Publications by authors named "Junyi Yu"

19 Publications

  • Page 1 of 1

Screening and prioritizing substances in groundwater in the Beijing-Tianjin-Hebei region of the North China Plain based on exposure and hazard assessments.

J Hazard Mater 2021 Sep 11;423(Pt B):127142. Epub 2021 Sep 11.

Shanghai Key Laboratory of Chemical Assessment and Sustainability, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.

Screening and prioritizing hazardous substances in groundwater is crucial to monitor and control groundwater quality. Total of 283 substances were determined in 213 groundwater samples from the Beijing-Tianjin-Hebei region during 2019-2020. 184 substances were screened as candidates. 22 prioritizing indicators were evaluated and scored for the candidates to reflect their occurrence, mobility, persistence, bioaccumulation, acute and chronic ecotoxicities with different trophic levels, and long-term human health effects. Multi-attribute decision-making technologies were applied to prioritize these candidates, including analytic hierarchy process (AHP), TOPSIS and VIKOR. Greater weightings in AHP were assigned to attributes of occurrence and acute toxicity by experts' judgment. Hierarchical cluster analysis and principal component analysis were used to transform initial matrix with the 22 indicators into an orthogonalized matrix with 6 principal components, which represented general toxicity to aquatic organism and mammal, bioaccumulation, carcinogenicity & mutagenicity, persistence, and teratogenicity & endocrine, respectively. VIKOR and TOPSIS results were similar, but different from the AHP ranking. Two filter criteria harmonized their difference. Twenty-three substances were proposed as the priority substance with high hazard and potential exposure, and nitrate-nitrogen and ammonia-nitrogen were selected as additional priority substance frequently and extensively exceeding official groundwater quality standard on the regional scale.
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http://dx.doi.org/10.1016/j.jhazmat.2021.127142DOI Listing
September 2021

Rapid postmortem ventilation improves donor lung viability by extending the tolerable warm ischemic time after cardiac death in mice.

Am J Physiol Lung Cell Mol Physiol 2021 Oct 28;321(4):L653-L662. Epub 2021 Jul 28.

Division of Pulmonary, Allergy, and Critical Care Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania.

Uncontrolled donation after cardiac death (uDCD) contributes little to ameliorating donor lung shortage due to rapidly progressive warm ischemia after circulatory arrest. Here, we demonstrated that nonhypoxia improves donor lung viability in a novel uDCD lung transplant model undergoing rapid ventilation after cardiac death and compared the evolution of ischemia-reperfusion injury to mice that underwent pulmonary artery ligation (PAL). The tolerable warm ischemia time at 37°C was initially determined in mice using a modified PAL model. The donor lung following PAL was also transplanted into syngeneic mice and compared with those that underwent rapid ventilation or no ventilation at 37°C before transplantation. Twenty-four hours following reperfusion, lung histology, [Formula: see text]/[Formula: see text] ratio, and inflammatory mediators were measured. Four hours of PAL had little impact on [Formula: see text]/[Formula: see text] ratio and acute lung injury score in contrast to significant injury induced by 5 h of PAL. Four-hour PAL lungs showed an early myeloid-dominant inflammatory signature when compared with naïve lungs and substantially injured 5 h PAL lungs. In the context of transplantation, unventilated donor lungs showed severe injury after reperfusion, whereas ventilated donor lungs showed minimal changes in [Formula: see text]/[Formula: see text] ratio, histologic score, and expression of inflammatory markers. Taken together, the tolerable warm ischemia time of murine lungs at 37°C can be extended by maintaining alveolar ventilation for up to 4 h. Nonhypoxic lung undergoing warm ischemia-reperfusion injury shows an early transcriptional signature of myeloid cell recruitment and extracellular matrix proteolysis before blood-gas barrier dysfunction and significant tissue damage.
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http://dx.doi.org/10.1152/ajplung.00011.2021DOI Listing
October 2021

Novel Design of the Chimeric Deep Inferior Epigastric Artery Perforator Flap that Provides for Three-Dimensional Reconstruction of Composite Tissue Defects of the Heel in Children.

Orthop Surg 2021 Feb 15;13(1):216-224. Epub 2021 Jan 15.

Department of Hand and Microsurgery, Department of Orthopaedics, Xiangya Hospital, Central South University, Changsha, China.

Objective: The aim of the present study was to report a novel design of the chimeric deep inferior epigastric artery perforator flap (DIEP) to achieve dead space filling, Achilles tendon bridging, and skin resurfacing simultaneously with minimal donor-site morbidity.

Methods: From September 2012 to May 2016, a retrospective study was carried out on six pediatric patients with composite soft tissue defects of the heel that were repaired with the chimeric DIEP flap. The chimeric flap design included a flap of the anterior sheath of the rectus, a block of rectus muscle, and a large skin paddle. All the parts were supplied by a common artery. After harvesting the flap, all element parts were inserted at the corresponding sites in a tension-free manner. With one set of vessel anastomoses at the recipient site, accurate repair with tendon reconstruction, dead space elimination, and wound covering were accomplished. The donor site incisions were closed initially. Data on patient age, medical history, injury severity, defect size, flap dimensions, recipient vessels, donor site closure, complications, and follow-up were collected and reviewed.

Results: Five of the six chimeric DIEP flaps survived without complications. The remaining one case experienced partial necrosis of the skin paddle caused by venous congestion, which healed after routine dressing changes. Primary donor site closure was accomplished in all cases. The mean follow-up was 18.6 months (range, 10-36 months). Five patients had satisfactory aesthetic and functional outcomes; one patient needed a secondary debulking procedure. Compared to the unaffected side, the affected side showed no obvious difference for ankle movement, tiptoe function, and patient gait during the follow-up period. Good ankle function was observed in all patients. There was no donor site breakdown, with only a slightly noticeable linear scar.

Conclusion: The chimeric DIEP flap reduced the operative time, solved the problem of deficiency of recipient vessels, and attained satisfactory functional and aesthetic outcomes with low donor site morbidity. Therefore, it is a promising option for three-dimensional reconstruction of composite defects with dead space and Achilles tendon defects as well as skin loss in children.
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http://dx.doi.org/10.1111/os.12887DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862167PMC
February 2021

Long Noncoding RNA Protects Against Cardiac Hypertrophy Through SUZ12 (Suppressor of Zeste 12 Protein Homolog)-Mediated Downregulation of MEF2A (Myocyte Enhancer Factor 2A).

Circ Heart Fail 2020 01 20;13(1):e006525. Epub 2020 Jan 20.

Department of Cardiology, Chongqing Institute of Cardiology, Chongqing Cardiovascular Clinical Research Center, Daping Hospital, The Third Military Medical University, P.R. China (J.Y., Y.Y., Z.X., C.L., C.C., C.L., Z.C., C.Y., X.X., Q.L., C.Z., G.W.).

Background: Long noncoding RNA (lncRNA) can regulate various physiological and pathological processes through multiple molecular mechanisms in cis and in trans. However, the role of lncRNAs in cardiac hypertrophy is yet to be fully elucidated.

Methods: A mouse lncRNA microarray was used to identify differentially expressed lncRNAs in the mouse hearts following transverse aortic constriction-induced pressure overload comparing to the sham-operated samples. The direct impact of one lncRNA, on cardiomyocyte hypertrophy was characterized in neonatal rat cardiomyocytes in response to phenylephrine by targeted knockdown and overexpression. The in vivo function of was analyzed in mouse hearts by using cardiac-specific adeno-associated virus, serotype 9-short hairpin RNA to knockdown in combination with transverse aortic constriction. Using catRAPID program, an interaction between and SUZ12 (suppressor of zeste 12 protein homolog) was predicted and validated by RNA immunoprecipitation and immunoblotting following RNA pull-down. Chromatin immunoprecipitation was performed to determine SUZ12 or H3K27me3 occupancy on the MEF2A (myocyte enhancer factor 2A) promoter. Finally, the expression of human (leukemia-associated noncoding IGF1R activator RNA 1 [LUNAR1]) in the serum samples from patients of hypertrophic cardiomyopathy was tested by quantitative real-time polymerase chain reaction.

Results: A previously unannotated lncRNA, antihypertrophic interrelated transcript (), was identified to be upregulated in the mouse hearts after transverse aortic constriction. Inhibition of induced cardiac hypertrophy, both in vitro and in vivo, associated with increased expression of MEF2A, a critical transcriptional factor involved in cardiac hypertrophy. In contrast, overexpression of significantly attenuated stress-induced cardiac hypertrophy in vitro. Furthermore, was significantly upregulated in serum samples of patients diagnosed with hypertensive heart disease versus nonhypertrophic hearts (1.46±0.17 fold, =0.0325). Mechanistically, directly bound and recruited SUZ12, a core PRC2 (polycomb repressive complex 2) protein, to the promoter of MEF2A, triggering its trimethylation on H3 lysine 27 (H3K27me3) residues and mediating the downregulation of MEF2A, thereby preventing cardiac hypertrophy.

Conclusions: is a lncRNA with a significant role in cardiac hypertrophy regulation through epigenomic modulation. is a potential therapeutic target of cardiac hypertrophy.
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http://dx.doi.org/10.1161/CIRCHEARTFAILURE.119.006525DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241255PMC
January 2020

SODs involved in the hormone mediated regulation of HO content in Kandelia obovata root tissues under cadmium stress.

Environ Pollut 2020 Jan 20;256:113272. Epub 2019 Sep 20.

Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, Xiamen University, Xiamen, 361102, PR China; State Key Laboratory of Marine Environmental Science, XiamenUniversity, Xiamen, 361102, PR China. Electronic address:

Cadmium (Cd) pollution in mangrove wetlands has received increasing attention as urbanization expands rapidly. As a dominant mangrove species, Kandelia obovata is highly tolerant to Cd toxicity. Plant hormones and superoxide dismutase (SODs) play critical roles in the response to heavy metal stress in K. obovata roots. Although theirs important influence have been reported, the regulation mechanism between SODs and plant hormones in Cd detoxification by K. obovata roots remains limited. Here, we investigated relationships among SOD, plant hormones, and Cd tolerance in K. obovata roots exposed to Cd. We found that Cd was retained in the epidermis and exodermis of roots, and the epidermis and exodermis had highest hydrogen peroxide (HO) content and SOD activity. Similarly, SOD isozymes also exhibited distinct activity in the different parts of root. Overexpressed KoCSD3 and KoFSD2 individually in Nicotiana benthamiana revealed that different SOD members contributed to HO content regulation by promote the activity of downstream antioxidant enzymes under Cd treatment. In addition, assays on the effects of hormones showed that increased endogenous indole-3-acetic acid (IAA) was observed in the cortex and stele, whereas the abscisic acid (ABA) content was enhanced in the epidermis and exodermis in roots during Cd treatment. The results of exogenous hormones treatment indicated that KoFSD2 upregulated under ABA and IAA treatment, but KoCSD3 only induced by ABA stimulation. Taken together, our results reveal the relationship between SODs and plant hormones, which expands the knowledge base regarding KoSODs response to plant hormones and mediating HO concentration under Cd stress.
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http://dx.doi.org/10.1016/j.envpol.2019.113272DOI Listing
January 2020

Analysis of anatomical changes and cadmium distribution in Aegiceras corniculatum (L.) Blanco roots under cadmium stress.

Mar Pollut Bull 2019 Dec 5;149:110536. Epub 2019 Sep 5.

State Key Lab of Marine Environmental Science, Xiamen University, Xiamen 361102, China. Electronic address:

Heavy metal stress changes the morphological and anatomical structure of plant organs. In this study, we determined the anatomical changes and Cd distribution in the roots of Aegiceras corniculatum (L.) Blanco (Black mangrove) under Cd stress. The results showed that Cd levels in A. corniculatum root tissues decreased in the following order: endodermis > pith > xylem > epidermis and exodermis > phloem > cortex. The endodermis secondary casparian strip replaces exodermis casparian strip and plays a role in the "retardation mechanism", which sort of compensates for the missing exodermis retardation effect. The xylem and pith both show high affinity for Cd and contain enriched Cd. This creates a low-Cd environment for phloem and protects the nutrient transport function of the vasculature against Cd toxicity. The present study provides new evidences suggesting that Cd regional enrichment and anatomical structure changes are an adaptive strategy of mangrove plants to HM tolerance.
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http://dx.doi.org/10.1016/j.marpolbul.2019.110536DOI Listing
December 2019

Regulation of Cholesterol Homeostasis by a Novel Long Non-coding RNA LASER.

Sci Rep 2019 05 22;9(1):7693. Epub 2019 May 22.

Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, P.R. China.

Genome-wide association studies (GWAS) have identified many genetic variants in genes related to lipid metabolism. However, how these variations affect lipid levels remains elusive. Long non-coding RNAs (lncRNAs) have been implicated in a variety of biological processes. We hypothesize lncRNAs are likely to be located within disease or trait-associated DNA regions to regulate lipid metabolism. The aim of this study was to investigate whether and how lncRNAs in lipid- associated DNA regions regulate cholesterol homeostasis in hepatocytes. In this study, we identified a novel long non-coding RNA in Lipid Associated Single nucleotide polymorphism gEne Region (LASER) by bioinformatic analysis. We report that LASER is highly expressed in both hepatocytes and peripheral mononuclear cells (PBMCs). Clinical studies showed that LASER expression is positively related with that of cholesterol containing apolipoprotein levels. In particular, we found that LASER is positively correlated with plasma PCSK9 levels in statin free patients. siRNAs mediated knock down of LASER dramatically reduces intracellular cholesterol levels and affects the expression of genes involved in cholesterol metabolism. Transcriptome analyses show that knockdown of LASER affects the expression of genes involved in metabolism pathways. We found that HNF-1α and PCSK9 were reduced after LASER knock-down. Interestingly, the reduction of PCSK9 can be blocked by the treatment of berberine, a natural cholesterol-lowering compound which functions as a HNF-1α antagonist. Mechanistically, we found that LASER binds to LSD1 (lysine-specific demethylase 1), a member of CoREST/REST complex, in nucleus. LASER knock-down enhance LSD1 targeting to genomic loci, resulting in decreased histone H3 lysine 4 mono-methylation at the promoter regions of HNF-1α gene. Conversely, LSD1 knock-down abolished the effect of LASER on HNF-1α and PCSK9 expressions. Finally, we found that statin treatment increased LASER expression, accompanied with increased PCSK9 expression, suggesting a feedback regulation of cholesterol on LASER expression. This observation may partly explain the statin escape during anti-cholesterol treatment. These findings identified a novel lncRNA in cholesterol homeostasis. Therapeutic targeting LASER might be an effective approach to augment the effect of statins on cholesterol levels in clinics.
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http://dx.doi.org/10.1038/s41598-019-44195-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6531449PMC
May 2019

Glucagon Receptor Antagonism Ameliorates Progression of Heart Failure.

JACC Basic Transl Sci 2019 Apr 13;4(2):161-172. Epub 2019 Mar 13.

Department of Anesthesiology, Cardiovascular Research Laboratories, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California.

Mice were treated with a fully human monoclonal glucagon receptor antagonistic antibody REMD2.59 following myocardial infarction or pressure overload. REMD2.59 treatment blunted cardiac hypertrophy and fibrotic remodeling, and attenuated contractile dysfunction at 4 weeks after myocardial infarction. In addition, REMD2.59 treatment at the onset of pressure overload significantly suppressed cardiac hypertrophy and chamber dilation with marked preservation of cardiac systolic and diastolic function. Initiation of REMD2.59 treatment 2 weeks after pressure overload significantly blunted the progression of cardiac pathology. These results provide the first in vivo proof-of-concept evidence that glucagon receptor antagonism is a potentially efficacious therapy to ameliorate both onset and progression of heart failure.
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http://dx.doi.org/10.1016/j.jacbts.2018.11.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6488764PMC
April 2019

Epigenetics in dilated cardiomyopathy.

Curr Opin Cardiol 2019 05;34(3):260-269

Departments of Anesthesiology, Physiology and Medicine, David Geffen School of Medicine, University of California at Los Angeles, California, USA.

Purpose Of Review: Characterized by enlarged ventricle and loss of systolic function, dilated cardiomyopathy (DCM) has the highest morbidity among all the cardiomyopathies. Although it is well established that DCM is typically caused by mutations in a large number of genes, there is an emerging appreciation for the contribution of epigenetic alteration in the development of DCM.

Recent Findings: We present some of the recent progress in the field of epigenetics in DCM by focusing on the four major epigenetic modifications, that is, DNA methylation, histone modification, chromatin remodeling as well as the noncoding RNAs. The major players involved in these DCM-related epigenetic reprogramming will be highlighted. Finally, the diagnostic and the therapeutic implications for DCM based on new knowledge of epigenetic regulation will also be discussed.

Summary: As a rapidly expanding field, epigenetic studies in DCM have the promise to yield both novel mechanistic insights as well as potential new avenues for more effective treatment of the disease.
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http://dx.doi.org/10.1097/HCO.0000000000000616DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6581652PMC
May 2019

Prolonged Cold Ischemia Induces Necroptotic Cell Death in Ischemia-Reperfusion Injury and Contributes to Primary Graft Dysfunction after Lung Transplantation.

Am J Respir Cell Mol Biol 2019 08;61(2):244-256

1Division of Pulmonary, Allergy, and Critical Care Medicine, Department of Medicine.

Primary graft dysfunction (PGD) is a major cause of morbidity and mortality after lung transplantation. Ischemia-reperfusion injury (IRI) is a key event that contributes to PGD, though complex interactions affect donor lungs status, such as preceding brain death (BD), hemorrhagic shock (HS), and pre-engraftment lung management, the latter recognized as important risk factors for PGD. We hypothesized that a multi-hit isogenic mouse model of lung transplantation is more closely linked to PGD than IRI alone. Left lung transplants were performed between inbred C57BL/6 mice. A one-hit model of IRI was established by inducing cold ischemia (CI) of the donor lungs at 0°C for 1, 72, or 96 hours before engraftment. Multi-hit models were established by inducing 24 hours of HS and/or 3 hours of BD before 24 hours of CI. The recipients were killed at 24 hours after transplant and lung graft samples were analyzed. In the one-hit model of IRI, up to 72-hour CI time resulted in minimal cellular infiltration near small arteries after 24-hour reperfusion. Extension of CI time to 96 hours led to increased cellular infiltration and necroptotic pathway activation, without evidence of apoptosis, after 24-hour reperfusion. In a multi-hit model of PGD, "HS + BD + IRI" demonstrated increased lung injury, cellular infiltration, and activation of necroptotic and apoptotic pathways compared with IRI alone. Treatment with an inhibitor of receptor-interacting protein kinase 1 kinase, necrostatin-1, resulted in a significant decrease of downstream necroptotic pathway activation in both single- and multi-hit models of IRI. Thus, activation of necroptosis is a central event in IRI after prolonged CI, though it may not be sufficient to cause PGD alone. Pathological evaluation of donor lungs after CI-induced IRI, in conjunction with pre-engraftment donor lung factors in our multi-hit model, demonstrated early evidence of lung injury consistent with PGD. Our findings support the premise that pre-existing donor lung status is more important than CI time alone for inflammatory pathway activation in PGD, which may have important clinical implications for donor lung retrieval.
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http://dx.doi.org/10.1165/rcmb.2018-0207OCDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6670033PMC
August 2019

Humanin analog enhances the protective effect of dexrazoxane against doxorubicin-induced cardiotoxicity.

Am J Physiol Heart Circ Physiol 2018 09 18;315(3):H634-H643. Epub 2018 May 18.

Division of Endocrinology, Department of Medicine, Los Angeles Biomedical Research Institute and Harbor-UCLA Medical Center, University of California-Los Angeles, Torrance, California.

The chemotherapeutic effect of doxorubicin (Dox) is limited by cumulative dose-dependent cardiotoxicity in cancer survivors. Dexrazoxane (DRZ) is approved to prevent Dox-induced cardiotoxicity. Humanin and its synthetic analog HNG have a cytoprotective effect on the heart. To investigate the cardioprotective efficacy of HNG alone or in combination with DRZ against Dox-induced cardiotoxicity, 80 adult male mice were randomly divided into 8 groups to receive the following treatments via intraperitoneal injection: saline dailym HNG (5 mg/kg) daily, DRZ (60 mg/kg) weekly, Dox (3 mg/kg) weekly, DRZ + HNG, Dox + HNG, Dox + DRZ, and Dox + HNG + DRZ. Echocardiograms were performed before and at 4, 8, and 9.5 wk after the beginning of treatment. All mice were euthanized at 10 wk. In the absence of Dox, HNG, DRZ, or DRZ + HNG had no adverse effect on the heart. Dox treatment caused decreases in ejection fraction and cardiac mass and increases in cardiomyocyte apoptosis and intracardiac fibrosis. HNG or DRZ alone blunted the Dox-induced decrease in left ventricle posterior wall thickness and modestly ameliorated the Dox-induced decrease in ejection fraction. HNG + DRZ significantly ameliorated Dox-induced decreases in ejection function, cardiac fibrosis, and cardiac mass. Using a targeted analysis for the mitochondrial gene array and protein expression in heart tissues, we demonstrated that HNG + DRZ reversed DOX-induced altered transcripts that were biomarkers of cardiac damage and uncoupling protein-2. We conclude that HNG enhances the cardiac protective effect of DRZ against Dox-induced cardiotoxicity. HNG + DRZ protects mitochondria from Dox-induced cardiac damage and blunts the onset of cardiac dysfunction. Thus, HNG may be an adjuvant to DRZ in preventing Dox-induced cardiotoxicity. NEW & NOTEWORTHY Doxorubicin (Dox) is commonly used for treating a wide range of human cancers. However, cumulative dosage-dependent carditoxicity often limits its clinical applications. We demonstrated in this study that treating young adult male mice with synthetic humanin analog enhanced the cardiac protective effect of dexrazoxane against chemotherapeutic agent Dox-induced cardiac dysfunction. Thus, humanin analog can potentially serve as an adjuvant to dexrazoxane in more effectively preventing Dox-induced cardiac dysfunction and cardiomyopathy.
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http://dx.doi.org/10.1152/ajpheart.00155.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6734085PMC
September 2018

Impact of synoptic weather patterns on 24 h-average PM concentrations in the North China Plain during 2013-2017.

Sci Total Environ 2018 Jun 3;627:200-210. Epub 2018 Feb 3.

Department of Teaching and Research, China Executive Leadership Academy Pudong (CELAP), Shanghai 201204, China. Electronic address:

North China Plain area (NCP) is one of the most densely populated and heavily polluted regions in the world. In the last five years, frequently happened fine particulate matter (PM) serious pollution events were one of the top environmental concerns in China. As PM concentrations are highly influenced by synoptic flow patterns and local meteorological conditions, a two-stage hierarchical clustering method based on dynamic principal component analysis (DPCA) and standard k-means clustering algorithm was employed to classify synoptic wind fields into 6 patterns over the NCP area using the data of 5 PM seasons (Sept. 15th-Apr. 15th) from 2013 to 2017. Among the six identified synoptic patterns, pattern of uniform pressure field (U) and that of zonal high pressure (Z) accounted for 78.21%, 65.55%, 63.56%, 57.11%, 59.13% and 58.27% studied heavy smog pollution events in Beijing, Tianjin, Tangshan, Baoding, Shijiazhuang and Xingtai city. The two particular patterns were associated with uniform pressure field and sparsely latitudinal isobar in 850 hPa level, respectively. They were also characterized by high relative humidity, low temperature, low-speed northerly wind in Tianjin and Tangshan, and southerly wind in the other cities. Under the continuous control of pattern Z, the values of 24 h-average PM were found to increase at a rate of 31.78 μg/m per day. To evaluate the contribution of meteorological factors and precursors to PM levels, linear mixed-effects models (LMMs) were applied to establish relations among 24 h-average PM concentrations, concentrations of main precursors, local meteorological factors and synoptic patterns. Results show that the variations of precursors, local meteorological factors and synoptic flow patterns can explain 51.67%, 19.15% and 14.01% changes of the 24 h-average PM concentrations, respectively. This study illustrates that dense precursor emissions are still the main cause for heavy haze pollution events, although meteorological conditions play almost equal roles sometimes.
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http://dx.doi.org/10.1016/j.scitotenv.2018.01.248DOI Listing
June 2018

Distribution correlations of cadmium to calcium, phosphorus, sodium and chloridion in mangrove Aegiceras corniculatum root tissues.

Mar Pollut Bull 2018 Jan 11;126:179-183. Epub 2017 Nov 11.

Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, Xiamen University, Xiamen 361102, China.

Nutriment distributions might influence Cd distribution and Cd tolerance in mangrove plant roots. To demonstrate this, Aegiceras corniculatum was stressed by Cd, and the distributions of Cd, Ca, P, Na and Cl in plant roots were detected with the aid of SEM-EDX. It was found that endodermis, pith and xylem were the predominant tissues for retardation and regional enrichment of Cd. Na and Cl distributions suggest a critical role of salt resistance tissues on Cd tolerance in roots. P participated in Cd retardation and regional enrichment of endodermis and xylem. P, Na, Cl and Ca distribution had a high correlation to that of Cd in roots. The synergetic accumulation between Ca and Cd could be a crucial mechanism for Cd tolerance in A. corniculatum roots. In conclusion, the research of Cd and nutriment distributions in A. corniculatum roots deepens the understanding on Cd tolerance in mangrove plants.
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http://dx.doi.org/10.1016/j.marpolbul.2017.10.074DOI Listing
January 2018

Co-effect of phosphorus and cadmium interaction on fraction distribution and accumulation of phosphorus in mangrove-sediment system.

Authors:
Jingna Du Junyi Yu

Int J Phytoremediation 2017 Jul;19(7):658-661

b Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems , Xiamen University , Xiamen , China.

Mangrove [Kandelia obovata (S. L.)] seedlings were cultivated in rhizoboxes under different rates of phosphorus (P) and cadmium (Cd) level. The speciation distributions of P both in the rhizosphere and non-rhizosphere sediments were examined using sequential extraction procedures. P contents in different K. obovata (S.L.) tissues were also determined. Results showed that considerable differences existed in P speciation distribution between rhizosphere and bulk sediments. A higher proportion of iron-bound phosphate (Fe-P) was found in the rhizosphere sediments, while a relatively higher concentration of exchangeable phosphate (Ex-P) and Aluminum-bound phosphate was found in the bulk sediments. P accumulation in plant tissues was significantly positively correlated to Ex-P and Fe-P. Results indicated that root activities play an important role in the P cycling. And the coexistence of P and Cd induced higher P accumulation in mangrove plants. It is suggested that the root-induced chemical and biological changes in the rhizosphere environment play an important role in enhancing the bioavailability of soil P.
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http://dx.doi.org/10.1080/15226514.2016.1278425DOI Listing
July 2017

The effects of sulfur amendments on the geochemistry of sulfur, phosphorus and iron in the mangrove plant (Kandelia obovata (S. L.)) rhizosphere.

Mar Pollut Bull 2017 Jan 3;114(2):733-741. Epub 2016 Nov 3.

Department of Geography, Queen Mary, University of London, London E1 4NS, UK.

P (phosphorus) and Fe (iron) are limiting elements and S (sulfur) is an important element of the biogeochemical cycle in the mangrove environment. To assess the effects of sulfur on the geochemical cycling of Fe and P at the sediment-plant interface, the speciation distributions of Fe, P and S in sediments were examined. The data showed that higher proportions of amorphous Fe, Fe-bound phosphate, chromium reducible sulfur and elemental sulfur were found in the rhizosphere, while more crystalline Fe, exchangeable phosphate and acid-volatile sulfide were determined in the non-rhizosphere. Sulfate application induced an increase in the Ex-P concentration, high P accumulation and high iron plaque deposition in the roots. In conclusion, sulfate applications had a significant influence on the geochemical cycling of Fe and P in the sediments. It significantly curtailed the Fe and P limit to plant growth and enhanced plant resistance to the rugged surroundings in mangrove.
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http://dx.doi.org/10.1016/j.marpolbul.2016.10.070DOI Listing
January 2017

Circulating 'lncRNA OTTHUMT00000387022' from monocytes as a novel biomarker for coronary artery disease.

Cardiovasc Res 2016 12 7;112(3):714-724. Epub 2016 Feb 7.

Department of Cardiology, Daping Hospital, The Third Military Medical University, Chongqing, P.R. China

Aims: Long non-coding RNAs (lncRNAs) have been found to be involved in the pathogenesis of coronary artery disease (CAD). However, it remains to be established whether or not circulating lncRNAs can serve as biomarkers of CAD.

Methods And Results: Using a microarray-based lncRNA expression profiling, we found 86 lncRNAs that were differentially expressed in circulating peripheral blood monocytes and plasma from 15 CAD patients and 15 control subjects. After choosing a consistent criterion (average normalized intensity ≥7 with significance <0.005) and confirmed by quantitative PCR, only three lncRNAs (CoroMarker, BAT5, and IL21R-AS1) remained as candidate CAD biomarkers. Using the analysis of area under the curve (AUC) of the receiver-operating characteristic in another pilot group and another larger cohort, CoroMarker was found to be the best candidate biomarker for CAD with an AUC of 0.920 and 95% confidence interval of 0.892-0.947. CoroMarker was independent from known CAD risk factors and other cardiovascular diseases. In a prospective study, we found that the sensitivity and specificity of CoroMarker were 76 and 92.5%, respectively. Functional enrichment analysis showed CoroMarker to be clustered with genes positively associated with signal transduction, transmembrane transport, synaptic transmission, and innate immunity and negatively associated with inflammation. These findings were validated in THP-1 cells; CoroMarker siRNA treatment decreased the concentrations of proinflammatory cytokines [interleukin (IL)-1β, IL-6, and tumour necrosis factor α] in the culture medium.

Conclusion: The present study suggests that CoroMarker is a novel and specific biomarker of CAD.
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http://dx.doi.org/10.1093/cvr/cvw022DOI Listing
December 2016

Influence of the phenols on the biogeochemical behavior of cadmium in the mangrove sediment.

Chemosphere 2016 Feb 18;144:2206-13. Epub 2015 Nov 18.

Key Laboratory of Ministry of Education for Coastal and Wetland Ecosystems, Xiamen University, Xiamen, 361102, China; State Key Lab of Marine Evironmental Science, Xiamen University, Xiamen, 361102, China. Electronic address:

Phenols exert a great influence on the dynamic process of Cd in the soil-plant interface. We investigated the influence of phenols on the biogeochemical behavior of cadmium in the rhizosphere of Avicennia marina (Forsk) Vierh. All combinations of four levels of cadmium (0, 1, 2 and 4 mg/kg DW) and two levels of phenol (0 and 15 mg/kg DW) were included in the experimental design. We found that phenols facilitated increasing concentrations of exchangeable cadmium (Ex-Cd), acid volatile sulfide (AVS) and reactive solid-phase Fe (II) in sediments, and iron in plants, but inhibited Cd accumulation in iron plaque and roots. The concentrations of AVS and reactive solid-phase Fe (II) were significantly positively correlated with Cd treatment. As for the biogeochemical behavior of Cd in mangrove sediments, this research revealed that phenols facilitated activation and mobility of Cd. They disturbed the "source-sink" balance of Cd and turned it into a "source", whilst decreasing Cd absorption in A. marina. Additionally, phenols facilitated iron absorption in the plant and alleviated the Fe limit for mangrove plant growth.
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http://dx.doi.org/10.1016/j.chemosphere.2015.10.128DOI Listing
February 2016

Plasma long non-coding RNA, CoroMarker, a novel biomarker for diagnosis of coronary artery disease.

Clin Sci (Lond) 2015 Oct 11;129(8):675-85. Epub 2015 Jun 11.

Department of Cardiology, Daping Hospital, Third Military Medical University, Chongqing, China Chongqing Institute of Cardiology, Chongqing, China

Long non-coding RNAs (lncRNAs) have been reported to be involved in the pathogenesis of cardiovascular disease (CVD), but whether circulating lncRNAs can serve as a coronary artery disease (CAD), biomarker is not known. The present study screened lncRNAs by microarray analysis in the plasma from CAD patients and control individuals and found that 265 lncRNAs were differentially expressed. To find specific lncRNAs as possible CAD biomarker candidates, we used the following criteria for 174 up-regulated lncRNAs: signal intensity ≥8, fold change >2.5 and P<0.005. According to these criteria, five intergenic lncRNAs were identified. After validation by quantitative PCR (qPCR), one lncRNA was excluded from the candidate list. The remaining four lncRNAs were independently validated in another population of 20 CAD patients and 20 control individuals. Receiver operating characteristic (ROC) curve analysis showed that lncRNA AC100865.1 (referred to as CoroMarker) was the best of these lncRNAs. CoroMarker levels were also stable in plasma. The predictive value of CoroMarker was further assessed in a larger cohort with 221 CAD patients and 187 control individuals. Using a diagnostic model with Fisher's criteria, taking the risk factors into account, the optimal sensitivity of CoroMarker for CAD increased from 68.29% to 78.05%, whereas the specificity decreased slightly from 91.89% to 86.49%. CoroMarker was stable in plasma because it was mainly in the extracellular vesicles (EVs), probably from monocytes. We conclude that CoroMarker is a stable, sensitive and specific biomarker for CAD.
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http://dx.doi.org/10.1042/CS20150121DOI Listing
October 2015

[Update on the role of LncRNA ANRIL at human chromosome 9p21 on the pathogenesis of atherosclerosis].

Zhonghua Xin Xue Guan Bing Za Zhi 2015 Jan;43(1):82-5

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January 2015
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