Publications by authors named "Junqin Li"

75 Publications

The effects of human dermal-derived mesenchymal stem cells on the keratinocyte proliferation and apoptosis in psoriasis.

Exp Dermatol 2021 Apr 10. Epub 2021 Apr 10.

Shanxi Key Laboratory of Stem Cell for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, China.

Psoriasis is a common chronic inflammatory skin disease, characterized by epidermal hyperproliferation. Mesenchymal stem cells (MSCs) regulate inflammation and vascular proliferation in the psoriasis lesions. Whether dermal-derived mesenchymal stem cells (DMSCs), the main MSCs in the dermis, regulate keratinocyte proliferation and apoptosis remains unknown. In the present study, we assessed the proliferation and apoptosis of keratinocytes cocultured with DMSCs isolated from either normal or psoriatic involved skin. Cell growth and apoptotic rates were determined using Cell Count Kit-8 and annexin V-FITC staining, respectively. In addition, EDU kit was also used to measure the rate of keratinocyte proliferation. Our results showed that psoriatic DMSCs (pDMSCs) were more potent than normal DMSCs (nDMSCs) in stimulating keratinocyte proliferation. In contrast, the apoptotic rate and expression levels of caspase-3 protein were lower in pDMSC-treated than nDMSC-treated keratinocytes (p < 0.001). Moreover, significantly higher contents of IL-6, IL-8, TNF-α and IFN-γ were found in the culture medium of pDMSCs than in that of nDMSCs. In conclusion, pDMSCs were more potent than nDMSCs in stimulation of keratinocyte proliferation and secretion of proinflammatory cytokines, but weaker in promoting apoptosis.
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http://dx.doi.org/10.1111/exd.14353DOI Listing
April 2021

Agromyces laixinhei sp. nov. isolated from bat feces in China.

J Microbiol 2021 May 29;59(5):467-475. Epub 2021 Mar 29.

Department of Epidemiology, Shanxi Medical University School of Public Health, Taiyuan, Shanxi, 030001, China.

Three rod-shaped, Gram-stain-positive, and catalase-positive, phenotypically closely related isolates (HY052, HY050, and HY045) were obtained from fecal samples collected from bats in Guangxi province and Chongqing city of China. Circular, smooth, light-yellow colonies appeared on brain heart infusion plate after 24-48 h incubation at 28°C. The optimal pH for growth was between 6.0 and 7.5. Based on 16S rRNA, the three isolates were phylogenetically related to Agromyces terreus DS-10, Agromyces aureus AR33, Agromyces salentinus 20-5, Agromyces allii UMS-62, Agromyces lapidis CD55, and Agromyces italicus CD1. Moreover, based on 296 core genes, the phylogenomic tree indicated that the three isolates clustered together, closest to Agromyces cerinus VKM Ac-1340 and Agromyces fucosus VKM Ac-1345 but separated distantly from other Agromyces species. The average nucleotide identity values between strain HY052 and other Agromyces species ranged from 79.3% to 87.9%, lower than the 95-96% threshold. Furthermore, the genome of strain HY052 contains a circular chromosome of 3,437,203 bp with G + C content of 69.0 mol%. Main fatty acids were anteiso-C and anteiso-C. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, and unidentified glycolipids. Rhamnose, ribose, and glucose were the primary cell wall sugars. The major peptidoglycan amino acids included alanine, glutamic acid, glycine, and 2,4-diaminobutyric acid. An additional remarkable difference from other Agromyces species is that MK-12 was the sole menaquinone in strain HY052. Based on results from the polyphasic characterizations performed in this study, our isolates are proposed to be members of a novel species in genus Agromyces, named Agromyces laixinhei. The type strain is HY052 (= CGMCC 1.17175 = JCM 33695).
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http://dx.doi.org/10.1007/s12275-021-0546-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8006105PMC
May 2021

Increased angiogenesis and migration of dermal microvascular endothelial cells from patients with psoriasis.

Exp Dermatol 2021 Mar 22. Epub 2021 Mar 22.

Shanxi Key Laboratory of Stem Cell for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, China.

Psoriasis displays both increased angiogenesis and microvascular dilation in the skin, while human dermal microvascular endothelial cells (HDMECs) are involved in angiogenesis and microvascular dilation. Whether the functions of HDMECs are altered in psoriatic skin versus healthy skin remain unknown. Here, we isolated HDMECs from the skin of 10 patients with psoriasis and 10 healthy subjects and compared angiogenesis, proliferation, migration and cell metabolism between psoriatic HDMECs and normal HDMECs. We found that the morphology of primary HDMECs was comparable between psoriatic HDMECs and normal HDMECs. After passage, psoriatic HDMECs displayed larger cell size and wider intercellular space. In addition to DiI-Ac-LDL (DiI-labelled acetylated low-density lipoprotein) uptake, expression levels of CD31, vWF (von Willebrand factor) and LYVE-1 were comparable in psoriatic HDMECs versus normal HDMECs. However, psoriatic HDMECs exhibited increased tube formation (numbers of nodes and meshes, p < 0.05) and migration (numbers of migrated cells, p < 0.001) and reductions in proliferation (growth rates, p < 0.05) and energy metabolism (oxygen consumption rate and extracellular acidification rate, p < 0.05) compared with normal HDMECs. Therefore, psoriatic HDMECs display an increased angiogenesis and migration and decreased proliferation and metabolic activity, suggesting a pathogenic role of HDMECs in psoriasis.
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http://dx.doi.org/10.1111/exd.14329DOI Listing
March 2021

Psoriatic mesenchymal stem cells stimulate the angiogenesis of human umbilical vein endothelial cells in vitro.

Microvasc Res 2021 Mar 1;136:104151. Epub 2021 Mar 1.

Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, No. 5 Dong San Dao Xiang, Jiefang Road, Taiyuan 030009, China. Electronic address:

Objective: To investigate the regulation of psoriatic dermal mesenchymal stem cells (p-DMSCs) in the expression of vascular growth factor (VEGF), and migration and angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro.

Methods: A co-culture model of HUVECs and dermal mesenchymal stem cells (DMSCs)was used in this study. After 7-day co-culture, changes in expression levels of VEGF mRNA and protein in HUVECs were assessed using RT-PCR and Western Blotting, respectively. Migration and tubular formation of HUVECs were also assessed following co-culture of DMSCs and HUVECs.

Results: In comparison to either HUVECs alone or co-culture of n-DMSCs and HUVECs, co-culture of HUVECs and p-DMSCs significantly increased expression levels of both VEGF mRNA (p < 0.01 vs. HUVECs alone) and protein in HUVECs (p < 0.001 vs. both HUVECs alone and HUVECs co-cultured with n-DMSCs). Moreover, p-DMSCs stimulated HUVEC migration and vascular formation (p < 0.05 vs. both HUVECs alone and co-culture of n-DMSCs and HUVECs).

Conclusion: Psoriatic DMSCs can upregulate VEGF expression, and stimulate migration and angiogenesis of HUVECs, suggesting a pathogenic role of p-DMSCs in psoriasis.
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http://dx.doi.org/10.1016/j.mvr.2021.104151DOI Listing
March 2021

Dysregulated Dermal Mesenchymal Stem Cell Proliferation and Differentiation Interfered by Glucose Metabolism in Psoriasis.

Int J Stem Cells 2021 Feb;14(1):85-93

Shanxi Key Laboratory of Stem Cell for Immunological Dermatosis, Institute of Dermatology, Taiyuan City Centre Hospital of Shanxi Medical University, Taiyuan, China.

Background And Objectives: Psoriasis is a chronic inflammatory skin disease, which the mechanisms behind its initiation and development are related to many factors. DMSCs (dermal mesenchymal stem cells) represent an important member of the skin microenvironment and play an important role in the surrounding environment and in neighbouring cells, but they are also affected by the microenvironment. We studied the glucose metabolism of DMSCs in psoriasis patients and a control group to reveal the relationship among glucose metabolism, cell proliferation activity,and VEC (vascular endothelial cell) differentiation , we demonstrated the biological activity and molecular mechanisms of DMSCs in psoriasis.

Methods And Results: We found that the OCR of DMSCs in psoriatic lesions was higher than that in the control group, and mRNA of GLUT1 and HK2 were up-regulated compared with the control group. The proliferative activity of DMSCs in psoriasis was reduced at an early stage, and mRNA involved in proliferation, JUNB and FOS were expressed at lower levels than those in the control group. The number of blood vessels in psoriatic lesions was significantly higher than that in the control group (p<0.05), which the mRNA of VEC differentiation, CXCL12, CXCR7, HEYL and RGS5 tended to be increased in psoriatic lesions compared to the control group, in addition to Notch3.

Conclusions: We speculated that DMSCs affected local psoriatic blood vessels through glucose metabolism, and the differentiation of VECs, which resulted in the pathophysiological process of psoriasis.
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http://dx.doi.org/10.15283/ijsc20073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904530PMC
February 2021

sp. nov. and sp. nov., isolated from plateau pika () on the Qinghai-Tibet Plateau of PR China.

Int J Syst Evol Microbiol 2021 Feb;71(2)

Shanghai Institute for Emerging and Re-emerging Infectious Diseases, Shanghai Public Health Clinical Center, Shanghai 201508, PR China.

Four novel bacterial strains (ST-M6, L-033, L-031 and Z-333) were isolated from the intestinal contents of plateau pikas () collected on the QinghaiTibet Plateau, PR China. Cells were aerobic, non-motile, Gram-stain-positive, catalase-positive, oxidase-negative, capsuled and short-rod-shaped. Phylogenetic analyses based on the 16S rRNA gene sequences and 387 core genes indicated that the four isolates belong in the genus and clearly separate from recognized species. The two type strains (ST-M6 and L-031) shared low 16S rRNA similarity, average nucleotide identity values and digital DNA-DNA hybridization relatedness with their phylogenetic neighbours ( DSM 18659, DSM 19179, JCM 30598, CCTCC M208212, DSM 16091 and DSM 20754). The genomic DNA G+C contents of strains ST-M6 and L-031 were 70.4 and 70.7 mol%, respectively. The major cellular fatty acids of strain ST-M6 were anteiso-C, anteiso-C and iso-C, in contrast to anteiso-C, anteiso-C and anteiso-C9 of strain L-031. Both type strains (ST-M6 and L-031) were glycolate test positive and shared the following common features: MK-11 and MK-12 as major menaquinones; rhamnose, ribose, mannose and galactose as major cell-wall sugars; diphosphatidylglycerol, phosphatidylglycerol and two glycolipids as polar lipids; and ornithine, alanine, glycine and glutamic acid as cell-wall amino acids. Comparing the phenotypic, phylogenetic and chemotaxonomic features of the four strains and their related taxa, strains ST-M6 and L-031 represent two novel species of the genus , for which the names sp. nov. (type strain ST-M6=CGMCC 1.16364=DSM 104058) and sp. nov. (type strain L-031 =CGMCC 1.16363=DSM 106170) are proposed.
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http://dx.doi.org/10.1099/ijsem.0.004662DOI Listing
February 2021

Effect of Hypothermia on Serum Myelin Basic Protein and Tumor Necrosis Factor-α in Neonatal Hypoxic-Ischemic Encephalopathy.

Am J Perinatol 2021 Jan 17. Epub 2021 Jan 17.

Department of Neonatal Pathology, Handan Maternal and Child Health Care Hospital, Handan, Hebei Province, People's Republic of China.

Objective:  Multiple randomized controlled trials have shown that hypothermia is a safe and effective treatment for neonatal moderate or severe hypoxic-ischemic encephalopathy (HIE). The neuroprotective mechanisms of hypothermia need further study. The aim of this study was to investigate the effect of hypothermia on the serum levels of myelin basic protein (MBP) and tumor necrosis factor-α (TNF-α) as well as neurodevelopmental outcomes in neonatal HIE.

Study Design:  Eighty-five neonates with moderate-to-severe HIE were divided into a hypothermia group ( = 49) and a control group ( = 36). Serum levels of MBP and TNF-α within 6 hours after birth and after 3 days of treatment were determined by enzyme-linked immunosorbent assay, and neurodevelopmental outcome at the age of 12 to 15 months was assessed by using the Gesell development scale.

Results:  After 3 days of treatment, serum levels of MBP and TNF-α in the control group were not significantly different from levels before treatment ( > 0.05), and serum levels of MBP and TNF-α in the hypothermia group were significantly lower than levels before treatment ( < 0.05). Serum levels of MBP and TNF-α were significantly negatively correlated with developmental quotient (DQ;  =  - 0.7945,  = 0.0000;  =  - 0.7035,  = 0.0000, respectively). Serum levels of MBP and TNF-α in neurodevelopmentally impaired infants were significantly higher than those in infants with suspected neurodevelopmental impairment and those in neurodevelopmentally normal infants (both < 0.01). The rate of reduction of neurodevelopmental impairment was higher among infants in the hypothermia group than among those in the control group (χ = 16.3900,  < 0.05).

Conclusion:  Hypothermia can reduce serum levels of MBP and TNF-α in neonates with HIE. Inhibiting the release of TNF-α may be one of the mechanisms by which hypothermia protects the myelin sheath.

Key Points: · Hypothermia can reduce serum levels of MBP and TNF-α in neonatal HIE.. · Hypothermia improves neurodevelopmental outcomes and reduces the rate of neurodevelopmental impairment.. · Hypothermia is a feasible and effective treatment for neonates with moderate or severe HIE..
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http://dx.doi.org/10.1055/s-0040-1722601DOI Listing
January 2021

Phase analysis for partially coherent light propagating through an optimized aperture in a synchrotron beamline.

J Synchrotron Radiat 2020 Nov 14;27(Pt 6):1485-1493. Epub 2020 Sep 14.

University of Chinese Academy of Sciences, Yuquan Road 19, Shijingshan District, Beijing 100049, People's Republic of China.

The mutual optical intensity propagation of partially coherent light through a beamline is calculated for different aperture sizes and positions. The coherence, intensity and phase distribution can be extracted from the mutual optical intensity. The phase distribution depends on the aperture size and position. The results show that the widest flat phase distribution is obtained at the optimized aperture size and position. The aperture plays a more important role for partially coherent light than for incoherent light. The influence of the aperture size and position on the intensity and spot size at the focal plane is also analyzed. A way to obtain a balance between the flat phase distribution area, spot size and intensity for partially coherent light in the beamline is demonstrated.
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http://dx.doi.org/10.1107/S1600577520010565DOI Listing
November 2020

Dermal mesenchymal stem cells promoted adhesion and migration of endothelial cells by integrin in psoriasis.

Cell Biol Int 2021 Feb 8;45(2):358-367. Epub 2020 Nov 8.

Dermatology Department, The First Hospital of China Medical University, Shenyang, Liaoning, China.

The unusual dilatation of dermal capillaries and angiogenesis played important roles in psoriasis. Some genes and proteins of dermal mesenchymal stem cells (DMSCs) from psoriasis are abnormal and related to the function of endothelial cells (ECs). The present study was aimed to evaluate whether psoriatic DMSCs could affect adhesion and migration of ECs through neovascularization-related integrins in psoriasis. Human DMSCs, collected from psoriasis lesions and healthy skin, respectively, were cocultured with human umbilical vein endothelial cells (HUVECs). The expression levels of three integrins, that is, αvβ3, αvβ5, and α5β1 in HUVECs were tested by quantitative real-time polymerase chain reaction and Western blot analysis. The adhesion and migration of HUVECs were detected by adhesion assay and migration assay. The results showed that in psoriasis group, the expression of αVβ3 and α5β1 of HUVECs markedly increased 2.50- and 3.71-fold in messenger RNA levels, and significantly increased 1.63- and 1.92-fold in protein levels, comparing to healthy control group (all p < .05). But β5 was not significantly different between the two groups (p > .05). In addition, compared with control, psoriatic DMSCs promoted HUVECs adhesion by 1.62-fold and migration by 2.91-fold (all p < .05). In conclusion, psoriatic DMSCs impact HUVECs adhesion and migration by upregulating the expression of integrins αVβ3 and α5β1.
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http://dx.doi.org/10.1002/cbin.11492DOI Listing
February 2021

The regulation of dermal mesenchymal stem cells on keratinocytes apoptosis.

Cell Tissue Bank 2021 Mar 29;22(1):57-65. Epub 2020 Sep 29.

Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, No. 5 East Third Lane, Jiefang Road, Taiyuan, 030009, Shanxi, China.

Dermal mesenchymal stem cells (DMSCs) are progenitor cells with the capacity of self-renewal, multilineage differentiation, and immunomodulation, which were reported to induce the proliferation of keratinocytes, however the regulation on keratinocytes apoptosis was unknown. In this study, we isolated DMSCs from normal skin and co-cultured with keratinocytes, and then detected apoptosis of keratinocytes by flow cytometry and expression of apoptosis associated proteins by western blot. The mRNA expression profile of normal DMSCs was investigated by RNA sequencing. The results of our study presented that the DMSCs promoted HaCaT cells apoptosis both in early apoptotic state (13.8 vs. 2.9, p < 0.05) and late apoptotic state (4.2 vs. 0.7, p < 0.05). The expression of apoptosis associated proteins caspase-3 (3.51 vs. 1.99, p < 0.05) and lymphoid enhancer-binding factor 1 (3.10 vs. 0.83, p < 0.05) were upregulated. However, the cell cycle protein cyclin E1 was similar (9.38 vs. 9.05, p > 0.05). Moreover, 33 genes with the function of induced cell apoptosis were highly expressed in DMSCs, including insulin-like growth factor-binding protein 4 (2828.13), IGFBP7 (1805.69), cathepsin D (1694.34), cathepsin B (CTSB, 1641.40) and dickkopf WNT signaling pathway inhibitor 1 (DKK1, 384.79). This study suggested DMSCs induce the apoptosis of keratinocytes through non-G1/S phase blockade via highly expression of apoptosis inducer.
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http://dx.doi.org/10.1007/s10561-020-09865-wDOI Listing
March 2021

Enhanced Interfacial Reliability and Mechanical Strength of CoSb-Based Thermoelectric Joints with Rationally Designed Diffusion Barrier Materials of Ti-Based Alloys.

ACS Appl Mater Interfaces 2020 Oct 25;12(40):44858-44865. Epub 2020 Sep 25.

College of Materials Sciences and Engineering, Shenzhen Key Laboratory of Special Functional Materials; Shenzhen Engineering Laboratory for Advanced Technology of Ceramics and Guangdong Research Center for Interfacial Engineering of Functional Materials, Shenzhen University, Shenzhen 518060, P. R. China.

To achieve high-performance thermoelectric (TE) devices, constructing a good interfacial connection between TE materials and electrodes is as important as having high figure-of-merit TE materials. Although CoSb-based TE devices have received great attention for power generation recently, the limited long-term service stability is the main obstruct for their applications. In this work, we have prepared two kinds of Ti-based alloys (TiAlSi and TiNi) as the diffusion barrier layer of CoSb-based TE joints by the spark plasma sintering method and have systematically investigated their interfacial behaviors during the aging process. The performances of contact resistivity and mechanical strength for TiNi/YbCoFeSb TE joints are good before aging treatment but gradually deteriorate during the aging process, which should be ascribed to the phase-transition-induced negative thermal expansion in Ti-Ni alloys. On the other hand, TiAlSi/YbCoFeSb TE joints show both low contact resistivity (<10 μΩ·cm) and high mechanical strength (>20 MPa) before and after 16-day aging at 500 °C, which is originated from the matching of the coefficient of thermal expansion (CTE) and the formation of network structures in Ti-Al-Si alloys. We have also prepared an eight-couple TE module of p-GeSbTeB/n-YbCoFeSb and have measured its corresponding device performance. Our work has demonstrated that the matched CTE and network structures in the Ti-Al-Si alloy are key to obtain high-performance CoSb-based TE joints for long-term service.
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http://dx.doi.org/10.1021/acsami.0c14180DOI Listing
October 2020

An effective method of isolating microvascular endothelial cells from the human dermis.

Cell Biol Int 2020 Dec 31;44(12):2588-2597. Epub 2020 Aug 31.

Shanxi Key Laboratory of Stem Cell for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, Shanxi, China.

Dermal microvascular endothelial cells (DMECs) play central roles in inflammation and angiogenesis and have become important cell models for studying various skin diseases. However, primary DMECs are difficult to culture and often contaminated by mesenchymal stem cells, fibroblasts, and other stromal cells. Surgically removed superfluous foreskin was first cut into pieces, digested with two types of enzymes, and dispersed into single cells. Cells obtained from the dermis were then subjected to Percoll density gradient centrifugation and cells located between densities 1.033 and 1.047 g/ml were further purified with endothelial growth medium containing decreasing concentrations of puromycin. Obtained HDMECs were identified by microscopy, flow cytometry, quantitative reverse-transcription polymerase chain reaction, western blot analysis, and immunofluorescent staining. The expression of CD31 (PECAM-1), CD34, VEGFR2, VWF (Von Willebrand Factor), VE-Cadherin (CD144), and NOS was positive. HDMECs were found to have abilities of angiogenesis and uptake of acetylated low-density lipoprotein. Growth curves and cell viability were analyzed, and a growth pattern consisting of the "latency phase-logarithmic growth phase-stagnation phase" was determined. In this study, a simple, rapid, effective, and low-cost method is established to isolate HDMECs from the foreskin with a purity of over 91% and high viability. The method showed good repeatability and allowed a stable passage. This study provides technical support and theoretical guidance for studying the physiological characteristics of HDMECs, the pathogenesis of the skin associated, and other microvascular diseases.
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http://dx.doi.org/10.1002/cbin.11448DOI Listing
December 2020

Psoriasis-associated angiogenesis is mediated by EDIL3.

Microvasc Res 2020 11 12;132:104056. Epub 2020 Aug 12.

Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China. Electronic address:

The dermal mesenchymal stem cells (DMSCs) from psoriasis display higher expression level of epidermal growth factor-like repeats and discoidin I-like domains 3 (EDIL3), while EDIL3 can bind integrins, including αvβ3 and αvβ5, to regulate angiogenesis. To assess the role of EDIL3 derived from DMSCs of psoriasis (P-DMSCs) in angiogenesis, in vitro, EDIL3 of DMSCs from psoriasis was silenced by interfering EDIL3. Then the efficacy of silencing EDIL3 was tested by fluorescent flag, qRT-PCR and western blotting. And, in vitro, the relationship of EDIL3 in DMSCs with the angiogenesis of HUVECs were investigated through co-culture system. In vivo, EDIL3 recombinant protein was injected into IMQ cream-induced psoriasis-like skin lesions of mouse and EDIL3-associated tube formation were determined using Image J software. Our results showed the capacity of the adhesion, migration and tube formation of HUVECs in all psoriatic DMSCs groups were significantly higher compared with the control and si-EDIL3 groups (all P<0.05) in vitro. Moreover, under stimulated by EDIL3 recombinant protein, EDIL3-associated tube formation was dramatically elevated in vivo (P<0.01). In this study, EDIL3 could promote the adhesion, migration and tube formation of ECs and participant in the angiogenesis pathogenesis of psoriasis through affecting biological function on ECs both in vitro and in vivo. The results suggest a potential role of the critical pro-angiogenic factor EDIL3 in psoriasis therapy.
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http://dx.doi.org/10.1016/j.mvr.2020.104056DOI Listing
November 2020

sp. nov. and sp. nov., isolated from Tibetan antelope () and plateau pika (), respectively.

Int J Syst Evol Microbiol 2020 Aug;70(8):4683-4690

Department of Epidemiology, Shanxi Medical University School of Public Health, Taiyuan, Shanxi 030001, PR China.

Four novel strains (592, S592, MF47 and SMF47) were isolated from Tibetan antelopes () and plateau pikas (), respectively. The cells were aerobic, non-motile, Gram-stain- and catalase-positive, rod-shaped bacteria. The 16S rRNA gene sequences of the four strains showed highest similarities to DSM 10552 (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592 and MF47 represent two novel species. The four isolates produced acid from l-rhamnose, d-xylose and cellobiose, but were unable to reduce nitrate. The DNA G+C contents of strains 592 and MF47 were 70.3 and 69.8 mol%, respectively. The digital DNA-DNA hybridization value between strains 592 and MF47 was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species. The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus in the NCBI database, including NBRC 14897 and JCM 14732. The major fatty acids of the four strains were Cω9 and C 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant respiratory quinones were MK-9(H) and MK-8(H). The cell-wall peptidoglycan contained ll-diaminopimelic acid. Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, sp. nov. and sp. nov. The type strains are 592 (=CGMCC1.16526=DSM 106289) and MF47 (=CGMCC 1.17444=JCM 33790), respectively.
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http://dx.doi.org/10.1099/ijsem.0.004331DOI Listing
August 2020

n-BiSbTe: A Promising Alternative to Mainstream Thermoelectric Material n-BiTeSe near Room Temperature.

ACS Appl Mater Interfaces 2020 Jul 29;12(28):31619-31627. Epub 2020 Jun 29.

College of Materials Science and Engineering, Shenzhen Key Laboratory of Special Functional Materials, Guangdong Research Center for Interfacial Engineering of Functional Materials, Guangdong Provincial Key Laboratory of Deep Earth Sciences and Geothermal Energy Exploitation and Utilization, Institute of Deep Earth Sciences and Green Energy, Shenzhen University, Shenzhen 518060, P. R. China.

For decades, the VVI compounds, specifically p-type BiSbTe and n-type BiTeSe, have remained the cornerstone of commercial thermoelectric solid-state cooling and power generation near room temperature. However, a long-standing problem in VVI thermoelectrics is that n-type BiTeSe is inferior in performance to p-type BiSbTe near room temperature, restricting the device efficiency. In this work, we developed high-performance n-type BiSbTe, a composition long thought to only make good p-type thermoelectrics, to replace the mainstream n-type BiTeSe. The success arises from the synergy of the following mechanisms: (i) the donorlike effect, which produces excessive conduction electrons in BiTe, is compensated by the antisite defects regulated by Sb alloying; (ii) the conduction band degeneracy increases from 2 for BiTe and BiTeSe to 6 for BiSbTe, favoring high Seebeck coefficients; and (iii) the larger mass fluctuation yet smaller electronegativity difference and smaller atomic radius difference between Bi and Sb effectively suppresses the lattice thermal conductivity and retains decent carrier mobility. A state-of-the-art of 1.0 near room temperature was attained in hot deformed BiSbTe, which is higher than those for most known n-type thermoelectric materials, including commercial BiTeSe ingots and the popular MgSb. Technically, building both the n-leg and p-leg of a thermoelectric module using similar chemical compositions has key advantages in the mechanical strength and the durability of devices. These results attested to the promise of n-type BiSbTe as a replacement of the mainstream n-type BiTeSe near room temperature.
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http://dx.doi.org/10.1021/acsami.0c07566DOI Listing
July 2020

Cross-sectional study reveals thatHLA-C*07:02 is a potential biomarker of early onset/lesion severityof psoriasis.

Exp Dermatol 2020 Jun 7. Epub 2020 Jun 7.

Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, No. 5 Dong San Dao Xiang, Jiefang Road, Taiyuan, 030009, China.

Psoriasis is a common chronic autoimmune skin disease, with T cells playing a predominant role in its pathogenesis.Here, we aimed to investigate the relation of T cell repertoires (TCR) and major histocompatibility complex (MHC) in psoriatic patients to further understand mechanisms in disease pathogenesis.We conducted a cross-sectional study involving 9 pairs of monozygotic twins with inconsistent psoriasis and examined the TCR diversity and MHC haplotype ofthe individuals using multiple-PCR and high-throughput sequencing. Additionally, 665 psoriatic patients were applied to validate the relation of human leukocyte antigen (HLA)-class I allele HLA-C*07:02 and early onset or lesion severity of psoriasis.The immune diversity was lower in psoriatic patients compared with unaffected individuals within the twin pairs, although the difference was not significant.The clonotypes of TCR significantly decreased in psoriatic patients with high PASI score and early onset. HLA-C*07:02, a haplotype associated with psoriasis, was positively correlated with the diversity of the TCRV gene. Moreover, HLA-C*07:02 clustered in patients with high PASI and early onset. In the replication stage, we found that the PASI and onset age in psoriasis with HLA-C*07:02 were significantly different from those without HLA-C*07:02 and without HLA-C*06:02. Our observations indicate that HLA-C*07:02 is positively correlated with the diversity of TCRV gene in psoriasis, and maybe a potential biomarker of early onset/severe lesions of psoriasis.
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http://dx.doi.org/10.1111/exd.14127DOI Listing
June 2020

sp. nov., sp. nov., sp. nov. and sp. nov., isolated from faeces of Tibetan antelope in the Qinghai-Tibet plateau of China.

Int J Syst Evol Microbiol 2020 Jun;70(6):3763-3774

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing 102206, PR China.

Eight Gram-stain-positive, rod-shaped bacterial strains were isolated from faeces of Tibetan antelopes on the Tibet-Qinghai Plateau of China. Genomic sequence analysis showed that the strains belong to the genera (strains 299 and 340), (strains 2184, 2185, 2183 and 2189) and (strains 160 and 143), respectively, with a percentage of similarity for the 16S rRNA gene under the species threshold of 98.7 % except for strains 160 and 143 with CAU 1183 (98.8 %). The genome sizes (and genomic G+C contents) were 3.1 Mb (49.4 %), 2.5 Mb (64.9 %), 2.4 Mb (66.1 %) and 4.1 Mb (37.1 %) for the type strains 299, 2183, 2184 and 160, respectively. Two sets of the overall genome relatedness index values between our isolates and their corresponding closely related species were under species thresholds (95 % for average nucleotide identity, and 70 % for digital DNA-DNA hybridization). These results, together with deeper genotypic, genomic, phenotypic and biochemical analyses, indicate that these eight isolates should be classified as representing four novel species. Strain 299 (=CGMCC 1.16320=JCM 33611) is proposed as representing sp. nov.; strain 2184 (=CGMCC 1.16417=DSM 106203) is proposed as representing sp. nov.; strain 2183 (=CGMCC 1.16416=DSM 106264) is proposed as representing sp. nov.; and strain 160 (=CGMCC 1.16367=DSM 106186) is proposed as representing sp. nov.
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http://dx.doi.org/10.1099/ijsem.0.004232DOI Listing
June 2020

Different epigenome regulation and transcriptome expression of CD4 and CD8 T cells from monozygotic twins discordant for psoriasis.

Australas J Dermatol 2020 Nov 21;61(4):e388-e394. Epub 2020 May 21.

Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, China.

Objectives: Psoriasis is an immunodeficient skin disorder, and its exact pathogenesis is unclear. Monozygotic twins are presumed to be genetically identical, and their phenotypic differences may be due to transcriptional regulation or epigenome factors. To explain the inconsistency between twins, we have collected 3 pairs of monozygotic twins who are discordant for psoriasis.

Methods: Reduced representation of bisulfite sequencing and RNA sequencing was conducted using the peripheral blood of the twins to find the genes playing important roles in psoriasis pathogenesis.

Results: As a result, we found methylation diversity in four genes (MAST3, MTOR, PM20D1 and ZNF99), and we also found 9 differentially expressed genes (PPAN-P2RY11, PIGV, RPS18, TMEM121, KIF21A, KCNH2, WNT10B, PRX and CDH24) by RNA sequencing. According to the conjoint analysis of methylation and the mRNA results, PTPN6, CCL5, NFATC1 and PRF1 were found to be closely related to psoriasis. We then annotated the genes to explore the associations between these genes and psoriasis.

Conclusions: These findings provide a better understanding of psoriasis that can improve the diagnosis and treatment of the disease.
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http://dx.doi.org/10.1111/ajd.13325DOI Listing
November 2020

sp. nov.,isolated from faeces of Tibetan gazelle ().

Int J Syst Evol Microbiol 2020 Jun;70(6):3665-3672

Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing 100730, PR China.

Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136 and dk3543, were isolated from the faeces of Tibetan gazelle () in the Qinghai-Tibet Plateau of PR China. The cells were aerobic, oxidase-negative and catalase-positive. Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain-heart infusion (BHI) agar with 5 % sheep blood. The cells grew optimally at 28 °C, pH 7.5 and with 1 % (w/v) NaCl on BHI agar supplemented with 5 % sheep blood. Phylogenetic analysis of the 16S rRNA gene sequences revealed that their nearest phylogenetic relative was Ka25 (97.9 % similarity). The results of 16S rRNA gene sequence and phylogenetic/phylogenomic analyses illustrated that Ka25, XZ17, 78 and D287 were their nearest phylogenetic neighbours. The DNA G+C contents of strains dk3136 and dk3543 were 70.3 mol% and 70.4 mol%, respectively. Their genomes exhibit lower than threshold (95-96 %) average nucleotide identity to known species of the genus . ll-2,6-diaminopimelic acid was the diagnostic diamino acid and MK-8(H) was the predominant respiratory quinone. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The two strains had C9, -C and C8 as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars. On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136 and dk3543 represent a novel species in genus , for which the name sp. nov. is proposed. The type strain is dk3136 (=CGMCC 4.7570=JCM 33496=KCTC 49314).
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http://dx.doi.org/10.1099/ijsem.0.004218DOI Listing
June 2020

3D-bioprinted functional and biomimetic hydrogel scaffolds incorporated with nanosilicates to promote bone healing in rat calvarial defect model.

Mater Sci Eng C Mater Biol Appl 2020 Jul 30;112:110905. Epub 2020 Mar 30.

Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China. Electronic address:

Three-dimensional (3D) bioprinting is an extremely convenient biofabrication technique for creating biomimetic tissue-engineered bone constructs and has promising applications in regenerative medicine. However, existing bioinks have shown low mechanical strength, poor osteoinductive ability, and lacking a suitable microenvironment for laden cells. Nanosilicate (nSi) has shown to be a promising biomaterial, due to its unique properties such as excellent biocompatibility, degrade into nontoxic products, and with osteoinductive properties, which has been used in bone bioprinting. However, the long term bone healing effects and associating risks, if any, of using nSi in tissue engineering bone scaffolds in vivo are unclear and require a more thorough assessment prior to practical use. Hence, a functional and biomimetic nanocomposite bioink composed of rat bone marrow mesenchymal stem cells (rBMSCs), nSi, gelatin and alginate for the 3D bioprinting of tissue-engineered bone constructs is firstly demonstrated, mimicking the structure of extracellular matrix, to create a conducive microenvironment for encapsulated cells. It is shown that the addition of nSi significantly increases the printability and mechanical strength of fabricated human-scale tissue or organ structures (up to 15 mm height) and induces osteogenic differentiation of the encapsulated rBMSCs in the absence of in vitro osteoinductive factors. A systematic in vivo research of the biomimetic nanocomposite bioink scaffolds is further demonstrated in a rat critical-size (8 mm) bone defect-repair model. The in vivo results demonstrate that the 3D bioprinted nanocomposite scaffolds can significantly promote the bone healing of the rat calvarial defects compared to other scaffolds without nSi or cells, and show rarely side effects on the recipients. Given the above advantageous properties, the 3D bioprinted nanocomposite scaffolds can greatly accelerate the bone healing in critical bone defects, thus providing a clinical potential candidate for orthopedic applications.
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http://dx.doi.org/10.1016/j.msec.2020.110905DOI Listing
July 2020

Role of SPRED1 in keratinocyte proliferation in psoriasis.

J Dermatol 2020 Jul 12;47(7):735-742. Epub 2020 May 12.

Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, China.

Psoriasis is a recurrent inflammatory skin disease, affecting approximately 2% of the population. Previous studies have demonstrated that psoriatic dermal mesenchymal stem cells (DMSC) stimulated keratinocyte (KC) proliferation and that psoriasis exhibited missense SPRED1 mutations. To further investigate the molecular mechanism by which psoriatic DMSC stimulate KC proliferation, and the role of missense SPRED1 mutations in psoriasis, we assessed expression levels of miRNA, and both mRNA and protein of SPRED1 in normal human epidermal keratinocyte cells (NHEK) cocultured with either psoriatic or control DMSC. Expression levels of miRNA and mRNA were determined by RNA sequencing. Expression levels of spred1 protein were assessed using western blot analysis. Moreover, the variation in SPRED1 was also examined by whole-genome sequencing in 665 psoriatic patients, and verified by Sanger sequencing. Our results showed that coculture of NHEK with psoriatic DMSC induced 32 differentially expressed miRNA, in which expression levels of miR-1 increased approximately 16-fold over control DMSC-treated NHEK (P < 0.05). Likewise, expression levels of miR-21-3p increased over twofold (P < 0.05). Moreover, coculture of NHEK with psoriatic DMSC induced marked increase in expression levels of mRNA for MAPK3, CDC25B and CDC25C, while decreasing expression levels of SPRED1 mRNA and protein in comparison with control DMSC treatment (P < 0.05 for all between cocultured with control and psoriatic DMSC). Furthermore, psoriasis displayed non-synonymous mutation of SPRED1 enriched in exon 7: c.A881T:p.Y294F (chr15:38351210). These results suggest that dysregulation and mutations of SPRED1 may participate in the pathogenesis of psoriasis, including epidermal hyperproliferation.
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http://dx.doi.org/10.1111/1346-8138.15369DOI Listing
July 2020

sp. nov., isolated from leaves of on the Qinghai-Tibet Plateau.

Int J Syst Evol Microbiol 2020 May;70(5):3234-3240

Department of Epidemiology, Shanxi Medical University School of Public Health Taiyuan, Shanxi 030001, PR China.

Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144 and 4053, were isolated from leaves of on the Qinghai-Tibet Plateau, PR China. Cells were aerobic, catalase-positive and oxidase-negative. Colonies on Reasoner's 2A agar were light yellow, circular, shiny, smooth and convex after 2 days of incubation. The isolates grew optimally at 25 °C, pH 7.5 and with 0 % (w/v) NaCl. The results of polyphasic analyses indicated that strain S-1144 belonged to the genus and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were DSM 103718 (98.4 %), DSM 23986 (98.2%) and DSM 11054 (97.8 %). The genome of strain S-1144 showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains. The DNA G+C content of strain S-1144 was 73.5 mol%. MK-8(H) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The polar lipid profile of strain S-1144 consisted of diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, one unidentified glycolipid and one unidentified lipid. The major cellular fatty acids were iso-C, C8, C and C9. On the basis of obtained data, strain S-1144 represented a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S-1144 (=CGMCC 4.7568=JCM 33469).
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http://dx.doi.org/10.1099/ijsem.0.004161DOI Listing
May 2020

Expression and functional regulation of gap junction protein connexin 43 in dermal mesenchymal stem cells from psoriasis patients.

Acta Histochem 2020 May 14;122(4):151550. Epub 2020 Apr 14.

Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, No. 5 Dong San Dao Xiang, Jiefang Road, Taiyuan, 030009, Shanxi Province, China. Electronic address:

Background: Psoriasis is a chronic recurrent inflammatory disease. Mesenchymal stem cells (MSCs) can regulate the inflammatory microenvironment, thereby controlling the proliferation, differentiation, and migration of immune cells. Connexin 43(Cx43), a key gap junction protein, has been shown to form gap junctions for communication between neighboring cells.

Objective: We investigated the expression of Cx43 in dermal mesenchymal stem cells (DMSCs) derived from psoriasis patients and explored the relationship between the Cx43-mediated gap junction intercellular communication (GJIC) and DMSCs.

Methods: Human DMSCs were isolated and propagated in adherent culture. Quantitative real-time reverse transcription PCR and western blot and immunofluorescence were used to detect the expression and localization of Cx43 in DMSCs. Fluorescence redistribution after photobleaching was performed to assess adjacent DMSCs GJIC. CCK8 was used to detect the proliferation of DMSCs before and after gap junction blocker (18α-glycyrrhetinic acid; AGA) treatment. Cell energy metabolism was analyzed with an energy metabolism analyzer.

Results: Cx43 was located in the cytoplasm and cytomembrane, as well as partially in the nucleus of DMSCs. The expression of Cx43 in psoriasis DMSCs was higher than that in control samples and the gap junction function was enhanced. In addition, the glycolysis and mitochondrial respiration of psoriasis DMSCs were also enhanced. However, AGA inhibited the expression of Cx43, attenuated GJIC function, and inhibited the proliferation of DMSCs.

Conclusions: Our results indicated that the expression of Cx43 in DMSCs from psoriasis lesions is increased and that the inhibition of Cx43 leads to the inhibition of both GJIC and DMSCs proliferation.
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http://dx.doi.org/10.1016/j.acthis.2020.151550DOI Listing
May 2020

Psoriatic Dermal-derived Mesenchymal Stem Cells Reduce Keratinocyte Junctions, and Increase Glycolysis.

Acta Derm Venereol 2020 Apr 21;100(8):adv00122. Epub 2020 Apr 21.

Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, China.

Although it is known that psoriatic dermal-derived mesenchymal stem cells (DMSCs) dysregulate keratinocyte proliferation, the biological activity profile of keratinocytes influenced by psoriatic DMSCs remain unknown. In the present study, we assessed the impact of psoriatic DMSCs on keratinocyte proliferation, differentiation, and glucose metabolism in normal human epidermal keratinocytes co-cultured with or without psoriatic DMSCs. Co-culture of normal human epidermal keratinocytes with psoriatic DMSCs downregulated expression levels of proteins associated with cell junction assembly (alpha-actinin-1, catenin beta-1, poliovirus receptor-related protein 4 and procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2), while upregulating proteins associated with keratinocyte proliferation and differentiation (involucrin, isoform 2 of Histone-binding protein, isoform 3 of Telomeric repeat-binding factor 2 and keratin 13). Moreover, co-culture of normal human epidermal keratinocytes with psoriatic DMSCs stimulated keratinocyte proliferation and glycolysis, but reduced keratinocyte junctions. Taken together, these results demonstrate that psoriatic DMSCs increase keratinocyte proliferation and glycolysis, and reduce cell junctions, suggesting a pathogenic role of psoriatic DMSCs in epidermal hyperplasia, aberrant differentiation, and reduction in turnover time of keratinocytes in psoriasis.
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http://dx.doi.org/10.2340/00015555-3480DOI Listing
April 2020

Al-Si Alloy as a Diffusion Barrier for GeTe-Based Thermoelectric Legs with High Interfacial Reliability and Mechanical Strength.

ACS Appl Mater Interfaces 2020 Apr 8;12(16):18562-18569. Epub 2020 Apr 8.

College of Materials Sciences and Engineering, Shenzhen Key Laboratory of Special Functional Materials, Shenzhen Engineering Laboratory for Advanced Technology of Ceramics, and Guangdong Research Center for Interfacial Engineering of Functional, Shenzhen University, Shenzhen 518060, P. R. China.

To build high-performance thermoelectric (TE) devices for power generation, a suitable diffusion-barrier layer between the electrodes and the TE materials in a TE device is generally required for achieving good interfacial connection with high reliability, high mechanical strength but low electrical and thermal contact resistivities. GeTe-based materials have attracted great attention recently due to their high TE performance in the mid-temperature range, but studies on their TE devices are still limited. Here, we selected the AlSi alloy as a diffusion barrier for GeTe-based TE legs based on the matching test of the coefficient of thermal expansion. The good connection between AlSi and GeSbTeB is realized by the interfacial reaction, where the randomly distributed AlTe and Ge precipitates are formed at the interface of the joint. The as-prepared interfacial electrical contact resistivity can be as low as 20.7 μΩ·cm and only slightly increases to 26.1 μΩ·cm after 16 days of aging at 500 °C. Moreover, the shear strength of the joints can be as high as 26.6 MPa and unexpectedly increases to 41.7 MPa after 16 days of aging. The thickness of the reaction layer tends to be stabilized after 8 days of aging and nearly does not change after further aging to 16 days, which may be ascribed to the drag effect from Si and the secondary Ge phases. These results demonstrate the great potential of the Al-Si alloy as a diffusion barrier for GeTe-based TE devices with high performance.
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http://dx.doi.org/10.1021/acsami.0c02028DOI Listing
April 2020

sp. nov. and sp. nov. isolated from plateau pika () on the Qinghai-Tibet plateau of China.

Int J Syst Evol Microbiol 2020 Apr 20;70(4):2318-2324. Epub 2020 Mar 20.

Department of Epidemiology, Shanxi Medical University School of Public Health, Taiyuan, Shanxi 030001, PR China.

Four novel bacterial strains, designated Z294, Z311, Z443 and Z446, were isolated from the intestinal contents of plateau pika () on the Qinghai-Tibet Plateau of China. Cells were Gram-stain-positive, catalase-positive, oxidase-negative, aerobic, non-motile and short-rod shaped. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the four isolates belong to the genus , but clearly separate from the currently recognized species. Both type strains (Z294 and Z443) shared low 16S rRNA gene sequence similarity, digital DNA-DNA hybridization relatedness and average nucleotide identity values with NBRC 107612, JCM 19765, JCM 15130 and DSM 21501 and against each other. The genomic DNA G+C contents of strains Z294 and Z443 were 73.3 and 70 %, respectively. The major cellular fatty acids of strain Z294 were -C, -C A and C, in contrast to -C and -C A for strain Z443. Both type strains (Z294 and Z443) shared the following common features: glucose, rhamnose and ribose as cell-wall sugars; MK-8(H) as major menaquinone; alanine, glutamic acid and lysine as cell-wall amino acids; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified phosphoglycolipid as polar lipids. Comparing the phenotypic and phylogenetic features among the four strains and their related organisms, strains Z294 and Z443 represent two novel species within the genus , for which the names sp. nov. (type strain Z294=CGMCC 1.16428=DSM 106344) and sp. nov. (type strain Z443=CGMCC 1.16435=DSM 106174) are proposed.
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http://dx.doi.org/10.1099/ijsem.0.004042DOI Listing
April 2020

Zinc Silicate/Nano-Hydroxyapatite/Collagen Scaffolds Promote Angiogenesis and Bone Regeneration via the p38 MAPK Pathway in Activated Monocytes.

ACS Appl Mater Interfaces 2020 Apr 26;12(14):16058-16075. Epub 2020 Mar 26.

Department of Orthopedics, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, P. R. China.

Recent studies show that biomaterials are capable of regulating immune responses to induce a favorable osteogenic microenvironment and promote osteogenesis and angiogenesis. In this study, we investigated the effects of zinc silicate/nanohydroxyapatite/collagen (ZS/HA/Col) scaffolds on bone regeneration and angiogenesis and explored the related mechanism. We demonstrate that 10ZS/HA/Col scaffolds significantly enhanced bone regeneration and angiogenesis compared with HA/Col scaffolds. ZS/HA/Col scaffolds increased tartrate-resistant acid phosphatase (TRAP)-positive cells, nestin-positive bone marrow stromal cells (BMSCs) and CD31-positive neovessels, and expression of osteogenesis (2 and ) and angiogenesis-related ( and ) genes increased in nascent bone. ZS/HA/Col scaffolds with 10 wt % ZS activated the p38 signaling pathway in monocytes. The monocytes subsequently differentiated into TRAP cells and expressed higher levels of the cytokines SDF-1, TGF-β1, VEGF-α, and PDGF-BB, which recruited BMSCs and endothelial cells (ECs) to the defect areas. Blocking the p38 pathway in monocytes reduced TRAP differentiation and cytokine secretion and resulted in a decrease in BMSC and EC homing and angiogenesis. Overall, these findings demonstrate that 10ZS/HA/Col scaffolds modulate monocytes and, thereby, create a favorable osteogenic microenvironment that promotes BMSC migration and differentiation and vessel formation by activating the p38 signaling pathway.
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http://dx.doi.org/10.1021/acsami.0c00470DOI Listing
April 2020

sp. nov., isolated from plateau pika ().

Int J Syst Evol Microbiol 2020 Apr 25;70(4):2247-2253. Epub 2020 Feb 25.

Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing, PR China.

Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-A and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau. Optimal growth of these two strains was observed under aerobic conditions at pH 7.0 and 28 °C. The 16S rRNA gene sequences of the isolates had highest similarities of 98.5 and 98.4 % to , respectively. In the 16S rRNA gene and polygenetic trees, strains MF30-A and MF845 were clearly distinct from other species. The two strains could not produce acid from arbutin, d-fructose, D-sucrose, glycogen, salicin or starch. Production of β-glucosidase by these strains was negative. The major fatty acids of these strains were anteiso-C, anteiso-C and iso-C. Strain MF30-A contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones. The major polar lipids in strain MF30-A were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid. The G+C contents of the DNA of strains MF30-A and MF845 were 69.8 mol% and 69.7 mol%, respectively. The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus were far below the respective thresholds of 95 and 70 %, respectively. All genotypic and phenotypic data indicated that strains MF30-A and MF845 should be classified as novel members of the genus , for which the name sp. nov. is proposed. The type strain is MF30-A (=CGMCC 1.16469=DSM 106183).
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http://dx.doi.org/10.1099/ijsem.0.003987DOI Listing
April 2020

sp. nov., isolated from the intestinal contents of plateau pika () on the Qinghai-Tibet Plateau.

Int J Syst Evol Microbiol 2020 Apr 25;70(4):2233-2238. Epub 2020 Feb 25.

Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing, PR China.

Two Gram-staining-positive, catalase-positive, oxidase-negative, aerobic, non-motile, irregular rod-shaped bacterial strains (Z350 and Z527) were isolated from intestinal contents of plateau pika () from the Qinghai-Tibet Plateau, PR China. Results of phylogenetic analyses based on 16S rRNA gene sequences indicated that strain Z350 belongs to the genus (family ) but clearly differs from the currently recognized species DSM 101040 (98.4 % similarity) and DSM 27763 (97.4 %). Strain Z350 had a DNA G+C content of 70.7 mol% and shared 80.4 and 76.7 % average nucleotide identity values and 23.4 and 20.6 % DNA-DNA hybridization relatedness with DSM 101040 and DSM 27763, respectively. Further phylogenetic analyses based on 497 core genes indicated that our isolates were members of the genus but separated from all existing genera within the family . The major cellular fatty acids were C ω9 and 10-methyl C. The cell wall contained ll-diaminopimelic acid as the diamino acid, and rhamnose, ribose and glucose as whole cell-wall sugars. MK-9(H) was detected as the major menaquinone. Polar lipids present were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified phospholipid. Based on distinct differences in the genotypic and phenotypic data from the two species, a novel species, sp. nov., is proposed. The type strain is Z350 (=CGMCC 4.7464=DSM 106288).
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http://dx.doi.org/10.1099/ijsem.0.003979DOI Listing
April 2020

sp. nov., isolated from faeces of Tibetan antelope () on the Qinghai-Tibet Plateau.

Int J Syst Evol Microbiol 2020 Apr 7;70(4):2204-2210. Epub 2020 Feb 7.

Research Units of Discovery of Unknown Bacteria and Function, Chinese Academy of Medical Sciences, Beijing, PR China.

Two Gram-stain-positive, catalase-positive and oxidase-negative, aerobic, non-motile, cellobiose-utilizing, short-rod-shaped strains (Z28 and Z29) were isolated from faeces of Tibetan antelope () collected on the Qinghai-Tibet Plateau. Strain Z28 shared 98.1, 98.0, 97.8 and 97.4 % 16S rRNA gene similarity, 24.1, 22.8, 23.2 and 26.3 % digital DNA-DNA hybridization relatedness and 80.8, 80.0, 80.7 and 80.9 % average nucleotide identity values with DSM 24482, DSM 20109, DSM 14785 and JCM 14899, respectively. Results from further phylogenetic analyses based on the 16S rRNA gene and 148 core genes indicated that strains Z28 and Z29 were closest to DSM 24482 and DSM 20109, but clearly separated from the currently recognized species of the genus . The genomic DNA G+C content of strain Z28 was 75.3 mol%. The major cellular fatty acids were -C, -C A, C and -C. Ribose and mannose were detected as the whole-cell sugars. The major respiratory quinone was MK-9(H) and ornithine was the diamino acid of the cell wall. The polar lipids present in strain Z28 were phosphatidylethanolamine, five phospholipids, two aminophospholipids, aminolipid and three unidentified lipids. Comparison of phenotypic and phylogenetic features between the two strains and the related organisms revealed that Z28 and Z29 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Z28 (=CGMCC 1.16477=DSM 106200).
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http://dx.doi.org/10.1099/ijsem.0.003939DOI Listing
April 2020