Publications by authors named "Junfeng Liu"

364 Publications

Direct and Inverse Reduced-Form Models for Reciprocal Calculation of BC Emissions and Atmospheric Concentrations.

Environ Sci Technol 2021 Jul 23. Epub 2021 Jul 23.

College of Urban and Environmental Sciences, Laboratory for Earth Surface Processes, Sino-French Institute for Earth System Science, Peking University, Beijing 100871, China.

Atmospheric black carbon (BC) concentrations are governed by both emissions and meteorological conditions. Distinguishing these effects enables quantification of the effectiveness of emission mitigation actions by excluding meteorological effects. Here, we develop reduced-form models in both direct (RFDMs) and inverse (RFIMs) modes to estimate ambient BC concentrations. The models were developed based on outputs from multiyear simulations under three conditional scenarios with realistic or fixed emissions and meteorological conditions. We established a set of probabilistic functions (PFs) to quantify the meteorological influences. A significant two-way linear relationship between multiyear annual emissions and mean ambient BC concentrations was revealed at the grid cell scale. The correlation between them was more significant at grid cells with high emission densities. The concentrations and emissions at a given grid cell are also significantly correlated with emissions and concentrations of the surrounding areas, respectively, although to a lesser extent. These dependences are anisotropic depending on the prevailing winds and source regions. The meteorologically induced variation at the monthly scale was significantly higher than that at the annual scale. Of the major meteorological parameters, wind vectors, temperature, and relative humidity were found to most significantly affect variation in ambient BC concentrations.
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http://dx.doi.org/10.1021/acs.est.1c02174DOI Listing
July 2021

Unsupervised PM anomalies in China induced by the COVID-19 epidemic.

Sci Total Environ 2021 Jul 1;795:148807. Epub 2021 Jul 1.

Laboratory for Earth Surface Processes, College of Urban and Environmental Sciences, Peking University, Beijing 100871, China. Electronic address:

To stop the spread of COVID-19 (2019 novel coronavirus), China placed lockdown on social activities across China since mid-January 2020. The government actions significantly affected emissions of atmospheric pollutants and unintentionally created a nationwide emission reduction scenario. In order to assess the impacts of COVID-19 on fine particular matter (PM) levels, we developed a "conditional variational autoencoder" (CVAE) algorithm based on the deep learning to discern unsupervised PM anomalies in Chines cities during the COVID-19 epidemic. We show that the timeline of changes in number of cities with unsupervised PM anomalies is consistent with the timeline of WHO's response to COVID-19. Using unsupervised PM anomaly as a time node, we examine changes in PM before and after the time node to assess the response of PM to the COVID-19 lockdown. The rate of decrease of PM around the time node in northern China is 3.5 times faster than southern China, and decreasing PM levels in southern China is 3.5 times of that in northern China. Results were also compared with anomalous PM occurring in Chinese's Spring Festival from 2017 to 2019, PM anomalies during around Chinese New Year in 2020 differ significantly from 2017 to 2019. We demonstrate that this method could be used to detect the response of air quality to sudden changes in social activities.
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http://dx.doi.org/10.1016/j.scitotenv.2021.148807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8247192PMC
July 2021

Prediction of Hemorrhagic Transformation After Ischemic Stroke: Development and Validation Study of a Novel Multi-biomarker Model.

Front Aging Neurosci 2021 28;13:667934. Epub 2021 May 28.

Department of Neurology, Center of Cerebrovascular Diseases, West China Hospital, Sichuan University, Chengdu, China.

: We aimed to develop and validate a novel multi-biomarker model for predicting hemorrhagic transformation (HT) risk after acute ischemic stroke (AIS). : We prospectively included patients with AIS admitted within 24 h of stroke from January 1st 2016 to January 31st 2019. A panel of 17 circulating biomarkers was measured and analyzed in this cohort. We assessed the ability of individual circulating biomarkers and the combination of multiple biomarkers to predict any HT, symptomatic HT (sHT) and parenchymal hematoma (PH) after AIS. The strategy of multiple biomarkers in combination was then externally validated in an independent cohort of 288 Chinese patients. : A total of 1207 patients with AIS (727 males; mean age, 67.2 ± 13.9 years) were included as a derivation cohort, of whom 179 patients (14.8%) developed HT. The final multi-biomarker model included three biomarkers [platelets, neutrophil-to-lymphocyte ratios (NLR), and high-density lipoprotein (HDL)] from different pathways, showing a good performance for predicting HT in both the derivation cohort (c statistic = 0·64, 95% CI 0·60-0·69), and validation cohort (c statistic = 0·70, 95% CI 0·58-0·82). Adding these three biomarkers simultaneously to the basic model with conventional risk factors improved the ability of HT reclassification [net reclassification improvement (NRI) 65.6%, < 0.001], PH (NRI 64.7%, < 0.001), and sHT (NRI 71.3%, < 0.001). : This easily applied multi-biomarker model had a good performance for predicting HT in both the derivation and external validation cohorts. Incorporation of biomarkers into clinical decision making may help to identify patients at high risk of HT after AIS and warrants further consideration.
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http://dx.doi.org/10.3389/fnagi.2021.667934DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193036PMC
May 2021

A comparison investigation of atmospheric NMHCs at two sampling sites of Beijing city and a rural area during summertime.

Sci Total Environ 2021 Aug 10;783:146867. Epub 2021 Apr 10.

Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100085, China.

Atmospheric non-methane hydrocarbons (NMHCs) were measured synchronously at an urban site of Beijing city (BJ) and a rural site of Dongbaituo (DBT) in Hebei province from 1 July to 15 August 2016. The average concentration of the total NMHCs (TNMHCs) at DBT site were about a factor of 1.3 higher than that at BJ site. Ethane, ethylene, propane, acetylene, butane, isobutane, toluene and isopentane were the common species in the top ten NMHCs at the two sampling sites, and the contributions of the top ten NMHCs to TNMHCs at BJ and DBT sites were 65.6% and 75.1%, respectively. The diurnal variations of TNMHCs at BJ site exhibited one peak during the morning rush hours, whereas two peaks occurred at DBT site during the period from 3:00 to 8:00 (UTC/GMTC8). Based on the correlation coefficients of typical NMHCs pairs and the positive matrix factorization (PMF) results, the gasoline exhaust was found to be the dominant source (38.8%) for atmospheric NMHCs in Beijing, while coal combustion made the largest contribution (32.3%) at the rural site. Atmospheric ozone production over the BJ site was found to be NMHCs-sensitive, while it was in the transition regime at DBT site. Additionally, the largest contributions of atmospheric NMHCs groups to the ozone formation potential at BJ and DBT sites were alkenes and aromatics, with the proportions of 35.8% and 38.6%, respectively.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146867DOI Listing
August 2021

Ginsenoside Rb1 protects from Staphylococcus aureus-induced oxidative damage and apoptosis through endoplasmic reticulum-stress and death receptor-mediated pathways.

Ecotoxicol Environ Saf 2021 Aug 23;219:112353. Epub 2021 May 23.

College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China. Electronic address:

Acute lung injury (ALI) is acute uncontrolled inflammation of lung tissue that leads to high fatality both in human and animals. Staphylococcus aureus (S. aureus) could be an opportunistic, versatile bacterial etiology of ALI. Ginsenoside Rb1 (Rb1) is extracted from the Panax ginseng, which displays a wide range of biological and pharmacological effects. However, protective effects of Rb1 in S. aureus-induced ALI though endoplasmic reticulum (ER) stress and death receptor-mediated pathways have not yet been reported. Therefore, present study was planned with the aims to investigate the antioxidant and anti-apoptotic properties of Rb1 through regulation of ER stress as well as death receptor-mediated pathways in ALI induced by S. aureus in mice. In this study, four groups of healthy Kunming mice (n = 48) were used. The S. aureus (80 µl; 1 ×10 CFU/10 µl) was administered intranasally to establish mice model of ALI. After 24 h of onset of S. aureus-induced ALI, the mice were injected thrice with Rb1 (40 mg/kg) intraperitoneally six hours apart. Histopathology, enzyme linked immunosorbent assay (ELISA), real time quantitative polymerase chain reaction (RT-qPCR), Immunohistochemistry and western blotting assay were employed in the current study. Our results suggested that Rb1 administration save lungs from pulmonary injury by reducing wet to dry (W/D) ratio, protein levels, total cells, neutrophilic count, reactive oxygen species (ROS), myeloperoxidase (MPO), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (Gpx)1 depletion. Meanwhile, Rb1 therapy ameliorated histopathology alteration of lung tissue and pro-inflammatory cytokines secretion. The gene expression of ER stress marker (PERK, AFT-6, IRE1 and CHOP) were upregulated markedly (P < .05) in S. aureus-instilled groups, which was reduced by Rb1 administration that is reveled from the result findings of the RT-qPCR and immunoblot assay. The results of immunohistochemistry for CHOP indicated the increased expression in S. aureus groups which in turn ameliorated by Rb1 treatment. The mRNA expression demonstrated that death receptor-associated genes (FasL, Fas, FADD and caspase-8) showed up-regulation in S. aureus group. The similar findings were observed for the protein expression of caspase-8, FADD and Fas. Rb1 treatment markedly (P < .05) reversed protein and mRNA expression levels of these death receptor-associated genes when compared to the S. aureus group. Taken together, Rb1 attenuated S. aureus-induced oxidative damage via the ER stress-mediated pathway and apoptosis through death receptor-mediated pathway. Conclusively, our findings provide an insight into preventive mechanism of Rb1 in ALI caused by S. aureus and hence proven a scientific baseline for the therapeutic application of Rb1.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112353DOI Listing
August 2021

RAS-association domain family 1A regulates the abnormal cell proliferation in psoriasis via inhibition of Yes-associated protein.

J Cell Mol Med 2021 Jun 7;25(11):5070-5081. Epub 2021 May 7.

State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.

Psoriasis is a chronic, inflammatory skin disease with a high incidence and recurrence; however, its exact pathogenesis and aetiology remain unclear. This study aimed to analyse the effect of the upstream negative regulator RAS-association domain family 1A (RASSF1A) on Yes-associated protein (YAP) in psoriasis. Skin lesions of 22 patients with psoriasis and 19 healthy controls were used. Human epidermal keratinocytes stimulated by M5 (IL-1α, IL-17, IL-22, TNF-α and oncostatin M) were used to establish a psoriatic cell model. BALB/c mice treated with topical imiquimod were used to establish a psoriatic mouse model. As the methylation level of RASSF1A increased, its expression in psoriatic patients and mice model decreased. Addition of the methylation inhibitor 5-Aza-CdR or RASSF1A-overexpressing lentivirus vector increased RASSF1A and reduced YAP expression; meanwhile improved skin lesions, reduced cell proliferation, induced cell cycle arrest in the G0/G1 phase, increased apoptosis, reduced inflammatory cytokines and activities of ERK, STAT3 and NF-κB signalling pathways. The results indicated that RASSF1A could play a role in the treatment of psoriasis by inhibiting YAP expression. Based on these findings, targeted drugs that can inhibit the methylation or increase the expression of RASSF1A may be useful for treating psoriasis.
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http://dx.doi.org/10.1111/jcmm.16489DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178269PMC
June 2021

Archaeal extracellular vesicles are produced in an ESCRT-dependent manner and promote gene transfer and nutrient cycling in extreme environments.

ISME J 2021 Apr 26. Epub 2021 Apr 26.

Archaeal Virology Unit, Institut Pasteur, Paris, France.

Membrane-bound extracellular vesicles (EVs), secreted by cells from all three domains of life, transport various molecules and act as agents of intercellular communication in diverse environments. Here we demonstrate that EVs produced by a hyperthermophilic and acidophilic archaeon Sulfolobus islandicus carry not only a diverse proteome, enriched in membrane proteins, but also chromosomal and plasmid DNA, and can transfer this DNA to recipient cells. Furthermore, we show that EVs can support the heterotrophic growth of Sulfolobus in minimal medium, implicating EVs in carbon and nitrogen fluxes in extreme environments. Finally, our results indicate that, similar to eukaryotes, production of EVs in S. islandicus depends on the archaeal ESCRT machinery. We find that all components of the ESCRT apparatus are encapsidated into EVs. Using synchronized S. islandicus cultures, we show that EV production is linked to cell division and appears to be triggered by increased expression of ESCRT proteins during this cell cycle phase. Using a CRISPR-based knockdown system, we show that archaeal ESCRT-III and AAA+ ATPase Vps4 are required for EV production, whereas archaea-specific component CdvA appears to be dispensable. In particular, the active EV production appears to coincide with the expression patterns of ESCRT-III-1 and ESCRT-III-2, rather than ESCRT-III, suggesting a prime role of these proteins in EV budding. Collectively, our results suggest that ESCRT-mediated EV biogenesis has deep evolutionary roots, likely predating the divergence of eukaryotes and archaea, and that EVs play an important role in horizontal gene transfer and nutrient cycling in extreme environments.
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http://dx.doi.org/10.1038/s41396-021-00984-0DOI Listing
April 2021

The application analysis of 8F ultrafine chest drainage tube for thoracoscopic lobectomy of lung cancer.

J Cardiothorac Surg 2021 Apr 21;16(1):104. Epub 2021 Apr 21.

Department of Thoracic Surgery, Hebei Medical University Fourth Affiliated Hospital and Hebei Provincial Tumor Hospital, 12 Jiankang Road, Shijiazhuang, Hebei Province, 050011, People's Republic of China.

Background: Currently, thoracoscopic lobectomy is widely used in clinical practice, and postoperative placement of ultrafine drainage tube has advantages of reducing postoperative pain and accelerating postoperative recovery in patients. This study aimed to investigate the feasibility and safety of placement of 8F ultrafine chest drainage tube after thoracoscopic lobectomy and its superiority over traditional 24F chest drainage tube.

Methods: A retrospective data analysis was conducted in 169 patients who underwent placement of 8F ultrafine chest drainage tube or 24F chest drainage tube with thoracoscopic lobectomy for lung cancer from January 2018 to December 2019. Propensity score matching (PSM) was used to reduce bias between the experimental group and the control group. After PSM, 134 patients (67 per group) were enrolled. The drainage time, the total drainage volume, postoperative hospital stay, postoperative pain score and postoperative complication of both groups were analyzed and compared.

Results: Compared to group B, group A had lower pain scores on postoperative days 1, 2 and 3 (3.72 ± 0.65point vs 3.94 ± 0.67point, P = 0.027; 2.72 ± 0.93point vs 3.13 ± 1.04point, P = 0.016; and 1.87 ± 0.65point vs 2.39 ± 1.22point, P = 0.005), shorter drainage time (4.25 ± 1.79d vs 6.04 ± 1.96d, P = 0.000), fewer drainage volume (1100.42 ± 701.57 ml vs 1369.39 ± 624.25 ml, P = 0.021); and shorter postoperative hospital stay (8.46 ± 2.48d vs 9.37 ± 1.70d, P = 0.014). Postoperative complications such as subcutaneous emphysema, pulmonary infection, atelectasis, chest tube reinsertion and intrathoracic hemorrhage showed no differences between both groups (P > 0.05).

Conclusion: Compared with 24F chest drainage tube, the application of an 8F ultrafine chest drainage tube after thoracoscopic lobectomy has significantly shortened the drainage time, reduced the total drainage volume, reduced the postoperative pain degree, shortened the hospital day, and effectively detected postoperative intrathoracic hemorrhage. So, it is considered as an effective, safe and reliable drainage method.
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http://dx.doi.org/10.1186/s13019-021-01479-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059218PMC
April 2021

Six Metabolism Related mRNAs Predict the Prognosis of Patients With Hepatocellular Carcinoma.

Front Mol Biosci 2021 25;8:621232. Epub 2021 Feb 25.

Department of Hepatic Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

Hepatocellular carcinoma (HCC) is one of the most common aggressive solid malignant tumors and current research regards HCC as a type of metabolic disease. This study aims to establish a metabolism-related mRNA signature model for risk assessment and prognosis prediction in HCC patients. HCC data were obtained from The Cancer Genome Atlas (TCGA), International Cancer Genome Consortium (ICGC) and Gene Enrichment Analysis (GSEA) website. Least absolute shrinkage and selection operator (LASSO) was used to screen out the candidate mRNAs and calculate the risk coefficient to establish the prognosis model. A high-risk group and low-risk group were separated for further study depending on their median risk score. The reliability of the prediction was evaluated in the validation cohort and the whole cohort. A total of 548 differential mRNAs were identified from HCC samples ( = 374) and normal controls ( = 50), 45 of which were correlated with prognosis. A total of 373 samples met the screening criteria and there were randomly divided into the training cohort ( = 186) and the validation cohort ( = 187). In the training cohort, six metabolism-related mRNAs were used to construct a prognostic model with a LASSO regression model. Based on the risk model, the overall survival rate of the high-risk cohort was significantly lower than that of the low-risk cohort. The results of a time-ROC curve proved that the risk score (AUC = 0.849) had a higher prognostic value than the pathological grade, clinical stage, age or gender. The model constructed by the six metabolism-related mRNAs has a significant value for survival prediction and can be applied to guide the evaluation of HCC and the designation of clinical therapy.
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http://dx.doi.org/10.3389/fmolb.2021.621232DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045485PMC
February 2021

Simultaneous electricity generation and eutrophic water treatment utilizing iron coagulation cell with nitrification and denitrification biocathodes.

Sci Total Environ 2021 Aug 17;782:146436. Epub 2021 Mar 17.

School of Environment, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, No 73 Huanghe Road, Nangang District, Harbin 150090, China. Electronic address:

Anthropogenic nutrients released into water induce eutrophication and threaten aquatic life and human health. In this study, an Fe anode coagulation cell with nitrification and denitrification biocathodes was constructed for power generation and algae and nutrient removal. The nitrification and denitrification biocathodes achieved maximum power densities of 6.0 and 6.6 W/m, respectively. The algae (99.2 ± 0.5%), phosphate (97.4 ± 0.6%), and ammonia (23.1 ± 0.2%) were removed by a spontaneous electrocoagulation process in the anode chamber. In the nitrification biocathode chamber, 95.3 ± 1.4% of the ammonia was oxidized within 6 h, and 88.2 ± 2.5% of the nitrate was removed in 10 h in the denitrification biocathode chamber. The microbial community analysis revealed that ammonia removal was attributed to nitrifying bacteria, including Acinetobacter sp., Phycisphaera sp., and Nitrosomonas sp., and the dominant denitrifying bacteria in the denitrifying biocathode chamber were Planococcus sp., Exiguobacterium sp., and Lysinibacillus sp. In this study, the combination of Fe anodes and biocathodes is shown to afford an efficient method for the simultaneous algae and nutrient removal and power generation.
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http://dx.doi.org/10.1016/j.scitotenv.2021.146436DOI Listing
August 2021

Virus-induced cell gigantism and asymmetric cell division in archaea.

Proc Natl Acad Sci U S A 2021 Apr;118(15)

Archaeal Virology Unit, Institut Pasteur, 75015 Paris, France;

Archaeal viruses represent one of the most mysterious parts of the global virosphere, with many virus groups sharing no evolutionary relationship to viruses of bacteria or eukaryotes. How these viruses interact with their hosts remains largely unexplored. Here we show that nonlytic lemon-shaped virus STSV2 interferes with the cell cycle control of its host, hyperthermophilic and acidophilic archaeon , arresting the cell cycle in the S phase. STSV2 infection leads to transcriptional repression of the cell division machinery, which is homologous to the eukaryotic endosomal sorting complexes required for transport (ESCRT) system. The infected cells grow up to 20-fold larger in size, have 8,000-fold larger volume compared to noninfected cells, and accumulate massive amounts of viral and cellular DNA. Whereas noninfected cells divide symmetrically by binary fission, the STSV2-infected cells undergo asymmetric division, whereby giant cells release normal-sized cells by budding, resembling the division of budding yeast. Reinfection of the normal-sized cells produces a new generation of giant cells. If the CRISPR-Cas system is present, the giant cells acquire virus-derived spacers and terminate the virus spread, whereas in its absence, the cycle continues, suggesting that CRISPR-Cas is the primary defense system in against STSV2. Collectively, our results show how an archaeal virus manipulates the cell cycle, transforming the cell into a giant virion-producing factory.
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http://dx.doi.org/10.1073/pnas.2022578118DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8054024PMC
April 2021

Nidogen-1 expression is associated with overall survival and temozolomide sensitivity in low-grade glioma patients.

Aging (Albany NY) 2021 03 18;13(6):9085-9107. Epub 2021 Mar 18.

Department of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

We investigated the prognostic significance of nidogen-1 (NID1) in glioma. Oncomine, GEPIA, UALCAN, CCGA database analyses showed that NID1 transcript levels were significantly upregulated in multiple cancer types, including gliomas. Quantitative RT-PCR analyses confirmed that NID1 expression was significantly upregulated in glioma tissues compared to paired adjacent normal brain tissue samples (n=9). NID1 silencing enhanced apoptosis and the temozolomide sensitivity of U251 and U87-MG glioma cells. Protein-protein interaction network analysis using the STRING and GeneMANIA databases showed that NID1 interacts with several extracellular matrix proteins. TIMER database analysis showed that NID1 expression in low-grade gliomas was associated with tumor infiltration of B cells, CD4 and CD8 T cells, macrophages, neutrophils, and dendritic cells. Kaplan-Meier survival curve analysis showed that low-grade gliomas patients with high NID1 expression were associated with shorter overall survival. However, NID1 expression was not associated with overall survival in glioblastoma multiforme patients. These findings demonstrate that NID1 expression in glioma tissues is associated with overall survival of low-grade glioma patients and temozolomide sensitivity. NID1 is thus a potential prognostic biomarker and therapeutic target in low-grade glioma patients.
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http://dx.doi.org/10.18632/aging.202789DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8034893PMC
March 2021

Use of four genes in exosomes as biomarkers for the identification of lung adenocarcinoma and lung squamous cell carcinoma.

Oncol Lett 2021 Apr 3;21(4):249. Epub 2021 Feb 3.

Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China.

The determination of biomarkers in the blood specific for lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) is crucial for the selection of effective treatment strategies and the prediction of prognosis. The purpose of the present study was to analyze the differentially expressed genes (DEGs) in LUSC and LUAD from The Cancer Genome Atlas (TCGA) database. In order to identify the potential biomarkers for non-small cell lung cancer (NSCLC) for clinical diagnosis, bioinformatics was used to analyze the DEGs of two subtypes of NSCLC, LUAD and LUSC. Exosomes were isolated from the serum of patients with LUAD or LUSC and identified using transmission electron microscopy, nanoparticle tracking analysis and western blot analysis. A total of four differential exosomal mRNAs were selected for validation with serum samples from 70 patients with NSCLC via reverse transcription-quantitative polymerase chain reaction. Receiver operating characteristic curves were established to evaluate the clinical diagnostic value of four DEGs for patients with LUAD and LUSC. The analysis based on TCGA data revealed the DEGs in LUSC and LUAD: A total of 1,619 genes were differentially expressed in patients with LUSC and LUAD. DEGs analyzed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that inflammation-related signaling pathways, such as complement pathways, and multiple autoimmune diseases, such as systemic lupus erythematosus and asthma were mainly enriched in LUAD. The cell cycle, Hippo signaling pathway, Rap1 signaling pathway and Wnt signaling pathway were the main signaling pathways enriched in LUSC. The combination of tumor protein P63 (TP63), keratin 5 (KRT5), CEA cell adhesion molecule 6 (CEACAM6) and surfactant protein B (SFTPB) improved the specificity and sensitivity in the diagnosis of different lung cancer subtypes. Exosomal TP63, KRT5, CEACAM6 and SFTPB mRNAs can thus be used as biomarkers to differentiate between LUSC and LUAD, and may provide a novel strategy for their differential diagnosis and treatment.
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http://dx.doi.org/10.3892/ol.2021.12510DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7882885PMC
April 2021

Effect of the Annealing Atmosphere on Crystal Phase and Thermoelectric Properties of Copper Sulfide.

ACS Nano 2021 Mar 1;15(3):4967-4978. Epub 2021 Mar 1.

Catalonia Energy Research Institute - IREC, Sant Adrià de Besòs, 08930 Barcelona, Spain.

CuS has become one of the most promising thermoelectric materials for application in the middle-high temperature range. Its advantages include the abundance, low cost, and safety of its elements and a high performance at relatively elevated temperatures. However, stability issues limit its operation current and temperature, thus calling for the optimization of the material performance in the middle temperature range. Here, we present a synthetic protocol for large scale production of covellite CuS nanoparticles at ambient temperature and atmosphere, and using water as a solvent. The crystal phase and stoichiometry of the particles are afterward tuned through an annealing process at a moderate temperature under inert or reducing atmosphere. While annealing under argon results in CuS nanopowder with a rhombohedral crystal phase, annealing in an atmosphere containing hydrogen leads to tetragonal CuS. High temperature X-ray diffraction analysis shows the material annealed in argon to transform to the cubic phase at . 400 K, while the material annealed in the presence of hydrogen undergoes two phase transitions, first to hexagonal and then to the cubic structure. The annealing atmosphere, temperature, and time allow adjustment of the density of copper vacancies and thus tuning of the charge carrier concentration and material transport properties. In this direction, the material annealed under Ar is characterized by higher electrical conductivities but lower Seebeck coefficients than the material annealed in the presence of hydrogen. By optimizing the charge carrier concentration through the annealing time, CuS with record figures of merit in the middle temperature range, up to 1.41 at 710 K, is obtained. We finally demonstrate that this strategy, based on a low-cost and scalable solution synthesis process, is also suitable for the production of high performance CuS layers using high throughput and cost-effective printing technologies.
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http://dx.doi.org/10.1021/acsnano.0c09866DOI Listing
March 2021

IFN-τ Attenuates LPS-Induced Endometritis by Restraining HMGB1/NF-κB Activation in bEECs.

Inflammation 2021 Aug 18;44(4):1478-1489. Epub 2021 Feb 18.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.

Endometritis is a common inflammatory disease in uterine tissues that leads to animal infertility. Among the causes, Escherichia coli infection is one of the main reasons. Interferon-tau (IFN-τ) is the initial pregnancy signal for ruminant embryos and can induce immune tolerance in humans and other species. However, there are scarce reports on whether IFN-τ has a regulatory effect on endometrial inflammatory damage through HMGB1-NF-κB signalling. The purpose of this study was to investigate the regulatory mechanism of IFN-τ in HMGB1-NF-κB signalling in LPS-induced endometritis. ELISA and qPCR were used to detect the expression of LPS-induced pro-inflammatory cytokines in bovine endometrial epithelial cells (bEECs or BEND) under IFN-τ intervention, and the levels of HMGB1, p-IKK and p-p65 were detected by Western blotting. The nuclear translocation of NF-κB p65 was determined through immunofluorescence. In addition, bEECs were transfected with si-HMGB1 to elucidate the key role of HMGB1 and IFN-τ in the endometrial inflammatory cascade. The results indicated that IFN-τ inhibits the expression of related pro-inflammatory cytokines in an inflammatory injury model of bovine endometrial epithelial cells induced by LPS. Furthermore, experiments have proven that IFN-τ has protective effects on E. coli endotoxin-induced endometritis in mice in vivo. IFN-τ inhibited the HMGB1-NF-κB axis and significantly reduced the secretion of pro-inflammatory cytokines, the expression of HMGB1 protein and the levels of IKK and NF-κB p65 phosphorylation. In summary, our results showed that IFN-τ resists E. coli endotoxin-induced endometritis by attenuating HMGB1/NF-κB signalling.
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http://dx.doi.org/10.1007/s10753-021-01433-yDOI Listing
August 2021

Multi-shelled hollow layered double hydroxides with enhanced performance for the oxygen evolution reaction.

Chem Commun (Camb) 2021 Mar 17;57(22):2752-2755. Epub 2021 Feb 17.

Institute for New Energy Materials & Low-Carbon Technologies and Tianjin Key Lab for Photoelectric Materials & Devices, School of Materials Science and Engineering, Tianjin University of Technology, Tianjin 300384, China.

Hollow materials with a sophisticated structure are promising for various applications with boosted performances and innovative properties. Herein, we report an in situ transformation strategy using multi-layered MOFs as templates to fabricate multi-shelled hollow NiZnCoFe layered double hydroxides (LDHs), which outperformed the double- and single-shelled hollow LDHs and commercial IrO in the oxygen evolution reaction.
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http://dx.doi.org/10.1039/d0cc07643kDOI Listing
March 2021

The effectiveness and safety of lienal polypeptide combined with chemotherapy or chemoradiotherapy for non-small cell lung cancer patients in real world.

Medicine (Baltimore) 2021 Jan;100(4):e24272

Department of Thoracic Surgery, Fourth Hospital, Hebei Medical University, Shijiazhuang, China.

Abstract: Chemotherapy/chemoradiotherapy are still the fundamental treatment for advanced lung cancers. To reduce side effects and improve life quality, lienal polypeptide (LP) could be used in combine with chemotherapy/chemoradiotherapy. Moreover, LP could regulate immune system and possibly reduce the side effects of chemotherapy drugs.In our study, 1658 lung cancer patients from 10 hospitals were retrospectively analyzed and divided into LP group and non-LP group by whether using LP during their treatment. Kaplan-Meier curves and Log-rank test was used to detect the difference of progression-free survival and overall-survival between the 2 groups. Two-sided P-values of less than .05 indicated statistical significance. All analyses were performed with SAS software (version 9.4 SAS Institute, Cary, NC).Results showed that the number of patients who had progressed diseases in LP group and control group were 532 (64.2%) and 507 (61.2%). Log Rank test showed that median progression-free survival for LP group was 12.1 months and 11.4 months for control group (P = .3478). Statistical analyses revealed significantly difference in overall-survival between LP group and control group (23.6 months vs 18.9 months, P = .0177). The overall adverse effect rates were non-significantly different with 9.9% in the LP group and 9.3% in the non-LP group (P = .6767).In conclusion, our research results indicated that LP used in combination with chemotherapy/chemoradiotherapy was a safe and effective treatment for patients of advanced lung cancer. LP could also reduce the adverse effects of chemotherapy/chemoradiotherapy, thereby improving patients' life qualities, and potentially improving prognosis.
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http://dx.doi.org/10.1097/MD.0000000000024272DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7850644PMC
January 2021

Applications of microcapillary films in bioanalytical techniques.

Analyst 2021 Mar 2;146(5):1529-1537. Epub 2021 Feb 2.

Institute of Process Equipment, College of Energy Engineering, Zhejiang University, Hangzhou 310027, China.

Microcapillary film (MCF) is an extruded plastic film with an array of parallel microcapillaries (30-500 μm) and it has wide potential applications in bioanalytical techniques as a microfluidic platform. With different surface modification strategies, an MCF combines the advantages of its structure and modified chemical properties to realize various bioanalytical functions. In this review, we begin by introducing the manufacturing process of MCFs, common materials used to produce MCFs, surface treatment approaches of inner surfaces, and a signal detection and readout system of the MCF platform. Then, we summarize some typical applications of MCFs, particularly in protein chromatography, Escherichia coli detection for urinary tract infections, prostate-specific antigen detection for prostate cancer and multiplex immunoassays. Finally, future perspectives of MCFs in bioanalytical techniques are discussed.
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http://dx.doi.org/10.1039/d0an01945cDOI Listing
March 2021

Structure-guided analysis of Arabidopsis JASMONATE-INDUCED OXYGENASE (JOX) 2 reveals key residues for recognition of jasmonic acid substrate by plant JOXs.

Mol Plant 2021 May 28;14(5):820-828. Epub 2021 Jan 28.

State Key Laboratory of Agrobiotechnology, Ministry of Agriculture Key Laboratory for Crop Pest Monitoring and Green Control, Joint International Research Laboratory of Crop Molecular Breeding, College of Plant Protection, China Agricultural University, Beijing 100193, China. Electronic address:

The jasmonic acid (JA) signaling pathway is used by plants to control wound responses. The persistent accumulation of JA inhibits plant growth, and the hydroxylation of JA to 12-hydroxy-JA by JASMONATE-INDUCED OXYGENASEs (JOXs, also named jasmonic acid oxidases) is therefore vital for plant growth, while structural details of JA recognition by JOXs are unknown. Here, we present the 2.65 Å resolution X-ray crystal structure of Arabidopsis JOX2 in complex with its substrate JA and its co-substrates 2-oxoglutarate and Fe(II). JOX2 contains a distorted double-stranded β helix (DSBH) core flanked by α helices and loops. JA is bound in the narrow substrate pocket by hydrogen bonds with the arginine triad R225, R350, and R354 and by hydrophobic interactions mainly with the phenylalanine triad F157, F317, and F346. The most critical residues for JA binding are F157 and R225, both from the DSBH core, which interact with the cyclopentane ring of JA. The spatial distribution of critical residues for JA binding and the shape of the substrate-binding pocket together define the substrate selectivity of the JOXs. Sequence alignment shows that these critical residues are conserved among JOXs from higher plants. Collectively, our study provides insights into the mechanism by which higher plants hydroxylate the hormone JA.
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http://dx.doi.org/10.1016/j.molp.2021.01.017DOI Listing
May 2021

Evaluation of modified permeable pavement systems with coal gangue to remove typical runoff pollutants under simulated rainfall.

Water Sci Technol 2021 Jan;83(2):381-395

Key Laboratory of Urban Stormwater System and Water Environment, Ministry of Education, Beijing University of Civil Engineering and Architecture, Beijing 102616, China E-mail: Beijing Engineering Research Center of Sustainable Urban Sewage System Construction and Risk Control, Beijing University of Civil Engineering and Architecture, Beijing 100044, China.

Coal gangue (CG) as mineral waste was properly treated and applied as the filter media in permeable pavement systems due to its good sorption ability and mechanical strength. Batch experiments show the maximum adsorption capacity of calcined CG to phosphorus could reach 2.63 mg/g. To evaluate the removal effect of typical runoff pollutants including chemical oxygen demand (COD), total nitrogen (TN), total phosphorus (TP) and ammonia (NH-N), the gravel and sand in a traditional pavement system were replaced by CG respectively. The leaching behavior of the four pollutants in CG modified systems is limited and comparable with traditional system, indicating pretreated CG as filler would not cause environmental risk. CG-based pavement systems improved the removal efficiency of the four pollutants especially for TP. The removal mechanisms including interception, adsorption and microorganism degradation. The removal rates of COD, NH-N and TN by CG modified and traditional systems decreased with rainfall duration, while it is not obviously changed with rainfall recurrence period and drying period. Overall, the permeable pavement with CG layers that replaced both sand and gravel layers show best removal efficiency of all pollutants investigated especially for TP (>95%). This study provides a new way for CG utilization and gives the reference for the process design of permeable pavement.
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http://dx.doi.org/10.2166/wst.2020.574DOI Listing
January 2021

The effect of continuous proximal adductor block combined with IPACK block on ERAS in total knee arthroplasty.

Minerva Med 2021 Jan 19. Epub 2021 Jan 19.

Department of Anesthesiology, Dongying Second People's Hospital, Dongying, Shandong, China -

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http://dx.doi.org/10.23736/S0026-4806.20.07247-XDOI Listing
January 2021

Association between admission blood fibrinogen-to-albumin ratio and clinical outcomes after acute lacunar stroke.

Biomark Med 2021 02 14;15(2):87-96. Epub 2021 Jan 14.

Department of Neurology, Center of Cerebrovascular Diseases, West China Hospital of Sichuan University, Chengdu, 610041, China.

We aimed to investigate the influence of admission fibrinogen-to-albumin ratio (FAR) on 3-month outcomes after acute lacunar stroke. Consecutive patients with acute lacunar stroke were included and classified into two groups according to an optimized FAR cut-off value determined by receiver operating characteristic curve analysis. Compared with those with low FAR (<0.077), patients from the high FAR group (≥0.077) had significantly higher risk for 3-month disability and the composite outcome of death/disability. After logistic regression adjustment, high FAR was still significantly associated with 3-month disability and death/disability. FAR ≥0.077 on admission might be an independent predictor of disability and death/disability at 3 months after lacunar stroke, which needs to be verified in future studies.
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http://dx.doi.org/10.2217/bmm-2019-0537DOI Listing
February 2021

Effect of Travel Restrictions of Wuhan City Against COVID-19: A Modified SEIR Model Analysis.

Disaster Med Public Health Prep 2021 Jan 8:1-7. Epub 2021 Jan 8.

Institute of Disaster Medicine, Tianjin University, Tianjin, P.R. China.

Objective: Since December 2019, a new coronavirus viral was initially detected in Wuhan, China. Population migration increases the risk of epidemic transmission. Here, the objective of study is to estimate the output risk quantitatively and evaluate the effectiveness of travel restrictions of Wuhan city.

Methods: We proposed a modified susceptible-exposed-infectious-recovered (SEIR) dynamics model to predict the number of coronavirus disease 2019 (COVID-19) symptomatic and asymptomatic infections in Wuhan. And, subsequently, we estimated the export risk of COVID-19 epidemic from Wuhan to other provinces in China. Finally, we estimated the effectiveness of travel restrictions of Wuhan city quantitatively by the export risk on the assumption that the measure was postponed.

Results: The export risks of COVID-19 varied from Wuhan to other provinces of China. The peak of export risk was January 21-23, 2020. With the travel restrictions of Wuhan delayed by 3, 5, and 7 d, the export risk indexes will increase by 38.50%, 55.89%, and 65.63%, respectively.

Conclusions: The results indicate that the travel restrictions of Wuhan reduced the export risk and delayed the overall epidemic progression of the COVID-19 epidemic in China. The travel restrictions of Wuhan city may provide a reference for the control of the COVID-19 epidemic all over the world.
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http://dx.doi.org/10.1017/dmp.2021.5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027550PMC
January 2021

Mechanism of CuSO cytotoxicity in goat erythrocytes after high-level in vitro exposure to isotonic media.

Ecotoxicol Environ Saf 2021 Jan 9;208:111730. Epub 2020 Dec 9.

College of Animal Science, Tarim University, Alar, Xinjiang Uygur Autonomous Region 843300, PR China; Key Laboratory of Tarim Animal Husbandry Technology Corps, Tarim University, Alar, Xinjiang Uygur Autonomous Region 843300, PR China. Electronic address:

Copper (Cu) is a common environmental pollutant in nature. Cu-poisoning can cause liver damage and erythrocytes hemolysis. To evaluate the effect of CuSO poisoning on the morphological and functional characteristics of goat red blood cells. Five 10-14-month-old goats were selected for jugular vein blood sampling to obtain erythrocytes, and then the erythrocytes were processed with different concentrations (0, 10, 20, 30, 40 and 50 μmol/L) of CuSO for 48 h, and 40 μmol/L doses CuSO incubated for different time (12, 24, 36, 48 and 60 h) to process erythrocytes. We observed the changes in erythrocyte morphology through scanning electron microscopy, and detected the antioxidant function and activities of three ATPases. Additionally, biological properties were examined from the perspectives of phospholipids and membrane protein components, permeability fragility, and fluidity in erythrocytes. We found that after CuSO treatment, the antioxidant capacity of erythrocytes decreased, which was manifested as increased MDA content and decreased CuZn-SOD and GSH-Px activities (p < 0.05). In addition, we also found that erythrocyte fluidity decreased, osmotic fragility increased, membrane phospholipid percentage and protein composition changes abnormally, and Na/K-ATPase Mg-ATPase and Ca-ATPase activities decreased (p < 0.05). From the results, it can be concluded that CuSO exposure causes hemolysis of goat erythrocytes through oxidative stress to the structure and function of erythrocytes, showing a dose-time effect.
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http://dx.doi.org/10.1016/j.ecoenv.2020.111730DOI Listing
January 2021

Endometrial extracellular matrix rigidity and IFNτ ensure the establishment of early pregnancy through activation of YAP.

Cell Prolif 2021 Feb 4;54(2):e12976. Epub 2021 Jan 4.

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.

Background: In mammals, early pregnancy is a critical vulnerable period during which complications may arise, including pregnancy failure. Establishment of a maternal endometrial acceptance phenotype is a prerequisite for semiheterogeneous embryo implantation, comprising the rate-limiting step of early pregnancy.

Methods: Confocal fluorescence, immunohistochemistry and western blot for nuclear and cytoplasmic protein were used to examine the activation of yes-associated protein (YAP) in uterine tissue and primary endometrial cells. The target binding between miR16a and YAP was verified by dual-luciferase reporter gene assay. The mouse pregnancy model and pseudopregnancy model were used to investigate the role of YAP in the maternal uterus during early pregnancy in vivo.

Results: We showed that YAP translocates into the nucleus in the endometrium of cattle and mice during early pregnancy. Mechanistically, YAP acts as a mediator of ECM rigidity and cell density, which requires the actomyosin cytoskeleton and is partially dependent on the Hippo pathway. Furthermore, we found that the soluble factor IFNτ, which is a ruminant pregnancy recognition factor, also induced activation of YAP by reducing the expression of miR-16a.

Conclusions: This study revealed that activation of YAP is necessary for early pregnancy in bovines because it induced cell proliferation and established an immunosuppressive local environment that allowed conceptus implantation into the uterine epithelium.
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http://dx.doi.org/10.1111/cpr.12976DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849163PMC
February 2021

Utilization of circulating cell-free DNA profiling to guide first-line chemotherapy in advanced lung squamous cell carcinoma.

Theranostics 2021 1;11(1):257-267. Epub 2021 Jan 1.

Department of Oncology, Qingdao Municipal Hospital, Qingdao, Shandong, China.

Platinum-based chemotherapy is one of treatment mainstay for patients with advanced lung squamous cell carcinoma (LUSC) but it is still a "one-size fits all" approach. Here, we aimed to investigate the predictive and monitoring role of circulating cell-free DNA (cfDNA) profiling for the outcome of first-line chemotherapy in patients with advanced LUSC. Peripheral blood samples of 155 patients from a phase IV trial and 42 cases from an external real-world cohort were prospectively collected. We generated a copy number variations-based classifier via machine learning algorithm to integrate molecular profiling of cfDNA, named RESPONSE SCORE (RS) to predict the treatment outcome. To monitor the treatment efficacy, cfDNA samples collected at different time points were subjected to an ultra-deep sequencing platform. The results showed that patients with high RS showed substantially higher objective response rate than those with low RS in training set ( < 0.001), validation set ( < 0.001) and real-world cohort ( = 0.019). Furthermore, a significant difference was observed in both progression-free survival (training set, < 0.001; validation set: < 0.001; real-world cohort: = 0.019) and overall survival (training set, < 0.001; validation set: = 0.037) between high and low RS group. Notably, variant allele frequency (VAF) calculated from an ultra-deep sequencing platform significantly reduced in patients experienced a complete or partial response after 2 cycles of chemotherapy ( < 0.001), while it significantly increased in these of non-responder ( < 0.001). Moreover, VAF undetectable after 2 cycles of chemotherapy was correlated with markedly better objective response rate ( < 0.001) and progression-free survival ( < 0.001) than those with detectable VAF. These findings indicated that the RS, a circulating cfDNA sequencing-based stratification index, could help to guide first-line chemotherapy in advanced LUSC. The change of VAF is valuable to monitor the treatment response.
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http://dx.doi.org/10.7150/thno.51243DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7681090PMC
January 2021

The role of prognostic nutritional index in the management of pulmonary sarcomatoid carcinoma.

Clin Sarcoma Res 2020 Dec 7;10(1):26. Epub 2020 Dec 7.

Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, No. 12, Jiankang Road, Shijiazhuang, 050000, China.

Background: Pulmonary sarcomatoid carcinoma is characterized by poor survival rates compared with other non-small cell lung cancer. Prognostic nutritional index has significant prognostic value in many malignant tumors. We conducted this retrospective study to investigate the role of prognostic nutritional index in patients with pulmonary sarcomatoid carcinoma and to determine prognostic factors.

Methods: Of 8176 patients with resected lung cancer in a single high-volume institution between 2008 and 2015, 91 patients with pathologically diagnosed sarcomatoid carcinoma were included in our study and evaluated. Kaplan-Meier analysis and Cox regression analysis were conducted to analyze clinicopathologic data. Subgroup analysis of overall survival (OS) and recurrence-free survival (RFS) among pulmonary sarcomatoid carcinoma patients were also conducted.

Results: Univariable analysis showed that tumor size (P = 0.018 in OS), and P = 0.021 in RFS), tumor stage(P < 0.001 in OS, and P = 0.002 in RFS), nodal metastasis (P < 0.001 in OS, and P < 0.001 in RFS), pathological stage (P < 0.001 in OS, and P < 0.001 in RFS), treatment modality (P = 0.032 in OS, and P = 0.059 in RFS) and PNI (P < 0.001 in OS, and P < 0.001 in RFS), were significant factors of both OS and RFS. In multivariable analysis, for OS, the pathological stage (Hazard ratio (HR) 1.432; 95% confidence interval (95% CI) 1.210-1.695; P < 0.001) and PNI (HR 0.812; 95% CI 0.761-0.865; P < 0.001) were independent prognostic factors. And for RFS, We found PNI as an independent prognostic factor (HR 0.792; 95% CI 0.739-0.848; P < 0.001), and the pathological stage (HR 1.373; 95% CI 1.160-1.625; P < 0.001). In the subgroup of patients with PNI ≥ 49.4, univariable analysis showed treatment modality was a significant factor of overall survival (P = 0.001); multivariable analysis showed patients received postoperative chemotherapy (HR 0.288; 95% CI 0.095-0.874; P = 0.028) or postoperative chemotherapy with targeted therapy (HR 0.148; 95% CI 0.030-0.726; P = 0.019) has better overall survival rates.

Conclusion: The PNI and the pathological TNM stage are independent prognostic factors for pulmonary sarcomatoid carcinoma. PNI is an important indicator for the selection of postoperative adjuvant therapy. Patients with PNI ≥ 49.4 may benefit from postoperative chemotherapy and targeted therapy. We still need further prospective studies to confirm these results.
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http://dx.doi.org/10.1186/s13569-020-00148-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7722437PMC
December 2020

Mutational Characterization and Potential Prognostic Biomarkers of Chinese Patients with Esophageal Squamous Cell Carcinoma.

Onco Targets Ther 2020 14;13:12797-12809. Epub 2020 Dec 14.

Department of Thoracic Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, People's Republic of China.

Purpose: Esophageal squamous cell carcinoma (ESCC) is the most common type of esophageal cancer in China and the 5-year mortality rate is up to 70%. Studies on the ESCC genetic landscape are needed to further explore clinical therapeutic strategies. In this study, we evaluated the genetic landscape of ESCC to aid the search for clinical therapeutic strategies.

Patients And Methods: A total of 225 ESCC patients were enrolled in this study. Deep sequencing of 450 cancer genes was performed on formalin-fixed paraffin-embedded tumor biopsies and matched blood samples from patients. Tumor mutational burden (TMB) was calculated using an algorithm developed in-house.

Results: Our results showed that the most commonly mutated genes in ESCC were (96%), (46%), (44%), (44%), (44%), (31%), (26%), (24%), (18%), (16%), and (16%). We found that TMB correlated with patient drinking status. We identified mutations associated with sex, early ESCC, high TMB, and metastasis lymph nodes. mutations associated with sex ( = 0.035), tumor stage ( = 0.016), high TMB ( = 0.0072), and overall survival of patients ( = 0.0026). mutations associated with high TMB ( = 0.0016) and metastasis-positive lymph nodes ( = 0.027). These results suggested that and could be potential prognosis biomarkers for Chinese patients with ESCC. We also found that the number of positive lymph nodes was associated with disease-free survival. Clinical target gene analysis indicated that nearly half of Chinese ESCC patients might benefit from treatment with gene-specific target drugs.

Conclusion: Our study revealed the ESCC mutational landscape in 225 Chinese patients and uncovered the potential prognosis biomarker for Chinese patients with ESCC.
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http://dx.doi.org/10.2147/OTT.S275688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7751839PMC
December 2020

Role of YAP-related T cell imbalance and epidermal keratinocyte dysfunction in the pathogenesis of atopic dermatitis.

J Dermatol Sci 2021 Mar 18;101(3):164-173. Epub 2020 Dec 18.

State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, China; Department of Dermatology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, China; Guangdong Provincial Key Laboratory of Chinese Medicine for Prevention and Treatment of Refractory Chronic Disease, China. Electronic address:

Background: Atopic dermatitis (AD) is characterized by impaired skin barrier function and immune system dysfunction. The expression and role of Yes-associated protein (YAP) in AD are unclear.

Objective: To characterize the role of the YAP in T cell imbalance and epidermal keratinocyte dysfunction in the pathogenesis of AD.

Methods: We included 35 patients with AD (21 acute and 14 chronic). An AD mouse model was constructed using 2,4-dinitrofluorobenzene, and AD-like inflammatory cell model was constructed using TNF-α/IFN-γ-activated HaCaT cells. The proportion of Th1/Th2/Th17/Treg cells was detected using flow cytometry. After mononuclear cells were obtained from human peripheral blood or mouse spleen and induced to differentiate into different T cell subsets, YAP mRNA and protein expression were analyzed. Up-regulation of YAP was induced by lentivirus and down-regulation of YAP was induced by its specific inhibitor verteporfin (VP). The expression of YAP in skin lesions and infiltrating T cell subsets was detected using immunohistochemistry and double immunofluorescence staining, respectively.

Results: We found differing degrees of Th1/Th2/Th17/Treg imbalance in acute and chronic AD. YAP expression was downregulated in Treg cells and upregulated in Th17 cells; YAP expression was downregulated in the AD epidermis. After YAP overexpression, the proportion of both Th17 and the Treg cells differentiated from mouse spleen mononuclear cells increased. There was an opposite trend after YAP inhibition. The proliferation and migration decreased and apoptosis increased after YAP inhibition in HaCaT cells.

Conclusion: Change of YAP expression may cause T cell imbalance and hamper the healing of the epidermis in AD.
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http://dx.doi.org/10.1016/j.jdermsci.2020.12.004DOI Listing
March 2021

Novel one-dimensional [email protected] nanofibers: direct solid-state synthesis and applications in electrocatalytic water splitting.

Chem Commun (Camb) 2021 Jan;57(6):769-772

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.

Encapsulating metal nanoparticles with a graphitic carbon shell to remit the loss of active sites has drawn attention in catalysis. Herein, we report the development of a facile strategy to prepare graphitic carbon encapsulated Cu nanoparticle ([email protected]) nanofibers by in situ pyrolysis of organic-layered copper hydroxides, which exhibited superior activity and durability for water splitting.
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http://dx.doi.org/10.1039/d0cc06992bDOI Listing
January 2021
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