Publications by authors named "Kai Xu"

1,044 Publications

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Circ_0061140 facilitates adenomyosis progression by up-regulating LIN28B in vitro.

Gynecol Obstet Invest 2022 Aug 10. Epub 2022 Aug 10.

Objectives: The aim of our study was to explore the role of circular RNA_0061140 (circ_0061140) in adenomyosis progression and its associated mechanism.

Design: We first analyzed the expression pattern of circ_0061140 in endometrial tissues of adenomyosis patients (n=27) and uterine fibroids patients (n=15). Loss-of-function experiments were conducted to analyze the biological roles of circ_0061140 in regulating the viability, apoptosis, proliferation, migration, and invasion of endometrial epithelial cells. The downstream microRNA (miRNA)/messenger RNA (mRNA) axis of circ_0061140 was predicted by bioinformatics tool Starbase, and its working mechanism was verified by rescue experiments.

Methods: Cell viability, apoptosis, proliferation, invasion, and migration were assessed by Cell Counting Kit-8 (CCK8) assay, flow cytometry (FCM) analysis, 5-Ethynyl-2'-deoxyuridine (EdU) assay, transwell assay, and scratch test. The binding relationship between miR-141-3p and circ_0061140 or lin-28 homolog B (LIN28B) was verified by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay.

Results: Circ_0061140 expression was up-regulated in adenomyosis patients. Circ_0061140 knockdown suppressed the viability, proliferation, invasion, and migration and triggered the apoptosis of endometrial epithelial cells. Circ_0061140 served as a miRNA sponge for miR-141-3p, and miR-141-3p silencing partly reversed circ_0061140 knockdown-induced effects in endometrial epithelial cells. miR-141-3p directly interacted with LIN28B mRNA. LIN28B overexpression partly diminished miR-141-3p overexpression-mediated influences in endometrial epithelial cells. Circ_0061140 knockdown down-regulated LIN28B expression by elevating miR-141-3p level in endometrial epithelial cells.

Limitations: The functional verification of circ_0061140/miR-141-3p/LIN28B axis was merely conducted in vitro.

Conclusion: In conclusion, circ_0061140 contributed to adenomyosis progression by binding to miR-141-3p to induce LIN28B expression in vitro.
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http://dx.doi.org/10.1159/000525889DOI Listing
August 2022

Retrospective Analysis of Switching Bimatoprost 0.01% to Bimatoprost 0.03% in Patients with Various Types of Glaucoma and Ocular Hypertension.

Clin Ophthalmol 2022 29;16:2385-2390. Epub 2022 Jul 29.

Division of Ophthalmology, Department of Surgery, McMaster University, Waterloo Regional Campus, Kitchener-Waterloo, Ontario, Canada.

Purpose: Studies comparing the two different formulations of bimatoprost, 0.03% and 0.01%, have shown similar efficacy, but a better adverse effect profile for bimatoprost 0.01% in patients with primary open-angle glaucoma (POAG) and ocular hypertension (OHT). This study assesses the efficacy and tolerability of switching from bimatoprost 0.01% to 0.03% in a patient population with broader spectrum of diagnoses in a real-world clinical setting.

Design: Single-centre retrospective observational switch study.

Methods: Selected patients were on initial topical therapy with bimatoprost 0.01% prior to switching to bimatoprost 0.03%. Intraocular pressure (IOP) was collected from their pre-switch visit, 6- and 12-week after switch. Paired two-sample -test was performed to compare IOP at different time points versus baseline. Worsening of hyperemia and other adverse events after the switch were identified. Subgroup analysis was performed for POAG and OHT, secondary open-angle glaucoma (SOAG, including pseudoexfoliative and pigmentary glaucoma), normal tension glaucoma (NTG), and angle closure glaucoma (ACG).

Results: The study population consisted of 248 eyes (143 patients). There was a significant mean IOP reduction of 1.0 ± 3.7 mmHg (p < 0.001, n = 248) from baseline to week-6 and 1.6 ± 4.0 mmHg (p < 0.001, n = 142) from baseline to week-12 after switch. The IOP reduction was statistically significant in patients with POAG and OHT (6-week: 1.0 ± 3.8 mmHg, n = 76; 12-week: 1.5 ± 4.1 mmHg, n = 49), ACG (6-week: 1.5 ± 4.1 mmHg, n = 72; 12-week: 2.3 ± 4.5 mmHg, n = 46), and NTG (6-week: 0.83 ± 2.5 mmHg, n = 42; 12-week: 1.12 ± 2.1 mmHg, n = 25). Patients with SOAG did not show statistically significant reduction in IOP at 6- or 12-week after switch. Forty-two (29%) of 143 patients experienced adverse events, with the most common being hyperemia (16%).

Conclusion: Significant reduction in IOP could be seen after switching from bimatoprost 0.01% to bimatoprost 0.03% in various types of glaucoma except SOAG. Intolerance after switch may be experienced, though not in the majority of cases.
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http://dx.doi.org/10.2147/OPTH.S368214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9346412PMC
July 2022

Nitidine Chloride Alleviates Inflammation and Cellular Senescence in Murine Osteoarthritis Through Scavenging ROS.

Front Pharmacol 2022 22;13:919940. Epub 2022 Jul 22.

Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Osteoarthritis (OA) is one of the most common chronic musculoskeletal disorder worldwide, representing a major source of disability, pain and socioeconomic burden. Yet the effective pharmaceutical treatments applied in the clinical works are merely symptomatic management with uncertainty around their long-term safety and efficacy, namely no drugs currently are capable of modulating the biological progression of OA. Here, we identified the potent anti-inflammatory as well as anti-oxidative properties of Nitidine Chloride (NitC), a bioactive phytochemical alkaloid extracted from natural herbs, in IL-1β-treated rat articular chondrocytes (RACs), LPS-stimulated RAW 264.7 and rat osteoarthritic models . We demonstrated NitC remarkably inhibited the production of inflammatory mediators including COX2 and iNOS, suppressed the activation of MAPK and NF-κB cell signaling pathway and reduced the expression of extracellular matrix (ECM) degrading enzymes including MMP3, MMP9 and MMP13 in IL-1β-treated RACs. Several emerging bioinformatics tools were performed to predict the underlying mechanism, the result of which indicated the potential reactive oxygen species (ROS) clearance potential of NitC. Further, NitC exhibited its anti-oxidative potential through ameliorating cellular senescence in IL-1β-treated RACs and decreasing NLRP3 inflammasomes activation in LPS-stimulated RAW 264.7 scavenging ROS. Additionally, X-ray, micro-CT and other experiments demonstrated that intra-articular injection of NitC significantly alleviated the cartilage erosion, ECM degradation and subchondral alterations in OA progression. In conclusion, the present study reported the potent anti-inflammatory and anti-oxidative potential of NitC in OA biological process, providing a promising therapeutic agent for OA management.
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http://dx.doi.org/10.3389/fphar.2022.919940DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353946PMC
July 2022

Blue Revolution for Food Security under Carbon Neutrality: A Case from the Water-saving and Drought-resistance Rice.

Mol Plant 2022 Jul 31. Epub 2022 Jul 31.

Shanghai Collaborative Innovation Center of Agri-Seeds, Shanghai Agrobiological Gene Center, Shanghai, China; Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai, China. Electronic address:

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http://dx.doi.org/10.1016/j.molp.2022.07.014DOI Listing
July 2022

The characteristics of glucose metabolism and functional connectivity in posterior default network during nondemented aging: relationship with executive function performance.

Cereb Cortex 2022 Jul 31. Epub 2022 Jul 31.

School of Artificial Intelligence, Beijing Normal University, Beijing 100875, P.R. China.

Background: Understanding the characteristics of intrinsic connectivity networks (ICNs) in terms of both glucose metabolism and functional connectivity (FC) is important for revealing cognitive aging and neurodegeneration, but the relationships between these two aspects during aging has not been well established in older adults.

Objective: This study is to assess the relationship between age-related glucose metabolism and FC in key ICNs, and their direct or indirect effects on cognitive deficits in older adults.

Methods: We estimated the individual-level standard uptake value ratio (SUVr) and FC of eleven ICNs in 59 cognitively unimpaired older adults, then analyzed the associations of SUVr and FC of each ICN and their relationships with cognitive performance.

Results: The results showed both the SUVr and FC in the posterior default mode network (pDMN) had a significant decline with age, and the association between them was also significant. Moreover, both decline of metabolism and FC in the pDMN were significantly correlated with executive function decline. Finally, mediation analysis revealed the glucose metabolism mediated the FC decline with age and FC mediated the executive function deficits.

Conclusions: Our findings indicated that covariance between glucose metabolism and FC in the pDMN is one of the main routes that contributes to age-related executive function decline.
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http://dx.doi.org/10.1093/cercor/bhac248DOI Listing
July 2022

A two-stage process for the autotrophic and mixotrophic conversion of C1 gases into bacterial cellulose.

Bioresour Technol 2022 Jul 27;361:127711. Epub 2022 Jul 27.

School of Chemistry, Chemical Engineering, and Life Science, Wuhan University of Technology, Wuhan 430070, PR China; Shaoxing Institute for Advanced Research, Wuhan University of Technology, Shaoxing 312300, PR China.

Gas fermentation is a well-established process for the conversion of greenhouse gases from industrial wastes into valuable multi-carbon chemicals. Here, a two-stage process was developed to expand the product range of gas fermentation and synthesized the versatile biopolymer bacterial cellulose (BC). In the first stage, the acetogen Clostridium autoethanogenum was cultivated with H:CO:CO and produced ethanol and acetate. In the second stage, BC-synthesizing Komagataeibacter sucrofermentans was grown in the spent medium from gas fermentation. K. sucrofermentans was able to produce BC autotrophically from gas-derived metabolites alone as well as mixotrophically with the addition of exogenous glucose. In these circumstances, 1.31 g/L BC was synthesized with a major energetic contribution from C1 gas fermentation products. Mixotrophic BC characterization reveals unique properties including augmented mechanical strength, porosity, and crystallinity. This proof-of-concept process demonstrates that BC can be produced from gases and holds good potential for the efficient conversion of C1 wastes.
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http://dx.doi.org/10.1016/j.biortech.2022.127711DOI Listing
July 2022

Economic Value of Fosaprepitant-Containing Regimen in the Prevention of Chemotherapy-Induced Nausea and Vomiting in China: Cost-Effectiveness and Budget Impact Analysis.

Front Public Health 2022 12;10:913129. Epub 2022 Jul 12.

Department of Regulatory Science and Pharmacoeconomics, School of Pharmacy, Nanjing Medical University, Nanjing, China.

Objective: The purpose of this study was to evaluate the cost-effectiveness and budget impact of fosaprepitant (FosAPR)-containing regimen for the prevention of chemotherapy-induced nausea and vomiting (CINV) among patients receiving high emetogenic chemotherapy (HEC) from the Chinese payer's perspective.

Methods: A decision tree model was established to measure the 5-day costs and health outcomes between the APR-containing regimen (aprepitant, granisetron, and dexamethasone) and FosAPR-containing regimen (fosaprepitant, granisetron, and dexamethasone). Clinical data were derived from a randomized, double-blind controlled trial on Chinese inpatients who received HEC. Quality-adjusted life-years (QALYs) were used to estimate the utility outcomes and the incremental cost-effectiveness ratio (ICER) was calculated to assess the economics of FosAPR. A static budget impact model was developed to assess the impact of FosAPR as a new addition to the National Reimbursement Drug List (NRDL) on the medical insurance fund within 3 years in Nanjing, China.

Results: Compared with APR, FosAPR had a mean health-care savings of ¥121.56 but got a reduction of 0.0001815 QALY, resulting in an ICER of ¥669926.19 per QALY. Deterministic sensitivity analysis revealed that the cost of APR was the most influential factor to the ICER. The cost of FosAPR and the complete control rate of the delayed period also had a high impact on the results. According to the probabilistic analysis, the acceptability of FosAPR was more than 80% when the Chinese willingness-to-pay (WTP) was ¥215,999. FosAPR would lead to a 3-year medical insurance payment increase of ¥1.84 million compared with ¥1.49 million before FosAPR entered NRDL in Nanjing. The total budget increased with a cumulative cost of ¥694,829 and covered an additional 341 patients who benefited from FosAPR in Nanjing. Deterministic sensitivity analysis showed that the model of budget impact analysis was stable.

Conclusion: FosAPR had a similar treatment effect to APR but was cost-effective in China at the current WTP threshold. The total budget of medical insurance payments of Nanjing slightly increased year by year after the inclusion of FosAPR. Its inclusion in the NRDL would be acceptable and also expand the coverage of patients who benefited from FosAPR.
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http://dx.doi.org/10.3389/fpubh.2022.913129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9315060PMC
August 2022

Study on Vertical Joint Performance of Single-Faced Superposed Shear Wall Based on Finite Element Analysis and Calculation.

Comput Intell Neurosci 2022 18;2022:2418874. Epub 2022 Jul 18.

School of Civil Engineering, Anhui Jianzhu University, Hefei 230601, China.

In this paper, a new type of single-faced superposed shear wall system is proposed based on the single-faced superposed shear wall structure. The nonlinear finite element analysis of a new splicing form of single-faced superposed shear wall components is carried out by using the method of combining experimental research and ABAQUS finite element software analysis. The impact of noncolumn on the connection performance of vertical seam is studied. The analysis shows no significant difference in the effect of eliminating concealed column on the hysteretic performance and bearing capacity of the single-faced superposed shear wall with the joint. The single-faced composite shear wall with two different splicing forms presents bending-shear failure. The single-faced composite shear wall model without concealed column design can also maintain good overall performance and seismic behavior.
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http://dx.doi.org/10.1155/2022/2418874DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313991PMC
July 2022

Highly Aligned Bacterial Nanocellulose Films Obtained During Static Biosynthesis in a Reproducible and Straightforward Approach.

Adv Sci (Weinh) 2022 Jul 21:e2201947. Epub 2022 Jul 21.

Institut Ciencia de Materials de Barcelona (ICMAB-CSIC), Campus UAB, Bellaterra, 08193, Spain.

Bacterial nanocellulose (BNC) is usually produced as randomly-organized highly pure cellulose nanofibers films. Its high water-holding capacity, porosity, mechanical strength, and biocompatibility make it unique. Ordered structures are found in nature and the properties appearing upon aligning polymers fibers inspire everyone to achieve highly aligned BNC (A-BNC) films. This work takes advantage of natural bacteria biosynthesis in a reproducible and straightforward approach. Bacteria confined and statically incubated biosynthesized BNC nanofibers in a single direction without entanglement. The obtained film is highly oriented within the total volume confirmed by polarization-resolved second-harmonic generation signal and Small Angle X-ray Scattering. The biosynthesis approach is improved by reusing the bacterial substrates to obtain A-BNC reproducibly and repeatedly. The suitability of A-BNC as cell carriers is confirmed by adhering to and growing fibroblasts in the substrate. Finally, the thermal conductivity is evaluated by two independent approaches, i.e., using the well-known 3ω-method and a recently developed contactless thermoreflectance approach, confirming a thermal conductivity of 1.63 W mK in the direction of the aligned fibers versus 0.3 W mK perpendicularly. The fivefold increase in thermal conductivity of BNC in the alignment direction forecasts the potential of BNC-based devices outperforming some other natural polymer and synthetic materials.
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http://dx.doi.org/10.1002/advs.202201947DOI Listing
July 2022

Intestinal FFA3 Mediates Obesogenic Effects in Mice on a Western Diet.

Am J Physiol Endocrinol Metab 2022 Jul 20. Epub 2022 Jul 20.

Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Illinois at Chicago, Chicago, IL, United States.

Free fatty acid receptor 3 (FFA3) is a recently-deorphanized G protein-coupled receptor. Its ligands are short-chain fatty acids (SCFAs), which are key nutrients derived from the gut microbiome fermentation process that play diverse roles in the regulation of metabolic homeostasis and glycemic control. FFA3 is highly expressed within the intestine, where its role and its effects on physiology and metabolism are unclear. Previous in vivo studies of this receptor have relied on global knockout mouse models, making it difficult to isolate intestine-specific roles of FFA3. To overcome this challenge, we generated an intestine-specific knockout mouse model for FFA3, Villin-Cre-FFA3 (Vil-FFA3). Model validation and general metabolic assessment of male mice on standard chow revealed no major congenital defects. Because dietary changes are known to alter gut microbial composition, and thereby SCFA production, an obesogenic challenge was performed on male Vil-FFA3 mice and their littermate controls to probe for a phenotype on a high-fat, high-sugar "Western diet" (WD) compared to a low-fat control diet (CD). Vil-FFA3 mice versus FFA3 controls on WD, but not CD, were protected from the development of diet-induced obesity and exhibited significantly less fat mass as well as smaller adipose depositions and adipocytes. Glycemic control was modestly improved in the WD-fed FFA3 group as indicated by lower fasting glucose levels. Intestinal inflammation was also significantly reduced in the WD-fed Vil-FFA3 mice, further supporting protection from obesogenic effects. Furthermore, we observed lower levels of gastric inhibitory protein (GIP) in the WD-fed Vil-FFA3 mice, which may contribute to phenotypic changes. Our findings suggest a novel role of intestinal FFA3 in promoting the metabolic consequences of a WD, including the development of obesity and inflammation, and loss of glycemic control. Moreover, these data support an intestine-specific role of FFA3 in whole-body metabolic homeostasis and in the development of adiposity and hyperglycemia.
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http://dx.doi.org/10.1152/ajpendo.00016.2022DOI Listing
July 2022

Evaluation of the Glymphatic System Using the DTI-ALPS Index in Patients with Spontaneous Intracerebral Haemorrhage.

Oxid Med Cell Longev 2022 5;2022:2694316. Epub 2022 Jul 5.

Department of Radiology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, 221000 Jiangsu, China.

Objective: To investigate the function of the human glymphatic system (GS) in patients with spontaneous intracerebral haemorrhage (sICH) using diffusion tensor imaging analysis along with the perivascular space (DTI-ALPS).

Methods: Twenty patients with sICH and 31 healthy controls (HCs) were recruited for DTI and susceptibility-weighted imaging scanning. The diffusivity along the perivascular spaces, as well as the projection fibres and association fibres, was evaluated separately. The DTI-ALPS index of each subject was also calculated. Two-sample -tests and paired -tests were performed to analyse the difference in ALPS scores between patients and HCs, as well as that between the lesion side and contralateral side. Pearson correlation analysis was used to observe the relationship between disease duration and GS function.

Results: The DTI-ALPS index on the lesion side was significantly lower than that of the contralateral side in patients with sICH ( < 0.01, = -5.77), and it was also significantly lower than that of the ipsilateral side of HCs ( < 0.01, = -9.50). No significant differences were found in the DTI-ALPS index on the nonlesion side between patients and HCs ( = 0.96, = 0.05) or between the left and right cerebral hemispheres of HCs ( = 0.41, = -0.83). The DTI-ALPS index of the lesion side in patients with sICH was significantly correlated with disease duration ( = 0.018, = 0.537).

Conclusions: The present study confirmed that GS dysfunction on the ipsilateral side of the lesion is impaired in patients with haemorrhagic stroke, indicating that the GS may be a separate system in the left and right cerebral hemispheres. The DTI-ALPS index can reflect disease duration. These findings have significant implications for understanding sICH from a new perspective.
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http://dx.doi.org/10.1155/2022/2694316DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9277160PMC
July 2022

Development and application of a novel β-diketone difluoroboron-derivatized fluorescent probe for sensitively detecting HS.

Spectrochim Acta A Mol Biomol Spectrosc 2022 Jul 9;281:121609. Epub 2022 Jul 9.

Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China. Electronic address:

Hydrogen sulfide{Wang, 2018 #4}{Wang, 2018 #4}{Zhong, 2020 #9} (HS) is a poisonous and harmful gas molecule. Certain concentrations of HS{Liu, 2021 #8} can irritate the eyes, respiratory system, and central nervous system of human beings. Therefore, it was an urgent need for highly selective, anti-interference, and sensitive detection technology for hydrogen sulfide. Herein, a novel "turn-on" fluorescent probe 1-(2-(6,6-dimethylbicyclo[3.1.1]heptyl-2-ene-2-yl))-9-(4-(dimethylaminophenyl))non-1,6,8-triene-3,5-dione boron difluoride complex (MCBF) was designed and synthesized for detecting HS sensitively. MCBF displayed a remarkable fluorescence enhancement response to HS with a large Stokes shift of 220 nm. The sensitive detection of MCBF towards HS owned good selectivity, fast response time (6 min), excellent photostability, and low detection limit (0.44 μM). The sensing mechanism of MCBF towards HS was well confirmed by HRMS analysis, H NMR titration, and density functional theory (DFT) calculations. What's more, probe MCBF was successfully applied to detect the contained HS in red wine, which showed the potential practicability of MCBF in real samples analysis.
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http://dx.doi.org/10.1016/j.saa.2022.121609DOI Listing
July 2022

Mechanism of Negative Emotions of the Elderly in Normalization Period of COVID-19: A Mediated Mediation Model.

Authors:
Kai Xu

Front Public Health 2022 28;10:941958. Epub 2022 Jun 28.

School of Educational Science, Luoyang Normal University, Luoyang, China.

To explore the mechanism of negative emotions of the elderly in normalization period of COVID-19. The self-rating Depression Anxiety Stress Scale, epidemic attention scale, subjective economic status scale and physical health perception scale were used to investigate 318 elderly people in 2021. There were significant differences in negative emotions among the elderly in Henan in China with different gender, education background, medical insurance and whether they contacted suspected cases (all < 0.05), but there was no significant difference on religious belief ( > 0.05); Attention to epidemic information was positively correlated with negative emotion ( = 0.492, < 0.01), and negatively correlated with subjective economic status ( = -0.138, < 0.05); Negative emotions were negatively correlated with subjective economic status ( = -0.455, < 0.01) and health perception ( = -0.277, < 0.01); health perception was no significant correlation with epidemic attention( = -0.047, > 0.05) and subjective economic status ( = -0.033, > 0.05). Bootstrap test found that epidemic attention can significantly predict negative emotion of the elderly (β = 0.492, < 0.001), subjective economic status played a partial mediating role between epidemic attention and negative emotions (β = 0.438, -0.395, < 0.001), and health perception moderated the first half of the mediating path (β = 0.403, < 0.001, 95% CI = [0.286~0.521]). Epidemic attention has a significant positive impact on the negative emotions of the elderly in Henan during normalization period of COVID-19, and it has effect indirectly through subjective economic status; health perception plays a moderator role in the impact of epidemic attention on subjective economic status.
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http://dx.doi.org/10.3389/fpubh.2022.941958DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9275750PMC
July 2022

Design and Fabrication of Extremely Lightweight Truss-Structured Metal Mirrors.

Materials (Basel) 2022 Jun 29;15(13). Epub 2022 Jun 29.

Key Laboratory of Optical System Advanced Manufacturing Technology, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China.

Three-dimensional printing, also called additive manufacturing (AM), offers a new vision for optical components in terms of weight reduction and strength improvement. A truss, which is a triangulated system of members that are structured and connected in such a way that they mainly bear axial force, is commonly used in steel structures to improve stiffness and reduce weight. Combining these two technologies, an extremely lightweight truss-structured mirror was proposed. First, the finite element analyses (FEA) on surface shape deviation and modal properties were carried out. Results showed that the mirrors had sufficient stiffness and a high weight reduction of up to 85%. In order to verify their performance, the truss-structured mirror blanks were fabricated with AM technology. After that, both the preprocessing and the postprocessing of the mirrors were carried out. The results show that without NiP coating, a surface shape deviation of 0.353λ (PV) and 0.028 λ (RMS) (λ = 632.8 nm) with a roughness of Ra 2.8 nm, could be achieved. Therefore, the truss-structured mirrors in this study have the characteristics of being extremely lightweight and having improved stiffness as well as strong temperature stability.
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http://dx.doi.org/10.3390/ma15134562DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9267368PMC
June 2022

3-Year Outcomes After 2-Stent With Provisional Stenting for Complex Bifurcation Lesions Defined by DEFINITION Criteria.

JACC Cardiovasc Interv 2022 07;15(13):1310-1320

Division of Cardiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China; College of Pharmacy, Nanjing Medical University, Nanjing, China. Electronic address:

Background: The multicenter and randomized DEFINITION II (Two-Stent vs Provisional Stenting Techniques for Patients With Complex Coronary Bifurcation Lesions) trial showed less 1-year target lesion failure (TLF) after a 2-stent approach for complex coronary bifurcation lesions compared with provisional stenting (PS). The authors report the 3-year clinical outcome of the DEFINITION II trial.

Objectives: The aim of the present study was to investigate the difference in TLF at 3 years after a planned 2-stent approach vs PS for complex coronary bifurcation lesions stratified by DEFINITION (Definitions and Impact of Complex Bifurcation Lesions on Clinical Outcomes After Percutaneous Coronary Intervention Using Drug-Eluting Stents) criteria.

Methods: A total of 653 patients with complex coronary bifurcation lesions were randomly assigned to either the 2-stent group or the PS group in the DEFINITION II trial and were followed for 3 years. The primary endpoint was the occurrence of TLF at 3 years. Stent thrombosis was the safety endpoint.

Results: At 3 years, TLF had occurred in 52 patients (16.0%) in the PS group and in 34 (10.4%) patients in the 2-stent group (HR: 0.63; 95% CI: 0.41-0.97; P = 0.035), driven mainly by increased target vessel myocardial infarction (8.0% vs 3.7%; HR: 0.45; 95% CI: 0.23-0.89; P = 0.022) and target lesion revascularization (8.3% vs 4.3%; HR: 0.50; 95% CI: 0.26-0.96; P = 0.038). There was no difference in TLF between the 2 groups between year 1 and year 3.

Conclusions: For patients with complex coronary bifurcations who reach 1-year postprocedure without experiencing endpoint events, there is still a risk for future events. The type of procedure performed initially is no longer a future event risk determinant. (Two-Stent vs Provisional Stenting Techniques for Patients With Complex Coronary Bifurcation Lesions; NCT02284750).
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http://dx.doi.org/10.1016/j.jcin.2022.05.026DOI Listing
July 2022

Effect of the transanal drainage tube on preventing anastomotic leakage after laparoscopic surgery for rectal cancer: a systematic review and meta-analysis.

Int J Colorectal Dis 2022 Jul 5. Epub 2022 Jul 5.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Gastrointestinal Surgery IV, Peking University Cancer Hospital & Institute, Haidian District, No. 52 Fucheng Road, Beijing, 100142, China.

Purpose: Anastomotic leakage (AL) is a common postoperative complication of rectal cancer, and transanal drainage tube (TDT) efficacy is still contentious. This study aimed to evaluate the TDT effect on AL prevention.

Methods: All relevant papers were searched by using a predefined search strategy (two randomized controlled trials (RCTs), one prospective study, and four retrospective studies). Meta-analysis was conducted to estimate AL and re-operation pooled rates.

Results: A total of 7 studies (1556 patients) were included: No significant statistic difference was found between two groups on AL rate (odds ratio (OR) 0.61, P = 0.11) and re-operation rate (OR 0.52, P = 0.10). For subgroup analysis, significant statistic difference was found between two groups on AL rate (OR 0.29, P = 0.002) and re-operation rate (OR 0.15, P = 0.04) in patients without neoadjuvant therapy. As for patients without diverting stoma, the AL rate (OR 0.35, P = 0.002) was significantly lower than that in patients without TDT.

Conclusions: TDT may reduce AL morbidity and re-operation rate for patients without high risk of AL, but may be useless for those in high-risk situations.
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http://dx.doi.org/10.1007/s00384-022-04201-yDOI Listing
July 2022

Histone Methylation Is Required for Virulence, Conidiation, and Multi-Stress Resistance of .

Front Microbiol 2022 16;13:924476. Epub 2022 Jun 16.

Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou, China.

Histone methylation, which is critical for transcriptional regulation and various biological processes in eukaryotes, is a reversible dynamic process regulated by histone methyltransferases (HMTs) and histone demethylases (HDMs). This study determined the function of 5 HMTs (, , , , and ) and 1 HDMs () in the phytopathogenic fungus by analyzing targeted gene deletion mutants. The vegetative growth, conidiation, and pathogenicity of ∆ and ∆ were severely inhibited indicating that and play critical roles in cell development in . Multiple stresses analysis revealed that both and were involved in the adaptation to cell wall interference agents and osmotic stress. Meanwhile, ∆ and ∆ displayed serious vegetative growth defects in sole carbon source medium, indicating that and play an important role in carbon source utilization. In addition, ∆ colony displayed white in color, while the wild-type colony was dark brown, indicating is an essential gene for melanin biosynthesis in . was required for the resistance to oxidative stress. On the other hand, all of ∆, ∆, and ∆ mutants displayed wild-type phenotype in vegetative growth, multi-stress resistance, pathogenicity, carbon source utilization, and melanin biosynthesis. To explore the regulatory mechanism of and , RNA-seq of these mutants and wild-type strain was performed. Phenotypes mentioned above correlated well with the differentially expressed genes in ∆ and ∆ according to the KEGG and GO enrichment results. Overall, our study provides genetic evidence that defines the central role of HMTs and HDMs in the pathological and biological functions of .
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http://dx.doi.org/10.3389/fmicb.2022.924476DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245015PMC
June 2022

Electrically driven single microwire-based single-mode microlaser.

Light Sci Appl 2022 Jun 29;11(1):198. Epub 2022 Jun 29.

College of Physics, MIIT Key Laboratory of Aerospace Information Materials and Physics, Key Laboratory for Intelligent Nano Materials and Devices, Nanjing University of Aeronautics and Astronautics, No. 29 Jiangjun Road, Nanjing, 211106, China.

Engineering the lasing-mode oscillations effectively within a laser cavity is a relatively updated attentive study and perplexing issue in the field of laser physics and applications. Herein, we report a realization of electrically driven single-mode microlaser, which is composed of gallium incorporated zinc oxide microwire (ZnO:Ga MW) with platinum nanoparticles (PtNPs, d ~ 130 nm) covering, a magnesium oxide (MgO) nanofilm, a Pt nanofilm, and a p-type GaN substrate. The laser cavity modes could resonate following the whispering-gallery mode (WGM) among the six side surfaces by total internal reflection, and the single-mode lasing wavelength is centered at 390.5 nm with a linewidth of about 0.18 nm. The cavity quality factor Q is evaluated to about 2169. In the laser structure, the usage of Pt and MgO buffer layers can be utilized to engineer the band alignment of ZnO:Ga/GaN heterojunction, optimize the p-n junction quality and increase the current injection. Thus, the well-designed device structure can seamlessly unite the electron-hole recombination region, the gain medium, and optical microresonator into the [email protected]:Ga wire perfectly. Such a single MW microlaser is essentially single-mode regardless of the gain spectral bandwidth. To study the single-mode operation, PtNPs working as superabsorber can engineering the multimode lasing actions of ZnO:Ga MWs even if their dimensions are typically much larger than that of lasing wavelength. Our findings can provide a straightforward and effective scheme to develop single-mode microlaser devices based on one-dimensional wire semiconductors.
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http://dx.doi.org/10.1038/s41377-022-00874-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240044PMC
June 2022

Learning to Detect 3D Symmetry From Single-View RGB-D Images With Weak Supervision.

IEEE Trans Pattern Anal Mach Intell 2022 Jun 28;PP. Epub 2022 Jun 28.

3D symmetry detection is a fundamental problem in computer vision and graphics. Most prior works detect symmetry when the object model is fully known, few studies symmetry detection on objects with partial observation, such as single RGB-D images. Recent work addresses the problem of detecting symmetries from incomplete data with a deep neural network by leveraging the dense and accurate symmetry annotations. However, due to the tedious labeling process, full symmetry annotations are not always practically available. In this work, we present a 3D symmetry detection approach to detect symmetry from single-view RGB-D images without using symmetry supervision. The key idea is to train the network in a weakly-supervised learning manner to complete the shape based on the predicted symmetry such that the completed shape be similar to existing plausible shapes. To achieve this, we first propose a discriminative variational autoencoder to learn the shape prior in order to determine whether a 3D shape is plausible or not. Based on the learned shape prior, a symmetry detection network is present to predict symmetries that produce shapes with high shape plausibility when completed based on those symmetries. Moreover, to facilitate end-to-end network training and multiple symmetry detection, we introduce a new symmetry parametrization for the learning-based symmetry estimation of both reflectional and rotational symmetry. The proposed approach, coupled symmetry detection with shape completion, essentially learns the symmetry-aware shape prior, facilitating more accurate and robust symmetry detection. Experiments demonstrate that the proposed method is capable of detecting reflectional and rotational symmetries accurately, and shows good generality in challenging scenarios, such as objects with heavy occlusion and scanning noise. Moreover, it achieves state-of-the-art performance, improving the F1-score over the existing supervised learning method by 2%-11% on the ShapeNet and ScanNet datasets.
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http://dx.doi.org/10.1109/TPAMI.2022.3186876DOI Listing
June 2022

H3K27M Mutation Doesn't Mean Worse Prognosis in Old Patients.

Front Oncol 2022 8;12:912166. Epub 2022 Jun 8.

Huashan Hospital, Fudan University, Shanghai, China.

Objective: Diffuse midline glioma (DMG), H3K27 altered is a new entity that has become widely recognized. However, studies concerning DMG in adult patients remain rare. We did a retrospective study covering the largest amount of patients to date to analyze the clinicopathological characteristics of diffuse glioma in midline structures of the adult.

Methods: We reviewed 108 cases of adult DMG, collected their clinical data, and pathological results including H3K27 mutation. Summarized their features and the connection with overall survival in different age groups.

Results: Among 108 cases, 79 tumors were located at the thalamus. 38 patients had H3K27M mutation, whose average age was 35.7 years. The median overall survival of H3K27M-mutant gliomas and the 80 H3K27M wild-type gliomas were both 12 months. For young patients (age ≤ 35), The median survival time of the H3K27M-mutant was 18 months, while that of the H3K27M wild-type was 37 months. For older patients (age>35), the median survival time of the H3K27M-mutant was 16 months, while that of the H3K27M wild-type was 13 months. Other clinicopathological factors including sex, tumor location, the approach of surgery, histological grade, ATRX, and P53 were statistically irrelevant to prognosis.

Conclusion: The DMG in adults mainly occurred in the thalamus. H3K27M mutations tend to happen more frequently in young adults, and this genetic alteration results in a worse outcome only in young patients (≤35). For old patients, age is the only independent prognostic factor. Patients who underwent different surgical operations including biopsy, subtotal resection, and total resection had similar prognoses.
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http://dx.doi.org/10.3389/fonc.2022.912166DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214035PMC
June 2022

Glycosylation and its research progress in endometrial cancer.

Clin Transl Oncol 2022 Jun 25. Epub 2022 Jun 25.

Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Endometrial cancer (EC) is one of the most common tumors in the female reproductive system, which seriously threatens women's health, particularly in developed countries. 13% of the patients with EC have a poor prognosis due to recurrence and metastasis. Therefore, identifying good predictive biomarkers and therapeutic targets is critical to enable the early detection of metastasis and improve the prognosis. For decades, extensive studies had focused on glycans and glycoproteins in the progression of cancer. The types of glycans that are covalently attached to the polypeptide backbone, usually via nitrogen or oxygen linkages, are known as N‑glycans or O‑glycans, respectively. The degree of protein glycosylation and the aberrant changes in the carbohydrate structures have been implicated in the extent of tumorigenesis and reported to play a critical role in regulating tumor invasion, metabolism, and immunity. This review summarizes the essential biological role of glycosylation in EC, with a focus on the recent advances in glycomics and glycosylation markers, highlighting their implications in the diagnosis and treatment of EC.
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http://dx.doi.org/10.1007/s12094-022-02858-zDOI Listing
June 2022

Non-Targeted Metabolomics Analysis Revealed the Characteristic Non-Volatile and Volatile Metabolites in the Wuyi Rock Tea () from Different Culturing Regions.

Foods 2022 Jun 9;11(12). Epub 2022 Jun 9.

College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Rougui Wuyi Rock tea (WRT) with special flavor can be affected by multiple factors that are closely related to the culturing regions of tea plants. The present research adopted non-targeted metabolomics based on liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS), aroma activity value method (OAV), and chemometrics to analyze the characteristic metabolites of three Rougui WRTs from different culturing regions. The results of sensory evaluation showed that the three Rougui Wuyi Rock teas had significantly different flavor qualities, especially in taste and aroma. Rougui (RG) had a heavy and mellow taste, while cinnamon-like odor Rougui (GPRG) and floral and fruity odor Rougui (HGRG) had a thick, sweet, and fresh taste. The cinnamon-like odor was more obvious and persistent in GPRG than in RG and HGRG. HGRG had floral and fruity characteristics such as clean and lasting, gentle, and heavy, which was more obvious than in RG and GPRG. The results of principal component analysis (PCA) showed that there were significant metabolic differences among the three Rougui WRTs. According to the projection value of variable importance (VIP) of the partial least squares discriminant analysis (PLS-DA), 24 differential non-volatile metabolites were identified. The PLSR analysis results showed that rutin, silibinin, arginine, lysine, dihydrocapsaicin, etc. may be the characteristic non-volatiles that form the different taste outlines of Rougui WRT. A total of 90 volatiles, including aldehydes, alcohols, esters, and hydrocarbons, were identified from the three flavors of Rougui WRT by using GC-MS. Based on OAV values and PLS-DA analysis, a total of 16 characteristic volatiles were identified. The PLSR analysis results showed that 1-penten-3-ol, α-pinene, 2-carene, β-Pinene, dehydrolinalool, adipaldehyde, D-limonene, saffron aldehyde, and 6-methyl-5-hepten-2-one may be the characteristic volatiles that form the different aroma profile of Rougui WRT. These results provide the theoretical basis for understanding the characteristic metabolites that contribute to the distinctive flavors of Rougui WRT.
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http://dx.doi.org/10.3390/foods11121694DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222269PMC
June 2022

The Reduction in Microtubule Arrays Caused by the Dysplasia of the Non-Centrosomal Microtubule-Organizing Center Leads to a Malformed Organ of Corti in the Cx26-Null Mouse.

Biomedicines 2022 Jun 9;10(6). Epub 2022 Jun 9.

Institute of Otorhinolaryngology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Mutations in the gene account for approximately 20-50% of all non-syndromic hereditary deafness cases. The malformed organ of Corti (OC) was observed in different Cx26-null mouse models, which was mainly caused by the developmental arrest of pillar cells (PCs). However, the mechanism of developmental abnormalities in PCs caused by Cx26 deletion is still unclear. In this study, the ultrastructure of PCs at different postnatal days was observed in Cx26-null mice. Knockout of cochlear Cx26 led to the malformed assembly of non-centrosomal microtubule-organizing centers (MTOCs) far from the centrosome rather than near the centrosome. Additionally, the microtubule (MT) arrays emitted by abnormal non-centrosomal MTOCs were significantly reduced. In addition, we found that the protein expression of calmodulin-regulated, spectrin-associated protein2 (camsap2), a microtubule minus-end targeting protein associated with the organization of non-centrosomal MTs, was decreased in juvenile PCs in the Cx26-null group. Our results indicated that the malformation of non-centrosomal MTOCs in cochlear PCs might lead to the corresponding MTs' failure to be captured and anchored in Cx26-null mice, which results in the deformity of OC. Additionally, this abnormal developmental process might be correlated with the reduced expression of camsap2 caused by Cx26 deletion in the early developmental stage.
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http://dx.doi.org/10.3390/biomedicines10061364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219875PMC
June 2022

Biolimus-Coated Balloon in Small-Vessel Coronary Artery Disease: The BIO-RISE CHINA Study.

JACC Cardiovasc Interv 2022 06 25;15(12):1219-1226. Epub 2022 May 25.

General Hospital of Northern Theater Command, Shenyang, People's Republic of China. Electronic address:

Background: Drug-coated balloons are a safe and effective option for patients undergoing percutaneous coronary intervention, but prior randomized studies have exclusively used paclitaxel-coated devices.

Objectives: The aim of this study was to assess for the first time the safety and efficacy of a novel biolimus-coated balloon (BCB) in patients with small-vessel coronary disease.

Methods: In a prospective trial conducted at 10 centers in China, 212 patients with small-vessel native coronary disease (reference vessel diameter 2.0-2.75 mm, lesion length ≤25 mm) were randomized to receive a BCB or an uncoated balloon. The primary endpoint was in-segment late lumen loss at 9 months.

Results: In the per-protocol population, angiographic late lumen loss at 9 months was 0.16 ± 0.29 mm in the BCB group vs 0.30 ± 0.35 mm with the plain balloon (P = 0.001). Late luminal enlargement (positive remodeling) occurred in 29.7% of patients in the BCB group vs 9.8% of patients with plain balloons (P = 0.007). In the full analysis set population, after 12 months, target lesion failure rates were 6.7% in the BCB group vs 13.9% with the plain balloon (HR: 0.47; 95% CI: 0.19-1.16), and rates of the patient-oriented clinical outcome were 14.3% with the BCB vs 21.8% with the plain balloon (HR: 0.64; 95% CI: 0.33-1.24).

Conclusions: In this first-in-human study, a novel BCB showed superior efficacy to plain balloon angioplasty in patients with small-vessel coronary disease undergoing percutaneous coronary intervention. Positive vascular remodeling was more frequent, and there was a trend toward improved clinical outcomes. (A Randomized Trial of a Biolimus-Coated Balloon Versus POBA in Small Vessel Coronary Artery Disease [Brave]; NCT03769623).
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http://dx.doi.org/10.1016/j.jcin.2022.03.024DOI Listing
June 2022

Spatial Distribution and Hierarchical Clustering of β-Amyloid and Glucose Metabolism in Alzheimer's Disease.

Front Aging Neurosci 2022 6;14:788567. Epub 2022 Jun 6.

State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing, China.

Increased amyloid burden and decreased glucose metabolism are important characteristics of Alzheimer's disease (AD), but their spatial distribution and hierarchical clustering organization are still poorly understood. In this study, we explored the distribution and clustering organization of amyloid and glucose metabolism based on F-florbetapir and F-fluorodeoxyglucose PET data from 68 AD patients and 20 cognitively normal individuals. We found that: (i) cortical regions with highest florbetapir binding were the regions with high glucose metabolism; (ii) the percentage changes of amyloid deposition were greatest in the frontal and temporal areas, and the hypometabolism was greatest in the parietal and temporal areas; (iii) brain areas can be divided into three hierarchical clusters by amyloid and into five clusters by metabolism using a hierarchical clustering approach, indicating that adjacent regions are more likely to be grouped into one sub-network; and (iv) there was a significant positive correlation in any pair of amyloid-amyloid and metabolism-metabolism sub-networks, and a significant negative correlation in amyloid-metabolism sub-networks. This may suggest that the influence forms and brain regions of AD on different pathological markers may not be synchronous, but they are closely related.
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http://dx.doi.org/10.3389/fnagi.2022.788567DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207533PMC
June 2022

Clinical application of oral Chinese Patent Medicines in ophthalmology: a scoping review protocol.

BMJ Open 2022 06 20;12(6):e059571. Epub 2022 Jun 20.

Eye Hospital, China Academy of Chinese Medical Sciences, Beijing, China.

Introduction: The prevalence of eye diseases has been increasing worldwide. In China, in addition to conventional medicine, Traditional Chinese Medicine (TCM) plays an important role in maintaining people's vision health. Although less flexible and targeted than TCM decoction, Chinese Patent Medicines (CPMs) are stable and well used. In recent years, CPMs have been increasingly used in ophthalmology clinics by TCM practitioners and by Western doctors in general hospitals. However, comprehensive evidence for using CPMs in ophthalmology is lacking.

Aim: We will apply the methodology of scoping review to systematically search and sort out the available evidence on oral CPMs for the treatment of eye diseases, identify the distribution of evidence in this field and provide a basis for clinical practice and medical decisions.

Methods: The scoping review will be implemented in the following seven steps: (1) defining the research question; (2) searching ), ) and ) for oral CPMs for the treatment of eye diseases; (3) searching Embase, Web of Science, PubMed, Cochrane Library, Chongqing VIP Chinese Scientific Journals Database, Chinese National Knowledge Infrastructure, Chinese Biomedical Literature Database and Wanfang Database for relevant literature published from inception to December 2021; (4) developing eligibility criteria; (5) screening the studies based on inclusion criteria; (6) extracting relevant data and lastly, (7) collating, summarising and reporting the results.

Ethics And Dissemination: Since the scoping review aims at collecting data from publicly available publications, this study does not require ethical approval. The results will be published in a peer-reviewed journal and presented at scientific conferences.
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http://dx.doi.org/10.1136/bmjopen-2021-059571DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9214408PMC
June 2022

Strawberry Vein Banding Virus Movement Protein P1 Interacts With Light-Harvesting Complex II Type 1 Like of to Promote Viral Infection.

Front Microbiol 2022 26;13:884044. Epub 2022 May 26.

Department of Plant Pathology, School of Plant Protection, Anhui Agricultural University, Hefei, China.

Chlorophyll a/b-binding protein of light-harvesting complex II type 1 like (LHC II-1L) is an essential component of photosynthesis, which mainly maintains the stability of the electron transport chain. However, how the LHC II-1L protein of (FvLHC II-1L) affects viral infection remains unclear. In this study, we demonstrated that the movement protein P1 of strawberry vein banding virus (SVBV P1) interacted with FvLHC II-1L and by bimolecular fluorescence complementation and pull-down assays. SVBV P1 was co-localized with FvLHC II-1L at the edge of epidermal cells of leaves, and FvLHC II-1L protein expression was upregulated in SVBV-infected . We also found that FvLHC II-1L effectively promoted SVBV P1 to compensate for the intercellular movement of movement-deficient potato virus X (PVX) and the systemic movement of movement-deficient cucumber mosaic virus (CMV). Transient overexpression of FvLHC II-1L and inoculation of an infectious clone of SVBV showed that the course of SVBV infection in was accelerated. Collectively, the results showed that SVBV P1 protein can interact with FvLHC II-1L protein, which in turn promotes infection by SVBV.
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http://dx.doi.org/10.3389/fmicb.2022.884044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201980PMC
May 2022

GSK-J4, a Specific Histone Lysine Demethylase 6A Inhibitor, Ameliorates Lipotoxicity to Cardiomyocytes Preserving H3K27 Methylation and Reducing Ferroptosis.

Front Cardiovasc Med 2022 2;9:907747. Epub 2022 Jun 2.

Department of Biochemistry and Molecular Biology, School of Basic Medicine and the Collaborative Innovation Center for Brain Science, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Changes in modern lifestyle provoke a series of metabolic stresses such as hyperlipidemia. Excessive free fatty acids induce cardiomyocyte metabolic reprogramming and rearrangement of the lipid content of cardiomyocyte and promote oxidative stress. As a newly defined lipid peroxidation-related cell death pathway, the role of ferroptosis in metabolic stress-induced cardiomyocyte injury is poorly revealed. Our work indicates that GSK-J4, a histone lysine demethylase 6A/6B dual inhibitor, can alleviate palmitic acid (PA)-induced hypersensitivity to ferroptosis by suppressing H3K27 demethylation. Mechanistically, PA stimulation reduces the H3K27me3 level and hence promotes the expression of ACSL4, a key lipid modulator of ferroptosis. GSK-J4 pretreatment significantly preserves the H3K27me3 level and reduces the ACSL4 level. GSK-J4 also reduces reactive oxygen species to alleviate oxidative stress, which further decreases lipid peroxidation. Taken together, our data suggest that cardiomyocyte undergoes epigenetic reprogramming under metabolic challenges, rearranging lipid content, and sensitizing to ferroptosis. GSK-J4 can be a potential drug for treating hyperlipidemia-induced cardiomyocyte injury by targeting epigenetic modulations.
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http://dx.doi.org/10.3389/fcvm.2022.907747DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200982PMC
June 2022

Ultrasensitive Ribo-seq reveals translational landscapes during mammalian oocyte-to-embryo transition and pre-implantation development.

Nat Cell Biol 2022 Jun 13;24(6):968-980. Epub 2022 Jun 13.

Center for Stem Cell Biology and Regenerative Medicine, MOE Key Laboratory of Bioinformatics, School of Life Sciences, Tsinghua University, Beijing, China.

In mammals, translational control plays critical roles during oocyte-to-embryo transition (OET) when transcription ceases. However, the underlying regulatory mechanisms remain challenging to study. Here, using low-input Ribo-seq (Ribo-lite), we investigated translational landscapes during OET using 30-150 mouse oocytes or embryos per stage. Ribo-lite can also accommodate single oocytes. Combining PAIso-seq to interrogate poly(A) tail lengths, we found a global switch of translatome that closely parallels changes of poly(A) tails upon meiotic resumption. Translation activation correlates with polyadenylation and is supported by polyadenylation signal proximal cytoplasmic polyadenylation elements (papCPEs) in 3' untranslated regions. By contrast, translation repression parallels global de-adenylation. The latter includes transcripts containing no CPEs or non-papCPEs, which encode many transcription regulators that are preferentially re-activated before zygotic genome activation. CCR4-NOT, the major de-adenylation complex, and its key adaptor protein BTG4 regulate translation downregulation often independent of RNA decay. BTG4 is not essential for global de-adenylation but is required for selective gene de-adenylation and production of very short-tailed transcripts. In sum, our data reveal intimate interplays among translation, RNA stability and poly(A) tail length regulation underlying mammalian OET.
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http://dx.doi.org/10.1038/s41556-022-00928-6DOI Listing
June 2022

Peripancreatic vascular involvement in patients with type 1 autoimmune pancreatitis.

Hepatobiliary Surg Nutr 2022 Jun;11(3):355-362

Department of Gastroenterology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, China.

Background: Type 1 autoimmune pancreatitis (AIP) is the pancreatic manifestation of IgG4-related disease. However, this benign disease can result in the peripancreatic vascular involvement (PVI) on occasion, which increases the difficulty of diagnosis and treatment of this clinical entity as well as for differentiating it from pancreatic malignancies.

Methods: We retrospectively reviewed the information on demographics, clinical presentation, laboratory, imaging and endoscopic findings of 101 hospitalized patients with type 1 AIP treated in our department. All the patients were divided into non-PVI and PVI groups according to the first hospitalized medical data. Univariate and multivariate analyses were performed to analyse the potential predictive parameter(s) of PVI in AIP patients.

Results: Among the 101 type 1 AIP patients, 52 (51.5%) exhibited PVI, with a male/female ratio 5.5:1. Their average age was 58.37±8.68 years old. Univariate analysis revealed that the location of pancreatitis lesions, including the pancreatic tail (P=0.010), the presence of splenomegaly (P=0.001) and the white blood cell (WBC) number in peripheral blood (P=0.020), were significantly associated with PVI. The location of pancreatitis lesions, including the pancreatic tail (P=0.023), and the presence of splenomegaly (P=0.010) were found to be independent predictors of the development of PVI by a multivariable regression analysis. A total of 18 out of 25 patients in PVI group who underwent corticosteroid treatment and no less than 6 months radiological follow-up showed improvement in vascular lesions, and no case exhibited exacerbation of PVI lesions during follow-up. Of 36 patients in non-PVI group who were followed up for no less than 6 months, only one case exhibited PVI.

Conclusions: This retrospective study demonstrated that type 1 AIP was associated with a high proportion of PVI. Pancreatic tail involvement and splenomegaly may predict the PVI in type 1 AIP. PVI lesions are reversible in a subset of patients.
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http://dx.doi.org/10.21037/hbsn-21-82DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186208PMC
June 2022
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