Publications by authors named "Junmei Zhao"

35 Publications

O3-NaFeNiMnAlO Cathodes with Improved Air Stability for Na-Ion Batteries.

ACS Appl Mater Interfaces 2021 Jul 9;13(28):33015-33023. Epub 2021 Jul 9.

Key Laboratory for Renewable Energy, Beijing Key Laboratory for New Energy Materials and Devices, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.

Na-ion batteries (NIBs) have been considered as potential candidates for large-scale energy storage, where O3-type Na-based layered oxide cathodes have attracted great attention due to their high capacity and low cost. However, O3-NaTMO materials still suffer from insufficient air stability, which could lead to deteriorative electrochemical properties and thus hinder their practical application. In this work, a series of Al-doped O3-NaFeNiMnAlO cathodes prepared by a co-precipitation method were investigated to enhance their electrochemical performance and air stability through stabilizing their structural and interface chemical properties. The Al-doped O3NaFeNiMnAlO (NFNMA) cathode delivers a comparable capacity of 138 mAh g and keeps a capacity retention of 85.88% after 50 cycles at 0.2 C, while the undoped O3NaFeNiMnO (NFNM) can only keep a capacity retention of 71.02%, although with an initial capacity of 141 mAh g at 0.2 C. For the air stability, the capacity decay rates are 58.87 and 5.07% for the undoped NFNM and Al-doped NFNMA after the air exposure for 30 days, respectively. The greatly decaying electrochemical performance could be due to the formation of carbonates during air exposure, which can be efficiently suppressed by Al doping. The doped Al has been confirmed to be inserted into the NFNM crystal lattice, inducing the reduced values of lattice parameters and due to the smaller ionic radius of Al (53.5 pm) vs Fe (55.0 pm). This study demonstrates that Al doping plays an important role in the air stability and cycling stability for layered cathode materials, which offers an efficient strategy to optimize the material design for their practical application in NIBs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c07554DOI Listing
July 2021

Human Neutrophil Elastase Mediates MUC5AC Hypersecretion via the Tumour Necrosis Factor-α Converting Enzyme-Epidermal Growth Factor Receptor Signalling Pathway in vivo.

ORL J Otorhinolaryngol Relat Spec 2021 Jun 15:1-9. Epub 2021 Jun 15.

Department of Otolaryngology, The First Affiliated Hospital of Nanchang University, Nanchang, China.

Objectives: The objective of this study is to examine the role of the tumour necrosis factor-α converting enzyme-epidermal growth factor receptor (TACE-EGFR) pathway in human neutrophil elastase (HNE)-induced MUC5AC mucin expression in mice.

Method: Four groups of mice, treated with HNE alone (HNE group), HNE plus TACE inhibitor (HNE + TAPI-2 group), HNE plus EGFR inhibitor (HNE + AG1478 group), and untreated (control group), were used in the experiment. Histopathological changes were monitored by haematoxylin-eosin (HE) and periodic acid-Schiff (PAS) staining. TACE, EGFR, and MUC5AC expression in the nasal mucosa were determined using immunohistochemistry. The expression of p-EGFR, EGFR, and TACE protein was analysed on Western blots, and MUC5AC protein levels were assessed via ELISA. TACE, EGFR, and MUC5AC expression in the nasal mucosa were determined using real-time quantitative PCR.

Results: Compared to the control group, HE-stained tissues from the HNE group showed an irregular epithelium as well as goblet cell and submucosal glandular hyperplasia. In the nasal mucosa, strongly positive fuchsia granules were seen in PAS staining and significant increases in TACE, EGFR, MUC5AC mRNA, and protein expression were detected (p < 0.01). The HNE + TAPI-2 and HNE + AG1478 groups had significantly less goblet cell and submucosal gland hyperplasia as well as weaker PAS staining. Compared to mice treated with HNE alone, in HNE + TAPI-2-treated mice, the levels of TACE, EGFR, and MUC5AC mRNA and protein as well as p-EGFR protein were significantly reduced (p < 0.01). In HNE + AG1478-treated mice, EGFR and MUC5AC mRNA and protein levels and p-EGFR protein expression were reduced significantly (p < 0.01), but the difference in TACE mRNA and protein expression between the HNE + AG1478 and HNE groups was not significant (p > 0.05).

Conclusion: Using a newly developed, stable experimental model of nasal hypersecretion in mice, we showed that TAPI-2 or AG1478 inhibited HNE-induced MUC5AC production. This suggests that MUC5AC mucin expression in vivo is mediated by a cascade involving the HNE-TACE-EGFR signalling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1159/000509982DOI Listing
June 2021

Rapid mechanochemical synthesis of polyanionic cathode with improved electrochemical performance for Na-ion batteries.

Nat Commun 2021 May 14;12(1):2848. Epub 2021 May 14.

Key Laboratory for Renewable Energy, Beijing Key Laboratory for New Energy Materials and Devices, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, China.

Na-ion batteries have been considered promising candidates for stationary energy storage. However, their wide application is hindered by issues such as high cost and insufficient electrochemical performance, particularly for cathode materials. Here, we report a solvent-free mechanochemical protocol for the in-situ fabrication of sodium vanadium fluorophosphates. Benefiting from the nano-crystallization features and extra Na-storage sites achieved in the synthesis process, the as-prepared carbon-coated Na(VOPO)F nanocomposite exhibits capacity of 142 mAh g at 0.1C, higher than its theoretical capacity (130 mAh g). Moreover, a scaled synthesis with 2 kg of product was conducted and 26650-prototype cells were demonstrated to proof the electrochemical performance. We expect our findings to mark an important step in the industrial application of sodium vanadium fluorophosphates for Na-ion batteries.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41467-021-23132-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8121810PMC
May 2021

Influence of feeding thermally peroxidized lipids on growth performance, lipid digestibility, and oxidative status in nursery pigs.

J Anim Sci 2020 Dec;98(12)

Cargill Animal Nutrition, Elk River, MN.

Three experiments were conducted to evaluate oil source and peroxidation status (experiment 1) or peroxidized soybean oil (SO; experiments 2 and 3) on growth performance, oxidative stress, and digestibility of dietary ether extract (EE). In experiment 1, palm oil (PO), poultry fat (PF), canola oil (CO), and SO were evaluated, while in experiments 2 and 3, only SO was evaluated. Lipids were either an unheated control (CNT) or thermally processed at 90 °C for 72 hr, being added at 10%, 7.5%, or 3% of the diet in experiments 1, 2, and 3, respectively. In experiment 1, 288 pigs (body weight, BW, 6.1 kg) were fed 1 of 8 factorially arranged treatments with the first factor being lipid source (PO, PF, CO, and SO) and the second factor being peroxidation status (CNT or peroxidized). In experiment 2, 216 pigs (BW 5.8 kg) were fed 1 of 6 treatments consisting of 100%, 90%, 80%, 60%, 20%, and 0% CNT SO blended with 0%, 10%, 20%, 40%, 80%, and 100% peroxidized SO, respectively. In experiment 3, 72 pigs (BW 5.8 kg) were fed either CNT or peroxidized SO. Pigs were fed 21 d with feces collected on day 12 or 14 and pigs bled on day 12 blood collection. In experiment 1, an interaction between oil source and peroxidation status was observed for averaged daily gain (ADG) and average daily feed intake (ADFI; P = 0.10) which was due to no impact of feeding pigs peroxidized PO, PF, or SO on ADG or ADFI compared with feeding pigs CNT PO, PF, or SO, respectively; while pigs fed peroxidized CO resulted in reduced ADG and ADFI compared with pigs fed CNT CO. There was no interaction between oil source and peroxidation status, and no lipid source effect on gain to feed ratio (GF; P ≥ 0.84), but pigs fed the peroxidized lipids had a lower GF compared with pigs fed the CNT lipids (P = 0.09). In experiment 2, feeding pigs diets containing increasing levels of peroxidized SO resulted in reduced ADG (quadratic, P = 0.03), ADFI (linear, P = 0.01), and GF (quadratic, P = 0.01). In experiment 3, feeding peroxidized SO at 3% of the diet reduced ADG (P = 0.11) and ADFI (P = 0.13), with no observed change in GF (P = 0.62). Differences in plasma protein carbonyls, glutathione peroxidase, and vitamin E due to feeding peroxidized lipids were inconsistent across the 3 experiments. Digestibility of dietary EE was reduced in pigs fed peroxidized PO or SO (P = 0.01, experiment 1) and peroxidized SO in experiments 2 and 3 (P ≤ 0.02). In conclusion, the peroxidation status of dietary lipids consistently affects growth performance and EE digestibility but has a variable effect on measures of oxidative stress.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/jas/skaa392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8218929PMC
December 2020

Revisiting of Tetragonal NaVPOF: A High Energy Density Cathode for Sodium-Ion Batteries.

ACS Appl Mater Interfaces 2020 Jul 26;12(27):30510-30519. Epub 2020 Jun 26.

Division of Energy Storage, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Zhongshan Road 457, Dalian 116023, People's Republic of China.

Tetragonal NaVPOF has been regarded as an ideal cathode for sodium-ion batteries because of its high average plateau (3.8 V) and theoretical specific capacity (143 mA h g). However, the Na-storage performance is still hindered by unsatisfying thermal stability and poor conductivity. Herein, a stable tetragonal NaVPOF has been synthesized by a novel solvent thermal method using a carbon coating precursor. The as-prepared [email protected] nanocomposite delivers a capacity of 133 mA h g at 0.2 C, corresponding to an excellent energy density of 509 W h kg; when coupled with an HC anode, the full cell still displays an outstanding performance of 124 mA h g at 0.05 C. Fast Na diffusion kinetics ( = 10 to 10 cm s) and small volume change (4.4%) are exploited, which ensures good rate trait and cycling stability of tetragonal NaVPOF. Further, the Na extraction-insertion mechanism has been explored by analyzing the crystal structure change during in situ X-ray powder diffraction cycles.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.0c08846DOI Listing
July 2020

Selective recovery of Li and FePO from spent LiFePO cathode scraps by organic acids and the properties of the regenerated LiFePO.

Waste Manag 2020 Jul 3;113:32-40. Epub 2020 Jun 3.

CAS Key Laboratory of Green Process Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Science, No. 1 BeiErTiao, Zhong Guan Cun, Beijing 100190, China; Innovation Academy for Green Manufacture, Chinese Academy of Sciences, Beijing 100190, China. Electronic address:

To recycle the sharply growing spent lithium-ion batteries and alleviate concerns over shortages of resources, particularly Li, is still an urgent issue. In this work, an organic acids based leaching approach at room temperature is proposed to recover Li and FePO from spent LiFePO cathode powder. The coexistent metal ions, Cu and Al, have also been investigated. Citrus fruit juices, rich in organic acids, such as citric acid and malic acid, have been used as leaching agents in this work. Among lemon, orange and apple, lemon juice shows the best leaching effect based on its suitable pH of the reaction system. Under the optimized conditions, the leaching rates of Li, Cu and Al can reach up to 94.83%, 96.92% and 47.24%, while Fe and P remain as low as 4.05% and 0.84%, respectively. LiCO and FePO can be recovered from the leachate and the leaching residue, respectively. The recovered FePO was used to prepare new cathode material LiFePO. The crystalline carbon, present in the spent LiFePO cathode scraps, has a significant effect on the electrochemical performances of the regenerated LiFePO. The regenerated LiFePO cathode material delivered a comparable discharge capacity of 155.3 mAh g at 0.1C and rate capacity to the fresh LiFePO. For the cycling stability, it displays capacity retention of 98.30% over 100 cycles at 1 C with a fading rate of 0.017% per cycle. The proposed organic acids-based recycling strategy is much benign for recycling the spent LiFePO cathode materials.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.wasman.2020.05.046DOI Listing
July 2020

MondoA Is Required for Normal Myogenesis and Regulation of the Skeletal Muscle Glycogen Content in Mice.

Diabetes Metab J 2021 May 18;45(3):439-451. Epub 2020 May 18.

Department of Endocrinology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Background: Skeletal muscle is the largest tissue in the human body, and it plays a major role in exerting force and maintaining metabolism homeostasis. The role of muscle transcription factors in the regulation of metabolism is not fully understood. MondoA is a glucose-sensing transcription factor that is highly expressed in skeletal muscle. Previous studies suggest that MondoA can influence systemic metabolism homeostasis. However, the function of MondoA in the skeletal muscle remains unclear.

Methods: We generated muscle-specific MondoA knockout (MAKO) mice and analyzed the skeletal muscle morphology and glycogen content. Along with skeletal muscle from MAKO mice, C2C12 myocytes transfected with small interfering RNA against MondoA were also used to investigate the role and potential mechanism of MondoA in the development and glycogen metabolism of skeletal muscle.

Results: MAKO caused muscle fiber atrophy, reduced the proportion of type II fibers compared to type I fibers, and increased the muscle glycogen level. MondoA knockdown inhibited myoblast proliferation, migration, and differentiation by inhibiting the phosphatase and tensin homolog (PTEN)/phosphoinositide 3-kinase (PI3K)/Akt pathway. Further mechanistic experiments revealed that the increased muscle glycogen in MAKO mice was caused by thioredoxin-interacting protein (TXNIP) downregulation, which led to upregulation of glucose transporter 4 (GLUT4), potentially increasing glucose uptake.

Conclusion: MondoA appears to mediate mouse myofiber development, and MondoA decreases the muscle glycogen level. The findings indicate the potential function of MondoA in skeletal muscle, linking the glucose-related transcription factor to myogenesis and skeletal myofiber glycogen metabolism.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.4093/dmj.2019.0212DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164950PMC
May 2021

Retinoic acid inducible gene-I slows down cellular senescence through negatively regulating the integrin β3/p38 MAPK pathway.

Cell Cycle 2019 Dec 9;18(23):3378-3392. Epub 2019 Oct 9.

State Key Laboratory for Medical Genomics, Shanghai Institute of Hematology and Collaborative Innovation Center of Hematology, Rui-Jin Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai, China.

Retinoic acid inducible gene-I (Rig-I) has been well documented as a cytosolic pattern recognition receptor that can sense viral RNA ligands to initiate the interferon-mediated antiviral immunity. However, little is known about the biological behaviors of Rig-I devoid of viral infection. Herein, we investigated the roles of Rig-I in the regulation of cellular senescence. In comparison to wild-type counterparts, mice displayed the accelerated loss of hair, less responsiveness to gentle physical stimuli and shorten survival time. Likewise, Rig-I deficiency rendered mouse embryonic fibroblasts (MEFs) more susceptible to the serial passages-associated replicative senescence. By performing a transcriptome analysis, we identified integrins at the intersections of biological pathways affected by Rig-I. Among these, integrin β3 was negatively regulated by Rig-I, and significantly upregulated with the occurrence of senescence. Gene silencing of Itgb3 (encoding integrin β3) retarded the progression of cellular senescence in both WT and MEFs. Notably, this effect was more prominent in MEFs. Furthermore, p38 MAPK was a key downstream molecule for integrin β3-mediated senescence, and overactivated in senescent MEFs. Taken together, Rig-I deficiency contributes to cellular senescence through amplifying integrin β3/p38 MAPK signaling. Our findings provide the evidence that Rig-I is a key regulator of cellular senescence, which will be helpful in better understanding its function without viral infection. Rig-I: retinoic acid inducible gene-I; SASP: senescence-associated secretory phenotype; ECM: extracellular matrix; Itgb3: integrin beta 3; PRR: pattern recognition receptor; MEFs: mouse embryonic fibroblasts; Il-1β: interleukin-1 beta; Il-6: interleukin-6; Il-8: interleukin-8; Cxcl1: chemokine (C-X-C motif) ligand 1; Ccl2: chemokine (C-C motif) ligand 2; WT, wild type; BM: bone marrow; MAPK: mitogen-activated protein kinase; ERK: extracellular signal-regulated kinases; JNK: Jun N-terminal kinases; SA-β-gal: senescence-associated β-galactosidase; qPCR: quantitative reverse-transcription PCR; PBS: phosphate-buffered saline.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1080/15384101.2019.1677074DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6927694PMC
December 2019

Human Neutrophil Elastase Induces MUC5AC Overexpression in Chronic Rhinosinusitis Through miR-146a.

Am J Rhinol Allergy 2020 Jan 29;34(1):59-69. Epub 2019 Aug 29.

Department of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, P.R. China.

View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/1945892419871798DOI Listing
January 2020

The role of autophagy in the overexpression of MUC5AC in patients with chronic rhinosinusitis.

Int Immunopharmacol 2019 Jun 22;71:169-180. Epub 2019 Mar 22.

Department of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, Jiangxi, PR China. Electronic address:

Background: Autophagy is a lysosomal degradation pathway that protects the body and is essential for cell survival and differentiation. Mucins (MUCs) are important components of secreted mucus, mucin (MUC)5 AC is the major MUC secreted in the normal airway.

Objective: Investigated the role of autophagy in pathogenic mucin (MUC)5 AC production during chronic rhinosinusitis (CRS).

Methods: The expression of human neutrophil elastase (HNE) and the autophagic proteins microtubule-associated protein 1 light chain (LC)3B-II, c-Jun N-terminal kinase (JNK), c-Jun, and MUC5AC were analyzed in the sinonasal mucosa and human nasal epithelial cells (HNECs) using immunohistochemistry, enzyme-linked immunosorbent assay (ELISA), and quantitative real-time polymerase chain reaction (qRT-PCR). Autophagic vacuoles were studied using transmission electron microscopy (TEM). Primary HNECs were treated with HNE, bafilomycin A1, and SP600125. In some experiments, cultured primary HNECs were transfected with small interfering RNAs (siRNAs) to target Beclin-1 (BECN1; BECN1-siRNA), autophagy-related gene 5 (Atg5; Atg5-siRNA), and c-Jun (c-Jun-siRNA). Cultured cells were analyzed using western blotting, qRT-PCR, and ELISA.

Results: In CRS patients, both with and without nasal polyps, the expression levels of HNE, LC3B, JNK, c-Jun, and MUC5AC were upregulated. Bafilomycin A1 upregulated LC3B-II expression and inhibited MUC secretion in HNE-treated normal primary HNECs. Autophagosomes were observed in HNE-treated primary HNECs using TEM. HNE-induced secretion of MUC5AC was suppressed in normal primary HNECs by BECN1-siRNA, Atg5-siRNA, c-Jun-siRNA, and SP600125.

Conclusions: In HNE-induced CRS, autophagy increases the secretion of MUC5AC by promoting the phosphorylation of JNK and c-Jun.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.intimp.2019.03.028DOI Listing
June 2019

Effect of mGluR7 on proliferation of human embryonic neural stem cells.

Medicine (Baltimore) 2019 Mar;98(9):e14683

Department of Clinical Medicine, Medical College of Yan'an University, Yan'an, P. R. China.

This study is to investigate the effect of metabotropic glutamate receptor 7 (mGluR7) on the proliferation of human embryonic neural stem cells (NSCs) and its molecular mechanism.Human embryonic NSCs were isolated. The pCMV2-GV146-GFP-mGluR7 plasmid was transfected to over-express mGluR7 while mGluR7 siRNA was transfected to knockdown mGluR7. MTT assay was used to analyze cell proliferation. Flow cytometry was used to detect cell cycle and apoptosis. Protein and mRNA levels were analyzed by Western blot and RT-qPCR, respectively.The viability of human NSCs and the diameter of neurospheres after 24 hours, 48 hours, and 72 hours of transfection significantly increased by mGluR7 overexpression whereas significantly decreased by mGluR7 knockdown. Ki-67 expression was up-regulated by mGluR7 overexpression whereas down-regulated by mGluR7 siRNA, indicating a promotive effect of mGluR7 on NSC proliferation. After mGluR7 overexpression, G1/G0 phase cell ratio dropped significantly compared with control group, while the S phase cell ratio increased. mGluR7 silencing arrested human NSCs at G1/G0 phase. After 48 hours of transfection, there was a decrease of apoptosis by mGluR7 overexpression, while mGluR7 silencing induced apoptosis of human NSCs. Additionally, overexpression of mGluR7 up-regulated the expression of p-serine/threonine kinase (AKT), cyclin D1, and cyclin-dependent kinase 2 (CDK2). The mGluR7 knockdown had opposite effects. Similarly, mGluR7 down-regulated the expression of Caspase-3/9, while the mGluR7 knockdown promoted this.mGluR7 can promote the proliferation of human embryonic cortical NSCs in vitro. This effect may be mediated by promoting cell cycle progression, inhibiting cell apoptosis, activating the AKT signaling pathway, and inhibiting the Caspase-3/9 signaling pathway.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000014683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6831331PMC
March 2019

Lyophilizing thrombin powder-based treatment for hemostasis during coil embolization of ruptured cerebral aneurysm: Two case reports.

Interv Neuroradiol 2019 Aug 25;25(4):454-459. Epub 2019 Feb 25.

2 Department of Interventional Neuroradiology, Beijing Neurosurgical Institute and Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Background: Rupture of cerebral aneurysm is an inevitable complication during embolization, followed by subsequent acute subarachnoid hemorrhage or intracranial hematoma, and results in the aggravation of a patient's condition. In particular, for patients who have had a ruptured aneurysm, urgent treatment strategies are required during operation. The most common hemostatic methods seen in clinical practices are as follows: after lowering the blood pressure, we continue to embolize the aneurysms with detachable coils as soon as possible or inject with Glubran/Onyx embolization liquids, as well as use a balloon catheter to temporarily block the blood supply. If the conditions are permissible, a balloon guiding catheter may even be used to restrict the proximal blood flow. At times, due to limitations of these methods, neurosurgeons are requested to perform craniotomy to treat the hemostasis. However, the delayed transition often leads to rapid deterioration of the patient's condition and even death due to cerebral hernia.

Case Description: We herein presented two cases of ruptured cerebral aneurysms to provide an alternative method for hemostasis and to save the lives of patients as much as possible. In an extremely urgent situation (conventional treatment is ineffective), we successfully saved the patient's life by injecting lyophilizing thrombin powder (LTP) solution into the aneurysmal sac and the parent artery through a microcatheter.

Conclusions: To our knowledge, this is the first report of successful hemostasis during coil embolization of ruptured cerebral aneurysm with LTP. Further prospective studies are needed to confirm the safety and efficacy of LTP in cerebrovascular interventional therapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/1591019918824866DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6607606PMC
August 2019

Proteomic Screening for Serum Biomarkers for Cervical Cancer and Their Clinical Significance.

Med Sci Monit 2019 Jan 9;25:288-297. Epub 2019 Jan 9.

Department of Clinical Medicine, Medical College of Yan'an University, Yan'an, Shaanxi, China (mainland).

BACKGROUND The present study aimed to determine serum markers for cervical cancer (CC) and to provide valuable references for clinical diagnosis and treatment. MATERIAL AND METHODS Serum samples were collected from age-matched healthy control women, and from female CC patients before and after surgery. Serum biomarkers were selected by comparing serum peptides profiles among the 3 groups by magnetic bead-based weak cation - exchange chromatography fractionation combined with matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Probable serum biomarkers for cervical cancer were then further identified by liquid chromatography-electrospray ionization-tandem mass spectrometry system and the identified proteins were verified by enzyme-linked immunosorbent assay (ELISA). RESULTS Three peptide biomarkers were identified for distinguishing CC patients from normal individuals, and distinguishing preoperative CC patients from postoperative CC patients. Of these 3 identified protein peptide regions, 2 peptide regions - TKT (Peak 2, 2435.63 m/z, 499-524) and FGA (Peak 4, 2761.79 m/z, 603-629) - were identified as upregulated markers, and peptide region of APOA1 (Peak 9, 2575.3 m/z, 245-260) was identified as a downregulated biomarker in preoperative CC patients compared with healthy women. CONCLUSIONS The present study provides a new method for identifying potential serum biomarkers for CC patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.12659/MSM.911478DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338008PMC
January 2019

Inactivation of PBX3 and HOXA9 by down-regulating H3K79 methylation represses NPM1-mutated leukemic cell survival.

Theranostics 2018 30;8(16):4359-4371. Epub 2018 Jul 30.

State Key Laboratory for Medical Genomics, Shanghai Institute of Hematology, Rui-Jin Hospital affiliated to Shanghai Jiao-Tong University School of Medicine, Shanghai, China.

Acute myeloid leukemia (AML) with an NPM1 mutation (NPMc+) has a distinct gene expression signature and displays molecular abnormalities similar to mixed lineage leukemia (MLL), including aberrant expression of the and gene cluster. However, it is unclear if the aberrant expression of PBX3 and HOXA is essential for the survival of NPM1-mutated leukemic cells. Using the gene expression profiling of TCGA and E-MTAB-3444 datasets, we screened for high co-expression of PBX3 and HOXA9 in NPMc+ leukemia patients. We performed NPMc+ depletion and overexpression experiments to examine aberrant H3K79 methylation through epigenetic regulation. Through RNA interference technology and small-molecule inhibitor treatment, we evaluated the effect of methyl-modified H3K79 on cell survival and explored the possible underlying mechanism. We showed that NPMc+ increased the expression of PBX3 and HOXA9, which are both poor prognosis indicators in AML. High PBX3 and HOXA9 expression was accompanied by increased dimethylated and trimethylated H3K79 in transgenic murine LinSca-1c-Kit cells and human NPMc+ leukemia cells. Using chromatin immunoprecipitation sequencing (ChIP-seq) assays of NPMc+ cells, we determined that hypermethylated H3K79 was present at the expressed gene but not the gene. PBX3 expression was positively regulated by HOXA9, and a reduction in either PBX3 or HOXA9 resulted in NPMc+ cell apoptosis. Importantly, an inhibitor of DOT1L, EPZ5676, effectively and selectively promoted NPMc+ human leukemic cell apoptosis by reducing HOXA9 and PBX3 expression. Our data indicate that NPMc+ leukemic cell survival requires upregulation of PBX3 and HOXA9, and this action can be largely attenuated by a DOT1L inhibitor.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7150/thno.26900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134928PMC
September 2019

MECP2 expression in gastric cancer and its correlation with clinical pathological parameters.

Medicine (Baltimore) 2017 Aug;96(31):e7691

Department of Clinical Medicine, Medical College of Yan'an University, Yan'an, Shanxi, P. R. China.

This study is to investigate the expression of methyl CpG binding protein 2 (MECP2) in gastric cancer (GC) and its clinical significance.Expression of MECP2 was analyzed in 69 cases of GC tissues and 12 paracancerous tissues, either by qRT-PCR at the mRNA level or by Western blot and immunochemistry at the protein level. The correlation of MECP2 expression with clinicopathological parameters was analyzed in the 69 GC patients, and validated in data from the TCGA database. The effect of MECP2 expression on survival was also investigated.MECP2 was significantly increased at both mRNA and protein levels in GC compared with paracancerous tissues. MECP2 positive expression was significantly correlated with the TNM stages, histological types, and lymph node metastasis status, but was not correlated with sex or age. Significantly shorter overall survival and disease-free survival was observed in MECP2 positive GC cases compared with the MECP2 negative cases. Univariate and multivariate analyses showed that gender, histological type, lymph node metastasis, and MECP2 expression were independent prognostic factors of GC.The dysregulated expression of MECP2 in GC and its correlation to clinicopathological parameters indicate that MECP2 may regulate the development of GC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000007691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626154PMC
August 2017

Effects of oil quality and antioxidant supplementation on sow performance, milk composition and oxidative status in serum and placenta.

Lipids Health Dis 2017 Jun 7;16(1):107. Epub 2017 Jun 7.

Institute of Animal Nutrition, Sichuan Agricultural University, No. 46 Xinkang Road, Yaan, Sichuan, 625014, China.

Background: The aim of this study was to determine the effects of oil quality and antioxidant (AOX) supplementation on sow performance, milk composition and oxidative status.

Methods: A total of 80 PIC (PIC breeding, 3 ~ 5 parities) sows with similar body condition were allocated to four groups (n = 20), receiving diets including fresh corn oil, oxidized corn oil, fresh corn oil plus AOX and oxidized corn oil plus AOX, respectively, from d 85 of gestation to d 21 of lactation. AOX was provided at 200 mg/kg diet and mixed with corn oil prior to dietary formulation.

Results: The results showed that sows fed oxidized corn oil had significantly lower feed intake (P < 0.05) during lactation period. Feeding oxidized corn oil markedly decreased (P < 0.05) the contents of protein and fat in colostrums and milk, but the addition of AOX in oxidized corn oil prevented the decrease on protein content of colostrums. Moreover, sows fed oxidized corn oil had significantly lower serum activities of total SOD and Mn-SOD across lactation (P < 0.05). In contrast, addition of AOX to oxidized corn oil tended to inhibit the production of MDA (P = 0.08) in sows across lactation relative to fresh oil. Intriguingly, the placental oxidative status was affected by oil quality and AOX supplementation, as indicated by the markedly increased placental gene expression of GPX and SOD (P < 0.05) in sows fed oxidized corn oil but normalized by supplementation of AOX.

Conclusion: In conclusion, feeding oxidized corn oil did not markedly affect reproductive performance in addition to decreasing feed intake during lactation. Milk composition and systemic oxidative status were deteriorated in sows fed oxidized corn oil and partially improved by AOX supplementation. Moreover, placental antioxidant system of sows may have an adaptive response to oxidative stress, but normalized by AOX.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12944-017-0494-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5463408PMC
June 2017

[Study on effect of voltage-gated calcium channel protein in meridian tissue cells exciting conduction].

Zhongguo Zhen Jiu 2016 Oct;36(10):1051-1055

Henan University of CM, ZhengZhou 450046, China.

Objective: To explore the material basis of conduction along meridian.

Methods: Sixty SD rats(30 males,30 females) were randomly assigned into a normal group,an acupuncture group,a verapamil blocking group and a 0.9%NaCl blocking group(control group),15 rats in each one. Fluo 3-AM(calcium fluorescence probe) was injected at the observation part in femoral stomach meridian of foot-(meridian part) and the approaching femoral meridian part(non-meridian part) in the normal group and the acupuncture group,and then incubation was applied. In the verapamil blocking group,verapamil was injected at local meridian part and non-meridian part,and in the control group 0.9%NaCl was injected. Then Fluo 3-AM was injected at the meridian part and non-meridian part in the two groups,and incubation was implemented. Ca imaging changes in cells were recorded for more than 20 min after injection of every part in each group respectively. After the above operations in the last three groups,acupuncture was used at "Zusanli"(ST 36) immediately,with electroacupuncture for one min,then Ca imaging changes in cells at the meridian and non-meridian parts were recorded for more than 20 min.

Results: In the normal group, Ca fluorescence intensity at the meridian part was higher than that at the non-meridian part. In the acupuncture group,after acupuncture Ca fluorescence intensity at the meridian part was obviously higher than before,but the change before and after acupuncture was not apparent at the non-meridian part. After verapamil blocking local calcium channel and acupuncture,the Ca fluorescence of the meridian part did not strengthen,and the change of that before and after acupuncture at the non-meridian part was not obvious. In the control group,after injecting 0.9%NaCl at local part,Ca fluorescence intensities of the meridian and non-meridian parts showed no obvious change,so was that before and after acupuncture.

Conclusions: The voltage-gated calcium channel at the meridian part is highly correlated with its tissue cells exciting conduction.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.13703/j.0255-2930.2016.10.013DOI Listing
October 2016

Superior growth performance in broiler chicks fed chelated compared to inorganic zinc in presence of elevated dietary copper.

J Anim Sci Biotechnol 2016 29;7:13. Epub 2016 Feb 29.

Research and Development, Novus International, 20 Research Park Drive, Saint Charles, MO 63304 USA.

Background: The goal of this study was to compare the antagonism of elevated dietary Cu (250 mg/kg) from CuSO4 on three different Zn sources (ZnSO4 · H2O; [Zn bis(-2-hydroxy-4-(methylthio)butanoic acid)], Zn(HMTBa)2, a chelated Zn methionine hydroxy analogue; and Zn-Methionine), as measured using multiple indices of animal performance in ROSS 308 broilers.

Methods: Three experiments were conducted in broiler chicks fed a semi-purified diet. All birds were fed a Zn-deficient diet (8.5 mg/kg diet) for 1 wk, and then provided with the experimental diets for 2 wks.

Results: Experiment 1 was a 2 × 2 factorial design with two levels of Cu (8 vs. 250 mg/kg diet from CuSO4) and two Zn sources at 30 mg/kg [ZnSO4 · H2O vs. Zn(HMTBa)2]. Elevated Cu impaired growth performance only in birds fed ZnSO4. Compared to ZnSO4 · H2O, Zn(HMTBa)2 improved feed intake (12 %; P < 0.001) and weight gain (12 %, P < 0.001) and the benefits were more pronounced in the presence of 250 mg/kg diet Cu. Experiment 2 was a dose titration of ZnSO4 · H2O and Zn(HMTBa)2 at 30, 45, 60, and 75 mg/kg diet in the presence of 250 mg/kg CuSO4. Feed:gain was decreased and tibia Zn was increased with increasing Zn levels from 30 to 75 mg/kg. Birds fed Zn(HMTBa)2 consumed more food and gained more weight compared to birds fed ZnSO4, especially at lower supplementation levels (30 and 45 mg/kg; interaction P < 0,05). Experiment 3 compared two organic Zn sources (Zn(HMTBa)2 vs. Zn-Methionine) at 30 mg/kg with or without 250 mg/kg CuSO4. No interactions were observed between Zn sources and Cu levels on performance or tissue mineral concentrations. High dietary Cu decreased weight gain (P < 0.01). Tibia Cu and liver Cu were significantly increased with 250 mg/kg dietary Cu supplementation (P < 0.01). No difference was observed between the two Zn sources.

Conclusions: Dietary 250 mg/kg Cu significantly impaired feed intake and weight gain in birds fed ZnSO4 · H2O, but had less impact in birds fed Zn(HMTBa)2. No difference was observed between the two organic zinc sources. These results are consistent with the hypothesis that chelated organic Zn is better utilized than inorganic zinc in the presence of elevated Cu.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s40104-016-0072-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4772281PMC
March 2016

Superior Na-Storage Performance of Low-Temperature-Synthesized Na3(VO(1-x)PO4)2F(1+2x) (0≤x≤1) Nanoparticles for Na-Ion Batteries.

Angew Chem Int Ed Engl 2015 Aug 15;54(34):9911-6. Epub 2015 Jul 15.

Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 (USA).

Na-ion batteries are becoming comparable to Li-ion batteries because of their similar chemical characteristics and abundant sources of sodium. However, the materials production should be cost-effective in order to meet the demand for large-scale application. Here, a series of nanosized high-performance cathode materials, Na3(VO(1-x)PO4)2F(1+2x) (0≤x≤1), has been synthesized by a solvothermal low-temperature (60-120 °C) strategy without the use of organic ligands or surfactants. The as-synthesized Na3(VOPO4)2F nanoparticles show the best Na-storage performance reported so far in terms of both high rate capability (up to 10 C rate) and long cycle stability over 1200 cycles. To the best of our knowledge, the current developed synthetic strategy for Na3(VO(1-x)PO4)2F(1+2x) is by far one of the least expensive and energy-consuming methods, much superior to the conventional high-temperature solid-state method.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/anie.201503188DOI Listing
August 2015

Treatment of refractory/relapsed adult acute lymphoblastic leukemia with bortezomib-based chemotherapy.

Int J Gen Med 2015 12;8:211-4. Epub 2015 Jun 12.

Henan Key Lab of Experimental Haematology, Henan Institute of Haematology, Henan Tumor Hospital, Zhengzhou University, Zhengzhou, People's Republic of China.

Nine pretreated patients aged >19 years with relapsed/refractory acute lymphoblastic leukemia (ALL) were treated with a combination of bortezomib plus chemotherapy before allogeneic hematopoietic stem cell transplantation (allo-HSCT). Eight (88.9%) patients, including two Philadelphia chromosome-positive ALL patients, achieved a complete remission. Furthermore, the evaluable patients have benefited from allo-HSCT after response to this reinduction treatment. We conclude that bortezomib-based chemotherapy was highly effective for adults with refractory/relapsed ALL before allo-HSCT. Therefore, this regimen deserves a larger series within prospective trials to confirm these results.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IJGM.S59537DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4472074PMC
June 2015

Coexistence of P190 and P210 BCR/ABL transcripts in chronic myeloid leukemia blast crisis resistant to imatinib.

Springerplus 2015 9;4:170. Epub 2015 Apr 9.

Henan Key Lab of Experimental Haematology, Henan Institute of Haematology, Henan Tumor Hospital affiliated to Zhengzhou University, 127 Dongming Road, Zhengzhou, Henan Province 450008 China.

Introduction: Philadelphia chromosome (Ph) is a hallmark of chronic myeloid leukemia (CML), which exists in more than 90% CML and in 3% to 40% acute lymphoblastic leukemia (ALL).

Case Description: A 25-year-old man was diagnosed with CML in chronic phase. He first received treatment with hydroxyurea, achieving hematological remission and following imatinib mesylate for main treatment. A year later, he began to appear unexplained high fever with ineffective antibiotic treatment and bone morrow and blood tests indicated blast crisis. Both BCR/ABL 210 and BCR/ABL 190 fusion transcript were positive. Imatinib resistance was confirmed by a screening for ABL kinase domain E255K mutations, and dasatinib was administered. After two months, the patient went on to hematological remission.

Discussion And Evaluation: During medical treatment for CML, we experienced a relatively rare case with co-expression of the p210 and p190 encoding BCR-ABL transcripts in blastic phase. Imatinib resistance was confirmed and remission wasn't easily obtained, yet dasatinib was helpful. When resistance emerges, the treatment options include increasing the daily dose of imatinib, or combining imatinib with other agents. Of course, dasatinib, nilotinib and bone marrow transplantation are good choice as well.

Conclusions: The presence of p-190 transcript in CML may be related to progression of the disease. Thus monitoring the resistance of imatinib in CML patients, especially for advanced phase CML and BCR-ABL ALL, may be meaningful to guide clinical treatment and predict the prognosis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s40064-015-0930-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4397260PMC
April 2015

A phase-transfer assisted solvo-thermal strategy for low-temperature synthesis of Na3(VO1-xPO4)2F1+2x cathodes for sodium-ion batteries.

Chem Commun (Camb) 2015 Apr;51(33):7160-3

Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.

We demonstrate that a series of high-performance cathode materials, sodium vanadium polyanionic compounds, Na3(VO1-xPO4)2F1+2x (x = 0, 0.5 and 1), can be synthesized by a phase-transfer assisted solvo-thermal strategy at a rather low temperature (80-140 °C) in one simple step, exhibiting a high Na storage capacity of ca. 120 mA h g(-1) and excellent cycling performance. This study makes a significant step to extend this strategy to the synthesis of functional materials from simple binary to complex multicomponent compounds.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c5cc01504aDOI Listing
April 2015

A preliminary study of the effect of curcumin on the expression of p53 protein in a human multiple myeloma cell line.

Oncol Lett 2015 Apr 9;9(4):1719-1724. Epub 2015 Feb 9.

Department of Hematology, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan, P.R. China.

Curcumin is an inexpensive, natural plant ingredient with protease inhibitor effects. The present study aimed to analyze the inhibitory effects of curcumin on the multiple myeloma (MM) RPMI 8226 cell line, and examine the underlying mechanism that promotes the apoptosis of RPMI 8226 cells. A growth curve was constructed in order to observe the relative growth velocity, and MTT was used to analyze the effect of different concentrations of curcumin on inhibiting the proliferation of the RPMI 8226 cells. The mRNA expression of the , and genes was detected using quantitative polymerase chain reaction. The expression of p53 protein in the MM RPMI 8226 cells following treatment with curcumin was detected by western blotting and ELISA. Curcumin inhibited the proliferation of the MM RPMI 8226 cells in a dose- and time-dependent manner. In the MM RPMI 8226 cells treated with curcumin, the expression of the and genes was upregulated, while the expression of the gene was downregulated. p53 protein expression was higher in the curcumin experimental group compared with the control group. Subsequent to treatment with curcumin, the growth of the MM RPMI 8226 cell line was inhibited in a concentration- and time-dependent manner. In the MM RPMI 8226 cells treated with curcumin, p53 protein levels were upregulated, which suggested that curcumin may promote the apoptosis of MM cells by upregulating p53 protein expression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3892/ol.2015.2946DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4356419PMC
April 2015

A phase transfer assisted solvo-thermal strategy for the synthesis of REF₃ and Ln³⁺-doped REF3 nano-/microcrystals.

J Colloid Interface Sci 2014 Dec 16;436:171-8. Epub 2014 Sep 16.

Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.

Monodisperse orthorhombic-phase rare earth fluorides nano-/microcrystals with a special shape of disk-stacked cylinder have been synthesized via a facile phase transfer assisted solvo-thermal route, where an acid-base-coupled extractant has been employed to transfer hydrofluoric acid into an oil phase as a fluoride source. The synthetic parameters have been optimized and a possible formation mechanism has also been proposed. More importantly, the adopted acid-base-coupled extractant in this route can be recycled. Surveying all of the lanthanides from La to Lu, most of the heavy rare earths, such as Tb, Dy, Ho, Er, Tm and Yb, can form LnF3 nanocrystals with the similar morphologies. Furthermore, Ln(3+)-doped YF3 (Ln=Tb, Yb/Er) nanocrystals have also been synthesized, and their down-conversion and up-conversion (980 nm) luminescent properties were examined. The current approach could be extended to synthesize other metal fluorides nanoparticles.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2014.08.067DOI Listing
December 2014

Arsenic trioxide and microRNA-204 display contrary effects on regulating adipogenic and osteogenic differentiation of mesenchymal stem cells in aplastic anemia.

Acta Biochim Biophys Sin (Shanghai) 2014 Oct 3;46(10):885-93. Epub 2014 Sep 3.

Henan Key Lab of Experimental Haematology, Henan Institute of Haematology, Henan Tumor Hospital affiliated to Zhengzhou University, Zhengzhou 450008, China

Our previous studies have demonstrated that arsenic trioxide (ATO) had the clinical efficacy in treating patients with aplastic anemia (AA). However, the mechanisms remain to be elucidated. The important components of the bone marrow hematopoietic microenvironment, bone marrow mesenchymal stem cells (BMSCs), are often altered in AA patients. In this study, it was found that AA BMSCs were prone to be induced into adipocytes rather than osteoblasts. ATO treatment can at least partially restore the differentiation imbalance of AA BMSCs. We further identified miR-204 as a key regulator in AA BMSC differentiation. Luciferase reporter assay showed that miR-204 could directly bind to the 3'-untranslated region of Runx2 mRNA, a key transcription factor regulating osteogenesis. Moreover, adipogenic differentiation was promoted and osteogenic differentiation was inhibited in miR-204 over-expressed cells, whereas osteogenesis was enhanced and adipocyte formation was inhibited in cells that lost miR-204 function, which suggested its endogenous function. Together we showed that ATO could inhibit adipogenic differentiation, but promote osteogenic differentiation in AA BMSCs, providing a possible explanation for ATO clinical efficacy in AA patients. MiR-204 plays a key role in regulating BMSCs differentiation, and down-regulating miR-204 expression might be a novel strategy to treat AA.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/abbs/gmu082DOI Listing
October 2014

Intraocular pressure-lowering efficacy and safety of bimatoprost 0.03% therapy for primary open-angle glaucoma and ocular hypertension patients in China.

BMC Ophthalmol 2014 Feb 25;14:21. Epub 2014 Feb 25.

Eye & ENT Hospital, Shanghai Medical College, Fudan University, Fenyang Road 83, Shanghai, Xuhui District 200031, China.

Background: To report the clinical outcomes in Chinese patients with primary open-angle glaucoma and ocular hypertension treated with bimatoprost 0.03% therapy.

Methods: Two hundred sixty-three Chinese patients with primary open-angle glaucoma and ocular hypertension who needed initial or additional intraocular pressure (IOP) lowering were recruited in this prospective, open-label, multicenter clinical study and were treated with bimatoprost 0.03%. Patients received bimatoprost 0.03% as initial, replacement or adjunctive IOP-lowering therapy, and follow-up visits were performed at week 1, and month 1 and 3 of the bimatoprost treatment. The efficacy outcome measure was the post-treatment IOP level. The safety outcome measures included the rate of medication-related symptoms, physical signs, reported adverse events, and the level of conjunctival hyperemia.

Results: Among 240 patients who could be categorized by pre-existing therapies and the bimatoprost therapy regimen in the study, IOP values observed in all medication conditions showed significant IOP reduction at all study visits compared with baseline. At 3 months, 8.0 ± 3.7 mmHg (32.0%) reduction in IOP was observed in treatment-naive patients after bimatoprost monotherapy; in the patients previously on various therapy regimens, 1.9 ± 2.8 mmHg (9.5%) to 6.4 ± 6.1 mmHg (24.8%) additional IOP lowering was achieved after switching to bimatoprost monotherapy or bimatoprost combination therapy. The most common adverse event was conjunctival hyperemia, mainly of trace and mild intensity.

Conclusions: Our results show that bimatoprost 0.03% was effective in lowering IOP with favorable safety in Chinese primary open-angle glaucoma and ocular hypertension patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/1471-2415-14-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3943806PMC
February 2014

Size-controlled synthesis and morphology evolution of bismuth trifluoride nanocrystals via a novel solvent extraction route.

Nanoscale 2013 Jan 3;5(2):518-22. Epub 2012 Dec 3.

Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.

Monodisperse orthorhombic-phase BiF(3) nanocrystals with various shapes were prepared easily by a novel solvent extraction approach using an acid-base-coupled extractant. More importantly, the extractant used in this route can be recycled after synthesis. Our results show great promise for further extending the method to the preparation of other metal-fluoride nanoparticles.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/c2nr33212dDOI Listing
January 2013

Monodisperse iron phosphate nanospheres: preparation and application in energy storage.

ChemSusChem 2012 Aug 12;5(8):1495-500. Epub 2012 Jun 12.

Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.

An approach to synthesize monodisperse nanospheres with nanoporous structure through a solvent extraction route using an acid-base-coupled extractant has been developed. The nanospheres form through self-assembly and templating by reverse micelles in the organic solvent extraction systems. More importantly, the used extractant in this route can be recycled. The power of this approach is demonstrated by the synthesis of monodisperse iron phosphate nanospheres, exhibiting promising applications in energy storage. The synthetic parameters have been optimized. Based on this, a possible formation mechanism is also proposed. The synthetic procedure is relatively simple and could be extended to synthesize other water-insoluble inorganic metal salts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/cssc.201100844DOI Listing
August 2012

In vitro reduction of hexavalent chromium by cytoplasmic fractions of Pannonibacter phragmitetus LSSE-09 under aerobic and anaerobic conditions.

Appl Biochem Biotechnol 2012 Feb 13;166(4):933-41. Epub 2011 Dec 13.

Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, No.1 Bei Er Tiao, Zhong Guan Cun, Haidian District, Beijing 100190, China.

Hexavalent chromate reductase was characterized and was found to be localized in the cytoplasmic fraction of a chromium-resistant bacterium Pannonibacter phragmitetus LSSE-09. The Cr(VI) reductase activity of cell-free extract (S₁₂) was significantly improved by external electron donors, such as NADH, glucose, acetate, formate, citrate, pyruvate, and lactate. The reductase activity was optimal at pH 7.0 with NADH as the electron donor. The aerobic and anaerobic Cr(VI)-reduction enhanced by 0.1 mM NADH were respectively 3.5 and 3.4 times as high as that without adding NADH. The Cr(VI) reductase activity was inhibited by Mn²⁺, Cd²⁺, Fe³⁺, and Hg²⁺, whereas Cu²⁺ enhanced the chromate reductase activity by 29% aerobically and 33% anaerobically. The aerobic and anaerobic specific Michaelis-Menten constant K(m) of S₁₂ fraction was estimated to be 64.95 and 47.65 μmol L⁻¹, respectively. The soluble S₁₅₀ fractions showed similar activity to S₁₂ and could reduce 39.7% and 53.4% of Cr(VI) after 1 h of incubation aerobically and anaerobically while the periplasmic contents showed no obvious reduction activity, suggesting an effective enzymatic mechanism of Cr(VI) reduction in the cytoplasmic fractions of the bacterium. Results suggest that the enzymatic reduction of Cr(VI) could be useful for Cr(VI) detoxification in wastewater.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12010-011-9481-yDOI Listing
February 2012

Block copolymer micellization induced microphase mass transfer: partition of Pd(II), Pt(IV) and Rh(III) in three-liquid-phase systems of S201-EOPO-Na2SO4-H2O.

J Colloid Interface Sci 2011 Oct 14;362(1):228-34. Epub 2011 Jun 14.

Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.

Three-liquid-phase partitioning of Pd(II), Pt(IV) and Rh(III) in systems of S201(diisoamyl sulfide)/nonane-EOPO(polyethylene oxide-polypropylene oxide random block copolymer)-Na(2)SO(4)-H(2)O was investigated. Experimental results indicated that the selective enrichment of Pd(II), Pt(IV) and Rh(III) respectively into the S201 organic top phase, EOPO-based middle phase and Na(2)SO(4) bottom phase was achieved by control over the phase behavior of the three-liquid-phase systems (TLPS). The microphase mass transfer behavior of Pt(IV), Pd(II) and Rh(III) was closely related to the micellization of EOPO molecules. A suggested micro-mechanism model and a mass transfer model describe the micellization of EOPO molecules and the effect on mass transfer of platinum ions across the microphase interfaces. The salting-out induced continuous dehydration and ordered arrangement of the hydrophilic PEO segments in amphiphilic EOPO micelle, and these are the main driving forces for mass transfer of platinum metal ions onto the exposed activity sites of the dehydrated PEO segments. The differences in microphase interfacial structure of EOPO micelles are crucial for the efficient separation between Pt(IV), Pd(II) and Rh(III).
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcis.2011.06.009DOI Listing
October 2011
-->