Kai Johnsson

Mitochondrial NAD Controls Nuclear ARTD1-Induced ADP-Ribosylation.

Authors:

Ann-Katrin Hopp Federico Teloni Lavinia Bisceglie Corentin Gondrand Fabio Raith Kathrin Nowak Lukas Muskalla Anna Howald Patrick G A Pedrioli Kai Johnsson Matthias Altmeyer Deena M Leslie Pedrioli Michael O Hottiger

Mol Cell 2021 Jan 13. Epub 2021 Jan 13.

Department of Molecular Mechanisms of Disease (DMMD), University of Zurich, 8057 Zurich, Switzerland. Electronic address:

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January 2021

Author Correction: Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics.

Nat Commun 2020 Oct 9;11(1):5160. Epub 2020 Oct 9.

Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.

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October 2020

6,11-Dioxobenzo[]pyrido[1,2-]indoles Kill by Targeting Iron-Sulfur Protein Rv0338c (IspQ), A Putative Redox Sensor.

Authors:

Rita Székely Monica Rengifo-Gonzalez Vinayak Singh Olga Riabova Andrej Benjak Jérémie Piton Mena Cimino Etienne Kornobis Valerie Mizrahi Kai Johnsson Giulia Manina Vadim Makarov Stewart T Cole

ACS Infect Dis 2020 11 1;6(11):3015-3025. Epub 2020 Oct 1.

Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

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November 2020

SLC25A51 is a mammalian mitochondrial NAD transporter.

Authors:

Timothy S Luongo Jared M Eller Mu-Jie Lu Marc Niere Fabio Raith Caroline Perry Marc R Bornstein Paul Oliphint Lin Wang Melanie R McReynolds Marie E Migaud Joshua D Rabinowitz F Brad Johnson Kai Johnsson Mathias Ziegler Xiaolu A Cambronne Joseph A Baur

Nature 2020 12 9;588(7836):174-179. Epub 2020 Sep 9.

Department of Physiology and Institute for Diabetes, Obesity, and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

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December 2020

Environmentally Sensitive Color-Shifting Fluorophores for Bioimaging.

Authors:

Lu Wang Julien Hiblot Christoph Popp Lin Xue Kai Johnsson

Angew Chem Int Ed Engl 2020 12 28;59(49):21880-21884. Epub 2020 Sep 28.

Department of Chemical Biology, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany.

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December 2020

A biosensor for measuring NAD levels at the point of care.

Authors:

Qiuliyang Yu Narges Pourmandi Lin Xue Corentin Gondrand Sebastian Fabritz Daniel Bardy Luc Patiny Elena Katsyuba Johan Auwerx Kai Johnsson

Nat Metab 2019 12 13;1(12):1219-1225. Epub 2019 Dec 13.

Department of Chemical Biology, Max Planck Institute for Medical Research, Heidelberg, Germany.

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December 2019

Activatable fluorescent probes for hydrolase enzymes based on coumarin-hemicyanine hybrid fluorophores with large Stokes shifts.

Authors:

Hiroyoshi Fujioka Shin-Nosuke Uno Mako Kamiya Ryosuke Kojima Kai Johnsson Yasuteru Urano

Chem Commun (Camb) 2020 May;56(42):5617-5620

Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. and Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan and CREST (Japan) Agency for Medical Research and Development (AMED), 1-7-1 Otemachi, Chiyoda-ku, Tokyo 100-0004, Japan.

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May 2020

The RESOLUTE consortium: unlocking SLC transporters for drug discovery.

Authors:

Giulio Superti-Furga Daniel Lackner Tabea Wiedmer Alvaro Ingles-Prieto Barbara Barbosa Enrico Girardi Ulrich Goldmann Bettina Gürtl Kristaps Klavins Christoph Klimek Sabrina Lindinger Eva Liñeiro-Retes André C Müller Svenja Onstein Gregor Redinger Daniela Reil Vitaly Sedlyarov Gernot Wolf Matthew Crawford Robert Everley David Hepworth Shenping Liu Stephen Noell Mary Piotrowski Robert Stanton Hui Zhang Salvatore Corallino Andrea Faedo Maria Insidioso Giovanna Maresca Loredana Redaelli Francesca Sassone Lia Scarabottolo Michela Stucchi Paola Tarroni Sara Tremolada Helena Batoulis Andreas Becker Eckhard Bender Yung-Ning Chang Alexander Ehrmann Anke Müller-Fahrnow Vera Pütter Diana Zindel Bradford Hamilton Martin Lenter Diana Santacruz Coralie Viollet Charles Whitehurst Kai Johnsson Philipp Leippe Birgit Baumgarten Lena Chang Yvonne Ibig Martin Pfeifer Jürgen Reinhardt Julian Schönbett Paul Selzer Klaus Seuwen Charles Bettembourg Bruno Biton Jörg Czech Hélène de Foucauld Michel Didier Thomas Licher Vincent Mikol Antje Pommereau Frédéric Puech Veeranagouda Yaligara Aled Edwards Brandon J Bongers Laura H Heitman Ad P IJzerman Huub J Sijben Gerard J P van Westen Justine Grixti Douglas B Kell Farah Mughal Neil Swainston Marina Wright-Muelas Tina Bohstedt Nicola Burgess-Brown Liz Carpenter Katharina Dürr Jesper Hansen Andreea Scacioc Giulia Banci Claire Colas Daniela Digles Gerhard Ecker Barbara Füzi Viktoria Gamsjäger Melanie Grandits Riccardo Martini Florentina Troger Patrick Altermatt Cédric Doucerain Franz Dürrenberger Vania Manolova Anna-Lena Steck Hanna Sundström Maria Wilhelm Claire M Steppan

Nat Rev Drug Discov 2020 07;19(7):429-430

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July 2020

Chemogenetic Control of Nanobodies.

Authors:

Helen Farrants Miroslaw Tarnawski Thorsten G Müller Shotaro Otsuka Julien Hiblot Birgit Koch Moritz Kueblbeck Hans-Georg Kräusslich Jan Ellenberg Kai Johnsson

Nat Methods 2020 03 17;17(3):279-282. Epub 2020 Feb 17.

Department of Chemical Biology, Max Planck Institute for Medical Research, Heidelberg, Germany.

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March 2020

Super-resolution microscopy compatible fluorescent probes reveal endogenous glucagon-like peptide-1 receptor distribution and dynamics.

Nat Commun 2020 01 24;11(1):467. Epub 2020 Jan 24.

Institute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.

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January 2020

A ligand-based system for receptor-specific delivery of proteins.

Authors:

Mariano Maffei Chiara Morelli Ellie Graham Stefano Patriarca Laura Donzelli Balint Doleschall Fernanda de Castro Reis Linda Nocchi Cora H Chadick Luc Reymond Ivan R Corrêa Kai Johnsson Jamie A Hackett Paul A Heppenstall

Sci Rep 2019 12 16;9(1):19214. Epub 2019 Dec 16.

European Molecular Biology Laboratory (EMBL) Rome, Adriano Buzzati-Traverso Campus, 00015, Monterotondo, Italy.

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December 2019

A general strategy to develop cell permeable and fluorogenic probes for multicolour nanoscopy.

Authors:

Lu Wang Mai Tran Elisa D'Este Julia Roberti Birgit Koch Lin Xue Kai Johnsson

Nat Chem 2020 02 2;12(2):165-172. Epub 2019 Dec 2.

Department of Chemical Biology, Max Planck Institute for Medical Research, Heidelberg, Germany.

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February 2020

Photoactivation of silicon rhodamines via a light-induced protonation.

Authors:

Michelle S Frei Philipp Hoess Marko Lampe Bianca Nijmeijer Moritz Kueblbeck Jan Ellenberg Hubert Wadepohl Jonas Ries Stefan Pitsch Luc Reymond Kai Johnsson

Nat Commun 2019 10 8;10(1):4580. Epub 2019 Oct 8.

Department of Chemical Biology, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany.

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October 2019

Publisher Correction: The metabolite BH4 controls T cell proliferation in autoimmunity and cancer.

Nature 2019 Aug;572(7769):E18

IMBA, Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.

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August 2019

A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons.

Authors:

Mayya Sundukova Efthymia Prifti Annalisa Bucci Kseniia Kirillova Joana Serrao Luc Reymond Miwa Umebayashi Ruud Hovius Howard Riezman Kai Johnsson Paul A Heppenstall

Angew Chem Int Ed Engl 2019 02 25;58(8):2341-2344. Epub 2019 Jan 25.

Molecular Medicine Partnership Unit (MMPU), 69117, Heidelberg, Germany.

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February 2019

Unintended specificity of an engineered ligand-binding protein facilitated by unpredicted plasticity of the protein fold.

Authors:

Austin L Day Per Greisen Lindsey Doyle Alberto Schena Nephi Stella Kai Johnsson David Baker Barry Stoddard

Protein Eng Des Sel 2018 10;31(10):375-387

Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., Seattle, WA, USA.

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October 2018

Small-Molecule Fluorescent Probes for Live-Cell Super-Resolution Microscopy.

Authors:

Lu Wang Michelle S Frei Aleksandar Salim Kai Johnsson

J Am Chem Soc 2019 02 29;141(7):2770-2781. Epub 2019 Jan 29.

Department of Chemical Biology , Max Planck Institute for Medical Research , Jahnstrasse 29 , 69120 Heidelberg , Germany.

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February 2019

The metabolite BH4 controls T cell proliferation in autoimmunity and cancer.

Nature 2018 11 7;563(7732):564-568. Epub 2018 Nov 7.

IMBA, Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.

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November 2018

Semisynthetic sensor proteins enable metabolic assays at the point of care.

Authors:

Qiuliyang Yu Lin Xue Julien Hiblot Rudolf Griss Sebastian Fabritz Clothilde Roux Pierre-Alain Binz Dorothea Haas Jürgen G Okun Kai Johnsson

Science 2018 09;361(6407):1122-1126

Department of Chemical Biology, Max Planck Institute for Medical Research, 69120 Heidelberg, Germany.

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September 2018

SNAP-Tagged Nanobodies Enable Reversible Optical Control of a G Protein-Coupled Receptor via a Remotely Tethered Photoswitchable Ligand.

Authors:

Helen Farrants Vanessa A Gutzeit Amanda Acosta-Ruiz Dirk Trauner Kai Johnsson Joshua Levitz Johannes Broichhagen

ACS Chem Biol 2018 09 7;13(9):2682-2688. Epub 2018 Sep 7.

Department of Chemical Biology , Max Planck Institute for Medical Research , Jahnstrasse 29 , 69120 Heidelberg , Germany.

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September 2018

A New Fluorogenic Small-Molecule Labeling Tool for Surface Diffusion Analysis and Advanced Fluorescence Imaging of β-Site Amyloid Precursor Protein-Cleaving Enzyme 1 Based on Silicone Rhodamine: SiR-BACE1.

Authors:

Sandra Karch Johannes Broichhagen Julia Schneider Daniel Böning Stephanie Hartmann Benjamin Schmid Philipp Tripal Ralf Palmisano Christian Alzheimer Kai Johnsson Tobias Huth

J Med Chem 2018 Jul 10;61(14):6121-6139. Epub 2018 Jul 10.

Institute of Physiology and Pathophysiology , Friedrich-Alexander-Universität Erlangen-Nürnberg , Universitaetsstrasse 17 , 91054 Erlangen , Germany.

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July 2018

Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides.

Authors:

Olivier Sallin Luc Reymond Corentin Gondrand Fabio Raith Birgit Koch Kai Johnsson

Elife 2018 05 29;7. Epub 2018 May 29.

École Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering, Lausanne, Switzerland.

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May 2018

Control of mechanical pain hypersensitivity in mice through ligand-targeted photoablation of TrkB-positive sensory neurons.

Authors:

Rahul Dhandapani Cynthia Mary Arokiaraj Francisco J Taberner Paola Pacifico Sruthi Raja Linda Nocchi Carla Portulano Federica Franciosa Mariano Maffei Ahmad Fawzi Hussain Fernanda de Castro Reis Luc Reymond Emerald Perlas Simone Garcovich Stefan Barth Kai Johnsson Stefan G Lechner Paul A Heppenstall

Nat Commun 2018 04 24;9(1):1640. Epub 2018 Apr 24.

Epigenetics and Neurobiology Unit, EMBL Rome, Via Ramarini 32, Monterotondo, 00015, Italy.

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April 2018

Sampling and energy evaluation challenges in ligand binding protein design.

Authors:

Jiayi Dou Lindsey Doyle Per Jr Greisen Alberto Schena Hahnbeom Park Kai Johnsson Barry L Stoddard David Baker

Protein Sci 2017 Dec 30;26(12):2426-2437. Epub 2017 Oct 30.

Institute for Protein Design, University of Washington, Seattle, Washington.

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December 2017

Luciferases with Tunable Emission Wavelengths.

Authors:

Julien Hiblot Qiuliyang Yu Marina D B Sabbadini Luc Reymond Lin Xue Alberto Schena Olivier Sallin Nicholas Hill Rudolf Griss Kai Johnsson

Angew Chem Int Ed Engl 2017 11 9;56(46):14556-14560. Epub 2017 Oct 9.

Ecole Polytechnique Fédérale de Lausanne, EPFL, Institute of Chemical Sciences and Engineering, ISIC, NCCR in Chemical Biology, 1015, Lausanne, Switzerland.

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November 2017

Computational design of environmental sensors for the potent opioid fentanyl.

Authors:

Matthew J Bick Per J Greisen Kevin J Morey Mauricio S Antunes David La Banumathi Sankaran Luc Reymond Kai Johnsson June I Medford David Baker

Elife 2017 09 19;6. Epub 2017 Sep 19.

Department of Biochemistry, University of Washington, Seattle, United States.

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September 2017

Differences in cisplatin distribution in sensitive and resistant ovarian cancer cells: a TEM/NanoSIMS study.

Authors:

Ronald F S Lee Tina Riedel Stéphane Escrig Catherine Maclachlan Graham W Knott Curt A Davey Kai Johnsson Anders Meibom Paul J Dyson

Metallomics 2017 10;9(10):1413-1420

Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

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October 2017

Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins.

Authors:

Silvia Scarabelli Kui Thong Tan Rudolf Griss Ruud Hovius Pier Luca D'Alessandro Thomas Vorherr Kai Johnsson

ACS Sens 2017 Aug 2;2(8):1191-1197. Epub 2017 Aug 2.

École Polytechnique Fédérale de Lausanne , Institute of Chemical Sciences and Engineering, Lausanne CH-1015, Switzerland.

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August 2017

Bioluminescent Antibodies for Point-of-Care Diagnostics.

Authors:

Lin Xue Qiuliyang Yu Rudolf Griss Alberto Schena Kai Johnsson

Angew Chem Int Ed Engl 2017 06 16;56(25):7112-7116. Epub 2017 May 16.

Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), NCCR in Chemical Biology, 1015, Lausanne, Switzerland.

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June 2017

Expression proteomics study to determine metallodrug targets and optimal drug combinations.

Authors:

Ronald F S Lee Alexey Chernobrovkin Dorothea Rutishauser Claire S Allardyce David Hacker Kai Johnsson Roman A Zubarev Paul J Dyson

Sci Rep 2017 05 8;7(1):1590. Epub 2017 May 8.

Institute of Chemical Sciences and Engineering, Swiss Federal Institute of Technology Lausanne (EPFL), CH-1015, Lausanne, Switzerland.

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May 2017

Highly Modular Bioluminescent Sensors for Small Molecules and Proteins.

Authors:

Qiuliyang Yu Rudolf Griss Alberto Schena Kai Johnsson

Methods Enzymol 2017 28;589:365-382. Epub 2017 Feb 28.

École Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Lausanne, Switzerland; École Polytechnique Fédérale de Lausanne (EPFL), Institute of Bioengineering, Lausanne, Switzerland; National Centre of Competence in Research (NCCR) Chemical Biology, Lausanne, Switzerland. Electronic address:

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August 2017

Rational Design and Applications of Semisynthetic Modular Biosensors: SNIFITs and LUCIDs.

Authors:

Helen Farrants Julien Hiblot Rudolf Griss Kai Johnsson

Methods Mol Biol 2017 ;1596:101-117

National Centre of Competence in Research (NCCR) Chemical Biology, Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, École Polytechnique Fédéralede Lausanne (EPFL), 1015, Lausanne, Switzerland.

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February 2018

Acetylated tubulin is essential for touch sensation in mice.

Elife 2016 12 13;5. Epub 2016 Dec 13.

EMBL Mouse Biology Unit, Monterotondo, Italy.

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December 2016

Chemical Genetic Screen Identifies Natural Products that Modulate Centriole Number.

Authors:

Michele Graciotti Zhou Fang Kai Johnsson Pierre Gönczy

Chembiochem 2016 11 23;17(21):2063-2074. Epub 2016 Sep 23.

Swiss Institute for Experimental Cancer Research (ISREC), Swiss Federal Institute of Technology Lausanne (EPFL), 1015, Lausanne, Switzerland.

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November 2016

Identification of aminopyrimidine-sulfonamides as potent modulators of Wag31-mediated cell elongation in mycobacteria.

Mol Microbiol 2017 01 29;103(1):13-25. Epub 2016 Oct 29.

Microbial Pathogenesis, Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

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January 2017

Fluorogenic Probes for Multicolor Imaging in Living Cells.

Authors:

Gražvydas Lukinavičius Luc Reymond Keitaro Umezawa Olivier Sallin Elisa D'Este Fabian Göttfert Haisen Ta Stefan W Hell Yasuteru Urano Kai Johnsson

J Am Chem Soc 2016 08 21;138(30):9365-8. Epub 2016 Jul 21.

Institute of Bioengineering, NCCR in Chemical Biology, Institute of Chemical Sciences and Engineering (ISIC), Ecole Polytechnique Fédérale de Lausanne (EPFL) , 1015 Lausanne, Switzerland.

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August 2016

A General Strategy for the Semisynthesis of Ratiometric Fluorescent Sensor Proteins with Increased Dynamic Range.

Authors:

Lin Xue Efthymia Prifti Kai Johnsson

J Am Chem Soc 2016 04 15;138(16):5258-61. Epub 2016 Apr 15.

Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, National Centre of Competence in Research (NCCR) Chemical Biology, École Polytechnique Fédérale de Lausanne (EPFL) , 1015 Lausanne, Switzerland.

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April 2016

Corrigendum: Modulating protein activity using tethered ligands with mutually exclusive binding sites.

Authors:

Alberto Schena Rudolf Griss Kai Johnsson

Nat Commun 2016 Feb 15;7:10758. Epub 2016 Feb 15.

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February 2016

Imaging and manipulating proteins in live cells through covalent labeling.

Authors:

Lin Xue Iuliia A Karpenko Julien Hiblot Kai Johnsson

Nat Chem Biol 2015 Dec 17;11(12):917-23. Epub 2015 Nov 17.

Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, National Center of Competence in Research (NCCR) in Chemical Biology, Lausanne, Switzerland.

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December 2015

Tetrahydrobiopterin Biosynthesis as a Potential Target of the Kynurenine Pathway Metabolite Xanthurenic Acid.

Authors:

Hirohito Haruki Ruud Hovius Miriam Grønlund Pedersen Kai Johnsson

J Biol Chem 2016 Jan 12;291(2):652-7. Epub 2015 Nov 12.

From the Institute of Chemical Sciences and Engineering, Institute of Bioengineering, National Centre of Competence in Research (NCCR) in Chemical Biology, École Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland

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January 2016

Correction: NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro.

Authors:

Ronald F S Lee Stéphane Escrig Marie Croisier Stéphanie Clerc-Rosset Graham W Knott Anders Meibom Curt A Davey Kai Johnsson Paul J Dyson

Chem Commun (Camb) 2015 Nov;51(92):16577

Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

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November 2015

NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro.

Authors:

Ronald F S Lee Stéphane Escrig Marie Croisier Stéphanie Clerc-Rosset Graham W Knott Anders Meibom Curt A Davey Kai Johnsson Paul J Dyson

Chem Commun (Camb) 2015 Nov;51(92):16486-9

Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

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November 2015

SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy.

Authors:

Gražvydas Lukinavičius Claudia Blaukopf Elias Pershagen Alberto Schena Luc Reymond Emmanuel Derivery Marcos Gonzalez-Gaitan Elisa D'Este Stefan W Hell Daniel Wolfram Gerlich Kai Johnsson

Nat Commun 2015 Oct 1;6:8497. Epub 2015 Oct 1.

Ecole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology, 1015 Lausanne, Switzerland.

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October 2015

Modulating protein activity using tethered ligands with mutually exclusive binding sites.

Authors:

Alberto Schena Rudolf Griss Kai Johnsson

Nat Commun 2015 Jul 22;6:7830. Epub 2015 Jul 22.

1] École Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering, Avenue Forel 2, EPFL SB ISIC LIP BCH-4303, CH-1015 Lausanne, Switzerland [2] École Polytechnique Fédérale de Lausanne, Institute of Bioengineering, CH-1015 Lausanne, Switzerland [3] National Centre of Competence in Research in Chemical Biology, CH-1015 Lausanne, Switzerland.

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July 2015

Reduction of Neuropathic and Inflammatory Pain through Inhibition of the Tetrahydrobiopterin Pathway.

Neuron 2015 Jun;86(6):1393-406

Kirby Neurobiology Center, Boston Children's Hospital and Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA. Electronic address:

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June 2015

Fluorescent labeling of SNAP-tagged proteins in cells.

Authors:

Gražvydas Lukinavičius Luc Reymond Kai Johnsson

Methods Mol Biol 2015 ;1266:107-18

Institute of Chemical Sciences and Engineering, NCCR Chemical Biology, École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.

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September 2015

Genetic targeting of chemical indicators in vivo.

Authors:

Guoying Yang Fernanda de Castro Reis Mayya Sundukova Sofia Pimpinella Antonino Asaro Laura Castaldi Laura Batti Daniel Bilbao Luc Reymond Kai Johnsson Paul A Heppenstall

Nat Methods 2015 Feb 8;12(2):137-9. Epub 2014 Dec 8.

1] Mouse Biology Unit, European Molecular Biology Laboratory (EMBL), Monterotondo, Italy. [2] Molecular Medicine Partnership Unit (MMPU), Heidelberg, Germany.

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February 2015

Fluorescence microscopy: strategic blinking.

Authors:

Gražvydas Lukinavičius Kai Johnsson

Nat Chem 2014 Aug;6(8):663-4

cole Polytechnique Fédérale de Lausanne (EPFL), Institute of Chemical Sciences and Engineering, NCCR Chemical Biology, 1015 Lausanne, Switzerland.

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August 2014

Bioluminescent sensor proteins for point-of-care therapeutic drug monitoring.

Authors:

Rudolf Griss Alberto Schena Luc Reymond Luc Patiny Dominique Werner Christine E Tinberg David Baker Kai Johnsson

Nat Chem Biol 2014 Jul 8;10(7):598-603. Epub 2014 Jun 8.

1] École Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering, Lausanne, Switzerland. [2] École Polytechnique Fédérale de Lausanne, Institute of Bioengineering, Lausanne, Switzerland. [3] National Centre of Competence in Research in Chemical Biology, Lausanne, Switzerland.

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July 2014

Fluorogenic probes for live-cell imaging of the cytoskeleton.

Authors:

Gražvydas Lukinavičius Luc Reymond Elisa D'Este Anastasiya Masharina Fabian Göttfert Haisen Ta Angelika Güther Mathias Fournier Stefano Rizzo Herbert Waldmann Claudia Blaukopf Christoph Sommer Daniel W Gerlich Hans-Dieter Arndt Stefan W Hell Kai Johnsson

Nat Methods 2014 Jul 25;11(7):731-3. Epub 2014 May 25.

1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2] National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland.

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July 2014

A fluorogenic probe for SNAP-tagged plasma membrane proteins based on the solvatochromic molecule Nile Red.

Authors:

Efthymia Prifti Luc Reymond Miwa Umebayashi Ruud Hovius Howard Riezman Kai Johnsson

ACS Chem Biol 2014 Mar 29;9(3):606-12. Epub 2014 Jan 29.

École Polytechnique Fédérale de Lausanne (EPFL) , Institute of Chemical Sciences and Engineering, National Centre of Competence in Research (NCCR) in Chemical Biology, 1015 Lausanne, Switzerland.

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March 2014

Sensing acetylcholine and anticholinesterase compounds.

Authors:

Alberto Schena Kai Johnsson

Angew Chem Int Ed Engl 2014 Jan 13;53(5):1302-5. Epub 2013 Dec 13.

Ecole Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, National Centre of Competence in Research (NCCR) in Chemical Biology, 1015 Lausanne (Switzerland).

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January 2014

Commercial Cdk1 antibodies recognize the centrosomal protein Cep152.

Authors:

Gražvydas Lukinavičius Darja Lavogina Pierre Gönczy Kai Johnsson

Biotechniques 2013 Sep;55(3):111-4

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September 2013

Yeast three-hybrid screening for identifying anti-tuberculosis drug targets.

Authors:

Simone Moser Kai Johnsson

Chembiochem 2013 Nov 16;14(17):2239-42. Epub 2013 Oct 16.

Institute of Chemical Science and Engineering, Institute of Bioengineering, National Centre of Competence in Research NCCR Chemical Biology, École Polytechnique Fédérale de Lausanne, EPFL SB ISIC LIP, 1015 Lausanne (Switzerland); Current address: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139 (USA).

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November 2013

Computational design of ligand-binding proteins with high affinity and selectivity.

Authors:

Christine E Tinberg Sagar D Khare Jiayi Dou Lindsey Doyle Jorgen W Nelson Alberto Schena Wojciech Jankowski Charalampos G Kalodimos Kai Johnsson Barry L Stoddard David Baker

Nature 2013 Sep 4;501(7466):212-216. Epub 2013 Sep 4.

Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.

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September 2013

Identification of a small molecule with activity against drug-resistant and persistent tuberculosis.

Proc Natl Acad Sci U S A 2013 Jul 17;110(27):E2510-7. Epub 2013 Jun 17.

Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.

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July 2013

Tetrahydrobiopterin biosynthesis as an off-target of sulfa drugs.

Authors:

Hirohito Haruki Miriam Grønlund Pedersen Katarzyna Irena Gorska Florence Pojer Kai Johnsson

Science 2013 May;340(6135):987-91

EPFL, Institute of Chemical Sciences and Engineering, Institute of Bioengineering, National Centre of Competence in Research in Chemical Biology, 1015 Lausanne, Switzerland.

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May 2013

Substrates for improved live-cell fluorescence labeling of SNAP-tag.

Authors:

Ivan R Corrêa Brenda Baker Aihua Zhang Luo Sun Christopher R Provost Gražvydas Lukinavičius Luc Reymond Kai Johnsson Ming-Qun Xu

Curr Pharm Des 2013 ;19(30):5414-20

New England Biolabs, Inc., 240 County Road, Ipswich, MA 01938, USA.

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March 2014

A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins.

Authors:

Gražvydas Lukinavičius Keitaro Umezawa Nicolas Olivier Alf Honigmann Guoying Yang Tilman Plass Veronika Mueller Luc Reymond Ivan R Corrêa Zhen-Ge Luo Carsten Schultz Edward A Lemke Paul Heppenstall Christian Eggeling Suliana Manley Kai Johnsson

Nat Chem 2013 Feb 6;5(2):132-9. Epub 2013 Jan 6.

Ecole Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering (ISIC), National Centre of Competence in Research (NCCR) in Chemical Biology, 1015 Lausanne, Switzerland.

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February 2013

Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 centrosomal complex.

Authors:

Gražvydas Lukinavičius Darja Lavogina Meritxell Orpinell Keitaro Umezawa Luc Reymond Nathalie Garin Pierre Gönczy Kai Johnsson

Curr Biol 2013 Feb 17;23(3):265-70. Epub 2013 Jan 17.

Institute of Chemical Sciences and Engineering (ISIC), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne 1015, Switzerland.

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February 2013

A fluorescent sensor for GABA and synthetic GABA(B) receptor ligands.

Authors:

Anastasiya Masharina Luc Reymond Damien Maurel Keitaro Umezawa Kai Johnsson

J Am Chem Soc 2012 Nov 5;134(46):19026-34. Epub 2012 Nov 5.

Institute of Chemical Sciences and Engineering (ISIC), Institute of Bioengineering, NCCR in Chemical Biology, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.

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November 2012

Investigating endocytic pathways to the endoplasmic reticulum and to the cytosol using SNAP-trap.

Authors:

Roger Geiger Stefania Luisoni Kai Johnsson Urs F Greber Ari Helenius

Traffic 2013 Jan 12;14(1):36-46. Epub 2012 Nov 12.

Institute of Biochemistry, ETH Zurich, Schafmattstrasse 18, Zurich, CH-8093, Switzerland.

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January 2013

WhiB5, a transcriptional regulator that contributes to Mycobacterium tuberculosis virulence and reactivation.

Authors:

Stefano Casonato Axel Cervantes Sánchez Hirohito Haruki Monica Rengifo González Roberta Provvedi Elisa Dainese Thomas Jaouen Susanne Gola Estela Bini Miguel Vicente Kai Johnsson Daniela Ghisotti Giorgio Palù Rogelio Hernández-Pando Riccardo Manganelli

Infect Immun 2012 Sep 25;80(9):3132-44. Epub 2012 Jun 25.

Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Padua, Italy.

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September 2012

Exploiting ligand-protein conjugates to monitor ligand-receptor interactions.

Authors:

Hirohito Haruki Monica Rengifo Gonzalez Kai Johnsson

PLoS One 2012 31;7(5):e37598. Epub 2012 May 31.

École Polytechnique Fédérale de Lausanne-EPFL, Institute of Chemical Sciences and Engineering, Institute of Bioengineering, National Centre of Competence in Research-NCCR in Chemical Biology, Lausanne, Switzerland.

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October 2012

Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma.

Authors:

Karolina Bojkowska Fabio Aloisio Marco Cassano Adamandia Kapopoulou Francesca Santoni de Sio Nadine Zangger Sandra Offner Cristina Cartoni Charles Thomas Simon Quenneville Kai Johnsson Didier Trono

Hepatology 2012 Oct 27;56(4):1279-90. Epub 2012 Aug 27.

School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

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October 2012

Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation.

Authors:

Gucci Jijuan Gu Mikaela Friedman Christian Jost Kai Johnsson Masood Kamali-Moghaddam Andreas Plückthun Ulf Landegren Ola Söderberg

N Biotechnol 2013 Jan 2;30(2):144-52. Epub 2012 Jun 2.

Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University, SE-75185 Uppsala, Sweden.

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January 2013

A semisynthetic fluorescent sensor protein for glutamate.

Authors:

Matthias A Brun Kui-Thong Tan Rudolf Griss Anna Kielkowska Luc Reymond Kai Johnsson

J Am Chem Soc 2012 May 25;134(18):7676-8. Epub 2012 Apr 25.

Institute of Chemical Sciences and Engineering, National Centre of Competence in Research Chemical Biology, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

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May 2012

Visualizing biochemical activities in living cells through chemistry.

Authors:

Luc Reymond Gražvydas Lukinavičius Keitaro Umezawa Damien Maurel Matthias A Brun Anastasiya Masharina Karolina Bojkowska Birgit Mollwitz Alberto Schena Rudolf Griss Kai Johnsson

Chimia (Aarau) 2011 ;65(11):868-71

Institute of Chemical Sciences and Engineering, NCCR Chemical Biology, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

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February 2012

Chemical biology approaches to membrane homeostasis and function.

Authors:

Miwa Takahashi-Umebayashi Ludovic Pineau Thomas Hannich Andreas Zumbuehl David Alonso Doval Stefan Matile Christian Heinis Gerardo Turcatti Robbie Loewith Aurélien Roux Luc Reymond Kai Johnsson Howard Riezman

Chimia (Aarau) 2011 ;65(11):849-52

Departments of Biochemistry, Organic Chemistry and Molecular Biology, University of Geneva, 30 quai Ernest Ansermet, CH-1211 Geneva 4, Switzerland.

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February 2012

NCCR Chemical Biology: Interdisciplinary Research Excellence, Outreach, Education, and New Tools for Switzerland.

Authors:

Susi Sturzenegger Kai Johnsson Howard Riezman

Chimia (Aarau) 2011 ;65(11):832-4

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February 2012

NCCR Chemical Biology: Interdisciplinary Research Excellence, Outreach, Education, and New Tools for Switzerland.

Authors:

Susi Sturzenegger Kai Johnsson Howard Riezman

Chimia (Aarau) 2011 ;65(11):832-4

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February 2012

Triplet imaging of oxygen consumption during the contraction of a single smooth muscle cell (A7r5).

Authors:

Matthias Geissbuehler Thiemo Spielmann Aurélie Formey Iwan Märki Marcel Leutenegger Boris Hinz Kai Johnsson Dimitri Van De Ville Theo Lasser

Adv Exp Med Biol 2012 ;737:263-8

Laboratoire d'Optique Biomédicale LOB, École Polytechnique Fédérale de Lausanne (EPFL), BM 5.138, Station 17, Lausanne 1015, Switzerland.

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June 2012

Triplet imaging of oxygen consumption during the contraction of a single smooth muscle cell (A7r5).

Authors:

Matthias Geissbuehler Thiemo Spielmann Aurélie Formey Iwan Märki Marcel Leutenegger Boris Hinz Kai Johnsson Dimitri Van De Ville Theo Lasser

Adv Exp Med Biol 2012 ;737:263-8

Laboratoire d'Optique Biomédicale LOB, École Polytechnique Fédérale de Lausanne (EPFL), BM 5.138, Station 17, Lausanne 1015, Switzerland.

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June 2012

Switchable fluorophores for protein labeling in living cells.

Authors:

Gražvydas Lukinavičius Kai Johnsson

Curr Opin Chem Biol 2011 Dec 11;15(6):768-74. Epub 2011 Nov 11.

Institute of Chemical Sciences and Engineering, NCCR Chemical Biology, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

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December 2011

A caged, localizable rhodamine derivative for superresolution microscopy.

Authors:

Sambashiva Banala Damien Maurel Suliana Manley Kai Johnsson

ACS Chem Biol 2012 Feb 4;7(2):289-93. Epub 2011 Nov 4.

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February 2012

Searching for the protein targets of bioactive molecules.

Authors:

Christopher Chidley Hirohito Haruki Miriam Grønlund Pedersen Cindy Fellay Simone Moser Kai Johnsson

Chimia (Aarau) 2011 ;65(9):720-4

Institute of Chemical Sciences and Engineering, NCCR Chemical Biology, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne.

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December 2011

Semisynthesis of fluorescent metabolite sensors on cell surfaces.

Authors:

Matthias A Brun Rudolf Griss Luc Reymond Kui-Thong Tan Joachim Piguet Ruud J R W Peters Horst Vogel Kai Johnsson

J Am Chem Soc 2011 Oct 16;133(40):16235-42. Epub 2011 Sep 16.

Institute of Chemical Sciences and Engineering, National Centre of Competence in Research Chemical Biology, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

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October 2011

Development of SNAP-tag fluorogenic probes for wash-free fluorescence imaging.

Authors:

Xiaoli Sun Aihua Zhang Brenda Baker Luo Sun Angela Howard John Buswell Damien Maurel Anastasiya Masharina Kai Johnsson Christopher J Noren Ming-Qun Xu Ivan R Corrêa

Chembiochem 2011 Sep 26;12(14):2217-26. Epub 2011 Jul 26.

New England Biolabs, Inc. 240 County Road, Ipswich, MA 01938, USA.

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September 2011

Measuring in vivo protein half-life.

Authors:

Karolina Bojkowska Francesca Santoni de Sio Isabelle Barde Sandra Offner Sonia Verp Christian Heinis Kai Johnsson Didier Trono

Chem Biol 2011 Jun;18(6):805-15

School of Life Sciences, Frontiers-in-Genetics National Program, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

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June 2011

A yeast-based screen reveals that sulfasalazine inhibits tetrahydrobiopterin biosynthesis.

Authors:

Christopher Chidley Hirohito Haruki Miriam Grønlund Pedersen Evelyne Muller Kai Johnsson

Nat Chem Biol 2011 Jun 17;7(6):375-83. Epub 2011 Apr 17.

Institute of Chemical Sciences and Engineering, Institute of Bioengineering, National Centre of Competence in Research Chemical Biology, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

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June 2011

Real-time measurements of protein dynamics using fluorescence activation-coupled protein labeling method.

Authors:

Toru Komatsu Kai Johnsson Hiroyuki Okuno Haruhiko Bito Takanari Inoue Tetsuo Nagano Yasuteru Urano

J Am Chem Soc 2011 May 7;133(17):6745-51. Epub 2011 Apr 7.

Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Tokyo, 113-0033, Japan.

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May 2011

Labelling cell structures and tracking cell lineage in zebrafish using SNAP-tag.

Authors:

Cláudia Campos Mako Kamiya Sambashiva Banala Kai Johnsson Marcos González-Gaitán

Dev Dyn 2011 Apr 28;240(4):820-7. Epub 2011 Feb 28.

Departement de Biochimie, Université de Genève, Genève, Switzerland.

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April 2011

Reflections on chemical biology.

Authors:

Kai Johnsson

ACS Chem Biol 2011 Jan;6(1):1-2

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January 2011

How to obtain labeled proteins and what to do with them.

Authors:

Marlon J Hinner Kai Johnsson

Curr Opin Biotechnol 2010 Dec 26;21(6):766-76. Epub 2010 Oct 26.

Institute of Chemical Sciences and Engineering, Laboratory of Protein Engineering, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

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December 2010

Benzothiazinones: prodrugs that covalently modify the decaprenylphosphoryl-β-D-ribose 2'-epimerase DprE1 of Mycobacterium tuberculosis.

Authors:

Claudia Trefzer Monica Rengifo-Gonzalez Marlon J Hinner Patricia Schneider Vadim Makarov Stewart T Cole Kai Johnsson

J Am Chem Soc 2010 Oct;132(39):13663-5

New Medicines for Tuberculosis Consortium, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

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October 2010

Using peptide loop insertion mutagenesis for the evolution of proteins.

Authors:

Christian Heinis Kai Johnsson

Methods Mol Biol 2010 ;634:217-32

Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de, Lausanne (EPFL), Lausanne, Switzerland.

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December 2010

Localizable and highly sensitive calcium indicator based on a BODIPY fluorophore.

Authors:

Mako Kamiya Kai Johnsson

Anal Chem 2010 Aug;82(15):6472-9

Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH 1015, Lausanne, Switzerland.

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August 2010

Fura-2FF-based calcium indicator for protein labeling.

Authors:

Agostina A Ruggiu Michael Bannwarth Kai Johnsson

Org Biomol Chem 2010 Aug 16;8(15):3398-401. Epub 2010 Jun 16.

Laboratory of Protein Engineering, Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL), CH-1015, Lausanne, Switzerland.

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August 2010