Publications by authors named "K M N"

33 Publications

A novel concept on volumetric assessment and quantification of gastrointestinal bleed on computed tomography angiography: Observations based on a case series.

Ann Hepatobiliary Pancreat Surg 2021 Feb;25(1):160-166

Department of Radiology, Mazumdar Shaw Medical Centre - Narayana Multispecialty Hospital, Narayana Health, Bommasandra, Bengaluru, India.

Department of Radiology, Mazumdar Shaw Medical Centre - Narayana Multispecialty Hospital, Narayana Health, Estimation of quantity and rate of bleeding is of great value in the management of patients with acute GI bleed. Endoscopy and multiphase contrast enhanced computed tomography (MCECT) are the presently employed two important methods for this purpose. Still there is a lacuna in the methods of precise estimation of amount of bleed, which at the moment are somewhat unreliable and subjectively evaluated. We present the value of dynamic contrast-enhanced CT examination in quantitatively estimating the amount of extravasated blood with the help of three illustrative clinical examples. Technique CT examination, the methodology for assessment of quantity of bleed is presented and the discussion of existing literature regarding the estimation methods is presented.Bommasandra, Bengaluru, India.
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http://dx.doi.org/10.14701/ahbps.2021.25.1.160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7952656PMC
February 2021

Physiochemical characterization and toxicity assessment of colloidal mercuric formulation-'Sivanar amirtham'.

Colloids Surf B Biointerfaces 2021 Apr 3;200:111607. Epub 2021 Feb 3.

Department of Chemical Engineering, Anna University, Chennai, 25, India.

The study aims to characterize and understand the toxicological effects of colloidal mercuric formulation. The physiochemical characterization was carried out using Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), Energy dispersive X-ray microanalysis system (EDS), Inductively coupled plasma optical emission spectrometry (ICP-OES), X-ray diffraction (XRD), Zeta potential, Brunauer-Emmett-Teller (BET) and electron microscopy. Based on the physiochemical characterizations, the pairwise relationship between the parameters such as size, surface area, surface charge, reactivity and band gap energy were described. The biological effects of the sample were studied by both in vitro and in vivo assays. The in vitro cytotoxicity assay confirmed that the colloidal mercuric formulation was effective against cancer cells (MCF-7) and less toxic to normal cells (Hek 293). The formulation was effective against MCF-7 with more than 85% of apoptotic and necrotic cells, positive for PI staining when treated with 100 μg/mL. The inflammatory response on the macrophage cell lines was studied. The colloidal mercuric formulation upregulated the expression of TGF-β, IL-6 and TNF-α, due to the presence of arsenic and other organic compounds such as piperine. The in vivo developmental toxicity was observed in Zebrafish hampered growth and survival in a dose and time dependent manner. The formulation was safe at lower concentration and exhibit a dose and time dependent toxicity. Based on the results obtained, it is confirmed that the selective toxicity towards MCF-7 cells is promising to develop an effective formulation for the treatment of cancer, provided more such proofs obtained from in vivo experiments.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111607DOI Listing
April 2021

Effect of Particle Size of Nano-Oxides on Color Stability and Mechanical Properties of Maxillofacial Silicone Elastomers: An In Vitro Study.

Int J Prosthodont 2021 Jan-Feb;34(1):54-60

Purpose: To evaluate the physical and mechanical properties of maxillofacial silicone elastomers following the incorporation of a specific particle size and concentration of nano-titanium dioxide (TiO) and nano-zinc oxide (ZnO).

Materials And Methods: Nano-TiO and nano-ZnO of 20-nm and 40-nm particle sizes and in 1% and 2% concentrations were chosen. Silicone elastomer samples were prepared according to the manufacturer's recommendations. The nano-oxides were incorporated via hand spatulation. The samples were subsequently tested for hardness and color stability and were then subjected to aging in an aging chamber for 1,008 hours, following which they were again evaluated for hardness, color stability, and surface roughness.

Results: A significant change was noted in the physical and mechanical properties post-aging in all the groups. The samples with nano-TiO of 40-nm particle size/2% concentration had the highest hardness, while color stability and surface roughness were higher in samples with nano-TiO of 20-nm particle size/1% and 2% concentrations, respectively.

Conclusion: It can be concluded that 20-nm nano-TiO particles best maintain the ideal properties of maxillofacial silicone elastomers and can potentially be used as alternative opacifiers when mixed with A-2186 maxillofacial silicone elastomers.
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http://dx.doi.org/10.11607/ijp.6593DOI Listing
February 2021

dsDNA-templated fluorescent copper nanoparticles for the detection of lipopolysaccharides.

Anal Methods 2021 01;13(2):186-191

Department of Instrumentation and Applied Physics, Indian Institute of Science Bangalore, India.

The introduction of lipopolysaccharides (LPS) or endotoxins that originate from Gram-negative bacteria into the human blood stream induces a severe immune response that can lead to septic shock, and even death. Hence, the accurate detection of LPS is of great importance in the medical and pharmaceutical sectors. This paper proposes a novel label-free fluorescence assay for the detection of LPS utilizing aptamers and the interference synthesis of dsDNA-templated copper nanoparticles. The assay can be performed at room temperature and does not require expensive reagents. The proposed assay has a limit of detection of 0.95 ng ml-1 of LPS, and the fluorescence emission from the copper nanoparticles was found to vary linearly with the concentration of LPS over a wide range (1 to 105 ng ml-1) with R2 = 0.9877.
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http://dx.doi.org/10.1039/d0ay01906bDOI Listing
January 2021

Heterozygous Frameshift Deletion in a Newborn with a Congenital Heart Anomaly.

Balkan J Med Genet 2020 Jun 26;23(1):83-90. Epub 2020 Aug 26.

Laboratory of Medical Genetics, University Medical Centre Maribor, Maribor, Slovenia.

Kabuki syndrome (KS) is characterized by typical facial features and patients are also affected by multiple congenital anomalies, of which congenital heart anomalies (CHAs) are present in 28.0 to 80.0%. In approximately 75.0% of patients, the genetic causes of KS are caused by mutation in the gene. Although KS is a well-characterized syndrome, reaching the diagnosis in neonates is still challenging. Namely, newborns usually display mild facial features; therefore the diagnosis is mainly based on congenital malformations. In our case, a newborn was referred for next generation sequencing (NGS) testing due to the prenatally observed CHA. After birth, a ventricular septal defect (VSD), vesicoureteral reflux, muscular hypotonia, cleft palate, mild microcephaly, and some dysmorphic features, were noted. The NGS analysis was performed on the proband's genomic DNA using the TruSight One Sequencing Panel, which enriches exons of 4813 genes with clinical relevance to the disease. After variant calling, NGS data analysis was predominantly focused on rare variants in genes involved in VSD, microcephaly, and muscular hypotonia; features observed predominantly in our proband. With the aforementioned protocol, we were able to determine the previously unreported frameshift deletion in the gene resulting in translation termination. Although our proband is a typical representative of KS, his diagnosis was reached only after NGS analysis. Our proband thus represents the importance of genotypephenotype driven NGS analysis in diagnosis of patients with congenital anomalies.
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http://dx.doi.org/10.2478/bjmg-2020-0008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474217PMC
June 2020

Rectal and Bladder Dose Measurements in the Intracavitary Applications of Cervical Cancer Treatment with HDR Afterloading System: Comparison of TPS Data with MOSFET Detector.

J Biomed Phys Eng 2020 Apr 1;10(2):141-146. Epub 2020 Apr 1.

MD, Department of Radiotherapy, J.K.Cancer Institute, Kanpur 208002, Uttar Pradesh, India.

Background: Intracavitary brachytherapy plays a major role in management of cervical carcinoma. Assessment of dose received by OAR's therefore becomes crucial for the estimation of radiation toxicities in HDR brachytherapy.

Objective: Purpose of this study is to evaluate the role of in vivo dosimetry in HDR brachytherapy and to compare actual doses delivered to OAR' s with those calculated during treatment planning.

Material And Methods: In this retrospective study, 50 patients of cervical carcinoma were treated by Microselectron HDR. Out of 50 patients, 26 were treated with a dose of 7 Gy and 24 with a dose of 9 Gy, prescribed to point A. Brachytherapy planning and evaluation of dose to bladder and rectum was done on TPS & in vivo dosimetry was performed using portable MOSFET.

Results: Calibration factors calculated for both dosimeters are almost equal and are 0.984 cGy/mV and 1.0895 cGy/mV. For bladder, dose deviation was found to be within ± 5% in 28 patients, ± 5-10% in 14 patients, ± 10-15% in 4 patients. Deviation between TPS-calculated dose and dose measured by MOSFET for rectum was within ± 5% in 31 patients, ± 5-10% in 8 patients, and ± 10-15% in 7 patients.

Conclusion: TPS calculated doses were slightly higher than that measured by MOSFET. The use of small size MOSFET dosimeter is an efficient method for accurately measuring doses in high-dose gradient fields typically seen in brachytherapy. Therefore, to reduce the risk of large errors in dose delivery, in vivo dosimetry can be done in addition to TPS computations.
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http://dx.doi.org/10.31661/jbpe.v0i0.1065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7166216PMC
April 2020

Programmed Death Ligand 1; An Immunotarget for Renal Cell Carcinoma.

Asian Pac J Cancer Prev 2019 Oct 1;20(10):2951-2957. Epub 2019 Oct 1.

Department of Physiology, SRMC and RI, Porur, Chennai, India.

Background: In this era of developing targeted therapies and immunotherapies as a treatment for renal cell carcinoma (RCC), Programmed death ligand 1 (PDL1) as a novel biomarker for RCC is analysed in our study. About 90% of all renal cancers are Renal Cell Carcinoma. Most cases are diagnosed incidentally. 17% of cases are advanced at the time of diagnosis. PDL1 being a trans-membrane cell surface protein is expressed on the tumor cells and is found to have a chief role to inhibit the T cell immune response. It is essential to improve the host immunity by targeting the PD1/PDL1 pathway, thereby destroying the tumor progression.

Aim: The aim of this study was to evaluate the expression of PDL1 in tumor cells and adjacent normal tissue among the renal cell carcinoma patients and assess the relation between the PDL1 expression and the tumor characters.

Methods: This is a retrospective study. Ethical clearance was obtained from the institution. 150 histopathologically proven RCC cases were chosen. Immunohistochemistry using a PD-L1 rabbit monoclonal antibody was performed on paraffin embedded formalin fixed tissue blocks. Q scoring was done to calculate the expression of PDL1.

Statistical Analysis: Chi square test was done to assess the comparison between the PDL1 expression in tumor cells and their characteristic features like histology, grade and stage. SPSS (version 20.0) was used for analysis. P value <0.05 was considered significant. It also explains the heterogenous nature of PDL1 as it expressed more in the aggressive pathologic characters like high grade.

Results: Positive PD-L1 expression was seen in 44% of tumors. Significant association was observed between high WWHO ISUP grading and positive PDL1 expression (p=0.028). It was expressed in 75% of the sarcomatous type of RCC and 46.8% of clear cell RCCs.

Conclusion: Our study suggests that blocking PD1/PDL1 pathway may become an effective mode of treatment in cancer immunotherapy especially for Renal Cell Carcinomas. Our findings confirmed the significant association between expression of PDL1 and the high graded tumors which proves it to be an important prognostic factor.
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http://dx.doi.org/10.31557/APJCP.2019.20.10.2951DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982669PMC
October 2019

Innocuous clinical presentation of a SMARCA4-deficient thoracic sarcoma arising in a patient with chronic empyema thoracis.

Pathology 2019 Oct 27;51(6):657-659. Epub 2019 Aug 27.

Department of Pathology, All India Institute of Medical Sciences, New Delhi, India. Electronic address:

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http://dx.doi.org/10.1016/j.pathol.2019.05.011DOI Listing
October 2019

Pooled nucleic acid testing strategy for monitoring HIV-1 treatment in resource limited settings.

J Clin Virol 2019 08 30;117:56-60. Epub 2019 May 30.

YR Gaitonde Centre for AIDS Research and Education, Chennai, India. Electronic address:

Background: Virological monitoring (VM) and drug resistance (DR) analysis are crucial for effective HIV management. Due to the high cost of commercial assays, VM and DR analysis is not performed in resource-limited-settings.

Objective: The objective of this study is to develop a pooling based algorithm for the combined identification of virologic treatment failure (VTF) by nucleic acid testing (NAT) and DR by sequencing - NAT+DR assay.

Study Design: We enrolled 559 participants on first-line therapy and analyzed for VTF. The virologically suppressed participants were followed-up to see the VTF prevalence (>1000 copies/mL) and DR by the NAT+DR pooling. Each pool comprising 5 plasma samples were amplified by targeting reverse transcriptase gene, if found positive, the pool was deconvoluted and samples were individually tested for HIV RNA and DR. Assay characteristics of NAT+DR assay were calculated in comparison with commercial assay.

Results: Of 559 participants, 67 had VTF at baseline and were excluded. Of the remaining 478 participants, 325 returned for follow-up and NAT+DR assay was performed for them. Of 65 pools tested, 13 pools were positive. On deconvolution 14 individuals were found to have VTF. Sensitivity, specificity, positive predictive value and negative predictive value was 100%, relative efficiency was 59% and 87% & 85% cost was saved for identifying VTF and combined identification of VTF and DR, respectively.

Conclusions: Pooled NAT+DR assay is likely a good strategy to drastically reduce the cost and sustainability of the VM and can thereby facilitate the scale-up of successful HIV treatment programs, and reduce unnecessary switching to second-line drugs in resource-limited-settings.
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http://dx.doi.org/10.1016/j.jcv.2019.05.012DOI Listing
August 2019

In silico evaluation of multispecies toxicity of natural compounds.

Drug Chem Toxicol 2019 May 21:1-7. Epub 2019 May 21.

c Department of Biotechnology , Vels Institute of Science, Technology and Advanced Studies , Chennai , India.

Natural compounds are widely explored in industries, as a lead compound. Evaluating their toxicity is of utmost importance, as they may cause other side effects. The major hassles in evaluating the toxicity of compounds through in vivo and in vitro methods such as time, money, workforce, and use of animal models can be overcome by computational methods. Quantitative structure-activity relationship (QSAR) models predict the toxicity from the structure of a compound. In the present study, the methanolic extracts of three plants, namely, Carissa carandas, Canthium angustifolium, and Epiphyllum oxypetalum, were subjected to Gas Chromatography-Mass Spectrometry (GC-MS), in which 27 different compounds were identified. The compounds were evaluated for their toxicity through QSAR-Toxicity Estimation Software Tool (TEST) against multispecies - Daphnia magna, Pimephales promelas, Tetrahymena pyriformis, and rat (Oral). The study revealed that the order of toxicity of the natural compounds was D. magna > T. pyriformis > P. promelas > Rat (Oral). All the compounds were non-bioaccummulative, while most of them were developmental toxicants. Only one compound (Dasycarpidan-1-methanol, acetate (ester)) was a mutagen. Further studies of the compounds on in vivo models are recommended after in silico analysis, for exploration in different industries.
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http://dx.doi.org/10.1080/01480545.2019.1614023DOI Listing
May 2019

The Frequency of EGFR and KRAS Mutations in the Turkish Population with Non-small Cell Lung Cancer and their Response to Erlotinib Therapy.

Balkan J Med Genet 2018 Dec 31;21(2):21-26. Epub 2018 Dec 31.

Medical Biology Department, Pamukkale University, Denizli Turkey.

In this study, profiles of epidermal growth factor receptor (EGFR) and Kirsten ras sarcoma (KRAS) mutations and response to erlotinib therapy have been investigated in patients with non-small cell lung cancer (NSCLC). DNA from 300 patients with NSCLC was extracted from paraf-fin-embedded tissues. After the extracted DNA was sequenced by pyrosequencing method, a total of 97 (32.0%) patients out of 300 were detected to carry an EGFR mutation and 75 (25.0%) patients out of 300 carried a KRAS mutation; 20 (6.6%) patients were detected to carry both of EGFR and KRAS mutations. The EGFR mutations were found to be statistically significant in female patients (48.0 women . 28.0% men, non smokers (49.0 . 26.0%) and adenocarcinoma (37.8 . squamous 26.8%). The overall rate of survival in patients receiving erlotinib therapy than in patients who did not. In patients without the KRAS mutation, the median overall survival rate was 161 ± 30 weeks with erlotinib therapy and 90 ± 13 weeks in patients without erlotinib therapy. In patients having KRAS mutation, the median overall survival was 98 ± 16 weeks with erlotinib therapy and 34 ± 16 weeks with no erlotinib therapy. In our study, we once again demonstrated that the presence of these mutations affected response to erlotinib therapy. The KRAS mutations negatively affected survival rate with and without erlotinib therapy.
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http://dx.doi.org/10.2478/bjmg-2018-0022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454234PMC
December 2018

Enhancement of the fluorescence properties of double stranded DNA templated copper nanoparticles.

Mater Sci Eng C Mater Biol Appl 2019 May 14;98:1034-1042. Epub 2019 Jan 14.

Department of Instrumentation and Applied Physics, Indian Institute of Science Bangalore. Electronic address:

The weak fluorescence emission from dsDNA templated copper nanoparticles necessitates the use of high-end detectors like photomultiplier tubes for their detection. This sets limitations on their applicability to in-situ analyte detection and point-of-care applications which utilize comparatively low cost and less sensitive detectors. In this article, a technique to improve the fluorescence properties of copper nanoparticles templated on dsDNA is reported. The fluorescence enhancement is achieved by introducing a modification in the conventional synthesis technique by using a combination of sodium ascorbate and Taq buffer. When compared to the existing methods, the proposed method achieves 11 times higher fluorescence signal intensity from the dsDNA templated copper nanoparticles and 4 times faster attainment of maximum fluorescence signal. The effect of the ionic strength of the individual constituent components of Taq buffer on the fluorescence emission from the copper nanoparticles is also studied here. The utility of this enhancement strategy for analyte measurement is demonstrated with the example of melamine detection from milk samples. A linear relationship was observed between the fluorescence intensity from the copper nanoparticles and the concentration of melamine in the range from 0.5 ppm to 100 ppm (R = 0.9919), with a limit of detection of 0.1 ppm. The reported fluorescence enhancement technique also results in 2.95 times improved sensitivity of detection when compared to the conventional technique.
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http://dx.doi.org/10.1016/j.msec.2019.01.042DOI Listing
May 2019

The development and application of a method for assessing radionuclide surface contamination density based on measurements of ambient dose equivalent rate.

J Radiol Prot 2019 Jun 29;39(2):354-372. Epub 2019 Jan 29.

State Research Center-Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency (SRC-FMBC), 123182, Zhivopisnaya st. 46, Moscow, Russia.

This article presents a method for assessing the radionuclide surface contamination density (SCD) on open sites and in premises of a radiation hazardous facility based on measurements of the ambient dose equivalent rate (ADER). The method is intended for use at the initial stage of the assessment of the radiation environment at facilities. The assessed SCD at a given location on the surface can differ from the directly measured SCD at that location, since sources located on the surface and distributed by the depth contribute to the ADER value. The method makes it possible to estimate SCD with reasonable accuracy without increasing the number of measurements, and thus avoid additional occupational exposure and the use of additional resources. SCD and ADER as spatial variables have different support of measurement data. For ADER, measured at a height of 1 m, the support of measurement data can be taken to be a circle in the centre of which a gamma-ray detector is located, with a radius of several tens of meters. In contrast, SCD has the support of measurement data, close to the overall dimensions of the beta detector (100 cm). To solve the problem of SCD calculation on the basis of ADER measurements, the method of conversion coefficients (MCC) is usually applied, based on the use of conversion factors; however, this method provides an adequate estimate only under conditions of an SCD with low gradient over the surface. The method proposed in this article is applicable for an arbitrary distribution of SCD, and designed to deal with heterogeneous contamination patterns. The developed method is based on the numerical solution of the Fredholm equation of the first kind. The measurement data always contain an error, therefore, the task of the SCD calculation is an ill-posed problem, and the Tikhonov regularisation method (ridge regression) was used to solve it. The article presents the method developed and examples of use. Validation of the method was performed using 38 measurements of the radioactive contamination from Cs in soil. It is shown that the method proposed in the article demonstrates a significant superiority in comparison with the MCC method, because it allows more accurate localisation of areas contaminated with radionuclides and is applicable for an arbitrary distribution of SCD.
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http://dx.doi.org/10.1088/1361-6498/ab02aaDOI Listing
June 2019

Breast Cancer Detection using Crow Search Optimization based Intuitionistic Fuzzy Clustering with Neighborhood Attraction

Asian Pac J Cancer Prev 2019 Jan 25;20(1):157-165. Epub 2019 Jan 25.

Department of Computer Technology, Kongu Engineering College, Perundurai, Tamilnadu, India. Email:

Objective: Generally, medical images contain lots of noise that may lead to uncertainty in diagnosing the abnormalities. Computer aided diagnosis systems offer a support to the radiologists in identifying the disease affected area. In mammographic images, some normal tissues may appear to be similar to masses and it is tedious to differentiate them. Therefore, this paper presents a novel framework for the detection of mammographic masses that leads to early diagnosis of breast cancer. Methods: This work proposes a Crow search optimization based Intuitionistic fuzzy clustering approach with neighborhood attraction (CrSA-IFCM-NA) for identifying the region of interest. First order moments were extracted from preprocessed images. These features were given as input to the Intuitionistic fuzzy clustering algorithm. Instead of randomly selecting the initial centroids, crow search optimization technique is applied to choose the best initial centroid and the masses are separated. Experiments are conducted over the images taken from the Mammographic Image Analysis Society (mini-MIAS) database. Results: CrSA-IFCM-NA effectively separated the masses from mammogram images and proved to have good results in terms of cluster validity indices indicating the clear segmentation of the regions. Conclusion: The experimental results show that the accuracy of the proposed method proves to be encouraging for detection of masses. Thus, it provides a better assistance to the radiologists in diagnosing breast cancer at an early stage.
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http://dx.doi.org/10.31557/APJCP.2019.20.1.157DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6485576PMC
January 2019

Distributed ICSA Clustering Approach for Large Scale Protein Sequences and Cancer Diagnosis

Asian Pac J Cancer Prev 2018 Nov 29;19(11):3105-3109. Epub 2018 Nov 29.

Department of Computer Applications, Selvam College of Technology, Namakkal, India. Email:

Objective: With the over saturating growth of biological sequence databases, handling of these amounts of data has increasingly become a problem. Clustering has become one of the principal research objectives in structural and functional genomics. However, exact clustering algorithms, such as partitioned and hierarchical clustering, scale relatively poorly in terms of run time and memory usage with large sets of sequences. Methods: From these performance limits, heuristic optimizations such as Cuckoo Search Algorithm with genetic operators (ICSA) algorithm have been implemented in distributed computing environment. The proposed ICSA, a global optimized algorithm that can cluster large numbers of protein sequences by running on distributed computing hardware. Results: It allocates both memory and computing resources efficiently. Compare with the latest research results, our method requires only 15% of the execution time and obtains even higher quality information of protein sequence. Conclusion: From the experimental analysis, We noticed that the cluster of large protein sequence data sets using ICSA technique instead of only alignment methods reduce extremely the execution time and improve the efficiency of this important task in molecular biology. Moreover, the new era of proteomics is providing us with extensive knowledge of mutations and other alterations in cancer study.
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http://dx.doi.org/10.31557/APJCP.2018.19.11.3105DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318385PMC
November 2018

Distribution based Fuzzy Estimate Spectral Clustering for Cancer Detection with Protein Sequence and Structural Motifs

Asian Pac J Cancer Prev 2018 Jul 27;19(7):1935-1940. Epub 2018 Jul 27.

Department of Computer Applications, Selvam College of Technology, Namakkal, TamilNadu, India. Email:

Objective: In biological data analysis, protein sequence and structural motifs are an amino-acid sequence patterns that are widespread and used as tools for detecting the cancer at an earlier stage. To improve the cancer detection with minimum space and time complexity, Distribution based Fuzzy Estimate Spectral Clustering (DFESC) technique is developed. Methods: Initially, the protein sequence motifs are taken from dataset to form the cluster. The Distribution based spectral clustering is applied to group the protein sequence by measuring the generalized jaccard similarity between each protein sequences. To develop the clustering accuracy, soft computing technique namely fuzzy logic is applied to calculate membership value of each sequence motifs. Results: The outcome showed that the presented DFESC technique effectively identifies the cancer in terms of clustering accuracy, false positive rate, and cancer detection time and space complexity. Conclusion: Based on the observations, evaluation of DFESC technique provides improved result for premature detection of cancer using protein sequence and structural motifs.
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http://dx.doi.org/10.22034/APJCP.2018.19.7.1935DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165630PMC
July 2018

Thermoelectric properties of Se and Zn/Cd/Sn double substituted CoSb skutterudite compounds.

Phys Chem Chem Phys 2017 Oct;19(41):28116-28126

Department of Physics, Anna University, Chennai - 600025, India.

Double-doped skutterudite CoSb compounds are reported as a good n-type thermoelectric system which operate in the mid-temperature range. Instead of filling the skutterudite phase to minimize the thermal conductivity, it is proposed to induce disorder in the pnicogen rings by the substitution of Zn/Cd/Sn and Se. Structural analyses of the prepared compounds were carried out by Rietveld refinement process. The compounds show overall reduction in thermal conductivity, particularly the lattice part. Since vibration modes of heat-carrying phonons predominantly fall within the frequency range of the pnicogen rings, double substitutions on those pnicogen rings are particularly helpful in distracting the thermal transport. As larger mass difference and strain fluctuations can more efficiently scatter the heat-carrying phonons through short mean free path, it drastically restrains the thermal transport of the compounds, and this effect has been successfully demonstrated using the Debye-Callaway-Klemens model. Smooth electrical transport behavior is observed in all the samples and the power factor values are quite comparable to reported values. Phonon scattering mechanism and local distortion in the structure of the compounds is also evaluated by Raman analysis. Collectively, a high peak ZT of ∼0.7 and ∼0.65 at 673 K is obtained for CoSbSeZn and CoSbSeSn compounds which shows more than 50% enhancement relative to the pristine CoSb system.
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http://dx.doi.org/10.1039/c7cp04634kDOI Listing
October 2017

Disseminated BCG Infectious Disease and Hyperferritinemia in a Patient With a Novel NEMO Mutation.

J Investig Allergol Clin Immunol 2016 ;26(4):268-271

Ege University School of Medicine, Department of Pediatrics, Division of Pediatric Immunology, Izmir, Turkey.

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http://dx.doi.org/10.18176/jiaci.0068DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5038349PMC
September 2016

Acute effects of 3G mobile phone radiations on frontal haemodynamics during a cognitive task in teenagers and possible protective value of Om chanting.

Int Rev Psychiatry 2016 06 7;28(3):288-98. Epub 2016 Jun 7.

c S-VYASA Yoga University , Bangalore , India.

Mobile phone induced electromagnetic field (MPEMF) as well as chanting of Vedic mantra 'OM' has been shown to affect cognition and brain haemodynamics, but findings are still inconclusive. Twenty right-handed healthy teenagers (eight males and 12 females) in the age range of 18.25 ± 0.44 years were randomly divided into four groups: (1) MPONOM (mobile phone 'ON' followed by 'OM' chanting); (2) MPOFOM (mobile phone 'OFF' followed by 'OM' chanting); (3) MPONSS (mobile phone 'ON' followed by 'SS' chanting); and (4) MPOFSS (mobile phone 'OFF' followed by 'SS' chanting). Brain haemodynamics during Stroop task were recorded using a 64-channel fNIRS device at three points of time: (1) baseline, (2) after 30 min of MPON/OF exposure, and (3) after 5 min of OM/SS chanting. RM-ANOVA was applied to perform within- and between-group comparisons, respectively. Between-group analysis revealed that total scores on incongruent Stroop task were significantly better after OM as compared to SS chanting (MPOFOM vs MPOFSS), pre-frontal activation was significantly lesser after OM as compared to SS chanting in channel 13. There was no significant difference between MPON and MPOF conditions for Stroop performance, as well as brain haemodynamics. These findings need confirmation through a larger trial in future.
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http://dx.doi.org/10.1080/09540261.2016.1188784DOI Listing
June 2016

Primary Transanal Management of Rectal Atresia in a Neonate.

J Neonatal Surg 2016 Apr-Jun;5(2):20. Epub 2016 Apr 10.

Pediatric Surgery Department, Fattouma Bourguiba Hospital, Monastir University, Tunisia.

Rectal atresia (RA) with a normal anus is a rare anomaly. We describe a case of rectal atresia in a newborn male presenting with an abdominal distension and failure of passing meconium. The rectal atresia was primarily operated by transanal route.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4841376PMC
April 2016

Analysis of Food Pairing in Regional Cuisines of India.

PLoS One 2015 2;10(10):e0139539. Epub 2015 Oct 2.

Center for Biologically Inspired System Science, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan, India.

Any national cuisine is a sum total of its variety of regional cuisines, which are the cultural and historical identifiers of their respective regions. India is home to a number of regional cuisines that showcase its culinary diversity. Here, we study recipes from eight different regional cuisines of India spanning various geographies and climates. We investigate the phenomenon of food pairing which examines compatibility of two ingredients in a recipe in terms of their shared flavor compounds. Food pairing was enumerated at the level of cuisine, recipes as well as ingredient pairs by quantifying flavor sharing between pairs of ingredients. Our results indicate that each regional cuisine follows negative food pairing pattern; more the extent of flavor sharing between two ingredients, lesser their co-occurrence in that cuisine. We find that frequency of ingredient usage is central in rendering the characteristic food pairing in each of these cuisines. Spice and dairy emerged as the most significant ingredient classes responsible for the biased pattern of food pairing. Interestingly while individual spices contribute to negative food pairing, dairy products on the other hand tend to deviate food pairing towards positive side. Our data analytical study highlighting statistical properties of the regional cuisines, brings out their culinary fingerprints that could be used to design algorithms for generating novel recipes and recipe recommender systems. It forms a basis for exploring possible causal connection between diet and health as well as prospection of therapeutic molecules from food ingredients. Our study also provides insights as to how big data can change the way we look at food.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0139539PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4592201PMC
June 2016

Isolation and identification of culturable bacteria from honeydew of whitefly, Bemisia tabaci (G.) (Hemiptera: Aleyrodidae).

Meta Gene 2014 Dec 14;2:114-22. Epub 2014 Jan 14.

Division of Horticulture, ICAR, Krishi Anusandhan Bhawan-II, New Delhi 110 012, India.

Bemisia tabaci (G.) is an important pest and a vector of Gemini viruses infecting plants. During the process of feeding B. tabaci excretes honeydew which is rich in nutrients, and an excellent medium for microbial growth. Recent report proved that volatile emitted by the honeydew associated bacteria of aphid, Acyrthosiphon pisum Harris was involved in natural enemy calling. Thus understanding the honeydew associated bacteria is of paramount importance from the non-chemical method of insect pest management. In this perspective, very less information is available on bacteria associated with the honeydew excreted by B. tabaci. Therefore, in the present study we have isolated and characterized three culturable bacteria from the honeydew of B. tabaci viz. Bacillus endophyticus, Bacillus niacini and Roseomonas species by employing 16Sr DNA BLASTx analyses which revealed that both B. endophyticus and B. niacini had high similarity (> 99%) to the respective species, while Roseomonas sp. showed only 95% similarity to the existing Roseomonas sp. specificity of honeydew association of Roseomonas sp. was confirmed by developing specific primers as this genus is reported from immunocompromised persons and recently from ticks and mites. The present study also indicated the possible host-plant origin of these honeydew associated bacteria.
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http://dx.doi.org/10.1016/j.mgene.2013.11.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4287792PMC
December 2014

A positive association between foot posture index and medial compartment knee osteoarthritis in moroccan people.

Open Rheumatol J 2014 11;8:96-9. Epub 2014 Dec 11.

Rheumatology Department, Hassan II University Hospital, Fez, Morocco.

Objectives: To compare foot posture in people with and without medial compartment knee osteoarthritis (OA), and to assess association between its abnormalities and medial compartment knee OA.

Methods: We compared the foot posture of patients with clinically and radiographically-confirmed medial compartment knee OA and asymptomatic healthy controls using the foot posture index (FPI), navicular height, and the medial arch.

Results: We included 100 patients and 80 asymptomatic controls. The mean age of patients was 59 ± 7 (44-76) years and 48 ± 9 (28-60) years in the control (p=0.06). Patients group have more pronated foot for FPI (1.50 ± 2.68 vs 0.72 ± 2.63; p=0.05), more flat foot (42% vs 22%; p=0.03), and less pes cavus than the control group (58% vs 77%; p=0.004). However, there was no significant difference between the groups in the navicular height (3.90 ± 0.85 cm vs 4.00 ± 0.76 cm; p=0.41). In multivariate statistical analysis, after adjusting for age and body mass index, pronated foot in FPI (OR=1.22, 95%IC= [1.06-1.40], p=0.005), and pes cavus (OR=0.32, 95%IC= [0.11-0.93], p=0.03) had a significant correlation with the knee osteoarthritis.

Conclusion: Pronated foot posture and flat foot are significantly associated with medial compartment knee osteoarthritis.
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http://dx.doi.org/10.2174/1874312901408010096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4279032PMC
January 2015

Influence of rotating shift work on visual reaction time and visual evoked potential.

J Clin Diagn Res 2014 Oct 20;8(10):BC04-7. Epub 2014 Oct 20.

Professor and HOD, Department of Physiology, SRM MCH & RC , Chennai, India .

Background: The present day life style is changing the circadian rhythm of the body especially in rotating night shift workers. The impact of this prolongs their reaction time. Night shift also interferes with the circadian variation of pupil size which may affect the visual evoked potential.

Aim: To compare the visual reaction time, visual evoked potential (VEP) in rotating night shift workers & day workers and also to correlate the changes in visual reaction time with visual evoked potential.

Materials And Methods: Forty healthy male security guards & staff (25 - 35 y) who did rotating night shifts at least for six months & 40 d workers (25 - 35 y) who did not do night shift in last two years were involved in the study. Visual reaction time and the latency & amplitude of VEP were recorded.

Result: Kolmogorov- Smirnov test for normalcy showed the latencies & amplitude of VEP to be normally distributed. Student's unpaired t test showed significant difference (p<0.05) in the visual time and in the latencies of VEP between night shift & day workers. There was no significant difference in the amplitude of VEP.

Conclusion: Night shift workers who are prone to circadian rhythm alteration will have prolonged visual reaction time & visual evoked potential abnormalities. Implementation of Bright Light Therapy would be beneficial to the night shift worker.
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http://dx.doi.org/10.7860/JCDR/2014/9399.4992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4253150PMC
October 2014

Dexamethasone as an additive to bupivacaine in fascia lliaca compartment block: a prospective, randomized and double blind study.

J Clin Diagn Res 2014 Aug 20;8(8):GC05-8. Epub 2014 Aug 20.

Tutor, Department of Anaesthesiology, Sri Devaraj Urs Medical College , SDUAHER, Tamaka, Kolar, Karnataka, India .

Background: Patients with fracture femur experience severe pain on movement during positioning for spinal anaesthesia. Fascia Iliaca Compartment Block (FICB) has been used effectively for providing analgesia during positioning of the patient for spinal anaesthesia.

Aim: To test the hypothesis that, adding dexamethasone would significantly prolong the duration of Bupivacaine in FICB.

Materials And Methods: Sixty patients aged 18 to 80 years posted for ORIF (Open Reduction and Internal Fixation) of fracture femur were included to receive FICB. This was a prospective, randomized, double blind study done at tertiary medical college hospital. Thirty patients received 38ml of 0.25 % bupivacaine with 2ml saline and another 30 patients received 38ml of 0.25 % bupivacaine with 2ml dexamethasone (8mg). Thirty minutes after FICB, patient satisfaction during positioning for spinal anesthesia was recorded. In the post-operative period, duration of analgesia and the total doses of rescue analgesics were recorded in both the groups.

Results: Patients who received Bupivacaine with dexamethasone had significant prolongation of analgesia and required fewer doses of rescue analgesics as compared to patients who received Bupivacaine alone for FICB. However, the onset of analgesia, VAS scores and patient satisfaction during positioning for spinal anaesthesia were similar in both groups.

Conclusion: Our study shows that adding Dexamethasone (8mg) to Bupivacaine for FICB significantly prolonged the duration of block and decreased the requirement of rescue analgesics as compared to patients who received Bupivacaine alone. FICB is relatively easy and safe to perform. In our study we did not encounter any complication while doing the procedures and also by adding dexamethasone.
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http://dx.doi.org/10.7860/JCDR/2014/8754.4687DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4190730PMC
August 2014

The association of hypomagnesaemia, high normal uricaemia and dyslipidaemia in the patients with diabetic retinopathy.

J Clin Diagn Res 2013 Sep 10;7(9):1852-4. Epub 2013 Sep 10.

Assistant Professor, Department of Biochemistry, Chettinad Hospital & Research Institute , Chennai-603103, India .

Context: Diabetic retinopathy is fast becoming an important cause of a visual disability. The visual disability which results from diabetes is a significant public health problem; however, this morbidity is largely preventable and treatable. If it is managed with a timely intervention, the quality of life can be preserved.

Aims: The objective of this study was to investigate the association of serum uric acid, magnesium and the lipid profile in diabetic retinopathy with Normal subjects and Diabetes mellitus without retinopathy, among the south Indian population.

Settings And Design: The diabetic retinopathy patients were identified from the diabetic health camps which were held in rural areas, and they were compared with those with diabetes without complications and the normal subjects.

Material And Methods: The diabetic retinopathy patients were compared with the healthy subjects and with diabetes without retinopathy. Furthermore, the Diabetic retinopathy patients were grouped as proliferative and non-proliferative, based on the fundoscopic findings. Magnesium, uric acid, FPG, fructosamine and the lipid profile were measured in the above groups and they were analyzed.

Statistical Analysis: The statistical analysis was done by using the SPSS software, by applying the Student 't' test.

Results: The mean serum magnesium concentration was observed to be low in the diabetic retinopathy group (1.43mg/dl) as compared to those in the controls and the diabetic subjects. The serum Uric acid concentration was high normal (4.84mg/dl), which was associated with the dyslipidaemia in diabetic retinopathy.

Conclusion: The poor glycaemic control in diabetes is associated with hypomagnesaemia, and increased uric acid concentration with dyslipidaemia, which can be an initial picture of the ongoing biochemical changes in the complication of diabetes, which can help in predicting the onset of diabetic retinopathy in diabetes.
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http://dx.doi.org/10.7860/JCDR/2013/6106.3332DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3809619PMC
September 2013

Anti cariogenic efficacy of herbal and conventional tooth pastes - a comparative in-vitro study.

J Int Oral Health 2013 Apr;5(2):8-13

Oral Pathology and Microbiology, Department of Oral Pathology and Microbiology, College of Dental Sciences, Davangere, Karnataka, India.

Background: An upsurge of herbal products in various catalogues of fast moving consumer goods is evident. Dental creams or pastes which have numerous brands since years, have addition of many more herbal tooth pastes. Main claim of these herbal tooth pastes being effective reduction in cavities and plaque control. Proven fact is that proper brushing with a tooth brush and tooth paste brings down the caries incidence, and there is a substantial amount of contribution made by indispensable ingredient i.e, tooth pastes and their antibacterial component.

Aim: To evaluate the antibacterial efficacy of various herbal tooth pastes available in the market and compare it with a conventional tooth paste with known antibacterial effect.

Materials & Methods: The antibacterial efficacy of five herbal tooth pastes and two conventional tooth pastes with different ingredients was evaluated by the zone of inhibition created around the disc on the culture plates against streptococcus mutans and lactobacillus acidophilus.

Results: The herbal tooth pastes showed similar efficacy as that of the conventional tooth pastes. One herbal tooth paste with multiple herbal ingredients had greater zone of inhibition compared to the conventional tooth pastes and other herbal tooth pastes.

Conclusion: Herbal tooth pastes have similar antibacterial effect as conventional tooth pastes. Tooth paste with multiple herbal ingredients is more efficient than the tooth pastes with fewer herbal ingredients in an anticariogenic property. Key words: Anti-carcinogenicity, Herbal tooth pastes, Plaque Control. How to cite this article: Mohan Kumar K P, Priya N K, Madhushankari G S. Anti Cariogenic Efficacy of Herbal and Conventional Tooth Pastes - A Comparative In-Vitro Study. J Int Oral Health 2013; 5(2):8-13.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768065PMC
April 2013

Pomegranate Extracts in the Management of Men's Urologic Health: Scientific Rationale and Preclinical and Clinical Data.

Evid Based Complement Alternat Med 2013 26;2013:701434. Epub 2013 Mar 26.

Department of Urology, Institute of Urologic Oncology, David Geffen School of Medicine at the University of California, 924 Westwood Boulevard, Suite 1050, Los Angeles, CA 90095-7384, USA ; Department of Urology, University Medicine Greifswald, F.-Sauerbruch-Str., 17 489 Greifswald, Germany.

Multiple strands of research provide growing evidence that diet, nutrition, and life style play a role in the development and the course of urological diseases. Numerous micronutrients and polyphenols found in soy, green tea, and many fruits and vegetables have been described to impact diseases including erectile dysfunction, benign prostatic hyperplasia, and prostate cancer. However, oftentimes these reports lack both a scientific rationale and supportive evidence base. The efficacy of pomegranate, on the other hand, in the modulation of central biological processes like inflammation, hypoxia, and oxidative stress that are important in the pathogenesis of urological maladies has been robustly demonstrated in preclinical in vitro and in vivo studies. Moreover, clinical trials have further supported its use in the treatment of several diseases, in particular in the management of prostate cancer. Herein, we critically review the scientific knowledge about the current role and future prospects for the use of pomegranate extracts in the therapy of erectile dysfunction, benign prostatic hyperplasia, and prostate cancer.
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http://dx.doi.org/10.1155/2013/701434DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3622365PMC
April 2013

The Peak Expiratory Flow Rate (PEFR): the Effect of Stress in a Geriatric Population of Chennai- A Pilot Study.

J Clin Diagn Res 2013 Feb 14;7(2):409-10. Epub 2013 Jan 14.

Assistant Professor, Department of Physiology, Sree Balaji Medical College and Hospital , Chromepet, Chennai, India .

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http://dx.doi.org/10.7860/JCDR/2013/5356.2728DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592329PMC
February 2013