Publications by authors named "Junhong Ma"

27 Publications

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Long non-coding RNA PART1 predicts a poor prognosis and promotes the malignant progression of pancreatic cancer by sponging miR-122.

World J Surg Oncol 2021 Apr 17;19(1):122. Epub 2021 Apr 17.

Department of Gastrointestinal Surgery, Tianjin Nankai Hospital, Tianjin, 300100, China.

Background And Objectives: Long non-coding RNA (lncRNA) prostate androgen-regulated transcript 1 (PART1) was previously shown to exert an oncogenic role in several human cancers. However, whether PART1 is associated with the malignant progression of pancreatic cancer remains unclear. In the current study, we aimed to identify the role and potential mechanism of PART1 in pancreatic cancer.

Methods: qRT-PCR was applied to detect PART1 expression in 45 cases of pancreatic cancer patients. The chi-square test was performed to assess the association between PART1 expression and clinicopathologic features, and Kaplan-Meier method was applied to evaluate overall survival. In vitro CCK-8, transwell invasion, and flow cytometry assays were applied to detect the effects of PART1 on cell proliferation, invasion, and apoptosis, respectively. Luciferase reporter and RNA immunoprecipitation assays were used to identify the regulatory mechanism between PART1 and miR-122.

Results: PART1 expression was upregulated in pancreatic cancer tissues and cell lines. High PART1 expression was closely correlated with tumor size, T classification, clinical stage, and vascular invasion, and predicted a poor overall survival. PART1 knockdown significantly suppressed cell proliferation and invasion abilities of pancreatic cancer but promoted cell apoptosis. PART1 was found to serve as a molecular sponge of miR-122, and miR-122 inhibition partially reversed the inhibitory phenotypes of PART1 knockdown on pancreatic cancer cells.

Conclusions: PART1 promotes the malignant progression of pancreatic cancer by sponging miR-122. The PART1/miR-122 axis might be a promising target for anticancer therapy in patients with pancreatic cancer.
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http://dx.doi.org/10.1186/s12957-021-02232-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053290PMC
April 2021

Reliability and Validity of the Simplified Chinese Version of the Aberrant Behavior Checklist in Chinese Autism Population.

Front Psychiatry 2020 14;11:545445. Epub 2020 Oct 14.

Institute of Mental Health, Peking University Sixth Hospital, Beijing, China.

The Aberrant Behavior Checklist (ABC) is a widely used scale in autism clinical intervention research for the assessment of core symptoms and comorbid emotional and behavioral problems among people with autism. The aim of this study was to examine the psychometric properties of the Simplified Chinese version of the Aberrant Behavior Checklist (SC-ABC) using a sample of people with autism in a Chinese population. In total, we enrolled 799 patients aged 1.5-33 years old. We collected data using the SC-ABC ( = 799), Autism Behavior Checklist ( = 743), Attention Deficit Hyperactivity Disorder Rating Scale-IV (ADHD-RS-IV) ( = 433) and Achenbach Child Behavior Checklist (CBCL) ( = 319). Eighty-four patients were separately assessed with the SC-ABC by two caregivers simultaneously. Forty-four patients were assessed with the SC-ABC again by same caregiver 2 weeks after the first assessment. SC-ABC data from the whole sample were used for confirmatory factor analysis. We evaluated criterion validity using Spearman's correlation coefficient between scores of the SC-ABC and scores of the Autism Behavior Checklist, ADHD-RS-IV and CBCL separately in the whole sample and different age groups. We calculated the intragroup correlation coefficients and Spearman's correlation coefficient for interrater reliability in 84 samples and test-retest reliability in 44 samples. We conducted Cronbach's α for internal consistency. For the SC-ABC, the intragroup correlation coefficients of five subscales and the total score in interrater and test-retest reliability ranged from 0.87 to 0.92 and from 0.93 to 0.97 (all < 0.01). The Spearman's correlation coefficient of five subscales and the total score in interrater and test-retest reliability ranged from 0.78 to 0.85 and 0.86 to 0.94, respectively (all < 0.01). Cronbach's α of five subscales and the total score ranged from 0.75 to 0.96 (all < 0.01). The Spearman's correlation coefficient for criterion validity for the whole sample and different age groups ranged from 0.39 to 0.76 (all < 0.01). The model fit for the original five factor model was acceptable, with fit indices of SMR = 0.062 and RMSEA = 0.052. The SC-ABC has satisfactory psychometric properties and can be used in the assessment of core symptoms and comorbid emotional and behavioral problems in patients with autism.
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http://dx.doi.org/10.3389/fpsyt.2020.545445DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7591387PMC
October 2020

Multiomics Integrative Analysis for Discovering the Potential Mechanism of Dioscin against Hyperuricemia Mice.

J Proteome Res 2021 01 27;20(1):645-660. Epub 2020 Oct 27.

State Key Laboratory of Component-Based Chinese Medicine, Tianjin Key Laboratory of TCM Chemistry and Analysis, 10 Poyanghu Road, Jinghai District, Tianjin 301617, China.

Hyperuricemia is a well-known key risk factor for gout and can cause a variety of metabolic diseases. Several studies have shown that dioscin could improve metabolic symptoms and reduce the uric acid level in blood. However, there is no comprehensive metabolomic study on the anti-hyperuricemia effects of dioscin. A total of 29 adult male Kunming mice were divided into three groups: Normal (blank), PO (potassium oxonate-administrated, 200 mg/kg/day), and Dioscin (potassium oxonate + dioscin, potassium oxonate 200 mg/kg/day, dioscin 50 mg/kg/day). All mice were treated for 42 days via oral gavage. This paper implemented an untargeted metabolomics study based on H NMR and LC-MS to discover the comprehensive mechanism of dioscin. Furthermore, a targeted lipidomics was fulfilled to further analyze the lipid metabolism disorder. Finally, the metabolic pathway mediated by dioscin was verified at the gene level by means of transcriptomics. The results show 53 different metabolites were closely related to the improvement of dioscin in PO-induced hyperuricemia, and 19 of them were lipids. These metabolites are mainly involved in the tricarboxylic acid cycle, lipid metabolism, amino acid metabolism, and pyrimidine metabolism. According to the transcriptomics study, the levels of 89 genes were significantly changed in the PO group compared to the normal control. Among them, six gene levels were restored by the treatment of dioscin. The six changed genes (tx1b, Tsku, Tmem163, Psmc3ip, Tcap, Tbx15) are mainly involved in the cell cycle and energy metabolism. These metabolites and genes might provide useful information for further study of the therapeutic mechanism of dioscin.
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http://dx.doi.org/10.1021/acs.jproteome.0c00584DOI Listing
January 2021

Response to antiepileptic drugs after unsuccessful epilepsy surgery: A multivariate analysis of 103 patients.

Seizure 2020 Oct 18;81:222-227. Epub 2020 Aug 18.

Beijing Institute of Brain Disorders, Laboratory of Brain Disorders, Ministry of Science and Technology, Collaborative Innovation Center for Brain Disorders, Capital Medical University, Beijing, China; Department of Neurology, the First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, 1Youyi Road, Chongqing, 400016, China. Electronic address:

Object: Epilepsy patients may still have seizures after surgery, and there have been few studies on the response to antiepileptic drugs (AEDs) after surgery failure. The purpose of this study was to analyze the response to AEDs after unsuccessful epilepsy surgery.

Methods: Patients who underwent unsuccessful epilepsy surgery between January 1999 and January 2019 were evaluated. Patient demographics, etiology, factors related to surgery and AED use patterns were assessed.

Results: After excluding the 5 patients who were lost to follow-up and the 2 patients who died, the records of 103 consecutive patients were analyzed. Ninety patients (87.4 %) had seizure recurrence within one year after surgery, 2 (1.9 %) patients had recurrence from one year to two years after surgery, and 11 (10.7 %) patients had recurrence two or more years after surgery (2-10 years). After surgery failure, the patients tried at least 2 kinds of AEDs with different mechanisms for more than 2 years. The average total number of AEDs used was 5.97, the average number of AEDs used before surgery was 3.21, and the average number of AEDs used after surgery was 4.02. After retreatment with AEDs, 10 patients (9.7 %) were seizure-free, 18 patients' (17.5 %) seizures were alleviated, and 75 patients (72.8 %) had seizures as they did prior to the adjustments. The number of AEDs used before and after surgery and the total number of AEDs were not significantly different among the seizure free group, alleviated seizure group and no change group. There were no significant differences in seizure onset age, surgery age, etiology, time between seizure onset and surgery, magnetic resonance imaging, seizure type, localization and lateralization of the surgery site among the three groups.

Conclusions: The results showed that a small percentage of patients (27.2 %) who undergo unsuccessful epilepsy surgery benefit from AED adjustments; however, the vast majority of patients (72.8 %) do not benefit from AED adjustments.
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http://dx.doi.org/10.1016/j.seizure.2020.08.014DOI Listing
October 2020

Serum Exosomal Proteins F9 and TSP-1 as Potential Diagnostic Biomarkers for Newly Diagnosed Epilepsy.

Front Neurosci 2020 4;14:737. Epub 2020 Aug 4.

Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Chongqing, China.

Epilepsy is one of the most common chronic neurological diseases in the world, with a high incidence, a high risk of sudden unexplained death, and diagnostic challenges. Exosomes are nanosized extracellular vesicles that are released into physical environments and carry a variety of biological information. Moreover, exosomes can also be synthesized and released from brain cells, passing through the blood-brain barrier, and can be detected in peripheral blood or cerebrospinal fluid. Our study using the tandem mass tag (TMT) approach showed that a total of 76 proteins were differentially expressed in serum exosomes between epilepsy patients and healthy controls, with 6 proteins increasing and 70 proteins decreasing. Analysis of large clinical samples and two mouse models of chronic epilepsy indicated that two significantly differentially expressed serum exosomal proteins, coagulation factor IX (F9) and thrombospondin-1 (TSP-1), represent promising biomarkers for the diagnosis of epilepsy, with area under the curve (AUC) values of up to 0.7776 (95% CI, 0.7306-0.8246) and 0.8534 (95% CI, 0.8152-0.8916), respectively. This is the first study of exosomal proteins in epilepsy, and it suggests that exosomes are promising new tools for the diagnosis of epilepsy.
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http://dx.doi.org/10.3389/fnins.2020.00737DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7417627PMC
August 2020

A Rare Missense Mutation Enhances Synaptic Function and Increases Seizure Activity.

Front Genet 2020 27;11:61. Epub 2020 Feb 27.

Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Laboratory of Neurology, Chongqing, China.

Although genetic factors are considered a main etiology of epilepsy, the causes of genetic epilepsy in the majority of epilepsy patients remain unknown. Kinesin family member 1A (KIF1A), a neuron-specific motor protein that moves along with microtubules, is responsible for the transport of membranous organelles and synaptic vesicles. Variants of have recently been associated with hereditary spastic paraplegia (HSP), hereditary sensory and autonomic neuropathy type 2 (HSANII), and intellectual disability. However, mutations in have not been detected in patients with epilepsy. In our study, we conducted customized sequencing of epilepsy-related genes of a family with six patients with generalized epilepsy over three generations and identified a rare heterozygous mutation (c.1190C > A, p. Ala397Asp) in . Whole-cell recordings from primary cultured neurons revealed that the mutant increases the excitatory synaptic transmission but not the intrinsic excitability of neurons, and phenotype testing in zebrafish showed that this rare mutation results in epileptic seizure-like activity. These results provide new evidence demonstrating that dysfunction is involved in epileptogenesis.
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http://dx.doi.org/10.3389/fgene.2020.00061DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7056823PMC
February 2020

Photodynamic therapy for squamous cell carcinoma of the index finger: A case report.

Authors:
Junhong Ma Xin Wang

Photodiagnosis Photodyn Ther 2020 Mar 13;29:101661. Epub 2020 Jan 13.

Department of Dermatology, The First Affiliated Hospital of Tsinghua University, Beijing, China.

Skin squamous cell carcinoma (SCC) is the second most common skin cancer, and surgical resection is the first choice of treatment. However, some special sites such as lips, eyelids, distal fingers and genital areas are not suitable for operation. We report a case of SCC on his left index for three years. Concerning the side effects such as scarring and functional defects, the patient refused operation. We conducted four sessions of topical 5-aminolevulinic acid photodynamic therapy (ALA-PDT), at 7-10 day intervals, the skin lesion healed. After twelve months of follow-up, the lesion was clinically cleared without evidence of recurrence. Thus, we believe that PDT can be an alternative treatment for SCC occurring on fingers without scar contracture and loss of digital function.
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http://dx.doi.org/10.1016/j.pdpdt.2020.101661DOI Listing
March 2020

Inhibition of Nwd1 activity attenuates neuronal hyperexcitability and GluN2B phosphorylation in the hippocampus.

EBioMedicine 2019 Sep 29;47:470-483. Epub 2019 Aug 29.

Department of Neurology, Zhujiang Hospital of Southern Medical University, Gongye Road 253, Guangzhou, Guangdong Province 510282, PR China. Electronic address:

Background: NACHT and WD repeat domain-containing protein 1 (Nwd1) is a member of the innate immune protein subfamily. Nwd1 contributes to the androgen receptor signaling pathway and is involved in axonal growth. However, the mechanisms that underlie pathophysiological dysfunction in seizures remain unclear.

Methods: Biochemical methods were used to assess Nwd1 expression and localization in a mouse model of kainic acid (KA)-induced acute seizures and temporal lobe epilepsy (TLE) patients. Electrophysiological recordings were used to measure the role of Nwd1 in regulating synaptic transmission and neuronal hyperexcitability in a model of magnesium-free-induced seizure in vitro. Behavioral experiments were performed, and seizure-induced pathological changes were evaluated in a KA-induced seizure model in vivo. GluN2B expression was measured and its correlation with Tyr1472-GluN2B phosphorylation was analyzed in primary hippocampal neurons.

Findings: We demonstrated high protein levels of Nwd1 in brain tissues obtained from mice with acute seizures and TLE patients. Silencing Nwd1 in mice using an adeno-associated virus (AAV) profoundly suppressed neuronal hyperexcitability and the occurrence of acute seizures, which may have been caused by reducing GluN2B-containing NMDA receptor-dependent glutamatergic synaptic transmission. Moreover, the decreased activation of Nwd1 reduced GluN2B expression and the phosphorylation of the GluN2B subunit at Tyr1472.

Interpretation: Here, we report a previously unrecognized but important role of Nwd1 in seizure models in vitro and in vivo, i.e., modulating the phosphorylation of the GluN2B subunit at Tyr1472 and regulating neuronal hyperexcitability. Meanwhile, our findings may provide a therapeutic strategy for the treatment of epilepsy or other hyperexcitability-related neurological disorders. FUND: The funders have not participated in the study design, data collection, data analysis, interpretation, or writing of the report.
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http://dx.doi.org/10.1016/j.ebiom.2019.08.050DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6796588PMC
September 2019

miRNA and mRNA Integration Network Construction Reveals Novel Key Regulators in Left-Sided and Right-Sided Colon Adenocarcinoma.

Biomed Res Int 2019 3;2019:7149296. Epub 2019 Apr 3.

Department of Gastrointestinal Surgery, Nankai Clinical College of Tianjin Medical University, China.

Background: The distinction between right-sided and left-sided colon adenocarcinoma has recently received considerable. This study aims to identify key MicroRNA (miRNA) and mRNAs in right-sided colon adenocarcinoma (RSCOAD) and left-sided colon adenocarcinoma (LSCOAD) by TCGA integration analysis.

Methods: The miRNA and mRNA expression profiles of a large group of patients with RSCOAD and LSCOAD were obtained from TCGA. The differentially expressed miRNAs (DEmiRNAs) and mRNAs (DEmRNAs) were identified by TCGA integration analysis. The optimal diagnostic miRNA biomarkers for RSCOAD and LSCOAD were identified by Boruta algorithm. We established classification models to distinguish RSCOAD and LSCOAD. Protein-protein interaction (PPI) network analysis, DEmiRNA-DEmRNA interaction analysis, and functional annotation were performed. The expression of selected DEmiRNAs and DEmRNAs was validated by qRT-PCR.

Results: A total of 2534 DEmRNAs (940 downregulated and 1594 upregulated mRNAs) and 54 DEmiRNAs (22 downregulated and 32 upregulated miRNAs) between RSCOAD and LSCOAD were identified. The feature selection procedure was to obtain 22 optimal diagnostic miRNAs biomarkers in RSCOAD compared to LSCOAD. The AUC of the random forests model was 0.869 and the specificity and sensitivity of this model were 79% and 84.6%, respectively. Three DEmiRNAs (hsa-miR-224-5p, hsa-miR-155-5p, and hsa-miR-31-5p) and five DEmRNAs (CXCR4, SMAD4, KRAS, FITM2, and PLAGL2) were identified key DEmiRNAs and DEmRNAs in RSCOAD compared to LSCOAD. The qRT-PCR results of CXCR4, FITM2, TFAP2A, ULBP2, hsa-miR-224-5p, and hsa-miR-155-5p were consistent with our integrated analysis.

Conclusion: A total of three DEmiRNAs (hsa-miR-224-5p, hsa-miR-155-5p, and hsa-miR-31-5p) and five DEmRNAs (CXCR4, SMAD4, KRAS, FITM2, and PLAGL2) may be involved in the pathogenesis of RSCOAD and LSCOAD which may make a contribution for understanding mechanisms and developing therapeutic strategies for RSCOAD and LSCOAD.
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http://dx.doi.org/10.1155/2019/7149296DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6470432PMC
September 2019

Lycorine Displays Potent Antitumor Efficacy in Colon Carcinoma by Targeting STAT3.

Front Pharmacol 2018 8;9:881. Epub 2018 Aug 8.

Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Signal transducer and activator of transcription 3 (STAT3) is an attractive therapeutic target for cancer treatment. In this study, we identify lycorine is an effective inhibitor of STAT3, leading to repression of multiple oncogenic processes in colon carcinoma. Lycorine selectively inactivates phospho-STAT3 (Tyr-705), and subsequent molecular docking uncovers that lycorine directly binds to the SH2 domain of STAT3. Consequently, we find that lycorine exhibits anti-proliferative activity and induces cell apoptosis on human colorectal cancer (CRC) . Lycorine induces the activation of the caspase-dependent mitochondrial apoptotic pathway, as indicated by activation of caspase and increase of the ratio of Bax/Bcl-2 and mitochondrial depolarization. Overexpressing STAT3 greatly blocks these effects by lycorine in CRC cells. Finally, lycorine exhibits a potential therapeutic effect in xenograft colorectal tumors by targeting STAT3 without observed toxicity. Taken together, the present study indicates that lycorine acts as a promising inhibitor of STAT3, which blocks tumorigenesis in colon carcinoma.
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http://dx.doi.org/10.3389/fphar.2018.00881DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6092588PMC
August 2018

Biomass based iron and nitrogen co-doped 3D porous carbon as an efficient oxygen reduction catalyst.

J Colloid Interface Sci 2018 Aug 28;523:144-150. Epub 2018 Mar 28.

College of Chemistry and Chemical Engineering, Xinjiang University, Urumqi 830046, People's Republic of China.

An iron and nitrogen co-doped 3D porous carbon catalyst with high performance for oxygen reduction reaction (ORR) is produced by pyrolysis. The precursor is a mixture of red date, ferrous (Ⅱ) acetate, and graphitic carbon nitride (g-CN). g-CN is the nitrogen source and also the sacrificial template, which plays a key role in the formation of a porous nitrogen rich carbon structure with high surface area. It is found that active sites and catalytic performance of synthetic materials are significantly influenced by the Fe content. In comparison with commercial Pt/C catalyst, the synthetic Fe-N-C with optimized composition exhibits comparable ORR activity and superior methanol tolerance. This work provides a feasible approach to develop cost-effective and highly efficient non-precious metal electrochemical catalysts for oxygen reduction.
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http://dx.doi.org/10.1016/j.jcis.2018.03.092DOI Listing
August 2018

Peripheral CD4+ naïve/memory ratio is an independent predictor of survival in non-small cell lung cancer.

Oncotarget 2017 Oct 18;8(48):83650-83659. Epub 2017 Jul 18.

Department of Thoracic Surgery, Linyi People's Hospital, Linyi 276000, China.

Background: To investigate the clinical significance of naïve T cells, memory T cells, CD45RA+CD45RO+ T cells, and naïve/memory ratio in non-small cell lung cancer (NSCLC) patients.

Methods: Pretreatment peripheral blood samples from 76 NSCLC patients and 28 age- and sex-matched healthy volunteers were collected and tested for immune cells by flow cytometry. We compared the expression of these immune cells between patients and healthy controls and evaluated their predictive roles for survival in NSCLC by cox proportional hazards model.

Results: Decreased naïve CD4+ T cells, naïve CD8+ T cells, CD4+ naïve/memory ratios and CD4+CD45RA+CD45RO+ T cells, and increased memory CD4+ T cells, were observed in 76 NSCLC patients compared to healthy volunteers. Univariate analysis revealed that elevated CD4+ naïve/memory ratio correlated with prolonged progression-free survival (P=0.013). Multivariate analysis confirmed its predictive role with a hazard ratio of 0.35 (95% confidence interval, 0.19-0.75, P=0.012).

Conclusions: Peripheral CD4+ naïve/memory ratio can be used as a predictive biomarker in NSCLC patients and used to optimize personalized treatment strategies.
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http://dx.doi.org/10.18632/oncotarget.19330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5663543PMC
October 2017

Hierarchical Hybrids Integrated by Dual Polypyrrole-Based Porous Carbons for Enhanced Capacitive Performance.

Chemistry 2017 Sep 5;23(54):13474-13481. Epub 2017 Sep 5.

School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an, 710049, P.R. China.

Architectural design of nitrogenous polymer-based carbons represents a facile and efficient strategy to improve performance because of their morphological diversity, tailorability, and N-containing structure. In this research, 0D and 1D forms of polypyrrole-derived porous carbons (A-PCS and A-PCT, respectively) are first integrated into nitrogen-doped hierarchically porous A-PCS/PCT hybrids by applying an easy multistep method. This integration, along with chemical activation, prevents serious agglomeration of carbon particles or tubes and creates a connected porous network structure, leading to improved textural properties (high surface area of 1684 m  g , high pore volume of 1.57 cm  g , and hierarchical porosity). Thus, A-PCS/PCT hybrids in a three-electrode setup reach high specific capacitances of 224 and 206 F g at 1 and 20 A g , respectively, with high rate capability (92 % capacitance retention). A symmetrical supercapacitor with A-PCS/PCT electrodes presents the highest power and energy densities of 12.6 kW kg and 8.58 Wh kg , respectively, and exceptional cycling life and stability with 92.4 % retention for up to 20 000 cycles. This study on conductive polymer-based hybrid materials may guide the design of architectures with new structures for applications in energy storage and conversion technologies.
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http://dx.doi.org/10.1002/chem.201702544DOI Listing
September 2017

Identification of the long non‑coding RNA LET as a novel tumor suppressor in gastric cancer.

Mol Med Rep 2017 Apr 28;15(4):2229-2234. Epub 2017 Feb 28.

School of Management, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, P.R. China.

Long non-coding RNAs (lncRNAs) have emerged recently as important factors in regulating fundamental biological processes. Alterations in the expression and function of lncRNAs have been observed to promote tumor formation, progression and metastasis. Although downregulation of the expression levels of LET lncRNA in several tumors has been reported, its role in gastric cancer remains unknown. The aim of the present study was to investigate the expression and function of LET in gastric cancer development. The expression levels of LET in 37 pairs of gastric cancer and adjacent non‑tumor tissues were detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). In addition, LET expression in gastric cancer cell lines was analyzed by RT‑qPCR assay analysis. Furthermore, the impact of LET on cell proliferation, migration and apoptosis were detected using the cell counting kit‑8, wound scratch and ELISA assays, respectively. The results demonstrated that the expression level of LET was downregulated in gastric cancer tissues and cell lines (SGC‑7901 and MGC‑803) compared with normal tissues and a normal human gastric epithelial cell line (GES‑1). Restoration of LET expression using a synthesized recombinant overexpression vector transfected into SGC‑7901 and MGC‑803 cells, significantly inhibited cell proliferation and migration, and promoted cell apoptosis in vitro. The present study is the first to demonstrate that LET may function as a tumor suppressor in gastric cancer. The results indicate that LET may be a promising biomarker and/or a therapeutic target for gastric cancer.
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http://dx.doi.org/10.3892/mmr.2017.6263DOI Listing
April 2017

Sandwich-Like [email protected]@C Coaxial Nanocables with Enhanced Lithium-Storage Capability.

ACS Appl Mater Interfaces 2017 Jan 5;9(2):1453-1458. Epub 2017 Jan 5.

School of Chemistry and Chemical Engineering and ‡Key Laboratory of Energy Material Chemistry, Ministry of Education, Institute of Applied Chemistry, Xinjiang University , Urumqi 830046, Xinjiang China.

Through the combined method of a low-temperature reflux and calcination, porous sandwich-like [email protected]@C coaxial nanocables were cleverly constructed, which exhibited a favorable specific capacity of 724.8 mA h g at 1000 mA g, a satisfying rate performance and admirable Coulombic efficiency (ca. 100%) for anodes of lithium-ion batteries. Due to the enlarged contact surface area, shortened Li diffusion distance, hierarchical porosity, reasonable structural design and good structural stability, the electrochemical performance of the [email protected]@C nanocomposites was greatly enhanced in comparison with the traditional iron oxide anodes. So, it is a good candidate for anode materials with high performance.
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http://dx.doi.org/10.1021/acsami.6b12482DOI Listing
January 2017

Hybrid porous bamboo-like CNTs embedding ultrasmall LiCrTiO nanoparticles as high rate and long life anode materials for lithium ion batteries.

Chem Commun (Camb) 2017 Jan;53(6):1033-1036

Key Laboratory of Energy Materials Chemistry, Ministry of Education, Institute of Applied Chemistry, Xinjiang University, Urumqi 830046, Xinjiang, China.

Compared with LiTiO, LiCrTiO with the same spinel structure exhibits superior conductivity and Li diffusion properties. However, there has been no extensive study on LiCrTiO as an anode material in lithium ion batteries, due to its pulverization, loss of electrical contact, and particle aggregation. A unique architecture based on hybrid porous CNTs embedding ultrasmall LiCrTiO nanoparticles (6 ± 2 nm) was designed by using a facile sol-gel process combined with subsequent heat treatment. As an anode for lithium ion batteries, due to the absence of the aforementioned problems, the electrode exhibited high reversible capacity, excellent rate capability and superior long-term cycling stability at high current densities. Such nanocomposites should be competitive candidates to replace LiTiO-based anode materials.
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http://dx.doi.org/10.1039/c6cc08103gDOI Listing
January 2017

Identification a novel tumor-suppressive hsa-miR-599 regulates cells proliferation, migration and invasion by targeting oncogenic MYC in hepatocellular carcinoma.

Am J Transl Res 2016 15;8(6):2575-84. Epub 2016 Jun 15.

Tianjin University of Traditional Chinese Medicine Tianjin 300193, P. R. China.

Increasing evidences have demonstrated that microRNAs (miRNAs) act an essential role in regulating tumor progression and metastasis. Previous miRNAs microarray data showed that hsa-miR-599 is lower expressed in hepatocellular carcinoma (HCC); however, the function and molecular mechanism of hsa-miR-599 on HCC has not been well illustrated. Here, we first analyzed the expression level of hsa-miR-599 in HCC tissues and cell lines by real-time reverse-transcription PCR (qRT-PCR). Interestingly, we found that hsa-miR-599 was significantly down-regulated in the examined HCC tissues and cell lines. Then cells proliferation, migration and invasion were assessed by MTT, wound-healing and trans-well assay respectively. The results showed that over-expression of hsa-miR-599 resulted in inhibited HCC cells proliferation, migration and invasion in vitro. In addition, dual-luciferase reporter assay, qRT-PCR and Western blot analyzes were used to confirm MYC (v-myc avian myelocytomatosis viral oncogene homolog) as a target gene of hsa-miR-599. MYC expression was up-regulated in HCC tissues and cell lines, and restoration of hsa-miR-599 could remarkably decreased the mRNA and protein levels of MYC. Moreover, over-expression of MYC partly reversed hsa-miR-599-mediated inhibition of HCC cells proliferation, migration and invasion in vitro. Taken together, our data demonstrate that hsa-miR-599 acts as a tumor suppressor and inhibits HCC cells proliferation, migration and invasion by partly targeting oncogenic MYC, which hints that hsa-miR-599 can be a diagnostic and therapeutic biomarker in HCC.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4931152PMC
July 2016

A composite catalyst of reduced black TiO2-x/CNT: a highly efficient counter electrode for ZnO-based dye-sensitized solar cells.

Chem Commun (Camb) 2015 Dec 16;51(98):17459-62. Epub 2015 Oct 16.

Key Laboratory of Oil and Gas Fine Chemicals, Ministry of Education and Xinjiang Uyghur Autonomous Region, Xinjiang University, Urumqi 830046, China.

A composite catalyst (reduced black TiO2-x/carbon nanotube) was synthesized through a simple sol-gel method and applied as a counter electrode (CE) in ZnO-based dye-sensitized solar cells (DSSCs). This material demonstrated notable electrocatalytic activity for I3(-) reduction, and the resultant DSSCs achieved a PCE of 5.71%.
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http://dx.doi.org/10.1039/c5cc07284kDOI Listing
December 2015

p53 controls colorectal cancer cell invasion by inhibiting the NF-κB-mediated activation of Fascin.

Oncotarget 2015 Sep;6(26):22869-79

Biomedical Research Center and Key Laboratory of Biotherapy of Zhejiang Province, Zhejiang University, Hangzhou, Zhejiang, China.

p53 mutation is known to contribute to cancer progression. Fascin is an actin-bundling protein and has been recently identified to promote cancer cell migration and invasion through its role in formation of cellular protrusions such as filopodia and invadopodia. However, the relationship between p53 and Fascin is not understood. Here, we have found a new link between them. In colorectal adenocarcinomas, p53 mutation correlated with high NF-κB, Fascin and low E-cadherin expression. Moreover, this expression profile was shown to contribute to poor overall survival in patients with colorectal cancer. Wild-type p53 could inhibit NF-κB activity that repressed the expression of Fascin and cancer cell invasiveness. In contrast, in p53-deficient primary cultured cells, NF-κB activity was enhanced and then activation of NF-κB increased the expression of Fascin. In further analysis, we showed that NF-κB was a key determinant for p53 deletion-stimulated Fascin expression. Inhibition of NF-κB/p65 expression by pharmacological compound or p65 siRNA suppressed Fascin activity in p53-deficient cells. Moreover, restoration of p53 expression decreased the activation of Fascin through suppression of the NF-κB pathway. Taken together, these data suggest that a negative-feedback loop exists, whereby p53 can suppress colorectal cancer cell invasion by inhibiting the NF-κB-mediated activation of Fascin.
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http://dx.doi.org/10.18632/oncotarget.5137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673205PMC
September 2015

The anticancer immune response of anti-PD-1/PD-L1 and the genetic determinants of response to anti-PD-1/PD-L1 antibodies in cancer patients.

Oncotarget 2015 Aug;6(23):19393-404

Department of Immunology, University of Toronto, Toronto, ON, Canada.

The programmed death-1 (PD-1), a coinhibitory receptor expressed on activated T cells and B cells, is demonstrated to induce an immune-mediated response and play a critical role in tumor initiation and development. The cancer patients harboring PD-1 or PD ligand 1 (PD-L1) protein expression have often a poor prognosis and clinical outcome. Currently, targeting PD-1 pathway as a potential new anticancer strategy is attracting more and more attention in cancer treatment. Several monoclonal antibodies against PD-1 or PD-L1 have been reported to enhance anticancer immune responses and induce tumor cell death. Nonetheless, the precise molecular mechanisms by which PD-1 affects various cancers remain elusive. Moreover, this therapy is not effective for all the cancer patients and only a fraction of patients respond to the antibodies targeting PD-1 or PD-L1, indicating these antibodies may only works in a subset of certain cancers. Thus, understanding the novel function of PD-1 and genetic determinants of response to anti-PD-1 therapy will allow us to develop a more effective and individualized immunotherapeutic strategy for cancer.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4637293PMC
http://dx.doi.org/10.18632/oncotarget.5107DOI Listing
August 2015

Enhanced electrocatalytic activity of Pt nanoparticles supported on functionalized graphene for methanol oxidation and oxygen reduction.

J Colloid Interface Sci 2015 Nov 23;457:102-7. Epub 2015 Jun 23.

Key Laboratory of Advanced Functional Materials, Autonomous Region, Institute of Applied Chemistry, Xinjiang University, Urumqi 830046, People's Republic of China.

Reduced graphene oxide (RGO) is modified with 1,1'-dimethyl-4,4'-bipyridinium dichloride (methyl viologen, i.e. MV) by a π-π interaction, and further used as support for immobilization of Pt nanoparticles to prepare a Pt/MV-RGO catalyst for direct methanol fuel cells. Transmission electron microscopy, X-ray powder diffraction, Raman spectroscopy and X-ray photoelectron spectroscopy are used to characterize the morphology and microstructure of the prepared catalyst. Electrocatalytic performance of the as-prepared Pt/MV-RGO hybrid is investigated by cyclic voltammetry, linear scan voltammetry, COads stripping voltammetry and electrochemical impedance spectroscopy. The Pt/MV-RGO hybrid exhibits much higher catalytic activities than Pt/RGO toward both methanol oxidation and oxygen reduction, suggesting that Pt/MV-RGO hybrid could be a promising electrocatalyst for high-performance direct methanol fuel cells applications.
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http://dx.doi.org/10.1016/j.jcis.2015.06.031DOI Listing
November 2015

An improved MALDI-TOF mass spectrometry procedure and a novel DNA marker for identifying over-expressed Bx7 glutenin protein subunit in wheat.

Hereditas 2014 Dec;151(6):196-200

Australia-China Centre for Wheat Improvement, School of Veterinary and Life Sciences, Murdoch University, Perth, WA, Australia; Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing, China.

Wheat bread-making quality is mainly determined by glutenin proteins in the grain, which exist in a wide range of variable alleles with differential influence on processing attributes. A recently identified allele, Bx7 over-expression (Bx7(oe) ), has been showing highly significant positive effects on wheat dough strength over the normally expressed Bx7 allele. SDS-PAGE and normal RP-HPLC procedures failed to separate the two alleles. In the current study, an extensively optimised MALDI-TOF based procedure and a refined DNA based marker for efficiently differentiating Bx7(oe) from normal Bx7 allele were established. Results indicated that the MALDI-TOF procedure is cost effective, high throughput, and proven reliable, while the refined PCR marker only amplifies Bx7(oe) allele, a clear advantage over the previously developed codominant marker.
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http://dx.doi.org/10.1111/hrd2.00069DOI Listing
December 2014

Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling.

J Exp Bot 2014 Aug 12;65(15):4305-15. Epub 2014 May 12.

Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road 132, 650201, Kunming, China

Alternaria alternata (tobacco pathotype) is a necrotrophic fungus causing severe losses in Nicotiana species by infection of mature leaves. Similar to what has been observed in cultivated tobacco, N. tabacum, young leaves of wild tobacco, N. attenuata, were more resistant to A. alternata than mature leaves, and this was correlated with stronger blue fluorescence induced after infection. However, the nature of the fluorescence-emitting compound, its role in defence, and its regulation were not clear. Silencing feruloyl-CoA 6'-hydroxylase 1 (F6'H1), the gene encoding the key enzyme for scopoletin biosynthesis, by virus-induced gene silencing (VIGS) revealed that the blue fluorescence was mainly emitted by scopoletin and its β-glycoside form, scopolin. Further analysis showed that scopoletin exhibited strong antifungal activity against A. alternata in vitro and in vivo. Importantly, jasmonic acid (JA) levels were highly elicited in young leaves but much less in mature leaves after infection; and fungus-elicited scopoletin was absent in JA-deficient plants, but was largely restored with methyl jasmonate treatments. Consistent with this, plants strongly impaired in JA biosynthesis and perception were highly susceptible to A. alternata in the same way scopoletin/scopolin-depleted VIGS F6'H1 plants. Furthermore, silencing MYC2, a master regulator of most JA responses, reduced A. alternata-induced NaF6'H1 transcripts and scopoletin. Thus, it is concluded that JA signalling is activated in N. attenuata leaves after infection, which subsequently regulates scopoletin biosynthesis for the defence against A. alternata partly through MYC2, and higher levels of scopoletin accumulated in young leaves account for their strong resistance.
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http://dx.doi.org/10.1093/jxb/eru203DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4112635PMC
August 2014

Clinical application of long-term Palindrome catheter in hemodialysis patients.

Iran J Kidney Dis 2014 Mar;8(2):123-9

Department of Nephrology, Navy General Hospital, Beijing, China.

Introduction: This study was conducted to compare catheter function, dialysis adequacy, and dialysis-related complications among patients receiving long-term dialysis via Palindrome catheter, Permcath catheter, and arteriovenous fistula (AVF) as vascular access.

Materials And Methods: Forty-three patients undergoing dialysis with Permcath catheter, 49 with Palindrome catheter, and 56 with AVF were recruited. Urea clearance (KT/V), urea reduction rate, and the highest blood flow at the arteriovenous junction during dialysis were determined during the dialysis sessions. Catheter-related infection, catheter-associated thrombosis, and annual patency rate were also evaluated.

Results: In patients using Permcath catheter for dialysis, the incidence of secondary renal injury (metabolic diseases, hypertension, and ischemic kidney diseases) was 73.1%, which was significantly higher than that in patients with AVF (51.5%; P = .001). In the Palindrome group, maximum blood flow, KT/V, urea reduction rate, and annual patency rate were significantly higher than those in the Permcath group, and the incidence of access-related infection was significantly higher than that in the AVF group. In the Palindrome group, the prevalence of thromboembolism was 30.6%, which was significantly lower than that in the Permcath group (46.5%), but higher than that in the AVF group (5.4%).

Conclusions: For dialysis patients, Palindrome catheter was superior to Permcath catheter and comparable with the AVF in terms of the maximum blood flow, dialysis adequacy, and annual patency rate. Dialysis with Palindrome catheter has a high infection rate and a high incidence of thromboembolism as in the dialysis with Permcath catheter.
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March 2014

A novel splice-site mutation of ATP2A2 gene in a Chinese family with Darier disease.

Arch Dermatol Res 2010 Dec 21;302(10):769-72. Epub 2010 Sep 21.

Department of Dermatology, The Second Hospital of Xi'an Jiaotong University, 157 Xi Wu Road, Xi'an, 710004 Shaanxi, People's Republic of China.

Darier disease (DD; OMIM 124200) is a rare, autosomal dominant hereditary skin disorder characterized by abnormal keratinization and acantholysis. The causes of DD are defects in the ATP2A2 gene, which encodes the sarco/endoplasmic reticulum Ca(2+) ATPase isoform 2 (SERCA2). The aim of this study was to report a novel splice-site mutation and to examine the relative quantity expression of ATP2A2 gene in a Chinese family with DD. Polymerase chain reaction (PCR) was carried out to amplify the exons and flanking intron boundaries of the ATP2A2 gene followed by direct sequencing. A novel splice-site mutation (IVS20-6T>A) was found in the family, which was confirmed by creating a novel HinfI (NEB Inc) recognition site and RT-PCR. Real-time quantitative PCR showed approximately 53 and 52% reduction of ATP2A2 expression of the proband and his father, respectively. The results support the proposition that haploinsufficiency is a common mechanism for the dominant inheritance of DD.
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http://dx.doi.org/10.1007/s00403-010-1081-0DOI Listing
December 2010