Dr. Justen Manasa, HBMLS, MPhil, PhD - University of Zimbabwe - Senior Lecturer

Dr. Justen Manasa

HBMLS, MPhil, PhD

University of Zimbabwe

Senior Lecturer

Harare, Harare | Zimbabwe

Additional Specialties: Molecular Biology, Virology, Bioinformatics

ORCID logohttps://orcid.org/0000-0002-8874-907X

Dr. Justen Manasa, HBMLS, MPhil, PhD - University of Zimbabwe - Senior Lecturer

Dr. Justen Manasa

HBMLS, MPhil, PhD

Introduction

Primary Affiliation: University of Zimbabwe - Harare, Harare , Zimbabwe

Additional Specialties:

Research Interests:


View Dr. Justen Manasa’s Resume / CV

Education

Jan 2010 - Dec 2013
University of KwaZulu-Natal
PhD
Virology
Apr 2006 - Sep 2009
University of Zimbabwe
MPhil
Immunology
Aug 1999 - Jul 2003
University of Zimbabwe
Medical laboratory sciences (Honors)
Medical Laboratory Sciences

Experience

Oct 2017 - Oct 2017
University of Zimbabwe
Lecturer
Medical Microbiology
Jul 2015 - Sep 2017
Stanford University School of Medicine
Postdoc
Infectious Diseases
Jan 2017 - Jan 2017
African Institute of Biomedical Science and Technology
Research Associate
Genomics and Molecular Science
Jan 2014 - Jun 2015
University of KwaZulu-Natal
Postdoc
Africa Centre for Health and Population Studies
Sep 2008 - Nov 2009
African Institute of Biomedical Science and Technology
Associate Director
Molecular Sciences
May 2005 - Aug 2008
African Institute of Biomedical Science and Technology
Biomedical Scientist
Molecular Diagnostics
Aug 2003 - Apr 2005
Parirenyatwa Group of Hospitals
Medical Laboratory Scientist
Pathology (HIV and Immunology)

Publications

19Publications

729Reads

313Profile Views

1PubMed Central Citations

Moderate-to-High Levels of Pretreatment HIV Drug Resistance in KwaZulu-Natal Province, South Africa.

AIDS Res Hum Retroviruses 2019 Feb 7;35(2):129-138. Epub 2019 Jan 7.

1 KwaZulu-Natal Research Innovation and Sequencing Platform (KRISP), School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, South Africa.

Introduction: There is evidence of increasing levels of pre-treatment HIV drug resistance (PDR) in Southern Africa. We used data from two large population-based HIV surveillance studies to estimate prevalence of PDR in KwaZulu-Natal, the province with the highest HIV prevalence in South Africa.

Methods: Sanger sequencing was performed on samples obtained from a longitudinal HIV surveillance programme (study A, 2013-2014) and the HIV Incidence Provincial Surveillance System (study B, 2014-2015). Sequences were included for adult HIV positive participants (age ?15 years for study A, age 15-49 years for study B) with no documented prior exposure to ART. Overall and drug class-specific PDR was estimated using the World Health Organization 2009 surveillance drug resistance mutation (SDRM) list and phylogenetic analysis was performed to establish evidence of drug resistance transmission linkage.

Results: One thousand eight hundred and forty-five (1845) sequences were analysed (611 study A; 1234 study B). An overall PDR prevalence of 9.2% (95% confidence interval (CI): 7.0-11.7) was observed for study A and 11.0% (95% CI 8.9-13.2) for study B. In study B, the prevalence of non-nucleoside reverse-transcriptase inhibitor (NNRTI) PDR exceeded 10% for sequences collected in 2014 (10.2%, 95% CI 7.5-12.9). The most prevalent SDRMs were K103NS (7.5%), M184VI (2.4%) and V106AM (1.4%). There was no evidence of large transmission chains of drug-resistant virus.

Conclusion: High level NNRTI-PDR (>10%) suggests a need to modify the standard first-line ART regimen and to focus attention on improving the quality of HIV prevention, treatment and care.

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https://www.liebertpub.com/doi/10.1089/AID.2018.0202
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http://dx.doi.org/10.1089/AID.2018.0202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360398PMC
February 2019
17 Reads
2.325 Impact Factor

Selection analyses of paired HIV-1 gag and gp41 sequences obtained before and after antiretroviral therapy.

Sci Data 2018 07 24;5:180147. Epub 2018 Jul 24.

Division of Infectious Diseases, Department of Medicine Stanford University, Stanford, CA 94305, USA.

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http://dx.doi.org/10.1038/sdata.2018.147DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6057438PMC
July 2018
16 Reads

Community Based Antiretroviral Treatment in Rural Zimbabwe.

AIDS Res Hum Retroviruses 2017 12 26;33(12):1185-1191. Epub 2017 Oct 26.

5 African Institute of Biomedical Science and Technology , Harare, Zimbabwe .

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http://www.liebertpub.com/doi/10.1089/aid.2017.0029
Publisher Site
http://dx.doi.org/10.1089/aid.2017.0029DOI Listing
December 2017
16 Reads
2.325 Impact Factor

Evolution of gag and gp41 in Patients Receiving Ritonavir-Boosted Protease Inhibitors.

Sci Rep 2017 Sep 14;7(1):11559. Epub 2017 Sep 14.

Division of Infectious Diseases, Department of Medicine Stanford University, Stanford, CA, USA.

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http://dx.doi.org/10.1038/s41598-017-11893-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5599673PMC
September 2017
91 Reads
1 Citation
5.078 Impact Factor

HIV drug resistance testing among patients failing second line antiretroviral therapy. Comparison of in-house and commercial sequencing.

J Virol Methods 2017 05 25;243:151-157. Epub 2016 Nov 25.

Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA. Electronic address:

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http://dx.doi.org/10.1016/j.jviromet.2016.11.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5393912PMC
May 2017
18 Reads
1.883 Impact Factor

Increasing HIV-1 Drug Resistance Between 2010 and 2012 in Adults Participating in Population-Based HIV Surveillance in Rural KwaZulu-Natal, South Africa.

AIDS Res Hum Retroviruses 2016 08 20;32(8):763-9. Epub 2016 Apr 20.

1 Africa Centre for Population Health, University of KwaZulu-Natal , Mtubatuba, KwaZulu-Natal, South Africa .

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http://dx.doi.org/10.1089/AID.2015.0225DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971422PMC
August 2016
15 Reads
2.325 Impact Factor

Editorial Commentary: Scaling Up Antiretroviral Therapy in Africa: Are We There Yet?

Clin Infect Dis 2016 Feb 10;62(4):519-20. Epub 2015 Nov 10.

Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, California.

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http://dx.doi.org/10.1093/cid/civ931DOI Listing
February 2016
12 Reads
8.886 Impact Factor

Southern African Treatment Resistance Network (SATuRN) RegaDB HIV drug resistance and clinical management database: supporting patient management, surveillance and research in southern Africa.

Database (Oxford) 2014 6;2014:bat082. Epub 2014 Feb 6.

Africa Centre for Health and Population Studies, School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Mtubatuba, South Africa, Department of Clinical Research, London School of Hygiene and Tropical Medicine, London, UK, Immunology department, University of Pretoria, Pretoria, South Africa, Infectious Diseases, Internal Medicine, University of the Free State School of Medicine, Bloemfontein, South Africa and Division of Medical Virology, Stellenbosch University, Stellenbosch, South Africa, National Health Laboratory Service Tygerberg, Cape Town, South Africa, Department of Virology, National Health Laboratory Service, University of KwaZulu-Natal, Durban, South Africa, The Brighton Doctoral College, Brighton and Sussex Medical School, UK, Academic Unit of primary Care and Population Science, Division of Social Statistics and Geography, University of Southampton, Southampton, UK, Jembi Health Systems, Cape Town, South Africa, School of Mathematics, Statistics and Computer Science, University of KwaZulu-Natal, Westville, South Africa and Research Department of Infection, University College of London (UCL), London, UK.

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http://dx.doi.org/10.1093/database/bat082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5630899PMC
August 2014
18 Reads
3.372 Impact Factor

An affordable HIV-1 drug resistance monitoring method for resource limited settings.

J Vis Exp 2014 Mar 30(85). Epub 2014 Mar 30.

Africa Centre for Health and Population Studies, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa;

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http://dx.doi.org/10.3791/51242DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4024245PMC
March 2014
292 Reads

Implementing antiretroviral resistance testing in a primary health care HIV treatment programme in rural KwaZulu-Natal, South Africa: early experiences, achievements and challenges.

BMC Health Serv Res 2014 Mar 7;14:116. Epub 2014 Mar 7.

Africa Centre for Health and Population Studies, University of KwaZulu-Natal, PO Box 198, Mtubatuba, KwaZulu-Natal 3935, South Africa.

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http://dx.doi.org/10.1186/1472-6963-14-116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3973961PMC
March 2014
10 Reads
1.659 Impact Factor

Drug resistance in children at virological failure in a rural KwaZulu-Natal, South Africa, cohort.

AIDS Res Ther 2014 Jan 20;11(1). Epub 2014 Jan 20.

Africa Centre for Health and Population Studies, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.

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http://dx.doi.org/10.1186/1742-6405-11-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3922737PMC
January 2014
3 Reads
1.841 Impact Factor

High level of HIV-2 false positivity in KwaZulu-Natal province: a region of South Africa with a very high HIV-1 subtype C prevalence.

J Med Virol 2013 Dec 19;85(12):2065-71. Epub 2013 Aug 19.

Department of Virology, National Health Laboratory Service, University of KwaZulu-Natal, Durban, South Africa.

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http://dx.doi.org/10.1002/jmv.23716DOI Listing
December 2013
7 Reads
2.347 Impact Factor

Primary drug resistance in South Africa: data from 10 years of surveys.

AIDS Res Hum Retroviruses 2012 Jun 12;28(6):558-65. Epub 2012 Mar 12.

Africa Centre for Health and Population Studies, Doris Duke Medical Research Institute, Nelson Mandela School of Medicine, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.

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http://dx.doi.org/10.1089/aid.2011.0284DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3358100PMC
June 2012
4 Reads
2.325 Impact Factor

Evolution and molecular epidemiology of subtype C HIV-1 in Zimbabwe.

AIDS 2009 Nov;23(18):2523-32

Stanford University School of Medicine, Stanford University Medical Center, Stanford, CA 94305, USA.

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http://dx.doi.org/10.1097/QAD.0b013e3283320ef3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923658PMC
November 2009
8 Reads
5.554 Impact Factor

Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus-infected patients in Zimbabwe.

Clin Vaccine Immunol 2007 Mar 31;14(3):293-8. Epub 2007 Jan 31.

Molecular Diagnostics Unit, African Institute of Biomedical Science and Technology, P.O. Box 2294, Harare, Zimbabwe.

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http://dx.doi.org/10.1128/CVI.00416-06DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828850PMC
March 2007
3 Reads

Top co-authors

Tulio de Oliveira
Tulio de Oliveira

University College London

10
David Katzenstein
David Katzenstein

Stanford University

7
Siva Danaviah
Siva Danaviah

University of KwaZulu-Natal

4
Sureshnee Pillay
Sureshnee Pillay

University of KwaZulu-Natal

4
Richard J Lessells
Richard J Lessells

University of KwaZulu-Natal

4
Robert W Shafer
Robert W Shafer

Stanford University

3
Soo-Yon Rhee
Soo-Yon Rhee

Stanford University

3
Andrew Skingsley
Andrew Skingsley

University of KwaZulu-Natal

3
Deenan Pillay
Deenan Pillay

University College London

3