Publications by authors named "Junfeng Wu"

86 Publications

Self-powered ultraviolet photodetector based on an n-ZnO:Ga microwire/p-Si heterojunction with the performance enhanced by a pyro-phototronic effect.

Opt Express 2021 Sep;29(19):30244-30258

In the present study, a heterojunction made of an individual ZnO microwire via Ga incorporation (ZnO:Ga MW) with a p-Si substrate was constructed to develop a self-powered ultraviolet photodetector. When operated under an illumination of 370 nm light with a power density of ∼ 0.5 mW/cm, the device exhibited an excellent responsivity of 0.185 A/W, a large detectivity of 1.75×10 Jones, and excellent stability and repeatability. The device also exhibited a high on/off photocurrent ratio up to 10, and a short rising and falling time of 499/412 μs. By integrating the pyro-phototronic effect, the maximum responsivity and detectivity increased significantly to 0.25 A/W and 2.30×10 Jones, respectively. The response/recovery time was drastically reduced to 79/132 μs without an external power source. In addition, the effects of light wavelength, power density, and bias voltage on the photocurrent response mediated by the pyro-phototronic effect were systematically characterized and discussed. Our work not only provides an easy yet efficient procedure for constructing a self-powered ultraviolet photodetector but also broadens the application prospects for developing individual wire optoelectronic devices based on the photovoltaic-pyro-phototronic effect.
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http://dx.doi.org/10.1364/OE.439587DOI Listing
September 2021

Grafting and Depositing Lithium Polysulfides on Cathodes for Cycling Stability of Lithium-Sulfur Batteries.

ACS Appl Mater Interfaces 2021 Sep 19;13(34):40685-40694. Epub 2021 Aug 19.

Institute of New Energy Material Chemistry, School of Materials Science and Engineering, Nankai University, Tianjin 300350, China.

Based on dissolution/deposition chemistry, together with multielectron redox reactions, lithium-sulfur (Li-S) batteries have been demonstrated as a promising energy storage system. However, the diffusion of soluble lithium polysulfide intermediates (LiPSs) to bulk electrolyte results in the fast capacity fade of a Li-S cell. How to confine the LiPSs within the cathode while retaining high reversible capacity remains a huge challenge. In this work, -bromophthalimide, an organic molecule with an aromatic heterocyclic ring and a reactive halogen bond, is introduced as an electrolyte additive to conquer the excessive dissolution and diffusion of LiPSs by in situ formation of an organopolysulfide deposition layer. This electrochemically active layer not only maintains the internal sulfur conversion but also prevents LiPSs from diffusing into the electrolyte bulk, thereby improving the cycling and rate performance of Li-S batteries. This study provides a feasible strategy for regulating the reaction region and path for high-performance Li-S batteries.
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http://dx.doi.org/10.1021/acsami.1c11904DOI Listing
September 2021

The chicken pan-genome reveals gene content variation and a promoter region deletion in IGF2BP1 affecting body size.

Mol Biol Evol 2021 Jul 30. Epub 2021 Jul 30.

College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.

Domestication and breeding have reshaped the genomic architecture of chicken, but the retention and loss of genomic elements during these evolutionary processes remain unclear. We present the first chicken pan-genome constructed using 664 individuals, which identified an additional ∼66.5 Mb sequences that are absent from the reference genome (GRCg6a). The constructed pan-genome encoded 20,491 predicated protein-coding genes, of which higher expression level are observed in conserved genes relative to dispensable genes. Presence/absence variation (PAV) analyses demonstrated that gene PAV in chicken was shaped by selection, genetic drift, and hybridization. PAV-based GWAS identified numerous candidate mutations related to growth, carcass composition, meat quality, or physiological traits. Among them, a deletion in the promoter region of IGF2BP1 affecting chicken body size is reported, which is supported by functional studies and extra samples. This is the first time to report the causal variant of chicken body size QTL located at chromosome 27 which was repeatedly reported. Therefore, the chicken pan-genome is a useful resource for biological discovery and breeding. It improves our understanding of chicken genome diversity and provides materials to unveil the evolution history of chicken domestication.
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http://dx.doi.org/10.1093/molbev/msab231DOI Listing
July 2021

Quantitative observation of monochromatic X-rays emitted from implosion hotspot in high spatial resolution in inertial confinement fusion.

Sci Rep 2021 Jul 14;11(1):14492. Epub 2021 Jul 14.

Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang, 621900, China.

In inertial confinement fusion, quantitative and high-spatial resolution ([Formula: see text]m) measurements of the X-rays self-emitted by the hotspot are critical for studying the physical processes of the implosion stagnation stage. Herein, the 8 ± 0.39-keV monochromatic X-ray distribution from the entire hotspot is quantitatively observed in 5-[Formula: see text]m spatial resolution using a Kirkpatrick-Baez microscope, with impacts from the responses of the diagnosis system removed, for the first time, in implosion experiments at the 100 kJ laser facility in China. Two-dimensional calculations along with 2.5% P2 drive asymmetry and 0.3 ablator self-emission are congruent with the experimental results, especially for the photon number distribution, hotspot profile, and neutron yield. Theoretical calculations enabled a better understanding of the experimental results. Furthermore, the origins of the 17.81% contour profile of the deuterium-deuterium hotspot and the accurate Gaussian source approximation of the core emission area in the implosion capsule are clarified in detail. This work is significant for quantitatively exploring the physical conditions of the hotspot and updating the theoretical model of capsule implosion.
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http://dx.doi.org/10.1038/s41598-021-93482-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8280192PMC
July 2021

Enzymatic/Magnetic Hybrid Micromotors for Synergistic Anticancer Therapy.

ACS Appl Mater Interfaces 2021 Jul 2;13(27):31514-31526. Epub 2021 Jul 2.

State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China.

Micro/nanomotors (MNMs), which propel by transforming various forms of energy into kinetic energy, have emerged as promising therapeutic nanosystems in biomedical applications. However, most MNMs used for anticancer treatment are only powered by one engine or provide a single therapeutic strategy. Although double-engined micromotors for synergistic anticancer therapy can achieve more flexible movement and efficient treatment efficacy, their design remains challenging. In this study, we used a facile preparation method to develop enzymatic/magnetic micromotors for synergetic cancer treatment via chemotherapy and starvation therapy (ST), and the size of micromotors can be easily regulated during the synthetic process. The enzymatic reaction of glucose oxidase, which served as the chemical engine, led to self-propulsion using glucose as a fuel and ST via a reduction in the energy available to cancer cells. Moreover, the incorporation of FeO nanoparticles as a magnetic engine enhanced the kinetic power and provided control over the direction of movement. Inherent pH-responsive drug release behavior was observed owing to the acidic decomposition of drug carriers in the intracellular microenvironment of cancer cells. This system displayed enhanced anticancer efficacy owing to the synergetic therapeutic strategies and increased cellular uptake in a targeted area because of the improved motion behavior provided by the double engines. Therefore, the demonstrated micromotors are promising candidates for anticancer biomedical microsystems.
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http://dx.doi.org/10.1021/acsami.1c07593DOI Listing
July 2021

Probiotics: their action against pathogens can be turned around.

Sci Rep 2021 06 24;11(1):13247. Epub 2021 Jun 24.

Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.

Probiotics when applied in complex evolving (micro-)ecosystems, might be selectively beneficial or detrimental to pathogens when their prophylactic efficacies are prone to ambient interactions. Here, we document a counter-intuitive phenomenon that probiotic-treated zebrafish (Danio rerio) were respectively healthy at higher but succumbed at lower level of challenge with a pathogenic Vibrio isolate. This was confirmed by prominent dissimilarities in fish survival and histology. Based upon the profiling of the zebrafish microbiome, and the probiotic and the pathogen shared gene orthogroups (genetic niche overlaps in genomes), this consequently might have modified the probiotic metabolome as well as the virulence of the pathogen. Although it did not reshuffle the architecture of the commensal microbiome of the vertebrate host, it might have altered the probiotic-pathogen inter-genus and intra-species communications. Such in-depth analyses are needed to avoid counteractive phenomena of probiotics and to optimise their efficacies to magnify human and animal well-being. Moreover, such studies will be valuable to improve the relevant guidelines published by organisations such as FAO, OIE and WHO.
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http://dx.doi.org/10.1038/s41598-021-91542-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225825PMC
June 2021

Small Low-Contrast Target Detection: Data-Driven Spatiotemporal Feature Fusion and Implementation.

IEEE Trans Cybern 2021 May 24;PP. Epub 2021 May 24.

Detecting small low-contrast targets in the airspace is an essential and challenging task. This article proposes a simple and effective data-driven support vector machine (SVM)-based spatiotemporal feature fusion detection method for small low-contrast targets. We design a novel pixel-level feature, called a spatiotemporal profile, to depict the discontinuity of each pixel in the spatial and temporal domains The spatiotemporal profile is a local patch of the spatiotemporal feature maps concatenated by the spatial feature maps and temporal feature maps in channelwise, which are generated by the morphological black-hat filter and a ghost-free dark-focusing frame difference methods, respectively. Instead of the handcrafted feature fusion mechanisms in previous works, we use the labeled spatiotemporal profiles to train an SVM classifier to learn the spatiotemporal feature fusion mechanism automatically. To speed up detection for high-resolution videos, the serial SVM classification process on central processing units (CPUs) is reformed as parallel convolution operations on graphics processing unit (GPUs), which exhibits over 1000+ times speedup in our real experiments. Finally, blob analysis is applied to generate final detection results. Elaborate experiments are conducted, and experimental results demonstrate that the proposed method performs better than 12 baseline methods for the small low-contrast target detection. The field tests manifest that the parallel implementation of the proposed method can realize real-time detection at 15.3 FPS for videos at a resolution of 2048x1536 and the maximum detection distance can reach 1 km for drones in sunny weather.
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http://dx.doi.org/10.1109/TCYB.2021.3072311DOI Listing
May 2021

The safety and efficacy of mini-percutaneous nephrolithotomy retrograde intrarenal surgery for treatment of renal lithiasis in pelvic ectopic kidney: an exploratory pilot study.

Authors:
Junfeng Wu Jun Shen

Transl Androl Urol 2021 Apr;10(4):1734-1742

Department of Urology, Qiandongnan People's Hospital Affiliated to Guizhou Medical University, Kaili, China.

Background: To compare the safety and efficacy of mini-percutaneous nephrolithotomy (PCNL) and retrograde intrarenal surgery (RIRS) for the treatment of renal lithiasis in patients with pelvic ectopic kidney.

Methods: From January 2015 to October 2017, mini-PCNL and RIRS were performed in ten patients diagnosed with lithiasis in pelvic ectopic kidneys, including three cases under laparoscopy-assisted mini-PCNL. Patient demographics and perioperative characteristics (age, gender, BMI, side of pelvic kidney, stone size, stone number, stone location, special medical history, and ASA physical status classification), and operative and post-operative related details (operation time, hospital stay, blood loss, VAS, analgesic requirement, complications, and stone free outcome) were reviewed.

Results: Although the mean operation time of mini-PCNL (71.3 min) was shorter than RIRS (85.3 min), the mean operation time of laparoscopy assisted mini-PCNL (92 min) was longer than patients without laparoscopy-assisted mini-PCNL (55.8 min). However, the use of mini-PCNL allowed for larger lithiasis to be dealt with (1.9 cm in laparoscopy assisted mini-PCNL and 2.4 cm in mini-PCNL without laparoscopy-assist) compared with RIRS (1.2 cm). In addition, although the mean hospital-stay time, blood loss, and analgesic requirement of patients undergoing RIRS were less than those receiving mini-PCNL, the success rate of RIRS was only 50% (3/6) in comparison to 100% (7/7) for mini-PCNL. Except for pain and urinary tract infection after the operation, there were no significant intraoperative and postoperative complications, and no residual lithiasis were seen in any patient.

Conclusions: Although RIRS was less time-consuming and invasive, mini-PCNL can deal with the bigger lithiasis and more complex situations with a higher success rate. Both mini-PCNL and RIRS are feasible and safe treatments for pelvic ectopic kidney lithiasis with each carrying unique advantages. Hence in practice, an appropriate individualized treatment should be selected depending on patient characteristics.
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http://dx.doi.org/10.21037/tau-21-77DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8100851PMC
April 2021

DHCR24 Knock-Down Induced Tau Hyperphosphorylation at Thr181, Ser199, Thr231, Ser262, Ser396 Epitopes and Inhibition of Autophagy by Overactivation of GSK3β/mTOR Signaling.

Front Aging Neurosci 2021 21;13:513605. Epub 2021 Apr 21.

Department of Neurology, Jinshan Hospital, Fudan University, Shanghai, China.

Accumulating evidences supported that knock-down of DHCR24 is linked to the pathological risk factors of AD, suggesting a potential role of DHCR24 in AD pathogenesis. However, the molecular mechanism link between DHCR24 and tauopathy remains unknown. Here, in order to elucidate the relationship between DHCR24 and tauopathy, we will focus on the effect of DHCR24 on the tau hyperphosphorylation at some toxic sites. In present study, we found that DHCR24 knock-down significantly lead to the hyperphosphorylation of tau sites at Thr181, Ser199, Thr231, Ser262, Ser396. Moreover, DHCR24 knock-down also increase the accumulation of p62 protein, simultaneously decreased the ratio of LC3-II/LC3-I and the number of autophagosome compared to the control groups, suggesting the inhibition of autophagy activity. In contrast, DHCR24 knock-in obviously abolished the effect of DHCR24 knock-down on tau hyperphosphrylation and autophagy. In addition, to elucidate the association between DHCR24 and tauopathy, we further showed that the level of plasma membrane cholesterol, lipid raft-anchored protein caveolin-1, and concomitantly total I class PI3-K (p110α), phospho-Akt (Thr308 and Ser473) were significantly decreased, resulting in the disruption of lipid raft/caveola and inhibition of PI3-K/Akt signaling in silencing DHCR24 SH-SY5Y cells compared to control groups. At the same time, DHCR24 knock-down simultaneously decreased the level of phosphorylated GSK3β at Ser9 (inactive form) and increased the level of phosphorylated mTOR at Ser2448 (active form), leading to overactivation of GSK3β and mTOR signaling. On the contrary, DHCR24 knock-in largely increased the level of membrane cholesterol and caveolin-1, suggesting the enhancement of lipid raft/caveola. And synchronously DHCR24 knock-in also abolished the effect of DHCR24 knock-down on the inhibition of PI3-K/Akt signaling as well as the overactivation of GSK3β and mTOR signaling. Collectively, our data strongly supported DHCR24 knock-down lead to tau hyperphosphorylation and the inhibition of autophagy by a lipid raft-dependent PI3-K/Akt-mediated GSK3β and mTOR signaling. Taking together, our results firstly demonstrated that the decrease of plasma membrane cholesterol mediated by DHCR24 deficiency might contribute to the tauopathy in AD and other tauopathies.
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http://dx.doi.org/10.3389/fnagi.2021.513605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8098657PMC
April 2021

Sustained delivery of MMP-9 siRNA via thermosensitive hydrogel accelerates diabetic wound healing.

J Nanobiotechnology 2021 May 5;19(1):130. Epub 2021 May 5.

Department of Endocrinology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120, P. R. China.

Excessive expression of matrix metalloproteinase 9 (MMP-9) impedes healing of diabetic chronic wounds, thus wound dressing that could effectively inhibit the expression of MMP-9 offers significant clinical translation for diabetic wound healing. Herein, a hybrid hydrogel dressing was developed for localized and sustained delivery of MMP-9 siRNA (siMMP-9). siMMP-9 was complexed with Gly-TETA (GT), the GT/siMMP9 complex was then loaded into a thermosensitive hydrogel based on Pluronic F-127 (PF) and methylcellulose (MC). In vitro, this hybrid hydrogel dressing exhibited negligible cytotoxicity, prolonged the release of GT/siMMP-9 for up to 7 days, and significantly reduced MMP-9 expression. In vivo assessment in diabetic rats demonstrated that hydrogel provided localized and sustained delivery via the thermosensitive controlled release of entrapped GT/siMMP-9 into wound tissues for 7 days, resulting in dramatic MMP-9 silencing which significantly improved diabetic wound closure. This hybrid hydrogel dressing exhibited excellent biocompatibility, with no observed systemic toxicity in rats. Taken together, the hybrid hydrogel dressing may constitute an effective and biocompatible means of enhancing diabetic wound healing through effective silencing of the MMP-9 gene, and this hydrogel delivery system also offers a platform for in vivo delivery of siRNA for the treatment of other diseases.
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http://dx.doi.org/10.1186/s12951-021-00869-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8097905PMC
May 2021

Words that hurt: Leaders' anti-Asian communication and employee outcomes.

Authors:
Sora Jun Junfeng Wu

J Appl Psychol 2021 Feb;106(2):169-184

Naveen Jindal School of Management, University of Texas at Dallas.

With the outbreak of COVID-19, there have been growing reports of racial harassment targeting Asian Americans. We study one such manifestation of racial harassment that Asian employees may face in the workplace: Leaders' use of stigmatizing labels for COVID-19 such as the "Chinese Virus" and "Kung Flu." Integrating organizational justice theories with research on racial harassment in the workplace, we theorize that leaders' use of stigmatizing COVID-19 labels reduces employees' perceptions of interpersonal justice, which subsequently impact employees' emotional exhaustion and work engagement. We further theorize that while such effects will be stronger among Asian employees who experience both moral anger and reduced public collective self-esteem, that the effects will also be present among non-Asian employees who experience moral anger. Using one survey (Study 1) and one experiment (Study 2), we find support for our predictions. We find that leaders' use of stigmatizing language to depict COVID-19 leads to deleterious workplace experiences for employees, and especially for Asian employees. The current research thus deepens our understanding of the relatively understudied work experiences of Asian Americans and brings to light the underlying psychological mechanisms linking racial harassment and employee work outcomes for both targeted employees and employees not targeted. (PsycInfo Database Record (c) 2021 APA, all rights reserved).
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http://dx.doi.org/10.1037/apl0000873DOI Listing
February 2021

Laser Shock Peening of SiCp/2009Al Composites: Microstructural Evolution, Residual Stress and Fatigue Behavior.

Materials (Basel) 2021 Feb 26;14(5). Epub 2021 Feb 26.

School of Mechanical Engineering and Automation, Beihang University, Beijing 100191, China.

SiC particle reinforced aluminum alloy has a wide application in the aerospace industries. In this study, laser shock peening (LSP), an advanced surface modification technique, was employed for SiCp/2009Al composite to reveal its microstructure, microhardness and residual stress evolution. After peening, high densities of dislocations were induced in the aluminum substrate, and stacking faults were introduced into the SiC particle. The microhardness was increased from 155-170 HV to 170-185 HV, with an affected depth of more than 1.5 mm. Compressive residual stresses of more than 200 MPa were introduced. The three-point bending fatigue of the base material, laser peened and milled after laser peened specimens with artificial crack notch fabricated by a femtosecond laser was investigated. The average fatigue lives of laser peened and milled after laser peened specimens were increased by up to 10.60 and 2.66 times, compared with the base material. This combined fundamental and application-based research seeks to comprehensively explore the applicability of LSP on metal matrix composite.
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http://dx.doi.org/10.3390/ma14051082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7956191PMC
February 2021

Preparation of β-cyclodextrin/graphene oxide and its adsorption properties for methylene blue.

Colloids Surf B Biointerfaces 2021 Apr 3;200:111605. Epub 2021 Feb 3.

College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 100049, China.

Graphene oxide (GO) and GO-based materials have shown excellent adsorption properties because of bounteous structure and rich oxygen functional groups. Many studies have shown that GO are utilized as adsorbents to remove organic dyes from wastewater. GO was prepared by modified Hummers method using graphite powder as raw material. On this basis, β-cyclodextrin/graphene oxide composite (β-CD/GO) was prepared by modifying graphene oxide via β-cyclodextrin(β-CD) crosslinking method. GO and β-CD were characterized by Fourier transform infrared spectroscopy (FT-IR), energy-dispersive X-ray (XRD), scanning electron (SEM) and thermogravimetric analysis (TGA). Their adsorbents properties have been studied with methylene blue (MB) as adsorbate. The factors affecting the study include the temperature, adsorption time, amount of adsorbent and system pH value. Adsorption isotherm and kinetics of the adsorption process are systematically analyzed. The results show that β-CD/GO has a different adsorption capacity from GO under the same adsorption factors. Under the optimized conditions (the reaction temperature is 70 °C, the reaction time is 60 min and the concentration of adsorbent is 0.04 g/L), the removal efficiency of β-CD/GO is 20% higher than that of GO from 70% to 90%. The maximum adsorption capacity of β-CD/GO is 76.4 mg/g. β-CD/GO can be effectively regenerated by elution with absolute alcohol. In these tests, β-CD/GO was suggested to be more efficient than GO in the removal of organic dyes.
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http://dx.doi.org/10.1016/j.colsurfb.2021.111605DOI Listing
April 2021

Study on Evaluation Model of Emergency Rescue Capability of Chemical Accidents Based on PCA-BP.

Comput Intell Neurosci 2021 13;2021:8869608. Epub 2021 Jan 13.

College of Ocean Science and Engineering, Shanghai Maritime University, Shanghai 201306, China.

The emergency management of chemical accidents plays an important role in preventing the expansion of chemical accidents. In recent years, the evaluation and research of emergency management of chemical accidents has attracted the attention of many scholars. However, as an important part of emergency management, the professional rescue team of chemicals has few evaluation models for their capabilities. In this study, an emergency rescue capability assessment model based on the PCA-BP neural network is proposed. Firstly, the construction status of 11 emergency rescue teams for chemical accidents in Shanghai is analyzed, and an index system for evaluating the capabilities of emergency rescue teams for chemicals is established. Secondly, the principal component analysis (PCA) is used to perform dimension reduction and indicators' weight acquisition on the original index system to achieve an effective evaluation of the capabilities of 11 rescue teams. Finally, the indicators after dimensionality reduction are used as the input neurons of the backpropagation (BP) neural network, the characteristic data of eight rescue teams are used as the training set, and the comprehensive scores of three rescue teams are used for verifying the generalization ability of the evaluation model. The result shows that the proposed evaluation model based on the PCA-BP neural network can effectively evaluate the rescue capability of the emergency rescue teams for chemical accidents and provide a new idea for emergency rescue capability assessment.
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http://dx.doi.org/10.1155/2021/8869608DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822681PMC
July 2021

Preparation of nitrogen self-doped hierarchical porous carbon with rapid-freezing support for cooperative pollutant adsorption and catalytic oxidation of persulfate.

Sci Total Environ 2021 Jan 8;752:142282. Epub 2020 Sep 8.

Shanghai Key Laboratory of Materials Protection and Advanced Materials in Electric Power, Shanghai University of Electric Power, Shanghai 200090, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200090, PR China.

Herein, we report a method to synthesize nitrogen self-doped hierarchical porous carbon materials derived from chitosan. This method uses potassium hydroxide (KOH) activation and rapid-freezing technology. The catalyst (CA-900Q 1-1) obtained after rapid-freezing and KOH activation treatment show excellent persulfate activation ability. It can remove 20 mg bisphenol A (BPA) within 10 min better than traditional metal oxidate and nanomaterials. In the aquatic environment, CA-900Q 1-1 has a high resistance to inorganic anions. CA-900Q 1-1, possessing a high proportion of graphitic nitrogen, provides a sufficient number of active sites for persulfate activation. In addition, the catalyst yielded sizeable specific surface areas (SSAs) (1756.1 m/g) and a hierarchical pore structure, which helps to improve the mass transfer in the carbon framework. The efficient adsorption of pollutants by the catalyst shortens the time required for target organic molecules to migrate to the catalyst surface and hierarchical pore structure. Furthermore, the catalyst has excellent electrical conductivity (R = 1.73 Ω), which enables pollutants adsorbed on the catalyst surface to transfer electrons to the persulfate through the N-doped sp-hybrid carbon network faster.
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http://dx.doi.org/10.1016/j.scitotenv.2020.142282DOI Listing
January 2021

Genome-wide association study reveals the genetic determinism of growth traits in a Gushi-Anka F chicken population.

Heredity (Edinb) 2021 Feb 28;126(2):293-307. Epub 2020 Sep 28.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China.

Chicken growth traits are economically important, but the relevant genetic mechanisms have not yet been elucidated. Herein, we performed a genome-wide association study to identify the variants associated with growth traits. In total, 860 chickens from a Gushi-Anka F resource population were phenotyped for 68 growth and carcass traits, and 768 samples were genotyped based on the genotyping-by-sequencing (GBS) method. Finally, 734 chickens and 321,314 SNPs remained after quality control and removal of the sex chromosomes, and these data were used to carry out a GWAS analysis. A total of 470 significant single-nucleotide polymorphisms (SNPs) for 43 of the 68 traits were detected and mapped on chromosomes (Chr) 1-6, -9, -10, -16, -18, -23, and -27. Of these, the significant SNPs in Chr1, -4, and -27 were found to be associated with more than 10 traits. Multiple traits shared significant SNPs, indicating that the same mutation in the region might have a large effect on multiple growth or carcass traits. Haplotype analysis revealed that SNPs within the candidate region of Chr1 presented a mosaic pattern. The significant SNPs and pathway enrichment analysis revealed that the MLNR, MED4, CAB39L, LDB2, and IGF2BP1 genes could be putative candidate genes for growth and carcass traits. The findings of this study improve our understanding of the genetic mechanisms regulating chicken growth and carcass traits and provide a theoretical basis for chicken breeding programs.
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http://dx.doi.org/10.1038/s41437-020-00365-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026619PMC
February 2021

UV-assisted nitrogen-doped reduced graphene oxide/FeO composite activated peroxodisulfate degradation of norfloxacin.

Environ Technol 2020 Jun 17:1-12. Epub 2020 Jun 17.

Henan Key Laboratory of Water Pollution Control and Rehabilitation Technology, Henan University of Urban Construction, Pingdingshan, People's Republic of China.

We reported the preparation of NGO-FeO by simple hydrothermal-co-precipitation. The catalysts were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). It was demonstrated that norfloxacin (NOR) could be effectively degraded by the UV/NGO-FeO/PDS system. The degradation efficiency reached 100% within 13 min (the concentration of NOR and were 100 mg L and 1 mM, respectively; m(NGO-FeO): m(PDS) = 4: 1; pH: 3.0). In addition, NGO-FeO showed stable catalytic activity in recycling. The analysis found that the in-situ generated ·OH was the main active free radicals but also participated in the NOR degradation. Based on the identified intermediates, the NOR degradation pathways were proposed with UV/NGO-FeO/PDS system.
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http://dx.doi.org/10.1080/09593330.2020.1779353DOI Listing
June 2020

Visible light-assisted NGO-FeO composite activated peroxydisulfate for degradation of oxytetracycline.

Water Sci Technol 2020 Feb;81(4):813-823

Henan Province Key Laboratory of Water Pollution Control and Rehabilitation Technology, Henan University of Urban Construction, Pingdingshan, 467036, China E-mail:

A nitrogen-doped reduced graphene oxide/FeO composite (NGO-FeO) was prepared through the simplified hydrothermal and deposition-precipitation method and characterized by X-ray diffraction, scanning electron microscopy and Fourier transform infrared spectroscopy. The degradation efficiency of oxytetracycline (OTC) by NGO-FeO activated peroxodisulfate (PDS) under visible light irradiation was studied. The degradation efficiency reached 100% within 32.5 min (the initial OTC concentration 50 mg L and PDS 1 mM; [NGO-FeO]:[ PDS] = 4:1; pH = 3.0). No apparent decrease in degradation efficiency was observed after five cycles. SO · and ·OH were the main active oxides for OTC degradation in this system. Moreover, four degradation pathways were proposed, namely hydroxylation, dehydration, decarbonylation and demethylation according to the analysis results of high-performance liquid chromatography mass spectrometry.
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http://dx.doi.org/10.2166/wst.2020.164DOI Listing
February 2020

Rapid cultivation and stability of autotrophic nitrifying granular sludge.

Water Sci Technol 2020 Jan;81(2):309-320

School of Architectural and Surveying & Mapping Engineering, Jiangxi University of Science and Technology, Hongqi Ave. 86, Ganzhou 341000, Jiangxi, China E-mail:

Autotrophic nitrifying granular sludge (ANGS) was cultivated by gradually decreasing the influent organics and adding exogenous nitrifying bacteria. Under the strategy, ANGS was domesticated within 36 days. Stability of the seed heterotrophic granules decreased significantly during conversion of organic wastewater to inorganic ammonia wastewater. Obvious granular breakage was observed during these days. However, the granular debris still had good settlement performance. With microbes gradually acclimated to the new environment, the debris provided a large number of carriers for the attached growth of the exogenous nitrifying bacteria, and they replaced the heterotrophic bacteria and became the dominant species. The domesticated ANGS showed good nitrification performance during the 37th to the 183rd day (ammonia nitrogen load between 0.28 and 0.29 kg/m · d). The removal rate of ammonia nitrogen was usually more than 95%, and nitrite accumulation rate was always larger than 50%. However, nitrification ability was gradually lost with the increase of the ammonia nitrogen load (0.3-0.64 kg/m · d) from the 184th day, and it almost approached the influent ammonia nitrogen at the 269th day. Interestingly, good structure stability of the ANGS was maintained during long-term operation, and the ANGS became smoother and denser at the end of the experiment.
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http://dx.doi.org/10.2166/wst.2020.111DOI Listing
January 2020

Does manager servant leadership lead to follower serving behaviors? It depends on follower self-interest.

J Appl Psychol 2021 Jan 9;106(1):152-167. Epub 2020 Apr 9.

Department of Managerial Studies, University of Illinois at Chicago.

One distinguishing feature of servant leadership is the proposition that servant leaders develop followers who also engage in serving behaviors. Drawing upon social learning theory, we argue that follower dispositional self-interest is a boundary condition affecting the transference of manager servant leadership to follower engagement in serving behaviors, and that follower serving self-efficacy is the underlying psychological mechanism. In a laboratory experiment (Study 1), we manipulated manager servant leadership and found support for the hypothesis that the positive relationship between manager servant leadership and follower serving behaviors is significantly enhanced for participants high in self-interest. The serving behaviors of participants low in self-interest was not affected by the degree to which the manager practiced servant leadership. In a field study (Study 2) with a sample representing 10 diverse organizations in Singapore, we replicated the findings. In another laboratory experiment (Study 3), we demonstrated that follower serving self-efficacy mediated the interactional effect found in the first two studies, supporting the social learning account for the transference of manager servant leadership to follower serving behaviors. Taken together, converging results from these three studies demonstrate that servant leaders are capable of bringing out serving behaviors especially among followers with a strong focus on their own self-interest. (PsycInfo Database Record (c) 2021 APA, all rights reserved).
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http://dx.doi.org/10.1037/apl0000500DOI Listing
January 2021

Antibiotics promote abdominal fat accumulation in broilers.

Anim Sci J 2020 Jan-Dec;91(1):e13326

College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, China.

Antibiotics stimulate the growth of animals but result in drug residues and bacterial resistance. In this study, the negative effect of antibiotics on abdominal fat deposition was evaluated in broilers. The results showed that adding both chlortetracycline (50 g/1,000 kg) and tylosin (50 g/1,000 kg) significantly increased abdominal fat weight, abdominal fat percentage (p < .05), and triglyceride and cholesterol levels (p < .05) in blood. Also, both products synchronously stimulated intestinal absorption and synthesis of liver fat. The expression levels of the peroxisome proliferator-activated receptor γ (PPARγ), diacylgycerol acyltransferase 2 (DGAT2), lipoprotein lipase (LPL), and fatty acid-binding protein (FABP4) genes in abdominal fat tissue significantly increased (p < .05 or 0.01) when antibiotics were added to the feed. However, no significant difference was found in expression of the fatty acid synthesis (FAS) or acetyl CoA carboxylase (ACC) genes. Further in vitro study results revealed that antibiotics had no effect on fat content or the related gene expression levels in preadipocytes. In summary, the antibiotics induced fat deposition in adipose tissues by activating extracellular absorption of fatty acids from intestinal absorption and synthesis of liver fat. However, it shows no direct regulation by adipose tissue.
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http://dx.doi.org/10.1111/asj.13326DOI Listing
September 2020

Hyperbranched cationic polysaccharide derivatives for efficient siRNA delivery and diabetic wound healing enhancement.

Int J Biol Macromol 2020 Jul 18;154:855-865. Epub 2020 Mar 18.

Department of Endocrinology, Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, PR China. Electronic address:

Gene vectors are important for successful siRNA delivery. Four types of hyperbranched cationic polysaccharide derivatives (HCP) were synthesized by conjuncting 1,2-ethylenediamine (EDA) and diethylenetriamine (DETA) with glycogen or amylopectin respectively and named as G-EDA, G-DETA, A-EDA and A-DETA. The efficiency and safety of these HCPs to deliver siRNA were explored in vitro and in vivo. Our results showed that HCPs could form complexes with siRNA. All HCP/siRNA exhibited negligible cytotoxicity. Compared with A-EDA and A-DETA, G-EDA and G-DETA could carry much more siRNA into cells and then escape from endosomes. The delivery of MMP-9 siRNA (siMMP-9) by G-EDA and G-DETA significantly inhibited MMP-9 in HaCaT cells. Wound models in diabetic rats demonstrated that treatment of G-EDA/siMMP-9 could potently knock down MMP-9 of skin wound tissues and then enhanced wound healing. In summary, this study provided an effective and safe approach for siRNA delivery in vitro and in vivo.
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http://dx.doi.org/10.1016/j.ijbiomac.2020.03.164DOI Listing
July 2020

Low Dose CT Image Reconstruction Based on Structure Tensor Total Variation Using Accelerated Fast Iterative Shrinkage Thresholding Algorithm.

Sensors (Basel) 2020 Mar 16;20(6). Epub 2020 Mar 16.

Equipment Management and Unmanned Aerial Vehicle Engineering College, Air Force Engineering University, Xi'an 710051, China.

Low dose computed tomography (CT) has drawn much attention in the medical imaging field because of its ability to reduce the radiation dose. Recently, statistical iterative reconstruction (SIR) with total variation (TV) penalty has been developed to low dose CT image reconstruction. Nevertheless, the TV penalty has the drawback of creating blocky effects in the reconstructed images. To overcome the limitations of TV, in this paper we firstly introduce the structure tensor total variation (STV) penalty into SIR framework for low dose CT image reconstruction. Then, an accelerated fast iterative shrinkage thresholding algorithm (AFISTA) is developed to minimize the objective function. The proposed AFISTA reconstruction algorithm was evaluated using numerical simulated low dose projection based on two CT images and realistic low dose projection data of a sheep lung CT perfusion. The experimental results demonstrated that our proposed STV-based algorithm outperform FBP and TV-based algorithm in terms of removing noise and restraining blocky effects.
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http://dx.doi.org/10.3390/s20061647DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146515PMC
March 2020

Abatacept is effective in Chinese patients with LRBA and CTLA4 deficiency.

Genes Dis 2021 Sep 12;8(5):662-668. Epub 2020 Mar 12.

Chongqing Key Laboratory of Child Infection and Immunity, Children's Hospital of Chongqing Medical University, Chongqing, 400015, PR China.

CTLA4 deficiency and LRBA deficiency are a group disorders of immune dysregulation that affect CTLA4 pathway. The patients mainly present with autoimmunity, antibody deficiency and recurrent infections. Here we reported three Chinese patients with and mutations. They all presented with chronic diarrhea, hypokalemia, organomegaly, recurrent infections, and hypogammaglobulinemia. Reduced Treg cells and increased percentage of circulating follicular helper T (cTfh) cells were revealed in these patients. Although steroid and immunoglobulin therapy were given, the enteropathy was persistent. Therefore, abatacept treatment was provided to these patients. They showed a marked improvement of enteropathy and gastrointestinal endoscopy showed alleviated inflammatory lesion and follicular hyperplasia. Furthermore, the frequency of cTfh cells was reduced after abatacept therapy. Taken together, targeted therapy with abatacept is a promising treatment modality for patients with LRBA and CTLA4 deficiency. The findings also suggest that the frequency of cTfh cells could serve as a marker for tracking disease activity and the response to abatacept therapy.
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http://dx.doi.org/10.1016/j.gendis.2020.03.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8278529PMC
September 2021

Nanoheterostructure Construction and DFT Study of Ni-Doped InO Nanocubes/WS Hexagon Nanosheets for Formaldehyde Sensing at Room Temperature.

ACS Appl Mater Interfaces 2020 Mar 2;12(10):11979-11989. Epub 2020 Mar 2.

College of Control Science and Engineering, China University of Petroleum (East China), Qingdao 266580, China.

A high-performance formaldehyde sensor based on nickel (Ni)-doped indium trioxide (InO)/tungsten disulfide (WS) nanocomposite was demonstrated. An epoxy substrate served as matrix of the Ni-InO/WS nanocomposite sensor. The material properties of self-assembled Ni-InO/WS nanoheterostructure were fully characterized and confirmed. The formaldehyde-sensing properties of the Ni-InO/WS composite were tested at 25 °C. Compared to the InO, WS, and their composite, the Ni-InO/WS sensor demonstrated significant improvement on the formaldehyde-sensing performance, including a low detection limit of 15 ppb, good selectivity, repeatability, fast detection rate, and a fair logarithmic function toward formaldehyde concentration. The dramatically enhanced sensing performance of Ni-InO/WS film sensor can be attributed to the Ni ion doping and synergistic interfacial incorporation of InO/WS heterojunction. The sensitive mechanism of the Ni-InO/WS film sensor toward formaldehyde is explored through density functional theory (DFT) simulation. This work verified that the synthesis of Ni-doped InO/WS nanofilm provides a new avenue to develop promising hybrids for formaldehyde sensing.
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http://dx.doi.org/10.1021/acsami.9b15200DOI Listing
March 2020

Evolution of microbial community during dry storage and recovery of aerobic granular sludge.

Heliyon 2019 Dec 12;5(12):e03023. Epub 2019 Dec 12.

School of Architectural and Surveying & Mapping Engineering, Jiangxi University of Science and Technology, Hongqi Ave. 86, Ganzhou, 341000, Jiangxi, China.

Aerobic granular sludge (AGS) was imbedded in agar and stored at 4 °C for 30 days, and then the stored granules were recovered in a sequencing batch reactor fed real wastewater within 11 days. Variations in microbial community compositions were investigated during dry storage and recovery of AGS, aiming to elucidate the mechanism of granular stability loss and recovery. The storage and recovery of AGS involved microbial community evolution. The dominant bacterial genera of the mature AGS were (relative abundance of 22.39%), (16.03%) and (11.17%), and those of the stored granules were (26.79%), (12.83%) and (5.69%), respectively. However, the dominant genera were (43.64%), (12.3.6%) and (11.47%) in the recovered AGS. Methanogens were always the dominant archaeal species in mature AGS (93.01%), stored granules (99.99%) and the recovered AGS (94.84%). Facultative anaerobes and anaerobes proliferated and dominated in the stored granules, and their metabolic activities gradually led to granular structure destruction and property deterioration. However, the stored granules served as carriers for the microbes originated from the real septic tank wastewater during recovery. They proliferated rapidly and secreted a large number of extracellular polymeric substances which helped to recover the granular structure in 11 days.
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http://dx.doi.org/10.1016/j.heliyon.2019.e03023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6926229PMC
December 2019

Inhibition of PRMT5 Attenuates Oxidative Stress-Induced Pyroptosis via Activation of the Nrf2/HO-1 Signal Pathway in a Mouse Model of Renal Ischemia-Reperfusion Injury.

Oxid Med Cell Longev 2019 25;2019:2345658. Epub 2019 Nov 25.

Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.

Background: Extensive evidence has demonstrated that oxidative stress, pyroptosis, and proinflammatory programmed cell death are related to renal ischemia/reperfusion (I/R) injury. However, the underlying mechanism remains to be illustrated. Protein arginine methylation transferase 5 (PRMT5), which mediates arginine methylation involved in the regulation of epigenetics, exhibits a variety of biological functions and essential roles in diseases. The present study investigated the role of PRMT5 in oxidative stress and pyroptosis induced by I/R injury in a mouse model and in a hypoxia/reoxygenation (H/R) model of HK-2 cells.

Methods: C57 mice were used as an animal model. All mice underwent right nephrectomy, and the left renal pedicles were either clamped or not. Renal I/R injury was induced by ligating the left renal pedicle for 30 min followed by reperfusion for 24 h. HK-2 cells were exposed to normal conditions or stimulation through H/R. EPZ015666(EPZ)-a selective potent chemical inhibitor-and small interfering RNA (siRNA) were administered to suppress the function and expression of PRMT5. The levels of urea nitrogen and creatinine in the serum and renal tissue injury were assessed. Immunohistochemistry, western blotting, and reverse transcription-polymerase chain reaction were used to evaluate pyroptosis-related proteins including nod-like receptor protein-3, ASC, caspase-1, caspase-11, GSDMD-N, and interleukin-1. Cell apoptosis and cell viability were detected through flow cytometry, and the levels of reactive oxygen species (ROS) and hydrogen peroxide (HO) were measured. Ki-67 was used to assess the proliferation of renal tubular epithelium. In addition, the activity of malondialdehyde and superoxide dismutase was determined.

Results: I/R or H/R induced an increase in the expression of PRMT5. Inhibition of PRMT5 by EPZ alleviated oxidative stress and I/R- or H/R-induced pyroptosis. In renal tissue, the application of EPZ promoted the proliferation of tubular epithelium. In addition, H/R-induced pyroptosis in HK-2 cells was dependent on oxidative stress in vitro. Administration of either EPZ or siRNA led to decreased expression of pyroptosis-related proteins. Inhibition of PRMT5 also attenuated the I/R- or H/R-induced oxidative stress in vivo and in HK-2 cells, respectively. It also resulted in a distinct decrease in the levels of malondialdehyde and HO, and an apparent increase in superoxide dismutase activity in mouse renal tissue. Moreover, it led to a significant decrease in the levels of ROS and HO in HK-2 cells. When activated, NF-E2-related factor/heme oxygenase-1 (Nrf2/HO-1)-a key regulator of various cytoprotective proteins that withstand oxidative damage-can decrease the generation of ROS. Nrf2/HO-1 was downregulated during I/R in tissues and H/R in HK-2 cells, and this effect was reversed by the PRMT5 inhibitor. Furthermore, the expressions of Nrf2 and HO-1 proteins were markedly upregulated by EPZ or siRNA against PRMT5.

Conclusion: PRMT5 is involved in ischemia- and hypoxia-induced oxidative stress and pyroptosis in vitro and in vivo. Inhibition of PRMT5 may ameliorate renal I/R injury by suppressing oxidative stress and pyroptosis via the activation of the Nrf2/HO-1 pathway, as well as promoting the proliferation of tubular epithelium. Therefore, PRMT5 may be a promising therapeutic target.
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http://dx.doi.org/10.1155/2019/2345658DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899313PMC
May 2020

Rapid domestication of autotrophic nitrifying granular sludge and its stability during long-term operation.

Environ Technol 2021 Jun 31;42(16):2587-2598. Epub 2019 Dec 31.

School of Architectural and Surveying & Mapping Engineering, Jiangxi University of Science and Technology, Ganzhou, People's Republic of China.

The nitrifying granular sludge process is a prospective technology for the efficient treatment of rare earth mine wastewater in southern Jiangxi, China. However, the long formation time of nitrifying granular sludge greatly restricted its application. In the present study, nitrifying granules were domesticated in a pilot-scale sequencing batch reactor by using heterotrophic granular sludge as carriers and adding exogenous nitrifying bacteria concentrate. According to variations of granular properties and ammonia removal, autotrophic nitrifying granules were successfully domesticated within 38 days using the strategy. It was found that the process involved secondary nucleation and microbial community evolution of the seed heterotrophic granules, and replaced most heterotrophic bacteria and became the dominant species with the largest relative abundance. During the subsequent 168 days of operation, the domesticated autotrophic nitrifying granules were stable, and their structures were denser than those of the inoculated granules. The ammonia nitrogen removal rate of the reactor was greater than 90% for a long period of time, and persistent partial nitrification was once achieved. However, severe fluctuation of influent ammonia nitrogen during the 120th to 206th day significantly inhibited the activity of nitrifying bacteria, which gradually led to the decrease of the ammonia nitrogen removal rate.
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http://dx.doi.org/10.1080/09593330.2019.1707881DOI Listing
June 2021

Naturally-occurring bacterial cellulose-hyperbranched cationic polysaccharide derivative/MMP-9 siRNA composite dressing for wound healing enhancement in diabetic rats.

Acta Biomater 2020 01 18;102:298-314. Epub 2019 Nov 18.

Department of Endocrinology, Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, PR China. Electronic address:

The anomalous high expression of matrix metalloproteinase 9 (MMP-9) is one important factor that impedes diabetic wound healing. Therefore, inhibition of MMP-9 expression in a diabetic wound could be a feasible method to promote wound healing. In this study, we studied the possibility of self-therapy using wound dressings that contain bacterial cellulose-hyperbranched cationic polysaccharide (BC-HCP) derivatives that encapsulate siRNA (BC-HCP/siMMP-9) and have controlled release properties. Herein, we used four HCPs (Gly-DMAPA, Gly-D4, Amyp-DMAPA, Amyp-D4) as gene carriers. Our results showed that all HCP derivatives were minimally toxic to cells in vitro, while the cationic properties of HCP could be used as a complexation agent for MMP-9 siRNA (siMMP-9). Upon exposure to bacterial cellulose (BC), the BC slowly released HCP/siMMP-9. The released siMMP-9 effectively reduced the gene expression and protein levels of MMP-9 in a human immortalized epithelial cell line (HaCAT) and in diabetic rat wounds. Inhibition of MMP-9 in the wounds of diabetic rats resulted in a significant enhancement of wound healing, suggesting that the BC-HCP/siMMP-9 composite dressing could be used as a safe and effective dressing to promote wound healing in diabetic rats. STATEMENT OF SIGNIFICANCE: In this work, we evaluated the possibility of using bacterial cellulose-hyperbranched cationic polysaccharide derivatives (BC-HCP) as a self-therapeutic wound dressing with siRNA encapsulated and controlled release properties. Our results showed that the BC-HCP/siMMP-9 composite dressing slowly released HCP/siMMP-9. The released siMMP-9 effectively reduced the gene expression and protein level of MMP-9 in human immortalized epithelial cell line and in the wound of diabetic rats. The BC-HCP/siMMP-9 composite dressing promoted diabetic wound healing by the unique nanostructure of BC and by releasing siMMP-9 for specific MMP-9 inhibition. Therefore, it could be used as a safe and effective dressing to promote wound healing in diabetic rats. This is the first evidence on the study of using BC as a dressing composite by encapsulating HCP/siRNA complexes for efficient RNAi gene silencing for better wound healing in diabetic rats.
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http://dx.doi.org/10.1016/j.actbio.2019.11.005DOI Listing
January 2020

Anti-inflammatory effects of doxepin hydrochloride against LPS-induced C6-glioma cell inflammatory reaction by PI3K-mediated Akt signaling.

J Biochem Mol Toxicol 2020 Feb 19;34(2):e22424. Epub 2019 Nov 19.

Department of Neurology, Jinshan Hospital Affiliated to Fudan University, Shanghai, China.

Recent studies have shown that tricyclic antidepressants (TCAs) may have anti-inflammatory and anticonvulsant effects in addition to its antidepressant effects. So far, the nonantidepressant effects of TCAs and their molecular pharmacological mechanisms remain completely unclear. Chronic inflammation in the brain parenchyma may be related to the pathogenesis and progression of various neurodegenerative diseases. As a common antidepressant and anti-insomnia drug, doxepin also may be a potential anti-inflammatory and anticonvulsant drug, so the study on the anti-inflammatory protective effect of doxepin and its molecular mechanism has become a very important issue in pharmacology and clinical medicine. Further elucidating the anti-inflammatory and neuroprotective effects of doxepin and its molecular mechanism may provide the important theoretical and clinical basis for the prevention and treatment of neurodegenerative disease. This study was designed to understand the glio-protective mechanism of doxepin against the inflammatory damage induced by lipopolysaccharide (LPS) exposure in C6-glioma cells. We found the treatment of C6-glioma cells with LPS results in deleterious effects, including the augmentation of inflammatory cytokine levels (tumor necrosis factor-α, interleukin-1β), and suppresses the Akt phosphorylation. Furthermore, our outcomes demonstrated that doxepin was able to suppress these effects induced by LPS, through activation of the phosphatidylinositol-3-kinase-mediated protein kinase B (Akt) pathway. To sum up, these results highlight the potential role of doxepin against neuroinflammatory-related disease in the brain.
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http://dx.doi.org/10.1002/jbt.22424DOI Listing
February 2020
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