Publications by authors named "Junfeng Li"

237 Publications

Mild magnetic nanoparticle hyperthermia promotes the disaggregation and microglia-mediated clearance of Beta-amyloid plaques.

Nanomedicine 2021 Apr 13:102397. Epub 2021 Apr 13.

School of Biomedical Sciences, Kent State University, Kent, OH, 44242, USA; Department of Biological Sciences, Kent State University, Kent, OH 44242, USA. Electronic address:

The formation of beta-amyloid (Aβ) plaques is a classical hallmark of Alzheimer's disease (AD) that is associated with the promotion of neuroinflammation and subsequent neurotoxicity. Given the limited therapeutic options for targeting and clearing Aβ plaques in AD, there is an urgent need to develop effective approaches to reduce plaque accumulation. The objective of this study was to validate mild magnetic nanoparticle (MNP) hyperthermia technology as a strategy to clear Aβ deposits and determine the impact on microglia functionality. Our results demonstrated that the heating of MNPs localized to Aβ aggregates upon exposure to high frequency alternating magnetic field (AMF) was sufficient to disrupt Aβ plaques, resulting in its fragmentation. Importantly, this could facilitate the phagocytic clearance of Aβ as well as attenuate pro-inflammatory responses by human microglial cells. Our results support the feasibility of mild MNP/AMF hyperthermia as a new strategy for reducing beta-amyloid burdens in Alzheimer's disease.
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http://dx.doi.org/10.1016/j.nano.2021.102397DOI Listing
April 2021

Efficient Synthesis of α-FeOOH from Pickling Wastewater in Falling Film Tower and Its Kinetics.

ACS Omega 2021 Mar 16;6(12):8394-8402. Epub 2021 Mar 16.

School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, Hubei, China.

An efficient way to synthesize α-FeOOH from pickling wastewater in a falling film tower was proposed for the first time. This method overcomes the shortcomings of the traditional air oxidation method, and its production efficiency is increased by 16 times. The purity of α-FeOOH synthesized from pickling wastewater can reach 96.3%, and the iron recovery rate is greater than 90%. At the same time, we have systematically studied its kinetics in the falling film tower. The reaction rate constant at different temperatures was also determined with the activation energy = 32.2497 kJ/mol and the pre-exponential = 47.4132 s. In addition, based on the double-film theory, a corresponding macrokinetic model was established. Also, the Hatta number in the reaction system was obtained, which proved the excellent gas-liquid mass transfer performance in the falling film tower. This work provides a promising method for the efficient production of α-FeOOH and the recycling of pickling wastewater.
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http://dx.doi.org/10.1021/acsomega.1c00125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8015130PMC
March 2021

Ramatroban-Based Analogues Containing Fluorine Group as Potential F-Labeled Positron Emission Tomography (PET) G-Protein Coupled Receptor 44 (GPR44) Tracers.

Molecules 2021 Mar 6;26(5). Epub 2021 Mar 6.

Department of Translational Research & Cellular Therapeutics, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA.

Diabetes remains one of the fastest growing chronic diseases and is a leading source of morbidity and accelerated mortality in the world. Loss of beta cell mass (BCM) and decreased sensitivity to insulin underlie diabetes pathogenesis. Yet, the ability to safely and directly assess BCM in individuals with diabetes does not exist. Measures such as blood glucose provide only a crude indirect picture of beta cell health. PET imaging could, in theory, allow for safe, direct, and precise characterization of BCM. However, identification of beta cell-specific radiolabeled tracers remains elusive. G-protein coupled receptor 44 (GPR44) is a transmembrane protein that was characterized in 2012 as highly beta cell-specific within the insulin-positive islets of Langerhans. Accordingly, radiolabeling of existing GPR44 antagonists could be a viable method to accelerate PET tracer development. The present study aims to evaluate and summarize published analogues of the GPR44 antagonist ramatroban to develop F-labeled PET tracers for BCM analysis. The 77 corresponding ramatroban analogues containing a fluorine nuclide were characterized for properties including binding affinity, selectivity, and pharmacokinetic and metabolic profile, and 32 compounds with favorable properties were identified. This review illustrates the potential of GPR44 analogues for the development of PET tracers.
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http://dx.doi.org/10.3390/molecules26051433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961607PMC
March 2021

Characterizations of heavy metal contamination, microbial community, and resistance genes in a tailing of the largest copper mine in China.

Environ Pollut 2021 Mar 17;280:116947. Epub 2021 Mar 17.

Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China. Electronic address:

Copper mine tailings are causing great environmental concern nowadays due to their high contents of heavy metals. These hazards may release to air, water, and soil, posing great threat to the living organisms in the surroundings. In the present work, we profiled the heavy metal contents, microbiome and resistome of a mine tailing in Dexing Copper Mine, which is the largest open-pit copper mine in China. A total of 39.75 Gb clean data was generated by metagenomics sequencing and taxonomy analysis revealed Actinobacteria, Proteobacteria, Acidobacteria, Euryarchaeota, and Nitrospirae as the most abundant phylum in this tailing. In general, 76 heavy metal resistance genes (HMRGs) and 194 antimicrobial resistance genes (ARGs) were identified with merA and rpoB2 as the most abundant HMRG and ARG, respectively. We also compared the differences of heavy metal concentrations among the six sampling sites in the same tailing and found that significant differences exited in copper and zinc. Hierarchical cluster analysis showed that the samples from the six sampling sites were clustering in two groups based on heavy metal concentrations. Accordingly, clustering based on microbial composition and relative abundances of resistance genes exhibited the same clustering pattern, indicating a possible shaping influence of heavy metals on the microbiome and resistome in this tailing. Our work presented heavy metal contents, microbial composition and resistance genes in a copper mine tailing of the largest copper mine in China, and these data will of great use in the surveillance, maintenance, and remediation of this tailing.
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http://dx.doi.org/10.1016/j.envpol.2021.116947DOI Listing
March 2021

Association between urinary per- and poly-fluoroalkyl substances and COVID-19 susceptibility.

Environ Int 2021 Mar 19;153:106524. Epub 2021 Mar 19.

School of Medicine, University of California, San Diego, CA, USA. Electronic address:

Background And Objective: The growing impact of the COVID-19 pandemic has heightened the urgency of identifying individuals most at risk of infection. Per- and poly-fluoroalkyl substances (PFASs) are manufactured fluorinated chemicals widely used in many industrial and household products. The objective of this case-control study was to assess the association between PFASs exposure and COVID-19 susceptibility and to elucidate the metabolic dysregulation associated with PFASs exposure in COVID-19 patients.

Methods: Total 160 subjects (80 COVID-19 patients and 80 symptom-free controls) were recruited from Shanxi and Shandong provinces, two regions heavily polluted by PFASs in China. Twelve common PFASs were quantified in both urine and serum. Urine metabolome profiling was performed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).

Results: In unadjusted models, the risk of COVID-19 infection was positively associated with urinary levels of perfluorooctanesulfonic acid (PFOS) (Odds ratio: 2.29 [95% CI: 1.52-3.22]), perfluorooctanoic acid (PFOA) (2.91, [1.95-4.83], and total PFASs (∑ (12) PFASs) (3.31, [2.05-4.65]). After controlling for age, sex, body mass index (BMI), comorbidities, and urine albumin-to-creatinine ratio (UACR), the associations remained statistically significant (Adjusted odds ratio of 1.94 [95% CI: 1.39-2.96] for PFOS, 2.73 [1.71-4.55] for PFOA, and 2.82 [1.97-3.51] for ∑ (12) PFASs). Urine metabolome-PFASs association analysis revealed that 59% of PFASs-associated urinary endogenous metabolites in COVID-19 patients were identified to be produced or largely regulated by mitochondrial function. In addition, the increase of PFASs exposure was associated with the accumulation of key metabolites in kynurenine metabolism, which are involved in immune responses (Combined β coefficient of 0.60 [95% CI: 0.25-0.95, P = 0.001]). Moreover, alternations in PFASs-associated metabolites in mitochondrial and kynurenine metabolism were also correlated with clinical lab biomarkers for mitochondrial function (serum growth/differentiation factor-15) and immune activity (lymphocyte percentage), respectively.

Conclusion: Elevated exposure to PFASs was independently associated with an increased risk of COVID-19 infection. PFASs-associated metabolites were implicated in mitochondrial function and immune activity. Larger studies are needed to confirm our findings and further understand the underlying mechanisms of PFASs exposure in the pathogenesis of SARS-CoV2 infection.
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http://dx.doi.org/10.1016/j.envint.2021.106524DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7972714PMC
March 2021

Identification of serum biomarkers to predict pemetrexed/platinum chemotherapy efficacy for advanced lung adenocarcinoma patients by data-independent acquisition (DIA) mass spectrometry analysis with parallel reaction monitoring (PRM) verification.

Transl Lung Cancer Res 2021 Feb;10(2):981-994

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Thoracic Medical Oncology, Peking University Cancer Hospital & Institute, Beijing, China.

Background: Pemetrexed/platinum chemotherapy has been the standard chemotherapy regimen for lung adenocarcinoma patients, but the efficacy varies considerably.

Methods: To discover new serum biomarkers to predict the efficacy of pemetrexed/platinum chemotherapy, we analyzed 20 serum samples from advanced lung adenocarcinoma patients who received pemetrexed/platinum chemotherapy with the data-independent acquisition (DIA) quantitative mass spectrometry (MS).

Results: The 20 patients were categorized as "good response" [12 patients achieving partial response (PR)] and "poor response" [8 patients with progressive disease (PD)] groups. Altogether 23 significantly different expressed proteins were identified, which had relative ratios higher than 1.2 or lower than -0.83, with 7 proteins having an area under the curve (AUC) above 0.8. To further validate the DIA results, we used the parallel reaction monitoring (PRM) method to examine 16 candidate serum biomarkers in the study cohort of 20 patients and another cohort of 22 advanced lung adenocarcinoma patients (16 PR and 6 PD). Quantitative validation using PRM correlated well with the DIA results, and 10 promising proteins exhibited a similar up- or downregulation. It is worth noting that glutathione peroxidase 3 (GPX3) exhibits significant upregulation in the poor response group compared with the good response group, which was validated by both DIA and PRM methods.

Conclusions: Our study confirmed that combined DIA MS and PRM approaches were effective in identifying serum predictive biomarkers for advanced lung adenocarcinoma patients. Further studies are needed to explore the potential biological mechanism underlying these biomarkers.
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http://dx.doi.org/10.21037/tlcr-21-153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7947410PMC
February 2021

Contributions of epiphytic microbiota on the fermentation characteristics and microbial composition of ensiled six whole crop corn varieties.

J Appl Microbiol 2021 Mar 12. Epub 2021 Mar 12.

Institute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing, 210095, China.

Aims: The present study is aimed to reveal the variations in epiphytic microbial composition among six whole crop corn (WCC) varieties and their contributions on ensiling characteristics and microbial composition of WCC silage.

Methods And Results: Six WCC varieties (JS06, YS23, BS20, JS39, JS40 and JS26) were ensiled for 90 days. All WCC varieties were well fermented with low pH value (< 4.0) and high LA (73.6-124 g kg DM) concentration. Of six varieties, JS40 had the highest LA (124 g kg DM) concentration, which was supported by highest relative abundance of Lactobacillus. Pantoea was the most dominant epiphytic bacteria in all fresh WCC varieties; however the secondary dominant genera among six WCC were absolutely difference. Lactobacillus became predominant genus in 90-d silages except YS23. YS23 kept the more bacterial genus from fresh to 90-d silages than other silages, meanwhile Acinetobacter and Enterobacter was the dominant bacteria in YS23 silages.

Conclusions: Among 6 WCC varieties, JS40 silage had the highest LA. The variations in epiphytic microbiomes among fresh WCC affected terminal microbial community of 90-d silages. There were differences in fermentation characteristics among six WCC varieties, which might be partly attributed to variations in epiphytic microbiomes among fresh WCC.

Significance And Impact Of The Study: The study not only enriches the research on microbial communities of plant phyllosphere, but also provides theoretical basis for selecting WCC varieties and inoculants for the forage production.
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http://dx.doi.org/10.1111/jam.15064DOI Listing
March 2021

Fangchinoline exerts anticancer effects on colorectal cancer by inducing autophagy via regulation AMPK/mTOR/ULK1 pathway.

Biochem Pharmacol 2021 Apr 18;186:114475. Epub 2021 Feb 18.

Institute of Tissue Engineering and Stem Cells, Cancer Biotherapy Key Laboratory of Nanchong Nanchong Central Hospital, The Second Clinical Medical College, North Sichuan Medical College, Nanchong 637000, China. Electronic address:

Autophagy has become a promising target for cancer therapy. Fangchinoline (Fan) has been shown to exert anticancer effects in some types of cancers. However, the anticancer effects on colorectal cancer (CRC) and the underlying mechanisms have never been elucidated. More specifically, regulation of autophagy in CRC by Fan has never been reported before. In the present study, Fan was found to induce apoptosis and autophagic flux in the CRC cell lines HT29 and HCT116, which was reflected by the enhanced levels of LC3-II protein and p62 degradation, and the increased formation of autophagosomes and puncta formation by LC3-II. Meanwhile, combination with the early-stage autophagy inhibitor 3-methyladenine (3-MA) but not the late-stage autophagy inhibitor chloroquine (CQ) further increased Fan-induced cell death, which suggested the cytoprotective function of autophagy induced by Fan in both HT29 and HCT116 cells. Moreover, Fan treatment demonstrated a dose- and time-dependently increase in the phosphorylation of AMPK and decrease in the phosphorylation of mammalian target of rapamycin (mTOR) and ULK1, leading to the activation of the AMPK/mTOR/ULK1 signaling pathway. Furthermore, in the HT29 xenograft model, Fan inhibited tumor growth in vivo. These results indicate that Fan inhibited CRC cell growth both in vitro and in vivo and revealed a new molecular mechanism involved in the anticancer effect of Fan on CRC, suggesting that Fan is a potent autophagy inducer and might be a promising anticancer agent.
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http://dx.doi.org/10.1016/j.bcp.2021.114475DOI Listing
April 2021

Revealing the Dynamic Mechanism by Which Transferrin Promotes the Cellular Uptake of HAIYPRH Peptide-Conjugated Nanostructures by Force Tracing.

Mol Pharm 2021 03 31;18(3):1480-1485. Epub 2021 Jan 31.

School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, Changchun 130012, China.

The HAIYPRH (T7) peptide has been widely used as a ligand for constructing tumor-targeted nanodrug delivery systems since it can target the transferrin receptor (TfR) and then enter cells easily with the help of transferrin (Tf). However, the dynamic mechanism by which transferrin promotes the entry of T7-conjugated nanostructures into cells remains unclear. Herein, a force tracing technique based on atomic force microscopy (AFM) was used to track the ultrafast dynamic process of a T7-conjugated gold nanoparticle (AuNP-T7) entering a cell at the single-particle level in real time. Tf helped decrease the endocytosis force and increase the endocytosis speed of AuNP-T7 in A549 cells. However, Tf only increased the endocytosis speed of AuNP-T7 in HeLa cells. In contrast, in Vero cells without TfR overexpression, Tf decreased the endocytosis speed. This report provides important insights for redesigning and developing T7-conjugated nanodrug carriers in targeted nanodrug delivery systems.
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http://dx.doi.org/10.1021/acs.molpharmaceut.0c01119DOI Listing
March 2021

Occurrence and distribution of antimicrobial resistance genes in the soil of an industrial park in China: A metagenomics survey.

Environ Pollut 2021 Jan 8;273:116467. Epub 2021 Jan 8.

College of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China. Electronic address:

As zoned areas of industries, industrial parks have great impacts on the environment. Several studies have demonstrated that chemical compounds and heavy metals released from industrial parks can contaminate soil, water, and air. However, as an emerging pollutant, antimicrobial resistance genes (ARGs) in industrial parks have not yet been investigated. Here, we collected soil samples from 35 sites in an industrial park in China and applied a metagenomics strategy to profile the ARGs and virulence factors (VFs). We further compared the relative abundance of ARGs between the sites (TZ_31-35) located in a beta-lactam antimicrobial-producing factory and other sites (TZ_1-30) in this industrial park. Metagenomic sequencing and assembly generated 14, 383, 065 contigs and 17, 631, 051 open reading frames (ORFs). Taxonomy annotation revealed Proteobacteria and Actinobacteria as the most abundant phylum and class, respectively. The 32 pathogenic bacterial genera listed in the virulence factor database (VFDB) were all identified from the soil metagenomes in this industrial park. In total, 685,354 ARGs (3.89% of the ORFs) and 272,694 virulence factors (VFs) (1.55% of the ORFs) were annotated. These ARGs exhibited resistance to several critically important antimicrobials, such as rifampins, fluroquinolones, and beta-lactams. In addition, no significant difference in the relative abundance of ARGs was observed between sites TZ_31-35 and TZ_1-30, indicating that ARGs have already disseminated widely in this industrial park. The present study gave us a better understanding of the whole picture of the resistome and virulome in the soil of the industrial park and suggested that we should treat the industrial park as a whole in the surveillance and maintenance of ARGs.
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http://dx.doi.org/10.1016/j.envpol.2021.116467DOI Listing
January 2021

Dynamics of delivering aptamer targeted nano-drugs into cells.

J Mater Chem B 2021 01 13;9(4):952-957. Epub 2021 Jan 13.

School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, Changchun 130012, China.

A targeted nano-drug delivery system has provided great potential and benefits to the diagnosis and therapy of cancers. Cell entry is a critical step for taking effect of the targeted nano-drug. In this report, the dynamics of delivering a single aptamer targeted polyamindoamine-camptothecin-AS1411 (PAMAM-CPT-AS1411) nano-drug into cells was investigated using a force tracing technique based on atomic force microscopy. The results show that the specific interaction of AS1411 and nucleolin, which is overexpressed on cancer cells, enhances the efficiency of the PAMAM-CPT-AS1411 cell entry. Moreover, the specific interaction induced receptor-mediated endocytosis prolongs the duration and decreases the speed of a single PAMAM-CPT-AS1411 cell entry, which is helpful to understand the targeted nano-drugs prolonging the therapeutic drug level. However, the required force for PAMAM-CPT-AS1411 cell entry is not changed. This report will provide a novel and potential method for achieving the precise dynamics of targeted nano-drug delivery.
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http://dx.doi.org/10.1039/d0tb02527eDOI Listing
January 2021

Abundance and diversity of epiphytic microbiota on forage crops and their fermentation characteristic during the ensiling of sterile sudan grass.

World J Microbiol Biotechnol 2021 Jan 12;37(2):27. Epub 2021 Jan 12.

Institute of Ensiling and Processing of Grass, College of Agro-Grassland Science, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, China.

This study aimed to evaluate the effects of exogenous epiphytic microbiota inoculation on the fermentation quality and microbial community of sudan grass silage. Gamma irradiated sudan grass was ensiled with distilled water (STR), epiphytic microbiota of sudan grass (SUDm), forage sorghum (FSm), napier grass (NAPm) and whole crop corn (WCCm). The FSm inoculated silage have significantly lower lactic acid (LA) concentration and higher pH during early ensiling, while LA concentration gradually and significantly increased with the progression of ensiling and have lower pH in relation to other treatments for terminal silage. Inoculation of NAPm resulted in lower LA and higher acetic acid (AA) concentrations, higher pH, ammonia-N and dry matter losses for terminal silage, followed by SUDm silage. Inoculations of WCCm significantly increased LA production and pH decline during early ensiling and have higher LA and pH then NAPm and SUDm silages during final ensiling. The early fermentation of SUDm silage was dominated by genus of Pediococcus. The genera of Lactobacillus were predominant in WCCm and NAPm silages during 3 days of ensiling, while Weissella dominated initial microbial community of FS silage. The terminal silage of NAPm was dominated by Enterobacter and Rosenbergiella, while Enterobacter and Lactobacillus dominated terminal SUDm silage. The final silage of FSm was dominated by Lactobacillus, Weissella and Pediococcus, while Lactobacillus and Acetobacter dominated terminal WCCm silages. The results demonstrated that among the four forages the epiphytic microbiota from forage sorghum positively influenced the microbial community and fermentability of sudan grass silage.
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http://dx.doi.org/10.1007/s11274-020-02991-3DOI Listing
January 2021

Laser Powder Bed Fusion of Pure Tungsten: Effects of Process Parameters on Morphology, Densification, Microstructure.

Materials (Basel) 2020 Dec 31;14(1). Epub 2020 Dec 31.

The State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

Tungsten has been widely used in many industrial fields due to its excellent properties. However, owing to its characteristics of inherent brittleness at room temperature and high melting point, it is difficult to prepare tungsten parts with high complexity via traditional methods. In the present work, tungsten samples were prepared by laser powder bed fusion. The influence of each process parameter including laser power, scanning speed, and hatch spacing on the surface morphology, densification, and microstructure of tungsten samples was systematically investigated. The results showed that the use of the appropriate parameters, especially high laser power, can effectively improve the surface quality and obtain a dense surface. The tungsten samples with a relative density of 98.31% were obtained with optimized parameter combinations: a laser power of 300 W, scanning speed of 400 mm/s, and hatch spacing of 0.08 mm. Compared with scanning speed and hatch spacing, the laser power had a more obvious influence on the relative density. Additionally, for the grain morphology by microstructure inspection, elongated curved grains gradually transformed into fine straight columnar grains as the scanning speed increased. The hatch spacing would change the grain morphology slightly but had no significant effect on the grain size.
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http://dx.doi.org/10.3390/ma14010165DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7796442PMC
December 2020

VEGI downregulation is correlated with nodal metastasis and poor prognosis in lung adenocarcinoma.

Mol Clin Oncol 2021 Feb 9;14(2):25. Epub 2020 Dec 9.

Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China.

Although the incidence of lung cancer is increasing worldwide, the molecular mechanisms for its tumorigenesis, progression and prognosis remain unknown. As a member of the tumor necrosis factor superfamily, vascular endothelial growth inhibitor (VEGI) is involved in the development and progression of many malignant diseases. In the present study, the expression of VEGI and CD31 was examined via immunohistochemistry in non-small cell lung cancer (NSCLC) tissues obtained from 150 patients with NSCLC. The inhibitory effect of VEGI on tumor-associated blood vessel formation and growth was investigated by determining the relationship between VEGI protein expression and microvascular density (MVD). Prognostic significance was evaluated using the Kaplan-Meier method. VEGI expression was downregulated or lost in 68.7% (103/150) of patients with NSCLC, an effect that was more prevalent in adenocarcinoma (AC), 76.0% (57/75), than in squamous cell carcinoma, 61.3% (46/75). A significant negative correlation was indentified between VEGI expression and lymphovascular invasion (P=0.039) and lymph node metastasis (P=0.017) in AC tissue. Additionally, MVD was significantly lower in the VEGI-rich group compared with the VEGI-poor group. The downregulation of VEGI expression was also associated with poorer overall survival (P=0.011) in patients with AC. The present study therefore provides evidence that VEGI may be a new and effective prognostic marker of lung AC.
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http://dx.doi.org/10.3892/mco.2020.2187DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7739847PMC
February 2021

Prediction of the VeriStrat test in first-line therapy of pemetrexed-based regimens for advanced lung adenocarcinoma patients.

Cancer Cell Int 2020 Dec 9;20(1):590. Epub 2020 Dec 9.

Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/ Beijing), Department of Thoracic Medical Oncology, Peking University Cancer Hospital & Institute, 52 Fucheng Road, Haidian District, Beijing, 100142, China.

Background: Although advanced non-squamous non-small cell lung cancer (NSCLC) patients have significantly better survival outcomes after pemetrexed based treatment, a subset of patients still show intrinsic resistance and progress rapidly. Therefore we aimed to use a blood-based protein signature (VeriStrat, VS) to analyze whether VS could identify the subset of patients who had poor efficacy on pemetrexed therapy.

Methods: This study retrospectively analysed 72 advanced lung adenocarcinoma patients who received first-line pemetrexed/platinum or combined with bevacizumab treatment.

Results: Plasma samples from these patients were analysed using VS and classified into the Good (VS-G) or Poor (VS-P) group. The relationship between efficacy and VS status was further investigated. Of the 72 patients included in this study, 35 (48.6%) were treated with pemetrexed plus platinum and 37 (51.4%) were treated with pemetrexed/platinum combined with bevacizumab. Among all patients, 60 (83.3%) and 12 (16.7%) patients were classified as VS-G and VS-P, respectively. VS-G patients had significantly better median progression-free survival (PFS) (Unreached vs. 4.2 months; P < 0.001) than VS-P patients. In addition, the partial response (PR) rate was higher in the VS-G group than that in the VS-P group (46.7% vs. 25.0%, P = 0.212). Subgroup analysis showed that PFS was also significantly longer in the VS-G group than that in the VS-P group regardless of whether patients received chemotherapy alone or chemotherapy plus bevacizumab.

Conclusions: Our study indicated that VS might be considered as a novel and valid method to predict the efficacy of pemetrexed-based therapy and identify a subset of advanced lung adenocarcinoma patients who had intrinsic resistance to pemetrexed based regimens. However, larger sample studies are still needed to further confirm this result.
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http://dx.doi.org/10.1186/s12935-020-01662-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7724790PMC
December 2020

Effects of different additives on fermentation quality and aerobic stability of a total mixed ration prepared with local feed resources on Tibetan plateau.

Anim Sci J 2020 Jan;91(1):e13482

Institute of Ensiling and Processing of Grass, College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, China.

To improve the utilization efficiency of total mixed ration (TMR) on Tibetan plateau, the effects of different additives on fermentation quality and aerobic stability of the ensiled TMR prepared with local feed resources were studied. A total of 150 experimental silos were prepared in a completely randomized design to evaluate the following treatments: (a) control; (b) Lactobacillus buchneri; (c) acetic acid; (d) propionic acid; (e) 1,2-propanediol; and (f) 1-propanol. After 90 days of ensiling, silos were opened for fermentation quality and in vitro parameters analysis, and then subjected to an aerobic stability test for 14 days. The acetic acid, 1,2-propanediol and 1-propanol treatments increased (p < .05) pH and acetic acid content, and lowered (p < .05) the lactic acid production in comparison to control. There were no statistically significant differences in in vitro digestibility parameters among the treatments. Treatments of acetic acid, 1,2-propanediol and 1-propanol substantially improved the aerobic stability of the ensiled TMR, as indicated by almost unchanged pH and lactic acid contents throughout the aerobic exposure test. These results indicated that acetic acid, 1,2-propanediol and 1-propanol had no adverse effect on in vitro digestibility and could be effective additives for enhancing the aerobic stability of ensiled TMR prepared on Tibetan plateau.
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http://dx.doi.org/10.1111/asj.13482DOI Listing
January 2020

Degradation of norfloxacin wastewater using kaolin/steel slag particle electrodes: Performance, mechanism and pathway.

Chemosphere 2021 May 17;270:128652. Epub 2020 Oct 17.

College of Water Conservancy and Architecture Engineering, Shihezi University, Shihezi, 832000, Xinjiang, PR China.

In this work, kaolin/steel slag particle electrodes (KSPEs) were synthesized using a calcination method, and they were used to degrade norfloxacin (NOR) wastewater in three-dimensional (3D) reactor. Characterization methods used by KSPEs included SEM, XRF, XRD and BET. The effects of cell voltage, initial pH, KSPEs dosage and initial NOR concentration on NOR degradation were studied in the optimization experiment of operating parameters. The NOR degradation rate and COD removal rate can reach 96.02% and 93.45% under the optimal parameters within 30 min, and energy consumption is 0.99 kWh m. As a result, KSPEs shows excellent catalytic performance and cycling, and still has high electrocatalytic activity after 10 cycles. Finally, the degradation mechanism and degradation pathways of KSPEs to treat NOR are proposed.
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http://dx.doi.org/10.1016/j.chemosphere.2020.128652DOI Listing
May 2021

Enhancement of biomass conservation and enzymatic hydrolysis of rice straw by dilute acid-assisted ensiling pretreatment.

Bioresour Technol 2021 Jan 29;320(Pt B):124341. Epub 2020 Oct 29.

Institute of Ensiling and Processing of Grass, College of Agro-grassland Science, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

To reduce the cost of lignocellulosic pretreatment, rice straw was ensiled with dilute formic acid (FA, 0, 0.2, 0.4, and 0.6%) for 3, 6, 9, 15 and 30 days, and evaluated its effects on fermentation dynamics, lignocellulosic degradation and enzymatic hydrolysis. The results showed that the application of FA, especially at 0.6% level, reduced total fermentation losses of the resulting silages, as evidenced by low dry matter loss, ammonia nitrogen and ethanol content. Meanwhile, the 0.6% FA application promoted hemicellulose removal (232.41 vs 187.52 g/kg DM) and xylose production (0.35 vs 2.80 g/kg DM). The glucose yield and cellulose convertibility of rice straw increased after 30 days of ensiling, and further enhanced by the 0.6% FA application. In conclusion, the 0.6% FA-assisted ensiling pretreatment improved both biomass preservation, hemicellulose removal and enzymatic hydrolysis of rice straw, which is beneficial to the subsequent biofuel production chain.
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http://dx.doi.org/10.1016/j.biortech.2020.124341DOI Listing
January 2021

Cellulose nanocrystals/graphene oxide composite for the adsorption and removal of levofloxacin hydrochloride antibiotic from aqueous solution.

R Soc Open Sci 2020 Oct 14;7(10):200857. Epub 2020 Oct 14.

State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin, China Institute of Water Resources and Hydropower Research, Beijing, 100038, People's Republic of China.

Residual antibiotics in water are often persistent organic pollutants. The purpose of this study was to prepare a cellulose nanocrystals/graphene oxide composite (CNCs-GO) with a three-dimensional structure for the removal of the antibiotic levofloxacin hydrochloride (Levo-HCl) in water by adsorption. The scanning electron microscope, Fourier transform infrared (FT-IR), energy-dispersive spectroscopy, X-ray photoelectron spectroscopy and other characterization methods were used to study the physical structure and chemical properties of the CNCs-GO. The three-dimensional structure of the composite material rendered a high surface area and electrostatic attraction, resulting in increased adsorption capacity of the CNCs-GO for Levo-HCl. Based on the Box-Behnken design, the effects of different factors on the removal of Levo-HCl by the CNCs-GO were explored. The composite material exhibited good antibiotic adsorption capacity, with a removal percentage exceeding 80.1% at an optimal pH of 4, the adsorbent dosage of 1.0 g l, initial pollutant concentration of 10.0 mg l and contact time of 4 h. The adsorption isotherm was well fitted by the Sips model, and kinetics studies demonstrated that the adsorption process conformed to a quasi-second-order kinetics model. Consequently, the as-synthesized CNCs-GO demonstrates good potential for the effective removal of antibiotics such as levofloxacin hydrochloride from aqueous media.
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http://dx.doi.org/10.1098/rsos.200857DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7657919PMC
October 2020

The sinking behavior of micro-nano particulate matter for bisphenol analogues in the surface water of an ecological demonstration zone, China.

Environ Sci Process Impacts 2021 Feb;23(1):98-108

College of Water Conservancy and Architecture Engineering, Shihezi University, Shihezi 832000, Xinjiang, China.

Bisphenol analogues (BPs) have been widely used in industrial production as substitutes of bisphenol A (BPA). The demand and production of BPs are growing rapidly in China. In this study, the pollution levels and distribution characteristics of five BPs were investigated in surface water from rivers located in different land-use types of an ecological demonstration zone. All BPs were detected at least once in the traditionally dissolved phase, colloidal phase and suspended particulate matter (SPM) with the mean total concentrations of 465.1 ng L-1, 114.4 ng L-1 and 11.3 μg g-1 dry weight, respectively. BPA is the dominant BP in the traditionally dissolved phase and colloidal phase, with the mean contribution rates of 77.6% and 70.7%, respectively, followed by bisphenol F (BPF) and/or bisphenol S (BPS). The colloids as the important sinks of contaminants contributed 42.3% of bisphenol Z, 37.3% of BPF, 24.9% of BPA, 22.3% of BPAF and 18.4% of BPS in the traditionally dissolved phase. However, BPA alternatives are found primarily in the SPM, in which the contribution rate of BPA ranges from 0.6% to 48.1%, with the mean contribution of 12.4%. Based on BP concentrations in the traditionally dissolved phase, moderate ecological risk levels of BPA and BPF towards aquatic organisms were posed. Fish and/or algae are the most sensitive aquatic organisms, and hence chronic toxicological effects should not be ignored especially in fish.
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http://dx.doi.org/10.1039/d0em00366bDOI Listing
February 2021

Biomimetic Polyurethane 3D Scaffolds Based on Polytetrahydrofuran Glycol and Polyethylene Glycol for Soft Tissue Engineering.

Polymers (Basel) 2020 Nov 9;12(11). Epub 2020 Nov 9.

College of Materials, Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610059, Sichuan, China.

In this study, a novel polyurethane porous 3D scaffold based on polyethylene glycol (PEG) and polytetrahydrofuran glycol (PTMG) was developed by in situ polymerization and freeze drying. Aliphatic hexamethylene diisocyanate (HDI) as a nontoxic and safe agent was adopted to produce the rigid segment in polyurethane polymerization. The chemical structure, macrostructure, and morphology-as well as mechanical strength of the scaffolds-were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM), and tensile tests. The results show that the HDI can react mildly with hydroxyl (-OH) groups of PEG and PTMG, while gas foaming action caused by the release of CO occurred simultaneously in the reactive process, resulting in a uniform porous structure of PU scaffold. Moreover, the scaffolds were soaked in water and freeze dried to obtain higher porosity and more interconnective microstructures. The scaffolds have a porosity of over 70% and pore size from 100 to 800 μm. The mechanical properties increased with increasing PEG content, while the hydrophilicity increased as well. After immersion in simulated body fluid (SBF), the scaffolds presented a stable surface structure. The gas foaming/freezing drying process is an excellent method to prepare skin tissue engineering scaffold from PTMG/PEG materials with high porosity and good inter connectivity.
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http://dx.doi.org/10.3390/polym12112631DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7697348PMC
November 2020

Clinical features and factors leading to early recurrence of intussusception after saline reduction.

J Pak Med Assoc 2020 Oct;70(10):1727-1730

Department of Pediatric Surgery, Weifang Peoples Hospital, Weifang City, Shandong Province, China.

Objective: To investigate the clinical characteristics of early recurrent intussusception after ultrasound-guided saline reduction, and to explore the factors leading to early recurrence.

Methods: The retrospective observational case-control study was conducted at Weifang People's Hospital, Shandong, China, and comprised data from January 2015 to December 2017 related to paediatric intussusception patients aged 0-12 years who underwent ultrasound-guided saline enema reduction. The patients were divided into two recurrent and non-recurrent groups. Clinical characteristics of the patients with early recurrence were analysed. Factors compared between the groups were gender, age, onset season, onset-to-treatment time interval, blood in stool, fever, diarrhoea, abdominal pain and vomiting, weight and pathology. Data was analysed using SPSS 22.

Results: Of the 672 subjects, 86(13%) were patient with early recurrence while 586(87%) had no early recurrence and acted as controls. Among the patients, 70(81.4%) were aged 6-36 months. In 52(60.5%) patients, recurrence was once, and in 23(26.7%) twice. There were 141 episodes of intussusception; 24(17%) occurring in <12 hours, 85(60.2%) in 12-24 hours. Also, 5(6%) patients required surgery for reduction. Compared to the controls, the second quarter, heavier body weight and pathology were the factors leading to early recurrence of intussusceptions (p<0.05).

Conclusions: The second quarter, heavier body weight and pathological leading points were found to be factors leading to early recurrent intussusception.
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http://dx.doi.org/10.5455/JPMA.21744DOI Listing
October 2020

Mesenchymal stem cell-derived exosomal microRNA-136-5p inhibits chondrocyte degeneration in traumatic osteoarthritis by targeting ELF3.

Arthritis Res Ther 2020 10 27;22(1):256. Epub 2020 Oct 27.

Department of Orthopedics, The Second Hospital of Jilin University, No. 218, Ziqiang Street, Nanguan District, Changchun, 130041, Jilin Province, People's Republic of China.

Background: Emerging evidence suggests that microRNAs (miRs) are associated with the progression of osteoarthritis (OA). In this study, the role of exosomal miR-136-5p derived from mesenchymal stem cells (MSCs) in OA progression is investigated and the potential therapeutic mechanism explored.

Methods: Bone marrow mesenchymal stem cells (BMMSCs) and their exosomes were isolated from patients and identified. The endocytosis of chondrocytes and the effects of exosome miR-136-5p on cartilage degradation were observed and examined by immunofluorescence and cartilage staining. Then, the targeting relationship between miR-136-5p and E74-like factor 3 (ELF3) was analyzed by dual-luciferase report assay. Based on gain- or loss-of-function experiments, the effects of exosomes and exosomal miR-136-5p on chondrocyte migration were examined by EdU and Transwell assay. Finally, a mouse model of post-traumatic OA was developed to evaluate effects of miR-136-5p on chondrocyte degeneration in vivo.

Results: In the clinical samples of traumatic OA cartilage tissues, we detected increased ELF3 expression, and reduced miR-136-5p expression was determined. The BMMSC-derived exosomes showed an enriched level of miR-136-5p, which could be internalized by chondrocytes. The migration of chondrocyte was promoted by miR-136-5p, while collagen II, aggrecan, and SOX9 expression was increased and MMP-13 expression was reduced. miR-136-5p was verified to target ELF3 and could downregulate its expression. Moreover, the expression of ELF3 was reduced in chondrocytes after internalization of exosomes. In the mouse model of post-traumatic OA, exosomal miR-136-5p was found to reduce the degeneration of cartilage extracellular matrix.

Conclusion: These data provide evidence that BMMSC-derived exosomal miR-136-5p could promote chondrocyte migration in vitro and inhibit cartilage degeneration in vivo, thereby inhibiting OA pathology, which highlighted the transfer of exosomal miR-136-5p as a promising therapeutic strategy for patients with OA.
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http://dx.doi.org/10.1186/s13075-020-02325-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7590698PMC
October 2020

FN1 promotes chondrocyte differentiation and collagen production via TGF-β/PI3K/Akt pathway in mice with femoral fracture.

Gene 2021 Feb 22;769:145253. Epub 2020 Oct 22.

Department of Orthopedics, The Second Hospital of Jilin University, Changchun 130041, PR China. Electronic address:

Fibronectin (FN) functions as a potent stimulator of osteogenic differentiation, and bone fracture healing. In FN family, FN1 acts as an interactive protein gene product to mediate chondrocyte adhesion. However, its effect on fracture healing remains elusive. Therefore, we aimed to investigate the involvement of FN1 in fracture healing. Hard callus formations were found at fracture site with thicker periosteum in lateral cortical bone area outside the fracture site in model mice. The decreased number of osteogenic cells in the middle of the callus region and increased extracellular matrix were suggestive of successful induction. Immunoblotting and RT-qPCR revealed that expression of FN1 was increased in tissues of fracture mice. As displayed by Safranin-fast green staining hematoxylin-eosin staining, the overexpression of FN1 at fracture site promoted osteoid formation and chondrocyte differentiation. The stimulating role of FN1 in collagen production was evidenced by increased levels of Col2, Col1, ColX, Osteonectin, and Osteocalcin and enhanced BMD, BV, BV/TV and Tb.Th values verified by immunoblotting and immunohistochemical staining. Additionally, the upregulation of FN1 contributed to promoted TGF-β, c-Caspase-9/t-Caspase-9 ratio and NF-κB p65 protein expression as well as lowered p-PI3K/PI3K and p-AKT/AKT ratios, implying the positive correlation between FN1 and the TGF-β/PI3K/Akt signaling pathway. The key findings of the present study provided evidence indicating that overexpression of FN1 contributes to fracture healing by activation of the TGF-β/PI3K/Akt signaling pathway.
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http://dx.doi.org/10.1016/j.gene.2020.145253DOI Listing
February 2021

An improved multivariate model that distinguishes COVID-19 from seasonal flu and other respiratory diseases.

Aging (Albany NY) 2020 10 21;12(20):19938-19944. Epub 2020 Oct 21.

School of Medicine, University of California, San Diego, CA 92093, USA.

COVID-19 shared many symptoms with seasonal flu, and community-acquired pneumonia (CAP) Since the responses to COVID-19 are dramatically different, this multicenter study aimed to develop and validate a multivariate model to accurately discriminate COVID-19 from influenza and CAP. Three independent cohorts from two hospitals (50 in discovery and internal validation sets, and 55 in the external validation cohorts) were included, and 12 variables such as symptoms, blood tests, first reverse transcription-polymerase chain reaction (RT-PCR) results, and chest CT images were collected. An integrated multi-feature model (RT-PCR, CT features, and blood lymphocyte percentage) established with random forest algorism showed the diagnostic accuracy of 92.0% (95% CI: 73.9 - 99.1) in the training set, and 96. 6% (95% CI: 79.6 - 99.9) in the internal validation cohort. The model also performed well in the external validation cohort with an area under the receiver operating characteristic curve of 0.93 (95% CI: 0.79 - 1.00), an F1 score of 0.80, and a Matthews correlation coefficient (MCC) of 0.76. In conclusion, the developed multivariate model based on machine learning techniques could be an efficient tool for COVID-19 screening in nonendemic regions with a high rate of influenza and CAP in the post-COVID-19 era.
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http://dx.doi.org/10.18632/aging.104132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7655178PMC
October 2020

SDF-1 mediates mesenchymal stem cell recruitment and migration via the SDF-1/CXCR4 axis in bone defect.

J Bone Miner Metab 2021 Mar 20;39(2):126-138. Epub 2020 Oct 20.

Department of Geriatric Medicine, The Second Hospital of Jilin University, No. 218, Ziqiang Street, Nanguan District, Changchun, 130041, Jilin, People's Republic of China.

Introduction: Recent studies have indicated the potential of stem cell therapy in combination with cytokines to restore the bone repair via migration and homing of stem cells to the defected area. The present study aimed to investigate the mobilization and recruitment of mesenchymal stem cells (MSCs) in response to SDF-1.

Materials And Methods: Herein, the knockout rat model of the bone defect (BD) was treated with the induced membrane technique. Then, wild type Wistar rats and SDF-1-knockout rats were selected for the establishment of BD-induced membrane (BD-IM) models and bone-graft (BG) models. The number of MSCs was evaluated by flow cytometry, along with the expression pattern of the SDF-1/CXCR4 axis as well as osteogenic factors was identified by RT-qPCR and Western blot analyses. Finally, the MSC migration ability was assessed by the Transwell assay.

Results: Our data illustrated that in the induced membrane tissues, the number of MSCs among the BD-IM modeled rats was increased, whereas, a lower number was documented among BG modeled rats. Besides, we found that lentivirus-mediated over-expression of SDF-1 in BG modeled rats could activate the SDF-1/CXCR4 axis, mobilize MSCs into the defect area, and up-regulate the osteogenic proteins.

Conclusions: Collectively, our study speculated that up-regulation of SDF-1 promotes the mobilization and migration of MSCs through the activation of the SDF-1/CXCR4 signal pathway.
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http://dx.doi.org/10.1007/s00774-020-01122-0DOI Listing
March 2021

The Study of Reactive Ion Etching of Heavily Doped Polysilicon Based on HBr/O/He Plasmas for Thermopile Devices.

Materials (Basel) 2020 Sep 25;13(19). Epub 2020 Sep 25.

Key Laboratory of Microelectronics Devices & Integrated Technology, Institute of Microelectronics, Chinese Academy of Sciences, Beijing 100029, China.

Heavily doped polysilicon layers have been widely used in the fabrication of microelectromechanical systems (MEMS). However, the investigation of high selectivity, anisotropy, and excellent uniformity of heavily doped polysilicon etching is limited. In this work, reactive ion etching of undoped and heavily doped polysilicon-based hydrogen bromide (HBr) plasmas have been compared. The mechanism of etching of heavily doped polysilicon is studied in detail. The final results demonstrate that the anisotropy profile of heavily doped polysilicon can be obtained based on a HBr plasma process. An excellent uniformity of resistance of the thermocouples reached ± 2.11%. This technology provides an effective away for thermopile and other MEMS devices fabrication.
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http://dx.doi.org/10.3390/ma13194278DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7579393PMC
September 2020

Naphthoquinone-derivative as a synthetic compound to overcome the antibiotic resistance of methicillin-resistant S. aureus.

Commun Biol 2020 Sep 24;3(1):529. Epub 2020 Sep 24.

Department of Biological Sciences, Kent State University, Kent, OH, 44240, USA.

The treatment of Staphylococcus aureus (S. aureus) infections has become more difficult due to the emergence of multidrug resistance in the bacteria. Here, we report the synthesis of a lawsone (2-hydroxy-1,4-naphthoquinone)-based compound as an antimicrobial agent against methicillin-resistant S. aureus (MRSA). A series of lawsone-derivative compounds were synthesized by means of tuning the lipophilicity of lawsone and screened for minimum inhibitory concentrations against MRSA to identify a candidate compound that possesses a potent antibacterial activity. The identified lawsone-derivative compound exhibited significantly improved drug resistance profiles against MRSA compared to conventional antibiotics. The therapeutic efficacy of the compound was validated using murine models of wound infection as well as non-lethal systemic infection induced by MRSA. Our study further revealed the multifaceted modes of action of the compound, mediated by three distinctive mechanisms: (1) cell membrane damage, (2) chelation of intracellular iron ions, and (3) generation of intracellular reactive oxygen species.
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http://dx.doi.org/10.1038/s42003-020-01261-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7518446PMC
September 2020

Fasting blood glucose level is a predictor of mortality in patients with COVID-19 independent of diabetes history.

Diabetes Res Clin Pract 2020 Nov 10;169:108437. Epub 2020 Sep 10.

Department of Endocrinology, Renmin Hospital of Wuhan University, Wuhan, China. Electronic address:

Aim: No study elucidated the role of fasting blood glucose (FBG) level in the prognosis
of coronavirus disease 2019 (COVID-19).

Methods: This cohort study was conducted in a single center at Renmin Hospital of Wuhan University, Wuhan, China. Clinical laboratory, and treatment data of inpatients with laboratory-confirmed COVID-19 were collected and analyzed. Outcomes of patients with and without pre-existing diabetes were compared. The associations of diabetes history and/or FBG levels with mortality were analyzed. Multivariate cox regression analysis on the risk factors associated with mortality in patients with COVID-19 was performed.

Results: A total of 941 hospitalized patients with COVID-19 were enrolled in the study. There was a positive relationship between pre-existing diabetes and the mortality of patients who developed COVID-19 (21 of 123 [17.1%] vs 76 of 818 [9.3%]; P = 0.012). FBG ≥7.0 mmol/L was an independent risk factor for the mortality of COVID-19 regardless of the presence or not of a history of diabetes (hazard ratio, 2.20 [95% CI, 1.21-4.03]; P = 0.010).

Conclusions: We firstly showed FBG ≥7.0 mmol/L predicted worse outcome in hospitalized patients with COVID-19 independent of diabetes history. Our findings indicated screening FBG level is an effective method to evaluate the prognosis of patients with COVID-19.
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http://dx.doi.org/10.1016/j.diabres.2020.108437DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7482587PMC
November 2020

Preparation and In Vitro and In Vivo Antitumor Effects of VEGF Targeting Micelles.

Technol Cancer Res Treat 2020 Jan-Dec;19:1533033820957022

154454The Second Hospital of Jilin University, Nanguan District, Changchun, China.

Background: Doxorubicin (DOX) has antitumor effects mediated by cell viability inhibition and by inducing cellular apoptosis. However, it has limited use in clinical applications due to various factors such as hydrophobicity, dose-dependent toxicity effects on normal tissues, short cycle retention time, and low targeting ability. This study aims at enhancing hydrophilicity of DOX to restrict its toxic effects to within or around the tumor sites and also to improve its targeting ability to enhance antitumor efficiency.

Methods: Micelles composed of biodegradable poly (ethylene glycol)-poly (lactic acid) copolymers (PEG-PLA) were employed to deliver DOX via a self-assembly method and were coupled to VEGF antibodies. The morphology, size, and physical stability of PEG-PLA-DOX targeting VEGF micelles (VEGF-PEG-PLA-DOX micelles) were assessed. Then, the release ability of DOX from these micelles was monitored, and their drug loading capacity was calculated. MTT assay revealed the antitumor effect of VEGF-PEG-PLA-DOX micelles. Moreover, ROS release was measured to evaluate apoptotic effects of these nanoparticle micelles. therapeutic efficiencies of VEGF-PEG-PLA-DOX micelles on a lung cancer nude mouse model was evaluated.

Results: DOX-loaded micelles were obtained with a drug loading capacity of 12.2% and were monodisperse with 220 nm average diameter and a controlled DOX release for extended periods. In addition, VEGF-PEG-PLA-DOX micelles displayed a larger cell viability inhibitory effect as measured via MTT assays and greater cell apoptosis induction through ROS levels compared with PEG-PLA-DOX micelles or free DOX. Furthermore, VEGF-PEG-PLA-DOX micelles could improve antitumor effects of DOX by reducing tumor volume and weight.

Conclusions: VEGF-PEG-PLA-DOX micelles displayed a larger anti-tumor effect both in A549 cells and in an lung cancer nude mouse model compared with PEG-PLA-DOX micelles or free DOX, and hence they have potential clinical applications in human lung cancer therapy.
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http://dx.doi.org/10.1177/1533033820957022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7488921PMC
September 2020