Publications by authors named "K Lakhchev"

2 Publications

  • Page 1 of 1

[Genetic control of yeast cell morphology].

Genetika 1994 Aug;30(8):1036-42

Yeast cell phenotype and morphology result from the interaction of two processes, polarized growth, on the one hand, and cytokinesis with subsequent division of the cell wall, on the other. Imbalance of the equilibrium of these two principal processes may lead to the occurrence of yeast morphological mutants that generate rough colonies. Two types of such mutants exist, Rgh and Rpm. The Rgh mutants have ellipsoid cells and either form chains or generate clumps at high frequency. These changes in heterothallic strains of saccharomyces occur due to recessive mutations in any of 11 non-linked genes. In homothallic strains of saccharomyces, the trait Rgh depends on the complementary interaction of one dominant and two recessive genes. Rpm mutants generate rough colonies, which contain elongated cells that give rise to pseudomycelium. The mutants obtained are recessive and occur in six non-linked genes. The Rpm mutants represent an example of the manifestation of mycelium-yeast dimorphism in fungi, and this case may be regarded as a simple morphogenetic model.
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August 1994

[Cloning and inactivation of a chromosomal copy of the imidazole glycerophosphate dehydratase (HIS) gene from Hansenula polymorpha].

Mol Gen Mikrobiol Virusol 1991 Jul(7):25-8

A gene for imidazole glycerophosphate dehydratase (HIS) has been selected from the library of Hansenula polymorpha genes by complementation of Escherichia coli hisB mutations or his3 mutation of Saccharomyces cerevisiae. Inactivation of the gene in Hansenula polymorpha strain 48V, as well as Leu(-)-Leu+ conversion of phenotype and arising of antibiotic G418 resistance resulted from transformation of the strain by the linear DNA molecules of the cloned HIS-gene with the in vitro inserted LEU2 gene from Saccharomices cerevisiae and KmR gene. The Southern hybridization analysis of the Leuf+His- G418R clones representing 1-2% of transformants population together with the DNA analysis of the monospore clones from the hybrid Leu+His-G418R transformant tetrad and tester strain have revealed the locus specific nature of the clones.
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July 1991