Publications by authors named "Junping Wang"

206 Publications

Circ_0084615 is an oncogenic circular RNA in colorectal cancer and promotes DNMT3A expression via repressing miR-599.

Pathol Res Pract 2021 May 27;224:153494. Epub 2021 May 27.

Department of Gastroenterology, Peking University Shenzhen Hospital, No. 1120 Lianhua Road, Futian District, Shenzhen, 518036, Guangdong, China. Electronic address:

Background: Circular RNAs (circRNAs) are implicated in modulating cancer progression, exerting a pro- or anti-cancer effect. This work is aimed to probe the biological function of circ_0084615 in colorectal cancer (CRC) and its underlying mechanism.

Methods: Circ_0084615 was selected from two circRNA microarray datasets (GSE138589 and GSE142837). Circ_0084615, microRNA (miR)-599 and DNA methyltransferases 3A (DNMT3A) mRNA expression in CRC tissues and cell lines were examined by qRT-PCR. The relationship between circ_0084615 expression level and clinical features were analyzed with chi-square test. Circ_0084615 knockdown model was constructed by siRNA in two CRC cell lines. The biological functions of circ_0084615 in CRC cells were evaluated by CCK-8 and Transwell experiments. The effect of circ_0084615 on CRC cell metastasis in vivo was examined with lung metastasis model of nude mice. Dual luciferase reporter gene assay was used to determine whether circ_0084615 and miR-599, and miR-599 and DNMT3A interacted with each other. Western blot was employed to examine the regulatory effects of circ_0084615 and miR-599 on DNMT3A protein expression in CRC cells.

Results: Circ_0084615 was up-regulated in CRC and was correlated with poor overall survival rate and advanced clinical stage of CRC patients. Functional assays validated that depletion of circ_0084615 impeded CRC cell proliferation, migration and invasion. Circ_0084615 acted as a molecular sponge for miR-599 to repress its expression. DNMT3A was a downstream target of miR-599. Functional compensation experiments showed that miR-599 inhibitors partially counteracted the the biological effects of silencing circ_0084615 on CRC cells.

Conclusions: Circ_0084615 is a tumor-promoting circRNA in CRC that functions as a competing endogenous RNA to regulate DNMT3A expression via sponging miR-599. Our research provides a potential therapeutic target for CRC patients.
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http://dx.doi.org/10.1016/j.prp.2021.153494DOI Listing
May 2021

MiRNA-29c-3p Promotes Intestinal Inflammation via Targeting Leukemia Inhibitory Factor in Ulcerative Colitis.

J Inflamm Res 2021 18;14:2031-2043. Epub 2021 May 18.

Department of Gastroenterology, Shanxi Provincial People's Hospital, The Affiliated People's Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030012, People's Republic of China.

Background: Dysregulation of micro-RNAs (miRNAs) is profoundly linked to inflammatory bowel diseases (IBD), but little is known about the specific biological functions of miRNAs in IBD. This study sought to elucidate the effect and the underlying target of miR-29c-3p in ulcerative colitis (UC).

Methods: The levels of miR-29c-3p and leukemia inhibitory factor (LIF) were measured in inflamed lesions of UC patients and dextran sulfate sodium (DSS)-induced colitis mice by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. MiR-29c-3p was predicted to target LIF by bioinformatics software, which was verified via luciferase reporter assay and transfection of miR-29c-3p mimics or inhibitor. The role of miR-29c-3p/LIF axis in intestinal inflammation was explored in experimental colitis mice and Caco-2 cells.

Results: MiR-29c-3p was markedly downregulated while LIF was upregulated in colon tissues of UC patients and DSS-challenged colitis mice as well as in primary intestinal epithelial cells (IECs) and LPS-treated Caco-2 cells. MiR-29c-3p inhibited LIF expression at the transcriptional level via binding to LIF 3'-untranslated region (UTR) in Caco-2 cells. Targeting miR-29c-3p/LIF axis regulated inflammatory cytokines production, cell proliferation and apoptosis. Overexpression of miR-29c-3p aggravated mice experimental colitis via suppressing LIF.

Conclusion: Our findings demonstrate that the upregulation of miR-29c-3p promotes gut inflammation and the expression of pro-inflammatory mediators via suppressing LIF, thereby modulating the pathogenesis of UC.
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http://dx.doi.org/10.2147/JIR.S302832DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140949PMC
May 2021

Polydatin has anti-inflammatory and antioxidant effects in LPS-induced macrophages and improves DSS-induced mice colitis.

Immun Inflamm Dis 2021 May 19. Epub 2021 May 19.

Central Laboratory, Shanxi Provincial People's Hospital, Affiliate of Shanxi Medical University, Taiyuan, Shanxi, China.

Polydatin (PD), a monocrystalline compound isolated from the root and rhizome of Polygonum cuspidatum, is widely used in inhibiting the inflammatory response and oxidative stress. PD has an anti-inflammatory effect on colitis mice; however, information regulating the mechanism by which maintains the intestinal epithelium barrier is currently scarce. Here, we assessed the anti-inflammatory and antioxidant of PD in lipopolysaccharide (LPS)-induced macrophages in vitro, and explored its effects on inhibiting intestinal inflammation and maintaining the intestinal epithelium barrier in dextran sodium sulfate (DSS)-induced colitis mice. Results showed that PD reduced the level of proinflammatory cytokines and enzymes, including tumor necrosis factor-α, interleukin-4 (IL-4), IL-6, cyclooxygenase-2, and inducible nitric oxide synthase, in LPS-induced macrophages, and improved the expression level of IL-10. PD maintained the expression of tight junction proteins in medium (LPS-induced macrophages medium)-induced MCEC cells. Additionally, PD inhibited the phosphorylation of nuclear factor-κB (NF-κB), p65, extracellular signal-regulated kinase-1/2, c-Jun N-terminal kinase, and p38 signaling pathways in LPS-induced macrophages and facilitated the phosphorylation of AKT and the nuclear translocation of Nrf2, improving the expression of HO-1 and NQO1. Furthermore, PD ameliorated the intestinal inflammatory response and improved the dysfunction of the colon epithelium barrier in DSS-induced colitis mice. Taken together, our results indicated that PD inhibited inflammation and oxidative stress, maintained the intestinal epithelium barrier, and the protective role of PD was associated with the NF-κB p65, itogen-activated protein kinases, and AKT/Nrf2/HO-1/NQO1 signaling pathway.
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http://dx.doi.org/10.1002/iid3.455DOI Listing
May 2021

The effects of S-nitrosylation-induced PPARγ/SFRP5 pathway inhibition on the conversion of non-alcoholic fatty liver to non-alcoholic steatohepatitis.

Ann Transl Med 2021 Apr;9(8):684

Gastroenterology Department, Shanxi Provincial People's Hospital, Taiyuan, China.

Background: Peroxisome proliferators-activated receptors γ (PPARγ) and secreted frizzled related protein 5 (SFRP5) are abnormally expressed in liver cells. But their role in the transformation of non-alcoholic fatty liver (NAFL) to non-alcoholic steatohepatitis (NASH) remains to be studied. We aimed to explore the role of S-nitrosylation (SNO) in the conversion of NAFL to NASH via the peroxisome PPARγ/SFRP5 pathway.

Methods: A normal diet and methionine-choline deficient diet were used to construct the NAFL and NASH mouse models, respectively. The differences between the SNO of PPARγ in both models were measured by irreversible biotinylation. Quantitative reverse transcription PCR (qRT-PCR) and Western blotting were used to detect the effect of SNO on the expression of PPARγ messageRNA (mRNA) and protein in L02 hepatocytes. Nubiscan software, luciferase reporter gene, and chromatin immunoprecipitation assay (CHIP) were used to verify the targeting relationship between PPAR and SFRP5. The expression of tumor necrosis factor α (TNFα), interleukin-1β (IL-1β), and interleukin-6 (IL-6), which are indicators for the activation of Kupffer cells, were determined by enzyme linked immunosorbent assay (ELISA) after co-cultivation of L02 hepatocytes and Kupffer macrophages, as well as the exogenous regulation of SNO, PPARγ, and SFRP5 in hepatic L02 cells.

Results: The NAFL and NASH mouse models were successfully constructed, and the level of PPARγ SNO in the NAFL model was significantly lower than the NASH model (P<0.05). The level of PPARγ was significantly downregulated after increasing the SNO of L02 cells, respectively (P<0.05). Nubiscan software and CHIP confirmed that PPARγ could bind to the promoter region of SFRP5 (P<0.05). Overexpression of PPARγ and SFRP5 could significantly downregulate the expression of TNFα, IL-1β, and IL-6 (P<0.05) correspondingly, while increasing the SNO level of L02 cells could restore the expression levels of TNFα, IL-1β, and IL-6.

Conclusions: SNO promoted the activation of macrophage Kupffer cells by inhibiting the PPARγ/SFRP5 pathway in L02 hepatocytes, thereby promoting the conversion of NAFL into NASH.
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http://dx.doi.org/10.21037/atm-21-1070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106108PMC
April 2021

Application of Visual Gene Clip-Based Tailored Therapy for the Eradication of .

Biomed Res Int 2021 10;2021:6150628. Epub 2021 Apr 10.

Department of Gastroenterology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, 518036, China.

Background: eradication with therapies employing a proton pump inhibitor (PPI) and antimicrobial agents is mainly achieved via bacterial susceptibility to antimicrobial agents and the magnitude of acid secretion inhibition. However, annual eradication rates have greatly declined in Mainland China, and therefore, tailored eradication regimens that inhibit acid secretion and employ optimal antimicrobial agents determined based on gene clip testing may improve eradication rates. This study was aimed at evaluating the efficacy of tailored eradication therapy guided by visual gene clip testing for antibiotic resistance and PPI metabolism genotypes.

Methods: This prospective study included 244 patients (141 men and 103 women aged 20-79 years) receiving initial treatment for infection. Visual gene clip testing using gastric mucosal specimens was performed to detect antibiotic resistance to clarithromycin conferred by the A2142G and A2143G point mutations of the 23S rRNA gene and to levofloxacin conferred by the Asn87 and Asp91 point mutations of the gene. Patients received a 14-day bismuth quadruple therapy regimen guided by testing for antibiotic resistance and CYP2C19 polymorphisms, and primary eradication was assessed at least 4 weeks after therapy.

Results: strains were successfully isolated from the gastric mucosa tissues of 244 patients. Antibiotic resistant isolates were identified in 63 patients, with clarithromycin resistance observed in 50 patients, levofloxacin resistance in 7 patients, and dual resistance in 6 patients. The PPI metabolic genotype of CYP2C19 was detected in 242 of 244 cases, and 97 cases were categorized as extensive metabolizers, 141 as intermediate metabolizers, and 4 as poor metabolizers. Among the 242 patients who received tailored therapy, the eradication rate was 90.9% (95% confidence interval 87.3%~94.6%) in the intention-to-treat analysis and 96.9% (95% confidence interval 94.7%~99.2%) in the per protocol analysis.

Conclusions: Tailored therapy for infection guided by determination of antibiotic resistance and CYP2C19 polymorphism using visual gene chip technology may provide high clinical effectiveness as initial eradication therapy.
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http://dx.doi.org/10.1155/2021/6150628DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055396PMC
May 2021

Development of non-enzymatic and photothermal immuno-sensing assay for detecting the enrofloxacin in animal derived food by utilizing black phosphorus-platinum two-dimensional nanomaterials.

Food Chem 2021 Apr 21;357:129766. Epub 2021 Apr 21.

State Key Laboratory for Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address:

The two-dimensional black phosphorus nanosheets (BPNSs) provide strong support for the construction of nanozymes with high catalytic performance due to the sheet structure and high electronic activity. A peroxidase-like BP-Pt nanocomposites was successfully synthesized using the instability of BPNS, a non-enzymatic immunosensing assay (NISA) was established with BP-Pt as immunosensing probe. Take the antibiotic enrofloxacin (ENR) as the target, NISA realized the highly sensitive ENR detection with detection limit (IC) of 0.005 μg/L. In addition, based on the good photothermal performance of oxTMB at 808 nm, a photothermal immunosensing assay (PT-NISA) was established, and ENR detection results was similar to NISA were obtained. In the analysis of the samples, the same detection results as the commercially available enzyme-linked immunoassay kit were obtained. These NISA and PT-NISA provide a more rapid and promising strategy for detecting food contaminants, and was expected to be used to detect other highly sensitive biological macromolecules.
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http://dx.doi.org/10.1016/j.foodchem.2021.129766DOI Listing
April 2021

Human Cathelicidin Inhibits SARS-CoV-2 Infection: Killing Two Birds with One Stone.

ACS Infect Dis 2021 Jun 14;7(6):1545-1554. Epub 2021 Apr 14.

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury of PLA, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.

SARS-CoV-2 infection begins with the association of its spike 1 (S1) protein with host angiotensin-converting enzyme-2 (ACE2). Targeting the interaction between S1 and ACE2 is a practical strategy against SARS-CoV-2 infection. Herein, we show encouraging results indicating that human cathelicidin LL37 can simultaneously block viral S1 and cloak ACE2. LL37 binds to the receptor-binding domain (RBD) of S1 with high affinity (11.2 nM) and decreases subsequent recruitment of ACE2. Owing to the RBD blockade, LL37 inhibits SARS-CoV-2 S pseudovirion infection, with a half-maximal inhibitory concentration of 4.74 μg/mL. Interestingly, LL37 also binds to ACE2 with an affinity of 25.5 nM and cloaks the ligand-binding domain (LBD), thereby decreasing S1 adherence and protecting cells against pseudovirion infection . Intranasal administration of LL37 to C57 mice infected with adenovirus expressing human ACE2 either before or after pseudovirion invasion decreased lung infection. The study identified a versatile antimicrobial peptide in humans as an inhibitor of SARS-CoV-2 attachment using dual mechanisms, thus providing a potential candidate for coronavirus disease 2019 (COVID-19) prevention and treatment.
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http://dx.doi.org/10.1021/acsinfecdis.1c00096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056948PMC
June 2021

Membrane Nanoparticles Derived from ACE2-Rich Cells Block SARS-CoV-2 Infection.

ACS Nano 2021 04 18;15(4):6340-6351. Epub 2021 Mar 18.

Department of Nephrology, The Key Laboratory for The Prevention and Treatment of Chronic Kidney Disease of Chongqing, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.

The ongoing COVID-19 pandemic worldwide necessitates the development of therapeutics against SARS-CoV-2. ACE2 is the main receptor of SARS-CoV-2 S1 and mediates viral entry into host cells. Herein, membrane nanoparticles (NPs) prepared from ACE2-rich cells were discovered to have potent capacity to block SARS-CoV-2 infection. The membranes of human embryonic kidney-239T cells highly expressing ACE2 were applied to prepare NPs using an extrusion method. The nanomaterials, termed ACE2-NPs, contained 265.1 ng mg ACE2 on the surface and acted as baits to trap S1 in a dose-dependent manner, resulting in reduced recruitment of the viral ligand to HK-2 human renal tubular epithelial cells. Aside from affecting receptor recongnition, S1 translocated to the cytoplasm and induced apoptosis by reducing optic atrophy 1 expression and increasing cytochrome c release, which was also inhibited by ACE2-NPs. Further investigations revealed that ACE2-NPs efficiently suppressed SARS-CoV-2 S pseudovirions entry into host cells and blocked viral infection and . This study characterizes easy-to-produce memrbane nanoantagonists of SARS-CoV-2 that enrich the existing antiviral arsenal and provide possibilities for COVID-19 treatment.
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http://dx.doi.org/10.1021/acsnano.0c06836DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8009101PMC
April 2021

Neutrophils-derived Spink7 as one safeguard against experimental murine colitis.

Biochim Biophys Acta Mol Basis Dis 2021 Jun 13;1867(6):166125. Epub 2021 Mar 13.

Institute of Combined Injury, State Key Laboratory of Trauma, Burn and Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China. Electronic address:

The uncontrolled abnormal intestinal immune responses play important role in eliciting inflammatory bowel disease (IBD), yet the molecular events regulating intestinal inflammation during IBD remain poorly understood. Here, we describe an endogenous, homeostatic pattern that controls inflammatory responses in experimental murine colitis. We show that Spink7 (serine peptidase inhibitor, kazal type 7), the ortholog of human SPINK7, is significantly upregulated in dextran sodium sulfate (DSS)-induced murine colitis model. Spink7-deficient mice showed highly susceptible to experimental colitis characterized by enhanced weight loss, shorter colon length, higher disease activity index and increased colonic tissue destruction. Bone marrow reconstitution experiments demonstrated that expression of Spink7 in the immune compartment makes main contribution to its protective role in colitis. What's more, neutrophils are the primary sources of Spink7 in experimental murine colitis. Loss of Spink7 leads to augmented productions of multiple chemokines and cytokines in colitis. In summary, this study identifies neutrophils-derived endogenous Spink7-mediated control of chemokines/cytokines production as a molecular mechanism contributing to inflammation resolution during colitis.
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http://dx.doi.org/10.1016/j.bbadis.2021.166125DOI Listing
June 2021

Mitochondrial Damage-Induced Innate Immune Activation in Vascular Smooth Muscle Cells Promotes Chronic Kidney Disease-Associated Plaque Vulnerability.

Adv Sci (Weinh) 2021 Mar 6;8(5):2002738. Epub 2021 Jan 6.

Department of Nephrology the Key Laboratory for the Prevention and Treatment of Chronic Kidney Disease of Chongqing Kidney Center of PLA Xinqiao Hospital Army Medical University (Third Military Medical University) Chongqing 400037 China.

Chronic kidney disease (CKD) is associated with accelerated atherosclerosis progression and high incidence of cardiovascular events, hinting that atherosclerotic plaques in CKD may be vulnerable. However, its cause and mechanism remain obscure. Here, it is shown that apolipoprotein E-deficient (ApoE) mouse with CKD (CKD/ApoE mouse) is a useful model for investigating the pathogenesis of plaque vulnerability, and premature senescence and phenotypic switching of vascular smooth muscle cells (VSMCs) contributes to CKD-associated plaque vulnerability. Subsequently, VSMC phenotypes in patients with CKD and CKD/ApoE mice are comprehensively investigated. Using multi-omics analysis and targeted and VSMC-specific gene knockout mice, VSMCs are identified as both type-I-interferon (IFN-I)-responsive and IFN-I-productive cells. Mechanistically, mitochondrial damage resulting from CKD-induced oxidative stress primes the cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway to trigger IFN-I response in VSMCs. Enhanced IFN-I response then induces VSMC premature senescence and phenotypic switching in an autocrine/paracrine manner, resulting in the loss of fibrous cap VSMCs and fibrous cap thinning. Conversely, blocking IFN-I response remarkably attenuates CKD-associated plaque vulnerability. These findings reveal that IFN-I response in VSMCs through immune sensing of mitochondrial damage is essential for the pathogenesis of CKD-associated plaque vulnerability. Mitigating IFN-I response may hold promise for the treatment of CKD-associated cardiovascular diseases.
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http://dx.doi.org/10.1002/advs.202002738DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927614PMC
March 2021

Correlation between cortical gene expression and resting-state functional network centrality in healthy young adults.

Hum Brain Mapp 2021 May 11;42(7):2236-2249. Epub 2021 Feb 11.

Department of Radiology and Tianjin Key Laboratory of Functional Imaging, Tianjin Medical University General Hospital, Tianjin, China.

Resting-state functional connectivity in the human brain is heritable, and previous studies have investigated the genetic basis underlying functional connectivity. However, at present, the molecular mechanisms associated with functional network centrality are still largely unknown. In this study, functional networks were constructed, and the graph-theory method was employed to calculate network centrality in 100 healthy young adults from the Human Connectome Project. Specifically, functional connectivity strength (FCS), also known as the "degree centrality" of weighted networks, is calculated to measure functional network centrality. A multivariate technique of partial least squares regression (PLSR) was then conducted to identify genes whose spatial expression profiles best predicted the FCS distribution. We found that FCS spatial distribution was significantly positively correlated with the expression of genes defined by the first PLSR component. The FCS-related genes we identified were significantly enriched for ion channels, axon guidance, and synaptic transmission. Moreover, FCS-related genes were preferentially expressed in cortical neurons and young adulthood and were enriched in numerous neurodegenerative and neuropsychiatric disorders. Furthermore, a series of validation and robustness analyses demonstrated the reliability of the results. Overall, our results suggest that the spatial distribution of FCS is modulated by the expression of a set of genes associated with ion channels, axon guidance, and synaptic transmission.
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http://dx.doi.org/10.1002/hbm.25362DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8046072PMC
May 2021

[Clinical and Imaging Features of Acute Cerebral Infarction 
in Non-small Cell Lung Cancer Patients with Trousseau Syndrome].

Zhongguo Fei Ai Za Zhi 2021 Jan;24(1):13-18

Department of Radiology, Tianjin Key Laboratory of Functional Imaging, Tianjin Medical University General Hospital, 
Tianjin 300052, China.

Background: Acute cerebral infarction is a form of Trousseau syndrome (TS), but is relatively rare and often overlooked by clinicians. The aim of this study was to investigate the clinical, laboratory and imaging features of acute cerebral infarction in non-small cell lung cancer (NSCLC) patients with TS.

Methods: Clinical data, laboratory examination and imaging data of 25 NSCLC patients with TS presented with acute cerebral infarction were collected retrospectively for analysis.

Results: Of the 25 patients, 18 males and 7 females, aged 39-78 years old, including 22 cases of adenocarcinoma, 2 cases of squamous cell carcinoma, and 1 case of large cell carcinoma; all patients had clinical symptoms and signs of acute cerebral infarction; plasma D-dimer was significantly increased, and prothrombin time and activated partial thromboplastin time were shortened to varying degrees; all patients showed acute multiple cerebral infarction foci involving multiple intracranial arterial blood supply areas on plain head magnetic resonance imaging (MRI) [diffusion-weighted imaging (DWI) sequence], the blood supply vessel lumen corresponding to the infarction foci did not show moderate to severe stenosis on the head MR angiography (MRA).

Conclusions: NSCLC with multiple acute cerebral infarctions is a rare manifestation of TS, which is characterized by multiple acute cerebral infarctions involving multiple arterial blood supply areas with significant hypercoagulability. Improving the early understanding of this disease can provide some help for clinical diagnosis and treatment.
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http://dx.doi.org/10.3779/j.issn.1009-3419.2021.102.01DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849036PMC
January 2021

Inflammasomes and the Maintenance of Hematopoietic Homeostasis: New Perspectives and Opportunities.

Molecules 2021 Jan 9;26(2). Epub 2021 Jan 9.

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China.

Hematopoietic stem cells (HSCs) regularly produce various blood cells throughout life via their self-renewal, proliferation, and differentiation abilities. Most HSCs remain quiescent in the bone marrow (BM) and respond in a timely manner to either physiological or pathological cues, but the underlying mechanisms remain to be further elucidated. In the past few years, accumulating evidence has highlighted an intermediate role of inflammasome activation in hematopoietic maintenance, post-hematopoietic transplantation complications, and senescence. As a cytosolic protein complex, the inflammasome participates in immune responses by generating a caspase cascade and inducing cytokine secretion. This process is generally triggered by signals from purinergic receptors that integrate extracellular stimuli such as the metabolic factor ATP via P2 receptors. Furthermore, targeted modulation/inhibition of specific inflammasomes may help to maintain/restore adequate hematopoietic homeostasis. In this review, we will first summarize the possible relationships between inflammasome activation and homeostasis based on certain interesting phenomena. The cellular and molecular mechanism by which purinergic receptors integrate extracellular cues to activate inflammasomes inside HSCs will then be described. We will also discuss the therapeutic potential of targeting inflammasomes and their components in some diseases through pharmacological or genetic strategies.
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http://dx.doi.org/10.3390/molecules26020309DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827629PMC
January 2021

The cGAS-STING Pathway in Hematopoiesis and Its Physiopathological Significance.

Front Immunol 2020 30;11:573915. Epub 2020 Nov 30.

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing, China.

Cytosolic DNA sensing is a fundamental mechanism by which organisms handle various stresses, including infection and genotoxicity. The hematopoietic system is sensitive to stresses, and hematopoietic changes are often rapid and the first response to stresses. Based on the transcriptome database, cytosolic DNA sensing pathways are widely expressed in the hematopoietic system, and components of these pathways may be expressed at even higher levels in hematopoietic stem and progenitor cells (HSPCs) than in their certain progeny immune cells. Recent studies have described a previously unrecognized role for cytosolic DNA sensing pathways in the regulation of hematopoiesis under both homeostatic and stress conditions. In particular, the recently discovered cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway is a critical modulator of hematopoiesis. Perturbation of the cGAS-STING pathway in HSPCs may be involved in the pathogenesis of hematopoietic disorders, autoimmune diseases, and inflammation-related diseases and may be candidate therapeutic targets. In this review, we focus on the recent findings of the cGAS-STING pathway in the regulation of hematopoiesis, and its physiopathological significance including its implications in diseases and therapeutic potential.
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http://dx.doi.org/10.3389/fimmu.2020.573915DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734179PMC
November 2020

Quartz crystal microbalance sensor based on 11-mercaptoundecanoic acid self-assembly and amidated nano-titanium film for selective and ultrafast detection of phosphoproteins in food.

Food Chem 2021 May 18;344:128656. Epub 2020 Nov 18.

Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science & Technology, 29 The Thirteenth Road, Tianjin Economy and Technology Development Area, Tianjin 300457, PR China. Electronic address:

A novel quartz crystal microbalance (QCM) sensor for trace-phosphoprotein ultrafast detection was constructed based on the bridge interactions between the NH-TiO sites enriched on Au-electrode and phosphate groups. Herein, 11-mercaptoundecanoic acid (MUA) modified by Au-S bond acted as carrier for immobilizing NH-TiO. Functionalized NH-TiO to absorb phosphoproteins. Under the optimal conditions, the proposed sensor showed a linear frequency shift to the concentration of α-casein ranging from 1.0 × 10 to 1.0 mg mL with a low detection limit of 5.3 × 10 mg mL (S/N = 3), and the limit of quantitation was 0.001 mg mL. Compared with traditional Ti-IMAC/MOAC-system, the analysis process of NH-TiO/MUA/AuE-QCM sensor was simpler and faster which could complete within 5 min. Additionally, the constructed biosensor was successfully used for the non-fat milk and chicken egg white. This proposed sensor presents a great prospective strategy for the evaluation of the nutrition in different foods.
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http://dx.doi.org/10.1016/j.foodchem.2020.128656DOI Listing
May 2021

Determination of Trace Phosphoprotein in Food Based on Fluorescent Probe-Triggered Target-Induced Quench by Electrochemiluminescence.

J Agric Food Chem 2020 Nov 27;68(45):12738-12748. Epub 2020 Oct 27.

Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, 29 The Thirteenth Road, Tianjin Economy and Technology Development Area, Tianjin 300457, P.R. China.

Evaluation of the nutrition and determination of phosphoproteins is of great importance in different foods as aberrant phosphorylation changes many biological processes and can relate to health conditions. In this study, an ultrafast (5 min) and sensitive electrochemiluminescence (ECL) sensor was innovatively fabricated for the determination of phosphoproteins in foods on the basis of fluorescent probe NH-TiO/upconversion nanomaterials (UCNPs). Impressively, the ECL intensity of NH-TiO/UCNPs-rGO/GCE was remarkably enhanced by 29 times. Furthermore, the photoactive NH-TiO layer provided not only specific selectivity but also a large surface area as well as an unprecedented photocatalytic activity for the NH-TiO/UCNPs-rGO/GCE ECL sensor (TIECLS), which could serve as an identification element for trace phosphoproteins. Under optimal conditions, the TIECLS achieved a relatively low detection limit of 9.2 × 10 mg/mL (/ = 3). Practical application of this TIECLS was carried out in different food samples with satisfying results, which were validated by laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS).
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http://dx.doi.org/10.1021/acs.jafc.0c05308DOI Listing
November 2020

Dietary quercetin attenuates depressive-like behaviors by inhibiting astrocyte reactivation in response to stress.

Biochem Biophys Res Commun 2020 12 12;533(4):1338-1346. Epub 2020 Oct 12.

Department of Pharmacy, Cancer Hospital, Chinese Academy of Sciences, Hefei, Anhui, 230001, China. Electronic address:

The mechanisms underlying the antidepressant activity of quercetin are unknown. We investigated the effect of a quercetin-enriched diet (2 g/kg and 0.5 g/kg doses) on chronic social defeat stress (CSDS)-induced depressive-like behaviors in mice. The 2 g/kg quercetin-enriched diet attenuated depressive-like behaviors when introduced before CSDS (long-term). The long-term 0.5 g/kg quercetin-enriched diet showed a trend toward behavioral improvement. The frequencies of spontaneous excitatory postsynaptic currents (sEPSCs) and spontaneous inhibitory postsynaptic currents (sIPSCs) in the mPFC and hippocampus were significantly higher in mice fed the long-term 2 g/kg quercetin-enriched diet compared with the normal diet; no difference was found in the amygdala. Quercetin-enriched diets administered concurrently and after stress induction failed to trigger these effects. A1-specific astrocyte reactivity was markedly suppressed in the microglia and astrocytes isolated from the mPFC and hippocampus of mice fed the long-term quercetin-enriched diet, but not in those who received quercetin supplementation concurrently or after CSDS. To confirm the role of astrocytes in the neuroprotective effect of quercetin, we activated astrocytes by injecting a chemogenic AAV stimulus into the mPFC and hippocampus and found that astrocyte activation during administration of the long-term quercetin-enriched diet significantly deceased the frequency of sEPSCs and sIPSCs in the mPFC and hippocampus and further attenuated quercetin-induced behavioral improvements. These findings highlight the key role of astrocyte reactivation in the regulation of quercetin neuroprotective activity and suggest that a diet high in quercetin, whether as a fruit- and vegetable-rich diet or food additive may help cope with stress.
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http://dx.doi.org/10.1016/j.bbrc.2020.10.016DOI Listing
December 2020

Rapid Screening of Nitrogen Use Efficiency in Perennial Ryegrass ( L.) Using Automated Image-Based Phenotyping.

Front Plant Sci 2020 27;11:565361. Epub 2020 Aug 27.

Agriculture Victoria Research, Hamilton, VIC, Australia.

Perennial ryegrass ( L.) is a dominant species in temperate Australian pastures. Currently, nitrogenous fertilizers are used to support herbage production for pasture and fodder. Increasing the nitrogen use efficiency (NUE) of pasture grasses could decrease the amount of fertilizer application and reduce nitrogen (N) leaching into the environment. NUE, defined as units of dry matter production per unit of supplied nitrogen, is a complex trait in which genomic selection may provide a promising strategy in breeding. Our objective was to develop a rapid, high-throughput screening method to enable genomic selection for y -60NUE in perennial ryegrass. NUE of 76 genotypes of perennial ryegrass from a breeding population were screened in a greenhouse using an automated image-based phenomics platform under low (0.5 mM) and moderate (5 mM) N levels over 3 consecutive harvests. Significant ( < 0.05) genotype, treatment, and genotype by treatment interactions for dry matter yield and NUE were observed. NUE under low and moderate N treatments was significantly correlated. Of the seven plant architecture features directly extracted from image analysis and four secondarily derived measures, mean projected plant area (MPPA) from the two side view images had the highest correlation with dry matter yield (r = 0.94). Automated digital image-based phenotyping enables temporal plant growth responses to N to be measured efficiently and non-destructively. The method developed in this study would be suitable for screening large populations of perennial ryegrass growth in response to N for genomic selection purposes.
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http://dx.doi.org/10.3389/fpls.2020.565361DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7481514PMC
August 2020

Methylglyoxal Decoration of Glutenin during Heat Processing Could Alleviate the Resulting Allergic Reaction in Mice.

Nutrients 2020 Sep 17;12(9). Epub 2020 Sep 17.

Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China.

Background: It is widely believed that Maillard reactions could affect the sensitization of allergens. However, the mechanism of action of methylglyoxal (MGO) production in Maillard reactions in the sensitization variation of glutenin (a predominant allergen in wheat) during heat processing is still unclear.

Methods: This research evaluated the effect of MGO on the immune response against glutenin in a mouse model. The resulting variations in conformation and corresponding digestibility of glutenin were determined. The immune response and gut microflora variation in mice were analyzed following administering of glutenin and MGO-glutenin.

Results: The results of the study showed that MGO-glutenin induced a lower immune response than native glutenin. Cytokine analysis showed that MGO-glutenin regulated mouse immune response by inducing Treg differentiation. MGO decoration changed the structure and digestibility of glutenin. In addition, MGO-glutenin contributes to the maintenance of the beneficial gut microflora.

Conclusion: MGO decoration of glutenin during heat processing could alleviate the resulting allergic reaction in mice. Decoration with MGO appears to contribute to the aggregation of glutenin, potentially masking surface epitopes and abating sensitization. Furthermore, Bacteroides induced regulatory T-cell (Treg) differentiation, which may contribute to inhibition of the Th2 immune response and stimulation of immune tolerance.
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http://dx.doi.org/10.3390/nu12092844DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551842PMC
September 2020

A review: Red/near-infrared (NIR) fluorescent probes based on nucleophilic reactions of H S since 2015.

Luminescence 2020 Dec 21;35(8):1156-1173. Epub 2020 Sep 21.

Department of Chemistry, Xinzhou Teachers University, Xinzhou, Shanxi, China.

The topics of human health and disease are always the focus of much attention. Hydrogen sulfide (H S), as a double-edged sword, plays an important role in biological systems. Studies have revealed that endogenous H S is important to maintain normal physiological functions. Conversely, abnormal levels of H S may contribute to various diseases. Due to the importance of H S in physiology and pathology, research into the effects of H S has been active in recent years. Fluorescent probes with red/near-infrared (NIR) emissions (620-900 nm) are more suitable for imaging applications in vivo, because of their negligible photodamage, deep tissue penetration, and maximum lack of interference from background autofluorescence. H S, an 'evil and positive' molecule, is not only toxic, but also produces significant effects; a 'greedy' molecule, is not only a strong nucleophile under physiological conditions, but also undergoes a continuous double nucleophilic reaction. Therefore, in this tutorial review, we will highlight recent advances made since 2015 in the development and application of red/NIR fluorescent probes based on nucleophilic reactions of H S.
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http://dx.doi.org/10.1002/bio.3831DOI Listing
December 2020

Colorimetric detection of glucose based on the binding specificity of a synthetic cyclic peptide.

Analyst 2020 Nov;145(22):7234-7241

State Key Laboratory of Food Nutrition and Safety, Tianjin Engineering Research Center of Safety Control Technology in Food Processing, Tianjin University of Science and Technology, Tianjin, 300457, China.

A novel colorimetric sensing method for glucose was developed based on the catalytic activity of Au nanoparticles (NPs) and a synthetic cyclic peptide that specifically binds with glucose. It is the first time that a cyclic peptide was used as a recognition element for glucose sensing. In the absence of glucose, the monolayers of cyclic peptide on the Au NP surfaces interfered little with the adsorption of 4-nitrophenol, and the Au NPs catalyze the reduction of bright yellow 4-nitrophenol to colorless 4-aminophenol in the presence of NaBH4. Added glucose was preferentially bound by the cyclic peptides and impeded the adsorption of 4-nitrophenol. Therefore, the color of the solution presented varying shades of yellow depending on the concentration of glucose. The method had a short response time of 10 min and demonstrated a linear response over a range of glucose concentrations from 0.1 mM to 20 mM, with a lower limit of detection of 0.04 mM. Meanwhile, it also provided results readily observable by the naked eye. The method was successfully applied for the detection of glucose in spiked food samples (Chinese cabbage, pear, and wheat flour) and spiked rabbit blood, and a good recovery rate of 88.04-103.28% and 94.27-101.53% was obtained, respectively.
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http://dx.doi.org/10.1039/d0an00211aDOI Listing
November 2020

Neural mechanisms of AVPR1A RS3-RS1 haplotypes that impact verbal learning and memory.

Neuroimage 2020 11 20;222:117283. Epub 2020 Aug 20.

Department of Radiology and Tianjin Key Laboratory of Functional Imaging, Tianjin Medical University General Hospital, No. 154, Anshan Road, Heping District, Tianjin 300052, China; CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China. Electronic address:

Converging evidence from both human and animal studies has highlighted the pervasive role of the neuropeptide arginine vasopressin (AVP), which is mediated by arginine vasopressin receptor 1A (AVPR1A), in both social and nonsocial learning and memory. However, the effect of genetic variants in AVPR1A on verbal learning and memory is unknown. The hippocampus is a heterogeneous structure that consists of several anatomically and functionally distinct subfields, and it is the principal target structure for the memory-enhancing effect of AVP. We tested the hypothesis that genetic variants in the RS3 and RS1 repeat polymorphisms may influence verbal learning and memory performance evaluated by the California Verbal Learning Test-II (CVLT-II) by modulating the gray matter volume (GMV) and resting-state functional connectivity (rsFC) of whole hippocampus and its subfields in a large cohort of young healthy subjects (n = 1001). Using a short/long classification scheme for the repeat length of RS3 and RS1, we found that the individuals carrying more short alleles of RS3-RS1 haplotypes had poorer learning and memory performance compared to that of those carrying more long alleles. We also revealed that individuals carrying more short alleles exhibited a significantly smaller GMV in the left cornu ammonis (CA)2/3 and weaker rsFC of the left CA2/3-bilateral thalamic (primarily in medial prefrontal subfields) compared to those carrying more long alleles. Furthermore, multiple mediation analysis confirmed that these two hippocampal imaging measures jointly and fully mediated the relationship between the genetic variants in AVPR1A RS3-RS1 haplotypes and the individual differences in verbal learning and memory performance. Our results suggest that genetic variants in AVPR1A RS3-RS1 haplotypes may affect verbal learning and memory performance in part by modulating the left hippocampal CA2/3 structure and its rsFC with the thalamus.
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http://dx.doi.org/10.1016/j.neuroimage.2020.117283DOI Listing
November 2020

Platelet-derived PDGF promotes the invasion and metastasis of cholangiocarcinoma by upregulating MMP2/MMP9 expression and inducing EMT via the p38/MAPK signalling pathway.

Am J Transl Res 2020 15;12(7):3577-3595. Epub 2020 Jul 15.

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University Chongqing 400038, China.

Cholangiocarcinoma (CCA) is an aggressive tumour with a poor prognosis due to its late clinical presentation and the lack of effective non-surgical therapies. Previous studies have reported that platelets are implicated in tumour invasion and metastasis, while their role and the underlying mechanism in CCA remain unclear. Here, we show that platelets are hyperactivated in patients with CCA and that platelet-derived growth factor (PDGF) promotes the migration of CCA tumour cells both in vitro and in vivo. Further investigations revealed that PDGF can upregulate the expression of MMP2/MMP9 and induce epithelial-mesenchymal transition (EMT) by activating the p38/MAPK signalling pathway in CCA cells. In addition, the expression of MMP2/MMP9 was associated with lymph node metastasis and poor prognosis in CCA patients after surgical resection. In conclusion, our findings demonstrate that platelets play an important role in facilitating the invasion and metastasis of CCA cells by secreting PDGF, which may provide a novel target for CCA treatment.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407735PMC
July 2020

Right Posterior Insula and Putamen Volume Mediate the Effect of Oxytocin Receptor Polygenic Risk for Autism Spectrum Disorders on Reward Dependence in Healthy Adults.

Cereb Cortex 2021 Jan;31(2):746-756

Department of Radiology and Tianjin Key Laboratory of Functional Imaging, Tianjin Medical University General Hospital, Tianjin 300052, China.

Much evidence indicates the influence of the oxytocin receptor (OXTR) gene on autism spectrum disorders (ASDs), a set of disorders characterized by a range of deficits in prosocial behaviors, which are closely related to the personality trait of reward dependence (RD). However, we do not know the effect of the OXTR polygenic risk score for ASDs (OXTR-PRSASDs) on RD and its underlying neuroanatomical substrate. Here, we aimed to investigate associations among the OXTR-PRSASDs, gray matter volume (GMV), and RD in two independent datasets of healthy young adults (n = 450 and 540). We found that the individuals with higher OXTR-PRSASDs had lower RD and significantly smaller GMV in the right posterior insula and putamen. The GMV of this region showed a positive correlation with RD and a mediation effect on the association between OXTR-PRSASDs and RD. Moreover, the correlation map between OXTR-PRSASDs and GMV showed spatial correlation with OXTR gene expression. All results were highly consistent between the two datasets. These findings highlight a possible neural pathway by which the common variants in the OXTR gene associated with ASDs may jointly impact the GMV of the right posterior insula and putamen and further affect the personality trait of RD.
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http://dx.doi.org/10.1093/cercor/bhaa198DOI Listing
January 2021

Rapid Detection of Kaempferol Using Surface Molecularly Imprinted Mesoporous Molecular Sieves Embedded with Carbon Dots.

Int J Anal Chem 2020 10;2020:5819062. Epub 2020 Jul 10.

Medical College, Nankai University, Tianjin 300350, China.

This work demonstrates rapid sensing of kaempferol using active sensing material synthesized using the one-pot surface-imprinting synthesis method. This sensor consisted of molecularly imprinted polymer (MIP) consisting of mesoporous molecular sieves (SBA-15) loaded with carbon dots (CDs). Fourier transform infrared (FT-IR) spectroscopy confirmed successful incorporation of CDs onto the surface of imprinted mesoporous molecular sieves. Ordered hexagonal arrays of [email protected]@MIP mesopore structure were confirmed with transmission electron microscopy. Fluorescence intensity of [email protected]@MIP composites linearly correlated with kaempferol content in the 0.05-2 mg/L range. Detection limit was 14 g/L. MIPs were used for efficient detection of kaempferol in fruit and vegetable samples with recovery values from 80% to 112%. The method has high sensitivity, low cost, good selectivity, and many application potentials useful for research and development of flavonoid monomer presence in food.
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http://dx.doi.org/10.1155/2020/5819062DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368943PMC
July 2020

Irradiation pretreatment enhances the therapeutic efficacy of platelet-membrane-camouflaged antitumor nanoparticles.

J Nanobiotechnology 2020 Jul 20;18(1):101. Epub 2020 Jul 20.

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury of PLA, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing, 400038, China.

Background: Cell membrane-based nanocarriers are promising candidates for delivering antitumor agents. The employment of a simple and feasible method to improve the tumor-targeting abilities of these systems is appealing for further application. Herein, we prepared a platelet membrane (PM)-camouflaged antitumor nanoparticle. The effects of irradiation pretreatment on tumor targeting of the nanomaterial and on its antitumor action were evaluated.

Results: The biomimetic nanomaterial constructed by indocyanine green, poly(d,l-lactide-co-glycolide), and PM is termed [email protected] A 4-Gy X-ray irradiation increased the proportions of G2/M phase and Caveolin-1 content in 4T1 breast cancer cells, contributing to an endocytic enhancement of [email protected] [email protected] produced hyperthermia and reactive oxygen species upon excitation by near-infrared irradiation, which were detrimental to the cytoplasmic lysosome and resulted in cell death. Irradiation pretreatment thus strengthened the antitumor activity of [email protected] in vitro. Mice experiments revealed that irradiation enhanced the tumor targeting capability of [email protected] in vivo. When the same dose of [email protected] was intravenously administered, irradiated mice had a better outcome than did mice without X-ray pretreatment.

Conclusion: The study demonstrates an effective strategy combining irradiation pretreatment and PM camouflage to deliver antitumor nanoparticles, which may be instrumental for targeted tumor therapy.
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http://dx.doi.org/10.1186/s12951-020-00660-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7372815PMC
July 2020

A fluorescence quenching-recovery sensor based on RCA for the specific analysis of Fusobacterium nucleatum. nucleatum.

Anal Biochem 2020 09 3;604:113808. Epub 2020 Jul 3.

State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, 300457, China. Electronic address:

Fusobacterium nucleatum. nucleatum (Fn.n) is associated with colorectal carcinoma. A highly sensitive fluorescence quenching-recovery detection platform based on rolling circle amplification and hairpin molecular beacon technology for the specific analysis of Fn.n, hairpin MB being used to achieve double quenching. First, a specific recognition sequence target in the padlock probe was designed based on the nusG specific gene of Fn.n. The padlock probe then formed circular DNA using the ligase. After digestion, linear amplification was achieved by the phi29 DNA polymerase and the RCA primer, and a large amount of amplified products was obtained. Subsequently, the amplification products hybridized with the signal probe, leading to the opening of the hairpin structure in MBs. As a result, FAM at 5' end and BHQ-1 at 3' end became separated, which allowed for the recovery of the fluorescence signal. The proposed method showed high sensitivity and specificity for the detection of Fn.n genomic DNA (LOD as low as 0.7 ng L) and performed well in the identification of this bacteria in real samples.
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http://dx.doi.org/10.1016/j.ab.2020.113808DOI Listing
September 2020

SRC-3 Functions as a Coactivator of T-bet by Regulating the Maturation and Antitumor Activity of Natural Killer Cells.

Cancer Immunol Res 2020 09 19;8(9):1150-1162. Epub 2020 Jun 19.

State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, Chongqing Engineering Research Center for Nanomedicine, College of Preventive Medicine, Third Military Medical University, Chongqing, China.

Natural killer (NK)-cell development and maturation is a well-organized process. The steroid receptor coactivator 3 (SRC-3) is a regulator of the hematopoietic and immune systems; however, its role in NK cells is poorly understood. Here, SRC-3 displayed increased nuclear translocation in NK cells during terminal differentiation and upon inflammatory cytokine stimulation. Targeted deletion of SRC-3 altered normal NK-cell distribution and compromised NK-cell maturation. SRC-3 deficiency led to significantly impaired NK-cell functions, especially their antitumor activity. The expression of several critical T-bet target genes, including , and , but not T-bet itself, was markedly decreased in NK cells in the absence of SRC-3. There was a physiologic interaction between SRC-3 and T-bet proteins, where SRC-3 was recruited by T-bet to regulate the transcription of the aforementioned genes. Collectively, our findings unmask a previously unrecognized role of SRC-3 as a coactivator of T-bet in NK-cell biology and indicate that targeting SRC-3 may be a promising strategy to increase the tumor surveillance function of NK cells.
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http://dx.doi.org/10.1158/2326-6066.CIR-20-0181DOI Listing
September 2020

Cys-functionalized AuNP substrates for improved sensing of the marine toxin STX by dynamic surface-enhanced Raman spectroscopy.

Anal Bioanal Chem 2020 Jul 17;412(19):4609-4617. Epub 2020 Jun 17.

Anhui Province Key Laboratory of Medical Physics and Technology, Center of Medical Physics and Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, Anhui, China.

Saxitoxin (STX) as one of the most harmful and typical paralytic shellfish toxins, is posing a serious threat to environmental and human health, thus it is essential to develop a sensitive and reliable analytical method for STX detection. Herein, we proposed a strategy for rapid and sensitive detection of STX with surface-enhanced Raman spectroscopy (SERS), by employing cysteine modified gold nanoparticles (Cys-AuNPs) as SERS probe to capture STX molecules through electrostatic interactions and multiple hydrogen bonds between Cys and STX molecules. Moreover, the XPS and zeta potential results indicated that Cys could bond to AuNPs through Au-S bonds and the addition of STX could induce the efficient aggregation of Cys-AuNPs owing to the presence of electrostatic interactions and multiple hydrogen bonds between Cys and STX molecules. Furthermore, considering the high sensitivity and stability of the dynamic surface-enhanced Raman spectroscopy (D-SERS) strategy with the formation of a 3D hotspot matrix, the highly sensitive detection of STX was realized to a level of 1 × 10 M by using the D-SERS strategy. Consequently, Cys-AuNPs as high affinity substrates can provide high sensitivity for the detection of STX through the D-SERS strategy. Graphical abstract.
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http://dx.doi.org/10.1007/s00216-020-02710-9DOI Listing
July 2020

Development and Validation of a Phenotyping Computational Workflow to Predict the Biomass Yield of a Large Perennial Ryegrass Breeding Field Trial.

Front Plant Sci 2020 28;11:689. Epub 2020 May 28.

Agriculture Victoria, Hamilton Centre, Hamilton, VIC, Australia.

Increasing dry matter yield (DMY) is the most important objective in perennial ryegrass breeding programs. Current yield assessment methods like cutting are time-consuming and destructive, non-destructive measures such as scoring yield on single plants by visual inspection may be subjective. These assessments involve multiple measurements and selection procedures across seasons and years to evaluate biomass yield repeatedly. This contributes to the slow process of new cultivar development and commercialisation. This study developed and validated a computational phenotyping workflow for image acquisition, processing and analysis of spaced planted ryegrass and investigated sensor-based DMY yield estimation of individual plants through normalized difference vegetative index (NDVI) and ultrasonic plant height data extraction. The DMY of 48,000 individual plants representing 50 advanced breeding lines and commercial cultivars was accurately estimated at multiple harvests across the growing season. NDVI, plant height and predicted DMY obtained from aerial and ground-based sensors illustrated the variation within and between cultivars across different seasons. Combining NDVI and plant height of individual plants was a robust method to enable high-throughput phenotyping of biomass yield in ryegrass breeding. Similarly, the plot-level model indicated good to high-coefficients of determination () between the predicted and measured DMY across three seasons with between 0.19 and 0.81 and root mean square errors (RMSE) values ranging from 0.09 to 0.21 kg/plot. The model was further validated using a combined regression of the three seasons harvests. This study further sets a foundation for the application of sensor technologies combined with genomic studies that lead to greater rates of genetic gain in perennial ryegrass biomass yield.
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http://dx.doi.org/10.3389/fpls.2020.00689DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7270830PMC
May 2020