Publications by authors named "Kai Liu"

2,166 Publications

  • Page 1 of 1

Novel Urea-Based Molecule Functioning as a Solid Electrolyte Interphase Enabler and LiPF Decomposition Inhibitor for Fast-Charging Lithium Metal Batteries.

ACS Appl Mater Interfaces 2022 Aug 18. Epub 2022 Aug 18.

The State Key Laboratory of Chemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.

The practical application of lithium metal batteries is impeded by the growth of dendrites and decomposition of electrolytes especially at high temperature in normal carbonate-based electrolytes. Herein, a novel urea-based molecule, 1,3-dimethyl-2-imidazolidinone (DMI), with a high donor number is proposed, which exhibits an extraordinary solubility of LiNO of over 5 M. As a result, a sufficient amount of LiNO is readily introduced into the carbonate electrolytes with DMI as an additive, and an average coulombic efficiency of 99.1% for lithium plating/stripping is achieved due to a stable solid electrolyte interphase (SEI) rich in inorganic-rich lithium salts. The Li||Li symmetric cell achieves a stable operation for over 2500 h at 0.5 mA cm and 1 mAh cm, and a granular shape of deposited Li metal is still preserved even at a high current density of 10 mA cm. Besides, the decomposition of LiPF is inhibited benefiting from its enhanced dissociation after the addition of DMI/LiNO and DMI's function as a PF scavenger. Consequently, the Li||LiFePO cell succeeds to achieve an excellent capacity retention of 95.6% after 2200 cycles at a high rate of 5C, and a stable operation is realized at a high temperature of 60 °C even under harsh conditions (45 μm ultrathin Li and ∼1.5 mAh cm LiFePO). This work enriches the solvents and additives pool for stable and high-performance lithium metal batteries and will shed light on future developments of advanced battery electrolytes.
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http://dx.doi.org/10.1021/acsami.2c11403DOI Listing
August 2022

Magnetic skyrmions with unconventional helicity polarization in a van der Waals ferromagnet.

Adv Mater 2022 Aug 16:e2204163. Epub 2022 Aug 16.

Physical Science and Engineering Division (PSE), King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.

Skyrmion helicity, which defines the spin swirling direction, is a fundamental parameter that may be utilized to encode data bits in future memory devices. Generally, in centrosymmetric ferromagnets, dipole skyrmions with helicity of -π/2 and π/2 are degenerate in energy, leading to equal populations of both helicities. On the other hand, in chiral materials where the Dzyaloshinskii-Moriya interaction (DMI) is prevailing and the dipolar interaction is negligible, only a preferred helicity is selected by the type of DMI. However, whether there is a rigid boundary between these two regimes remains an open question. Herein, we report the observation of dipole skyrmions with unconventional helicity polarization in a van der Waals ferromagnet Fe GeTe . Combining magnetometry, Lorentz transmission electron microscopy, electrical transport measurements, and micromagnetic simulations, we demonstrate that the short-range superstructures in Fe GeTe results in a localized DMI contribution, which breaks the degeneracy of the opposite helicities and leads to the helicity polarization. Therefore, the helicity feature in Fe GeTe is controlled by both the dipolar interaction and DMI that the former leads to Bloch-type skyrmions with helicity of ±π/2 whereas the latter breaks the helicity degeneracy. This work provides new insights into the skyrmion topology in van der Waals materials. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/adma.202204163DOI Listing
August 2022

Correlation of Ethnicities and Prevalence of Histological Barrett's Esophagus.

Authors:
Kai Liu Yong Zhou

Am J Gastroenterol 2022 May 5. Epub 2022 May 5.

Department of Gastrointestinal Surgery, West China Hospital, Sichuan University, Sichuan Province, China.

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http://dx.doi.org/10.14309/ajg.0000000000001814DOI Listing
May 2022

The Application of the GP Model to Manage Controllable Risk Factors in Stroke Patients with Diabetes Can Effectively Improve the Prognosis and Reduce the Recurrence Rate.

Evid Based Complement Alternat Med 2022 5;2022:5413985. Epub 2022 Aug 5.

Department of General Practice, The First Hospital of Qinhuangdao, Hebei 066000, China.

Objective: The aim of this study is to examine the impacts of general practice model (GP) on prognosis and recurrence of stroke patients with diabetes.

Methods: Ninety patients with stroke combined with diabetes mellitus admitted to our hospital from June 2019 to June 2020 were selected for the study and were randomly and equally divided into 45 cases each in the control and experimental groups for the prospective trial. The patients in the control group received routine treatment while those in the experimental group were treated with GP model. Comparison in treatment effects, patients satisfaction, psychological status, quality of life, glycosylated hemoglobin level, and stroke recurrence was carried out between the two groups.

Results: The experimental group showed markedly better treatment effects ( < 0.05), higher satisfaction degree ( < 0.05), higher HAD ( < 0.05), GQOLI-74 score ( < 0.05), and BI index ( < 0.05), lower level of glycosylated hemoglobin ( < 0.05), and much lower recurrence rate ( < 0.05), as compared to the control group.

Conclusion: The application of the GP model to manage controllable risk factors in stroke patients with diabetes can effectively improve the prognosis and reduce the recurrence rate, which is worthy of clinical application and promotion.
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http://dx.doi.org/10.1155/2022/5413985DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9374552PMC
August 2022

Identification and combinatorial engineering of indole-3-acetic acid synthetic pathways in Paenibacillus polymyxa.

Biotechnol Biofuels Bioprod 2022 Aug 11;15(1):81. Epub 2022 Aug 11.

College of Life Sciences, Shandong Key Laboratory of Agricultural Microbiology, State Key Laboratory of Crop Biology, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, Shandong Agricultural University, 61 Daizong Street, Tai'an, 271018, China.

Background: Paenibacillus polymyxa is a typical plant growth-promoting rhizobacterium (PGPR), and synthesis of indole-3-acetic acid (IAA) is one of the reasons for its growth-promoting capacity. The synthetic pathways of IAA in P. polymyxa must be identified and modified.

Results: P. polymyxa SC2 and its spontaneous mutant SC2-M1 could promote plant growth by directly secreting IAA. Through metabonomic and genomic analysis, the genes patA, ilvB3, and fusE in the native IPyA pathway of IAA synthesis in strain SC2-M1 were predicted. A novel strong promoter P was rationally selected, synthetically analyzed, and then evaluated on its ability to express IAA synthetic genes. Co-expression of three genes, patA, ilvB3, and fusE, increased IAA yield by 60% in strain SC2-M1. Furthermore, the heterogeneous gene iaam of the IAM pathway and two heterogeneous IPyA pathways of IAA synthesis were selected to improve the IAA yield of strain SC2-M1. The genes ELJP6_14505, ipdC, and ELJP6_00725 of the entire IPyA pathway from Enterobacter ludwigii JP6 were expressed well by promoter P in strain SC2-M1 and increased IAA yield in the engineered strain SC2-M1 from 13 to 31 μg/mL, which was an increase of 138%.

Conclusions: The results of our study help reveal and enhance the IAA synthesis pathways of P. polymyxa and its future application.
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http://dx.doi.org/10.1186/s13068-022-02181-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367139PMC
August 2022

Engineering DNAzyme strategies for fluorescent detection of lead ions based on RNA cleavage-propelled signal amplification.

J Hazard Mater 2022 Aug 3;440:129712. Epub 2022 Aug 3.

Department of Food Quality and Safety, Jilin University, Changchun 130062, China. Electronic address:

Based on the high recognition ability and flexible programmability of GR5 DNAzyme, two fluorescent biosensors were engineered for amplified detection of Pb via incorporating TiCT MXenes and embedding 2-aminopurine (2-AP), respectively. The quencher-required approach relied on the DNA affinity and fluorescence quenching ability of TiCT MXenes. Benefiting from the low background signal modulated by TiCT MXenes, the sensitive determination of Pb was achieved in the linear range of 0.2-10 ng mL with the limit of detection (LOD) of 0.05 ng mL. The quencher-free approach combined the fluorescent trait of 2-AP embedded in DNA structure, and the RNA cleavage-propelled digestion process of Exonuclease I (Exo I) for signal amplification, indicating the sensitive detection of Pb with the LOD as low as 0.02 ng mL in the linear range of 0.1-10 ng mL. Both DNAzyme assays exhibited simple procedures, favorable specificity, rapid analysis, and satisfactory application in standard reference materials (lead in drinking water) and spiked water samples. The two fluorescent biosensors established in this work would not only provide theoretic fundament for DNA adsorption of TiCT MXenes and the design of 2-AP-embedded DNAzyme assays, but also hold a great potential for on-site monitoring of lead pollution in water samples.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129712DOI Listing
August 2022

Camrelizumab-induced anaphylactic shock in an esophageal squamous cell carcinoma patient: A case report and review of literature.

World J Clin Cases 2022 Jun;10(18):6198-6204

Department of Critical Care Medicine, Traditional Chinese Hospital of Lu'an affiliated to Anhui University of Traditional Chinese Medicine, Lu'an 237000, Anhui Province, China.

Background: Camrelizumab (SHR-1210), an immune checkpoint inhibitor, is clinically used as a therapeutic option for various types of tumors. However, reports of adverse reactions associated with camrelizumab are gradually increasing. Anaphylactic shock due to camrelizumab has not been reported previously, until now. We report here, for the first time, a case of anaphylactic shock associated with camrelizumab in a patient with esophageal squamous cell carcinoma.

Case Summary: An 84-year-old male esophageal cancer patient received radiotherapy and chemotherapy 11 years ago. He was diagnosed with advanced esophageal squamous cell carcinoma with liver metastasis (TxN1M1) and received the first immunotherapy (camrelizumab 200 mg/each time, once every 3 wk) dose in December 2020, with no adverse reactions. Three weeks later, a generalized rash was noted on the chest and upper limbs; palpitations and breathing difficulties with a sense of dying occurred 10 min after the patient had been administered with the second camrelizumab therapy. Electrocardiograph monitoring revealed a 70 beats/min pulse rate, 69/24 mmHg (1 mmHg = 0.133 kPa) blood pressure, 28 breaths/min respiratory rate, and 86% pulse oximetry in room air. The patient was diagnosed with anaphylactic shock and was managed with intravenous fluid, adrenaline, dexamethasone sodium phosphate, calcium glucosate, and noradrenaline. Approximately 2 h after treatment, the patient's anaphylactic shock symptoms had been completely relieved.

Conclusion: Due to the widespread use of camrelizumab, attention should be paid to anti-programmed cell death 1 antibody therapy-associated hypersensitivity or anaphylactic shock.
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http://dx.doi.org/10.12998/wjcc.v10.i18.6198DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254201PMC
June 2022

Early prediction of noninvasive ventilation failure after extubation: development and validation of a machine-learning model.

BMC Pulm Med 2022 Aug 8;22(1):304. Epub 2022 Aug 8.

Department of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Noninvasive ventilation (NIV) has been widely used in critically ill patients after extubation. However, NIV failure is associated with poor outcomes. This study aimed to determine early predictors of NIV failure and to construct an accurate machine-learning model to identify patients at risks of NIV failure after extubation in intensive care units (ICUs).

Methods: Patients who underwent NIV after extubation in the eICU Collaborative Research Database (eICU-CRD) were included. NIV failure was defined as need for invasive ventilatory support (reintubation or tracheotomy) or death after NIV initiation. A total of 93 clinical and laboratory variables were assessed, and the recursive feature elimination algorithm was used to select key features. Hyperparameter optimization was conducted with an automated machine-learning toolkit called Neural Network Intelligence. A machine-learning model called Categorical Boosting (CatBoost) was developed and compared with nine other models. The model was then prospectively validated among patients enrolled in the Cardiac Surgical ICU of Zhongshan Hospital, Fudan University.

Results: Of 929 patients included in the eICU-CRD cohort, 248 (26.7%) had NIV failure. The time from extubation to NIV, age, Glasgow Coma Scale (GCS) score, heart rate, respiratory rate, mean blood pressure (MBP), saturation of pulse oxygen (SpO), temperature, glucose, pH, pressure of oxygen in blood (PaO), urine output, input volume, ventilation duration, and mean airway pressure were selected. After hyperparameter optimization, our model showed the greatest accuracy in predicting NIV failure (AUROC: 0.872 [95% CI 0.82-0.92]) among all predictive methods in an internal validation. In the prospective validation cohort, our model was also superior (AUROC: 0.846 [95% CI 0.80-0.89]). The sensitivity and specificity in the prediction group is 89% and 75%, while in the validation group they are 90% and 70%. MV duration and respiratory rate were the most important features. Additionally, we developed a web-based tool to help clinicians use our model.

Conclusions: This study developed and prospectively validated the CatBoost model, which can be used to identify patients who are at risk of NIV failure. Thus, those patients might benefit from early triage and more intensive monitoring.

Trial Registration: NCT03704324. Registered 1 September 2018, https://register.

Clinicaltrials: gov .
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http://dx.doi.org/10.1186/s12890-022-02096-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358918PMC
August 2022

Ultrasensitive Sub-monolayer Palladium Induced Chirality Switching and Topological Evolution of Skyrmions.

Nano Lett 2022 Aug 8. Epub 2022 Aug 8.

Department of Physics, Georgetown University, Washington, D.C. 20057, United States.

Chiral spin textures are fundamentally interesting, with promise for device applications. Stabilizing chirality is conventionally achieved by introducing Dzyaloshinskii-Moriya interaction (DMI) in asymmetric multilayers, where the thickness of each layer is at least a few monolayers. Here we report an ultrasensitive chirality switching in (Ni/Co) multilayer induced by capping with only 0.22 monolayer of Pd. Using spin-polarized low-energy electron microscopy, we monitor the gradual evolution of domain walls from left-handed to right-handed Néel walls and quantify the DMI induced by the Pd capping layer. We also observe the chiral evolution of a skyrmion during the DMI switching, where no significant topological protection is found as the skyrmion winding number varies. This corresponds to a minimum energy cost of <1 attojoule during the skyrmion chirality switching. Our results demonstrate the detailed chirality evolution within skyrmions during the DMI sign switching, which is relevant to practical applications of skyrmionic devices.
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http://dx.doi.org/10.1021/acs.nanolett.2c02043DOI Listing
August 2022

HQ2CL: A High-Quality Class Center Learning System for Deep Face Recognition.

IEEE Trans Image Process 2022 16;31:5359-5370. Epub 2022 Aug 16.

Benefited from the proposals of function losses margin-based, face recognition has achieved significant improvements in recent years. Those losses aim to increase the margin between the different identities to enhance the discriminability. Ideally, the class center of different identities is far from each other, and face samples are compact around the corresponding class center. Hence, it's very vital to produce a high-quality class center. However, the distribution of training sets determines the class center. With low-quality samples being in the majority, the class center would be close to the samples with little identity information. As a result, it would impair the discriminability of the learned model for those unseen samples. In this work, we propose a High-Quality Class Center Learning system (HQ2CL). This is an effective system and guides the class center to approach the high-quality samples to keep the discriminability. Specifically, HQ2CL introduces a quality-aware scale and margin layer for the identification loss and constructs a new high-quality center loss. We implement the proposed system without additional burden. And we present the experimental evaluation over different face benchmarks. The experimental results show the superiority of our proposed HQ2CL over the state-of-the-arts.
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http://dx.doi.org/10.1109/TIP.2022.3195638DOI Listing
August 2022

Erratum: Eightfold Degenerate Fermions in Two Dimensions [Phys. Rev. Lett. 127, 176401 (2021)].

Phys Rev Lett 2022 Jul;129(4):049901

This corrects the article DOI: 10.1103/PhysRevLett.127.176401.
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http://dx.doi.org/10.1103/PhysRevLett.129.049901DOI Listing
July 2022

Corrigendum: as a model to study the mechanism of nociception.

Front Physiol 2022 22;13:956551. Epub 2022 Jul 22.

Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and the Prevention and Treatment With Traditional Chinese Medicine Research in Gansu Colleges and University, Gansu University of Chinese Medicine, Lanzhou, China.

[This corrects the article DOI: 10.3389/fphys.2022.854124.].
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http://dx.doi.org/10.3389/fphys.2022.956551DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355665PMC
July 2022

Silencing TUFM Inhibits Development of Monocrotaline-Induced Pulmonary Hypertension by Regulating Mitochondrial Autophagy via AMPK/mTOR Signal Pathway.

Oxid Med Cell Longev 2022 27;2022:4931611. Epub 2022 Jul 27.

Cheeloo College of Medicine, Shandong University, Jinan City, Shandong Province, 250012, China.

Pulmonary arterial hypertension (PAH) is an extremely malignant cardiovascular disease which mainly involves the uncontrollable proliferation of the pulmonary arterial smooth muscular cells (PASMCs). Recent studies have confirmed that mitochondria play an important role in the pathogenesis of pulmonary hypertension through sensing cell hypoxia, energy metabolism conversion, and apoptosis. As a mitochondrial membrane protein, TUFM has been regarded to be related to mitochondrial autophagy (mitophagy), apoptosis, and oxidative stress. Considering these factors are closely associated with the pathogenesis of PAH, we hypothesize that TUFM might play a role in the development of PAH. Our preliminary examination has showed TUFM mainly expressed in the PASMCs, and the subsequent test indicated an increased TUFM expression in the SMCs of pulmonary arteriole in monocrotaline- (MCT-) induced PAH rat model compared with the normal rat. The TUFM knockdown (Sh-TUFM) or overexpressed (OE-TUFM) rats were used to establish PAH by treating with MCT. A notable lower pulmonary arterial systolic pressure together with slightly morphological changes of pulmonary arteriole was observed in the Sh-TUFM group compared with the single MCT-induced PAH group. Increased levels of P62 and Bax and reduced LC3II/I, BECN1, and Bcl2 were detected in the Sh-TUFM group, while the expressions of these proteins in the OE-TUFM group were contrast to the results of the Sh-TUFM group. To elucidate the possible mechanism underlying biological effect of TUFM in PAH, PASMCs were treated with silence or overexpression of TUFM and then exposed to hypoxia condition. An obviously high levels of P62 and Bax along with a decreased LC3 II/I, BECN1, ULK1, Atg12, Atg13, and Bcl2 levels were noticed in cells with silence of TUFM. Moreover, the phosphorylated AMPK and mTOR which was well known in mitophagy modulating vary by the alternation of TUFM. These observations suggested that TUFM silence inhibits the development of MCT-induced PAH via AMPK/mTOR pathway.
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http://dx.doi.org/10.1155/2022/4931611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348918PMC
August 2022

In vivo processing of digital information molecularly with targeted specificity and robust reliability.

Sci Adv 2022 Aug 5;8(31):eabo7415. Epub 2022 Aug 5.

Department of Chemistry, Tsinghua University, Beijing, China.

DNA has attracted increasing interest as an appealing medium for information storage. However, target-specific rewriting of the digital data stored in intracellular DNA remains a grand challenge because the highly repetitive nature and uneven guanine-cytosine content render the encoded DNA sequences poorly compatible with endogenous ones. In this study, a dual-plasmid system based on gene editing tools was introduced into to process information accurately. Digital data containing large repeat units in binary codes, such as text, codebook, or image, were involved in the realization of target-specific rewriting in vivo, yielding up to 94% rewriting reliability. An optical reporter was introduced as an advanced tool for presenting data processing at the molecular level. Rewritten information was stored stably and amplified over hundreds of generations. Our work demonstrates a digital-to-biological information processing approach for highly efficient data storage, amplification, and rewriting, thus robustly promoting the application of DNA-based information technology.
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http://dx.doi.org/10.1126/sciadv.abo7415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355361PMC
August 2022

Removal mechanisms of erythromycin by microalgae Chlorella pyrenoidosa and toxicity assessment during the treatment process.

Sci Total Environ 2022 Aug 1:157777. Epub 2022 Aug 1.

Co-Innovation center for sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Longpan Road 159, Nanjing 210037, China; National Positioning Observation Station of Hung-tse Lake Wetland Ecosystem in Jiangsu Province, Hongze, Jiangsu 223100, China.

Microalgae-based biotechnology for antibiotic removal has received increasing attention as an economical and green method. This study investigated the removal mechanism of erythromycin by Chlorella pyrenoidosa and its correlation with the ecotoxic responses of microalgae. The degradation products (DPs) were identified, and their toxicity was predicted. The results indicated that only 4.04 %, 6.28 % and 23.53 % of erythromycin were left after 21-day microalgae treatment in 0.1, 1.0 and 10 mg/L treatments, respectively. Biodegradation contributed 48.62-67.01 %, 16.67-52.32 % and 6.42-24.82 %, while abiotic degradation contributed 8.76-29.61 %, 5.19-41.39 %, and 16.55-51.22 % to erythromycin attenuation in 0.1, 1.0, and 10 mg/L treatments, respectively. The growth and physiological-biochemical parameters of microalgae were slightly affected in low concentration treatment, which may be the main reason that biodegradation was the prominent removal mechanism. By contrast, oxidative damage in high concentration treatment inhibited the cell growth and chlorophyll content of microalgae, which hindered erythromycin biodegradation. In addition, eleven erythromycin degradation products (DPs) were identified during microalgae treatment of 21 days. Seven DPs including DP717, DP715, DP701A, DP701B, DP657, DP643, and DP557, represented higher toxicity to aquatic organisms than erythromycin.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157777DOI Listing
August 2022

[Research progress on the role of connexin 43 phosphorylation and its signal pathway in the pathogenesis of sepsis].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2022 Jun;34(6):655-660

Three Wards of the Department of Critical Medicine, the Second Affiliated Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi, China. Corresponding author: Zhang Wenkai, Email:

Sepsis is the main cause of death in intensive care unit (ICU). Sepsis and septic shock seriously affect the prognosis of patients and increase the mortality and re-morbidity of patients. Early and timely intervention can reduce the mortality and recurrence rate of patients with sepsis. The occurrence of sepsis may be related to the phosphorylation of connexin 43 (Cx43), which needs to be realized through various signal pathways. The related sites of connexin 43 are phosphorylated through different signal pathways to achieve the precise regulation of sepsis, these sites may be related targets for the treatment of sepsis and provide a direction for accurate treatment of sepsis. This paper mainly analyzes the role of Cx43-related signal pathways such as protein kinase A (PKA), protein kinase C (PKC), mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) in the pathogenesis of sepsis.
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http://dx.doi.org/10.3760/cma.j.cn121430-20211101-01606DOI Listing
June 2022

Penicixanthene E, a new xanthene isolated from a mangrove-derived fungus Penicillium sp.

J Antibiot (Tokyo) 2022 Sep 2;75(9):526-529. Epub 2022 Aug 2.

Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning, 530200, PR China.

One new xanthene derivative, named penicixanthene E (1), together with one known compound 2, was isolated from the EtOAc extract of the endophytic fungus Penicillium sp. GXIMD 03101, which was identified from the mangrove Acanthus ilicifolius L. collected in the South China Sea. The structure of 1 was elucidated by 1D and 2D NMR spectral interpretation and HREISMS data. The absolute configurations of C-9 and C-11 in 1 were proposed based on electronic circular dichroism (ECD), but the configuration at C-3 in 1 was unassigned. Compound 1 represents a xanthene derivative that was first reported, in which carbon-carbon double bond has been reduced. The cytotoxic activities of all compounds were evaluated, the result showed that compound 1 has weak activity against pancreatic cancer SW1990.
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http://dx.doi.org/10.1038/s41429-022-00548-0DOI Listing
September 2022

Dedifferentiated Schwann cell-derived TGF-β3 is essential for the neural system to promote wound healing.

Theranostics 2022 18;12(12):5470-5487. Epub 2022 Jul 18.

Department of Plastic & Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.

Wound healing is among the most complicated physiological processes and requires the synchronization of various cell types with distinct roles to re-establish the condition of the original skin. Patients affected by peripheral neuropathies often experience failure to heal. Loss of Schwann cells (SCs), a crucial population of peripheral nervous system cells in skin, may contribute to chronic wounds. However, the role of SCs in wound healing are poorly understood. The activity of SCs was investigated by using a cell atlas of the wound healing process, which was generated by integrating single-cell RNA sequencing (scRNA-seq) libraries covering different states of mouse back skin. The results of in silico analysis were validated by cell culture and mouse model. Selective inhibitors and conditional RNAi by virus transfection were utilized to investigate the role of SCs in wound healing. Findings from mouse experiments were further verified in scRNA-seq analysis of diabetic patients. Our in silico analysis revealed the heterogeneous cellular components of skin and the dynamic interactions of neural crest derived cells (NCs) with other cell types. We found that SCs dedifferentiated at an early stage of wound repair with upregulated Wnt signaling. We also identified dedifferentiated SC (dSC) defect in diabetic wounds in both mouse and human. Wnt inhibition at the wound site repressed SC dedifferentiation, leading to defective repair. Furthermore, dSCs derived TGF-β3, which is context-dependent, promoted the migration of fibroblasts and keratinocytes. Moreover, TGF-β3 supplementation enhanced the healing of chronic wounds in diabetic mice with impaired SCs. Our study thus advances the understanding of the roles of neural-derived cells in skin regeneration and suggests a potential therapeutic strategy for wound healing disorders.
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http://dx.doi.org/10.7150/thno.72317DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330527PMC
August 2022

Genome-wide identification of long non-coding (lncRNA) in 's adaptability to resistant rice.

PeerJ 2022 25;10:e13587. Epub 2022 Jul 25.

Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan, China.

Background: The brown planthopper (BPH), (Stål), is a very destructive pest that poses a major threat to rice plants worldwide. BPH and rice have developed complex feeding and defense strategies in the long-term co-evolution.

Methods: To explore the molecular mechanism of BPH's adaptation to resistant rice varieties, the lncRNA expression profiles of two virulent BPH populations were analyzed. The RNA-seq method was used to obtain the lncRNA expression data in TN1 and YHY15.

Results: In total, 3,112 highly reliable lncRNAs in TN1 and YHY15 were identified. Compared to the expression profiles between TN1 and YHY15, 157 differentially expressed lncRNAs, and 675 differentially expressed mRNAs were identified. Further analysis of the possible regulation relationships between differentially expressed lncRNAs and differentially expressed mRNAs, identified three pair antisense targets, nine pair -regulation targets, and 3,972 pair co-expressed targets. Function enriched found arginine and proline metabolism, glutathione metabolism, and carbon metabolism categories may significantly affect the adaptability in BPH when it is exposed to susceptible and resistant rice varieties. Altogether, it provided scientific data for the study of lncRNA regulation of brown planthopper resistance to rice. These results are helpful in the development of new control strategies for host defense against BPH and breeding rice for high yield.
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http://dx.doi.org/10.7717/peerj.13587DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9332332PMC
July 2022

Exponential decrease of airborne microplastics: From megacity to open ocean.

Sci Total Environ 2022 Jul 29;849:157702. Epub 2022 Jul 29.

State Key Laboratory of Estuarine and Coastal Research, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China. Electronic address:

Atmospheric transport has been recognized as an important route for microplastics (MPs) entering the ocean since the early 2019s, yet little data of their distribution patterns in marine air are currently available. In this study, we conducted continuous measurements of atmospheric MPs in the marine boundary layer across the western Pacific Ocean. Results suggested that synthetic MPs comprised 25.89 % of all identified particles, with the most being cotton and cellulose (51.68 %). Research revealed that atmospheric synthetic microfibers (22.54 %) are higher than the proportion of the surface oceanic synthetic microfibers (8.20 %) in the recent study. Further, the size of airborne MP fibers over open ocean is probably not the limiting factor during long-range transport. The mean abundance of atmospheric MPs over the western Pacific Ocean during sampling period was 0.841 ± 0.698 items/100 m. Regression analysis revealed an exponential relationship between average MP abundance and average longitude of sampled stations, and the average abundance of airborne MPs in coastal megacity is three orders of magnitude higher than that in sampled marine air of western Pacific. This study provides a better understanding on the impact of atmospheric transport of MPs within the global plastic cycle.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157702DOI Listing
July 2022

Pedicle subtraction osteotomy for the corrective surgery of ankylosing spondylitis with thoracolumbar kyphosis: experience with 38 patients.

BMC Musculoskelet Disord 2022 Jul 30;23(1):731. Epub 2022 Jul 30.

Department of Spine Surgery, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, Xinjiang, China.

Objective: To evaluate the sagittal parameters and clinical outcome of pedicle subtraction osteotomy (PSO) for the treatment of ankylosing spondylitis (AS) combined with thoracolumbar kyphosis.

Methods: The clinical data of 38 patients with AS combined with thoracolumbar kyphosis who underwent PSO were enrolled and divided into the lumbar lordosis group and the lumbar kyphosis group according to the preoperative sagittal morphology. They were subdivided into the lumbar lordosis T12 group, lordosis L1 group, kyphosis L2 group, and kyphosis L3 group. The spine sagittal parameters were compared between the preoperative and the postoperative. Outcome evaluation was performed by the Japanese Orthopedic Association (JOA) score, visual analogue scale (VAS), and the Oswestry Disability Index (ODI).

Results: A total of 38 patients with AS combined with thoracolumbar kyphosis were successfully treated by PSO, with a mean follow-up time of 26.9 ± 11.9 months. There were 30 males and 8 females with a mean age of 41.6 ± 7.1 years. Twenty patients consisted in the lumbar lordosis group and 18 patients in the lumbar kyphosis group. GK, SVA, and CBVA were improved significantly (P < 0.05) at the final follow-up between the lumbar lordosis T12 group and the L1 group. Patients in the lumbar kyphosis L2 group and L3 group all received satisfactory, including LL, GK, and SVA (P < 0.05). There was no statistically significant difference in the preoperative TK, GK, SVA, PT, and PI between the lumbar lordosis and lumbar kyphosis groups (P > 0.05). Postoperative complications occurred in three cases.

Conclusion: PSO was a practical method for the treatment of patients with AS combined with thoracolumbar kyphosis. PSO at L3 was recommended to be selected for the lumbar kyphosis to obtain greater SVA correction. CBVA of single-segment PSO may be significantly lower than the two-segment PSO in the management of patients with kyphosis of lower CBVA.
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http://dx.doi.org/10.1186/s12891-022-05693-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9338567PMC
July 2022

Decreased expression of hypoxia-inducible factor 1α (HIF-1α) in cord blood monocytes under anoxia.

Pediatr Res 2022 Jul 29. Epub 2022 Jul 29.

Department of Neonatology, Heidelberg University Children's Hospital, Heidelberg, Germany.

Background: Infections are a major cause for morbidity and mortality in neonates; however, the underling mechanisms for increased infection susceptibility are incompletely understood. Hypoxia, which is present in inflamed tissues, has been identified as an important activation signal for innate immune cells in adults and is mainly mediated by hypoxia-inducible factor 1α (HIF-1α). Fetal tissue pO physiologically is low but rises immediately after birth.

Methods: In this study, the effect of low oxygen partial pressure (pO) on HIF-1α expression and its targets phagocytosis, reactive oxygen species (ROS) production and vascular endothelial growth factor (VEGF) secretion was compared in vitro between immune cells from adult peripheral blood and cord blood using anoxia, HIF-1α stabilizer desferroxamin (DFO) and E. coli as stimuli.

Results: We show that anoxia-induced HIF-1α protein accumulation, phagocytosis, ROS-production and VEGF-expression were greatly diminished in cord blood compared to adult cells. E. coli led to HIF-1α gene expression in adult and cord blood immune cells; however, cord blood cells failed to accumulate HIF-1α protein and VEGF upon E. coli stimulation.

Conclusions: Taken together, our results show a diminished activation of cord blood immune cells by low pO, which might contribute to impaired reactivity in the context of infection.

Impact: Neonatal immune cells do not accumulate HIF-1α under low oxygen partial pressure leading to decreased phagocytosis and decreased ROS production. We demonstrate a previously unknown mechanism of reduced activation of neonatal immune cells in the context of an inflammatory response. This could contribute to the increased susceptibility of newborns and preterm infants to infection.
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http://dx.doi.org/10.1038/s41390-022-02193-7DOI Listing
July 2022

Global review of macrolide antibiotics in the aquatic environment: Sources, occurrence, fate, ecotoxicity, and risk assessment.

J Hazard Mater 2022 Jul 19;439:129628. Epub 2022 Jul 19.

Co-Innovation center for sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Longpan Road 159, Nanjing 210037, China; National Positioning Observation Station of Hung-tse Lake Wetland Ecosystem in Jiangsu Province, Hongze, Jiangsu 223100, China.

The extensive use of macrolide antibiotics (MCLs) has led to their frequent detection in aquatic environments, affecting water quality and ecological health. In this study, the sources, global distribution, environmental fate, ecotoxicity and global risk assessment of MCLs were analyzed based on recently published literature. The results revealed that there are eight main sources of MCLs in the water environment. These pollution sources resulted in MCL detection at average or median concentrations of up to 3847 ng/L, and the most polluted water bodies were the receiving waters of wastewater treatment plants (WWTPs) and densely inhabited areas. Considering the environmental fate, adsorption, indirect photodegradation, and bioremoval may be the main attenuation mechanisms in natural water environments. N-demethylation, O-demethylation, sugar and side chain loss from MCL molecules were the main pathways of MCLs photodegradation. Demethylation, phosphorylation, N-oxidation, lactone ring hydrolysis, and sugar loss were the main biodegradation pathways. The median effective concentration values of MCLs for microalgae, crustaceans, fish, and invertebrates were 0.21, 39.30, 106.42, and 28.00 mg/L, respectively. MCLs induced the generation of reactive oxygen species, that caused oxidative stress to biomolecules, and affected gene expression related to photosynthesis, energy metabolism, DNA replication, and repair. Moreover, over 50% of the reported water bodies represented a medium to high risk to microalgae. Further studies on the development of tertiary treatment technologies for antibiotic removal in WWTPs, the combined ecotoxicity of antibiotic mixtures at environmental concentration levels, and the development of accurate ecological risk assessment models should be encouraged.
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http://dx.doi.org/10.1016/j.jhazmat.2022.129628DOI Listing
July 2022

Large magnetoelectric resistance in the topological Dirac semimetal α-Sn.

Sci Adv 2022 Jul 29;8(30):eabo0052. Epub 2022 Jul 29.

Department of Physics, Colorado State University, Fort Collins, CO 80523, USA.

The spin-momentum locking of surface states in topological materials can produce a resistance that scales linearly with magnetic and electric fields. Such a bilinear magnetoelectric resistance (BMER) effect offers a new approach for information reading and field sensing applications, but the effects demonstrated so far are too weak or for low temperatures. This article reports the first observation of BMER effects in topological Dirac semimetals; the BMER responses were measured at room temperature and were substantially stronger than those reported previously. The experiments used topological Dirac semimetal α-Sn thin films grown on silicon substrates. The films showed BMER responses that are 10 times larger than previously measured at room temperature and are also larger than those previously obtained at low temperatures. These results represent a major advance toward realistic BMER applications. Significantly, the data also yield the first characterization of three-dimensional Fermi-level spin texture of topological surface states in α-Sn.
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http://dx.doi.org/10.1126/sciadv.abo0052DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9337753PMC
July 2022

Altered Prefrontal Blood Flow Related With Mild Cognitive Impairment in Parkinson's Disease: A Longitudinal Study.

Front Aging Neurosci 2022 11;14:896191. Epub 2022 Jul 11.

Department of Neurology, Zhongshan Hospital, Fudan University, Shanghai, China.

Cognitive impairment is a common non-motor symptom in Parkinson's disease (PD), with executive dysfunction being an initial manifestation. We aimed to investigate whether and how longitudinal changes in the prefrontal perfusion correlate with mild cognitive impairment (MCI) in patients with PD. We recruited 49 patients with PD with normal cognition and 37 matched healthy control subjects (HCs). Patients with PD completed arterial spin labeling MRI (ASL-MRI) scans and a comprehensive battery of neuropsychological assessments at baseline (V0) and 2-year follow-up (V1). HCs completed similar ASL-MRI scans and neuropsychological assessments at baseline. At V1, 10 patients with PD progressed to MCI (converters) and 39 patients remained cognitively normal (non-converters). We examined differences in the cerebral blood flow (CBF) derived from ASL-MRI and neuropsychological measures (a) between patients with PD and HCs at V0 (effect of the disease), (b) between V1 and V0 in patients with PD (effect of the disease progression), and (c) between converters and non-converters (effect of the MCI progression) using -tests or ANOVAs with false discovery rate correction. We further analyzed the relationship between longitudinal CBF and neuropsychological changes using multivariate regression models with false discovery rate correction, focusing on executive functions. At V0, no group difference was found in prefrontal CBF between patients with PD and HCs, although patients with PD showed worse performances on executive function. At V1, patients with PD showed significantly reduced CBF in multiple prefrontal regions, including the bilateral lateral orbitofrontal, medial orbitofrontal, middle frontal, inferior frontal, superior frontal, caudal anterior cingulate, and rostral anterior cingulate. More importantly, converters showed a more significant CBF reduction in the left lateral orbitofrontal cortex than non-converters. From V0 to V1, the prolonged completion time of Trail Making Test-B (TMT-B) negatively correlated with longitudinal CBF reduction in the right caudal anterior cingulate cortex. The decreased accuracy of the Stroop Color-Word Test positively correlated with longitudinal CBF reduction in the left medial orbitofrontal cortex. In addition, at V1, the completion time of TMT-B negatively correlated with CBF in the left caudal anterior cingulate cortex. Our findings suggest that longitudinal CBF reduction in the prefrontal cortex might impact cognitive functions (especially executive functions) at the early stages of PD.
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http://dx.doi.org/10.3389/fnagi.2022.896191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9309429PMC
July 2022

Circ-NCX1 inhibits LPS-induced chondrocyte apoptosis by regulating the miR-133a/SIRT1 axis.

Kaohsiung J Med Sci 2022 Jul 27. Epub 2022 Jul 27.

Department of Orthopedics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an City, Shaanxi Province, China.

Osteoarthritis (OA) is a degenerative joint disease, which is characterized by the degeneration of articular cartilage, thickening of subchondral bone, and inflammation of the synovial membrane. In this study, we aimed to investigate the effects and underlying mechanisms of circ-NCX1 in lipopolysaccharide (LPS)-induced injury in SW1353 chondrocytes, an in vitro model of OA. The levels of circ-NCX1, miR-133a, and related apoptotic proteins were determined by RT-qPCR. MTT assay was used to evaluate the cell viability. The apoptosis was determined by flow cytometry, whereas the expression of apoptosis proteins was detected by Western blot. Immunofluorescence was used to detect cleaved caspase-3 expression in cells. Luciferase reporter assay was used to verify the interaction between circ-NCX1 and miR-133a, and between miR-133a and Silent information regulator 2 homolog 1 (Sirt1). The results showed that the overexpression of circ-NCX1 significantly upregulated the chondrocyte viability and proliferation, and alleviated apoptosis in LPS-induced SW1353 cells. Immunofluorescence results showed that the overexpression of circ-NCX1 significantly reduced expression of LPS-stimulated cleaved Caspase-3. The RT-qPCR results showed that the overexpression of circ-NCX1 inhibited mRNA levels of cleaved Caspase-3 and Bax, and promoted mRNA levels of Bcl-2. Luciferase reporter assay showed that circ-NCX1 targeted miR-133a, and miR-133a directly targeted the Sirt1. In addition, overexpression of circ-NCX1 inhibited chondrocyte apoptosis and promoted Akt phosphorylation via the miR-133a/Sirt1 axis in LPS-induced chondrocytes. In conclusion, circ-NCX1 may serve as an important regulator of LPS-induced chondrocyte apoptosis through the miR-133a/Sirt1 axis, and may be involved in the development of OA.
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http://dx.doi.org/10.1002/kjm2.12564DOI Listing
July 2022

Piezoelectric MEMS Mirror with Lissajous Scanning for Automobile Adaptive Laser Headlights.

Micromachines (Basel) 2022 Jun 25;13(7). Epub 2022 Jun 25.

Department of Mechanical Engineering, Sichuan University, Chengdu 610065, China.

The emergence of smart headlights with reconfigurable light distributions that provide optimal illumination, highlight road objects, and project symbols to communicate with traffic participants further enhances road safety. Integrating all these functions in a single headlight usually suffers from issues of bulky multi-functional add-on modules with high cost or the use of conventional spatial light modulators with low optical efficiency and complex thermal design requirements. This paper presents a novel laser headlight prototype based on biaxially resonant microelectromechanical systems (MEMS) mirror light modulator for mapping blue laser patterns on phosphor plate to create structured white illumination and tunable road projection. The proposed headlight prototype system enables reconfigurable light distribution by leveraging laser beam scanning with fewer back-end lens and simple thermal design requirements. Built with thin-film lead zirconate titanate oxide (PbZrTiO) actuators, the MEMS mirror achieved high-frequency biaxial resonance of 17.328 kHz, 4.81 kHz, and optical scan angle of 12.9°. The large mirror design of 2.0 mm facilitates more refined resolvable projection pixels, delivers more optical power, and provides moderate optical aperture to possibly serve as the common spatial light modulator of headlight and the light detection and ranging (LiDAR) towards all-in-one integration. The carefully designed bi-axial resonant frequency improves the device's robustness by offsetting the lowest eigenmode away from the vehicle vibration. By establishing the laser headlight prototype systems of both 1D and 2D scanning modes, a mathematical model of laser modulation and MEMS electrical control principles of Lissajous scanning are proposed to tune the projection pattern density and shapes. It laid the foundation for developing a laser scanning control system with more complex project functions and prompting the application of MEMS for compact headlight system that addresses night driving visibility, eliminates glare effect, and renders interactive projection capabilities.
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http://dx.doi.org/10.3390/mi13070996DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9319498PMC
June 2022

Magnetic Evaluation of Heat-Resistant Martensitic Steel Subjected to Microstructure Degradation.

Materials (Basel) 2022 Jul 13;15(14). Epub 2022 Jul 13.

Department of Mechanical and Energy Engineering, Southern University of Science and Technology, Shenzhen 518055, China.

The present paper investigates the use of the magnetic hysteresis loop technique to nondestructively evaluate microstructural degradation in heat-resistant martensitic (HRM) steels. The degradation impairs the safe operation of thermal power plants and it is thus essential to periodically assess it using nondestructive evaluation (NDE) techniques. In this contribution, HRM steels are thermally aged up to 16,000 h at 675 °C to simulate the microstructural degradation, then the changes in the magnetic coercivity, hardness, and microstructure are systematically characterized and the relations between them are determined. Both coercivity and hardness decrease with thermal aging duration, which can be interpreted in terms of the microstructure parameters' evolution based on the pinning of crystal defects on domain walls and dislocations. Coercivity and hardness share the same softening trend with aging time, and good linear relations between coercivity, hardness, and microstructure parameters are found. These results provide a key to understanding the magnetic parameter evolution in HRM steels and suggest the possibility of using magnetic technologies for the NDE of microstructure degradation in thermal power plants.
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http://dx.doi.org/10.3390/ma15144865DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317982PMC
July 2022

Using Potential Molecular Transformation To Understand the Molecular Trade-Offs in Soil Dissolved Organic Matter.

Environ Sci Technol 2022 Aug 26;56(16):11827-11834. Epub 2022 Jul 26.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, P. R. China.

Understanding the chemical composition and molecular transformation in soil dissolved organic matter (DOM) is important to the global carbon cycle. To address this issue, ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) was applied to investigate DOM molecules in 36 paddy soils collected from subtropical China. All the detected 7576 unique molecules were divided into seven compound groups, and nine trade-off relationships between different compound groups were revealed based on principal component analysis and Pearson's correlation. An optimized method was developed to evaluate all potential molecular transformations in DOM samples. The concept of thermodynamics was introduced to evaluate the identified molecular transformations and classify them as thermodynamically favorable (TFP) and thermodynamically limited (TLP) processes. Here, we first tried to understand the molecular trade-offs by using the potential molecular transformations. All the nine trade-offs could be explained by molecular transformations. Six trade-offs had bases of biochemical reactions, and the trade-off-related direct transformations could explain the content variations of carbohydrate-like, condensed aromatic-like, tannin-like, and lignin-like compounds in TLP. More reasonable explanations existed in the TLP rather than TFP, which demonstrated the critical role of external energy in the molecular transformation of soil DOM.
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http://dx.doi.org/10.1021/acs.est.2c01137DOI Listing
August 2022

Detection and Molecular Characterization of Porcine Parvovirus 7 in Eastern Inner Mongolia Autonomous Region, China.

Front Vet Sci 2022 6;9:930123. Epub 2022 Jul 6.

Preventive Veterinary Laboratory, College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, China.

Porcine parvoviruses (PPV) and porcine circoviruses type 2 (PCV2) are widespread in the pig population. Recently, it was suggested that PPV7 may stimulate PCV2 and PCV3 replication. The present study aimed to make detection and molecular characterization of PPV7 for the first time in eastern Inner Mongolia Autonomous Region, China. Twenty-seven of ninety-four samples (28.72%) and five in eight pig farms were PPV7 positive. Further detection showed that the co-infection rate of PPV7 and PCV2 was 20.21% (19/94), and 9.59% (9/94) for PPV7 and PCV3. In addition, the positive rate of PPV7 in PCV2 positive samples was higher than that in PCV2 negative samples, supporting that PCV2 could act as a co-factor for PPV7 infection. In total, four PPV7 strains were sequenced and designated as NM-14, NM-19, NM-4, and NM-40. The amplified genome sequence of NM-14 and NM-40 were 3,999nt in length, while NM-19 and NM-4 were 3,996nt with a three nucleotides deletion at 3,097-3,099, resulting in an amino acid deletion in the Cap protein. Phylogenetic analysis based on the capsid amino acid (aa) sequences showed that 52 PPV7 strains were divided into two clades, and the four PPV7 strains in this study were all clustered in clade 1. The genome and capsid amino acid sequence of the four PPV7 strains identified in this study shared 80.0-96.9% and 85.9-100% similarity with that of 48 PPV7 reference strains selected in NCBI. Simplot analysis revealed that NM-19 and NM-4 strains were probably produced by recombination of two PPV7 strains from China. The amino acid sequence alignment analysis of capsid revealed that the four PPV7 strains detected in Inner Mongolia had multiple amino acid mutations in the 6 B cell linear epitopes compared with the reference strains, suggesting that the four PPV7 strains may have different characteristics in receptor binding and immunogenicity. In summary, this paper reported the PPV7 infection and molecular characterization in the eastern of Inner Mongolia Autonomous Region for the first time, which is helpful to understand the molecular epidemic characteristics of PPV7.
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http://dx.doi.org/10.3389/fvets.2022.930123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9298536PMC
July 2022
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