Publications by authors named "Junwei Liu"

127 Publications

Effect of Pegylated Interferon Plus Tenofovir Combination on Higher Hepatitis B Surface Antigen Loss in Treatment-naive Patients with Hepatitis B e Antigen -positive Chronic Hepatitis B: A Real-world Experience.

Clin Ther 2021 Jan 27. Epub 2021 Jan 27.

Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, China. Electronic address:

Purpose: The loss of serum hepatitis B surface antigen (HBsAg) in patients with chronic hepatitis B (CHB) is considered an ideal clinical outcome but rarely achieved with current standard of care. We evaluated the effectiveness in inducing HBsAg seroclearance in a real-world clinical cohort of Chinese patients with CHB treated with a combination of pegylated interferon (Peg-IFN) with tenofovir disoproxil fumarate (TDF) or monotherapy with each agent.

Methods: A total of 330 patients with CHB were assigned to receive Peg-IFN plus TDF for 48 weeks (Peg-IFN plus TDF group), Peg-IFN alone for 48 weeks (Peg-IFN group), or TDF alone for 144 weeks (TDF group). The primary end point was the percentages of patients who achieved HBsAg seroclearance at week 72. Differences from the baseline characteristics and treatment data were compared using the χ test for categorical variables or 1-way ANOVA for continuous variables. A Kaplan-Meier test was performed to compare the HBsAg loss among the 3 groups. Discrimination of responders versus nonresponders was quantified using AUC curves. Optimal cut-offs were selected based on Youden's J statistic defined as J = sensitivity + specificity-1.

Findings: At week 72, the Kaplan-Meier cumulative HBsAg loss was 11.5% in the Peg-IFN plus TDF group, 5.7% in the Peg-IFN group, and 0% in the TDF group. The percentage of patients with HBsAg loss was comparable in the Peg-IFN plus TDF and Peg-IFN groups (P = 0.143), but both were significantly higher than that in the TDF group (P = 0.000 and P = 0.010). In addition, a significantly higher percentage of patients in the combination group and Peg-IFN group had serum HBsAg of <100 IU/mL compared with the TDF group (32.7% vs 23.6% vs 9.2%; P < 0.001) but no significant differences in the percentages of patients with HBsAg <1000 IU/mL, the undetectable serum HBV DNA and hepatitis B e antigen seroconversion. Our model predicted serum HBsAg loss at week 72 (AUC = 0.846) if the HBsAg level was reduced by > 1.5 log IU/mL from baseline at treatment week 24, an optimal timepoint for prediction of HBsAg loss in this cohort.

Implications: A 48-week course of Peg-IFN and TDF combination therapy led to profound reduction in serum HBsAg level, resulting in a significantly higher rate of HBsAg loss compared with TDF monotherapy. Patients with steep HBsAg decline >1.5 log IU/mL at week 24 well signaled a higher probability of achieving HBsAg loss at week 72.
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http://dx.doi.org/10.1016/j.clinthera.2020.12.022DOI Listing
January 2021

SIRT3 as a potential therapeutic target for heart failure.

Pharmacol Res 2021 Jan 27:105432. Epub 2021 Jan 27.

Cardiovascular Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan, Hubei, 430071, People's Republic of China. Electronic address:

Heart failure causes significant morbidity and mortality worldwide. The underlying mechanisms and pathological changes associated with heart failure are exceptionally complex. Despite recent advances in heart failure research, treatment outcomes remain poor. The sirtuin family member sirtuin-3 (SIRT3) is involved in several key biological processes, including ATP production, catabolism, and reactive oxygen species detoxification. In addition to its role in metabolism, SIRT3 regulates cell death and survival and has been implicated in the pathogenesis of cardiovascular diseases. Emerging evidence also shows that SIRT3 can protect cardiomyocytes from hypertrophy, ischemia-reperfusion injury, cardiac fibrosis, and impaired angiogenesis. In this review article, we summarize the recent advances in SIRT3 research and discuss the role of SIRT3 in heart failure. We also discuss the potential use of SIRT3 as a therapeutic target in heart failure.
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http://dx.doi.org/10.1016/j.phrs.2021.105432DOI Listing
January 2021

Role of ferroptosis induced by a high concentration of calcium oxalate in the formation and development of urolithiasis.

Int J Mol Med 2021 Jan 23;47(1):289-301. Epub 2020 Oct 23.

Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

Ferroptosis is an iron‑dependent lipid peroxidation process. Although the involvement of ferroptosis in kidney diseases has recently been reported, the association between ferroptosis and urolithiasis remains unclear. The present study examined the effects of ferroptosis on calcium oxalate (CaOx) crystal‑induced renal tubular epithelial cell injury in vivo and in vitro. First, renal tubular epithelial cells were exposed to various concentrations of CaOx. By measuring cell viability, Fe2+ levels, lipid peroxidation levels and the levels of ferroptosis‑related proteins, it was identified that the relative expression of the ferroptosis agonist proteins, p53, long‑chain acyl‑CoA synthetases (ACSL4), transferrin (TF) and transferrin receptor (TRC), increased, while the relative expression of the ferroptosis inhibitory proteins, solute carrier family 7 member 11 (SLC7A11, XCT) and glutathione peroxidase 4 (GPX4), decreased significantly. Furthermore, the levels of Fe2+ and lipid peroxidation gradually increased, while cell viability significantly decreased. From these results, it was noted that the extent of CaOx‑induced ferroptosis activation and cell injury was dependent on the CaOx concentration. To further investigate the association between ferroptosis and renal tubular epithelial cell injury, the ferroptosis agonist, erastin, and the ferroptosis inhibitor, ferrostatin‑1, were used to regulate the degree of ferroptosis at the same CaOx concentration in in vivo and in vitro experiments. CaOx‑induced ferroptosis and damage to renal tubular epithelial cells and renal tissue were investigated. Finally, it was identified that through the regulation of ferroptosis levels, renal tubular epithelial cell injury increased significantly when the ferroptosis level increased, and vice versa. On the whole, the present results indicated that ferroptosis is essential for renal tubular epithelial cell injury induced by CaOx crystals. This finding is highly significant and promotes the further investigation of the association between ferroptosis and urolithiasis.
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http://dx.doi.org/10.3892/ijmm.2020.4770DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7723503PMC
January 2021

Epitaxial Growth of Single-Phase 1T'-WSe Monolayer with Assistance of Enhanced Interface Interaction.

Adv Mater 2021 Feb 31;33(7):e2004930. Epub 2020 Dec 31.

National Laboratory of Solid State Microstructure, School of Physics, Nanjing University, Nanjing, 210093, China.

The WSe monolayer in 1T' phase is reported to be a large-gap quantum spin Hall insulator, but is thermodynamically metastable and so far the fabricated samples have always been in the mixed phase of 1T' and 2H, which has become a bottleneck for further exploration and potential applications of the nontrivial topological properties. Based on first-principle calculations in this work, it is found that the 1T' phase could be more stable than 2H phase with enhanced interface interactions. Inspired by this discovery, SrTiO (100) is chosen as substrate and WSe monolayer is successfully grown in a 100% single 1T' phase using the molecular beam epitaxial method. Combining in situ scanning tunneling microscopy and angle-resolved photoemission spectroscopy measurements, it is found that the in-plane compressive strain in the interface drives the 1T'-WSe into a semimetallic phase. Besides providing a new material platform for topological states, the results show that the interface interaction is a new approach to control both the structure phase stability and the topological band structures of transition metal dichalcogenides.
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http://dx.doi.org/10.1002/adma.202004930DOI Listing
February 2021

Tandem mass tag-based quantitative proteomic profiling of the serum of patients with abnormal uterine bleeding associated with copper intrauterine device.

Int J Gynaecol Obstet 2020 Dec 22. Epub 2020 Dec 22.

NHC Key Lab of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), School of Pharmacy, Fudan University, Shanghai, China.

Objective: To investigate changes in the level of protein in serum and uncover the underlying pathogenesis of abnormal uterine bleeding (AUB) associated with copper intrauterine devices (Cu IUD).

Methods: Protein profiles were investigated via tandem mass tag (TMT)-based quantitative proteomics and bioinformatics technology. Quantification and characterization of candidate proteins were further performed in 33 controls and 45 cases by Luminex assay and enzyme-linked immunosorbent assay.

Results: In total, 842 proteins were identified via TMT coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the serum of individuals with IUDs. Among them, 25 differentially expressed proteins (p < 0.05) were observed, including eight upregulated proteins and 17 downregulated proteins. Ten proteins were verified, and Alpha-1-Antitrypsin (a1AT) had a significantly elevated expression in women with AUB associated with the Cu IUD compared with healthy controls (p = 0.026) and a high area under the curve value (0.656), as well as sensitivity (64.9%) and specificity (71.9%).

Conclusion: This is the first study to explore changes in serum protein and the underlying mechanisms of AUB associated with the Cu IUD via TMT technology. a1AT with biomarker potential was validated. These findings might provide an experimental basis for the early diagnosis or treatment of AUB associated with the Cu IUD.
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http://dx.doi.org/10.1002/ijgo.13562DOI Listing
December 2020

COVID-19 emergencies around the globe: China's experience in controlling COVID-19 and lessons learned.

Int J Qual Health Care 2021 Feb;33(1)

Vice president, Vice secretary of Communist Party Committee, Zhuhai People's Hospital (Zhuhai Hospital affiliated with Jinan University), No. 79 Kangning Road, Xiangzhou District, Zhuhai, 519000, GP, China.

Motivation: Nations around the world have been significantly impacted during the COVID-19 pandemic. China's strategies for controlling COVID-19 offer valuable lessons for the global community. By learning from China's experience and lessons, other countries could also find appropriate methods to control the pandemic.

Problem Statement: What measures has China taken to control the pandemic? What lessons has China learned through this pandemic?

Approach/methods: The literature on China's lessons and experience in controlling the COVID-19 pandemic was searched and reviewed. Related newspapers and magazines were also searched.

Results: China's experience can be summed up as establishing temporary hospitals, strict isolation, experts with a knowledge of COVID-19, and measures that increase social distancing.

Conclusions: By learning from the experience of China, other countries in the world could eventually find the methods to control the COVID-19 pandemic. An emergency response system should be established in each country. Doctors and nurses are not alone in fighting COVID-19, and the entire world is helping them. With cooperation, current difficulties could be overcome.
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http://dx.doi.org/10.1093/intqhc/mzaa143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7799047PMC
February 2021

Isolated tricuspid valve surgery after congenital versus left heart-disease surgery: mid-term outcomes.

J Thorac Dis 2020 Oct;12(10):5561-5570

Department of Cardiovascular Surgery, Wuhan Union Hospital, Huazhong University of Science and Technology, Wuhan, China.

Background: Left heart involvement might be a differential factor in the physiopathology and prognosis of severe tricuspid regurgitation (TR) following cardiac surgery. We aimed to compare the outcomes of isolated tricuspid valve surgery (ITVS) after congenital versus left heart-disease surgery.

Methods: We retrospectively studied and followed up 58 patients who underwent ITVS for TR following cardiac surgery in our center from January 2012 to December 2017. According to the different etiologies of TR, the participants were divided into one group of TR following surgery for congenital heart diseases (CHD) (pCHD group, n=24), and another group of TR following surgery for left heart disease (pVHD group, n=34).

Results: Compared to the pCHD group, the pVHD group presented with a more advanced age (P<0.001), higher model for end-stage liver disease (MELD) score calculation (P=0.04), and higher EuroSCORE II calculation (P=0.01). In the post-operative course, the pVHD group showed a longer mechanical ventilation time (P<0.001) and longer intensive care unit stay (P=0.001). However, there was no significant difference between the two groups in in-hospital mortality (8.8% 0, P=0.26), or the incidence of major adverse cardiac and cerebrovascular events (MACCE) (20.6% 12.5%, P=0.47) at follow-up.

Conclusions: Severe TR following surgery for left heart disease is associated with higher surgical risks and a remarkable frailty as compared to that following surgery for CHDs; however, with the development of surgical techniques and peri-operative management, ITVS can be safely performed in both conditions with promising contemporary mid-term outcomes.
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http://dx.doi.org/10.21037/jtd-20-1787DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7656346PMC
October 2020

Early outcomes of Stanford type A aortic dissection under the coronavirus disease 2019 (COVID-19) pandemic: a multicentre study from Hubei province.

Interact Cardiovasc Thorac Surg 2020 12;31(6):834-840

Department of Cardiovascular Surgery, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Objectives: Our goal was to compare the short-term outcomes of Stanford type A aortic dissection (TAAD), during the coronavirus disease 2019 (COVID-19) pandemic with those during normal times and summarize our perioperative management experience of patients with TAAD in the context of COVID-19.

Methods: From 17 January 2020 to 8 March 2020, a total of 27 patients with TAAD were operated on in 8 cardiovascular surgery centres in Hubei Province (COVID-19 group). The data from 91 patients with TAAD from the same centres during the same period last year were extracted from the Hubei Cardiac Surgery Registration System (control group). A propensity score matched subgroup of 26 pairs (1:2) was identified. Perioperative data and short-term outcomes were assessed.

Results: Nine patients in the COVID-19 group were categorized as suspicious for the disease (9/27, 33.3%), and others were excluded (18/27, 66.7%). No one was laboratory confirmed preoperatively. The average waiting, cross-clamp and circulatory arrest times were longer in the COVID-19 group (22.9 ± 8.3 vs 9.7 ± 4.0 h, P < 0.001; 135 ± 36 vs 103 ± 45 min, P = 0.003; 24 ± 9 vs 17 ± 8 min, P < 0.001, respectively). The 30-day or in-hospital deaths were 3.8% in both groups (P = 1.0). The COVID-19 group was associated with longer ventilation and intensive care unit times (81 ± 71 vs 45 ± 19 h, P < 0.001; 7.4 ± 3.8 vs 4.5 ± 2.7 days; P < 0.001, respectively). There were no statistical differences between the 2 groups in the incidence of complications such as stroke, neurological deficit, acute kidney injury, pulmonary infection and reoperation. Serum antibody tests for those patients showed 7 out of 9 suspected cases were Immunoglobulin G positive. No cross-infection occurred in other patients or associated medical staff.

Conclusions: With adequate preparation and appropriate protection, satisfactory early outcomes can be achieved after emergency operations for patients with TAAD during the COVID-19 pandemic.
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http://dx.doi.org/10.1093/icvts/ivaa209DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7665539PMC
December 2020

Application of the Hybrid Operating Room in Surgery: A Systematic Review.

Authors:
Hao Jin Junwei Liu

J Invest Surg 2020 Nov 4:1-12. Epub 2020 Nov 4.

Zhuhai Health Bureau, Zhuhai, Guangdong, China.

Background And Objective: The hybrid operating room has been widely applied in surgery, including neurology, general surgery, gynecology, and obstetrics. By reviewing application of the hybrid operating room in different categories of surgery, we aim to summarize both advantages and disadvantages of the hybrid operating room and discuss what to do for further improving the application of it.

Methods: We searched related literature in websites including Pubmed, MEDLINE, Web of science, using the keywords "hybrid operating room", "surgery", "technique", "intervention", and "radiology". All the searched papers were screened and underwent quality evaluation. The eventually selected papers were carefully read, with related information extracted and summarized.

Results: After screening and assessment, a total of 29 literature was collected. Application of the hybrid operating room in general surgery, neurosurgery, thoracic surgery, urology, gynecologic and obstetrics surgery, and cardiovascular surgery was summarized. Both advantages and disadvantages of the hybrid operating room were discussed in order to improve application of the hybrid operating room in surgery.

Conclusions: Surgeries performed in the hybrid operating room take advantages over those conventional operating rooms mainly in terms of higher procedure accuracy, less operative time, and less risk of hemorrhage during the transportation between radiology departments and operating suites. Further efforts should be made to reduce radiation exposure from imaging systems equipped in the hybrid operating room and increase cost-effectiveness ratio of the hybrid operating room.
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http://dx.doi.org/10.1080/08941939.2020.1838004DOI Listing
November 2020

Epigenetic alternations of microRNAs and DNA methylation contribute to gestational diabetes mellitus.

J Cell Mol Med 2020 Dec 21;24(23):13899-13912. Epub 2020 Oct 21.

NHC Key Lab. of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research), Pharmacy School, Fudan University, Shanghai, China.

This study aimed to identify epigenetic alternations of microRNAs and DNA methylation for gestational diabetes mellitus (GDM) diagnosis and treatment using in silico approach. Data of mRNA and miRNA expression microarray (GSE103552 and GSE104297) and DNA methylation data set (GSE106099) were obtained from the GEO database. Differentially expressed genes (DEGs), differentially expressed miRNAs (DEMs) and differentially methylated genes (DMGs) were obtained by limma package. Functional and enrichment analyses were performed with the DAVID database. The protein-protein interaction (PPI) network was constructed by STRING and visualized in Cytoscape. Simultaneously, a connectivity map (CMap) analysis was performed to screen potential therapeutic agents for GDM. In GDM, 184 low miRNA-targeting up-regulated genes and 234 high miRNA-targeting down-regulated genes as well as 364 hypomethylation-high-expressed genes and 541 hypermethylation-low-expressed genes were obtained. They were mainly enriched in terms of axon guidance, purine metabolism, focal adhesion and proteasome, respectively. In addition, 115 genes (67 up-regulated and 48 down-regulated) were regulated by both aberrant alternations of miRNAs and DNA methylation. Ten chemicals were identified as putative therapeutic agents for GDM and four hub genes (IGF1R, ATG7, DICER1 and RANBP2) were found in PPI and may be associated with GDM. Overall, this study identified a series of differentially expressed genes that are associated with epigenetic alternations of miRNA and DNA methylation in GDM. Ten chemicals and four hub genes may be further explored as potential drugs and targets for GDM diagnosis and treatment, respectively.
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http://dx.doi.org/10.1111/jcmm.15984DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7753873PMC
December 2020

Experimental Study on Whole Wind Power Structure with Innovative Open-Ended Pile Foundation under Long-Term Horizontal Loading.

Sensors (Basel) 2020 Sep 18;20(18). Epub 2020 Sep 18.

Shandong Bureau of China Metallurgical Geology Bureau, Jinan 250014, China.

The offshore wind energy (OWE) pile foundation is mainly a large diameter open-ended single pile in shallow water, which has to bear long-term horizontal cyclic loads such as wind and waves during OWE project lifetime. Under the complex cyclic loads, the stress and displacement fields of the pile-soil system change continuously, which affects the dynamic characteristics of the pile foundation. Within the service life of the pile foundation, the pile-soil system has irreversible cumulative deformation, which further causes damage to the whole structure. Therefore, it is important to examine the overall dynamic characteristics of wind power foundation under high cycle. In this paper, in the dry sand foundation, taking the Burbo Bank 3.6 MW offshore turbine-foundation structure as the prototype, the horizontal cyclic loading model tests of the wind power pile foundation with the scale of 1:50 were carried out. Considering the factors such as loading frequency and cyclic load ratio, the horizontal dynamic characteristics of the whole OWE pile foundation are studied. The comparison results between the maximum bending moment of pile and the fitting formula are discussed. In conclusion, moment of OWE pile shaft is corresponding to the loading frequency ( = 9 HZ) and loading cycles by fitting formulas. The fatigue damage of the OWE pile does not occurs with low frequencies in high cycles.
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http://dx.doi.org/10.3390/s20185348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570637PMC
September 2020

Melatonin inhibits oxalate-induced endoplasmic reticulum stress and apoptosis in HK-2 cells by activating the AMPK pathway.

Cell Cycle 2020 Oct 23;19(20):2600-2610. Epub 2020 Aug 23.

Department of Urology, The First Affiliated Hospital of Zhengzhou University , Zhengzhou, Henan, People's Republic of China.

Deposition of various crystal and organic substances in the kidney can lead to kidney stone formation. Melatonin is an effective endogenous antioxidant that can prevent crystalluria and kidney damage due to crystal formation and aggregation. In this study, we investigated the mechanism by which melatonin inhibits endoplasmic reticulum (ER) stress and apoptosis. We treated HK-2 cells with oxalate to establish an kidney stone model, and treated these cells with different concentrations of melatonin (0, 5, 10, 20 μmol/L) and the AMP-activated protein kinase (AMPK) inhibitor Compound C. We measured levels of stress response markers including reactive oxygen species (ROS), lactate dehydrogenase (LDH), glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), and factors in the stress response pathway, such as ATF6, GRP78, DDIT3, PERK, p-PERK, IRE1, p-IRE1, XBP1s, AMPK, and p-AMPK, using real time-PCR, western blot, and immunofluorescence analyzes. We measured mitochondrial membrane potential and caspases-3 activity using the CCK8, enzyme-linked immunosorbent, and flow cytometry assays to assess HK-2 cell viability and apoptosis. Melatonin improved the total antioxidant capacity (T-AOC) of the HK-2 cells, as evidenced by the dose-dependent reduction in apoptosis, ROS levels, and protein expression of ATF6, GRP78, DDIT3, p-PERK, p-IRE1, XBP1s, caspase-12, cleaved caspase-3 and cleaved caspase-9. Addition of the AMPK inhibitor, Compound C, partially reversed the protective effect of melatonin. Our study revealed that the protective effects of melatonin on oxalate-induced ER stress and apoptosis is partly dependent on AMPK activation in HK-2 cells. These findings provide insight into the prevention and treatment of kidney stones.
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http://dx.doi.org/10.1080/15384101.2020.1810401DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7644152PMC
October 2020

Anaerobic biodegradation of acetochlor by acclimated sludge and its anaerobic catabolic pathway.

Sci Total Environ 2020 Dec 24;748:141122. Epub 2020 Jul 24.

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address:

Acetochlor is a chloroacetamide herbicide that has been widely used for weed control in recent decades. The contamination from its residue in the environment has raised major serious concerns. The aerobic degradation of acetochlor has been well studied; however, little is known regarding its anaerobic degradation. In the study, anaerobic sludge with high acetochlor degradation efficiency was obtained by pressure acclimation in a continuous flow anaerobic reactor. The acetochlor degradation dynamics followed a first-order kinetic reaction equation. The acclimated sludge could degrade six chloroacetamide herbicides with the degradation efficiencies observed as alachlor > acetochlor > propisochlor > butachlor > pretilachlor > metolachlor, and the N-alkoxyalkyl structure of these herbicides significantly affected their biodegradability. Five metabolites, 2-ethyl-6-methyl-N-(ethoxymethyl)-acetanilide, N-(2-methyl-6-ethylphenyl) acetamide, N-2-ethylphenyl acetamide, N-2-ethylphenyl formamide and 2-ethyl-N-carboxyl aniline were identified, and a putative anaerobic acetochlor degradation pathway, initiated by dechlorination, was subsequently proposed. During acclimation, the community diversity of both eubacteria and archaea in the anaerobic sludge decreased, while the abundance of microbes belonging to genera Sporomusa, Sporobacterium, Dechloromonas, Azotobacter and Methanobacterium were significantly increased and dominated the acclimated sludge, and showing a positive correlation with the acetochlor degradation capacity. These findings should be valuable to elucidate the mechanisms associated with the anaerobic catabolism of acetochlor and facilitate the engineering application of anaerobic treatment for removing acetochlor from wastewater.
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http://dx.doi.org/10.1016/j.scitotenv.2020.141122DOI Listing
December 2020

Strain Tunable Semimetal-Topological-Insulator Transition in Monolayer 1T^{'}-WTe_{2}.

Phys Rev Lett 2020 Jul;125(4):046801

Key Laboratory of Artificial Structures and Quantum Control (Ministry of Education), Shenyang National Laboratory for Materials Science, School of Physics and Astronomy, Shanghai Jiao Tong University, Shanghai 200240, China.

A quantum spin hall insulator is manifested by its conducting edge channels that originate from the nontrivial topology of the insulating bulk states. Monolayer 1T^{'}-WTe_{2} exhibits this quantized edge conductance in transport measurements, but because of its semimetallic nature, the coherence length is restricted to around 100 nm. To overcome this restriction, we propose a strain engineering technique to tune the electronic structure, where either a compressive strain along the a axis or a tensile strain along the b axis can drive 1T^{'}-WTe_{2} into an full gap insulating phase. A combined study of molecular beam epitaxy and in situ scanning tunneling microscopy or spectroscopy then confirmed such a phase transition. Meanwhile, the topological edge states were found to be very robust in the presence of strain.
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http://dx.doi.org/10.1103/PhysRevLett.125.046801DOI Listing
July 2020

Two monolayers is greater than a bilayer.

Authors:
Junwei Liu

Nat Mater 2020 08;19(8):824-826

Department of Physics, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.

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http://dx.doi.org/10.1038/s41563-020-0733-5DOI Listing
August 2020

Identification of potential crucial genes and key pathways in osteosarcoma.

Hereditas 2020 Jul 14;157(1):29. Epub 2020 Jul 14.

Department of Radiation Oncology, Chongqing University Cancer Hospital, No. 181, Hanyu road, Shapingba District, Chongqing, 400030, PR China.

Background: The aim of this study is to identify the potential pathogenic and metastasis-related differentially expressed genes (DEGs) in osteosarcoma through bioinformatic analysis of Gene Expression Omnibus (GEO) database.

Results: Gene expression profiles of GSE14359, GSE16088, and GSE33383, in total 112 osteosarcoma tissue samples and 7 osteoblasts, were analyzed. Seventy-four normal-primary DEGs (NPDEGs) and 764 primary-metastatic DEGs (PMDEGs) were screened. VAMP8, A2M, HLA-DRA, SPARCL1, HLA-DQA1, APOC1 and AQP1 were identified continuously upregulating during the oncogenesis and metastasis of osteosarcoma. The enriched functions and pathways of NPDEGs include procession and presentation of antigens, activation of MHC class II receptors and phagocytosis. The enriched functions and pathways of PMDEGs include mitotic nuclear division, cell adhesion molecules (CAMs) and focal adhesion. With protein-protein interaction (PPI) network analyzed by Molecular Complex Detection (MCODE) plug-in of Cytoscape software, one hub NPDEG (HLA-DRA) and 7 hub PMDEGs (CDK1, CDK20, CCNB1, MTIF2, MRPS7, VEGFA and EGF) were eventually selected, and the most significant pathways in NPDEGs module and PMDEGs module were enriched in the procession and presentation of exogenous peptide antigen via MHC class II and the nuclear division, respectively.

Conclusions: By integrated bioinformatic analysis, numerous DEGs related to osteosarcoma were screened, and the hub DEGs identified in this study are possibly part of the potential biomarkers for osteosarcoma. However, further experimental studies are still necessary to elucidate the biological function and mechanism of these genes.
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http://dx.doi.org/10.1186/s41065-020-00142-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7362476PMC
July 2020

The role of significantly deregulated MicroRNAs in osteosarcoma based on bioinformatic analysis.

Technol Health Care 2020 Jun 19. Epub 2020 Jun 19.

Department of Radiation Oncology, Chongqing University Cancer Hospital, Chongqing, 400030, China.

Objective: This study aimed to identify potential key microRNAs (miRNAs) in osteosarcoma and construct miRNA-mRNA negative regulatory networks through analysis of the Gene Expression Omnibus (GEO) database.

Methods: The differentially expressed miRNAs (DE-miRNAs) in GSE28423 were screened, and their prognostic value was assessed with the prognostic data of GSE39058. The target genes of prognostic DE-miRNAs were predicted and underwent Gene Ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. In addition, the expression of all predicted target genes were assessed using the mRNA array data of GSE28424. Finally, the gene-drug interaction network was constructed.

Results: We identified 205 DE-miRNAs between osteosarcoma cells and normal bone. Among them, high expression of miR-411-3p and miR-487b-5p were correlated with prolonged survival. Furthermore, 2659 genes predicted as targets of miR-411-3p or miR-487b-5p were clustered in 42 significant GO categories, including "regulation of neurotransmitter secretion" and "phosphoprotein binding", as well as 23 significant KEGG pathways, such as "MAPK signaling pathway" and "Ras signaling pathway". Five of the 75 overlapping target genes of miR-411-3p and miR-487b-5p were downregulated in osteosarcoma, including ZBTB20, ADAMTS4, GLIPR2, CLIC5 and CBX7.

Conclusions: Our findings might help clarify molecular mechanisms underlying the oncogenesis and development, and offer potential targets for osteosarcoma.
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http://dx.doi.org/10.3233/THC-202138DOI Listing
June 2020

Lasiodiplodia theobromae-induced alteration in ROS metabolism and its relation to gummosis development in Prunus persica.

Plant Physiol Biochem 2020 Sep 22;154:43-53. Epub 2020 May 22.

Key Laboratory of Horticultural Plant Biology-Ministry of Education, College of Horticulture and Forest Sciences, Huazhong Agricultural University, Wuhan, 430070, Hubei Province, China. Electronic address:

Peach gummosis caused by Lasiodiplodia theobromae is one of the most detrimental diseases to peaches in southern China. Reactive oxygen species (ROS) play major roles in plant-pathogen interactions, however, their roles in the pathogenesis of peach gummosis, especially shoot disease in perennials, are largely unknown. In this study, the effects of L. theobromae on ROS production-scavenging systems and on signalling transduction during L. theobromae-induced gummosis in current-year peach shoots were investigated. The infection by L. theobromae led to a ROS burst and activated the plant antioxidant enzyme-dependent scavenging system. With disease progression, the capacity of the plant antioxidant machinery declined, and allowed for ROS accumulation and eventual malondialdehyde production. As for the fungus L. theobromae, the transcripts of genes related to ROS production were significantly repressed, and concomitantly the expression of genes related to antioxidant systems and oxidative stress resistance was markedly upregulated, perhaps to alleviate oxidative stress for successful colonisation. Moreover, genes involved in phytohormones biosynthesis and pathogenesis-related proteins were all markedly promoted, which could contribute to the restriction of disease development in peach shoots. Overall, the results showed that the ROS production-scavenging system in P. persica might affect disease development during peach-L. theobromae interaction. Our findings lay the foundations for future in-depth investigations of the molecular mechanisms and regulatory networks underlying L. theobromae-mediated shoot diseases in terms of ROS production-scavenging systems.
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http://dx.doi.org/10.1016/j.plaphy.2020.05.018DOI Listing
September 2020

A New Approach to Explore the Surface Profile of Clay Soil Using White Light Interferometry.

Sensors (Basel) 2020 May 26;20(11). Epub 2020 May 26.

School of Civil Engineering, Qingdao University of Technology, Qingdao 266033, China.

In order to have a better understanding of the real contact area of granular materials, the white light interference method is applied to explore the real surface morphology of clay soils under high stress. Analysis of the surface profile indicates that there exists a support point height with the highest distribution frequency. A concept of a real contact region (from to + ; represents the particle size corresponding to 90% of the volume fraction) is proposed by combining a surface profile with the particle size distribution of clay soil. It was found that under the compressive stress of 106 MPa-529 MPa, the actual contact area ratio of clay soil varies between 0.375 and 0.431. This demonstrates an increasing trend with the rise of stress. On the contrary, the apparent porosity decreases with an increasing stress, varying between 0.554 and 0.525. In addition, as the compressive stress increases, the cumulative frequency of apparent profile height (from - to + ) has a concentrated tendency with a limited value of 0.9.
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http://dx.doi.org/10.3390/s20113009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7309148PMC
May 2020

Bead-immobilized Pseudomonas stutzeri Y2 prolongs functions to degrade s-triazine herbicides in industrial wastewater and maize fields.

Sci Total Environ 2020 Aug 5;731:139183. Epub 2020 May 5.

College of Resources and Environment, Anhui Agricultural University, Key Laboratory of Agri-food Safety of Anhui Province, Hefei 230036, China. Electronic address:

Functional durability of bio-augmented microbes in contaminated fields remains a major challenge in bioremediation. In the present study, various immobilization materials and compositional combinations were designed and compared to enhance the functional durability of Pseudomonas stutzeri sp. Y2 for degradation of simazine, one of the most used herbicides, in industrial wastewater and maize fields. Among four combinations of materials tested, the optimal combination obtained from the orthogonal array trials was 14% polyvinyl alcohol (PVA), 1-3% sodium alginate (SA), 2% activated carbon (AC), and 1-2% Y2 cells (PSC-Y2), which yielded 1.7 fold faster degradation of simazine at 50 mg L than that by free Y2 cells in the industrial wastewater. The degradation half-lives (DT) of simazine (10 mg L) by free Y2 cells and PSC-Y2 was 1.1 d and 5.3 d in laboratory soil, respectively. The DT of simazine by PSC-Y2 at the recommended and double dosages of simazine (0.45 and 0.9 g ai·m) was 17.2 d and 12.4 d in the maize fields, respectively, in comparison with 23 d and 17.4 d by free Y2 cells. In addition, the PSC-Y2 degraded 100% of atrazine and terbuthylazine, and 96% of propazine at an initial concentration of 50 mg L each in 4 days. This study provides an immobilization strategy to stabilize bacteria and prolong bacterial functions to treat s-triazine herbicides contaminated water and soil.
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http://dx.doi.org/10.1016/j.scitotenv.2020.139183DOI Listing
August 2020

Rudanella paleaurantiibacter sp. nov., Isolated from Activated Sludge.

Curr Microbiol 2020 Sep 5;77(9):2016-2022. Epub 2020 May 5.

Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.

A Gram-stain-negative, orange-colored bacterium, designated HX-22-17, was isolated from activated sludge of an agricultural chemical plant in Maanshan, Anhui province, China (118° 52' E 31° 68' N). The strain was strictly aerobic, non-endospore forming, non-motile, and ellipse. Growth of the strain was observed at 16-42 °C (optimum between 25 and 30 °C) at pH 6.0-9.0 (optimum at pH 7.0) and with 0-6.0% (w/v) NaCl (optimum at 1.0%). 16S rRNA gene sequence analysis showed that the strain was most closely related to Rudanella lutea KACC 12603 (99.5% similarity). The predominant cellular fatty acids were summed feature 3 (Cω7c and/or Cω6c), iso-C, and anteiso-C. The major polar lipids included posphatidylethanolamine (PE), aminolipid (AL), and phospholipids (PL). The genomic DNA G+C content of the strain was 54.1 mol%. The ANI and dDDH values obtained between the genomes of HX-22-17 and R. lutea KACC 12603 were 89.3% and 39.3%, respectively. The phenotypic, chemotaxonomic, and genotypic data clearly showed that strain HX-22-17 represents a novel species of the genus Rudanella, for which the name R. paleaurantiibacter sp. nov. is proposed. The type strain is HX-22-17 (=KCTC 72656 = CCTCC AB 2019347).
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http://dx.doi.org/10.1007/s00284-020-02005-3DOI Listing
September 2020

Phytoremediation of acetochlor residue by transgenic Arabidopsis expressing the acetochlor N-dealkylase from Sphingomonas wittichii DC-6.

Sci Total Environ 2020 Aug 14;728:138687. Epub 2020 Apr 14.

College of Life Sciences, Zaozhuang University, Zaozhuang 277100, China. Electronic address:

Transgenic engineering is an effective way for plants to obtain strong degradation or detoxification abilities to target pollutants. Acetochlor is an important and widely used herbicide, however, its residue is persistent in soil and is toxic to humans and rotation crops. In this study, the degradation ability and tolerance to acetochlor of transgenic Arabidopsis thaliana synthesizing the oxygenase component, CndA, of the bacterial acetochlor N-dealkylase system, CndABC, were investigated. Two transgenic plants, including a cytoplasm transformant, in which the CndA was located in the cytoplasm, and a chloroplast transformant, in which the CndA was located in the chloroplast, were constructed. The cytoplasm transformant acquired only weak acetochlor degradation activity and displayed little acetochlor tolerance. In contrast, the chloroplast transformant exhibited high degradation efficiency and strong tolerance to acetochlor; it could transform 94.3% of 20 μM acetochlor in water within 48 h and eliminate 80.2% of 5 mg/kg acetochlor in soil within 30 d. The metabolite of acetochlor N-dealkylation catalyzed by CndA, 2-chloro-N-(2-methyl-6-ethylphenyl)acetamide (CMEPA), could be released outside the cells by chloroplast transformant and further degraded by indigenous microorganisms in the soil. This study provides an effective strategy for the phytoremediation of acetochlor residue in water and soil.
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http://dx.doi.org/10.1016/j.scitotenv.2020.138687DOI Listing
August 2020

Factors affecting people's attitude toward participation in medical research: a systematic review.

Curr Med Res Opin 2020 07 14;36(7):1137-1143. Epub 2020 May 14.

Zhuhai People's Hospital (Zhuhai Hospital Affiliated with Jinan University), Zhuhai, China.

Human participants play an important role in medical care advances. Recruiting enough participants is perhaps the most significant procedure that determines the success of medical research, and high participation rate brings about many benefits. Therefore, acquiring enough participants is important for medical researchers. To understand how to improve participation rate, we need to clarify factors affecting the public's attitude toward medical research. Through this review, we aim to examine which factors affect the attitude of human participants toward medical research. The relevant data were searched by using the keywords "Public," "Participants," "Medical" and "Research" in PubMed (MEDLINE), International Pharmaceutical Abstracts, Web of Science, Science Direct, CINAHL Plus, EMBASE, and China Knowledge Resource Integrated databases. A manual search was done to acquire peer-reviewed articles and reports about participation in medical research. Sixty-three studies were identified for inclusion after full text screening. The included studies were of variable quality. Some factors affecting people's attitude toward participating in medical research have been identified and discussed in our review. This review demonstrated that willingness of participants to take part in medical research was influenced by a variety of factors. These factors may be used to predict the public's willingness to take part in medical research and may potentially be used in developing strategies aimed at improving participation rate.
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http://dx.doi.org/10.1080/03007995.2020.1760807DOI Listing
July 2020

Glycogen metabolism regulates macrophage-mediated acute inflammatory responses.

Nat Commun 2020 04 14;11(1):1769. Epub 2020 Apr 14.

Department of Immunology, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, 430030, China.

Our current understanding of how sugar metabolism affects inflammatory pathways in macrophages is incomplete. Here, we show that glycogen metabolism is an important event that controls macrophage-mediated inflammatory responses. IFN-γ/LPS treatment stimulates macrophages to synthesize glycogen, which is then channeled through glycogenolysis to generate G6P and further through the pentose phosphate pathway to yield abundant NADPH, ensuring high levels of reduced glutathione for inflammatory macrophage survival. Meanwhile, glycogen metabolism also increases UDPG levels and the receptor P2Y in macrophages. The UDPG/P2Y signaling pathway not only upregulates the expression of STAT1 via activating RARβ but also promotes STAT1 phosphorylation by downregulating phosphatase TC45. Blockade of this glycogen metabolic pathway disrupts acute inflammatory responses in multiple mouse models. Glycogen metabolism also regulates inflammatory responses in patients with sepsis. These findings show that glycogen metabolism in macrophages is an important regulator and indicate strategies that might be used to treat acute inflammatory diseases.
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http://dx.doi.org/10.1038/s41467-020-15636-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7156451PMC
April 2020

Complex emergencies of COVID-19: management and experience in Zhuhai, China.

Int J Antimicrob Agents 2020 May 28;55(5):105961. Epub 2020 Mar 28.

Vice-president, Zhuhai People's Hospital (Zhuhai hospital affiliated with Jinan University), Zhuhai 519000, China. Electronic address:

The impact of communicable diseases (infectious diseases) on human health is obvious. The sudden outbreak of COVID-19 (Corona Virus Disease 2019) has made people realise the threat of communicable diseases to mankind. As a city of many migrants, Zhuhai Special Economic Zone experienced great challenges brought about by the COVID-19 epidemic. Experience has been acquired from all aspects of this. A highly reactive, multifunctional and efficient emergency management system should be established, and the significance of information communication should be fully understood for the future.
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http://dx.doi.org/10.1016/j.ijantimicag.2020.105961DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7270772PMC
May 2020

Evidence that the second human pegivirus (HPgV-2) is primarily a lymphotropic virus and can replicate independent of HCV replication.

Emerg Microbes Infect 2020 26;9(1):485-495. Epub 2020 Feb 26.

Department of Epidemiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, People's Republic of China.

The second human pegivirus HPgV-2 is a novel blood-borne virus that is strongly associated with the hepatitis C virus (HCV) infection. However, the molecular evidence for their association as well as the natural history and tissue tropism of HPgV-2 remain to be elucidated. In this longitudinal study, a total of 753 patients including 512 HIV-1 and HCV co-infected patients were enrolled to characterize the natural history of HPgV-2 infection. Peripheral blood mononuclear cells (PBMCs) and liver biopsies were collected to determine the tissue tropism of HPgV-2 using immunohistochemical staining of the HPgV-2 antigen and in situ hybridization of HPgV-2 RNA. We documented both persistent HPgV-2 infection with the presence of HPgV-2 viral RNA and antibodies up to 4.6 years and resolved HPgV-2 infection, accompanied by a simultaneous decline of anti-HPgV-2 antibodies and clearance of HPgV-2 viremia. Furthermore, we observed the clearance of HCV, but not HPgV-2, by treatment with direct-acting antivirals (DAAs). Biochemical tests and pathological analyses did not reveal any indication of hepatic impairment caused by HPgV-2. HPgV-2 RNA and nonstructural antigen were detected in the lymphocytes, but not in the hepatocytes present in the liver biopsy samples. In addition, both positive- and negative-strand HPgV-2 RNAs were detected in PBMCs, especially in B cells. The present study is the first to provide evidence that HPgV-2 is a lymphotropic, but not a hepatotropic virus and that HPgV-2 replication is independent of HCV viremia. These new findings let us gain insights into the evolution and persistent infection of RNA viruses in humans.
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http://dx.doi.org/10.1080/22221751.2020.1730247DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7054972PMC
March 2020

DNA methylation profiling in recurrent miscarriage.

PeerJ 2020 7;8:e8196. Epub 2020 Jan 7.

NHC Key Lab. of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Medical School, Fudan University, Shanghai, China.

Recurrent miscarriage (RM) is a complex clinical problem. However, specific diagnostic biomarkers and candidate regulatory targets have not yet been identified. To explore RM-related biological markers and processes, we performed a genome-wide DNA methylation analysis using the Illumina Infinium HumanMethylation450 array platform. Methylation variable positions and differentially methylated regions (DMRs) were selected using the Limma package in R language. Thereafter, gene ontology (GO) enrichment analysis and pathway enrichment analysis were performed on these DMRs. A total of 1,799 DMRs were filtered out between patients with RM and healthy pregnant women. The GO terms were mainly related to system development, plasma membrane part, and sequence-specific DNA binding, while the enriched pathways included cell adhesion molecules, type I diabetes mellitus, and ECM-receptor interactions. In addition, genes, including , , , , and , were obtained. These genes may be potential candidates for diagnostic biomarkers and possible regulatory targets in RM. We then detected the mRNA expression levels of the candidate genes. The mRNA expression levels of the candidate genes in the RM group were significantly higher than those in the control group. However, additional research is still required to confirm their potential roles in the occurrence of RM.
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http://dx.doi.org/10.7717/peerj.8196DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953351PMC
January 2020

AFP specificity for HCC surveillance is increased by mitigating liver injury among treated chronic hepatitis B patients with elevated AFP.

Int J Clin Exp Pathol 2019 1;12(4):1315-1323. Epub 2019 Apr 1.

Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital, Southern Medical University Guangzhou, China.

Objective: The aim of this study was to assess AFP response in chronic hepatitis B (CHB) patients with baseline positive AFP (≥7 ng/mL) who received antiviral therapy thereafter.

Methods: A cohort study was conducted to assess AFP response in CHB patients who had baseline positive AFP and got antiviral therapy.

Results: This retrospective study enrolled 302 antiviral-treatment-naïve CHB patients with positive AFP. After a 12-month antiviral treatment, 144 patients normalized AFP during follow-up while the rest remained AFP-positive. There were no significant differences in baseline characteristics and virologic and ALT responses to antiviral therapy between the two groups. During a mean follow-up of 34 ± 6 months, 16 patients (5.3%) in this cohort developed HCC, and 14 (8.9%) of them emerged in the AFP positive group. There was a significant difference (=0.004) in HCC occurrence between AFP normalized and non-normalized groups after treatment. Univariate and multivariate analyses revealed that cirrhosis (HR=9.983, 95% CI=3.609-27.617, <0.001), and non-AFP response to antiviral treatment (HR=6.517, 95% CI=1.475-28.784, =0.013) were two independent factors associated with HCC occurrence.

Conclusions: To our knowledge, this is the first investigator-initiated cohort study to assess the performance of on-treatment AFP in CHB patients with baseline positive AFP. In contrast to the criticism that AFP is neither sensitive nor specific, the current study has provided important evidence that on-antiviral-treatment AFP normalization is a specific protective marker for HCC in patients with HBV-related chronic liver diseases who started antiviral therapy thereafter.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947068PMC
April 2019

[Incidence and characteristics of benign liver space-occupying mass in 17 721 patients with chronic hepatitis B: a color Doppler ultrasound-based case-control study].

Nan Fang Yi Ke Da Xue Xue Bao 2019 Oct;39(10):1149-1154

Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Objective: To analyze the incidence and risk factors of benign liver space-occupying mass in patients with chronic hepatitis B (CHB) and the ultrasound features that differentiate these masses from small hepatocellular carcinoma.

Methods: We retrospectively analyzed the color Doppler and clinical data of 17 721 patients with CHB treated in the Hepatology Unit of Nanfang Hospital between January, 2016 and December, 2017. The data were compared with those of 21629 healthy control subjects undergoing routine physical examination in the Center of Heath Management of Nanfang Hospital during the same period.

Results: Compared with the control subjects, the patients with CHB had significantly higher incidences of hepatic cysts (11.8% 8.7%, < 0.05), hepatic hemangioma (8.2% 1.6%, < 0.05) and hepatic cirrhosis nodules (20.6% 2.4%, < 0.05). The incidences of hepatic cysts and cirrhosis nodules increased with age and was significantly higher in male than in female patients ( < 0.001). The highest incidence of hepatic hemangioma was found in CHB patients aged 30-49 years without a gender difference (>0.05). Sonographically, the benign liver masses commonly showed homogeneous echo within the lesion with clear boundaries and regular shape. Hepatic hemangioma was distinctively hyperechoic in 83.32% (1579/1895) of the patients, while small hepatocellular carcinoma presented with weaker peripheral and internal blood flow signals with a lower flow velocity in the arteries and a higher flow velocity in the portal vein. Liver cirrhosis nodules mostly showed a mixture of strong and weak echoes (79.60%; 7637/9595) without blood flow signal within or around the nodule; an increased volume of the nodule accompanied by heterogeneous echoes within the nodule indicated an increased probability of malignant lesion. Hepatic cysts often displayed no echo within the lesion, but the echo could be enhanced posteriorly.

Conclusions: The patients with CHB are at a significantly higher risk of developing hepatic cysts, hepatic hemangiomas and hepatic cirrhosis nodules than the control population, and an older age and the male gender are associated with a higher incidence of hepatic cysts or cirrhosis. The differences in the sonographic and hemodynamic features can help to differentiate hepatic benign mass from malignant lesions, and kinetic changes in sonography can be used to monitor potential malignant transformation of the cirrhotic lesions.
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http://dx.doi.org/10.12122/j.issn.1673-4254.2019.10.03DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6867945PMC
October 2019