Publications by authors named "Kai Fan"

218 Publications

Assessment of Human Exposure to Five Mycotoxins in China by Biomonitoring Approach.

Toxins (Basel) 2021 Oct 28;13(11). Epub 2021 Oct 28.

Key Laboratory of Protected Horticultural Technology, Institute for Agro-Food Standards and Testing Technology, Academy of Agricultural Sciences, Shanghai 201403, China.

This biomonitoring study was conducted to investigate the concentration levels of five mycotoxins in urine samples from 269 healthy volunteers living in the Yangtze River Delta, China. Alternariol (AOH), alternariol monomethyl ether (AME), tenuazonic acid (TeA) and tentoxin (TEN) were detected in 38.3%, 48.7%, 63.9% and 23.4% of urine samples with the concentrations ranging from 0.057 to 45.8 ng/mL, 0.020 to 0.802 ng/mL, 0.050 to 80.6 ng/mL and 0.021 to 0.939 ng/mL, respectively. Altenuene (ALT) was not detected in any urine sample. Based on the urinary concentrations, the probable daily intake (PDI) values of mycotoxins were calculated, and 100%, 99.2-100%, 0.372% and 1.12% of participants exceeded the threshold of toxicological concern (TTC) values for AOH, AME, TeA and TEN, respectively. This study revealed high potential health risks related to the contaminations of major mycotoxins in China and highlighted the necessity for more toxicological studies to provide better basis for further comprehensive risk assessments.
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http://dx.doi.org/10.3390/toxins13110762DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625692PMC
October 2021

Ultrasound and its combined application in the improvement of microbial and physicochemical quality of fruits and vegetables: A review.

Ultrason Sonochem 2021 Dec 17;80:105838. Epub 2021 Nov 17.

College of Life Science, Yangtze University, Jingzhou, Hubei 434025, China.

The eating safety and high quality of fruits and vegetables have always been concerned by consumers, so require a safe, non-toxic, environment-friendly technology for their preservation. The application of ultrasound is a potential technology in the preservation of fruits and vegetables. This paper describes the ultrasound mechanism for inactivating microorganisms, with the cavitation phenomena of ultrasound being considered as a main effect. Effect of ultrasound on microorganisms of fruits and vegetables was discussed. Ultrasound alone and its combined treatments can be an effective method to inactivate the spoilage and pathogenic microorganisms on the surface of fruit and vegetables. Effect of ultrasound on physicochemical quality of fruits and vegetables was reviewed. Ultrasound and its combined treatments reduced mass loss, decreased color change, maintained firmness, enhanced and inhibited enzyme activity as well as preserving nutritional components such as total phenolic, total flavonoids, anthocyanin, and ascorbic acid.
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http://dx.doi.org/10.1016/j.ultsonch.2021.105838DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8605411PMC
December 2021

Image Quality Evaluation of Sanda Sports Video Based on BP Neural Network Perception.

Authors:
Kai Fan Xiaoye Gu

Comput Intell Neurosci 2021 27;2021:5904400. Epub 2021 Oct 27.

Physical Education Department, University of International Business and Economics, Beijing, China.

In the special sports camera, there are subframes. A lens is composed of multiple frames. It will be unclear if a frame is cut out. The definition of video screenshots lies in the quality of video. To get clear screenshots, we need to find clear video. The purpose of this paper is to analyze and evaluate the quality of sports video images. Through the semantic analysis and program design of video using computer language, the video images are matched with the data model constructed by research, and the real-time analysis of sports video images is formed, so as to achieve the real-time analysis effect of sports techniques and tactics. In view of the defects of rough image segmentation and high spatial distortion rate in current sports video image evaluation methods, this paper proposes a sports video image evaluation method based on BP neural network perception. The results show that the optimized algorithm can overcome the slow convergence of weights of traditional algorithm and the oscillation in error convergence of variable step size algorithm. The optimized algorithm will significantly reduce the learning error of neural network and the overall error of network quality classification and greatly improve the accuracy of evaluation. Sanda motion video image quality evaluation method based on BP (back propagation) neural network perception has high spatial accuracy, good noise iteration performance, and low spatial distortion rate, so it can accurately evaluate Sanda motion video image quality.
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http://dx.doi.org/10.1155/2021/5904400DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8566064PMC
November 2021

Correlation among Metabolic Changes in Tea Plant (L.) Shoots, Green Tea Quality and the Application of Cow Manure to Tea Plantation Soils.

Molecules 2021 Oct 13;26(20). Epub 2021 Oct 13.

Tea Research Institute, Qingdao Agricultural University, Qingdao 266109, China.

Traditionally, the supplement of organic manure in tea plantations has been a common approach to improving soil fertility and promoting terroir compounds, as manifested by the coordinated increase in yield and quality for the resulting teas. However, information regarding the effect of organic manure in the metabolome of tea plants is still inadequate. The metabolite profiles of tea shoots applied with cow manure, urea or no fertilizer were studied using gas chromatography-mass spectrometry (GC-MS). In total, 73 metabolites were detected, and the modulated metabolites included mainly amino acids, organic acids and fatty acids. In particular, glutamine, quinic acid and proline accumulated more in tea shoots in soils treated with cow manure, but octadecanoic acid, hexadecanoic acid and eicosanoic acid were drastically reduced. Pearson correlation analysis indicated that organic acids and amino acids in tea shoots were the two major metabolite groups among the three treatments. The analysis of metabolic pathways demonstrated that the cow manure treatment significantly changed the enrichment of pathways related to amino acids, sugars and fatty acids. Sensory evaluation showed that the quality of green teas was higher when the plants used to make the tea were grown in soil treated with cow manure rather than urea during spring and late summer. The results indicated that the application of cow manure in soils changed the metabolic characteristics of tea shoots and improved the qualities of the resulting teas.
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http://dx.doi.org/10.3390/molecules26206180DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538533PMC
October 2021

Prediction of Drought-Induced Components and Evaluation of Drought Damage of Tea Plants Based on Hyperspectral Imaging.

Front Plant Sci 2021 19;12:695102. Epub 2021 Aug 19.

Tea Research Institute, Qingdao Agricultural University, Qingdao, China.

Effective evaluation of physiological and biochemical indexes and drought degree of tea plant is an important technology to determine the drought resistance ability of tea plants. At present, the traditional detection method of tea drought stress is mainly based on physiological and biochemical detection, which is not only destructive to tea plants, but also time-consuming and laborious. In this study, through simulating drought treatment of tea plant, hyperspectral camera was used to obtain spectral data of tea leaves, and three machine learning models, namely, support vector machine (SVM), random forest (RF), and partial least-squares (PLS) regression, were used to model malondialdehyde (MDA), electrolyte leakage (EL), maximum efficiency of photosystem II (), soluble saccharide (SS), and drought damage degree (DDD) of tea leaves. The results showed that the competitive adaptive reweighted sampling (CARS)-PLS model of MDA had the best effect among the four physiological and biochemical indexes (Rcal = 0.96, Rp = 0.92, RPD = 3.51). Uninformative variable elimination (UVE)-SVM model was the best in DDD (Rcal = 0.97, Rp = 0.95, RPD = 4.28). Therefore, through the establishment of machine learning model using hyperspectral imaging technology, we can monitor the drought degree of tea seedlings under drought stress. This method is not only non-destructive, but also fast and accurate, which is expected to be widely used in tea garden water regime monitoring.
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http://dx.doi.org/10.3389/fpls.2021.695102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8417055PMC
August 2021

Ezrin regulates synovial angiogenesis in rheumatoid arthritis through YAP and Akt signalling.

J Cell Mol Med 2021 10 29;25(19):9378-9389. Epub 2021 Aug 29.

Department of Joint and Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, China.

This study aimed to investigate the role and regulatory mechanisms of Ezrin in synovial vessels in rheumatoid arthritis (RA). Synovial tissues were obtained from people with osteoarthritis people and patients with RA patients. We also used an antigen-induced arthritis (AIA) mice model by using Freund's adjuvant injections. Ezrin expression was analysed by immunofluorescence and immunohistochemical staining in synovial vessels of patients with RA and AIA mice. We investigated the role of Ezrin on vascular endothelial cells and its regulatory mechanism in vivo and in vitro by adenoviral transfection technology. Our results suggest a role for the Ezrin protein in proliferation, migration and angiogenesis of vascular endothelial cells in RA. We also demonstrate that Ezrin plays an important role in vascular endothelial cell migration and tube formation through regulation of the Hippo-yes-associated protein 1 (YAP) pathway. YAP, as a key protein, can further regulate the activity of PI3K/Akt signalling pathway in vascular endothelial cells. In AIA mice experiments, we observed that the inhibition of Ezrin or of its downstream YAP pathway can affect synovial angiogenesis and may lead to progression of RA. In conclusion, Ezrin plays an important role in angiogenesis in the RA synovium by regulating YAP nuclear translocation and interacting with the PI3K/Akt signalling pathway.
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http://dx.doi.org/10.1111/jcmm.16877DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8500952PMC
October 2021

Inkjet-Printed Highly Conductive Poly(3,4-ethylenedioxythiophene): Poly(styrenesulfonate) Electrode for Organic Light-Emitting Diodes.

Micromachines (Basel) 2021 Jul 28;12(8). Epub 2021 Jul 28.

State Key Laboratory of Organic Electronics and Information Displays, Institute of Advanced Materials (IAM), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.

Recently, inkjet printing technology has attracted much attention due to the advantages of drop-on-demand deposition, low-cost and large-area production for organic light-emitting diode (OLED) displays. However, there are still some problems in industrial production and practical application, such as the complexity of ink modulation, high-quality films with homogeneous morphology, and the re-dissolution phenomenon at interfaces. In this work, a printable poly(3,4-ethylenedioxythiophene): poly(styrenesulfonate) (PEDOT:PSS) ink is developed and obtains an adjustable viscosity. Finally, a patterned PEDOT:PSS electrode is fabricated by inkjet printing, and achieves a high conductivity of 1213 S/cm, a transparency of 86.8% and a uniform morphology without coffee-ring effect. Furthermore, the vacuum-evaporated and solution-processed OLEDs are fabricated based on this electrode and demonstrate a current efficiency of 61 cd/A, which is comparable to that of the indium tin oxide counterpart. This work confirms the feasibility of inkjet printing technology to prepare patterned electrodes and expects that it can be used to fabricate highly efficient optoelectronic devices.
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http://dx.doi.org/10.3390/mi12080889DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8398279PMC
July 2021

Research Progress on the Mechanism of the Acupuncture Regulating Neuro-Endocrine-Immune Network System.

Vet Sci 2021 Jul 30;8(8). Epub 2021 Jul 30.

College of Veterinary Medicine, China Agricultural University, No. 2, Yuanmingyuan West Road, Haidian District, Beijing 100193, China.

As one of the conventional treatment methods, acupuncture is an indispensable component of Traditional Chinese Medicine. Currently, acupuncture has been partly accepted throughout the world, but the mechanism of acupuncture is still unclear. Since the theory of the neuro-endocrine-immune network was put forward, new insights have been brought into the understanding of the mechanism of acupuncture. Studies have proven that acupuncture is a mechanical stimulus that can activate local cell functions and neuroreceptors. It also regulates the release of related biomolecules (peptide hormones, lipid hormones, neuromodulators and neurotransmitters, and other small and large biomolecules) in the microenvironment, where they can affect each other and further activate the neuroendocrine-immune network to achieve holistic regulation. Recently, growing efforts have been made in the research on the mechanism of acupuncture. Some researchers have transitioned from studying the mechanism of acupuncture as a single linear pathway to using systems approaches, including metabolomics, genomics, proteomics and biological pathway analysis. This review summarizes the research progress on the neuro-endocrine-immune network related mechanism of acupuncture and discusses its current challenges and future directions.
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http://dx.doi.org/10.3390/vetsci8080149DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8402722PMC
July 2021

Using UAV image data to monitor the effects of different nitrogen application rates on tea quality.

J Sci Food Agric 2021 Aug 23. Epub 2021 Aug 23.

Tea Research Institute, Qingdao Agricultural University, Qingdao, China.

Background: Accurate and efficient evaluation of the effect of nitrogen application rate on tea quality is of great significance for nitrogen management in a tea garden. However, previous methods were all through soil or leaf sampling, using biochemical methods for laboratory testing. These methods are not only less one-time detection samples, but also time-consuming, laborious and inefficient. Therefore, the development of fast, efficient and non-destructive diagnostic methods is an important goal in this field.

Results: We obtained spectral information on the tea canopy using a multispectral camera carried by an unmanned aerial vehicle (UAV), and extracted the average DN value of the experimental plot by environmental visual imagery (ENVI); we finally obtained 28 spectral parameters. By analyzing the correlation between spectral parameters and ground parameters measured synchronously, five spectral parameters with high correlation were selected. Finally, the prediction models of tea nitrogen, polyphenol and amino acid content were established by using support vector machine (SVM), partial least squares and backpropagation neural network. Through modeling comparison and coefficient verification, the results show that the ground parameters measured in the laboratory were in good agreement with the results estimated by the model. The SVM model had the best performance in predicting nitrogen and tea polyphenol content, with R  = 0.7583 and 0.7533, root mean square error of prediction (RMSEP) = 0.4086 and 0.3392, and normalized RMSEP (NRMSEP) = 1.23 and 1.28, respectively. The partial least squares regression model had the best performance in predicting amino acid content, with R  = 0.7597, RMSEP = 0.1176 and NRMSEP = 4.10.

Conclusion: The results show that the model based on UAV image data and machine learning algorithm can effectively detect the main biochemical components of the tea plant, which provides an important basis for tea garden management. © 2021 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.11489DOI Listing
August 2021

Untargeted metabolomics of purple and orange-fleshed sweet potatoes reveals a large structural diversity of anthocyanins and flavonoids.

Sci Rep 2021 08 12;11(1):16408. Epub 2021 Aug 12.

Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, USA.

Anthocyanins are economically valuable phytochemicals of significant relevance to human health. Industrially extracted from multiple fruit and vegetable sources, anthocyanin yield and profiles can vary between sources and growing conditions. In this study, we focused on three purple-fleshed and one orange-fleshed cultivars of sweet potato-a warm-weather, nutritious crop of substantial interest to growers in northern, cooler latitudes-to determine the yield and diversity of anthocyanins and flavonoids. Acidified ethanol extraction of lyophilized roots yielded ~ 800 mg average anthocyanins/100 g dry weight from all three cultivars. UHPLC-DAD-Orbitrap analysis of sweet potato extracts identified 18 high-confidence, mostly acylated peonidin and cyanidin derivatives contributing to > 90% of the total anthocyanin signal. Further assessment of the untargeted Liquid Chromatography-Tandem Mass Spectrometry data using deep learning and molecular networking identified over 350 flavonoid peaks with variable distributions in different sweet potato cultivars. These results provide a novel insight into anthocyanin content of purple-fleshed sweet potatoes grown in the northern latitudes, and reveal the large structural diversity of anthocyanins and flavonoids in this popular crop.
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http://dx.doi.org/10.1038/s41598-021-95901-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361111PMC
August 2021

Anti-fouling and protein separation of [email protected] filtration membrane with in-situ grown MnO nanorods.

Chemosphere 2022 Jan 3;286(Pt 2):131756. Epub 2021 Aug 3.

Key Laboratory of Interfacial Physics and Technology, Chinese Academy of Sciences, Shanghai, 201800, China; Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory (SSRF, ZJLab), Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201204, China. Electronic address:

MnO nanorods with controllable scale were grown in the PVDF-g-PMAA modified membrane to form [email protected] MnO membrane through the in situ redox reaction of KMnO solution, which is confirmed by scanning electron microscopy (SEM) and X-ray energy-dispersion spectroscopy (EDX). The pore size of the membrane decreased with the increase of KMnO solution concentration. The thermodynamic stability and the hydrophilicity of the membrane were also enhanced by the MnO nanorods. The water flux, bovine serum albumin (BSA)/Lysozyme protein solution flux and rejection, flux recovery, etc. showed effective improvement of the anti-fouling performance of the [email protected] MnO membrane. More importantly, it can effectively separate BSA from lysozyme, which provided a potential application in the field of biology, food, and other industrial fields for the requirement of separation and purification.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131756DOI Listing
January 2022

The Egr-1/miR-15a-5p/GPX4 axis regulates ferroptosis in acute myocardial infarction.

Eur J Pharmacol 2021 Oct 31;909:174403. Epub 2021 Jul 31.

Department of Pharmacology, Harbin Medical University-Daqing, No 39, Xin Yang Road, Daqing, Heilongjiang, 163319, China. Electronic address:

Acute myocardial infarction (AMI) is a type of cardiovascular diseases that severely threatens human being, but the mechanisms have not been thoroughly clarified. Here, we detected that microRNA-15a-5p (miR-15a-5p) was up-regulated in AMI. Knockdown of miR-15a-5p reduced cell mortality in hypoxic-treated myocardial cells. In addition, we determined that glutathione peroxidase4 (GPX4) was the direct target of miR-15a-5p by luciferase reporter assay. Over-expression of miR-15a-5p strengthened ferroptosis, then aggravated myocardial cell hypoxia injury. Mechanistically, silencing transcription factor early growth response-1 (Egr-1) inhibited the level of miR-15a-5p, increased the protein expression of GPX4, accompanied by reduced ferroptosis and alleviated myocardial injury. In summary, these results provide a novel signaling pathway during the progression of acute myocardial infarction, namely Egr-1/miR-15a-5p/GPX4/ferroptosis.
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http://dx.doi.org/10.1016/j.ejphar.2021.174403DOI Listing
October 2021

Analysis of the characteristics of outpatient and emergency diseases in the department of otolaryngology during the "COVID-19" pandemic.

Sci Prog 2021 Jul-Sep;104(3):368504211036319

Department of Otolaryngology, Head and Neck Surgery, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.

The pandemic of "Corona Virus Disease 2019" (COVID-19) has changed the lives of people. There have been changes in common outpatient and emergency cases in otolaryngology, so an analysis of data pertaining to this was completed. This study is to evaluate the impact of viral infection disease in otolaryngological common disease. This study uses the data of common diseases in the outpatient and emergency department during the "COVID-19" pandemic (from February to April 2020) and the same period in the past 3 years from the Department of Otolaryngology. During the "COVID-19" period compared with the same period last year, the ranking of cases by diseases has changed. Diseases such as chronic pharyngitis, allergic rhinitis, sudden deafness, and tinnitus increased, meanwhile acute pharyngitis and acute laryngopharyngitis decreased ( < 0.05). The viral infection has impacted the mental behaviors of people, therefore mental-related disease cases of the department of Otolaryngology have increased indirectly. This study provides real data to illustrate mental-related diseases. It also provides experience and shows the importance of keeping and maintaining mental health.
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http://dx.doi.org/10.1177/00368504211036319DOI Listing
August 2021

Fulvic acid enhances drought resistance in tea plants by regulating the starch and sucrose metabolism and certain secondary metabolism.

J Proteomics 2021 09 21;247:104337. Epub 2021 Jul 21.

Tea Research Institute, Shandong Academy of Agricultural Sciences, Rizhao, Shandong, China; Tea Research Institute, Qingdao Agricultural University, Qingdao, Shandong, China. Electronic address:

The aim of this work was to gain insight into the molecular mechanisms underlying the effect of fulvic acid on drought-exposed tea plants. We performed proteomic analysis of fulvic acid-treated tea leaves from the target plants using tandem mass tag quantitative labeling technology and compared the results with those of a previous transcriptomic analysis. We identified 48 and 611 differentially abundant proteins in the leaves of tea plants treated with fulvic acid compared with the control under mild and severe drought, respectively. Comparative analysis showed that, under severe drought, 55 genes had similar expression patterns at the transcriptome and proteome levels, such as PAL, GBE, GBSS and bAS. Bioinformatic analysis revealed that those genes were mainly related to the starch and sucrose metabolism, phenylpropanoid biosynthesis and triterpenoid biosynthesis. SIGNIFICANCE: This study broadens the understanding of the molecular mechanisms underlying the improved drought resistance seen in tea plants in the presence of fulvic acid and provides a basis for further research on the genomics of drought tolerance in these plants. In addition, these findings could be used to develop new guidance strategies for improved drought management systems in tea plantation.
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http://dx.doi.org/10.1016/j.jprot.2021.104337DOI Listing
September 2021

Genome-wide identification and molecular evolution analysis of the heat shock transcription factor (HSF) gene family in four diploid and two allopolyploid Gossypium species.

Genomics 2021 Sep 9;113(5):3112-3127. Epub 2021 Jul 9.

Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; Key Laboratory of Crop Ecology and Molecular Physiology (Fujian Agriculture and Forestry University), Fujian Province University, Fuzhou 35002, PR China. Electronic address:

Heat shock transcription factors (HSFs) can regulate plant development and stress response. The comprehensive evolutionary history of the HSF family remains elusive in cotton. In this study, each cotton species had 78 members in Gossypium barbadense and Gossypium hirsutum. The diploid species had 39 GaHSFs in Gossypium arboreum, 31 GrHSFs in Gossypium raimondii, 34 GtHSFs in Gossypium turneri, and 34 GlHSFs in Gossypium longicalyx. The HSF family in cotton can be classified into three subfamilies, with seven groups in subfamily A and five groups in subfamily B. Different groups exhibited distinct gene proportions, conserved motifs, gene structures, expansion rates, gene loss rates, and cis-regulatory elements. The paleohexaploidization event led to the expansion of the HSF family in cotton, and the gene duplication events in six Gossypium species were inherited from their common ancestor. The HSF family in diploid species had a divergent evolutionary history, whereas two cultivated tetraploids presented a highly conserved evolution of the HSF family. The HSF members in At and Dt subgenomes of the cultivated tetraploids showed a different evolution from their corresponding diploid donors. Some HSF members were regarded as key candidates for regulating cotton development and stress response. This study provided the comprehensive information on the evolutionary history of the HSF family in cotton.
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http://dx.doi.org/10.1016/j.ygeno.2021.07.008DOI Listing
September 2021

Cathepsin C aggravates neuroinflammation via promoting production of CCL2 and CXCL2 in glial cells and neurons in a cryogenic brain lesion.

Neurochem Int 2021 09 23;148:105107. Epub 2021 Jun 23.

Department of Anatomy, College of Basic Medical Sciences, Dalian Medical University, Dalian, Liaoning, China; National-Local Joint Engineering Research Center for Drug-Research and Development (R&D) of Neurodegenerative Diseases, Dalian Medical University, Dalian, Liaoning, China. Electronic address:

Objective: Chemokines regulate infiltration of immune cells to brain in inflammation. Cathepsin C (CatC), a lysosomal protease, has been found to participate in neuroinflammation. However, how CatC affects chemokines expression in neuroinflammation triggered by traumatic brain injury (TBI) remains unclear. Here, we investigated the effects of CatC on chemokines and neuroinflammation in TBI.

Methods: The present study used CatC knockdown (KD) and overexpression (OE) mice to generate cryogenic brain lesion model and determined effects of CatC on expression of chemokines CCL2, CCL5 and CXCL2 and infiltration of immune cells in acute and chronic phases of the lesion. Further, cellular sources of various chemokines were demonstrated in vitro. Values were compared with wild type (WT) mice.

Results: The results found that 6 h after lesion, CatC expression,IL-1β and TNF-α mRNA and protein expression were strongly induced in the lesions; CCL2 and CXCL2 mRNA and protein expression were increased in CatC OE mice, while decreased in CatC KD mice. On the 3rd day after lesion, macrophages and neutrophils were mainly infiltrated to the lesions. Simultaneously, Iba-1+ cells in CatC OE mice were increased, while MPO + cells in CatC KD mice were decreased. In contrast, on the 28th day after lesion, a few lymphocytes were infiltrated surrounding new blood vessels. CatC OE mice showed larger volumes of scar areas, higher expression of CCL2,CXCL2,IL-1β,TNF-α,IL-6 and iNOS, as well as stronger GFAP+ and Iba-1+ signals, while CatC KD mice had reversed effects. No significant differences of CCL5 expression were found in various genotype mice. Further, in vitro study demonstrated CatC-induced expression of CCL2 were mainly derived from microglia and neurons, while CXCL2 derived from microglia and astrocytes.

Conclusion: Our data indicate that CatC aggravates neuroinflammation via promoting production of CCL2 and CXCL2 in glial cells and neurons in a cryogenic brain lesion, providing potential cellular and molecular targets for future intervention of TBI and other neuroinflammatory diseases.
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http://dx.doi.org/10.1016/j.neuint.2021.105107DOI Listing
September 2021

Risk factors and predictive model of adrenocortical insufficiency in patients with traumatic brain injury.

World J Emerg Med 2021 ;12(3):179-184

Department of Emergency, the First Hospital of Shanxi Medical University, Taiyuan 030001, China.

Background: Neuroendocrine dysfunction after traumatic brain injury (TBI) has received increased attention due to its impact on the recovery of neural function. The purpose of this study is to investigate the incidence and risk factors of adrenocortical insufficiency (AI) after TBI to reveal independent predictors and build a prediction model of AI after TBI.

Methods: Enrolled patients were grouped into the AI and non-AI groups. Fourteen preset impact factors were recorded. Patients were regrouped according to each impact factor as a categorical variable. Univariate and multiple logistic regression analyses were performed to screen the related independent risk factors of AI after TBI and develop the predictive model.

Results: A total of 108 patients were recruited, of whom 34 (31.5%) patients had AI. Nine factors (age, Glasgow Coma Scale [GCS] score on admission, mean arterial pressure [MAP], urinary volume, serum sodium level, cerebral hernia, frontal lobe contusion, diffuse axonal injury [DAI], and skull base fracture) were probably related to AI after TBI. Three factors (urinary volume [ ], serum sodium level [ ], and DAI [ ]) were independent variables, based on which a prediction model was developed (logit = -3.552+2.583 +2.235 +2.269 ).

Conclusions: The incidence of AI after TBI is high. Factors such as age, GCS score, MAP, urinary volume, serum sodium level, cerebral hernia, frontal lobe contusion, DAI, and skull base fracture are probably related to AI after TBI. Urinary volume, serum sodium level, and DAI are the independent predictors of AI after TBI.
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http://dx.doi.org/10.5847/wjem.j.1920-8642.2021.03.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8188278PMC
January 2021

A novel Danshensu/tetramethylpyrazine protects against Myocardial Ischemia Reperfusion Injury in rats.

Int J Med Sci 2021 13;18(12):2716-2724. Epub 2021 May 13.

Department of Pharmacology, Harbin Medical University-Daqing, Daqing, 163319, China.

A new Danshensu/tetramethylpyrazine derivative (ADTM) with cardio-protection effects such as antioxidant, arterial relaxation, pro-angiogenesis and antiplatelet activities. Platelet activating factor receptor (PAFR) plays a key role in myocardial ischemia reperfusion (MIR) injury. This study aims to investigate the protective role of ADTM in MIR injury and clarify the potential role of PAFR. We measured the effects of ADTM on MIR injury in rats and hypoxia re-oxygenation (HR) injury in neonatal rat ventricular myocytes (NRVMs) . The results show that ADTM can significantly improve the IR-induced decline in heart function as increasing EF and FS, and restore the decreased cardiac hemodynamic parameters (LVSP, ± dp/dt max) and increased the level of LVEDP, decrease the infarct size of damaged myocardium and lactate dehydrogenase (LDH) activity in serum. Additionally, ADTM inhibits cardiomyocytes apoptosis, caspase-3 activity, and inflammatory response as well as down-regulates the MIR-induced IL-1β and TNFα production. Next, PAFR expression was significantly down-regulated in cardiomyocytes of MIR model and after treated with ADTM compare to IR group. At the same time, ADTM and PAFR small interfering RNA (siRNA) could inhibit cardiomyocytes apoptosis and inflammation during HR, while PAF presents the opposite effect. Furthermore, the above effects of PAF in HR induced cardiomyocytes were reversed by co-treatment of ADTM. Our findings demonstrate for the first time that ADTM protects against MIR injury through inhibition of PAFR signaling, which provides a new treatment for MIR.
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http://dx.doi.org/10.7150/ijms.59411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176181PMC
May 2021

Ambient ozone pollution at a coal chemical industry city in the border of Loess Plateau and Mu Us Desert: characteristics, sensitivity analysis and control strategies.

PeerJ 2021 27;9:e11322. Epub 2021 Apr 27.

College of Resource and Environment Engineering, Guizhou University, Guiyang, China.

In this study, ambient ozone (O) pollution characteristics and sensitivity analysis were carried out in Yulin, a city in the central area of the Loess Plateau during 2017 to 2019 summer. O concentrations increased for 2017 to 2019. Correlation and statistics analysis indicated high temperature (T > 25 °C, low relative humidity (RH < 60%), and low wind speed (WS < 3 m/s) were favorable for O formation and accumulation, and the O pollution days (MDA8 O > 160 µg/m) were predominantly observed when the wind was traveling from the easterly and southerly. O concentration in urban area of Yulin was higher than that in background. The pollution air masses from Fenwei Plain increase the level and duration of O pollution. In order to clarify the formation mechanism and source of O, online measurements of volatile organic compounds (VOCs) were conducted from 7 July to 10 August in 2019. The average of VOCs concentration was 26 ± 12 ppbv, and large amounts of alkenes followed by aromatics, characteristic pollutants of the coal chemical industry, were detected in the ambient air. To further measure the sensitivity, the observation-based model (OBM) simulation was conducted. Empirical Kinetic Modeling Approach (EKMA) plot and relative incremental reactivity (RIR) value indicated Yulin located on VOCs-limited regime. That implied a slight decrease of NO may increase O concentration. When the emission reduction ratio of anthropogenic VOCs/NO higher than 1:1, the O will decrease. O control strategies analysis shows that the O targets of 5% and 10% O concentration reductions are achievable through precursor control, but more effort is needed to reach the 30% and 40% reduction control targets.
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http://dx.doi.org/10.7717/peerj.11322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086580PMC
April 2021

Necrotizing fasciitis of the jaw, neck and mediastinum caused by Klebsiella oxytoca and Streptococcus constellatus: a case report.

Ann Palliat Med 2021 Jul 11;10(7):8431-8436. Epub 2021 May 11.

Department of Otolaryngology, Head and Neck Surgery, Tongji Hospital of Tongji University, Tongji University School of Medicine, Shanghai, China.

Necrotizing fasciitis (NF) is a rare, fulminant, lethal soft-tissue infection result in fascial necrosis, it is rarer in the head and neck area. Infection caused by Klebsiella oxytoca is much less common. Therefore, we reported a case of NF in the maxillofacial region, neck and upper mediastinum caused by Klebsiella oxytoca as the main cause recently treated in our department. The patient is a middle-aged male with a 10-year history of diabetes with unstable insulin control. The main symptoms were pain on left side with dysphagia and fever and the situation was getting worse. The patient had limited ability to have mouth open and had hyperemia, swelling on the left pharynx, maxillofacial area, and upper left neck and skin tenderness, and all symptoms were getting worse quick. The CT mainly found out that left oropharyngeal wall, parotid gland area, bilateral submaxilla, left neck, and superior mediastinum are swelling with gas. The blood test result: leukocytes count 16.64×109/L, neutrophils percentage 85.8%; C-Pr 320 mg/L; urinary routine: urine glucose (+++++), ketone bodies (+++++); fasting glucose metabolism: glucose 21.33 mmol/L, glycosylated albumin 47.67%. Three incisions of facial and neck were performed to drain pus. Result of bacteria culture: Klebsiella oxytoca and Streptococcus constellatus During treatment of DKA and reduce patient glucose level, we also treated patient with neck and trachea incisions to drain pus and cleaned daily wound area and used different antibiotics according to the bacteria culture and CT results. And finally, the patient was cured and discharged from hospital. This case of NF was very rare not only the bacteria in this case but also the pathological changes related (involving the mediastinum). The report of his diagnosis and treatment can provide experience for future treatments.
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http://dx.doi.org/10.21037/apm-20-2427DOI Listing
July 2021

The Efficacy and Safety of Programmed Death-1 and Programmed Death Ligand 1 Inhibitors for the Treatment of Hepatocellular Carcinoma: A Systematic Review and Meta-Analysis.

Front Oncol 2021 23;11:626984. Epub 2021 Mar 23.

Department of Clinical Laboratory, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Background: Hepatocellular carcinoma (HCC) is often diagnosed at an advanced stage where only systemic treatment can be offered. The emergence of immune checkpoint inhibitors (ICIs) provides hope for the treatment of HCC. In this study, we performed a meta-analysis to provide evidence for the efficacy and safety of ICIs in the treatment of HCC.

Methods: The following databases and websites were searched: Embase, PubMed, Cochrane Library and ClinicalTrials.gov. The primary endpoints were response rate (RR), disease control rate (DCR), progression-free survival (PFS) and overall survival (OS).

Results: Finally, twelve studies were included in this meta-analysis. When the corresponding outcome indicators and their 95% confidence intervals (CIs) were pooled directly, the overall RR, DCR, PFS and OS were 0.17 (0.15-0.19, I = 56.2%, P=0.009), 0.58 (0.55-0.61, I = 75.9%, P<0.001), 3.27 months (2.99-3.55, I = 73.0%, P=0.001), 11.73 months (10.79-12.67, I = 90.3%, P<0.001). Compared to the control group, treatment with ICIs significantly improved RR, PFS and OS, the OR and HRs were 3.11 (2.17-4.44, P<0.001), 0.852 (0.745-0.974, P=0.019) and 0.790 (0.685-0.911, P=0.001), respectively. However, no significant improvement in DCR was found in ICIs treatment in this meta-analysis.

Conclusion: HCC patients would benefit from ICIs treatment, however, more studies are needed in the future to provide more useful evidence for the treatment of HCC by programmed death-1 (PD-1) or programmed death ligand 1 (PD-L1) inhibitors.
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http://dx.doi.org/10.3389/fonc.2021.626984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021909PMC
March 2021

A supersensitive silicon nanowire array biosensor for quantitating tumor marker ctDNA.

Biosens Bioelectron 2021 Jun 5;181:113147. Epub 2021 Mar 5.

Ministry of Education Engineering Research Center of Smart Microsensors and Microsystems, College of Electronics and Information, Hangzhou Dianzi University, Hangzhou, 310018, China. Electronic address:

Cancer has become one of the major diseases threatening human health and life. Circulating tumor DNA (ctDNA) testing, as a practical liquid biopsy technique, is a promising method for cancer diagnosis, targeted therapy and prognosis. Here, for the first time, a field effect transistor (FET) biosensor based on uniformly sized high-response silicon nanowire (SiNW) array was studied for real-time, label-free, super-sensitive detection of PIK3CA E542K ctDNA. High-response 120-SiNWs array was fabricated on a (111) silicon-on-insulator (SOI) by the complementary metal oxide semiconductor (CMOS)-compatible microfabrication technology. To detecting ctDNA, we modified the DNA probe on the SiNWs array through silanization. The experimental results demonstrated that the as-fabricated biosensor had significant superiority in ctDNA detection, which achieved ultralow detection limit of 10 aM and had a good linearity under the ctDNA concentration range from 0.1 fM to 100 pM. This biosensor can recognize complementary target ctDNA from one/two/full-base mismatched DNA with high selectivity. Furthermore, the fabricated SiNW-array FET biosensor successfully detected target ctDNA in human serum samples, indicating a good potential in clinical applications in the future.
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http://dx.doi.org/10.1016/j.bios.2021.113147DOI Listing
June 2021

BMP5 silencing inhibits chondrocyte senescence and apoptosis as well as osteoarthritis progression in mice.

Aging (Albany NY) 2021 03 19;13(7):9646-9664. Epub 2021 Mar 19.

Department of Orthopedics, Orthopedic Hospital of Guangdong Province, Academy of Orthopedics Guangdong Province, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.

In this study, we using the destabilization of the medial meniscus (DMM) mouse model to investigate the role of bone morphogenetic protein 5 (BMP5) in osteoarthritis (OA) progression mediated via chondrocyte senescence and apoptosis. BMP5 expression was significantly higher in knee articular cartilage tissues of OA patients and DMM model mice than the corresponding controls. The Osteoarthritis Research Society International scores based on histological staining of knee articular cartilage sections were lower in DMM mice where BMP5 was knocked down in chondrocytes than the corresponding controls 4 weeks after DMM surgery. DMM mice with BMP5-deficient chondrocytes showed reduced levels of matrix-degrading enzymes such as MMP13 and ADAMTS5 as well as reduced cartilage destruction. BMP5 knockdown also decreased chondrocyte apoptosis and senescence by suppressing the activation of p38 and ERK MAP kinases. These findings demonstrate that BMP5 silencing inhibits chondrocyte senescence and apoptosis as well as OA progression by downregulating activity in the p38/ERK signaling pathway.
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http://dx.doi.org/10.18632/aging.202708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064147PMC
March 2021

Massage Therapy's Effectiveness on the Decoding EEG Rhythms of Left/Right Motor Imagery and Motion Execution in Patients With Skeletal Muscle Pain.

IEEE J Transl Eng Health Med 2021 3;9:2100320. Epub 2021 Feb 3.

Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen518055China.

Objective: Most of effectiveness assessments of the widely-used Massage therapy were based on subjective routine clinical assessment tools, such as Visual Analogue Scale (VAS) score. However, few studies demonstrated the impact of massage on the Electroencephalograph (EEG) rhythm decoding of Motor imagery (MI) and motion execution (ME) with trunk left/right bending in patients with skeletal muscle pain.

Method: We used the sample entropy (SampEn), permutation entropy (PermuEn), common spatial pattern (CSP) features, support vector machine (SVM) and logic regression (LR) classifiers. We also used the convolutional neural network (CNN) and attention-based bi-directional long short-term memory (BiLSTM) for classification.

Results: The averaged SampEn and PermuEn values of alpha rhythm decreased in almost fourteen channels for five statuses (quiet, MI with left/right bending, ME with left/right bending). It indicated that massage alleviates the pain for the patients of skeletal pain. Furthermore, compared with the SVM and LR classifiers, the BiLSTM method achieved a better area under curve (AUC) of 0.89 for the classification of MI with trunk left/right bending before massage. The AUC became smaller after massage than that before massage for the classification of MI with trunk left/right bending using CNN and BiLSTM methods. The Permutation direct indicator (PDI) score showed the significant difference for patients in different statuses (before vs after massage, and MI vs ME).

Conclusions: Massage not only affects the quiet status, but also affects the MI and ME. Clinical Impact: Massage therapy may affect a bit on the accuracy of MI with trunk left/right bending and it change the topography of MI and ME with trunk left/right bending for the patients with skeletal muscle pain.
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http://dx.doi.org/10.1109/JTEHM.2021.3056911DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7965939PMC
February 2021

Sialic acid-engineered mesoporous polydopamine dual loaded with ferritin gene and SPIO for achieving endogenous and exogenous synergistic T2-weighted magnetic resonance imaging of HCC.

J Nanobiotechnology 2021 Mar 17;19(1):76. Epub 2021 Mar 17.

Department of Radiology, Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, School of Medicine, Lishui Hospital of Zhejiang University, Lishui, 323000, Zhejiang, China.

Background: Hepatocellular carcinoma (HCC) is a common malignant tumor with poor prognosis. Magnetic resonance imaging (MRI) is one of the most effective imaging methods for the early diagnosis of HCC. However, the current MR contrast agents are still facing challenges in the early diagnosis of HCC due to their relatively low sensitivity and biosafety. Thus, the development of effective MR agents is highly needed for the early diagnosis of HCC.

Results: Herein, we fabricated an HCC-targeted nanocomplexes containing SPIO-loaded mesoporous polydopamine ([email protected]), sialic acid (SA)-modified polyethyleneimine (SA-PEI), and alpha-fetoprotein regulated ferritin gene (AFP-Fth) which was developed for the early diagnosis of HCC. It was found that the prepared nanocomplexes ([email protected]/SA-PEI/AFP-Fth) has an excellent biocompatibility towards the liver cells. In vivo and in vivo studies revealed that the transfection of AFP-Fth gene in hepatic cells significantly upregulated the expression level of ferritin, thereby resulting in an enhanced contrast on T2-weighted images via the formed endogenous MR contrast.

Conclusions: The results suggested that [email protected]/SA-PEI/AFP-Fth had a superior ability to enhance the MR contrast of T2-weighted images of tumor region than the other preparations, which was due to its HCC-targeted ability and the combined T2 contrast effect of endogenous ferritin and exogenous SPIO. Our study proved that [email protected]/SA-PEI/AFP-Fth nanocomplexes could be used as an effective MR contrast agent to detect HCC in the early stage.
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http://dx.doi.org/10.1186/s12951-021-00821-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968241PMC
March 2021

Realization of AlSb in the Double-Layer Honeycomb Structure: A Robust Class of Two-Dimensional Material.

ACS Nano 2021 May 16;15(5):8184-8191. Epub 2021 Mar 16.

School of Physics and Wuhan National High Magnetic Field Center, Huazhong University of Science and Technology, Wuhan 430074, China.

Exploring two-dimensional (2D) van der Waals (vdW) systems is at the forefront of materials of physics. Here, through molecular beam epitaxy on graphene-covered SiC(0001), we report successful growth of AlSb in the double-layer honeycomb (DLHC) structure, a 2D vdW material which has no direct analogue to its 3D bulk and is predicted to be kinetically stable when freestanding. The structural morphology and electronic structure of the experimental 2D AlSb are characterized with spectroscopic imaging scanning tunneling microscopy and cross-sectional imaging scanning transmission electron microscopy, which compare well to the proposed DLHC structure. The 2D AlSb exhibits a band gap of 0.93 eV the predicted 1.06 eV, which is substantially smaller than the 1.6 eV of bulk. We also attempt the less-stable InSb DLHC structure; however, it grows into bulk islands instead. The successful growth of a DLHC material here demonstrates the feasibility for the realization of a large family of 2D DLHC traditional semiconductors with characteristic excitonic, topological, and electronic properties.
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http://dx.doi.org/10.1021/acsnano.1c00470DOI Listing
May 2021

Identifying Apoptosis-Related Transcriptomic Aberrations and Revealing Clinical Relevance as Diagnostic and Prognostic Biomarker in Hepatocellular Carcinoma.

Front Oncol 2020 18;10:519180. Epub 2021 Feb 18.

Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research, Lishui Hospital, School of Medicine, Zhejiang University, Lishui, China.

In view of the unsatisfactory treatment outcome of liver cancer under current treatment, where the mortality rate is high and the survival rate is poor, in this study we aimed to use RNA sequencing data to explore potential molecular markers that can be more effective in predicting diagnosis and prognosis of hepatocellular carcinoma. RNA sequencing data and corresponding clinical information were obtained from multiple databases. After matching with the apoptotic genes from the Deathbase database, 14 differentially expressed human apoptosis genes were obtained. Using univariate and multivariate Cox regression analyses, two apoptosis genes (BAK1 and CSE1L) were determined to be closely associated with overall survival (OS) in HCC patients. And subsequently experiments also validated that knockdown of BAK1 and CSE1L significantly inhibited cell proliferation and promoted apoptosis in the HCC. Then the two genes were used to construct a prognostic signature and diagnostic models. The high-risk group showed lower OS time compared to low-risk group in the TCGA cohort (P < 0.001, HR = 2.11), GSE14520 cohort (P = 0.003, HR = 1.85), and ICGC cohort (P < 0.001, HR = 4). And the advanced HCC patients showed higher risk score and worse prognosis compared to early-stage HCC patients. Moreover, the prognostic signature was validated to be an independent prognostic factor. The diagnostic models accurately predicted HCC from normal tissues and dysplastic nodules in the training and validation cohort. These results indicated that the two apoptosis-related signature effectively predicted diagnosis and prognosis of HCC and may serve as a potential biomarker and therapeutic target for HCC.
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http://dx.doi.org/10.3389/fonc.2020.519180DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7931692PMC
February 2021

Identification of QTLs for Salt Tolerance at the Germination and Seedling Stages in Rice.

Plants (Basel) 2021 Feb 24;10(3). Epub 2021 Feb 24.

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.

Rice is highly sensitive to salinity stress during the seedling establishment phase. Salt stress is widely occurring in cultivated areas and severely affects seed germination ability and seedling establishment, which may result in a complete crop failure. The objective of the present study is to identify quantitative trait loci (QTLs) related to salt tolerance of the germination and seedling stages in a rice backcross inbred line (BIL) population that was derived from a backcross of an Africa rice ACC9 as donor and cultivar Zhenshan97 (ZS97) as the recurrent parent. Under salt stress, ACC9 exhibited a higher germination percentage, but more repressed seedling growth than ZS97. Using the BIL population, 23 loci for germination parameters were detected at the germination stage and 46 loci were identified for several morphological and physiological parameters at the seedling stage. Among them, nine and 33 loci with the ACC9 alleles increased salt tolerance at the germination and seedling stages, respectively. Moreover, several major QTLs were found to be co-localized in the same or overlapping regions of previously reported genes for salt stress. These major loci will facilitate improving salt-tolerance rice in genome-breeding programs.
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http://dx.doi.org/10.3390/plants10030428DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7996262PMC
February 2021

Shear stress inhibits cardiac microvascular endothelial cells apoptosis to protect against myocardial ischemia reperfusion injury via YAP/miR-206/PDCD4 signaling pathway.

Biochem Pharmacol 2021 04 18;186:114466. Epub 2021 Feb 18.

Department of Pharmacology, Harbin Medical University-Daqing, Daqing 163319, China. Electronic address:

Cardiac microvascular endothelial cells (CMECs), derived from coronary circulation microvessel, are the main barrier for the exchange of energy and nutrients between myocardium and blood. However, microvascular I/R injury is a severely neglected topic, and few strategies can reverse this pathology. In this study, we investigated the mechanism of shear stress in microvascular I/R injury, and try to elucidate the downstream signaling pathways that inhibit CMECs apoptosis to reduce I/R injury. Our results demonstrated that shear stress inhibited the apoptosis protein, increased PECAM-1 expression and eNOS phosphorylation in hypoxia reoxygenated (H/R) CMECs. The mechanism of shear stress was related to up-regulated expression of YAP, the increased number of YAP entering the nucleus by dephosphorylation, the reduced number of TUNEL positive cells, increased miR-206 and inhibited protein level of PDCD4 in CMECs. However, siRNA-mediated knockdown of YAP abolished the protective effects of shear stress on CMECs apoptosis, similar results obtained from administration with AMO-miR-206, and also prevented PDCD4 (target gene of miR-206) increasing when treatment with both AMO-miR-206 and mimics-miR-206. In vivo, restoring the blood fluid with nitroglycerin (NTG) to mimic in vitro shear stress levels, which subsequently improved cardiac function, reduced infarcted area, lowered microvascular perfusion defects. Functional investigations clearly illustrated that increased the protein expression of PECAM-1 and eNOS phosphorylation, activated YAP, strengthened miR-206 expression, and suppressed PDCD4 expression. In summary, this study confirmed that shear stress reversed CMECs apoptosis, relieved microvascular I/R injury, the mechanism of which involving through YAP/miR-206/PDCD4 signaling pathway to finally suppress myocardial I/R injury.
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http://dx.doi.org/10.1016/j.bcp.2021.114466DOI Listing
April 2021

OsGRETCHENHAGEN3-2 modulates rice seed storability via accumulation of abscisic acid and protective substances.

Plant Physiol 2021 May;186(1):469-482

National Key Laboratory of Crop Genetic Improvement, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.

Seed storability largely determines the vigor of seeds during storage and is significant in agriculture and ecology. However, the underlying genetic basis remains unclear. In the present study, we report the cloning and characterization of the rice (Oryza sativa) indole-3-acetic acid (IAA)-amido synthetase gene GRETCHEN HAGEN3-2 (OsGH3-2) associated with seed storability. OsGH3-2 was identified by performing a genome-wide association study in rice germplasms with linkage mapping in chromosome substitution segment lines, contributing to the wide variation of seed viability in the populations after long periods of storage and artificial ageing. OsGH3-2 was dominantly expressed in the developing seeds and catalyzed IAA conjugation to amino acids, forming inactive auxin. Transgenic overexpression, knockout, and knockdown experiments demonstrated that OsGH3-2 affected seed storability by regulating the accumulation level of abscisic acid (ABA). Overexpression of OsGH3-2 significantly decreased seed storability, while knockout or knockdown of the gene enhanced seed storability compared with the wild-type. OsGH3-2 acted as a negative regulator of seed storability by modulating many genes related to the ABA pathway and probably subsequently late embryogenesis-abundant proteins at the transcription level. These findings shed light on the molecular mechanisms underlying seed storability and will facilitate the improvement of seed vigor by genomic breeding and gene-editing approaches in rice.
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http://dx.doi.org/10.1093/plphys/kiab059DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8154041PMC
May 2021
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