Publications by authors named "Kai Li"

2,230 Publications

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Gallium (Ga)-strontium (Sr) layered double hydroxide composite coating on titanium substrates for enhanced osteogenic and antibacterial abilities.

J Biomed Mater Res A 2021 Jul 29. Epub 2021 Jul 29.

Laboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Bacterial infection and poor osteogenic capacity can result in the loosing or failure of titanium (Ti)-based implants in the clinic. Therefore, it is urgent to design an effective approach to enhance the osteogenic property and restrict bacterial activity. In this study, a layered double hydroxide (LDH) composed of Ga and Sr ions on Ti substrates by a hydrothermal method, then calcined in 250°C and denoted as LDH250. The scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) were confirmed that the LDH films were successfully formed on the Ti substrates. Importantly, the obtained LDH films can induce an alkaline microenvironment around the Ti surface and regulate the behaviors of osteogenic cells and bacteria. In vitro cellular experiments, the LDH250 can enhance the differentiation of both MC3T3-E1 cells and osteoblasts, stimulate alkaline phosphatase activity (ALP), collagen secretion, and mineralization levels. Meanwhile, antimicrobial assay against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) demonstrated that the LDH250 samples had strong antibacterial abilities, which attributed to the release profile of Ga could act as a "Trojan horse" to destroy the bacterial iron metabolism, inducing of local alkaline environment, and producing reactive oxygen species. Hence, this study provides an effective method for reducing antibacterial infection and enhancing the bone integrative capacity of Ti-based implants for orthopedic applications.
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http://dx.doi.org/10.1002/jbm.a.37284DOI Listing
July 2021

Nuclear factor-kappa B (NF-κB) decoy oligodeoxynucleotide-loaded poly lactic-co-glycolic acid (PLGA) nanospheres promote periodontal tissue healing after tooth replantation in rats.

J Periodontol 2021 Jul 28. Epub 2021 Jul 28.

Department of Orthodontic Science, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan.

Background: Excessive inflammation in the periodontal tissue after tooth replantation can lead to inflammatory root resorption and interrupt periodontal tissue regeneration. We tested the hypothesis that NF-κB decoy oligodeoxynucleotide-loaded poly lactic-co-glycolic acid nanospheres (NF-PLGA) inhibit excessive inflammation and promote healing of periodontal tissue after replantation in rats.

Methods: The upper right incisors of rats were extracted, immersed in different specific solutions, and replanted. The rats were euthanized at 7, 14, and 28 days after replantation. Morphological evaluation with micro-CT and histological assessment with hematoxylin and eosin and tartrate-resistant acid phosphatase (TRAP) staining was performed. Additionally, we examined the expression of interleukin (IL)-1β, IL-6, transforming growth factor-β1 (TGF-β1), and fibroblast growth factor-2 (FGF-2) in the periodontal ligament (PDL) by performing immunohistological assessment.

Results: The NF-PLGA group showed significantly greater dental root thickness than the other experimental groups. Root resorption was not observed after the application of NF-PLGA on day 7. The application of NF-PLGA also resulted in a significantly lower number of TRAP-positive osteoclasts on days 7 and 14 after replantation. Significantly lower expression of IL-1β and IL-6 and higher expression of TGF-β1 and FGF-2 were observed under the application of NF-PLGA in the PDL.

Conclusions: NF-PLGA promoted the healing process by inhibiting the initial excessive inflammatory response in the PDL, preventing root resorption, and promoting periodontal tissue regeneration. The findings also suggested that the PLGA nanospheres-mediated transfection of the decoy oligodeoxynucleotides can be useful for the clinical application of replanted tooth root surfaces. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/JPER.21-0134DOI Listing
July 2021

Moderately Crystalline Azine-Linked Covalent Organic Framework Membrane for Ultrafast Molecular Sieving.

ACS Appl Mater Interfaces 2021 Jul 28. Epub 2021 Jul 28.

School of Chemical Engineering, Zhengzhou University, Zhengzhou 450001, PR China.

Covalent organic frameworks are potential candidates for the preparation of advanced molecular separation membranes due to their porous structure, uniform aperture, and chemical stability. However, the fabrication of continuous COF membranes in a facile and mild manner remains a challenge. Herein, a continuous, defect-free, and flexible azine-linked ACOF-1 membrane was prepared on a hydrolyzed polyacrylonitrile (HPAN) substrate via interfacial polymerization (IP). A moderately crystalline COF ultrathin selective layer enabled ultrafast molecular sieving. The effect of synthesis parameters including precursor concentration, catalyst dosage, and reaction duration on the dye separation performance was investigated. The optimized membrane displayed an ultrahigh water permeance of 142 L m h bar together with favorable rejection (e.g., 99.2% for Congo red and 96.3% for methyl blue). The water permeance is 5-12 times higher than that of reported membranes with similar rejections. In addition, ACOF-1 membranes demonstrate outstanding long-term stability together with organic solvent and extreme pH resistance. Meanwhile, the membrane is suitable for removing dyes from salt solution products owing to their nonselective permeation for hydrated salt ions (<10.6%). The superior performance and the excellent chemical stability render the ACOF-1 membrane a satisfactory system for water purification.
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http://dx.doi.org/10.1021/acsami.1c06891DOI Listing
July 2021

Recombinant human thymosin beta-4 (rhTβ4) improved scalp condition and microbiome homeostasis in seborrheic dermatitis.

Microb Biotechnol 2021 Jul 28. Epub 2021 Jul 28.

Beijing Institute of Biotechnology, Beijing, China.

Seborrheic dermatitis (SD) is a recurrent common inflammatory skin disease that affects all ethnic groups in all regions worldwide. However, no specific treatment or preventive measure is yet available. Identifying effective treatments with acceptable safety and tolerability is desirable. In this study, scalp microbiota alterations were measured in SD, showing significantly greater abundance of Malassezia and Staphylococcus and diminished fungal and bacterial diversity compared with healthy controls. We investigated the benefit of a 4-week treatment with 0.5 mg ml recombinant human thymosin β4 (rhTβ4) gel or 2% ketoconazole lotion on the scalp condition of 71 patients with SD compared with 21 healthy individuals. Clinical assessment (Adherent Scalp Flaking Score, and the Maximum Erythema Area) and physiological conditions (transepidermal water loss, hydration, and sebum secretion) were evaluated. The rhTβ4 treatment provided significantly greater efficacy than ketoconazole and a sustained effect in the treatment of scalp SD. More importantly, rhTβ4 dramatically improved the microbiome homeostasis and prompted a shift of scalp microflora towards healthy composition, helping symptoms and ameliorating physiological conditions more effectively and durably than ketoconazole. Our research demonstrated the scalp microbe dysbiosis of SD and highlighted rhTβ4 as a promising therapeutic strategy in the prevention and treatment of SD.
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http://dx.doi.org/10.1111/1751-7915.13897DOI Listing
July 2021

Single-molecule imaging of chromatin remodelers reveals role of ATPase in promoting fast kinetics of target search and dissociation from chromatin.

Elife 2021 Jul 27;10. Epub 2021 Jul 27.

Department of Biology, Johns Hopkins University, Baltimore, United States.

Conserved ATP-dependent chromatin remodelers establish and maintain genome-wide chromatin architectures of regulatory DNA during cellular lifespan, but the temporal interactions between remodelers and chromatin targets have been obscure. We performed live-cell single-molecule tracking for RSC, SWI/SNF, CHD1, ISW1, ISW2, and INO80 remodeling complexes in budding yeast and detected hyperkinetic behaviors for chromatin-bound molecules that frequently transition to the free state for all complexes. Chromatin-bound remodelers display notably higher diffusion than nucleosomal histones, and strikingly fast dissociation kinetics with 4-7 s mean residence times. These enhanced dynamics require ATP binding or hydrolysis by the catalytic ATPase, uncovering an additional function to its established role in nucleosome remodeling. Kinetic simulations show that multiple remodelers can repeatedly occupy the same promoter region on a timescale of minutes, implicating an unending 'tug-of-war' that controls a temporally shifting window of accessibility for the transcription initiation machinery.
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http://dx.doi.org/10.7554/eLife.69387DOI Listing
July 2021

Light-Controlled Precise Delivery of NIR-Responsive Semiconducting Polymer Nanoparticles with Promoted Vascular Permeability.

Adv Healthc Mater 2021 Jul 26:e2100569. Epub 2021 Jul 26.

Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, 518055, China.

The endothelial barrier plays an essential role in health and disease by protecting organs from toxins while allowing nutrients to access the circulation. However, it is the major obstacle that limits the delivery of therapeutic drugs to the diseased tissue. Here, it is reported for the first time that near-infrared (NIR) laser pulses can transiently promote the delivery of semiconducting polymer nanoparticles passing the vascular barrier via photoacoustic-effect-induced accumulation, only by the aid of pulse laser irradiation. This strategy enables selective and substantial accumulation of the NIR-absorbing nanoparticles inside specific tissues, implying the discovery of an unprecedented approach for light-controlled nanoparticle delivery. Especially, the nanoparticle delivery in solid tumors by 10-min laser scanning is approximately six times higher than that of the enhanced permeability and retention (EPR) effect in 24 h under current experimental conditions. Further results confirm that this strategy facilitates substantial accumulation of nanoparticles in the mouse brain with intact skull. This approach thus opens a new door for tissue-specific delivery of nanomaterials with an unprecedented level of efficiency and precision.
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http://dx.doi.org/10.1002/adhm.202100569DOI Listing
July 2021

Molecular modification, structural characterization, and biological activity of xylans.

Carbohydr Polym 2021 Oct 26;269:118248. Epub 2021 May 26.

College of Light Industry and Food Engineering, Guangxi University, Guangxi, Nanning 530004, China; Collaborative Innovation Center for Guangxi Sugarcane Industry, Guangxi University, Nanning 530004, China. Electronic address:

The differences in the source and structure of xylans make them have various biological activities. However, due to their inherent structural limitations, the various biological activities of xylans are far lower than those of commercial drugs. Currently, several types of molecular modification methods have been developed to address these limitations, and many derivatives with specific biological activity have been obtained. Further research on structural characteristics, structure-activity relationship and mechanism of action is of great significance for the development of xylan derivatives. Therefore, the major molecular modification methods of xylans are introduced in this paper, and the primary structure and conformation characteristics of xylans and their derivatives are summarized. In addition, the biological activity and structure-activity relationship of the modified xylans are also discussed.
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http://dx.doi.org/10.1016/j.carbpol.2021.118248DOI Listing
October 2021

Molecular characteristic and pathogenicity analysis of a novel multiple recombinant ALV-K strain.

Vet Microbiol 2021 Jul 21;260:109184. Epub 2021 Jul 21.

Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin, 150069, PR China. Electronic address:

Avian leukosis virus (ALV) can induce various tumors and cause serious production problems. ALVs isolated from chickens were divided into six subgroups (A-J). In 2012, a strain of a putative novel subgroup of ALVs was isolated from Chinese native chickens in Jiangsu Province and named as ALV-K. In this study, three ALV-K strains (JS14LH01, JS13LH14, and JS15SG01) were isolated from chickens with suspected ALV infection in Jiangsu Province. Their complete genomes were amplified, sequenced, and analyzed systematically. The results showed that JS14LH01 and JS13LH14 were ALV-K and ALV-E recombinant strains. Whereas JS15SG01 is an ALV-K, ALV-E, and ALV-J multiple recombinant strain containing the U3 region of ALV-J. The pathogenicity test of JS15SG01 revealed that, compared with previous ALV-K strains, the viremia and viral shedding level of JS15SG01-infected chickens were significantly increased, reaching 100 % and 59 %, respectively. More important, JS15SG01 induced significant proliferation of gliocytes in the cerebral cortex of infected chickens, accompanied by the neurotropic phenomenon. This is the first report about a multiple recombinant ALV-K strain that could invade and injure the brain tissue of chickens in China. Our findings enriched the epidemiologic data of ALV and helped to reveal the evolution of ALV strains prevalent in chicken fields.
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http://dx.doi.org/10.1016/j.vetmic.2021.109184DOI Listing
July 2021

LncRNA HCP5 in hBMSC-derived exosomes alleviates myocardial ischemia reperfusion injury by sponging miR-497 to activate IGF1/PI3K/AKT pathway.

Int J Cardiol 2021 Jul 23. Epub 2021 Jul 23.

Department of Cardiothoracic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210000, Jiangsu Province, PR China. Electronic address:

Ischemia/reperfusion (I/R) injury is an inevitable process during heart transplant and suppressing I/R injury could greatly improve the survival rate of recipients. Mesenchymal stem cells (MSCs) have positive effects on I/R. We aimed to investigate the mechanisms underlying the protective roles of MSCs in I/R. Both cell model and rat model of myocardial I/R were used. MTT assay and flow cytometry were used to measure cell viability and apoptosis, respectively. QRT-PCR and western blotting were employed to measure levels of lncRNA HCP5 (HLA complex P5), miR-497, apoptosis-related proteins, and insulin-like growth factor (IGF1)/PI3K/AKT pathway. Dual luciferase assay was used to validate interactions of HCP5 and miR-497, miR-497 and IGF1. Echocardiography was performed to evaluate cardiac function of rats. Serum levels of CK-MB and LDH were measured. H&E and Masson staining were used to examine morphology of myocardial tissues. hBMSC-derived exosomes (hBMSC-Exos) increased the viability of cardiomyocytes following hypoxia/reperfusion (H/R) and decreased apoptosis. H/R diminished HCP5 expression in cardiomyocytes while hBMSC-Exos recovered the level. Overexpression of HCP5 in hBMSC-Exos further enhanced the protective effects in H/R while HCP5 knockdown suppressed. HCP5 directly bound miR-497 and miR-497 targeted IGF1. miR-497 mimics or si-IGF1 blocked the effects of HCP5 overexpression. Further, hBMSC-Exos alleviated I/R injury in vivo and knockdown of HCP5 in hBMSC-Exos decreased the beneficial effects. AntagomiR-497 blocked the effects of HCP5 knockdown. HCP5 from hBMSC-Exos protects cardiomyocytes against I/R injury via sponging miR-497 to disinhibit IGF1/PI3K/AKT pathway. These results shed light on mechanisms underlying the protective role of hBMSC-Exos in I/R.
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http://dx.doi.org/10.1016/j.ijcard.2021.07.042DOI Listing
July 2021

A cell wall-localized NLR confers resistance to Soybean mosaic virus by recognizing viral-encoded cylindrical inclusion protein.

Mol Plant 2021 Jul 21. Epub 2021 Jul 21.

National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Soybean-Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China. Electronic address:

Soybean mosaic virus (SMV) causes severe yield losses and seed quality reduction in soybean (Glycine max) production worldwide. Rsc4 from cultivar Dabaima is a dominant genetic locus for SMV resistance, and its mapping interval contains three Nucleotide-binding domain Leucine-rich Repeat containing (NLR) candidates (Rsc4-1, Rsc4-2, and Rsc4-3). The NLR-type resistant proteins were considered as important intracellular pathogen sensors in the previous studies. In this research, based on transient expression assay in Nicotiana benthamiana leaves, we found that the longest transcript of Rsc4-3 is sufficient to induce resistance response to SMV; and CRISPR/Cas9-mediated Rsc4-3 knockout in resistant cultivar Dabaima compromised the resistance. These indicate that Rsc4-3 confers resistance to SMV. Interestingly, Rsc4-3 encodes a cell wall localized NLR-type resistant protein (Rsc4-3). The internal polypeptide region responsible for apoplastic targeting of Rsc4-3 and the putative palmitoylation sites on the N-terminus are essential for the resistance response. Furthermore, we showed that viral-encoded cylindrical inclusion (CI) protein partially localizes to the cell wall and can interact with Rsc4-3. Virus-driven or transient expression of CI protein of avirulent SMV strains is enough to induce resistance response in the presence of Rsc4-3, suggesting that CI is the avirulent gene for Rsc4-3 mediated resistance. Our work exhibited a case of NLR recognizing virus in the apoplast and provided a simple and effective method for identifying resistant genes against SMV infection.
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http://dx.doi.org/10.1016/j.molp.2021.07.013DOI Listing
July 2021

Peripheral blood indices to predict PFS/OS with anlotinib as a subsequent treatment in advanced small-cell lung cancer.

Cancer Biol Med 2021 Jul 24. Epub 2021 Jul 24.

Department of Thoracic Oncology Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, Tianjin's Clinical Research Center for Cancer, Tianjin 300060, China.

Objective: In the phase II ALTER-1202 (NCT03059797) trial, anlotinib significantly improved progression-free survival (PFS) and overall survival (OS) in patients with advanced small-cell lung cancer (SCLC) who underwent at least 2 previous chemotherapy cycles, when compared with a placebo group. To identify potential factors for predicting efficacy and prognosis with anlotinib treatment, we analyzed hematological indices at baseline and adverse events (AEs) over the course of anlotinib treatment.

Methods: Data were collected from March 2017 to April 2019 from a randomized, double-blind, placebo-controlled, multicenter, phase II trial of anlotinib. Eligible patients were randomly assigned 2:1 to receive anlotinib or placebo until disease progression, intolerable toxicity, or withdrawal of consent. The patients received anlotinib (12 mg) or an analogue capsule (placebo) orally once daily for 14 days every 3 weeks. The hematological indices at baseline and AEs that occurred in the initial 2 treatment cycles were recorded. The Kaplan-Meier test and Cox regression model were used to assess survival differences.

Results: A total of 82 patients (81 patients with complete data) were randomly assigned to receive anlotinib, with 38 receiving a placebo as a control. Multivariate analysis indicated that an elevated neutrophil to lymphocyte ratio > 7.75 and lactate dehydrogenase > 254.65 U/L at baseline were independent risk factors for PFS; basal elevated aspartate aminotransferase > 26.75 U/L, neuron specific enolase > 18.64 ng/mL, and fibrinogen > 4.645 g/L were independent risk factors for OS. During treatment, elevated γ glutamyltransferase and hypophosphatemia were independent predictors for a poor PFS, and elevated γ-glutamyl transferase and hypercholesterolemia were independent factors for OS.

Conclusions: Our study preliminarily defined potential factors that affected the PFS and OS at baseline and during anlotinib treatment in patients with advanced SCLC. Our findings provide a basis for screening the dominant population and for dynamic efficacy monitoring with anlotinib therapy.
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http://dx.doi.org/10.20892/j.issn.2095-3941.2020.0727DOI Listing
July 2021

Identification of a Novel Cleavage Site and Confirmation of the Effectiveness of NgAgo Gene Editing on RNA Targets.

Mol Biotechnol 2021 Jul 23. Epub 2021 Jul 23.

Laboratory Medicine Center, Huazhong University of Science and Technology Union Shenzhen Hospital (Nanshan Hospital), Shenzhen, 518000, Guangdong, People's Republic of China.

Clusters of regularly interspaced short palindromic repeats (CRISPR)/Cas systems have a powerful ability to edit DNA and RNA targets. However, the need for a specific recognition site, protospacer adjacent motif (PAM), of the CRISPR/Cas system limits its application in gene editing. Some Argonaute (Ago) proteins have endonuclease functions under the guidance of 5' phosphorylated or hydroxylated guide DNA (gDNA). The NgAgo protein might perform RNA gene editing at 37 °C, suggesting its application in mammalian cells; however, its mechanisms are unclear. In the present study, the target of NgAgo in RNA was confirmed in vitro and in vivo. Then, an in vitro RNA cleavage system was designed and the cleavage site was verified by sequencing. Furthermore, NgAgo and gDNA were transfected into cells to cleave an intracellular target sequence. We demonstrated targeted degradation of GFP, HCV, and AKR1B10 RNAs in a gDNA-dependent manner by NgAgo both in vitro and in vivo, but no effect on DNA was observed. Sequencing demonstrated that the cleavage sites are located at the 3' of the target RNA which is recognized by 5' sequence of the gDNA. These results confirmed that NgAgo-gDNA cleaves RNA not DNA. We observed that the cleavage site is located at the 3' of the target RNA, which is a new finding that has not been reported in the past.
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http://dx.doi.org/10.1007/s12033-021-00372-1DOI Listing
July 2021

Tsc1 regulates tight junction independent of mTORC1.

Proc Natl Acad Sci U S A 2021 Jul;118(30)

State Key Laboratory of Organ Failure Research, Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China;

Tuberous sclerosis complex 1 (Tsc1) is a tumor suppressor that functions together with Tsc2 to negatively regulate the mechanistic target of rapamycin complex 1 (mTORC1) activity. Here, we show that Tsc1 has a critical role in the tight junction (TJ) formation of epithelium, independent of its role in Tsc2 and mTORC1 regulation. When an epithelial cell establishes contact with neighboring cells, Tsc1, but not Tsc2, migrates from the cytoplasm to junctional membranes, in which it binds myosin 6 to anchor the perijunctional actin cytoskeleton to β-catenin and ZO-1. In its absence, perijunctional actin cytoskeleton fails to form. In mice, intestine-specific or inducible, whole-body Tsc1 ablation disrupts adherens junction/TJ structures in intestine or skin epithelia, respectively, causing Crohn's disease-like symptoms in the intestine or psoriasis-like phenotypes on the skin. In patients with Crohn's disease or psoriasis, junctional Tsc1 levels in epithelial tissues are markedly reduced, concomitant with the TJ structure impairment, suggesting that Tsc1 deficiency may underlie TJ-related diseases. These findings establish an essential role of Tsc1 in the formation of cell junctions and underpin its association with TJ-related human diseases.
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http://dx.doi.org/10.1073/pnas.2020891118DOI Listing
July 2021

DNA-Grafted 3D Superlattice Self-Assembly.

Int J Mol Sci 2021 Jul 15;22(14). Epub 2021 Jul 15.

Shenzhen Research Institute of Nanjing University, Shenzhen 518000, China.

The exploitation of new methods to control material structure has historically been dominating the material science. The bottom-up self-assembly strategy by taking atom/molecule/ensembles in nanoscale as building blocks and crystallization as a driving force bring hope for material fabrication. DNA-grafted nanoparticle has emerged as a "programmable atom equivalent" and was employed for the assembly of hierarchically ordered three-dimensional superlattice with novel properties and studying the unknown assembly mechanism due to its programmability and versatility in the binding capabilities. In this review, we highlight the assembly strategies and rules of DNA-grafted three-dimensional superlattice, dynamic assembly by different driving factors, and discuss their future applications.
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http://dx.doi.org/10.3390/ijms22147558DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8306452PMC
July 2021

Efficient biosynthesis of D-allulose in Bacillus subtilis through D-psicose 3-epimerase translation modification.

Int J Biol Macromol 2021 Jul 20;187:1-8. Epub 2021 Jul 20.

College of Light Industry and Food Engineering, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, China; Sugar Industry Collaborative Innovation Center, Guangxi University, 100 Daxue Road, Nanning 530004, Guangxi, China. Electronic address:

The combined catalysis of glucose isomerase (GI) and D-psicose 3-epimerase (DPEase) provided a convenient route for the direct synthesis of D-allulose from d-glucose, whose cost is lower than d-fructose. In the present research, the weak activity of DPEase was the key rate-limiting step and resulted in the accumulation of d-fructose in engineered Bacillus subtilis. Then, the 5'-untranslated region (5'-UTR) structure of the mRNA translational initiation region was optimized for the precise control of DPEase expression. The manipulation of the 5'-UTR region promoted the accessibility to ribosome binding and the stability of mRNA, resulting in a maximum of 1.73- and 1.98-fold increase in DPEase activity and intracellular mRNA amount, respectively. Under the optimal catalytic conditions of 75 °C, pH 6.5, 110 g/L d-glucose, and 1 mmol/L Co, the reaction equilibrium time was reduced from 7.6 h to 6.1 h. We hope that our results could provide a facilitated strategy for large-scale production of D-allulose at low-cost.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.07.093DOI Listing
July 2021

NEU4 inhibits motility of HCC cells by cleaving sialic acids on CD44.

Oncogene 2021 Jul 19. Epub 2021 Jul 19.

School of Life Science and Technology, Harbin Institute of Technology, Harbin, Heilongjiang Provence, China, 150008.

Hepatocellular carcinoma (HCC) is an extremely metastatic tumor. Sialic acids (SAs) are associated with cancer development and metastasis. NEU4 is a sialidase that removes SAs from glycoconjugates, while the function of the NEU4 in HCC has not been clearly explored. In our research, we found the NEU4 expression was significantly down-regulated in HCC tissues, which was correlated with high grades and poor outcomes of HCC. The NEU4 expression could be regulated by histone acetylation. In the functional analysis of NEU4, the cell motility was inhibited when NEU4 was overexpressed, and restored when NEU4 expression was down-regulated. Similarly, NEU4 over-expressed HCC cells showed less metastasis in athymic nude mice. Further study revealed that NEU4 could inhibit cell migration by enzymatic decomposition of SAs. Our results verified a NEU4 active site (NEU4) and overexpressing inactivates NEU4 that weakens the inhibition ability to cell migration. Further, 70 kinds of specific interacting proteins of NEU4 including CD44 were identified through mass spectrum. Moreover, the α2,3-linked SAs on CD44 were decreased and the hyaluronic acid (HA) binding ability was increased when NEU4 over-expressed or activated. Additionally, the mutation of CD44 with six N-glycosylation sites showed less sensibility to NEU4 on cell migration compared with wild-type CD44. In summary, our results revealed the mechanism of low expression of NEU4 in HCC and its inhibitory effect on cell migration by removal of SAs on CD44, which may provide new treatment strategies to control the motility and metastasis of HCC.
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http://dx.doi.org/10.1038/s41388-021-01955-7DOI Listing
July 2021

Triple combination of natural microbial action, etching, and gas foaming to synthesize hierarchical porous carbon for efficient adsorption of VOCs.

Environ Res 2021 Jul 15;202:111687. Epub 2021 Jul 15.

School of Mechanical Engineering, Tianjin University of Commerce, Tianjin, 300134, PR China.

Fungi residue, vinasse, and biogas residue differ from general biomass waste due to natural microbial action. Microbial fermentation helps create natural channels for the permeation of activators and produces proteins for natural nitrogen doping. Inspired by these advantages on porous carbon synthesis, this study adopted dual activators of KOH and KHCO to synthesize porous carbon with different pore ratios for efficient adsorption of volatile organic compounds (VOCs). The fungi residue possessed the least lignin due to the most severe microbial action, contributing to the best pore structures after activation. The etching effect from potassium compounds and gas foaming from the carbonate decomposition contributed to creating hierarchical porous carbon with ultra-high surface area, ca. 1536.8-2326.5 m/g. However, KHCO addition also caused nitrogen erosion, such that lower adsorption capacity was attained even with a higher surface area when the mass ratio of KOH/KHCO decreased from 2.5:0.5 to 2:1. The maximum adsorption capacities of chlorobenzene (CB) and benzene (PhH) reached 594.0 and 394.3 mg/g, respectively. Pore structure variations after adsorption were evaluated by freeze treatment to discover the adsorption mechanism. The surface area after CB and PhH adsorption decreased 40.3% and 34.5%, respectively. Most of the mesopores might transform into micropores due to the mono/multilayer stacking of adsorbates. The VOC adsorption kinetics were simulated by the Pseudo-first- and -second-order models and Y-N model. This paper provides a new approach for high-value biomass waste utilization after microbial action to synthesize efficient adsorbents for VOCs.
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http://dx.doi.org/10.1016/j.envres.2021.111687DOI Listing
July 2021

Occurrence of hypertension during third-line anlotinib is associated with progression-free survival in patients with squamous cell lung cancer (SCC): A post hoc analysis of the ALTER0303 trial.

Thorac Cancer 2021 Jul 17. Epub 2021 Jul 17.

Department of Oncology, Linyi Cancer Hospital, Linyi, China.

Background: There is a lack of targeted therapeutic options for squamous cell lung cancer (SCC). Accelerated hypertension is an issue with many targeted therapies for lung cancer. This study aimed to analyze the efficacy of anlotinib, based on progression-free survival (PFS) and overall survival (OS) in patients with SCC, stratified by hypertension and Eastern Cooperative Oncology Group (ECOG) score.

Methods: This was a post hoc analysis of a multicenter, double-blind, phase III ALTER0303 randomized controlled trial. Only patients with SCC were included. The occurrence of hypertension during the study period was defined according to CTCAE 4.03. OS and PFS were the primary and secondary endpoints, respectively. The patients were stratified according to hypertension and ECOG score, respectively.

Results: The median PFS in the patients who developed hypertension was longer than in those who did not (7.2 (95% CI: 3.5-11.0) versus 3.2 (95% CI: 1.2-5.3) months, p = 0.001; HR (95% CI), 0.4 (0.2-0.8)). In the ECOG 0 patients, the median PFS in the patients who developed hypertension versus those who did not was 5.6 vs. 1.8 months, respectively (Figure 2(d)). In the ECOG 1 patients, the median PFS in the patients who developed hypertension versus those who did not was 7.0 (95% CI: 3.0-11.0) vs. 4.8 (95% CI: 1.2-8.5) months (p = 0.043). No statistically significant differences were found in OS in the stratified analyses.

Conclusions: The occurrence of hypertension might be a clinical indicator predicting the efficacy of third-line anlotinib treatment in patients with SCC.
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http://dx.doi.org/10.1111/1759-7714.14076DOI Listing
July 2021

Controllable wrinkling in a prestretched dielectric elastomer sheet with striped electrodes.

Phys Rev E 2021 Jun;103(6-1):063001

Department of Civil Engineering, Anhui Jianzhu University, Hefei, Anhui 230601, People's Republic of China.

Controllable wrinkling of dielectric elastomer (DE) sheets is often applied to achieve some special applications such as diffraction gratings, optical sensors, soft actuators, and adjustable wetting surfaces. It is required to precisely predict and control the threshold voltage and wavelength of wrinkling. In view of the weakness of loss of tension criterion, a nonlinear plate theory considering the bending energy of DE sheet is utilized to investigate the wrinkling phenomenon in a prestretched DE sheet with striped electrodes. The results show that the threshold voltage of wrinkling is bigger than the corresponding voltage obtained from loss of tension, which results from the fact that the bending energy has a certain inhibiting effect on wrinkling of the DE sheet. Furthermore, the threshold voltage and wavelength of wrinkling can be effectively regulated by controlling prestretch. The striped electrodes can also effectively control the threshold voltage and wavelength. Especially, there exists an optimal width ratio of electrode corresponding to the lowest threshold voltage. The proposed method can be used to predict and control the behavior of wrinkling in the engineering applications of DE structures.
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http://dx.doi.org/10.1103/PhysRevE.103.063001DOI Listing
June 2021

Knockdown of LncRNA LINC00958 Inhibits the Proliferation and Migration of NSCLC Cells by MiR-204-3p/KIF2A Axis.

Cell Transplant 2021 Jan-Dec;30:9636897211025500

Department of Laboratory, Second People's Hospital of Jiaozuo City, the First Affiliated Hospital of Henan Polytechnic University, Jiaozuo, Henan Province, China.

Non-small cell lung cancer (NSCLC) is the most common type of lung cancer. Increasing evidence suggests that long non-coding RNAs (lncRNAs) function in the tumorigenesis of NSCLC. LINC00958, a newly identified lncRNA, has been reported to be closely linked to tumorigenesis in several cancers. However, its specific role in NSCLC remains unclear. In this study, we determined the expression of LINC00958 in NSCLC by RT-qPCR analysis and evaluated cell proliferation and migration by CCK-8 and transwell assays, respectively. We established a xenograft tumor model to examine the effect of LINC00958 on tumor growth in vivo. Luciferase reporter assays were performed to determine the interaction between LINC00958 and miR-204-3p and the interaction between miR-204-3p and KIF2A. We found that LINC00958 was up-regulated in NSCLC tissues and cell lines. Down-regulation of LINC00958 inhibited cell proliferation and migration in vitro and suppressed tumor growth in vivo. Besides, miR-204-3p was identified as a target of LINC00958 and miR-204-3p inhibitor could reverse the inhibitory effect of LINC00958 knockdown on proliferation and migration of NSCLC cells. We also validated that KIF2A, a direct target of miR-204-3p, was responsible for the biological role of LINC00958. KIF2A antagonized the effect of miR-204-3p on NSCLC cell proliferation and migration and was regulated by LINC00958/miR-204-3p. Taken together, these data indicate that the LINC00958/miR-204-3p/KIF2A axis is critical for NSCLC progression, which might provide a potential therapeutic target of NSCLC.
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http://dx.doi.org/10.1177/09636897211025500DOI Listing
July 2021

Acoustic Properties of Metal-Organic Frameworks.

Research (Wash D C) 2021 1;2021:9850151. Epub 2021 Jun 1.

School of Materials Science and Engineering, Tianjin Key Lab of Metal and Molecule-Based Material Chemistry, Nankai University, Tianjin 300350, China.

Metal-organic frameworks (MOFs) have attracted significant attention in the past two decades due to their diverse physical properties and associated functionalities. Although numerous advances have been made, the acoustic properties of MOFs have attracted very little attention. Here, we systematically investigate the acoustic velocities and impedances of 19 prototypical MOFs first-principle calculations. Our results demonstrate that these MOFs exhibit a wider range of acoustic velocities, higher anisotropy, and lower acoustic impedances than their inorganic counterparts, which are ascribed to their structural diversity and anisotropy, as well as low densities. In addition, the piezoelectric properties, which are intimately related to the acoustic properties, were calculated for 3 MOFs density functional perturbation theory, which reveals that MOFs can exhibit significant piezoelectricity due to the ionic contribution. Our work provides a comprehensive study of the fundamental acoustic properties of MOFs, which could stimulate further interest in this new exciting field.
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http://dx.doi.org/10.34133/2021/9850151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254136PMC
June 2021

Asphyxia caused by delayed subglottic stenosis after neck trauma.

Forensic Sci Med Pathol 2021 Jul 14. Epub 2021 Jul 14.

Department of Forensic Medicine, Nanjing Medical University, Nanjing, 211166, Jiangsu, China.

Delayed subglottic stenosis (SGS) is an unusual complication. Here, we report a particular case of delayed SGS. A 17-year-old female suffered extensive injuries including severe neck trauma in a car accident, and complained of dyspnea after 30 days. Tracheal stenosis was observed by fiber optic bronchoscopy, but no specific treatment was administered to the patient. While being transferred to a tertiary hospital 3 days later, the patient fell into deep coma due to hypoxia, and died of hypoxic-ischemic encephalopathy and severe pulmonary infection in the intensive care unit (ICU) 58 days later. Postmortem autopsy and pathological investigation revealed tracheal stenosis 3.0 cm below the vocal cords with a diameter of 0.5 cm, which was caused by a cricoid cartilage fracture, fibrous tissue proliferation and inflammatory cell infiltration. We believed that external forces caused the cricoid fracture and mucosal damage, and after a month of fibrous repair, scar tissue formed the stenosis and caused her death. This report describes a rare condition in which slowly progressive intralaryngeal stenosis formation after external neck trauma could cause asphyxial death in a previously asymptomatic adult.
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http://dx.doi.org/10.1007/s12024-021-00391-zDOI Listing
July 2021

SLC7A11/xCT Prevents Cardiac Hypertrophy by Inhibiting Ferroptosis.

Cardiovasc Drugs Ther 2021 Jul 14. Epub 2021 Jul 14.

Department of Pathology, Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, Beijing Lab for Cardiovascular Precision Medicine, Capital Medical University, Beijing, China.

Purpose: Systemic hypertension may induce adverse hypertrophy of the left cardiac ventricle. Pathological cardiac hypertrophy is a common cause of heart failure. We investigated the significance of ferroptosis repressor xCT in hypertrophic cardiomyopathy.

Methods: xCT expression in angiotensin II (Ang II)-treated mouse hearts and rat cardiomyocytes was determined using qRT-PCR and Western blotting. Cardiac hypertrophy was induced by Ang II infusion in xCT knockout mice and their wildtype counterparts. Blood pressure, cardiac pump function, and pathological changes of cardiac remodeling were analyzed in these mice. Cell death, oxidative stress, and xCT-mediated ferroptosis were examined in Ang II-treated rat cardiomyocytes.

Results: After Ang II infusion, xCT was downregulated at day 1 but upregulated at day 14 at both mRNA and protein levels. It was also decreased in Ang II-treated cardiomyocytes, but not in cardiofibroblasts. Inhibition of xCT exacerbated cardiomyocyte hypertrophy and boosted the levels of ferroptosis biomarkers Ptgs2, malondialdehyde, and reactive oxygen species induced by Ang II, while overexpression of xCT opposed these detrimental effects. Furthermore, knockout of xCT aggravated Ang II-mediated mouse cardiac fibrosis, hypertrophy, and dysfunction. Ferrostatin-1, a ferroptosis inhibitor, alleviated the exacerbation of cardiomyocyte hypertrophy caused by inhibiting xCT in cultured rat cells or ablating xCT in mice.

Conclusion: xCT acts as a suppressor in Ang II-mediated cardiac hypertrophy by blocking ferroptosis. Positive modulation of xCT may therefore represent a novel therapeutic approach against cardiac hypertrophic diseases.
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http://dx.doi.org/10.1007/s10557-021-07220-zDOI Listing
July 2021

Evaluation of energy values of high-fiber dietary ingredients with different solubility fed to growing pigs using the difference and regression methods.

Anim Nutr 2021 Jun 20;7(2):569-575. Epub 2021 Apr 20.

State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

The objective of this study was to compare the energy values of high-fiber dietary ingredients with different solubility (sugar beet pulp [SBP] and defatted rice bran [DFRB]) in growing pigs using the difference and the regression methods. A total of 21 barrows (initial BW, 40.5 ± 1.2 kg) were assigned to 3 blocks with BW as a blocking factor, and each block was assigned to a 7 × 2 incomplete Latin square design with 7 diets and two 13-d experimental periods. The 7 experimental diets consisted of a corn-soybean meal basal diet and 6 additional diets containing 10%, 20%, or 30% SBP or DFRB in the basal diet, respectively. Each of the experimental periods lasted 12 d, with a 7 d dietary adaptation period followed by 5-d total fecal and urine collection. Results showed that the digestible energy (DE) and metabolizable energy (ME) of the SBP determined by the difference method with different inclusion levels (10%, 20%, or 30%) were 2,712 and 2,628 kcal/kg, 2,683 and 2,580 kcal/kg, and 2,643 and 2,554 kcal/kg DM basis, respectively. The DE and ME in the DFRB evaluated by the difference method with 3 different inclusion levels were 2,407 and 2,243 kcal/kg, 2,687 and 2,598 kcal/kg, and 2,630 and 2,544 kcal/kg DM basis, respectively. Different inclusion levels had no effects on the energy values of each test ingredient estimated by the difference method. The DE and ME of the SBP and the DFRB estimated by the regression method were 2,562 and 2,472 kcal/kg and 2,685 and 2,606 kcal/kg DM basis, respectively. The energy values of each ingredient determined by the regression method were similar to the values estimated by the difference method with the 20% or 30% inclusion level. However, the energy values of the SBP and DFRB estimated by the difference method with the 10% inclusion level were inconsistent with the values determined by the regression method ( < 0.05). In conclusion, the regression method was a robust indirect method to evaluate the energy values for high-fiber ingredients with different solubility in growing pigs. If the number of experimental animals was limited, the difference method with a moderate inclusion level (at least 20%) of the test high-fiber ingredient in the basal diet could be applied to substitute the regression method.
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http://dx.doi.org/10.1016/j.aninu.2020.07.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8245795PMC
June 2021

Analysis on the relationship between winter precipitation and the annual variation of horse stomach fly community in arid desert steppe, Northwest China (2007-2019).

Integr Zool 2021 Jul 13. Epub 2021 Jul 13.

Key Laboratory of Non-Invasive Research Technology for Endangered Species, School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, China.

Gasterophilus spp. have been found to be widespread in reintroduced Przewalski's horses in the Kalamaili Nature Reserve (Northwest China). However, data on the annual variation in Gasterophilus infections are lacking. To analyze the epidemiological features and determine the cause of the annual variation in Gasterophilus infections, we treated 110 Przewalski's horses with ivermectin and collected Gasterophilus larvae from fecal samples each winter from 2007 to 2019. All 110 Przewalski's horses studied were found to be infected by Gasterophilus spp., and a total of 141,379 larvae were collected. Six species of Gasterophilus were identified with the following prevalence: G. pecorum (100%), G. nasalis (96.36%), G. nigricornis (94.55%), G. haemorrhoidalis (56.36%), G. intestinalis (59.09%), and G. inermis (3.64%). The mean infection intensity of Gasterophilus spp. larvae in Przewalski's horses was 1,285 ± 653. G. pecorum (92.96 ± 6.71%) was the most abundant species. The intensity of Gasterophilus spp. (r = -0.561, p < 0.046) was significantly correlated with winter precipitation. Our findings confirmed that, in the Kalamaili Nature Reserve, gasterophilosis is a severe parasitic disease in Przewalski's horses. Winter precipitation at the beginning of the year can indirectly affect the intensity and composition of Gasterophilus spp. in Przewalski's horses at the end of the year. Therefore, the water-related ecological regulation should be carried out to help reduce the parasite infection of Przewalski's horses. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/1749-4877.12578DOI Listing
July 2021

Naringenin inhibits pro‑inflammatory cytokine production in macrophages through inducing MT1G to suppress the activation of NF‑κB.

Mol Immunol 2021 Jul 9;137:155-162. Epub 2021 Jul 9.

School of Life Science and Technology, Harbin Institute of Technology, Harbin, 150001, China. Electronic address:

Naringenin (Nar) is a flavanone that has been suggested to provide human health benefits such as anti-inflammatory, anti-oxidant and anti-cancer properties. However, the mechanisms underlying these benefits are complex and still not fully understood. In this study, we investigated the effect of Nar on the inflammatory response of macrophages and its underlying mechanism. In lipopolysaccharide (LPS)-stimulated human macrophages, Nar inhibited the activation of NF-κB pathway and suppressed the downstream expression of pro-inflammatory factors. In addition, Nar was also able to induce metallothionein 1 G (MT1G) expression, and the inhibitory effects of Nar on the production of pro-inflammatory cytokines was dependent on MT1G. Mechanistically, we found that MT1G-mediated inhibition of pro-inflammatory cytokines responses might be through repressing NF-κB activation via zinc chelation. Overall, this study reveals a novel mechanism of Nar on inflammatory responses, the suppression of NF-κB activation through upregulation of MT1G.
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http://dx.doi.org/10.1016/j.molimm.2021.07.003DOI Listing
July 2021

Differential Responses of Transplanted Stem Cells to Diseased Environment Unveiled by a Molecular NIR-II Cell Tracker.

Research (Wash D C) 2021 19;2021:9798580. Epub 2021 Jun 19.

Center for Molecular Imaging Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.

Stem cell therapy holds high promises in regenerative medicine. The major challenge of clinical translation is to precisely and quantitatively evaluate the in vivo cell distribution, migration, and engraftment, which cannot be easily achieved by current techniques. To address this issue, for the first time, we have developed a molecular cell tracker with a strong fluorescence signal in the second near-infrared (NIR-II) window (1,000-1,700 nm) for real-time monitoring of in vivo cell behaviors in both healthy and diseased animal models. The NIR-II tracker (CelTrac1000) has shown complete cell labeling with low cytotoxicity and profound long-term tracking ability for 30 days in high spatiotemporal resolution for semiquantification of the biodistribution of transplanted stem cells. Taking advantage of the unique merits of CelTrac1000, the responses of transplanted stem cells to different diseased environments have been discriminated and unveiled. Furthermore, we also demonstrate CelTrac1000 as a universal and effective technique for ultrafast real-time tracking of the cellular migration and distribution in a 100 m single-cell cluster spatial resolution, along with the lung contraction and heart beating. As such, this NIR-II tracker will shift the optical cell tracking into a single-cell cluster and millisecond temporal resolution for better evaluating and understanding stem cell therapy, affording optimal doses and efficacy.
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http://dx.doi.org/10.34133/2021/9798580DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8237598PMC
June 2021

Numerical Investigation of Fracturing of Coal with Cleats Caused by Supercritical CO Explosion.

ACS Omega 2021 Jul 24;6(26):16854-16868. Epub 2021 Jun 24.

School of Science, Qingdao University of Technology, Qingdao 266000, China.

The experimental and numerical responses of coal specimens were studied in this work. A supercritical CO explosion experiment was carried out on the coal specimens using an independently developed triaxial load platform. The characteristics of crack generation in the specimens were obtained for different initial stresses. For a better understanding of the influence of cleats in a coal seam, a MATLAB code is developed to identify the actual geometry of the cleat in the coal specimen images, enabling the geometric representation of coal with cleats. The fracturing of coal with cleats induced by supercritical CO explosion under initial stress is validated using a combination of smoothed particle hydrodynamics and the finite-element method. The cleat can absorb and reflect the stress wave and hinder the propagation of cracks. According to the transmittance of the stress wave, the density and size of cracks that propagate through the cleat, the influences of the dip angle of the cleat, the aperture of the cleat, and the distance from the cleat to the center of the blast hole are discussed. The results show that the greater the distance from the cleat to the center of the blast hole or the greater the aperture of the cleat, the greater the hindrance of the cleat to the propagation of the cracks. With the increase of the dip angle of the cleat, the hindrance of the cleat to crack propagation first increases and then decreases.
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http://dx.doi.org/10.1021/acsomega.1c01450DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264853PMC
July 2021

Accurate and Fast Image Denoising via Attention Guided Scaling.

IEEE Trans Image Process 2021 12;30:6255-6265. Epub 2021 Jul 12.

Image denoising is a classical topic yet still a challenging problem, especially for reducing noise from the texture information. Feature scaling (e.g., downscale and upscale) is a widely practice in image denoising to enlarge receptive field size and save resources. However, such a common operation would lose some visual informative details. To address those problems, we propose fast and accurate image denoising via attention guided scaling (AGS). We find that the main informative feature channel and visual primitives during the scaling should keep similar. We then propose to extract the global channel-wise attention to maintain main channel information. Moreover, we propose to collect global descriptors by considering the entire spatial feature. And we then distribute the global descriptors to local positions of the scaled feature, based on their specific needs. We further introduce AGS for adversarial training, resulting in a more powerful discriminator. Extensive experiments show the effectiveness of our proposed method, where we clearly surpass all the state-of-the-art methods on most popular synthetic and real-world denoising benchmarks quantitatively and visually. We further show that our network contributes to other high-level vision applications and improves their performances significantly.
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http://dx.doi.org/10.1109/TIP.2021.3093396DOI Listing
July 2021

Self-Guided Deep Multiview Subspace Clustering via Consensus Affinity Regularization.

IEEE Trans Cybern 2021 Jul 8;PP. Epub 2021 Jul 8.

Multiview subspace clustering (MVSC) leverages the complementary information among different views of multiview data and seeks a consensus subspace clustering result better than that using any individual view. Though proved effective in some cases, existing MVSC methods often obtain unsatisfactory results since they perform subspace analysis with raw features that are often of high dimensions and contain noises. To remedy this, we propose a self-guided deep multiview subspace clustering (SDMSC) model that performs joint deep feature embedding and subspace analysis. SDMSC comprehensively explores multiview data and strives to obtain a consensus data affinity relationship agreed by features from not only all views but also all intermediate embedding spaces. With more constraints being cast, the desirable data affinity relationship is supposed to be more reliably recovered. Besides, to secure effective deep feature embedding without label supervision, we propose to use the data affinity relationship obtained with raw features as the supervision signals to self-guide the embedding process. With this strategy, the risk that our deep clustering model being trapped in bad local minima is reduced, bringing us satisfactory clustering results in a higher possibility. The experiments on seven widely used datasets show the proposed method significantly outperforms the state-of-the-art clustering methods. Our code is available at https://github.com/kailigo/dmvsc.git.
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http://dx.doi.org/10.1109/TCYB.2021.3087746DOI Listing
July 2021
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