Publications by authors named "K Christopher Garcia"

647 Publications

Implementation Science Strategies Promote Fidelity in The Food, Feeding, and Your Family Study.

J Nutr Educ Behav 2021 Jul 21. Epub 2021 Jul 21.

US Department of Agriculture, Agricultural Research Service, Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX.

Objective: Use of implementation science strategies to promote fidelity in the Food, Feeding, and Your Family study.

Design: Cluster randomized controlled trial with 3 conditions: control, in-class, or online, delivered in English or Spanish. Observations of 20% of classes.

Setting: Expanded Food and Nutrition Education Program (EFNEP) in 2 states.

Participants: EFNEP peer educators (n = 11).

Intervention: Parental feeding content incorporated into EFNEP lessons (in-class) or through text with links to videos/activities (online). Extensive educator training, scripted curriculum, frequent feedback.

Analysis: Assessment of fidelity compliance. Qualitative analysis of verbatim educator interviews and classroom observer comments.

Results: During 128 class observations (40-45 per condition), peer educators followed scripted lesson plan 78% to 89% of the time. There was no evidence of cross-contamination of parental feeding content in control and only minor sharing in online conditions. Variations with fidelity were primarily tied to the EFNEP curriculum, not the parent feeding content. Educators (n = 7) expressed favorable opinions about the Food, Feeding, and Your Family study, thought it provided valuable information, and appreciated support from EFNEP leadership.

Conclusions And Implications: Incorporating implementation science strategies can help ensure successful adherence to research protocols. With proper training and support, EFNEP peer educators can deliver an evidence-based curriculum as part of a complex research study.
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http://dx.doi.org/10.1016/j.jneb.2021.06.001DOI Listing
July 2021

Accurate prediction of protein structures and interactions using a three-track neural network.

Science 2021 Jul 15. Epub 2021 Jul 15.

Molecular Biophysics & Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.

DeepMind presented remarkably accurate predictions at the recent CASP14 protein structure prediction assessment conference. We explored network architectures incorporating related ideas and obtained the best performance with a three-track network in which information at the 1D sequence level, the 2D distance map level, and the 3D coordinate level is successively transformed and integrated. The three-track network produces structure predictions with accuracies approaching those of DeepMind in CASP14, enables the rapid solution of challenging X-ray crystallography and cryo-EM structure modeling problems, and provides insights into the functions of proteins of currently unknown structure. The network also enables rapid generation of accurate protein-protein complex models from sequence information alone, short circuiting traditional approaches which require modeling of individual subunits followed by docking. We make the method available to the scientific community to speed biological research.
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http://dx.doi.org/10.1126/science.abj8754DOI Listing
July 2021

Tissue Inhibitor of Metalloproteinase-1 Is Increased in Chagasic Cardiomyopathy.

Am J Trop Med Hyg 2021 Jul 19. Epub 2021 Jul 19.

Postgraduate Program in Health Sciences, Federal University of Bahia, Salvador, Brazil.

Chagas disease (CD) mainly conveys stroke risk through structural cardiac disease. However, stroke and cognitive impairment are seen in CD independently of cardiac disease severity. Chronic inflammation may be an explanation for this association, because inflammation plays an important role in the pathogenesis of acute ischemic stroke and dementia. In the present study, we selected five candidate biomarkers for Chagas disease: interleukin-6, membrane metalloproteinase-9, tissue inhibitor of metalloproteinase-1 (TIMP1), orosomucoid, and neprilysin. We sought to determine if mean levels of proinflammatory biomarkers are higher in patients with heart failure (HF) associated with Chagas disease when compared with other etiologies of HF. Patients were consecutively enrolled from subspecialty HF outpatient clinics at two university-based hospitals. Serum biomarker levels from blood samples were analyzed by ELISA. Severity of HF on echocardiography was worse in non-CD when compared with CD patients. No significant difference was observed in the levels of candidate biomarkers between the CD and non-CD groups. We found a significantly 2.2 ng/mL higher level of TIMP1 in CD when compared with non-CD patients with HF after adjustment for age and gender (95% confidence interval = 0.1 to 4.5, P = 0.037). In patients with heart failure, serum TIMP1 is increased in Chagas patients despite a lower myocardial disease severity on echocardiography when compared with non-Chagas patients. TIMP1 is probably one of multiple mediators of inflammatory injury.
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http://dx.doi.org/10.4269/ajtmh.20-0401DOI Listing
July 2021

Genetic mapping and phenotypic analysis of in .

MicroPubl Biol 2021 13;2021. Epub 2021 Jul 13.

Illinois State University, Normal, IL USA.

Genetic screens are used to identify genes involved in specific biological processes. An EMS mutagenesis screen in identified growth control phenotypes in the developing eye. One mutant line from this screen, , was phenotypically characterized using the FLP/FRT system and genetically mapped by complementation analysis and genomic sequencing by undergraduate students participating in the multi-institution Fly-CURE consortium. was found to have a nonsense mutation in (), anortholog of the mammalian spectraplakin (). and are involved in cytoskeletal organization and play roles during cell growth and proliferation.
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http://dx.doi.org/10.17912/micropub.biology.000418DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278230PMC
July 2021

Selective targeting of ligand-dependent and -independent signaling by GPCR conformation-specific anti-US28 intrabodies.

Nat Commun 2021 07 16;12(1):4357. Epub 2021 Jul 16.

Amsterdam Institute of Molecular and Life Sciences (AIMMS), Division of Medicinal Chemistry, Faculty of Sciences, VU University, Amsterdam, The Netherlands.

While various GPCRs, including US28, display constitutive, ligand-independent activity, it remains to be established whether ligand-dependent and -independent active conformations differ and can be selectively modulated. Previously, the agonist-bound conformation of US28 was stabilized and its structure was solved using the anti-US28 nanobody Nb7. Here we report the recognition of the constitutively active, apo-conformation of US28 by another nanobody VUN103. While the Nb7 intrabody selectively inhibits ligand-induced signaling, the VUN103 intrabody blocks constitutive signaling, indicating the existence of distinct US28 conformational states. By displacing Gα protein, VUN103 prevents US28 signaling and reduces tumor spheroids growth. Overall, nanobodies specific for distinct GPCR conformational states, i.e. apo- and agonist-bound, can selectively target and discern functional consequences of ligand-dependent versus independent signaling.
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http://dx.doi.org/10.1038/s41467-021-24574-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8285524PMC
July 2021
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