Publications by authors named "Kai Hu"

528 Publications

[Clinical analysis of oral and oropharyngeal cancer resection through oral approach].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2021 Aug;35(8):712-717

Department of Oral and Maxillofacial Surgery,the First Affiliated Hospital of Bengbu Medical College,Bengbu,233004,China.

To investigate the treatment effect of oral and oropharyngeal cancer resection through oral approach. Forty-eight patients who with oral and oropharyngeal cancer were admitted to the Department of Oral and Maxillofacial Surgery of the First Affiliated Hospital of Bengbu Medical College from January 2015 to January 2018, and all received preoperative chemotherapy, surgical treatment and postoperative radiotherapy. Among them, twenty-four patients who were treated with tumor resection through oral approach in the experimental group, the other twenty-four patients were treated with tumor resection by external approach in the control group. During the operation, both groups of patients were underwent selective neck lymph node dissection and free skin flap transplantation, and preventive radiotherapy were performed after the operation. The operation time, blood loss, and the positive rate of the wound around the wound and the undercut margin of the two groups were compared, and the survival rate of the skin flap was analyzed. The Kaplan-Meier method was used to calculate the survival rate after 3 years of regular follow-up after surgery, and the difference between the curves of the two surgical methods were compared by the Log-rank test, and the quality of life of patients in one year after operation was investigated and analyzed by Washington University students'quality questionnaire 4. The operation time and blood loss of the experimental group were less than the control group, but the difference was not statistically significant(>0.05). The positive rate of frozen margins in both groups was 0. The flap survival rate was 95.8% in the experimental group and 91.7% in the control group, there was no significant difference between the two groups(>0.05), the overall flap survival rate in the two groups was 93.8%. The survival rates of the experimental group were 91.7%, 83.3%, and 74.8% in the 1-, 2-, and 3-years after surgery, and 87.5%, 79.2%, and 75.0% in the control group, there was no statistically significant difference between the experimental group and the control group(>0.05). The 1-year, 2-year and 3-year overall survival rates of the two groups were 93.1%, 83.7% and 78.8% respectively. Compared with the control group, the scores of appearance, activity, recreation, swallowing, chewing, speech and mood in the experimental group were significantly higher(<0.05). Resection of oral cancer tumors through the oral approach with free skin flap repair is in line with the concept of minimally invasive surgery, which improves the quality of life of patients after surgery while ensuring the survival rate, and is worthy of clinical application and promotion.
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http://dx.doi.org/10.13201/j.issn.2096-7993.2021.08.009DOI Listing
August 2021

Preparation of Ti C T MXene based solid-phase microextraction coating for sensitive determination of polychlorinated biphenyls in environmental water samples.

J Sep Sci 2021 Jul 15. Epub 2021 Jul 15.

Center for Modern Analysis and Gene Sequencing, Zhengzhou University, Zhengzhou, P. R. China.

In this study, a new Ti C T -coated fiber was synthesized and utilized as coatings for solid-phase microextraction of seven polychlorinated biphenyls. The as-produced multilayered Ti C T MXene was characterized by X-ray diffractometer, thermos-gravimetric analysis, scanning electron microscopy, and energy dispersive spectroscopy. It is noteworthy that the Ti C T showed some attractive features including unique 2D layered structures, large surface area, good hydrophilicity, and rich active recognition sites, endowing it has a high affinity towards the target polychlorinated biphenyls. Subsequently, the affecting parameters on the extraction efficiency of polychlorinated biphenyls were optimized. Under the optimal conditions, a novel method for the analysis of polychlorinated biphenyls in water samples was proposed. The Ti C T -coated fiber-based solid-phase microextraction method showed good linearity (r  > 0.9928), high enrichment factors (268-442), low limits of detection (0.06-0.15 ng/L), and satisfactory repeatability (RSDs < 7.5%) for the polychlorinated biphenyls. The excellent method recoveries were in the range of 90.0-98.4, 92.0-98.2, and 92.0-98.0% for river water, lake water, and tap water samples, respectively. These results suggested that the proposed Ti C T -coated fiber-based method represents a promising alternative for the analysis of polychlorinated biphenyls.
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http://dx.doi.org/10.1002/jssc.202100247DOI Listing
July 2021

A new evaluation and prediction model of sound quality of high-speed permanent magnet motor based on genetic algorithm-radial basis function artificial neural network.

Sci Prog 2021 Jul-Sep;104(3):368504211031114

Nanjing Institute of Agricultural Mechanization, Ministry of Agriculture and Rural Affairs, Nanjing, China.

Sound quality (SQ) has become an important index to measure the competitiveness of motor products. To better evaluate and optimize SQ, a novelty SQ evaluation and prediction model of high-speed permanent magnet motor (HSPMM) with better accuracy is presented in this research. Six psychoacoustic parameters of A-weighted sound pressure level (ASPL), loudness, sharpness, roughness, fluctuation strength (FS), and perferred-frequency speech interference (PSIL) were adopted to objectively evaluate the SQ of HSPMM under multiple operating conditions and subjective evaluation was also conducted by the combination of semantic subdivision method and grade scoring method. The evaluation results show that the SQ is poor, which will have a certain impact on human psychology and physiology. The correlation between the objective evaluation parameters and the subjective scores is analyzed by coupling the subjective and objective evaluation results. The average error of multiple linear regression (MLR) model is 7.10%. It has good accuracy, but poor stability. In order to improve prediction accuracy, a new predicted model of radial basis function (RBF) artificial neural network was put forward based on genetic algorithm (GA) optimization. Compared with MLR, its average error rate is reduced by 3.16% and the standard deviation is reduced by 1.841. In addition, the weight of each objective parameter was analyzed. The new predicted model has a better accuracy. It can evaluate and optimize the SQ exactly. The research methods and conclusions of this paper can be extended to the evaluation, prediction, and optimization of SQ of other motors.
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http://dx.doi.org/10.1177/00368504211031114DOI Listing
July 2021

Improvement of Resistive Switching Performance in Sulfur-Doped HfOx-Based RRAM.

Materials (Basel) 2021 Jun 16;14(12). Epub 2021 Jun 16.

Tianjin Key Laboratory of Film Electronic and Communication Devices, School of Electrical and Electronic Engineering, Tianjin University of Technology, Tianjin 300384, China.

In order to improve the electrical performance of resistive random access memory (RRAM), sulfur (S)-doping technology for HfOx-based RRAM is systematically investigated in this paper. HfOx films with different S-doping contents are achieved by atmospheric pressure chemical vapor deposition (APCVD) under a series of preparation temperatures. The effect of S on crystallinity, surface topography, element composition of HfOx thin films and resistive switching (RS) performance of HfOx-based devices are discussed. Compared with an undoped device, the V/V of the S-doped device with optimal S content (~1.66 At.%) is reduced, and the compliance current (Icc) is limited from 1 mA to 100 μA. Moreover, it also has high uniformity of resistance and voltage, stable endurance, good retention characteristics, fast response speed (SET 6.25 μs/RESET 7.50 μs) and low energy consumption (SET 9.08 nJ/RESET 6.72 nJ). Based on X-ray photoelectron spectroscopy (XPS) data and fitting of the high/low resistance state (HRS/LRS) conduction behavior, a switching mechanism is considered to explain the formation and rupture of conductive filaments (CFs) composed of oxygen vacancies in undoped and S-doped HfOx-based devices. Doping by sulfur is proposed to introduce the appropriate concentration oxygen vacancies into HfOx film and suppress the random formation of CFs in HfOx-based device, and thus improve the performance of the TiN/HfOx/ITO device.
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http://dx.doi.org/10.3390/ma14123330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233777PMC
June 2021

CRDet: Improving Signet Ring Cell Detection by Reinforcing the Classification Branch.

J Comput Biol 2021 Jul 29;28(7):732-743. Epub 2021 Jun 29.

School of Computer Science, Xiangtan University, Xiangtan, China.

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http://dx.doi.org/10.1089/cmb.2020.0555DOI Listing
July 2021

Associations of mixture of air pollutants with estimated 10-year atherosclerotic cardiovascular disease risk modified by socio-economic status: The Henan Rural Cohort Study.

Sci Total Environ 2021 Jun 18;793:148542. Epub 2021 Jun 18.

Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, Henan, PR China. Electronic address:

Background: Low socio-economic status (SES) and exposure to single-air pollutant relate to increased prevalent atherosclerotic cardiovascular diseases (ASCVD), however, interactive effect between SES and exposure to single- or multiple-air pollutants on high 10-year ASCVD risk remains unclear.

Methods: A total of 31,162 individuals were derived from the Henan Rural Cohort Study. Concentrations of air pollutants (particulate matter with an aerodynamic diameter ≤ 1.0 μm (PM), ≤2.5 μm (PM) or ≤10 μm (PM), nitrogen dioxide (NO)) were assessed using a spatiotemporal model based on satellites data. Independent and joint associations of SES, single- and multiple- air pollutants with high 10-year ASCVD risk were evaluated using logistic regression models, quantile g-computation and structural equation models. The interactive effects of SES and exposure to single- or multiple air pollutants on high 10-year ASCVD risk were visualized by using Interaction plots.

Results: Exposure to single air pollutant (PM, PM, PM or NO) related to increased high 10-year ASCVD risk among individuals with low education level or personal average monthly income, compared to the ones with high education level or personal average monthly income. Furthermore, similar results of exposure to mixture of air pollutants with high 10-year ASCVD risk were observed. Positive interactive effects between low SES and exposure to high single air pollutant or the mixture of air pollutants on high 10-year ASCVD risk were observed.

Conclusion: Positive association of low SES with high 10-year ASCVD risk was amplified by exposure to high levels of single air pollutant or a mixture of air pollutants, implying that individuals with low SES may more susceptible to air pollution-related adverse health effect.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148542DOI Listing
June 2021

Deep supervised learning using self-adaptive auxiliary loss for COVID-19 diagnosis from imbalanced CT images.

Neurocomputing 2021 Oct 7;458:232-245. Epub 2021 Jun 7.

Key Laboratory of Intelligent Computing and Information Processing of Ministry of Education, Xiangtan University, Xiangtan 411105, China.

The outbreak and rapid spread of coronavirus disease 2019 (COVID-19) has had a huge impact on the lives and safety of people around the world. Chest CT is considered an effective tool for the diagnosis and follow-up of COVID-19. For faster examination, automatic COVID-19 diagnostic techniques using deep learning on CT images have received increasing attention. However, the number and category of existing datasets for COVID-19 diagnosis that can be used for training are limited, and the number of initial COVID-19 samples is much smaller than the normal's, which leads to the problem of class imbalance. It makes the classification algorithms difficult to learn the discriminative boundaries since the data of some classes are rich while others are scarce. Therefore, training robust deep neural networks with imbalanced data is a fundamental challenging but important task in the diagnosis of COVID-19. In this paper, we create a challenging clinical dataset (named COVID19-Diag) with category diversity and propose a novel imbalanced data classification method using deep supervised learning with a self-adaptive auxiliary loss (DSN-SAAL) for COVID-19 diagnosis. The loss function considers both the effects of data overlap between CT slices and possible noisy labels in clinical datasets on a multi-scale, deep supervised network framework by integrating the effective number of samples and a weighting regularization item. The learning process jointly and automatically optimizes all parameters over the deep supervised network, making our model generally applicable to a wide range of datasets. Extensive experiments are conducted on COVID19-Diag and three public COVID-19 diagnosis datasets. The results show that our DSN-SAAL outperforms the state-of-the-art methods and is effective for the diagnosis of COVID-19 in varying degrees of data imbalance.
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http://dx.doi.org/10.1016/j.neucom.2021.06.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180474PMC
October 2021

The α2AR/Caveolin-1/p38MAPK/NF-κB axis explains dexmedetomidine protection against lung injury following intestinal ischaemia-reperfusion.

J Cell Mol Med 2021 Jun 10. Epub 2021 Jun 10.

Department of Anesthesiology, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Intestinal ischaemia-reperfusion (I/R) injury can result in acute lung injury due to ischaemia and hypoxia. Dexmedetomidine (Dex), a highly selective alpha2-noradrenergic receptor (α2AR) agonist used in anaesthesia, is reported to regulate inflammation in organs. This study aimed to investigate the role and mechanism of Dex in lung injury caused by intestinal I/R. After establishing a rat model of intestinal I/R, we measured the wet-to-dry specific gravity of rat lungs upon treatments with Dex, SB239063 and the α2AR antagonist Atipamezole. Moreover, injury scoring and histopathological studies of lung tissues were performed, followed by ELISA detection on tumour necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 expression. Correlation of Caveolin-1 (Cav-1) protein expression with p38, p-p38, p-p65 and p65 in rat lung tissues was analysed, and the degree of cell apoptosis in lung tissues after intestinal I/R injury was detected by TUNEL assay. The lung injury induced by intestinal I/R was a dynamic process. Moreover, Dex had protective effects against lung injury by mediating the expression of Cal-1 and α -AR. Specifically, Dex promoted Cav-1 expression via α -AR activation and mitigated intestinal I/R-induced lung injury, even in the presence of Atipamezole. The protective effect of Dex on intestinal I/R-induced lung injury was also closely related to α -AR/p38 mitogen-activated protein kinases/nuclear factor-kappaB (MAPK/NF-κB) pathway. Dex can alleviate pulmonary inflammation after in intestinal I/R by promoting Cav-1 to inhibit the activation of p38 and NF-κB. In conclusion, Dex can reduce pulmonary inflammatory response even after receiving threats from both intestinal I/R injury and Atipamezole.
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http://dx.doi.org/10.1111/jcmm.16614DOI Listing
June 2021

High expression predicts poor prognosis in patients with Head and Neck Squamous Cell Carcinomas.

J Cancer 2021 5;12(13):3887-3899. Epub 2021 May 5.

Department of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

Overexpression of the membrane protein SEC61 translocon gamma subunit (SEC61G) has been observed in a variety of cancers; however, its role in head and neck squamous cell carcinomas (HNSCC) is unknown. This study aimed to elucidate the relationship between and HNSCC based on data from The Cancer Genome Atlas (TCGA) database. Data for HNSCC patients were collected from TCGA and the expression level of was compared between paired HNSCC and normal tissues using the Wilcoxon rank-sum test. The relationship between clinicopathologic features and expression was also analyzed using the Wilcoxon rank-sum test and logistic regression. Receiver operating characteristic (ROC) curves were generated to evaluate the value of as a binary classifier using the area under the curve (AUC value). The association of clinicopathologic characteristics with prognosis in HNSCC patients was assessed using Cox regression and the Kaplan-Meier methods. A nomogram, based on Cox multivariate analysis, was used to predict the impact of on prognosis. Functional enrichment analysis was performed to determine the hallmark pathways associated with differentially expressed genes in HNSCC patients exhibiting high and low expression. The expression of was significantly elevated in HNSCC tissues compared to normal tissues (P < 0.001). The high expression of was significantly correlated with the T stage, M stage, clinical stage, mutation status, mutation status, primary therapy outcome, and cervical lymph node dissection (all P < 0.05). Meanwhile, ROC curves suggested the significant diagnostic ability of for HNSCC (AUC = 0.923). Kaplan-Meier survival analysis showed that patients with HNSCC characterized by high expression had a poorer prognosis than patients with low expression (hazard ratio = 1.95, 95% confidence interval 1.48-2.56, P < 0.001). Univariate and multivariate analyses revealed that was independently associated with overall survival (P = 0.027). Functional annotations indicated that SEC61G is involved in pathways related to translation and regulation of SLITs/ROBOs expression, SRP-dependent co-translational protein targeting to the membrane, nonsense-mediated decay, oxidative phosphorylation, and Parkinson's disease. plays a vital role in HNSCC progression and prognosis; it may, therefore, serve as an effective biomarker for the prediction of patient survival.
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http://dx.doi.org/10.7150/jca.51467DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176234PMC
May 2021

Loss of m6A demethylase ALKBH5 promotes post-ischemic angiogenesis via post-transcriptional stabilization of WNT5A.

Clin Transl Med 2021 May;11(5):e402

Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai, China.

Background: Post-ischemic angiogenesis is critical for blood flow recovery and ischemic tissue repair. N6-methyladenosine (m6A) plays essential roles in numerous biological processes. However, the impact and connected mechanism of m6A on post-ischemic angiogenesis are not fully understood.

Methods: AlkB homolog 5 (ALKBH5) was screened out among several methyltransferases and demethylases involved in dynamic m6A regulation. Cardiac microvascular endothelial cells (CMECs) angiogenesis and WNT family member 5A (WNT5A) stability were analyzed upon ALKBH5 overexpression with adenovirus or knockdown with small interfering RNAs in vitro. The blood flow recovery, capillary, and small artery densities were evaluated in adeno-associated virus (AAV)-ALKBH5 overexpression or ALKBH5 knockout (KO) mice in a hind-limb ischemia model. The same experiments were conducted to explore the translational value of transient silencing of ALKBH5 with adenovirus.

Results: ALKBH5 was significantly upregulated in hypoxic CMECs and led to a global decrease of m6A level. ALKBH5 overexpression further reduced m6A level in normoxic and hypoxic CMECs, impaired proliferation, migration, and tube formation only in hypoxic CMECs. Conversely, ALKBH5 knockdown preserved m6A levels and promoted angiogenic phenotypes in hypoxic but not in normoxic CMECs. Mechanistically, ALKBH5 regulated WNT5A expression through post-transcriptional mRNA modulation in an m6A-dependent manner, which decreased its stability and subsequently impeded angiogenesis in hypoxic CMECs. Furthermore, ALKBH5 overexpression hindered blood flow recovery and reduced CD31 and alpha-smooth muscle actin expression in hind-limb ischemia mice. As expected, ALKBH5-KO mice exhibited improved blood flow recovery, increased capillary, and small artery densities after hind-limb ischemia, and similar beneficial effects were observed in mice with transient adenoviral ALKBH5 gene silencing.

Conclusion: We demonstrate that ALKBH5 is a negative regulator of post-ischemic angiogenesis via post-transcriptional modulation and destabilization of WNT5A mRNA in an m6A-dependent manner. Targeting ALKBH5 may be a potential therapeutic option for ischemic diseases, including peripheral artery disease.
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http://dx.doi.org/10.1002/ctm2.402DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087997PMC
May 2021

Photo-initiated enhanced antibacterial therapy using a non-covalent functionalized graphene oxide nanoplatform.

Dalton Trans 2021 Jun;50(24):8404-8412

School of Materials and Chemical Engineering, Zhongyuan University of Technology, Zhengzhou 450007, P.R. China.

This study describes a novel antibacterial phototherapeutic platform for highly efficient healing of bacteria-infected wounds. It is based on the photodynamic and physical actions of a zinc tetraaminophthalocyanine-modified graphene oxide nanocomposite produced via non-covalent functionalization. The nanocomposite is positively charged and can easily capture negatively charged bacteria via electrostatic interactions. The antibacterial action is two-fold: (1) reactive oxygen species are produced by the phthalocyanine photosensitizer after short-term exposure to 680 nm light and (2) the graphene oxide can physically cut bacterial cell membranes. These enhanced activities can kill Gram-positive and Gram-negative bacteria at very low dosages. An ultrastructural examination indicates that this nanocomposite causes enormous damage to bacterial morphology and leakage of intracellular substances that lead to bacterial death. A rat wound model is used to demonstrate that the proposed phototherapeutic platform has low cytotoxicity and can promote rapid healing in bacteria-infected wounds. These results suggest that the integration of different antibacterial methods into a single nanotherapeutic platform is a promising strategy for anti-infective treatment.
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http://dx.doi.org/10.1039/d1dt00642hDOI Listing
June 2021

Analysis of dose monitoring for external exposure to radiology in hospital workers from 2010 to 2018.

Am J Transl Res 2021 15;13(4):3357-3362. Epub 2021 Apr 15.

Department of Infection Prevention and Control, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, National Clinical Research Center for Oral Diseases, Beijing Key Laboratory of Digital Stomatology Beijing, China.

Objective: To investigate the individual level of radiation exposure in hospital workers from 2010 to 2018.

Methods: Oral radiology workers in our hospital including medical imaging technicians and radiation therapists from 2010 to 2018 were selected as the subjects of investigation. The oral radiological workers were monitored quarterly according to the level of external exposure via individual dose monitoring standards. The monitoring data were aggregated, analyzed and evaluated.

Results: A total of 531 hospital radiology workers were monitored from 2010 to 2018. The rate of effective monitoring per year for medical imaging technicians and radiation therapists was 97.35% and 97.47%, respectively. The average collective effective dose was 8.511 mSv, and annual effective dose per capita was 0.148 mSv. The highest collective effective dose was in 2017, while the highest annual effective dose per capita was in 2010. The annual effective dose per capita for medical imaging technicians was lower than that for radiation therapists. The abnormal rate of personal doses of radiation therapists was higher than that for medical imaging technicians. The collective effective dose changes in the two types of radiation workers were monitored from 2010 to 2018, showing an increased trend. The fluctuations of annual effective dosing per capita monitored from 2010 to 2018 in radiation therapists was more significant than that in medical imaging technicians.

Conclusions: Oral radiation workers monitored were all far below the dose limit of 20 mSv, which indicated that the working environment of oral radiation workers in our hospital was safe with good radiation condition and protection.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129226PMC
April 2021

GSDMD-Mediated Cardiomyocyte Pyroptosis Promotes Myocardial I/R Injury.

Circ Res 2021 Jul 21;129(3):383-396. Epub 2021 May 21.

Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China (H.S., Y.G., Z.D., J.Y., X.L., S.Z., L.M., F.Z., K.H., A.S., J.G.).

[Figure: see text].
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http://dx.doi.org/10.1161/CIRCRESAHA.120.318629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8291144PMC
July 2021

Heterologous vaccination regimens with self-amplifying RNA and adenoviral COVID vaccines induce robust immune responses in mice.

Nat Commun 2021 05 17;12(1):2893. Epub 2021 May 17.

Nuffield Department of Medicine, The Jenner Institute, University of Oxford, Oxford, UK.

Several vaccines have demonstrated efficacy against SARS-CoV-2 mediated disease, yet there is limited data on the immune response induced by heterologous vaccination regimens using alternate vaccine modalities. Here, we present a detailed description of the immune response, in mice, following vaccination with a self-amplifying RNA (saRNA) vaccine and an adenoviral vectored vaccine (ChAdOx1 nCoV-19/AZD1222) against SARS-CoV-2. We demonstrate that antibody responses are higher in two-dose heterologous vaccination regimens than single-dose regimens. Neutralising titres after heterologous prime-boost were at least comparable or higher than the titres measured after homologous prime boost vaccination with viral vectors. Importantly, the cellular immune response after a heterologous regimen is dominated by cytotoxic T cells and Th1 CD4 T cells, which is superior to the response induced in homologous vaccination regimens in mice. These results underpin the need for clinical trials to investigate the immunogenicity of heterologous regimens with alternate vaccine technologies.
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http://dx.doi.org/10.1038/s41467-021-23173-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129084PMC
May 2021

[Study on the correlation between the content of bone morphogenetic protein 2 in demineralized bone matrix and its osteogenic activity and ].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2021 May;35(5):620-626

Shanghai Yapeng Biotechnology Co., Ltd, Shanghai, 201201, P.R.China.

Objective: To investigate the correlation between the content of bone morphogenetic protein 2 (BMP-2) in demineralized bone matrix (DBM) and its osteogenic activity and , in order to choose a simple and convenient method to evaluate the osteogenic activity of DBM.

Methods: The left mid-femoral tissues of 9 donors were taken, and DBMs (S1-S9) were prepared by dynamic decalcification process, and inactivated DBM (control group) was prepared at the same time. Protease inhibitor method, collagenase method, guanidine hydrochloride/ethylene diamine tetraacetic acid (EDTA) method, and RIPA lysate method were used to extract BMP-2 in S1-S9 and inactivated DBMs. The BMP-2 content was measured and the differences between DBMs were compared. Then the S1-S9 and inactivated DBMs were co-cultured with mouse embryonic osteoblasts MC3T3-E1, respectively. The cell proliferation was detected by MTT method and fluorescence staining, and alkaline phosphatase (ALP) activity was detected at the same time. Thirty BALB/c male nude mice were divided into 10 groups, namely S1-S9 DBM groups (S1-S9 groups) and inactivated DBM group (control group), with 3 mice in each group. Muscle pockets of the middle thighs were prepared on both hindlimbs of mice in each group, and implanted corresponding DBM materials. At 4 weeks after operation, the samples were taken for HE staining observation and semi-quantitative evaluation, and the new bone formation score was calculated.

Results: The BMP-2 content of DBM derived from different donor bones was distinct. The BMP-2 content obtained by different extraction methods for DBM prepared from the same donor bone was also different, and the extraction efficiency of the guanidine hydrochloride/EDTA method was the highest. cell experiments, MTT test displayed that cell proliferations and ALP activity were significantly higher in S4 and S6 groups than in other groups at each time point after co-cultivation ( <0.05). Moreover, the cell proliferation of S4 group was the most significant at 7 days ( <0.05); fluorescence staining demonstrated that the osteoblasts of each group was in good condition, but the osteoblasts of S1, S2, S3, S4, and S6 groups were significantly more than other groups. ectopic osteogenesis experiments, the cartilage and new bone formation could be seen in the bone graft area of S1-S6 groups at 4 weeks after operation, and with the increase of BMP-2 content, the more new bone formation induced by the material, the higher the score of new bone formation of the material ( <0.05). Among them, S4 and S6 groups contained a large number of chondrocytes and osteoblasts in the osteogenesis area.

Conclusion: The osteogenic activity of DBM can be evaluated through BMP-2 quantitative detection combined with osteoblast proliferation and differentiation experiments.
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http://dx.doi.org/10.7507/1002-1892.202012006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175199PMC
May 2021

The impact of government-enterprise collusion on environmental pollution in China.

Authors:
Kai Hu Daqian Shi

J Environ Manage 2021 Aug 11;292:112744. Epub 2021 May 11.

School of Economics, Zhongnan University of Economics and Law, Wuhan, 430073, China. Electronic address:

Pollution emissions in China are associated with the relationship between local governments and enterprises, especially in those cities with government-enterprise collusion (GEC). We evaluate the causal relationships between GEC and SO emissions at the enterprise level, by adopting the Propensity Score Matching-Difference in Difference method from a comprehensive environmental database. The empirical results show that, compared with those in the cities without collusion, SO emissions of enterprises in the colluded cities increase by 11.3% (95% Confidence Interval (CI): 0.041-0.186). These GEC effects are more substantial in the cities whose regional officials work with longer terms, in the foreign-owned or small-scale enterprises, and the labour-intensive industries. The findings suggest the existing environment and personnel management policies in China should be adjusted for more sustainable development.
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http://dx.doi.org/10.1016/j.jenvman.2021.112744DOI Listing
August 2021

Characterization of the mitochondrial genome of (Macquart, 1838) (Diptera: Stratiomyidae: Pachygastrinae).

Authors:
Kai Hu Zaihua Yang

Mitochondrial DNA B Resour 2021 Apr 15;6(4):1378-1379. Epub 2021 Apr 15.

Guizhou Academy of Forestry, Guiyang, PR China.

We have assembled and annotated the mitochondrial genome of (Macquart, 1838) (a species of soldier fly) in this study. It is 15,495 bp in length, including 13 protein-coding genes (PCGs), two ribosomal genes (s), 22 transfer genes (s), and a large non-coding control region (length: 704 bp). The nucleotide composition of whole mitochondrial genome biases toward A and T (75.5%). Most PCGs use ATN as initiation codon, except for which starts with CGA. All PCGs end with common termination codon TAA/G. Phylogenetic analyses based on the nucleotide sequence data supported the monophyly of Stratiomyidae and the sister relationship between Pachygastrinae and the clade (Nemotelinae + (Hermetiinae + Sarginae)).
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http://dx.doi.org/10.1080/23802359.2021.1910081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8078917PMC
April 2021

Population Pharmacokinetics of Lithium in Young Pediatric Patients With Intellectual Disability.

Front Pharmacol 2021 15;12:650298. Epub 2021 Apr 15.

Henan Key Laboratory of Child Brain Injury and Henan Pediatric Clinical Research Center, Institute of Neuroscience and Third Affiliated Hospital and of Zhengzhou University, Zhengzhou, China.

Lithium is a well-established treatment for bipolar disorders and has been shown to be neuroprotective, and thus low doses might be useful for the treatment of childhood brain injury and neurological sequelae. However, pharmacokinetic (PK) data in children are limited. This study was to investigate the PKs after oral administration of low-dose lithium carbonate in young children with intellectual disability. Fifty-two children with intellectual disability aged 4-10 years old were enrolled. A series of blood samples were collected after a single-dose administration of lithium carbonate. The serum lithium concentration was measured using a validated ion chromatography assay, and the PK concentration data were modeled using a nonlinear mixed effect model in the NONMEM program. The lithium concentration over time was adequately described by a two-compartment disposition, with a transient absorption and first-order elimination process. The inclusion of body weight as an allometric factor significantly improved the model fit, but age and gender were not associated with the PKs of lithium. The clearance, central volume, inter-compartmental clearance, and peripheral volume estimates from the final population PK model were 0.98 L/h, 13.1 L, 0.84 L/h, and 8.2 L for children with a body weight of 20 kg. The model evaluation suggested that there is no obvious discrepancy between the observations and predictions in the proposed model. A visual predictive check demonstrated the good predictive performance of the final model. The lithium PK properties in young children were similar to those in older children and adults. The proposed model can be used for further PK/PD analysis to optimize the dosage regimen of lithium in children.
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http://dx.doi.org/10.3389/fphar.2021.650298DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082156PMC
April 2021

Impact of diastolic dysfunction on outcome in heart failure patients with mid-range or reduced ejection fraction.

ESC Heart Fail 2021 Aug 1;8(4):2802-2815. Epub 2021 May 1.

Department of Internal Medicine I, University Hospital Würzburg, Oberdürrbacher Str. 6, Würzburg, 97080, Germany.

Aims: The role of diastolic dysfunction (DD) in prognostic evaluation in heart failure (HF) patients with impaired systolic function remains unclear. We investigated the impact of echocardiography-defined DD on survival in HF patients with mid-range (HFmrEF, EF 41-49%) and reduced ejection fraction (HFrEF, EF < 40%).

Methods And Results: A total of 2018 consecutive hospitalized HF patients were retrospectively included and divided in two groups based on baseline EF: HFmrEF group (n = 951, aged 69 ± 13 years, 74.2% male) and HFrEF group (n = 1067, aged 68 ± 13 years, 76.3% male). Clinical data were collected and analysed. All patients completed ≥1 year clinical follow-up. The primary endpoint was defined as all-cause death (including heart transplantation) and cardiovascular (CV)-related death. All-cause mortality (30.8% vs. 24.9%, P = 0.003) and CV mortality (19.1% vs. 13.5%, P = 0.001) were significantly higher in the HFrEF group than the HFmrEF group during follow-up [median 24 (13-36) months]. All-cause mortality increased in proportion to DD severity (mild, moderate, and severe) in either HFmrEF (17.1%, 25.4%, and 37.0%, P < 0.001) or HFrEF (18.9%, 30.3%, and 39.2%, P < 0.001) patients. The risk of all-cause mortality [hazard ratio (HR) = 1.347, P = 0.015] and CV mortality (HR = 1.508, P = 0.007) was significantly higher in HFrEF patients with severe DD compared with non-severe DD after adjustment for identified clinical and echocardiographic covariates. For HFmrEF patients, severe DD was independently associated with increased all-cause mortality (HR = 1.358, P = 0.046) but not with CV mortality (HR = 1.155, P = 0.469).

Conclusions: Echocardiography-defined severe DD is independently associated with increased all-cause mortality in patients with HFmrEF and HFrEF.
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http://dx.doi.org/10.1002/ehf2.13352DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8318417PMC
August 2021

Aging Characteristics of Transformer Oil-Impregnated Insulation Paper Based on Trap Parameters.

Polymers (Basel) 2021 Apr 22;13(9). Epub 2021 Apr 22.

Institute of Advanced Electrical Materials, Qingdao University of Science and Technology, Qingdao 266042, China.

Oil-impregnated insulation paper is an important part of transformers; its performance seriously affects the life of power equipment. It is of significance to study the aging characteristics and mechanism of oil-impregnated insulation paper under thermal stress for transformer status detection and evaluation. In the work, the accelerated thermal aging was carried out at 120 °C, and DP1490, DP787, and DP311 samples were selected to represent the new, mid-aging, and late-aging status of the transformer, respectively. The space charge distribution within the specimens was measured by the pulsed electro-acoustic (PEA) method and the trap parameters were extracted based on the measurement curves. Further, the aging mechanism was studied by molecular simulation technology. A typical molecular chain defect model was constructed to study the motion of cellulose molecules under thermal stress. The experimental results show that the corresponding trap energy levels are 0.54 eV, 0.73 eV, and 0.92 eV for the new specimen, the mid-aging specimen, and the late aging specimen, respectively. The simulation results show that the trapped energy at the beginning of aging is mainly determined by the loss of H atoms. The changes in trap energy in the middle stage of aging are mainly caused by the absence of some C atoms, and the trap energy level at the end of aging is mainly caused by the breakage of chemical bonds. This study is of great significance to reveal the aging mechanism of oil-impregnated insulation paper and the modification of insulation paper.
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http://dx.doi.org/10.3390/polym13091364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122526PMC
April 2021

CCL19 and CCL28 Assist Herpes Simplex Virus 2 Glycoprotein D To Induce Protective Systemic Immunity against Genital Viral Challenge.

mSphere 2021 04 28;6(2). Epub 2021 Apr 28.

State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China

Potent systemic immunity is important for recalled mucosal immune responses, but in the defense against mucosal viral infections, it usually remains low at mucosal sites. Based on our previous findings that enhanced immune responses can be achieved by immunization with an immunogen in combination with a molecular adjuvant, here we designed chemokine-antigen (Ag) fusion constructs (CCL19- or CCL28-herpes simplex virus 2 glycoprotein D [HSV-2 gD]). After intramuscular (i.m.) immunization with different DNA vaccines in a prime and boost strategy, BALB/c mice were challenged with a lethal dose of HSV-2 through the genital tract. Ag-specific immune responses and chemokine receptor-specific lymphocytes were analyzed to determine the effects of CCL19 and CCL28 in strengthening humoral and cellular immunity. Both CCL19 and CCL28 were efficient in inducing long-lasting HSV-2 gD-specific systemic immunity. Compared to CCL19, less CCL28 was required to elicit HSV-2 gD-specific serum IgA responses, Th1- and Th2-like responses of immunoglobulin (Ig) subclasses and cytokines, and CCR3 T cell enrichment (>8.5-fold) in spleens. These findings together demonstrate that CCL28 tends to assist an immunogen to induce more potently protective immunity than CCL19. This work provides information for the application potential of a promising vaccination strategy against mucosal infections caused by HSV-2 and other sexually transmitted viruses. An effective HSV-2 vaccine should induce antigen (Ag)-specific immune responses against viral mucosal infection. This study reveals that chemokine CCL19 or CCL28 enhanced HSV-2 glycoprotein D ectodomain (gD-306aa)-induced immune responses against vaginal virus challenge. In addition to eliciting robust humoral immune responses, the chemokine-Ag fusion construct also induced Th1- and Th2-like immune responses characterized by the secretion of multiple Ig subclasses and cytokines that were able to be recalled after HSV-2 challenge, while CCL28 appeared to be more effective than CCL19 in promoting gD-elicited immune responses as well as the migration of T cells to secondary lymph tissues. Of importance, both CCL19 and CCL28 significantly facilitated gD to induce protective mucosal immune responses in the genital tract. The above-described findings together highlight the potential of CCL19 or CCL28 in combination with gD as a vaccination strategy to control HSV-2 infection.
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http://dx.doi.org/10.1128/mSphere.00058-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092132PMC
April 2021

Hypertrophic preconditioning cardioprotection after myocardial ischaemia/reperfusion injury involves ALDH2-dependent metabolism modulation.

Redox Biol 2021 07 31;43:101960. Epub 2021 Mar 31.

Department of Cardiology, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China; NHC Key Laboratory of Viral Heart Diseases, Shanghai, China; Key Laboratory of Viral Heart Diseases, Chinese Academy of Medical Sciences, Shanghai, China. Electronic address:

Brief episodes of ischaemia and reperfusion render the heart resistant to subsequent prolonged ischaemic insult, termed ischaemic preconditioning. Here, we hypothesized that transient non-ischaemic stress by hypertrophic stimulation would induce endogenous cardioprotective signalling and enhance cardiac resistance to subsequent ischaemic damage. Transient transverse aortic constriction (TAC) or Ang-Ⅱ treatment was performed for 3-7 days in male mice and then withdrawn for several days by either aortic debanding or discontinuing Ang-Ⅱ treatment, followed by subsequent exposure to regional myocardial ischaemia by in situ coronary artery ligation. Following ischaemia/reperfusion (I/R) injury, myocardial infarct size and apoptosis were markedly reduced and contractile function was significantly improved in the TAC preconditioning group compared with that in the control group. Similar results were observed in mice receiving Ang-Ⅱ infusion. Mechanistically, TAC preconditioning enhanced ALDH2 activity, promoted AMPK activation and improved mitochondrial energy metabolism by increasing myocardial OXPHOS complex expression, elevating the mitochondrial ATP content and improving viable myocardium glucose uptake. Moreover, TAC preconditioning significantly mitigated I/R-induced myocardial iNOS/gp91 activation, inhibited endoplasmic reticulum stress and ameliorated mitochondrial impairment. Using a pharmacological approach to inhibit AMPK signalling in the presence or absence of preconditioning, we demonstrated AMPK-dependent protective mechanisms of TAC preconditioning against I/R injury. Furthermore, treatment with adenovirus-encoded ALDH2 partially emulated the actions of hypertrophic preconditioning, as evidenced by improved mitochondrial metabolism, inhibited oxidative stress-induced mitochondrial damage and attenuated cell death through an AMPK-dependent mechanism, whereas genetic ablation of ALDH2 abrogated the aforementioned actions of TAC preconditioning. The present study demonstrates that preconditioning with hypertrophic stress protects the heart from I/R injury via mechanisms that improve mitochondrial metabolism, reduce oxidative/nitrative stress and inhibit apoptosis. ALDH2 is obligatorily required for the development of cardiac hypertrophic preconditioning and acts as the mediator of this process.
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http://dx.doi.org/10.1016/j.redox.2021.101960DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099646PMC
July 2021

Enzyme-Initiated Assembly of an Extracellular-Like Two-Dimensional Nanonetwork as a Method to Detect Procancerous Activity.

ACS Sens 2021 05 28;6(5):1815-1822. Epub 2021 Apr 28.

Department of Laboratory Medicine, the Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing 210003, China.

Extracellular matrix (ECM) enzymes such as lysyl oxidase (LOX) provide a new possibility to contain the invasive progress of cancer. Unlike conventional enzymes, the activity of ECM enzymes is not simply the conversion of the substrate to the product; the amount of enzymes such as matrix metalloproteinases in the ECM changes the structural integrity and morphology of the ECM. These are all important aspects that must be monitored in a spatiotemporally coupled fashion to fully understand their procancerous effect. To achieve this goal, a new molecular probe is developed, which, unlike antibodies or aptamers, can interact with the target enzyme in a more interactive way: the probe can withdraw the metal ion cofactor of the enzyme and modulate its catalytic ability. This can lead to self-propagated cross-linking of the probes to form a network not dissimilar to the collagen and elastin network of the ECM, formed through LOX activity. Thus, the biosensing process itself is a biomimetic of what may occur in vivo in the ECM, and three distinct types of signal readouts can be simultaneously recorded on the sensing surface to provide a fuller picture of ECM enzyme activity, not achievable with traditional designs. Using this method, a parallel between the detected signal and the progress of colorectal cancer can be observed. These results may point to prospective application of this method in evaluating ECM-related tumor invasiveness in the future.
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http://dx.doi.org/10.1021/acssensors.0c02651DOI Listing
May 2021

Proteome profiling reveals the efficacy and targets of sophocarpine against asthma.

Int Immunopharmacol 2021 Jul 13;96:107348. Epub 2021 Apr 13.

Shaanxi Academy of Traditional Chinese Medicine (Shaanxi Traditional Chinese Medicine Hospital), Xi'an 710003, PR China. Electronic address:

Sophocarpine (SPC) as a quinolizidine alkaloid displays powerful effects on inflammatory diseases through regulating multiple targets. Asthma is a complex heterogeneous and inflammatory disease with an increasing incidence worldwide. Here we established a mice asthma model and investigated the effect of SPC. Mice induced by ovalbumin (OVA) exhibits exacerbated Th1/Th2 immune imbalance and allergic lung inflammation. SPC treatment regulated Th1/Th2 cytokines production (IL-4, IL-5 and INF-γ) in BALF, reduced IgE level in serum, inhibited inflammatory cell infiltration, and improved the lung tissue pathology. Proteomic results showed that 5064 proteins in lung tissue were detected and among them 223 preliminary therapeutic targets of SPC were selected. Subsequently, excluding non-human genes, 109 targets with established crystal structures were harvested. Meanwhile, the molecular docking results showed that the binding energy of 87 targets with SPC was varied from -9.72 kcal/mol to 227.16 kcal/mol. Further, SPC suppressed arrb2, anxa1, myd88 and sphk1 expression and activated p-stat1. All of the five targets based on the screened results of proteomics and molecular docking are critical in allergic asthma. Thus, our data revealed that SPC alleviated bronchial asthma via targeting multi-targets.
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http://dx.doi.org/10.1016/j.intimp.2020.107348DOI Listing
July 2021

[Development and Clinical Application of Portable Electronic Endoscope].

Zhongguo Yi Liao Qi Xie Za Zhi 2021 Apr;45(2):153-158

Zhejiang Center for Medical Device Evaluation, Hangzhou, 311121.

Electronic endoscope is one of the crucial tools for minimally invasive diagnosis and treatment. Along with the continuous development of clinical applications, conventional electronic endoscope has not been able to meet all the clinical requirements. In recent years, with the rapid development of the material, sensor and electronic technologies, electronic endoscope has attracted many researchers and companies' attention. Nowadays, because of being suitable for some special mobile medical scenes like field operations, natural calamities and hospital first aids, various portable electronic endoscopes have been developed and applied in clinic. The present study aims to give a review on portable electronic endoscopes development and clinical applications.
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http://dx.doi.org/10.3969/j.issn.1671-7104.2021.02.007DOI Listing
April 2021

Bipartite Network of Interest (BNOI): Extending Co-Word Network with Interest of Researchers Using Sensor Data and Corresponding Applications as an Example.

Sensors (Basel) 2021 Mar 1;21(5). Epub 2021 Mar 1.

Key Laboratory of Advanced Process Control for Light Industry, Ministry of Education, Jiangnan University, Wuxi 214122, China.

Traditional co-word networks do not discriminate keywords of researcher interest from general keywords. Co-word networks are therefore often too general to provide knowledge if interest to domain experts. Inspired by the recent work that uses an automatic method to identify the questions of interest to researchers like "problems" and "solutions", we try to answer a similar question "what sensors can be used for what kind of applications", which is great interest in sensor- related fields. By generalizing the specific questions as "questions of interest", we built a knowledge network considering researcher interest, called bipartite network of interest (BNOI). Different from a co-word approaches using accurate keywords from a list, BNOI uses classification models to find possible entities of interest. A total of nine feature extraction methods including N-grams, Word2Vec, BERT, etc. were used to extract features to train the classification models, including naïve Bayes (NB), support vector machines (SVM) and logistic regression (LR). In addition, a multi-feature fusion strategy and a voting principle (VP) method are applied to assemble the capability of the features and the classification models. Using the abstract text data of 350 remote sensing articles, features are extracted and the models trained. The experiment results show that after removing the biased words and using the ten-fold cross-validation method, the F-measure of "sensors" and "applications" are 93.2% and 85.5%, respectively. It is thus demonstrated that researcher questions of interest can be better answered by the constructed BNOI based on classification results, comparedwith the traditional co-word network approach.
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http://dx.doi.org/10.3390/s21051668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7957500PMC
March 2021

Mitogenome of Yang & Yang, 2010 (Diptera: Stratiomyidae) and its phylogenetic implications.

Authors:
Kai Hu Zaihua Yang

Mitochondrial DNA B Resour 2021 Mar 17;6(3):914-915. Epub 2021 Mar 17.

Guizhou Academy of Forestry, Guiyang, P. R. China.

The complete mitogenome of Yang & Yang, 2010 (Stratiomyidae: Pachygastrinae) successfully sequenced. The mitogenome of is a circular DNA molecule of 16,069 bp in length with 72% AT content, consisting of 13 PCGs, two rRNAs, 22 tRNAs, and a large control region. The gene order is consistent with other dipteran mitogenomes. The phylogenetic tree was reconstructed using maximum likelihood analysis, and the topology revealed that Pachygastrinae is not a monophyletic group.
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http://dx.doi.org/10.1080/23802359.2021.1886887DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971276PMC
March 2021

Association of Small Intestinal Bacterial Overgrowth With Heart Failure and Its Prediction for Short-Term Outcomes.

J Am Heart Assoc 2021 Apr 17;10(7):e015292. Epub 2021 Mar 17.

Department of Cardiology Shanghai Institute of Cardiovascular DiseasesZhongshan HospitalFudan University Shanghai China.

Background Small intestinal bacterial overgrowth (SIBO) is a common pathological condition of intestinal microbiota. The prevalence of SIBO and its prognostic value in patients with heart failure (HF) are unknown. Methods and Results A total of 287 patients tested for SIBO using lactulose hydrogen-methane breath test were evaluated. At least 1 of the following criteria fulfilled was SIBO positive: patients with fasting hydrogen level ≥20 parts per million (ppm) or a ≥20 ppm rise in hydrogen by 90 minutes were diagnosed with SIBO (H) positive; and patients with methane levels ≥10 ppm at any test point were diagnosed with SIBO (CH) positive. The association between SIBO and the composite of cardiovascular death and HF rehospitalization was investigated. In 287 consecutive patients with HF, 128 (45%) were positive for SIBO. Our result showed SIBO increased the risk of HF rehospitalization in patients with HF with reduced ejection fraction (<0.001), and the risk of cardiovascular death in patients with HF with preserved EF (=0.011). SIBO was an independent risk factor of primary end point in patients with HF (hazard ratio [HR], 2.13; 95% CI; 1.26-3.58; =0.005). In addition, SIBO (CH) showed a prognostic value on adverse outcomes (HR, 2.35; 95% CI, 1.38-4.02; <0.001), whereas the association between SIBO (H) and outcomes was not statistically significant. Conclusions There was high prevalence of SIBO in patients with HF, and SIBO was independently associated with poor outcomes. Proactive treatment for SIBO may provide extra benefit for patients with HF.
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http://dx.doi.org/10.1161/JAHA.119.015292DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8174348PMC
April 2021

miRNAs derived from circulating small extracellular vesicles as diagnostic biomarkers for nasopharyngeal carcinoma.

Cancer Sci 2021 Jun 5;112(6):2393-2404. Epub 2021 Apr 5.

Department of Radiation Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

The microRNAs (miRNAs) in circulating small extracellular vesicles (sEVs) have been suggested as potential biomarkers in cancer diagnosis. This study was designed to evaluate the circulating sEV-derived miRNAs as biomarkers for the diagnosis of nasopharyngeal carcinoma (NPC). We compared the miRNA profiles in plasma-derived sEVs between 16 patients with NPC and 5 healthy controls (HCs). A distinct set of miRNAs that were differentially expressed between patients with NPC and HCs was determined by means of integrative bioinformatics approaches. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathway analysis revealed that the target genes of the differentially expressed miRNAs (DEMs) were mainly involved in cancer-associated signaling pathways. Seven representative DEMs were selected and further validated in an additional 60 patients with NPC and 40 HCs using quantitative reverse-transcription PCR analysis (qRT-PCR). Receiver operating characteristic (ROC) curve analysis was used to assess the accuracy of the sEV-miRNA-based model for diagnosis. The 3 miRNA-based model, comprising miR-134-5p, miR-205-5p, and miR-409-3p, showed good discriminating power with an area under the curve (AUC) value of 0.88 in the training set and 0.91 in the validation set. Furthermore, the diagnostic model had an excellent classification ability to distinguish patients with NPC at different clinical stages or Epstein-Barr virus infection status from HCs. In conclusion, our findings indicated that sEV-derived miRNA levels were altered in the plasma of patients with NPC in comparison with those in HCs. The model based on the 3 sEV-derived miRNAs could potentially act as an alternative or complementary approach for diagnosing NPC.
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http://dx.doi.org/10.1111/cas.14883DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177774PMC
June 2021
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