Experimental results show that the improved model outperforms our previous method and other state-of-the-art models in speckle noise removal, retinal structure preservation and contrast enhancement.
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http://dx.doi.org/10.1109/JBHI.2021.3074852DOI Listing
April 2021

A Novel Diagnostic Model for Primary Adrenal Lymphoma.

Front Endocrinol (Lausanne) 2021 2;12:636658. Epub 2021 Apr 2.

Adrenal Center, Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, China.

Objective: Primary adrenal lymphoma (PAL) is easily misdiagnosed as other adrenal masses, such as adrenocortical carcinoma and pheochromocytoma, but patients with PAL benefit little from surgery. The diagnostic method for PAL thus far is limited to adrenal biopsy. In our study, we aimed to develop a quick and efficient diagnostic method for PAL.

Methods And Results: At the same institution, 505 patients (between 2009 and 2019) and 171 patients (between 2019 and 2020) were separately included in the primary and validation studies. Univariate and multivariate analyses were conducted to evaluate clinical manifestations, laboratory findings, and radiological characteristics. Four determinants (age, bilateral masses, high-density lipoprotein cholesterol, and lactate dehydrogenase) were selected and further incorporated into a regression model to screen PAL. Accordingly, the nomogram was developed for clinical practice. In the primary study, the nomogram showed good discrimination, with an area under the receiver operating characteristic (ROC) curve (AUC) of 95.4% (95% CI, 90.6%-100.0%). Further validation study verified the efficacy of the nomogram, with an AUC of 99.0% (95% CI, 96.9%-100.00%) and 100.0% in all patients and patients with bilateral masses, respectively, and a sensitivity/specificity/positive predictive value (PPV)/negative predictive value (NPV) of 66.67%/99.40%/66.67%/99.40%, 66.67%/100%/100%/92.86%, 50%/99.20%/50%/99.20%, and 100%/100%/100%/100%, in all patients, patients with bilateral adrenal masses, patients with nonfunctional adrenal masses, and patients with positive catecholamine results, respectively. The validation study also revealed a diagnostic specificity of 99.35% and 100% for patients with a unilateral adrenal mass and functional PCC, respectively.

Conclusions: The presented nomogram is the first user-friendly diagnostic model for PAL that simplifies the complex diagnostic process into personalized numeric estimates. We deem that patients who score below 50 are less likely to have PAL. We suggest that clinicians should arrange adrenal biopsy and surgery for patients with nonfunctional tumors and overt catecholamine-secreting tumors, respectively, who receive a score of 50 points or higher to confirm the diagnosis as soon as possible.
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http://dx.doi.org/10.3389/fendo.2021.636658DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8050348PMC
April 2021

Irisin enhances osteogenic differentiation of mouse MC3T3-E1 cells via upregulating osteogenic genes.

Exp Ther Med 2021 Jun 31;21(6):580. Epub 2021 Mar 31.

Department of Orthopaedics, Peking University People's Hospital, Beijing 10000, P.R. China.

Osteoporosis affects millions of individuals and remains a clinical challenge in terms of prevention and treatment. The present study aimed to investigate the effect of irisin on osteogenic differentiation by exposing MC3T3-E1 cells to different concentrations of irisin. Treated cells were assayed for osteoblast proliferation and osteogenic differentiation by measuring alkaline phosphatase (ALP) activity, calcium deposition, formation of mineralized nodules and the expression of osteogenic genes using reverse transcription-quantitative PCR. The proliferation of MC3T3-E1 cells was unaffected by irisin at the concentrations tested of up to 100 ng/ml (P>0.05). ALP activity and mineralized nodule formation were significantly enhanced by irisin in a dose- and time-dependent manner, indicating that irisin promotes osteoblast differentiation of MC3T3-E1 cells. The expression of osteogenic genes, including ALP, collagen I, runt-related transcription factor 2, osterix, osteopontin, osteocalcin, osteoprotegerin and estrogen receptor α, increased significantly after irisin treatment. The present study demonstrated that irisin promoted the osteogenic differentiation of MC3T3-E1 cells, possibly by upregulating the expression of osteogenic genes and markers. Therefore, irisin may be worthy of further investigation as a potential therapeutic agent for osteoporosis.
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http://dx.doi.org/10.3892/etm.2021.10012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027760PMC
June 2021

Epidemiological characteristics of 561 cases of intracerebral hemorrhage in Chengdu, China.

Medicine (Baltimore) 2021 Apr;100(15):e24952

Department of Neurosurgery.

Abstract: To explore the epidemiology of patients with spontaneous intracerebral hemorrhage (sICH) in Chengdu, China, we retrieved the data of patients with spontaneous cerebral hemorrhage admitted to the First Affiliated Hospital of Chengdu Medical College from January 2017 to December 2019. We performed a comprehensive analysis of the location of hemorrhage, demographics, factors of hemorrhage, condition of body, severity of disturbance of consciousness, treatment, length of stay (days), inpatient costs, prognosis, and mortality rate in patients with sICH. In total, data of 561 in patients with sICH were included. The hemorrhage site was primarily located in the basal ganglia and thalamus (64.71%). The mean patient age was 63.2 ± 12.4 years (64.17% men, 35.83% women). Male patients (mean age 62.3 ± 12.5 year) were younger than female patients (mean age 64.9 ± 12.1 year). The age of sICH onset in our sample was between 40 and 79 years; this occurred in 87.70% of the included cases. There were more males than females, which may be related to more daily smoking, longer drinking years, and overweight in males than in females. Cases occurred most frequently during the winter and spring months, and the relationship between sICH visits and hospitalizations appeared as a U-shape. The median time from illness onset to hospital admission was 3.0 hours. According to the Glasgow Coma Scale (GCS) score at admission, 20.50% of sICH cases were of mild intensity, 39.93% were moderate, and 39.57% were severe. Moderate disorder is the most common sICH severity. Factors influencing the disturbance of consciousness were blood glucose level at the time of admission as well as the number of years with hypertension. The lower the degree of disturbance of consciousness and the more they smoked per day indicated they had a higher likelihood of receiving surgical treatment while in hospital. The median hospital stay was 13.0 days, while the median inpatient cost was USD 3609. The 30-day mortality rate was 18.36%. sICH is an important public health problem in Chengdu, China. A governmental initiative is urgently needed to establish a sICH monitoring system that covers the Chengdu region to develop more effective and targeted measures for sICH prevention, treatment, and rehabilitation.
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http://dx.doi.org/10.1097/MD.0000000000024952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8052055PMC
April 2021

Integrative analysis of multiple case-control studies.

Biometrics 2021 Mar 25. Epub 2021 Mar 25.

Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, Maryland, USA.

It is often challenging to share detailed individual-level data among studies due to various informatics and privacy constraints. However, it is relatively easy to pool together aggregated summary level data, such as the ones required for standard meta-analyses. Focusing on data generated from case-control studies, we present a flexible inference procedure that integrates individual-level data collected from an "internal" study with summary data borrowed from "external" studies. This procedure is built on a retrospective empirical likelihood framework to account for the sampling bias in case-control studies. It can incorporate summary statistics extracted from various working models adopted by multiple independent or overlapping external studies. It also allows for external studies to be conducted in a population that is different from the internal study population. We show both theoretically and numerically its efficiency advantage over several competing alternatives.
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http://dx.doi.org/10.1111/biom.13461DOI Listing
March 2021
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