Publications by authors named "Junjie Liu"

302 Publications

The SOX9-MMS22L Axis Promotes Oxaliplatin Resistance in Colorectal Cancer.

Front Mol Biosci 2021 27;8:646542. Epub 2021 May 27.

Department of Experimental Research, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning, China.

Background: Colorectal cancer (CRC) is estimated to be one of the most common cancers and the leading cause of cancer-related death worldwide. SOX9 is commonly overexpressed in CRC and participates in drug resistance. In addition, DNA damage repair confers resistance to anticancer drugs. However, the correlation between DNA damage repair and high SOX9 expression is still unclear. In this study, we aimed to investigate the function and the specific underlying mechanism of the SOX9-dependent DNA damage repair pathway in CRC.

Methods: The expression levels of SOX9 and MMS22L in CRC were examined by immunohistochemistry (IHC) and TCGA analysis. RNA sequencing was conducted in RKO SOX9-deficient cells and RKO shControl cells. Mechanistic studies were performed in CRC cells by modulating SOX9 and MMS22L expression, and we evaluated drug sensitivity and DNA damage repair signaling events. In addition, we investigated the effect of oxaliplatin in tumors with SOX9 overexpression and low expression of MMS22L .

Results: Our study showed that SOX9 has a higher expression level in CRC tissues than in normal tissues and predicts poor prognosis in CRC patients. Overexpression and knockdown of SOX9 were associated with the efficacy of oxaliplatin. In addition, SOX9 activity was enriched in the DNA damage repair pathway regulation of MMS22L expression and participation in DNA double-strand break repair. SOX9 was upregulated and formed a complex with MMS22L, which promoted the nuclear translocation of MMS22L upon oxaliplatin treatment. Moreover, the enograft assay results showed that oxaliplatin abrogated tumor growth from cells with MMS22L downregulation in mice.

Conclusions: In CRC, activation of the SOX9-MMS22L-dependent DNA damage pathway is a core pathway regulating oxaliplatin sensitivity. Targeting this pathway in oxaliplatin-resistant CRC cells is a promising therapeutic option.
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http://dx.doi.org/10.3389/fmolb.2021.646542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191464PMC
May 2021

Role of ultramicropores in the remarkable gas storage in hypercrosslinked polystyrene networks studied by positron annihilation.

Phys Chem Chem Phys 2021 Jun 11. Epub 2021 Jun 11.

Hubei Nuclear Solid Physics Key Laboratory, Department of Physics, Wuhan University, Wuhan 430072, China.

In this paper, hypercrosslinked polystyrene (HCLPS) networks were synthesized by radical bulk polymerization and Friedel-Crafts alkylation reactions using vinylbenzyl-co-divinylbenzene chloride (VBC-DVB) as the precursors. A series of HCLPS was prepared with varying content of DVB from 0 to 10% in the precursor. Both N2 adsorption and positron annihilation measurements reveal micropores in the HCLPS. Especially, the existence of ultramicropores with a size in the range of 0.63-0.7 nm is confirmed by positron lifetime measurements. With increasing DVB content from 0 to 10%, the number of ultramicropores shows a gradual increase. Both the H2 and CO2 adsorption capacity increase monotonously with the increase of the DVB content. With 10% DVB in the HCLPS, the H2 storage increases to 10.3 mmol g-1 (2.05 wt%) at 77 K and 1 bar and the CO2 capture reaches 2.81 mmol g-1 (12.4 wt%) at 273 K and 1 bar. The remarkable gas storage ability is ascribed to the existence of the ultramicropores, which result in a stronger affinity to the gas molecules. By using positrons as a new probe for the pores, our results provide convincing evidence of the role of ultramicropores in the gas adsorption performance in microporous organic polymers.
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http://dx.doi.org/10.1039/d1cp01867aDOI Listing
June 2021

Feasibility of using tissue autolysis to estimate the postmortem interval in horses.

J Vet Diagn Invest 2021 Jun 10:10406387211021865. Epub 2021 Jun 10.

Comparative, Diagnostic, and Population Medicine, University of Florida, Gainesville, FL, USA.

Estimation of the postmortem interval (PMI) is a poorly studied field in veterinary pathology. The development of field-applicable methods is needed given that animal cruelty investigations are increasing continually. We evaluated various histologic criteria in equine brain, liver, and muscle tissue to aid the estimation of PMI in horses, which is central to forensic investigations of suspicious death. After death, autolysis proceeds predictably, depending on environmental conditions. Currently, no field-applied methods exist that accurately estimate the PMI using histology in animals or humans through quantification of autolysis. Brain, liver, and skeletal muscle from 12 freshly euthanized horses were held at 22°C and 8°C for 72 h. Tissues were sampled at T0h, T1h, T2h, T4h, T6h, T12h, T24h, T36h, T48h, T60h, and T72h. For each tissue, we quantified 5 to 7 criteria associated with autolysis, based on the percentage of microscopic field involved. Each criterion was modeled, with temperature and time as independent variables. Changes were most predictable in liver and muscle over the first 72 h postmortem. The criteria for autolysis that were present most extensively at both temperatures were hepatocyte individualization and the separation of bile duct epithelium from the basement membrane. The changes that were present next most extensively were disruption of myofiber continuity, hypereosinophilia, and loss of striation. Brain changes were highly variable. The high statistical correlation between the parameter "autolysis" and the variables "time/temperature", indicates that autolysis is progressive and predictable. Further investigation of these criteria is needed to establish histologic algorithms for PMI.
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http://dx.doi.org/10.1177/10406387211021865DOI Listing
June 2021

Boosting 5-ALA-based photodynamic therapy by a liposomal nanomedicine through intracellular iron ion regulation.

Acta Pharm Sin B 2021 May 29;11(5):1329-1340. Epub 2021 Apr 29.

Henan Key Laboratory of Targeting Therapy and Diagnosis for Critical Diseases, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.

5-Aminolevulinic acid (5-ALA) has been approved for clinical photodynamic therapy (PDT) due to its negligible photosensitive toxicity. However, the curative effect of 5-ALA is restricted by intracellular biotransformation inactivation of 5-ALA and potential DNA repair of tumor cells. Inspired by the crucial function of iron ions in 5-ALA transformation and DNA repair, a liposomal nanomedicine ([email protected]/DFO) with intracellular iron ion regulation property was developed for boosting the PDT of 5-ALA, which was prepared by co-encapsulating 5-ALA and DFO (deferoxamine, a special iron chelator) into the membrane fusion liposomes (MFLs). [email protected]/DFO showed an improved pharmaceutical behavior and rapidly fused with tumor cell membrane for 5-ALA and DFO co-delivery. [email protected]/DFO could efficiently reduce iron ion, thus blocking the biotransformation of photosensitive protoporphyrin IX (PpIX) to heme, realizing significant accumulation of photosensitivity. Meanwhile, the activity of DNA repair enzyme was also inhibited with the reduction of iron ion, resulting in the aggravated DNA damage in tumor cells. Our findings showed [email protected]/DFO had potential to be applied for enhanced PDT of 5-ALA.
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http://dx.doi.org/10.1016/j.apsb.2021.03.017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8148057PMC
May 2021

Construction of a ceRNA Network and a Prognostic lncRNA Signature associated with Vascular Invasion in Hepatocellular Carcinoma based on Weighted Gene Co-Expression Network Analysis.

J Cancer 2021 5;12(13):3754-3768. Epub 2021 May 5.

Hepatic Surgery Center, Tongji Medical College, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China.

Understanding risk factors for vascular invasion (VI) is crucial for assessing the risk of recurrence and overall prognosis of hepatocellular carcinoma (HCC). This study aimed to construct a prognostic long non-coding RNA (lncRNA) signature and a ceRNA Network associated with vascular invasion in HCC. Differentially expressed genes (DEGs) of HCC patients associated with VI were identified by analyzing data from TCGA. Weighted gene co-expression network analysis (WGCNA) was used to identify associations between gene expression modules and clinical features. A VI-related prognostic lncRNA signature was then established using univariate, LASSO and multivariate Cox proportional hazards regression analyses. Based on the hub modules identified by the WGCNA, we constructed a VI-related lncRNA-miRNA-mRNA ceRNA network and screened hub lncRNAs for further research. Finally, we conducted and experiments to determine the biological roles of the identified hub gene BBOX1-AS1. The key module related to VI and OS was identified using WGCNA, after which a prognostic model consisting of eight lncRNAs was established, and verified using time-dependent receiver operating characteristic (ROC) curve analysis. BBOX1-AS1 was confirmed to be highly expressed in HCC tissues, and its expression was significantly correlated with a poor prognosis. Silencing BBOX1-AS1 significantly suppressed the proliferation, migration and invasion of HCC cells. experiments demonstrated that knocking down of BBOX1-AS1 could result in significant decrease of tumor volume and tumor weight. The VI-related lncRNA signature established in this study can be used to predict the clinical outcomes of HCC patients. In addition, we constructed a VI-related lncRNA-miRNA-mRNA ceRNA network and demonstrated that BBOX1-AS1 might be a novel biomarker associated with VI in HCC.
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http://dx.doi.org/10.7150/jca.57260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176257PMC
May 2021

Sensitive Detection of Sulfide Ion Based on Fluorescent Ionic Liquid-Graphene Quantum Dots Nanocomposite.

Front Chem 2021 30;9:658045. Epub 2021 Apr 30.

Guangxi Medical University Cancer Hospital, Guangxi Medical University, Nanning, China.

Sulfide ions (S) that are widely distributed in biological and industrial fields are extremely toxic and pose great harms to both ecological environment and human health. However, fluorescent sensors toward S ions commonly use S-recovered fluorescence of fluorophore that is first quenched mainly by metal ions. Fluorescent probe which enables direct, selective, and sensitive detection of S ion is highly desirable. Herein, we demonstrate one-step preparation of fluorescent ionic liquid-graphene quantum dots (IL-GQDs) nanocomposite, which can act as a fluorescent probe for direct and sensitive detection of S ion. The IL-GQDs nanocomposite is easily synthesized facile molecular fusion of carbon precursor and surface modification of GQDs by IL under hydrothermal condition. The as-prepared IL-GQDs nanocomposite has uniform and ultrasmall size, high crystallinity, and bright green fluorescence (absolute photoluminescence quantum yield of 18.2%). S ions can strongly and selectively quench the fluorescence of IL-GQDs because of the anion exchange ability of IL. With IL-GQDs nanocomposite being fluorescent probe, direct and sensitive detection of S is realized with a linear detection range of 100nM-10μM and 10μM-0.2mM (limit of detection or LOD of 23nM). Detection of S ions in environmental river water is also achieved.
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http://dx.doi.org/10.3389/fchem.2021.658045DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120006PMC
April 2021

Hypoxic postconditioning-induced neuroprotection increases neuronal autophagy via activation of the SIRT1/FoxO1 signaling pathway in rats with global cerebral ischemia.

Exp Ther Med 2021 Jul 2;22(1):695. Epub 2021 May 2.

College of Nursing and Rehabilitation, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China.

Hypoxic postconditioning (HPC) has been reported to be a beneficial and promising treatment for global cerebral ischemia (GCI). However, its neuroprotective mechanism remains unclear. The aim of the present study was to determine whether the protective effects of HPC in a rat model of GCI were due to the upregulation of autophagy via the silent information regulator transcript-1 (SIRT1)/Forkhead box protein 1 (FoxO1) pathway. Morris water maze test revealed that HPC attenuated cognitive damage in GCI rats. HPC also significantly increased the levels of the autophagy-related protein LC3-II, SIRT1 and FoxO1 compared with those in the GCI group. However, the HPC-induced LC3-II upregulation was blocked by the SIRT1 inhibitor EX527. These results suggested that the beneficial effects of HPC on GCI rats were due to the upregulation of ischemiainduced autophagy and involved the SIRT1/FoxO1 signaling pathway.
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http://dx.doi.org/10.3892/etm.2021.10127DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111876PMC
July 2021

Extracellular vesicles in Inter-Kingdom communication in gastrointestinal cancer.

Am J Cancer Res 2021 15;11(4):1087-1103. Epub 2021 Apr 15.

Department of Gastrointestinal Surgery, Division of Colorectal & Anal Surgery Guangxi Medical University Cancer Hospital Nanning 530021, Guangxi Zhuang Autonomous Region, P. R. China.

The production and secretion of extracellular vesicles (EVs) are common features of cells (including various normal cells, neoplastic cell lines as well as bacteria) that span all domains of life. Tumor-derived exosomes are enriched with kinds of tumorigenesis mediators which are derived from the cytoplasm of cancer cells and fully reflect the tumor conditions. Indeed, the major topics and challenges on current oncological research are the identification of tumorigenic and metastatic molecules in tumor-cell-derived exosomes as well as elucidating the pathways that guarantee these components to be included in exosomes. The bacterial EVs have also been implicated in the pathogenesis of gastrointestinal (GI) tumors and chronic inflammatory diseases; however, the possible function of outer membrane vesicles (OMVs) in tumorigenesis remains largely underestimated. We suggest that EVs from both eukaryotic cells and different microbes in GI tract act as regulators of intracellular and cross-species communication, thus particularly facilitate tumor cell survival and multi-drug resistance. Therefore, our review introduces comprehensive knowledge on the promising role of EVs (mainly exosomes and OMVs) production of GI cancer development and gut microbiome, as well as its roles in developing novel therapeutic strategies.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085842PMC
April 2021

NOX1/NADPH oxidase is involved in the LPS-induced exacerbation of collagen-induced arthritis.

J Pharmacol Sci 2021 Jun 29;146(2):88-97. Epub 2021 Jan 29.

Department of Pharmacology, Kyoto Prefectural University of Medicine, Kyoto, Japan.

We investigate as yet an unidentified role of NOX1, a non-phagocytic isoform of the superoxide-generating NADPH oxidase, in immune responses using Nox1-knockout mice (Nox1-KO). The transcripts of NOX1 was expressed in lymphoid tissues, including the spleen, thymus, bone marrow, and inguinal lymphoid nodes. When antibody production after ovalbumin (OVA) immunization was examined, no significant differences were observed in serum anti-OVA IgG levels between wild-type mice (WT) and Nox1-KO. In the experimental asthma, the infiltration of eosinophils and the Th2 cytokine response after the induction of asthma with OVA were similar between the two genotypes. However, the severity and incidence of experimental collagen-induced arthritis (CIA) following the administration of a low dose of endotoxin (LPS) were significantly lower in Nox1-KO. While neither serum levels of autoantibodies nor in vitro cytokine responses were affected by Nox1 deficiency, NOX1 mRNA levels in the spleen significantly increased after the LPS challenge. Among the spleen cells, remarkable LPS-induced upregulation of NOX1 was demonstrated in both CD11b monocytes/macrophages and CD11c dendritic cells, suggesting that LPS-inducible NOX1 in monocytes/macrophages/dendritic cells may modulate the development of experimental CIA. Therapeutic targeting of NOX1 may therefore control the onset and/or severity of arthritis which is exacerbated by bacterial infection.
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http://dx.doi.org/10.1016/j.jphs.2021.01.009DOI Listing
June 2021

Genome-Wide Identification and Expression Patterns of the C2H2-Zinc Finger Gene Family Related to Stress Responses and Catechins Accumulation in [L.] O. Kuntze.

Int J Mol Sci 2021 Apr 18;22(8). Epub 2021 Apr 18.

The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, China.

The C2H2-zinc finger protein (C2H2-ZFP) is essential for the regulation of plant development and widely responsive to diverse stresses including drought, cold and salt stress, further affecting the late flavonoid accumulation in higher plants. Tea is known as a popular beverage worldwide and its quality is greatly dependent on the physiological status and growing environment of the tea plant. To date, the understanding of C2H2-ZFP gene family in [L.] O. Kuntze is not yet available. In the present study, 134 Cs genes were identified and randomly distributed on 15 chromosomes. The CsC2H2-ZFP gene family was classified into four clades and gene structures and motif compositions of CsC2H2-ZFPs were similar within the same clade. Segmental duplication and negative selection were the main forces driving the expansion of the CsC2H2-ZFP gene family. Expression patterns suggested that were responsive to different stresses including drought, salt, cold and methyl jasmonate (MeJA) treatment. Specially, several showed a significant correlation with the catechins content and responded to the MeJA treatment, which might contribute to the tea quality and specialized astringent taste. This study will lay the foundations for further research of C2H2-type zinc finger proteins on the stress responses and quality-related metabolites accumulation in .
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http://dx.doi.org/10.3390/ijms22084197DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8074030PMC
April 2021

Zone-Folded Longitudinal Acoustic Phonons Driving Self-Trapped State Emission in Colloidal CdSe Nanoplatelet Superlattices.

Nano Lett 2021 May 29;21(10):4137-4144. Epub 2021 Apr 29.

CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, P.R. China.

Colloidal CdSe nanoplatelets (NPLs) have substantial potential in light-emitting applications because of their quantum-well-like characteristics. The self-trapped state (STS), originating from strong electron-phonon coupling (EPC), is promising in white light luminance because of its broadband emission. However, achieving STS in CdSe NPLs is extremely challenging because of their intrinsic weak EPC nature. Herein, we developed a strong STS emission in the spectral range of 450-600 nm by building superlattice (SL) structures with colloidal CdSe NPLs. We demonstrated that STS is generated via strong coupling of excitons and zone-folded longitudinal acoustic phonons with formation time of ∼450 fs and localization length of ∼0.56 nm. The Huang-Rhys factor, describing the EPC strength in SL structure, is estimated to be ∼19.9, which is much larger than that (∼0.1) of monodispersed CdSe NPLs. Our results provide an in-depth understanding of STS and a platform for generating and manipulating STS by designing SL structures.
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http://dx.doi.org/10.1021/acs.nanolett.0c04169DOI Listing
May 2021

Hierarchically porous calcium-silicon nanosphere-enabled co-delivery of microRNA-210 and simvastatin for bone regeneration.

J Mater Chem B 2021 04;9(16):3573-3583

School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, P. R. China. and National Engineering Research Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, P. R. China. and Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou 510006, P. R. China.

The regenerative repair of large bone defects is a major problem in orthopedics and clinical medicine. The key problem is the lack of ability of existing bone graft materials to promote osteogenesis and angiogenesis. Previous studies have shown that the osteogenic or angiogenic abilities of these materials could be significantly improved by adding miRNA or small-molecule drugs to bone graft materials; however, the synergistic effect arising from this combination is not clear. Therefore, we proposed to construct a dual drug delivery system that could simultaneously achieve the co-encapsulation and co-delivery of miRNA and small-molecule drugs to explore the effect of a dual drug delivery system on bone repair. In this study, we constructed dual-sized pore structure calcium-silicon nanospheres (DPNPs) and achieved the co-encapsulation of miR-210, angiogenic gene drugs, and simvastatin (Siv), a small-molecule osteogenic drug, through metal-ion coordination and physical adsorption. In vitro and in vivo osteogenic and angiogenic experiments showed that the dual drug delivery system (Siv/DPNP/miR-210) exhibited better properties than those of the individual unloaded and single drug-loaded systems and could significantly accelerate the process of bone repair, which provides a novel strategy for the regeneration and repair of bone defects.
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http://dx.doi.org/10.1039/d1tb00063bDOI Listing
April 2021

Cabin air quality on non-smoking commercial flights: A review of published data on airborne pollutants.

Indoor Air 2021 Apr 24. Epub 2021 Apr 24.

International Centre for Indoor Environment and Energy, Department of Civil Engineering, Technical University of Denmark, Lyngby, Denmark.

We reviewed 47 documents published 1967-2019 that reported measurements of volatile organic compounds (VOCs) on commercial aircraft. We compared the measurements with the air quality standards and guidelines for aircraft cabins and in some cases buildings. Average levels of VOCs for which limits exist were lower than the permissible levels except for benzene with average concentration at 5.9 ± 5.5 μg/m . Toluene, benzene, ethylbenzene, formaldehyde, acetaldehyde, limonene, nonanal, hexanal, decanal, octanal, acetic acid, acetone, ethanol, butanal, acrolein, isoprene and menthol were the most frequently measured compounds. The concentrations of semi-volatile organic compounds (SVOCs) and other contaminants did not exceed standards and guidelines in buildings except for the average NO concentration at 12 ppb. Although the focus was on VOCs, we also retrieved the data on other parameters characterizing cabin environment. Ozone concentration averaged 38 ppb below the upper limit recommended for aircraft. The outdoor air supply rate ranged from 1.7 to 39.5 L/s per person and averaged 6.0 ± 0.8 L/s/p (median 5.8 L/s/p), higher than the minimum level recommended for commercial aircraft. Carbon dioxide concentration averaged 1315 ± 232 ppm, lower than what is permitted in aircraft and close to what is permitted in buildings. Measured temperatures averaged 23.5 ± 0.8°C and were generally within the ranges recommended for avoiding thermal discomfort. Relative humidity averaged 16% ± 5%, lower than what is recommended in buildings.
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http://dx.doi.org/10.1111/ina.12831DOI Listing
April 2021

Coherences and the thermodynamic uncertainty relation: Insights from quantum absorption refrigerators.

Phys Rev E 2021 Mar;103(3-1):032138

Department of Chemistry and Centre for Quantum Information and Quantum Control, University of Toronto, 80 Saint George Street, Toronto, Ontario, M5S 3H6, Canada.

The thermodynamic uncertainty relation, originally derived for classical Markov-jump processes, provides a tradeoff relation between precision and dissipation, deepening our understanding of the performance of quantum thermal machines. Here, we examine the interplay of quantum system coherences and heat current fluctuations on the validity of the thermodynamics uncertainty relation in the quantum regime. To achieve the current statistics, we perform a full counting statistics simulation of the Redfield quantum master equation. We focus on steady-state quantum absorption refrigerators where nonzero coherence between eigenstates can either suppress or enhance the cooling power, compared with the incoherent limit. In either scenario, we find enhanced relative noise of the cooling power (standard deviation of the power over the mean) in the presence of system coherence, thereby corroborating the thermodynamic uncertainty relation. Our results indicate that fluctuations necessitate consideration when assessing the performance of quantum coherent thermal machines.
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http://dx.doi.org/10.1103/PhysRevE.103.032138DOI Listing
March 2021

Structure-Gas Barrier Property Relationship in a Novel Polyimide Containing Naphthalene and Amide Groups: Evaluation by Experiments and Simulations.

Materials (Basel) 2021 Mar 13;14(6). Epub 2021 Mar 13.

State Key Laboratory for Powder Metallurgy, Centre South University, Changsha 410083, China.

In order to meet the increasingly stringent requirements for heat resistance and barrier properties in the packaging and electronic device encapsulation field. A high-barrier polyimide (NAPPI) contains naphthalene ring and amide group was prepared by polymerization of a novel diamine (NAPDA) and pyromellitic dianhydride. The structure and properties of diamine monomers and polymers were characterized. Results show that the NAPPI exhibits superior barrier properties with extremely low water vapor and oxygen transmission rate values of 0.14 g·m·day and 0.04 cm·m·day, respectively. In addition, the NAPPI presents outstanding mechanical properties and thermal stability as well. This article attempts to explore the relationship between NAPPI structure and barrier properties by combining experiment and simulation. Studies on positron annihilation lifetime spectroscopy, Wide angle X-ray diffractograms and molecular dynamics simulations prove that the NAPPI has smaller interplanar spacing and higher chain regularity. In addition, the strong chain rigidity and interchain cohesion of NAPPI due to the presence of the rigid naphthalene ring and a large number of hydrogen bond interactions formed by amide groups result in compact chain packing and smaller free volume, which reduces the solubility and diffusibility of small molecules in the matrix. In general, the simulation results are consistent with the experimental results, which are important for understanding the barrier mechanism of NAPPI.
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http://dx.doi.org/10.3390/ma14061402DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7999945PMC
March 2021

Complete Genome Sequence of sp. Strain IGA-FME-1, Isolated from the Bulk Soil of Maize ( L.).

Microbiol Resour Announc 2021 Apr 1;10(13). Epub 2021 Apr 1.

Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, China

Here, we present the complete genome of sp. strain IGA-FME-1 (isolated from the bulk soil of maize [ L.]). This genome consists of 5,147,837 bp, 5,219 protein-coding genes, 112 tRNAs, 13 16S rRNAs, 13 23S rRNAs, and 13 5S rRNAs, with a G+C content of 38.2%.
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http://dx.doi.org/10.1128/MRA.00192-21DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8104053PMC
April 2021

Coherent electric field manipulation of Fe spins in PbTiO.

Sci Adv 2021 Mar 3;7(10). Epub 2021 Mar 3.

Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Magnetoelectrics, materials that exhibit coupling between magnetic and electric degrees of freedom, not only offer a rich environment for studying the fundamental materials physics of spin-charge coupling but also present opportunities for future information technology paradigms. We present results of electric field manipulation of spins in a ferroelectric medium using dilute ferric ion-doped lead titanate as a model system. Combining first-principles calculations and electron paramagnetic resonance (EPR), we show that the ferric ion spins are preferentially aligned perpendicular to the ferroelectric polar axis, which we can manipulate using an electric field. We also demonstrate coherent control of the phase of spin superpositions by applying electric field pulses during time-resolved EPR measurements. Our results suggest a new pathway toward the manipulation of spins for quantum and classical spintronics.
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http://dx.doi.org/10.1126/sciadv.abf8103DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7929503PMC
March 2021

Electrochemiluminescence Based on a Dual Carbon Ultramicroelectrode with Confined Steady-State Annihilation.

Anal Chem 2021 Mar 3;93(10):4528-4535. Epub 2021 Mar 3.

Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China.

Developing novel microelectronic devices for electrochemical measurements and electrochemiluminescence (ECL) study is of great importance. Herein, we fabricated a submicrometer-sized dual carbon electrode (DCE) and investigated its annihilation ECL behavior under steady-state conditions for the first time. The oxidation and reduction of the model luminophore, [Ru(bpy)], occurred separately at the two sides of the DCE, and the electrogenerated ions then diffused to the gap between the two electrodes to generate the excited-state intermediate [Ru(bpy)] and ECL emission. Compared with other types of two-electrode systems, the prepared DCE possesses a smaller total size and an ultrasmall interelectrode distance of 60 nm or less, which could result in a shorter diffusion time and an amplified ECL signal without the purification of the solvent and supporting electrolytes. On the basis of the constructed ECL microscopic platform, we successfully obtained a stable and confined ECL signal in the vicinity of the electrode tip. Furthermore, a two-dimensional finite element method simulation of this model system was performed to quantitively analyze the concentration profiles of the electrogenerated species around the tip of the DCE and predict the concentrations of [Ru(bpy)] with various gap distances. The simulation results also proved that the higher concentrations of [Ru(bpy)] could be achieved with a smaller distance with a possible amplification factor of 6 (compared with the concentration when the gap distance is greater than 300 nm). This work provides an experimental model for further improvement of ECL efficiency and broadens the availability for annihilation ECL applications in small confined spaces.
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http://dx.doi.org/10.1021/acs.analchem.0c04954DOI Listing
March 2021

MicroRNAs Expression Patterns Predict Tumor Mutational Burden in Colorectal Cancer.

Front Oncol 2020 9;10:550986. Epub 2021 Feb 9.

Department of Gastrointestinal Surgery, Affiliated Tumor Hospital, Guangxi Medical University, Nanning, China.

Background: Tumor mutational burden (TMB) could be a measure of response to immune checkpoint inhibitors therapy for patients with colorectal cancer (CRC). MicroRNAs (miRNAs) participate in anticancer immune responses. In the present study, we determined miRNA expression patterns in patients with CRC and built a signature that predicts TMB.

Methods: Next generation sequencing (NGS) on formalin-fixed paraffin-embedded samples from CRC patients was performed to measure TMB levels. We used datasets from The Cancer Genome Atlas to compare miRNA expression patterns in samples with high and low TMB from patients with CRC. We created an miRNA-based signature index using the selection operator (LASSO) and least absolute shrinkage method from the training set. We used an independent test set as internal validation. We used real-time polymerase chain reaction (RT-PCR) to validate the miRNA-based signature classifier.

Results: Twenty-seven samples from CRC patients underwent NGS to determine the TMB level. We identified four miRNA candidates in the training set for predicting TMB (N = 311). We used the test set (N = 204) for internal validation. The four-miRNA-based signature classifier was an accurate predictor of TMB, with accuracy 0.963 in the training set. In the test set, it was 0.902; and it was 0.946 in the total set. The classifier was superior to microsatellite instability (MSI) for predicting TMB in TCGA dataset. In the validation cohort, MSI status more positively correlated with TMB levels than did the classifier. Validation from RT-qPCR showed good target discrimination of the classifier for TMB prediction.

Conclusion: To our knowledge, this is the first miRNA-based signature classifier validated using high quality clinical data to accurately predict TMB level in patients with CRC.
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http://dx.doi.org/10.3389/fonc.2020.550986DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7900489PMC
February 2021

Widely Targeted Metabolomics Analysis Reveals Key Quality-Related Metabolites in Kernels of Sweet Corn.

Int J Genomics 2021 8;2021:2654546. Epub 2021 Feb 8.

Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, 510642 Guangdong, China.

Sweet corn ( var. ) is a major economic vegetable crop. Different sweet corn cultivars vary largely in flavor, texture, and nutrition. The present study performed widely targeted metabolomics analysis based on the HPLC-MS/MS technology to analyze the metabolic profiles in three sweet corn cultivars widely grown in China. A total of 568 metabolites in the three sweet corn cultivars were detected, of which 262 differential metabolites significantly changed among cultivars. Carbohydrates, organic acids, and amino acids were the majority detected primary metabolites. Organic acids were mainly concentrated on shikimate, benzoic acids, and quinic acid with aromatic groups. And the essential amino acids for the human body, methionine and threonine, were highly accumulated in the high-quality cultivar. In addition, phenylpropanoids and alkaloids were the most enriched secondary metabolites while terpenes were low-detected in sweet corn kernels. We found that the flavonoids exist in both free form and glycosylated form in sweet corn kernels. PCA and HCA revealed clear separations among the three sweet corn cultivars, suggesting distinctive metabolome profiles among three cultivars. The differential metabolites were mapped into flavonoid biosynthesis, phenylpropanoid biosynthesis, biosynthesis of amino acids, and other pathways according to the KEGG classification. Furthermore, we identified skimmin, N',N-diferuloylspermidine, and 3-hydroxyanthranilic acid as the key quality-related metabolites related to grain quality traits in sweet corn. The results suggested variations of metabolic composition among the three cultivars, providing the reference quality-related metabolites for sweet corn breeding.
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http://dx.doi.org/10.1155/2021/2654546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884158PMC
February 2021

Associations of indoor carbon dioxide concentrations, air temperature, and humidity with perceived air quality and sick building syndrome symptoms in Chinese homes.

Indoor Air 2021 Feb 23. Epub 2021 Feb 23.

School of Mechanical Engineering, Purdue University, West Lafayette, IN, USA.

The indoor environment influences occupants' health. From March 1, 2018, to February 28, 2019, we continuously monitored indoor temperature (T), relative humidity (RH), and CO concentration in bedrooms via an online system in 165 residences that covered all five climate zones of China. Meanwhile, we asked one specific occupant in each home to complete questionnaires about perceived air quality and sick building syndrome (SBS) symptoms at the end of each month. Higher CO concentration was significantly associated with a higher percentage of perceived stuffy odor and skin SBS symptoms. Higher relative humidity was associated with higher percentage of perceived moldy odor and humid air, while lower RH was associated with a higher percentage of perceived dry air. Occupants who lived in residences with high RH were less likely to have mucosal and skin SBS symptoms (adjusted odds ratio (AOR): 0.73-0.78). However, the benefit of high humidity for perceived dry air and skin dryness symptoms is weaker if there is a high CO concentration level.
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http://dx.doi.org/10.1111/ina.12810DOI Listing
February 2021

Analysis of the screening results of 24040 potential sperm donors in a human sperm bank in Henan Province, China: a 14-year retrospective cohort study.

Hum Reprod 2021 04;36(5):1205-1212

Center for Reproductive Medicine, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

Study Question: Is there a relation between the characteristics of potential sperm donors and the acceptance rate of these potential donors?

Summary Answer: A relatively higher acceptance rate was observed for potential sperm donors who were aged ≤ 35 years, were married, had children, and who had received higher education, and acceptance rates were also higher during spring and winter than summer and autumn.

What Is Known Already: Recruiting donors to a sperm bank program is difficult and slow owing to the high rates of rejection and dropout.

Study Design, Size, Duration: A total of 24040 potential sperm donors were screened by the Henan Human Sperm Bank from 2006 to 2019.

Participants/materials, Setting, Methods: Potential sperm donors were recruited using the following baseline requirement: height of 168 cm or taller; age 22-45 years; currently attending or had graduated from high school or above. Men who met the criteria for age, height, and education level were invited for semen quality screening. The acceptable criteria for semen samples included liquefaction time < 60min, volume ≥ 2mL, sperm concentration ≥ 60 × 106/mL, progressive motility ≥ 60%, post-thaw motility ≥ 40%, pre-freezing total motile sperm per vial > 30 × 106/mL, post-thaw total motile sperm per vial > 12 × 106/mL, and freeze-thaw survival rate ≥ 60%. Any potential sperm donors meeting the minimum criteria for acceptable semen quality on two consecutive semen samples were scheduled for clinical assessment, physical examination, and laboratory tests. The reasons for sperm donor rejection were analyzed. The characteristics of accepted and rejected donors were compared using the chi-square test, and multivariate logistic regression analyses were conducted to identify factors associated with the acceptance rate of potential sperm donors and the positive rate of sexually transmitted diseases (STDs).

Main Results And The Role Of Chance: Only 23.38% (5620/24040) of potential sperm donors were accepted. The top four reasons for rejection were suboptimal semen quality (90.27%), STDs (6.26%), dropped out (2.65%), and chromosomal abnormalities (0.35%). The most common reason for the rejection of donors with an STD was a positive test for mycoplasmas (49.05%), followed by hepatitis B virus (27.56%), Chlamydia trachomatis (4.68%), and Escherichia coli (3.03%). n this study, the acceptance rate for men aged ≤ 35 years was significantly higher than that for men aged >35 years (P < 0.05). The acceptance rates were also significantly higher for men with a higher education than for men with lower education, married men than unmarried men, and men with children than men without children (P < 0.05). Moreover, acceptance rates were significantly higher during spring and winter than during summer (P <0.05) but were not significantly higher during autumn than during summer (P >0.05).

Limitations, Reasons For Caution: This study was not performed to analyze the effect of lifestyle habits, such as alcohol consumption and cigarette smoking, on the acceptance rate of potential sperm donors.

Wider Implications Of The Findings: Only a small proportion of potential sperm donors were accepted in this anonymous sperm donor program. New strategies for sperm donor recruitment may be required to improve the acceptance rate. In the future, we may have to target potential sperm donors who are aged ≤ 35 years and who received higher education in order to improve the acceptance rate.

Study Funding/competing Interest(s): This work was supported by the Joint Construction Project of Henan Medical Science and Technology Research Plan under grant number LHGJ20190389. The authors report no conflict of interest.

Trial Registration Number: N/A.
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http://dx.doi.org/10.1093/humrep/deab028DOI Listing
April 2021

Long Noncoding RNA FOXP4-AS1 Predicts Unfavourable Prognosis and Regulates Proliferation and Invasion in Hepatocellular Carcinoma.

Biomed Res Int 2021 1;2021:8850656. Epub 2021 Feb 1.

Department of Ultrasound, Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, China.

Background: Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer that has a high level of morbidity and mortality. Long noncoding RNA (lncRNA) is a novel regulatory factor of tumour proliferation, apoptosis, and metastasis. Our previous studies indicated that lncRNA FOXP4-AS1 is a functional oncogene in HCC; thus, this study is aimed at further evaluating the clinical and biological function of FOXP4-AS1 in HCC. . First, we detected the expression of FOXP4-AS1 in HCC tissues and paracarcinoma normal tissues by qRT-PCR. Second, the prognostic effects of FOXP4-AS1 in patients with HCC were analysed in a training group and a verification group. Subsequently, to investigate the biological effects of FOXP4-AS1 on HCC cells, downexpression tests were further conducted.

Results: The expression of FOXP4-AS1 was higher in HCC tissues than adjacent nontumourous tissues, whereas the low expression of FOXP4-AS1 was correlated with optimistic treatment outcomes, which suggested that FOXP4-AS1 may be an independent prognostic biomarker for HCC. Moreover, the downregulation of FOXP4-AS1 significantly reduced the cell proliferation and clonal abilities and inhibited the invasion, migration, and angiogenesis of hepatoma cells ( < 0.05).

Conclusion: These results revealed the clinical significance and biological function of FOXP4-AS1 in HCC development, which may provide a new direction for finding therapeutic targets and potential prognostic biomarkers of HCC.
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http://dx.doi.org/10.1155/2021/8850656DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7870313PMC
May 2021

Effect of somatometric parameters on the prevalence and severity of varicocele: a systematic review and meta-analysis.

Reprod Biol Endocrinol 2021 Jan 20;19(1):11. Epub 2021 Jan 20.

Henan Human Sperm Bank, The Third Affiliated Hospital of Zhengzhou University, No.7 Front Kangfu Street, Er'qi District, Zhengzhou, 450052, People's Republic of China.

Background: Published studies have shown contradictory results regarding the relationship between somatometric parameters and varicoceles. We performed a systematic review and meta-analysis to investigate the possible effects of age, height, weight, and body mass index (BMI) on the presence and severity of varicoceles.

Methods: Databases including EMBASE, MEDLINE, PubMed, Cochrane Library, China National Knowledge Infrastructure (CNKI), Web of Science, and Google Scholar were systematically searched to identify relevant articles published up to March 2020. Two researchers independently identified eligible articles and extracted data. Cochran's Q statistic and I statistics were used to assess heterogeneity. Meta-analysis was performed using StataSE 12.0 software (StataCorp LP, USA). Random-effects models were used to obtain the weighted mean differences (WMDs) and 95% confidence intervals (CIs). Publication bias was assessed using Begg's funnel plot and Egger's regression test.

Results: The search strategy produced 272 articles, of which 18 articles were eligible according to the inclusion/exclusion criteria. A total of 56,325 patients with varicocele and 1,334,694 patients without varicocele were included in the meta-analysis to evaluate the effect of somatometric parameters on the presence and severity of varicocele. The overall results demonstrated that the presence of varicoceles was significantly associated with height (WMD = 1.41, 95% CI = 1.07 to 1.74, P < 0.001) and inversely correlated with BMI (WMD = - 1.35, 95% CI = -1.67 to - 1.03, P < 0.001) but not with age (WMD = -0.93, 95% CI = -2.19 to 0.33, P = 0.149) or weight (WMD = 0.24, 95% CI = -2.24 to 2.72, P = 0.850). The severity of varicocele was inversely correlated with increased BMI but not with age.

Conclusion: The presence of varicoceles was significantly associated with height and inversely correlated with BMI.
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http://dx.doi.org/10.1186/s12958-021-00695-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816346PMC
January 2021

A recurrent neural network using historical data to predict time series indoor PM2.5 concentrations for residential buildings.

Indoor Air 2021 Jan 15. Epub 2021 Jan 15.

Department of Cardiology, Tianjin Medical University General Hospital, Tianjin, China.

Due to the severe outdoor PM2.5 pollution in China, many people have installed air-cleaning systems in homes. To make the systems run automatically and intelligently, we developed a recurrent neural network (RNN) that uses historical data to predict the future indoor PM2.5 concentration. The RNN architecture includes an autoencoder and a recurrent part. We used data measured in an apartment over the course of an entire year to train and test the RNN. The data include indoor/outdoor PM2.5 concentration, environmental parameters and time of day. By comparing three different input strategies, we found that a strategy employing historical PM2.5 and time of day as inputs performed best. With this strategy, the model can be applied to predict the relatively stable trend of indoor PM2.5 concentration in advance. When the input length is 2 h and the prediction horizon is 30 min, the median prediction error is 8.3 µg/m for the whole test set. For times with indoor PM2.5 concentrations between (20,50] µg/m and (50,100] µg/m , the median prediction error is 8.3 and 9.2 µg/m , respectively. The low prediction error between the ground-truth and predicted values shows that the RNN can predict indoor PM2.5 concentrations with satisfactory performance.
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http://dx.doi.org/10.1111/ina.12794DOI Listing
January 2021

Tumor cell-activated "Sustainable ROS Generator" with homogeneous intratumoral distribution property for improved anti-tumor therapy.

Theranostics 2021 1;11(1):379-396. Epub 2021 Jan 1.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.

Photodynamic therapy (PDT) holds a number of advantages for tumor therapy. However, its therapeutic efficiency is limited by non-sustainable reactive oxygen species (ROS) generation and heterogeneous distribution of photosensitizer (PS) in tumor. Herein, a "ustainable OS enerator" (SRG) is developed for efficient antitumor therapy. SRG was prepared by encapsulating small-sized MnO-Ce6 nanoparticles (MC) into dendritic mesoporous silica nanoparticles (DMSNs) and then enveloped with hyaluronic acid (HA). Due to the high concentration of HAase in tumor tissue, the small-sized MC could be released from DMSNs and homogeneously distributed in whole tumor. Then, the released MC would be uptaken by tumor cells and degraded by high levels of intracellular glutathione (GSH), disrupting intracellular redox homeostasis. More importantly, the released Ce6 could efficiently generate singlet oxygen (O) under laser irradiation until the tissue oxygen was exhausted, and the manganese ion (Mn) generated by degraded MC would then convert the low toxic by-product (HO) of PDT to the most harmful ROS (·OH) for sustainable and recyclable ROS generation. MC could be homogeneously distributed in whole tumor and significantly reduced the level of intracellular GSH. At 2 h after PDT, obvious intracellular ROS production was still observed. Moreover, during oxygen recovery in tumor tissue, ·OH could be continuously produced, and the nanosystem could induce 82% of cell death comparing with 30% of cell death induced by free Ce6. For PDT, SRG achieved a complete inhibition on tumor growth. Based on these findings, we conclude that the designed SRG could induce sustainable ROS generation, homogeneous intratumoral distribution and intracellular redox homeostasis disruption, presenting an efficient strategy for enhanced ROS-mediated anti-tumor therapy.
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http://dx.doi.org/10.7150/thno.50028DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7681092PMC
January 2021

Efficacy and safety of Qishen granules for chronic heart failure: A protocol for systematic review and meta-analysis.

Medicine (Baltimore) 2020 Dec;99(52):e23901

School of Traditional Chinese Medicine, Beijing University of Chinese Medicine.

Background: Qishen granules (QSG) is a famous traditional Chinese Medicine (TCM) formula used to treat chronic heart failure (CHF). The objective of this protocol is to clarify the efficacy and safety of QSG for treating CHF.

Methods: Six databases will be electronically searched up to November 1, 2020 for randomized controlled trials (RCTs) in English and Chinese languages. Two independent reviewers will complete tasks of literature retrieval and data extraction. After that, the Cochrane Collaboration risk of bias tool will be utilized to assess methodological quality. The primary outcomes are left ventricular ejection fraction, left ventricular fractional shortening, and N-terminal B-type natriuretic peptide. The secondary outcomes consist of composite cardiac events, adverse effects, and quality of life. Meta-analysis will be performed using the Revman version 5.3.

Results: This study will provide a high-quality synthesis of current evidence of QSG for CHF from primary and secondary outcomes.

Conclusion: This study will provide evidence for the effectiveness and safety of QSG in the treatment of CHF.

Prospero Registration Number: CRD42020150442.
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http://dx.doi.org/10.1097/MD.0000000000023901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7769332PMC
December 2020

A field investigation of the thermal environment and adaptive thermal behavior in bedrooms in different climate regions in China.

Indoor Air 2020 Dec 17. Epub 2020 Dec 17.

The Bartlett School of Construction and Project Management, University College London (UCL), London, UK.

Sleep thermal environments substantially impact sleep quality. To study the sleep thermal environment and thermal comfort in China, this study carried out on-site monitoring of thermal environmental parameters in peoples' homes, including 166 households in five climate zones, for one year. A questionnaire survey on sleep thermal comfort and adaptive behavior was also conducted. The results showed that the indoor temperature for sleep in northern China was more than 4°C higher than that in southern China in winter, while the indoor temperatures for sleep were similar in summer. Furthermore, 70% of people were satisfied with their sleep thermal environment. Due to the use of air conditioning and window opening in various areas in summer, people were satisfied with their sleep thermal environments. Due to the lack of central heating in the southern region in winter, people feel cold and their sleep thermal environment needs further improvement. The bedding insulation in summer and winter in northern China was 1.83clo and 2.67clo, respectively, and in southern China was 2.21clo and 3.17clo, respectively. Both northern China and southern China used air conditioning only in summer. People in southern China opened their windows all year, while those in northern China opened their windows during the summer and transitional periods.
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http://dx.doi.org/10.1111/ina.12775DOI Listing
December 2020

Metal-Free Trifluoromethylthiolation of Arylazo Sulfones.

J Org Chem 2021 Jan 22;86(1):1292-1299. Epub 2020 Dec 22.

Tianjin Key Laboratory of Structure and Performance for Functional Molecules, College of Chemistry, Tianjin Normal University, Tianjin 300387, People's Republic of China.

A visible-light-driven protocol for the synthesis of aryl trifluoromethyl thioethers under photocatalyst- and metal-free conditions has been pursued. The procedure exploits the peculiar properties of arylazo sulfones (having electron-rich or electron-poor substituents on the (hetero)aromatic ring) as photochemical precursors of aryl radicals and -trifluoromethyl arylsulfonothioates as easy-to-handle trifluoromethylthiolating agents.
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http://dx.doi.org/10.1021/acs.joc.0c02669DOI Listing
January 2021

NOX1/NADPH oxidase affects the development of autism-like behaviors in a maternal immune activation model.

Biochem Biophys Res Commun 2021 01 10;534:59-66. Epub 2020 Dec 10.

Department of Pharmacology, Kyoto Prefectural University of Medicine, Kyoto, 602-8566, Japan. Electronic address:

Autism spectrum disorder (ASD) is a neurodevelopmental disorder caused by genetic and environmental factors. Among the environmental factors, maternal infection is known as one of the principal risk factors for ASD. On the other hand, postmortem studies suggested the relationship of oxidative stress with ASD etiology. However, the role of oxidative stress in the development of ASD remains unclear. Here, we report the involvement of NOX1/NADPH oxidase, an enzyme generating reactive oxygen species (ROS), in behavioral and anatomical abnormalities in a maternal immune activation (MIA) model. In the MIA model of gestational polyinosinic-polycytidylic acid (poly(I:C)) exposure, increased serum levels of IL-6 were observed in both wild-type (WT) and Nox1-deficient mice (Nox1KO). Following the comparable induction of MIA in the two genotypes, impairment of social preference and defects in motor coordination were observed in WT offspring but not in offspring deficient in Nox1. MIA up-regulated NOX1 mRNA in the cerebral cortex and cerebellum of the fetus but not in the adult offspring. Although the development of cortical neurons was unaffected by MIA in either genotype, the dropout of Purkinje cells in lobule VII of MIA-affected offspring was significantly ameliorated in Nox1KO. Taken together, these results suggested that NOX1/NADPH oxidase plays an essential role in some behavioral phenotypes observed in ASD, possibly by promoting the loss of Purkinje cells in the cerebellum.
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http://dx.doi.org/10.1016/j.bbrc.2020.11.070DOI Listing
January 2021