Publications by authors named "Junxiang Wang"

48 Publications

Knockout of Two Cry-Binding Aminopeptidase N Isoforms Does Not Change Susceptibility of Larvae to subsp. Cry4Ba and Cry11Aa Toxins.

Insects 2021 Mar 5;12(3). Epub 2021 Mar 5.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University (FAFU), Fuzhou 350002, China.

The insecticidal Cry4Ba and Cry11Aa crystal proteins from subsp. (Bti) are highly toxic to larvae. The glycosylphosphatidylinositol (GPI)-anchored APN was identified as an important membrane-bound receptor for multiple Cry toxins in numerous Lepidoptera, Coleoptera, and Diptera insects. However, there is no direct molecular evidence to link APN of to Bti toxicity . In this study, two Cry4Ba/Cry11Aa-binding GPI-APN isoforms (APN1 and APN2) were individually knocked-out using CRISPR/Cas9 mutagenesis, and the APN1/APN2 double-mutant homozygous strain was generated using the reverse genetics approach. ELISA assays showed that the high binding affinity of Cry4Ba and Cry11Aa protoxins to the midgut brush border membrane vesicles (BBMVs) from these APN knockouts was similar to the background from the wild-type (WT) strain. Likewise, the bioassay results showed that neither the single knockout of APN1 or APN2, nor the simultaneous disruption of APN1 and APN2 resulted in significant changes in susceptibility of larvae to Cry4Ba and Cry11Aa toxins. Accordingly, our results suggest that APN1 and APN2 may not mediate Bti Cry4Ba and Cry11Aa toxicity in larvae as their binding proteins.
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http://dx.doi.org/10.3390/insects12030223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002144PMC
March 2021

Effects of physicochemical properties of Au cyanidation tailings on cyanide microbial degradation.

J Environ Sci Health A Tox Hazard Subst Environ Eng 2021 Feb 17:1-21. Epub 2021 Feb 17.

College of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao, China.

The initial cyanide (CN) concentration and amount of co-contaminants in GCTs can inhibit bacterial growth and reduce the CN-degrading ability of bacteria. Several microorganisms can biotransform a wide range of organic and inorganic industrial contaminants into nontoxic compounds. However, active enzymatic CN metabolism processes are mostly constrained by the physical and chemical characteristics of GCTs. High concentrations of toxic metal co-contaminants, such as, Pb, and Cr, and factors, such as pH, temperature, and oxygen concentration create oxidative stress and limit the CN-degrading potential of cyanotrophic strains. The effects of such external and internal factors on the CN-degrading ability of bacteria hinder the selection of suitable microorganisms for CN biodegradation. Therefore, understanding the effects of the physicochemical properties of GCTs on cyanobacteria strains can help identify suitable microbes and favorable environmental conditions to promote microbial growth and can also help design efficient CN biodegradation processes. In this review, we present a detailed analysis of the physicochemical properties of GCTs and their effects on microbial CN degradation.
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http://dx.doi.org/10.1080/10934529.2021.1885259DOI Listing
February 2021

Elevated Circulating IL-10 Producing Breg, but Not Regulatory B Cell Levels, Restrain Antibody-Mediated Rejection After Kidney Transplantation.

Front Immunol 2020 28;11:627496. Epub 2021 Jan 28.

Kidney Transplantation Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Background: Antibody-mediated rejection (AMR) occupies a major position for chronic rejection after kidney transplantation. Regulatory B cell (Breg) has been reported to have an inhibitory immune function, which contributes to the resistance for AMR.

Methods: A nested case-control study for nine healthy donors, 25 stable (ST) patients, and 18 AMR patients was performed to determine the type of Breg in maintaining immune tolerance and preventing AMR.

Results: Compared to the ST group, circulating interleukin (IL)-10 Bregs, but not Bregs, significantly decreased. The receiver operating characteristic (ROC) curve analysis revealed that rather than the circulating Bregs, decreased circulating IL-10 Breg levels were positively associated with AMR. However, kidney B cell and IL-10 infiltration was significantly increased in the AMR group with high expression of C-X-C motif chemokine 13 (CXCL13). In addition, circulating IL-10 Bregs, rather than Bregs, remained higher than those at pre-operation, during the 90-day post-operation in immune homeostasis.

Conclusion: The circulating IL-10 Breg levels are more appropriate measures for assessing the resistance of AMR after kidney transplantation.
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http://dx.doi.org/10.3389/fimmu.2020.627496DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7877339PMC
January 2021

Mapping and role of T cell response in SARS-CoV-2-infected mice.

J Exp Med 2021 04;218(4)

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.

Virus-specific T cells play essential roles in protection against multiple virus infections, including SARS-CoV and MERS-CoV. While SARS-CoV-2-specific T cells have been identified in COVID-19 patients, their role in the protection of SARS-CoV-2-infected mice is not established. Here, using mice sensitized for infection with SARS-CoV-2 by transduction with an adenovirus expressing the human receptor (Ad5-hACE2), we identified SARS-CoV-2-specific T cell epitopes recognized by CD4+ and CD8+ T cells in BALB/c and C57BL/6 mice. Virus-specific T cells were polyfunctional and were able to lyse target cells in vivo. Further, type I interferon pathway was proved to be critical for generating optimal antiviral T cell responses after SARS-CoV-2 infection. T cell vaccination alone partially protected SARS-CoV-2-infected mice from severe disease. In addition, the results demonstrated cross-reactive T cell responses between SARS-CoV and SARS-CoV-2, but not MERS-CoV, in mice. Understanding the role of the T cell response will guide immunopathogenesis studies of COVID-19 and vaccine design and validation.
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http://dx.doi.org/10.1084/jem.20202187DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814348PMC
April 2021

The negative role of histone acetylation in cobalt chloride-induced neurodegenerative damages in SHSY5Y cells.

Ecotoxicol Environ Saf 2021 Feb 28;209:111832. Epub 2020 Dec 28.

Fujian Provincial Key Laboratory of Environmental Factors and Cancer, School of Public Health, Fujian Medical University, Fuzhou 350122, China; Department of Preventive Medicine, School of Public Health, Fujian Medical University, Fuzhou 350122, China; The Key Laboratory of Environment and Health, School of Public Health, Fujian Medical University, Fuzhou 350122, China. Electronic address:

Cobalt has been known for its neurotoxicity in numerous studies. However, the molecular mechanism underlying cobalt-induced neurotoxicity remains largely unknown. In this study, two neuroblastoma (SHSY5Y and N2a) cell lines and a phaeochromocytoma (PC12) line were used as in vitro models. Cells were treated for 24 h with 50, 100, 200, 300, 400 µM cobalt chloride (CoCl) or cultured with 300 μM CoCl for 4, 8, 12 and 24 h to investigate the effects of histone acetylation on CoCl-induced neurodegenerative damages. Our findings demonstrate that CoCl suppresses the acetylation of histone H3 and H4 in a time-dependent and dosage-dependent manner. Furthermore, CoCl selectively decreases the expression and activity of histone acetyltransferase (HAT) but has no effects on histone deacetylase (HDAC) in SHSY5Y cells. More importantly, we show that 100 ng/mL HDAC inhibitor trichostatin (TSA) pre-treatment partly attenuates 300 μM CoCl-induced neurodegenerative damages in SHSY5Y cells. Mechanistic analyses show that CoCl-induced neurodegenerative damages are associated with the dysfunction of APP, BACE1, PSEN1, NEP and HIF-1α genes, whose expression are partly mediated by histone modification. In summary, we demonstrate that histone acetylation is involved in CoCl-induced neurodegenerative damages. Our study indicates an important connection between histone modification and the pathological process of neurodegenerative damages and provides a mechanism for cobalt-mediated epigenetic regulation.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111832DOI Listing
February 2021

One-pot microwave-hydrothermally synthesized carbon nanotube-cerium oxide nanocomposites for enhanced visible photodegradation of acid orange 7.

Phys Chem Chem Phys 2020 Oct;22(41):23743-23753

Integrated Composites Laboratory (ICL), Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, TN 37996, USA.

Carbon nanotubes (CNT)-cerium oxide (CeO2) nanocomposites were fabricated successfully by one-pot microwave hydrothermal growth of regular CeO2 nanoparticles with a size of 8 nm on hydroxyl-functionalized multi-walled CNTs. These nanocomposite photocatalysts demonstrated an acid orange (AO7) photocatalytic degradation efficiency of above 90% under solar-simulated light irradiation for 3 h, which was much higher than that of the pure CeO2 nanoparticles. The enhanced photocatalytic activity was observed to mainly originate from the ˙O2- and hole traps, while the hydroxyl radical ˙OH played a secondary role.
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http://dx.doi.org/10.1039/d0cp00431fDOI Listing
October 2020

T-cell responses to MERS coronavirus infection in people with occupational exposure to dromedary camels in Nigeria: an observational cohort study.

Lancet Infect Dis 2021 03 6;21(3):385-395. Epub 2020 Oct 6.

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China; Guangzhou Eighth People's Hospital of Guangzhou Medical University, Guangzhou, China. Electronic address:

Background: Middle East respiratory syndrome (MERS) remains of global public health concern. Dromedary camels are the source of zoonotic infection. Over 70% of MERS coronavirus (MERS-CoV)-infected dromedaries are found in Africa but no zoonotic disease has been reported in Africa. We aimed to understand whether individuals with exposure to dromedaries in Africa had been infected by MERS-CoV.

Methods: Workers slaughtering dromedaries in an abattoir in Kano, Nigeria, were compared with abattoir workers without direct dromedary contact, non-abattoir workers from Kano, and controls from Guangzhou, China. Exposure to dromedaries was ascertained using a questionnaire. Serum and peripheral blood mononuclear cells (PBMCs) were tested for MERS-CoV specific neutralising antibody and T-cell responses.

Findings: None of the participants from Nigeria or Guangdong were MERS-CoV seropositive. 18 (30%) of 61 abattoir workers with exposure to dromedaries, but none of 20 abattoir workers without exposure (p=0·0042), ten non-abattoir workers or 24 controls from Guangzhou (p=0·0002) had evidence of MERS-CoV-specific CD4 or CD8 T cells in PBMC. T-cell responses to other endemic human coronaviruses (229E, OC43, HKU-1, and NL-63) were observed in all groups with no association with dromedary exposure. Drinking both unpasteurised camel milk and camel urine was significantly and negatively associated with T-cell positivity (odds ratio 0·07, 95% CI 0·01-0·54).

Interpretation: Zoonotic infection of dromedary-exposed individuals is taking place in Nigeria and suggests that the extent of MERS-CoV infections in Africa is underestimated. MERS-CoV could therefore adapt to human transmission in Africa rather than the Arabian Peninsula, where attention is currently focused.

Funding: The National Science and Technology Major Project, National Institutes of Health.
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http://dx.doi.org/10.1016/S1473-3099(20)30599-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7538089PMC
March 2021

Effect of acupuncture versus artificial tears for dry eye disease: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2020 Jul;99(30):e21301

School of Acupuncture-moxibustion and Tuina, Chengdu University of Traditional Chinese Medicine.

Background: The global prevalence of dry eye disease (DED) ranged from 5% to 50%, accompanied by the yearly increasing trend and younger onset. To date artificial tear serves as a mainstay therapy for DED management. It is noteworthy that, acupuncture has been accepted for treating DED with a time-honored history in China. However, no systematic review has been updated till now, which is focusing on comparing acupuncture vs. artificial tears for DED management.

Methods: Eight databases will be searched with the language restrictions of English and Chinese from their inception to July 1, 2020. Randomized controlled trials comparing acupuncture versus artificial tears for DED treatment were enrolled. Identification, research inclusion, data extraction and assessment of the risk of bias were conducted independently by 2 or more reviewers. The primary outcomes were Schirmer I test and tear break-up time. We used Review Manager Software (v.5.3) for assessing the risk of bias and all statistical analyses.

Results: Based on the evidence obtained, whether the effect of acupuncture was equal to or even better than that of artificial tear therapy in the treatment of DED would be elaborated.

Conclusion: In summary, this review would provide a relatively convincing conclusion on whether acupuncture deserves to be recommended as an adjunct treatment for DED, so as to propose some significant insights for the doctors handling with DED.

Osf Registration Number: 10.17605/OSF.IO/Z28M6.
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http://dx.doi.org/10.1097/MD.0000000000021301DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7387016PMC
July 2020

Microwave Hydrothermally Synthesized Metal-Organic Framework-5 Derived C-doped ZnO with Enhanced Photocatalytic Degradation of Rhodamine B.

Langmuir 2020 Aug 16;36(33):9658-9667. Epub 2020 Aug 16.

Integrated Composites Laboratory (ICL), Department of Chemical & Biomolecular Engineering, University of Tennessee, Knoxville, Tennessee 37996, United States.

C-doped ZnO particles have been successfully prepared by the calcination using microwave hydrothermally prepared metal-organic framework-5 (MOF-5) as the precursor. MOF-5 was turned into C-doped ZnO through calcination at 500 °C, and its cubic shape was well-maintained. X-ray photoelectron spectroscopic studies confirmed the C-doping in the ZnO. The as-prepared C-doped ZnO demonstrated a Rhodamine B (RhB) degradation efficiency of 98% in 2 h under an solar-simulated light irradiation, much higher than that of C-doped ZnO derived from MOF-5 synthesized by the ordinary hydrothermal method. The trapping experiment revealed that the crucial factors in the RhB removal were photogenerated h and •O.
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http://dx.doi.org/10.1021/acs.langmuir.0c00395DOI Listing
August 2020

Kinetics of viral load and antibody response in relation to COVID-19 severity.

J Clin Invest 2020 10;130(10):5235-5244

State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital, and.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent for coronavirus 2019 (COVID-19) pneumonia. Little is known about the kinetics, tissue distribution, cross-reactivity, and neutralization antibody response in patients with COVID-19. Two groups of patients with RT-PCR-confirmed COVID-19 were enrolled in this study: 12 severely ill patients in intensive care units who needed mechanical ventilation and 11 mildly ill patients in isolation wards. Serial clinical samples were collected for laboratory detection. Results showed that most of the severely ill patients had viral shedding in a variety of tissues for 20-40 days after onset of disease (8/12, 66.7%), while the majority of mildly ill patients had viral shedding restricted to the respiratory tract and had no detectable virus RNA 10 days after onset (9/11, 81.8%). Mildly ill patients showed significantly lower IgM response compared with that of the severe group. IgG responses were detected in most patients in both the severe and mild groups at 9 days after onset, and remained at a high level throughout the study. Antibodies cross-reactive to SARS-CoV and SARS-CoV-2 were detected in patients with COVID-19 but not in patients with MERS. High levels of neutralizing antibodies were induced after about 10 days after onset in both severely and mildly ill patients which were higher in the severe group. SARS-CoV-2 pseudotype neutralization test and focus reduction neutralization test with authentic virus showed consistent results. Sera from patients with COVID-19 inhibited SARS-CoV-2 entry. Sera from convalescent patients with SARS or Middle East respiratory syndrome (MERS) did not. Anti-SARS-CoV-2 S and N IgG levels exhibited a moderate correlation with neutralization titers in patients' plasma. This study improves our understanding of immune response in humans after SARS-CoV-2 infection.
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http://dx.doi.org/10.1172/JCI138759DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7524490PMC
October 2020

miR-136 improves renal fibrosis in diabetic rats by targeting down-regulation of tyrosine kinase SYK and inhibition of TGF-β1/Smad3 signaling pathway.

Ren Fail 2020 Nov;42(1):513-522

Department of Kidney Transplantation, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

To investigate the way that miR-136 regulated spleen tyrosine kinase (SYK) and transforming growth factor-β1 (TGF-β1)/Smad3 signaling pathways on renal fibrosis. 100 male SD (Sprague-Dawley) rats were randomly divided into diabetic nephropathy (DN) group, normal control (NC) group, miR-136 mimics group, and control group. The renal fibrosis model of diabetic rats was established by streptozotocin (STZ) method. NRK-52E cells were transfected into six groups: HG group, HG + miR-136 group, HG + miR-NC group, miR-136 + SYK group, miR-136 + NC group, and control group. Histopathological examination, the expressions of miR-136 and SYK mRNA, the expression of mTOR, blood glucose, urine protein, body weight, creatinine level, blood urea nitrogen (BUN), and KW/BW were detected in each group. Transfection efficiency, the targeted binding, and regulation between miR-136 and SYK, as well as the expression level of related inflammatory factors, the expression levels of SYK, E-Cad (E-cadherin), Vimentin, Collagen I, α-smooth muscle actin (α-SMA), and vascular endothelial growth factor A (VEGFA) were detected. It was shown that the expression level of miR-136 in DN group significantly decreased. The blood glucose and urine protein concentrations in the DN group and miR-136 mimics group significantly increased and the body weight was decreased, but the blood glucose concentration in the miR-136 mimics group increased with time. The prolongation of the decline significantly decreased, and the growth rate of urinary protein reduced. Creatinine, BUN, and the kidney weight to body weight ratio (KW/BW) in DN group increased significantly. Cell culture results showed that SYK was a target gene of miR-136 and miR-136/SYK-mediated renal fibrosis by activating TGF-β1/Smad3 signal. SYK activates TGF-β1/Smad3 signaling, while miR-136 inhibits TGF-β1/Smad3 signaling mediating tubular epithelial cell fibrosis by down-regulating SYK.
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http://dx.doi.org/10.1080/0886022X.2020.1764854DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946058PMC
November 2020

Comparison of pediatric choledochal cyst excisions with open procedures, laparoscopic procedures and robot-assisted procedures: a retrospective study.

Surg Endosc 2020 07 28;34(7):3223-3231. Epub 2020 Apr 28.

Department of Pediatric Surgery, West China hospital, Sichuan University, Chengdu, China.

Background: The main treatment of choledochal cysts is the complete resection of the cyst with Roux-en-Y hepaticojejunostomy, which includes open procedures, laparoscopic procedures, and robot-assisted procedures using a da Vinci surgical system. The aim of this current study was to investigate the safety and effectiveness of these three different surgical methods in pediatric choledochal cyst excisions.

Methods: Between January 2015 and December 2018, patients with choledochal cysts treated with open procedures, laparoscopic procedures, or robot-assisted procedures were retrospectively analyzed. The data collected included demographic information of all patients, type and size of cyst, operative details, and postoperative outcomes.

Results: A total of 371 episodes of patients were enrolled which consist of the open procedures group (n = 226), laparoscopic procedures group (n = 104), and robot-assisted procedures group (n = 41). The operation time was significantly longer in the laparoscopic procedures group (212.79 ± 34.94) than open procedures group (115.88 ± 13.50) and robot-assisted procedures group (180.61 ± 14.07) (p < 0.001). The volume of intraoperative bleeding were higher in the open procedures group (40.12 ± 55.51) than in the laparoscopic procedures group (21.73 ± 11.44) and robot-assisted procedures group (21.34 ± 9.42), while there was no significant difference between the latter groups. The time to taking water, time to starting liquid diet, and the average length of postoperative hospital stay were similar between the laparoscopic and robot-assisted procedures group, which are shorter than the open procedures group with significant differences. There was no signifcant difference in complications among the three groups.

Conclusion: Choledochal cyst excision with robotic-assisted procedures had identical surgical effects as open procedures and had lower technical requirements. But it had higher medical cost and better cosmetic effects. Open procedures had largely positive surgical outcomes with fewest complications but poor cosmetic effects. Laparoscopic procedures were the most technique-demanding approaches with positive cosmetic and economic effect. The incidence of complications of laparoscopic procedures decreased with the learning curve.
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http://dx.doi.org/10.1007/s00464-020-07560-1DOI Listing
July 2020

Whole genome sequence analysis of the mosquitocidal Bacillus thuringiensis LLP29.

Arch Microbiol 2020 Sep 15;202(7):1693-1700. Epub 2020 Apr 15.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops and, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, People's Republic of China.

Bacillus thuringiensis (Bt) is efficient, strongly specific, and avirulent to humans, making it one of the most popular biopesticides in the world. Bt LLP29 is a mosquitocidal strain that was first isolated from Magnolia denudata. To understand its molecular mechanism against mosquitoes, the genome of Bt LLP29 was sequenced and annotated in this study. The LLP29 genome was found to have a total length of 5.99 Mb, with an average G + C content of 35.21%. A total of 6107 coding sequences were also detected, together with 42 rRNAs and 124 tRNAs and 135 other RNAs. With the help of annotation databases, including GO, COG, KEGG, Nr and Swiss-Prot, most unigene functions were identified. At the same time, a collinear analysis was performed on the genome of LLP29. There were also some virulence genes detected, including cry, chitinase, zwittermicin and vip.
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http://dx.doi.org/10.1007/s00203-020-01875-2DOI Listing
September 2020

A Report of 21 Cases of Laparoscopic Liver Resection in Children.

J Laparoendosc Adv Surg Tech A 2020 May 26;30(5):581-585. Epub 2020 Mar 26.

Department of Pediatric Surgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China.

To summarize and analyze the technique and experience of laparoscopic liver resection (LLR) in children. From Dec 2015 to Dec 2018, surgical outcomes were evaluated in 30 pediatric patients with liver tumors. LLR was performed in 21 patients, including 15 males and 6 females with a mean age of 7.6 ± 3.8 years (range, 2-13 years) and a mean liver tumor diameter of 7.2 ± 2.0 cm (range, 4-10 cm). During the same period, 9 cases of traditional open liver resection were selected as controls, including 6 males and 3 females with a mean age of 7.0 ± 3.7 years (range, 2-13 years) and a mean liver tumor diameter of 7.5 ± 2.2 cm (range, 4.4-10.0 cm). The mean operation time and mean hepatic inflow occlusion time were longer in the laparoscopic group, with values of 182.3 ± 66.1 minutes and 83.2 ± 38.2 minutes, respectively, than in the open group, with values of 120.0 ± 54.3 minutes and 49.6 ± 26.5 minutes, respectively. There were no significant differences between the two groups in blood loss (164.4 ± 107.4 mL versus 133.8 ± 87.1 mL), blood transfusion volume (102.2 ± 100.2 mL versus 69.2 ± 81.8 mL), and alanine aminotransferase levels 24 hours after surgery (212.0 ± 101.4 IU/L versus 173.3 ± 97.2 IU/L) and 72 hours after surgery (74.0 ± 32.5 IU/L versus 81.7 ± 57.3 IU/L). The average hospital stay in the laparoscopic group (6.2 ± 1.6 days) was significantly shorter than that in the open group (8.2 ± 2.0 days). The children in both groups recovered well after surgery without serious complications. LLR contributes to improved recovery of patients after surgery without increasing the incidence of complication, therefore, LLR is a safe option for liver tumors in children.
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http://dx.doi.org/10.1089/lap.2019.0376DOI Listing
May 2020

Stable Interface between a NaCl-AlCl Melt and a Liquid Ga Negative Electrode for a Long-Life Stationary Al-Ion Energy Storage Battery.

ACS Appl Mater Interfaces 2020 Apr 19;12(13):15063-15070. Epub 2020 Mar 19.

State Key Laboratory of Advanced Metallurgy, University of Science and Technology Beijing, Beijing 100083, P. R. China.

Intermediate temperature NaCl-AlCl-based Al-ion batteries are considered as a promising stationary energy storage system due to their low cost, high safety, etc. However, such a cheap electrolyte has a critical feature, i.e., strong corrosion, which results in the short cycle life of the conventional Al-metal anode and also limits the development of the NaCl-AlCl-based Al-ion batteries. A noncorrosive electrolyte may be a good choice for addressing the above challenge, while it is difficult to obtain the electrolyte that has advantages of both noncorrosion and low cost. Therefore, here, we report a Ga-metal anode in the affordable NaCl-AlCl electrolyte for constructing a long-life stationary Al-ion energy storage system. This featured liquid metal anode shows good alloying and dealloying processes between metallic Ga and Al, as well as renders superior stability of the interface between the electrolyte and the anode (e.g., smoothly running for over 580 h at 2 mA cm). No-corrosion and no-pulverization problems appear in this novel liquid/liquid interface. Those advantages demonstrate that the liquid Ga-metal anode has a great promise for the improvement of the NaCl-AlCl-based Al-ion batteries for large-scale stationary energy storage applications.
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http://dx.doi.org/10.1021/acsami.9b21809DOI Listing
April 2020

MicroRNA-489 suppresses osteosarcoma invasion, migration and epithelial-to-mesenchymal transition by directly targeting NAA10.

Minerva Endocrinol 2020 06 13;45(2):150-153. Epub 2019 Nov 13.

Department of Orthopedics, The People's Hospital of Rizhao, Rizhao, China.

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http://dx.doi.org/10.23736/S0391-1977.19.03075-XDOI Listing
June 2020

Determination and detoxification of cyanide in gold mine tailings: A review.

Waste Manag Res 2019 Nov 11;37(11):1117-1126. Epub 2019 Oct 11.

College of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao, China.

Cyanide is among the most toxic chemicals widely employed in the cyanidation process to leach precious minerals, such as gold and silver, by the minerals processing companies worldwide. This present article reviews the determination and detoxification of cyanide found in gold mine tailings. Most of the cyanide remains in the solution or the slurries after the cyanidation process. The cyanide species in the gold tailings are classified as free cyanide, weak acid dissociation, and metallocyanide complexes. Several methods, such as colorimetric, titrimetric, and electrochemical, have been developed to determine cyanide concentrations in gold mine effluents. Application of physical, natural, biological, and chemical methods to detoxify cyanide to a permissible limit (50 mg L) can be achieved when the chemical compositions of cyanide (type of species) present in the tailings are known. The levels of cyanide concentration determine the impact it will have on the environment.
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http://dx.doi.org/10.1177/0734242X19876691DOI Listing
November 2019

Risk factors and associated outcomes of early acute kidney injury in pediatric liver transplant recipients: A retrospective study.

J Pediatr Surg 2020 Mar 13;55(3):446-450. Epub 2019 Aug 13.

West China of Hospital, Sichuan University, Department of Pediatric Surgery, No. 37 Guo Xue Xiang, Chengdu, Sichuan, China. Electronic address:

Background: Acute kidney injury (AKI) may contribute to high mortality rates after liver transplantation. Few studies have investigated AKI in pediatric liver transplantation. This retrospective study was conducted to investigate the risk factors for and associated outcomes of AKI in pediatric liver transplant recipients.

Methods: Eighty pediatric liver transplant patients were included. The occurrence of AKI was defined by the KDIGO Clinical Practice Guidelines for Acute Kidney Injury. A multivariate regression analysis model was used to investigate risk factors for AKI in the pediatric liver recipients.

Results: The final multivariable regression model showed that biliary atresia (odds ratio [OR] = 0.097, p = 0.03), increased time of the anhepatic phase (OR = 0.871, p = 0.005) and lower postoperative jaundice clearance (OR = 13.936, p = 0.02) were independently associated with the development of AKI in pediatric patients. Additionally, cumulative 3-year patient (p = 0.15) and graft (p = 0.26) survival rates between the non-acute kidney injury (NAKI) and AKI groups were 95.2% vs 86.8% and 90.5% vs 84.2%, respectively.

Conclusion: Pediatric liver transplant recipients with a presence of biliary atresia, increased time of anhepatic phase, and a lower postoperative jaundice clearance had an increased risk of AKI. The long-term outcomes of patients who developed AKI appears to be worse compared with those having NAKI.

Type Of Study: Prognosis study.

Level Of Evidence: Level III.
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http://dx.doi.org/10.1016/j.jpedsurg.2019.07.019DOI Listing
March 2020

CTLGA9 Interacts with ALP1 and APN Receptors To Modulate Cry11Aa Toxicity in .

J Agric Food Chem 2019 Aug 6;67(32):8896-8904. Epub 2019 Aug 6.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Key Lab of Biopesticides and Chemical Biology, MOE , Fujian Agriculture and Forestry University , 350002 Fuzhou , Fujian , PR China.

The mosquito is associated with the spread of many viral diseases in humans, including Dengue virus (DENVs), Yellow fever virus (YFV), Zika virus (ZIKV), and Chikungunya virus (CHIKV). (Bt) is widely used as a biopesticide, which produces Cry toxins for mosquito control. The Cry toxins bind mainly to important receptors, including alkaline phosphatase (ALP) and aminopeptidase-N (APN). This work investigated the function of a C-type lectin, CTLGA9, in in response to Cry toxins. Our results showed by far-western blot and ELISA methods that the CTLTGA9 protein interacted with brush border membrane vesicles (BBMVs) of larvae and with ALP1, APN, and Cry11Aa proteins. Furthermore, molecular docking showed overlapping binding sites in ALP1 and APN for binding to Cry11Aa and CTLGA9. The toxicity assays further demonstrated that CTLGA9 inhibited the larvicidal activity of Cry toxins. According to the results of molecular docking, CTLGA9 may compete with Cry11Aa for binding to ALP1 and APN receptors and thus decreases the mosquitocidal toxicity of Cry11Aa. Our results provide further insights into better understanding the mechanism of Cry toxins and help improve the Cry toxicity for mosquito control.
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http://dx.doi.org/10.1021/acs.jafc.9b01840DOI Listing
August 2019

High-efficiency transformation of amorphous carbon into graphite nanoflakes for stable aluminum-ion battery cathodes.

Nanoscale 2019 Jul 6;11(26):12537-12546. Epub 2019 Jun 6.

State Key Laboratory of Advanced Metallurgy, University of Science and Technology Beijing, Beijing, 100083, PR China.

Highly efficient strategies for the transformation of amorphous carbon into graphite with high graphitization and crystallinity features have been significantly pursued in recent years; however, critical issues, including high processing temperature, insufficient graphitization, introduction of catalyst impurities, complicated post-purification procedures, and generation of greenhouse gas, still remain in traditional approaches. For significantly addressing these challenges, herein, a highly efficient catalyst-free, eco-friendly and low-temperature electrochemical transformation strategy was proposed for the preparation of highly graphitized porous graphite nanoflakes. Using inert SnO as an anode in CaCl-LiCl molten salts, the graphitization transformation of amorphous carbon materials could be realized at 700 °C, approaching the record in high-efficiency converting amorphous carbon to graphite; moreover, systematical analysis was performed to understand the electrochemical transformation of amorphous carbon into highly graphitized graphite nanoflakes. For extending their valuable applications, the as-prepared graphite nanoflakes were further utilized as cathodes in aluminum-ion batteries, which exhibited significantly promising energy storage performance; moreover, an initial discharge capacity of 63.6 mA h g at a current density of 200 mA g was achieved, which eventually became 55.5 mA h g with a coulombic efficiency of 95.4% after 1000 cycles; thus, these cathodes exhibited stable long-term cycling performance. The combination of low-temperature electrochemical transformation and the subsequent high-performance applications of these nanoflakes in energy storage indicates that the proposed strategy is highly efficient for the transformation and utilization of abundant amorphous carbon resources for the realization of high value-added applications.
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http://dx.doi.org/10.1039/c9nr03112jDOI Listing
July 2019

EMP1 regulates cell proliferation, migration, and stemness in gliomas through PI3K-AKT signaling and CD44.

J Cell Biochem 2019 10 20;120(10):17142-17150. Epub 2019 May 20.

Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.

Glioblastoma multiforme (GBM) is an intracranial tumor; the feature is higher malignant and poorer prognosis. The search for therapeutic targets for gliomas has always been a focus of research in the field of neurology. The unusual expression of epithelial membrane protein 1 (EMP1) has been proved in most tumors. In our study, we determined the expression level of EMP1 expression in glioma tissues. There were higher levels of EMP1 in glioma tissues-particularly GBM tissues-than those in normal brain tissues. Then we discovered that silencing EMP1 inhibited glioma cell invasion and proliferation through inhibiting the PI3K-AKT signaling pathway. Subsequently, we investigated the function of EMP1 on glioma stem cells and found that it regulates the expression of CD44 in such cells to promote stemness. Taken together, the new strategies for the treatment of glioma may be provided by these finding, thereby improving the prognosis associated with it.
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http://dx.doi.org/10.1002/jcb.28974DOI Listing
October 2019

Post-traumatic stress disorder in living donors after pediatric liver transplantation: A cross-sectional investigation study.

Medicine (Baltimore) 2019 May;98(20):e15565

Department of Vascular Surgery.

Liver transplantation can lead to post-traumatic stress disorder (PTSD) in recipients, but the risk factors associated with PTSD in living donors are unknown. To investigate this progression in pediatric living donors, a cross-sectional investigation was carried out.All participants completed 2 questionnaires: a PTSD self-rating scale (PTSD-SS) and a validated Chinese version of the Medical Outcomes Study Short Form-36 (SF-36). Clinical and demographic data were collected from medical records and self-report questionnaires. Univariate analysis was conducted to identify statistical differences.The prevalence of full PTSD (all symptom clusters) and partial PTSD (2 out of 3 symptom clusters) was 12.1% and 31.1%, respectively. Those with an educational status of elementary school (P = .001), who were donors to their children (P = .008), who were in the first 6 months after transplant (P < .001), or were involved in transplants where the recipients had severe complications (P = .02) were more likely to have higher PTSD-SS scores than other groups. The non-PTSD group had a higher health-related quality-of-life score compared with the full and partial PTSD groups in the domains of physical function, role-physical, bodily pain, general health, vitality, social functioning, role-emotional, and mental health. In addition, the occurrence of PTSD was related to a poorer quality of life.The occurrence of PTSD was common in living donors after pediatric liver transplantation. Those with a lower educational status, who were donors to their children, were in the first 6 months after transplant, or were involved in transplants where the recipients had severe complications were most likely to experience PTSD. Post-traumatic stress symptom severity was significantly associated with a poorer quality of life after transplant.
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http://dx.doi.org/10.1097/MD.0000000000015565DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6531068PMC
May 2019

Inhibition of lncRNA MEG3 protects renal tubular from hypoxia-induced kidney injury in acute renal allografts by regulating miR-181b/TNF-α signaling pathway.

J Cell Biochem 2019 08 12;120(8):12822-12831. Epub 2019 Mar 12.

Department of Kidney Transplantation, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Early damage to transplanted organs initiates excess inflammation that deteriorates existing injury, which is a leading cause of graft loss. Long noncoding RNAs (lncRNAs) are recently thought to play a significant role in cellular homeostasis during pathological process of kidney diseases. The aim of this study was to assess the function and mechanism of lncRNA, maternally expressed gene 3 (MEG3), on early renal allografts pathogenesis. Real-time polymerase chain reaction (RT-PCR) analysis found that the levels of MEG3 and miR-181b-5p were increased and decreased respectively in grafted kidney. The Western blot assay showed that TNF-alpha was upregulated in the kidney and in HK-2 cells. Administering MEG3-specific small interfering RNA (siRNA) in mice silenced MEG3 expression and protected kidney renal allograft from injury. Bioinformatical analysis and luciferase assay indicated that MEG3 is a target of miR-181b-5p. MEG3 inhibition and overexpression promoted and suppressed miR-181b-5p levels respectively. In addition, Western blot and immunohistochemical staining suggested that decreased TNF-alpha expression was observed in the kidney. In contrary to MEG3, miR181b overexpression attenuated hypoxia-induced HK-2 cell apoptosis, as well as suppressed hypoxia-induced TNF-alpha upregulation. In luciferase reporter assay, we confirmed that miR-181b directly bound to the 3'-untranslated region (3'-UTR) of TNF-alpha, thereby negatively regulating the TNF-alpha expression. Our data suggested that MEG3 functions as a competing endogenous RNA for miR-181b to regulate the TNF-alpha expression in hypoxia-induced kidney injury in acute renal allografts.
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http://dx.doi.org/10.1002/jcb.28553DOI Listing
August 2019

A Low-Power Dynamic-Range Relaxed Analog Front End for Photoplethysmogram Acquisition.

Annu Int Conf IEEE Eng Med Biol Soc 2018 Jul;2018:5554-5557

This paper presents a low-power analog front-end that enables photoplethysmographic signals acquisition, the dynamic range for AC component exaction is relaxed with simple high-pass implementation. The chopping modulation ensures the low-noise operation. The circuit is fabricated in a 0.18-um CMOS technology. Measurements show that the consuming current is approximately 72 uA at a supply of 2.5 V. The circuit achieves a input noise of 6.45 $\mathrm{pA}/\sqrt{Hz}$. The calibred algorithm is implemented by means of MCU, and the demonstration that is compared with the Fluck Simulator used as the reference shows the heart rate is accurately detected, and the error of the measured blood oxygen saturation is less than 1.5%.
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http://dx.doi.org/10.1109/EMBC.2018.8513613DOI Listing
July 2018

Disability and health-related quality of life in Chinese patients with rheumatoid arthritis: A cross-sectional study.

Int J Rheum Dis 2018 Sep;21(9):1709-1715

Department of Rheumatology and Immunology, Peking University People's Hospital, Beijing, China.

Aim: The objective of this study was to evaluate the impact of rheumatoid arthritis (RA) on physical function and health-related quality of life (HRQoL) in China.

Method: A cross-section survey was conducted in 21 general hospitals in China. Eight hundred and seven patients were recruited. Data on demographics, clinical data, physical function (Stanford Health Assessment Questionnaire Disability Index, HAQ-DI) and HRQoL (Study Short Form 36 Health Survey, SF-36) were collected on site.

Results: In our cohort, physical function was impaired in 77.6% of patients (HAQ-DI >0). The median (interquartile range, IQR) of HAQ-DI was 0.750 (0.125, 1.500). Rated by HAQ-DI 0-1, >1-2, and >2-3, percentage of patients with mild, moderate and severe disability was 61.0%, 25.4%, and 13.6%, respectively. Older age, long disease duration, presence of extra-articular manifestations, tender joint count (TJC), overall status (assessed by patient Global Visual Analogue Scale [G-VAS] and physician G-VAS) and lacking disease-modifying anti-rheumatic drugs were identified as predictive factors for worse physical function (P < .05). The composite scores of SF-36 in the observed patients were: physical component summary 40.4 (IQR 27.4, 60.3), and mental component summary 49.0 (IQR 33.6, 70.9). Impaired physical health may be predicted by low income, presence of extra-articular manifestations, TJC, patient G-VAS and high HAQ-DI. Predictors for suboptimal mental health were low income, physical labor, married status, increased swollen joint count (SJC), physician G-VAS and high HAQ-DI.

Conclusion: Rheumatoid arthritis has profound effects on physical function and HRQoL in Chinese patients. Patients with identified predictive factors for poor outcome should be closely monitored.
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http://dx.doi.org/10.1111/1756-185X.13345DOI Listing
September 2018

C-Type Lectin-20 Interacts with ALP1 Receptor to Reduce Cry Toxicity in .

Toxins (Basel) 2018 09 25;10(10). Epub 2018 Sep 25.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Key Lab of Biopesticides and Chemical Biology, MOE, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China.

is a crucial vector for human diseases, such as yellow fever, dengue, chikungunya, and Zika viruses. Today, a major challenge throughout the globe is the insufficient availability of antiviral drugs and vaccines against arboviruses, and toxins produced by (Bt) are still used as biological agents for mosquito control. The use of Cry toxins to kill insects mainly depends on the interaction between Cry toxins and important toxin receptors, such as alkaline phosphatase (ALP). In this study, we investigated the function of C-type lectin-20 (CTL-20) in the tolerance of Cry toxins. We showed that recombinant CTL-20 protein interacted with both Cry11Aa and ALP1 by the Far-Western blot and ELISA methods, and CTL-20 bound to larval brush border membrane vesicles (BBMVs). Binding affinity of CTL-20 to ALP1 was higher than that of Cry11Aa to ALP1. Furthermore, the survival rate of larvae fed with Cry11Aa toxin mixed with recombinant CTL-20 fusion protein was significantly increased compared with that of the control larvae fed with Cry11Aa mixed with thioredoxin. Our novel results suggest that midgut proteins like CTLs may interfere with interactions between Cry toxins and toxin receptors by binding to both Cry toxins and receptors to alter Cry toxicity.
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http://dx.doi.org/10.3390/toxins10100390DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6215184PMC
September 2018

Transcriptomic Analysis of Aedes aegypti in Response to Mosquitocidal Bacillus thuringiensis LLP29 Toxin.

Sci Rep 2018 08 23;8(1):12650. Epub 2018 Aug 23.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Key Lab of Biopesticides and Chemical Biology, MOE, Fujian Agriculture and Forestry University, 350002, Fuzhou, Fujian, PR China.

Globally, Aedes aegypti is one of the most dangerous mosquitoes that plays a crucial role as a vector for human diseases, such as yellow fever, dengue, and chikungunya. To identify (1) transcriptomic basis of midgut (2) key genes that are involved in the toxicity process by a comparative transcriptomic analysis between the control and Bacillus thuringiensis (Bt) toxin (LLP29 proteins)-treated groups. Next-generation sequencing technology was used to sequence the midgut transcriptome of A. aegypti. A total of 17130 unigenes, including 574 new unigenes, were identified containing 16358 (95.49%) unigenes that were functionally annotated. According to differentially expressed gene (DEG) analysis, 557 DEGs were annotated, including 226 upregulated and 231 downregulated unigenes in the Bt toxin-treated group. A total of 442 DEGs were functionally annotated; among these, 33 were specific to multidrug resistance, 6 were immune-system-related (Lectin, Defensin, Lysozyme), 28 were related to putative proteases, 7 were lipase-related, 8 were related to phosphatases, and 30 were related to other transporters. In addition, the relative expression of 28 DEGs was further confirmed through quantitative real time polymerase chain reaction. The results provide a transcriptomic basis for the identification and functional authentication of DEGs in A. aegypti.
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http://dx.doi.org/10.1038/s41598-018-30741-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107635PMC
August 2018

Adverse event detection by integrating twitter data and VAERS.

J Biomed Semantics 2018 06 20;9(1):19. Epub 2018 Jun 20.

Department of Epidemiology & Public Health, University of Maryland School of Medicine, Baltimore, MD, USA.

Background: Vaccine has been one of the most successful public health interventions to date. However, vaccines are pharmaceutical products that carry risks so that many adverse events (AEs) are reported after receiving vaccines. Traditional adverse event reporting systems suffer from several crucial challenges including poor timeliness. This motivates increasing social media-based detection systems, which demonstrate successful capability to capture timely and prevalent disease information. Despite these advantages, social media-based AE detection suffers from serious challenges such as labor-intensive labeling and class imbalance of the training data.

Results: To tackle both challenges from traditional reporting systems and social media, we exploit their complementary strength and develop a combinatorial classification approach by integrating Twitter data and the Vaccine Adverse Event Reporting System (VAERS) information aiming to identify potential AEs after influenza vaccine. Specifically, we combine formal reports which have accurately predefined labels with social media data to reduce the cost of manual labeling; in order to combat the class imbalance problem, a max-rule based multi-instance learning method is proposed to bias positive users. Various experiments were conducted to validate our model compared with other baselines. We observed that (1) multi-instance learning methods outperformed baselines when only Twitter data were used; (2) formal reports helped improve the performance metrics of our multi-instance learning methods consistently while affecting the performance of other baselines negatively; (3) the effect of formal reports was more obvious when the training size was smaller. Case studies show that our model labeled users and tweets accurately.

Conclusions: We have developed a framework to detect vaccine AEs by combining formal reports with social media data. We demonstrate the power of formal reports on the performance improvement of AE detection when the amount of social media data was small. Various experiments and case studies show the effectiveness of our model.
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http://dx.doi.org/10.1186/s13326-018-0184-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6011255PMC
June 2018

Comparison of the impact of Tripterygium wilfordii Hook F and Methotrexate treatment on radiological progression in active rheumatoid arthritis: 2-year follow up of a randomized, non-blinded, controlled study.

Arthritis Res Ther 2018 04 10;20(1):70. Epub 2018 Apr 10.

Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Clinical Immunology Center, Chinese Academy of Medical Sciences and Peking Union Medical College, The Ministry of Education Key Laboratory, Beijing, 100730, China.

Background: Tripterygium wilfordii Hook F (TwHF) alone or in combination with methotrexate (MTX) has been shown to be more effective than MTX monotherapy in controlling the manifestations in subjects with disease-modifying antirheumatic drug (DMARD)-naïve active rheumatoid arthritis (RA) over a 6-month period. The long-term impact of these therapies on disease activity and radiographic progression in RA has not been examined.

Methods: Patients with DMARD-naïve RA enrolled in the "Comparison of Tripterygium wilfordii Hook F with methotrexate in the Treatment of Active Rheumatoid Arthritis" (TRIFRA) study were randomly allocated into three arms with TwHF or MTX or the two in combination. Clinical indexes and radiographic data at baseline and year 2 was collected and compared using an intent-to-treat (ITT) and a per-protocol (PP) analysis. Two radiologists blinded to the treatment scored the images independently.

Results: Of 207 subjects 109 completed the 2-year follow up. The number of subjects withdrawing from the study and the number adhering to the initial regimens were similar among the three groups (p > = 0.05). In the ITT analysis, proportions of patients reaching American College of Rheumatology 50% (ACR50) response criteria were 46.4%, 58.0% and 50.7% in the MTX, TwHF and MTX + TwHF groups (TwHF vs MTX monotherapy, p = 0.004). Similar patterns were found in ACR20, ACR70, Clinical Disease Activity Index good responses, European League Against Rheumatism good response, remission rate and low disease activity rate at year 2. The results of the PP analysis agreed with those in the ITT analysis. The changes in total Sharp scores and joint erosion and joint space narrowing during the 2 years were associated with changes in disease activity measured by the 28-joint count Disease Activity Score and were comparable among the three groups (p > 0.05). Adverse events were similar in the three treatment groups.

Conclusions: During the 2-year therapy period, TwHF monotherapy was not inferior to MTX monotherapy in controlling disease activity and retarding radiological progression in patients with active RA.

Trial Registration: This is a follow-up study. Original trial registration: ClinicalTrials.gov , NCT01613079 . Registered on 4 June 2012.
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http://dx.doi.org/10.1186/s13075-018-1563-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5894170PMC
April 2018