Publications by authors named "Juncheng Liu"

44 Publications

Low Surface Roughness Graphene Oxide Film Reduced with Aluminum Film Deposited by Magnetron Sputtering.

Nanomaterials (Basel) 2021 May 28;11(6). Epub 2021 May 28.

Tianjin Key Laboratory of Advanced Fibers and Energy Storage, School of Material Science and Engineering, Tiangong University, Tianjin 300387, China.

Graphene film has wide applications in optoelectronic and photovoltaic devices. A novel and facile method was reported for the reduction of graphene oxide (GO) film by electron transfer and nascent hydrogen produced between aluminum (Al) film deposited by magnetron sputtering and hydrochloric acid (HCl) solution for only 5 min, significantly shorter than by other chemical reduction methods. The thickness of Al film was controlled utilizing a metal detection sensor. The effect of the thickness of Al film and the concentration of HCl solution during the reduction was explored. The optimal thickness of Al film was obtained by UV-Vis spectroscopy and electrical conductivity measurement of reduced GO film. Atomic force microscope images could show the continuous film clearly, which resulted from the overlap of GO flakes, the film had a relatively flat surface morphology, and the surface roughness reduced from 7.68 to 3.13 nm after the Al reduction. The film sheet resistance can be obviously reduced, and it reached 9.38 kΩ/sq with a high transmittance of 80% (at 550 nm). The mechanism of the GO film reduction by electron transfer and nascent hydrogen during the procedure was also proposed and analyzed.
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http://dx.doi.org/10.3390/nano11061428DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227777PMC
May 2021

Soybean-derived gma-miR159a alleviates colon tumorigenesis by suppressing TCF7/MYC in mice.

J Nutr Biochem 2021 06 8;92:108627. Epub 2021 Mar 8.

College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing, China. Electronic address:

Previous reports have shown that plant-derived microRNAs (miRNAs) regulate mammalian gene expression through dietary intake. Our prior study found that gma-miR159a, which is abundant in soybean, significantly inhibited the proliferation of colon cancer cells. In the current study, dietary gma-miR159a was utilized to study its anti-colon cancer function in azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colon cancer mice. Under processing conditions, gma-miR159a exhibited excellent stability in cooked soybean. In vitro, gma-miR159a suppressed the expression of the oncogene MYC downstream of the Wnt signaling pathway by targeting the TCF7 gene, significantly inhibiting the growth of colon cancer cells. The in vivo experiments showed that gma-miR159a and soybean RNA (total RNA extracted from soybean) significantly reduced tumor growth in AOM/DSS-induced colon cancer mice by gavage. This effect disappeared when anti-miR159a was present. In addition, gma-miR159a and soybean RNA significantly attenuated inflammation in colon cancer mice. These results showed that long-term dietary intake of soybean-derived gma-miR159a effectively prevented the occurrence of colon cancer and colitis, which provides novel evidence for the prevention function of soybean.
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http://dx.doi.org/10.1016/j.jnutbio.2021.108627DOI Listing
June 2021

Synthesis and Properties of Polyimide Silica Nanocomposite Film with High Transparent and Radiation Resistance.

Nanomaterials (Basel) 2021 Feb 24;11(3). Epub 2021 Feb 24.

School of Materials Science and Engineering, Tiangong University, Tianjin 300387, China.

In order to prepare flexible glass cover sheet materials suitable for space solar cells, fluorinated diamine 2,2'-bistrifluoromethyl benzidine (TFDB) and fluorinated dianhydride 4,4' (hexafluoroisopropyl) diphthalic dianhydride (6FDA) as the monomer, polyimide (PI)/SiO composite film was synthesized by in situ polymerization, and the influence of coupling agent and SiO nanoparticle content on the film structure and properties was studied. The results show that PI synthesized from fluorine-containing monomers has better light transmittance, and the highest transmittance can reach 91.4%. The average visible light transmittance of the composite film decreases with the increase of SiO content, and the transmittance of the film decreases less in the high-wavelength region and greatly decreases in the low-wavelength region. The tensile strength and elastic modulus of PI/SiO composite film increase with the increase of SiO content, first increase and then decrease, reaching the maximum when the content is 10%; while the elongation at break of the composite film gradually increases with the increase of SiO content reduce. The thermal stability of PI/SiO composite film increases with the increase of SiO content. The doping of nano-SiO significantly suppresses the influence of irradiation on the mechanical properties of the film.
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http://dx.doi.org/10.3390/nano11030562DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7995970PMC
February 2021

Metabolic Syndrome in Offspring of Parents with Metabolic Syndrome: A Meta-Analysis.

Obes Facts 2021 28;14(1):148-162. Epub 2021 Jan 28.

Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China,

Background: The metabolic syndrome (MetS) represents a clustering of risk factors for cardiovascular diseases that includes abdominal obesity, hypertension, dyslipidemia, and insulin resistance.

Objectives: The objective of this study was to reassess the parent-offspring association of MetS since the available findings are still controversial.

Methods: The Cochrane Library, PubMed, Embase, and Web of Science databases were searched to identify relevant articles. All studies comparing MetS status between the offspring of parents with MetS and offspring of parents without MetS were included in the analysis.

Results: A total of 9 studies met the inclusion criteria and they were analyzed. Offspring of at least 1 parent with MetS had a higher risk of MetS (OR 3.88, 95% CI 2.58-5.83, p < 0.001). Sons and daughters of fathers with MetS both had a higher risk of MetS (OR 2.31, 95% CI 1.70-3.12, p < 0.001, and OR 1.73, 95% CI 1.37-2.18, p < 0.001, respectively). Sons and daughters of mothers with MetS both had a higher risk of MetS (OR 1.95, 95% CI 1.37-2.76, p = 0.0002, and OR 1.91, 95% CI 1.54-2.35, p < 0.001, respectively).

Conclusion: This meta-analysis showed that there is a higher risk of MetS in the offspring of parents with MetS. However, there was no differential association of MetS according to gender and/or age of the offspring.
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http://dx.doi.org/10.1159/000513370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7983676PMC
July 2021

Nitride-Oxide-Metal Heterostructure with Self-Assembled Core-Shell Nanopillar Arrays: Effect of Ordering on Magneto-Optical Properties.

Small 2021 Feb 15;17(5):e2007222. Epub 2021 Jan 15.

School of Materials Engineering, Purdue University, West Lafayette, IN, 47907, USA.

Magneto-optical (MO) coupling incorporates photon-induced change of magnetic polarization that can be adopted in ultrafast switching, optical isolators, mode convertors, and optical data storage components for advanced optical integrated circuits. However, integrating plasmonic, magnetic, and dielectric properties in one single material system poses challenges since one natural material can hardly possess all these functionalities. Here, co-deposition of a three-phase heterostructure composed of a durable conductive nitride matrix with embedded core-shell vertically aligned nanopillars, is demonstrated. The unique coupling between ferromagnetic NiO core and atomically sharp plasmonic Au shell enables strong MO activity out-of-plane at room temperature. Further, a template growth process is applied, which significantly enhances the ordering of the nanopillar array. The ordered nanostructure offers two schemes of spin polarization which result in stronger antisymmetry of Kerr rotation. The presented complex hybrid metamaterial platform with strong magnetic and optical anisotropies is promising for tunable and modulated all-optical-based nanodevices.
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http://dx.doi.org/10.1002/smll.202007222DOI Listing
February 2021

CHIC Risk Stratification System for Predicting the Survival of Children With Hepatoblastoma: Data From Children With Hepatoblastoma in China.

Front Oncol 2020 18;10:552079. Epub 2020 Nov 18.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

Objective: The aim of this study was to compare the accuracy of the Children's Oncology Group (COG) risk stratification system to the Children's Hepatic tumor International Collaboration (CHIC) risk stratification system for predicting the prognosis of Chinese children with hepatoblastoma (HB).

Methods: Clinicopathological data of 86 patients diagnosed with HB between January 2014 and December 2017 were retrieved. The study endpoints were the 1- and 3-year overall survival (OS) and disease-free survival (DFS) were analyzed to evaluate the predictive value.

Results: The 1-, 3-year OS and DFS of the 86 patients were 86.0%, 76.3%, and 74.4%, 54.0%, respectively. Univariate analyses revealed that age at diagnosis had a significant role in prognosis for both OS and DFS, along with PRETEXT staging and metastasis at diagnosis. Multivariate analysis showed that metastasis at diagnosis (HR 3.628, 95% CI 1.404-9.378, P=0.008), PRETEXT staging system (HR 2.176, 95% CI 1.230-3.849, P=0.008) and age at diagnosis (HR 2.268, 95% CI 1.033-4.982, P=0.041) were independent factors for OS. For DFS, the independent factors were the PRETEXT staging system (HR 2.241, 95% CI 1.533-3.277, P<0.001) and age at diagnosis (HR 1.792, 95% CI 1.018-3.154, P=0.043). Both COG and CHIC risk stratification systems could effectively predict the prognosis of children with HB for OS. For DFS, the CHIC risk stratification system was more effective. In addition, the CHIC risk stratification system had a higher c-index (OS 0.743, DFS 0.730), compared to the COG risk stratification system (OS 0.726, DFS 0.594).

Conclusion: Age at diagnosis played a significant role in prognosis. Compared to the COG risk stratification system, the CHIC risk stratification system was superior in predicting the survival of Chinese children with HB.
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http://dx.doi.org/10.3389/fonc.2020.552079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7708347PMC
November 2020

3D Hybrid Trilayer Heterostructure: Tunable Au Nanorods and Optical Properties.

ACS Appl Mater Interfaces 2020 Oct 22;12(40):45015-45022. Epub 2020 Sep 22.

School of Materials Engineering, Purdue University, West Lafayette, Indiana 47907, United States.

Engineering plasmonic nanostructures from three dimensions (3D) is very attractive toward controllable and tunable nanophotonic components and devices. Herein, Au-based trilayer heterostructures composed of a dielectric spacer sandwiched by hybrid Au-TiN vertically aligned nanocomposite (VAN) nanoplasmonic claddings are demonstrated with a broad range of geometries and property tuning. Two types of spacer layers, that is, a pure dielectric BaTiO layer and a hybrid plasmonic Au-BaTiO VAN layer, contribute to the tuning of the Au nanorod dimension. Such geometrical variations of Au nanostructures originate from the surface energy and lattice strain tuned by the spacer layers. Optical measurements and numerical simulations suggest the change of the localized surface plasmon resonance which is strongly affected by the tailored Au nanorods as either separated or channeled. The uniaxial dielectric tensors suggest a tunable hyperbolic property affected by such a metal-insulator-metal trilayer stack. The complex 3D heterostructures offer additional tuning parameters and design flexibilities in hybrid plasmonic metamaterials toward potential applications in light harvesting, sensing, and nanophotonic devices.
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http://dx.doi.org/10.1021/acsami.0c14937DOI Listing
October 2020

PDI-Based Hexapod-Shaped Nonfullerene Acceptors for the High-Performance As-Cast Organic Solar Cells.

ACS Appl Mater Interfaces 2020 Aug 10;12(33):37409-37417. Epub 2020 Aug 10.

Key Laboratory of Energy Conversion and Storage Materials, College of Chemistry, Beijing Normal University, Beijing 100875, P. R. China.

Three hexapod-shaped PDI-hexamers (PSM1, PSM2, and PSM3) with a diphenylmethylene-bridged triphenylamine (TPA) core and six peripheral PDI subunits have been designed and synthesized. The influence of different peripheral PDI subunits on the morphology and crystallinity of acceptors is investigated. Distinctly different from the previously reported PDI trimers with a TPA core, which exhibit amorphous morphologies, these hexapod-shaped acceptors display improved crystallinities and photophysical properties. Our studies have shown that PSM3 with six peripheral thiophene-fused PDI subunits gives the best result. The as-cast blend films of PBDB-T and PSM3, which possess appropriate phase separation and higher crystallinity, show high and balanced charge mobilities. As expected, OSCs with PBDB-T:PSM3 as the active layer achieve the highest power conversion efficiency of 6.71% among these three acceptors, which is the highest one in TPA-based acceptors and one of the best for the as-cast OSCs based on PDI derivatives.
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http://dx.doi.org/10.1021/acsami.0c11159DOI Listing
August 2020

Exchange Bias in a LaSrMnO/NiO Heterointerface Integrated on a Flexible Mica Substrate.

ACS Appl Mater Interfaces 2020 Sep 20;12(35):39920-39925. Epub 2020 Aug 20.

School of Materials Engineering, Purdue University, West Lafayette, Indiana 47907, United States.

Flexible electronics integrating spintronics are of great potential in the areas of lightweight and flexible personal electronics. The integration of ferromagnetic and other functional oxides on flexible mica substrates is crucial for the proposed computer technology. In this work, we demonstrate the successful integration of a ferromagnetic-antiferromagnetic nanocomposite of LaSrMnO (LSMO)/NiO with unique perpendicular exchange bias properties on a flexible mica substrate. Utilization of multiple sets of buffer layers has been attempted to overcome the large mismatch between the film and the substrate and to achieve high-quality nanocomposite growth on mica. Exchange bias of ∼200 and ∼140 Oe for the applied magnetic field perpendicular and parallel to the film surface, respectively, has been achieved and attributed to the strongly coupled vertical ferromagnetic/antiferromagnetic interfaces. Such nanocomposite thin films exhibit excellent structural robustness and reliability under a cyclic bending test. This work demonstrates the enormous potential of integrating complex two-phase multifunctional oxides on mica for future flexible wearable personal devices.
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http://dx.doi.org/10.1021/acsami.0c12935DOI Listing
September 2020

Wheat germ-derived peptide ADWGGPLPH abolishes high glucose-induced oxidative stress via modulation of the PKCζ/AMPK/NOX4 pathway.

Food Funct 2020 Aug;11(8):6843-6854

College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210046, China.

This study explores the antioxidative effect of a specific wheat germ-derived peptide on high glucose-induced oxidative stress in vascular smooth muscle cells (VSMCs) and the underlying mechanisms. The peptide ADWGGPLPH was identified by LC-MS/MS. The effects of this peptide on the production of ROS and the expression of oxidative stress signaling proteins in VSMCs were determined. STZ-induced mice were utilized to confirm the anti-oxidative and anti-diabetic cardiovascular disease effects of this peptide in vivo. The results showed that ADWGGPLPH significantly prevented high glucose-induced cell proliferation, decreased intracellular ROS generation, stimulated AMPK activity, inhibited the PKCζ, AKT and Erk1/2 phosphorylation, and suppressed NOX4 protein expression. In addition, ADWGGPLPH enhanced the antioxidant abilities and attenuated inflammatory cytokine generation in STZ-induced diabetic mice. Therefore, ADWGGPLPH prevents high glucose-induced oxidative stress in VSMCs by modulating the PKCζ/AMPK/NOX4 pathway.
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http://dx.doi.org/10.1039/d0fo01229gDOI Listing
August 2020

Soybean-derived miRNAs specifically inhibit proliferation and stimulate apoptosis of human colonic Caco-2 cancer cells but not normal mucosal cells in culture.

Genomics 2020 09 11;112(5):2949-2958. Epub 2020 May 11.

College of Food Science and Engineering, Collaborative Innovation Center for Modern Grain Circulation and Safety, Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210046, China. Electronic address:

MicroRNAs (miRNAs) are important regulators of gene expression in eukaryotes. Studies have shown that plant-derived miRNAs can be absorbed through diets and regulate gene expression in mammals. Although soybean-derived miRNAs have been reported, their biological functions are still unclear. In this study, we found that soybean-derived small RNAs (sRNAs) significantly inhibited the proliferation and stimulated the apoptosis of Caco-2 cells. Bioinformatics analysis indicated that the target gene set of soybean miRNAs was extensively enriched in cancer pathways. Besides, we obtained 8 target genes, including Transcription factor 7 (TCF7), associated with colon cancer through prediction. Further studies showed that gma-miR159a inhibited the proliferation of Caco-2 cells and played an important role in the inhibitory effect of sRNAs by inhibiting TCF7 protein, which are upregulated in colon cancer cells but not normal mucosal cells in culture. These findings provide a novel molecular mechanism of soybean-derived miRNAs for potential application in tumor prevention.
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http://dx.doi.org/10.1016/j.ygeno.2020.05.011DOI Listing
September 2020

Efficient Organic Solar Cells Based on Non-Fullerene Acceptors with Two Planar Thiophene-Fused Perylene Diimide Units.

ACS Appl Mater Interfaces 2020 Mar 24;12(9):10746-10754. Epub 2020 Feb 24.

Key Laboratory of Energy Conversion and Storage Materials, College of Chemistry, Center for Advanced Quantum Studies, Department of Physics and Applied Optics, Beijing Area Major Laboratory, Beijing Normal University, Beijing 100875, P. R. China.

We designed and synthesized two non-fullerene acceptors (CDT-TFP and C8X-TFP), which comprise a central 4-cyclopenta[2,1-:3,4-']dithiophene (CDT) as the bridge and two thiophene-fused perylene diimide (TFP) units. The bulky side chains, such as the 4-hexylphenyl side chains, on the CDT bridge can effectively prevent the acceptor molecules from forming large aggregates, and the π-π stacking of the terminal planar TFP units can form effective electron transport pathways when blending with the donor polymers. These non-fullerene acceptors are used to fabricate organic solar cells (OSCs) by blending with the regioregular middle bandgap polymer reg-PThE. The as-cast devices based on reg-PThE:CDT-TFP show the best power conversion efficiency (PCE) of 8.36% with a of 1.10 V, of 12.43 mA cm, and an FF of 61.4%, whereas the analogue perylene diimide (PDI) dimers (CDT-PDI) that comprise two PDI units bridged with a CDT unit show only a 2.59% PCE with a of 0.92 V, of 6.82 mA cm, and an FF of 41.5%. Our results have demonstrated that the non-fullerene acceptors comprising planar PDI units can achieve excellent photovoltaic performance and provide meaningful guidelines for the design of PDI-based non-fullerene electron acceptors for efficient OSCs.
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http://dx.doi.org/10.1021/acsami.9b22927DOI Listing
March 2020

Dopamine D receptor protected against hyperglycemia-induced endothelial dysfunction via PI3K /eNOS pathway.

Biochem Biophys Res Commun 2019 10 22;518(3):554-559. Epub 2019 Aug 22.

Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Henan, PR China. Electronic address:

Hyperglycemia-induced endothelial dysfunction is generally believed to be the basis of diabetic vascular complications. Dopamine receptors is known to play an important protective role in diabetes. However, the protective effect of dopamine receptors against hyperglycemia-induced endothelial damage in diabetic rats is still unknown. In the present study, we established a cell model of hyperglycemia-induced endothelial dysfunction by treating human umbilical vein endothelial cells (HUVEC) with high glucose. MTT and lactate dehydrogenase assays results showed that high glucose treatment significantly reduced the cell viability and down-regulated dopamine D receptor. Pre-treatment with PD168077, a specific D receptor agonist, greatly improved endothelial cell viability and decreased apoptosis. Furthermore, pharmacological inhibition of phosphoinositide 3-kinase (PI3K) and endothelial nitric oxide synthase (eNOS) eliminated the protective effect of D receptor against endothelial injury. More importantly, the expression level of D receptor was also dramatically down-regulated in the arterial endothelium of rats with streptozotocin-(STZ)-induced diabetes, and the STZ-induced impairment of acetylcholine-induced vasodilation was reversed by activation of D receptor. In conclusion, our results indicated that dopamine D receptor protected against hyperglycemia-induced endothelial dysfunction via the PI3K/eNOS pathway, which may provide a novel strategy in the treatment of diabetes.
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http://dx.doi.org/10.1016/j.bbrc.2019.08.080DOI Listing
October 2019

Transcriptome Analysis of Acid-Responsive Genes and Pathways Involved in Polyamine Regulation in Iron Walnut.

Genes (Basel) 2019 08 10;10(8). Epub 2019 Aug 10.

College of Forestry, Sichuan Agricultural University, Huimin Road 211 in Wenjiang District, Chengdu 611130, China.

We reported changes in the co-regulated mRNA expression in iron walnut () in response to soil pH treatments and identified mRNAs specific to acidic soil conditions. Phenotypic and physiological analyses revealed that iron walnut growth was greater for the pH 4-5 and pH 5-6 treatments than for the pH 3-4 and pH 6-7 treatments. A total of 2768 differentially expressed genes were detected and categorized into 12 clusters by Short Time-series Expression Miner (STEM). The 994 low-expression genes in cluster III and 255 high-expression genes in cluster X were classified as acid-responsive genes on the basis of the relationships between phenotype, physiology, and STEM clustering, and the two gene clusters were analyzed by a maximum likelihood (ML) evolutionary tree with the greatest log likelihood values. No prominent sub-clusters occurred in cluster III, but three occurred in cluster X. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that acid-responsive genes were related primarily to arginine biosynthesis and the arginine/proline metabolism pathway, implying that polyamine accumulation may enhance iron walnut acid stress tolerance. Overall, our results revealed 1249 potentially acid-responsive genes in iron walnut, indicating that its response to acid stress involves different pathways and activated genes.
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http://dx.doi.org/10.3390/genes10080605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723594PMC
August 2019

Fluorescence In Situ Hybridization (FISH) Analysis of the Locations of the Oligonucleotides 5S rDNA, (AGGGTTT), and (TTG) in Three Genera of Oleaceae and Their Phylogenetic Framework.

Genes (Basel) 2019 05 17;10(5). Epub 2019 May 17.

College of Forestry, Sichuan Agricultural University, Wenjiang District, Chengdu 611130, China.

We report the cytogenetic map for a collection of species in the Oleaceae, and test similarities among the karyotypes relative to their known species phylogeny. The oligonucleotides 5S ribosomal DNA (rDNA), (AGGGTTT), and (TTG) were used as fluorescence in situ hybridization (FISH) probes to locate the corresponding chromosomes in three Oleaceae genera: , , , and × . Forty-six small chromosomes were identified in four species. (AGGGTTT) signals were observed on almost all chromosome ends of four species, but (AGGGTTT) played no role in distinguishing the chromosomes but displayed intact chromosomes and could thus be used as a guide for finding chromosome counts. (TTG) and 5S rDNA signals discerned several chromosomes located at subterminal or central regions. Based on the similarity of the signal pattern (mainly in number and location and less in intensity) of the four species, the variations in the 5S rDNA and (TTG) distribution can be ordered as < × < < . Variations have observed in the three genera. The molecular cytogenetic data presented here might serve as a starting point for further larger-scale elucidation of the structure of the Oleaceae genome, and comparison with the known phylogeny of Oleaceae family.
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http://dx.doi.org/10.3390/genes10050375DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562466PMC
May 2019

First report of bicolour FISH of and reveals interspecific differences and co-localization of (AGGGTTT) and rDNA 5S in .

Hereditas 2019 25;156:13. Epub 2019 Apr 25.

College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang District, Chengdu City, 611130 China.

Background: consists of approximately 500 species and is the largest genus in Berberidaceae. Most species lack cytological data, and bicolour fluorescence in situ hybridization (FISH) has never been performed on . In this work, a karyotype of , an alpine species obtained from an altitude of 3600 m in Wolong National Nature Reserve, China, was analysed and compared with Schneid. via FISH using oligonucleotide telomere probes for (AGGGTTT) and 5S rDNA (41 bp) for the first time.

Results: belonged to cytotype 2A and had the karyotype formula 2n = 2x = 28 = 26 m + 2 sm (2SAT). The mitotic metaphase chromosome lengths ranged from 1.82 ± 0.04 μm to 2.75 ± 0.00 μm. Clear (AGGGTTT) signals were detected at two telomeres in every chromosome and were co-localized with 5S rDNA at the terminal regions of the long arms in the 6 pair of chromosomes. One pair of (AGGGTTT) sites was localized in the satellites of the 7 pair of chromosomes, which are the only submetacentric chromosomes in this species. Totally 28 chromosomes with one pair of satellited chromosomes were observed in . This species had four 5S rDNA signals with two weak signals at the end of long arms in the 5 pair of chromosomes and another two strong signals detected in the interstitial region close to the end of short arms in the 6 pair of chromosomes. Each large signal consisted of two smaller signals with secondary constrictions around them.

Conclusions: FISH physical mapping of suggested that (AGGGTTT) and rDNA 5S co-localize at the 6 pair of chromosomes. The density, location and number difference of 5S rDNA loci indicated structural differences among the chromosomes between and Our results provide information that may contribute to future studies on the physical assembly of the genome and the evolution of rDNA and telomere FISH patterns in .
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http://dx.doi.org/10.1186/s41065-019-0088-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6485065PMC
December 2019

FISH analysis of Zanthoxylum armatum based on oligonucleotides for 5S rDNA and (GAA).

Genome 2018 Sep 1;61(9):699-702. Epub 2018 Aug 1.

College of Forestry, Sichuan Agricultural University, Huimin Road 211, Wenjiang District 611130, Chengdu City, China.

Fluorescence in situ hybridization (FISH) using oligonucleotide probes for (GAA) (18 bp) and ribosomal DNA (rDNA) (5S rDNA, 41 bp) was applied to analyse Zanthoxylum armatum. (GAA) loci were detected on the pericentromeric regions of five chromosome pairs, and 5S rDNA loci were also detected on the pericentromeric regions of another two chromosome pairs. The densities and locations of (GAA) and 5S rDNA signals varied between individual chromosomes. High-intensity (GAA) signals were detected at the centromeres of two large and two smaller metacentric chromosomes. Relatively strong (GAA) signals were detected at the centromeres of two relatively small metacentric chromosomes, although strong 5S rDNA signals were detected at the centromeres of two additional smaller metacentric chromosomes. Weak (GAA) signals were detected at the centromeres of four large metacentric chromosomes, whereas weak 5S rDNA signals were detected at the centromeres of two smaller metacentric chromosomes. The remaining chromosomes exhibited no signals. Zanthoxylum armatum had 2n = ∼128. The lengths of the mitotic metaphase chromosomes ranged from 1.22 to 2.34 μm. Our results provide information that may be beneficial for future cytogenetic studies and could contribute to the physical assembly of the Zanthoxylum genome.
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http://dx.doi.org/10.1139/gen-2018-0009DOI Listing
September 2018

The Prognostic Value of CD8 and CD45RO T Cells Infiltration and Beclin1 Expression Levels for Early Postoperative Cholangitis of Biliary Atresia Patients after Kasai Operation.

J Korean Med Sci 2018 Jul 7;33(30):e198. Epub 2018 Jun 7.

Department of Pediatric Surgery, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.

Background: Postoperative cholangitis is a common but severe complication after Kasai portoenterostomy for biliary atresia (BA). This study aimed to identify its prognostic factors.

Methods: Two sets of liver paraffin-embedded tissue samples were collected from BA patients who received Kasai portoenterostomy (n = 25 and n = 31, respectively). Patients were divided into non-cholangitis and cholangitis groups. The infiltration of CD4, CD8, CD45RO, CD68 cells and expression of Beclin1 were quantitatively evaluated in immunohistochemical analysis.

Results: Cholangitis group had a significantly lower CD8 T cell infiltration but a higher CD45RO cell infiltration, and a lower Beclin1 level than non-cholangitis group (all < 0.01). Multivariate logistic regression analysis indicated that infiltration of CD8 cells (odds ratio [OR], 0.112; 95% confidence interval [CI], 0.022-0.577) and CD45RO cells (OR, 3.88; 95% CI, 1.37-11.03), and Beclin1 level (OR, 0.088; 95% CI, 0.018-0.452) were independent influence factors for early postoperative cholangitis. Receiver operating characteristic (ROC) analysis showed that area under ROC curve (AUROC) values for CD8 cells, CD45RO cells and Beclin1 were 0.857, 0.738 and 0.900, respectively.

Conclusion: Our findings demonstrated the CD8 cells, CD45RO cells and Beclin1 level possessed the prognostic value for early postoperative cholangitis following Kasai operation, which may be helpful to develop new prevention and treatment strategies for postoperative cholangitis.
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http://dx.doi.org/10.3346/jkms.2018.33.e198DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6052325PMC
July 2018

Microstructure and Mechanical Properties of Al₂O₃/Er₃Al₅O Binary Eutectic Ceramic Prepared by Bridgman Method.

Materials (Basel) 2018 Mar 30;11(4). Epub 2018 Mar 30.

School of Materials Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, China.

Directionally solidified Al₂O₃/Er₃Al₅O (EAG) eutectic ceramic was prepared via vertical Bridgman method with high-frequency induction heating. The effects of the growth rate on the microstructure and mechanical properties of the solidified ceramic were investigated. The experimental results showed that there were no pores or amorphous phases in the directionally solidified Al₂O₃/EAG eutectic ceramic. Al₂O₃ phase was embedded in the EAG matrix phase, and the two phases were intertwined with each other to form a typical binary eutectic "hieroglyphic" structure. With the increase of growth rate, the phase size and spacing of the solidified Al₂O₃/EAG ceramic both decreased, and the growth rate and phase spacing satisfied the ² ≈ 60 formula of Jackson-Hunt theory. The cross section microstructure of the solidified ceramic always exhibited an irregular eutectic growth, while the longitudinal section microstructure presented a directional growth. The mechanical properties of the solidified ceramic gradually increased with the increase of growth rate, and the maximum hardness and fracture toughness could reach 21.57 GPa and 2.98 MPa·m respectively. It was considered that the crack deflection and branching could enhance the toughness of the solidified ceramic effectively.
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http://dx.doi.org/10.3390/ma11040534DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5951418PMC
March 2018

Single-axis rotation/azimuth-motion insulation inertial navigation control system with FOGs.

Opt Express 2017 Dec;25(25):30956-30975

Rotation modulation technology could effectively improve the accuracy of the inertial navigation system (INS) by compensating for the biases of the inertial sensors automatically. However, the carrier angular motion and rotation control error could reduce the rotation modulation effect and then decrease the navigation accuracy. To address this problem, for the single-axis rotation INS, a novel rotation control scheme is presented. The control scheme employs the fiber optic gyros to control the inertial measurement unit (IMU) rotation angular velocity so that the INS with both rotation modulation and azimuth motion insulation functions. Furthermore, in order to reduce the control error, this study adopts two ways: optimizing the control strategy and shortening the delay time. The former way is to control the IMU rotating about the z-axis of the platform frame with respect to the navigation frame, rather than the up-axis of the navigation frame. The latter way is to apply interrupt mode rather than inquiry mode to complete the data transfer between the navigation and the control processors. The simulation and experimental results demonstrate that: the proposed method would not only realize the rotation modulation of the biases of the inertial sensors, but also achieve the insulation of the azimuth motion. The steady-state control error of the control system is less than 10" and the overshoot control error is less than 50". Compared to the traditional SRINS, the navigation position error in the single-axis rotation/azimuth-motion insulation INS could reduce 50% in some navigation application.
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http://dx.doi.org/10.1364/OE.25.030956DOI Listing
December 2017

Genomic relationships among sixteen species of Avena based on (ACT) trinucleotide repeat FISH.

Genome 2018 Jan 30;61(1):63-70. Epub 2017 Nov 30.

c Triticeae Research Institute, Sichuan Agricultural University, Huimin Road 211, Wenjiang District 611130, Chengdu City, Sichuan Province, China.

Knowledge of the locations of repeat elements could be very important in the assembly of genome sequences and their assignment to physical chromosomes. Genomic and species relationships among 16 species were investigated using fluorescence in situ hybridization (FISH) with the Am1 and (ACT) probes. The Am1 oligonucleotide probe was particularly enriched in the C genomes, whereas the (ACT) trinucleotide repeat probe showed a diverse distribution of hybridization patterns in the A, AB, C, AC, and ACD genomes but might not be present in the B and D genomes. The hybridization pattern of Avena sativa was very similar to that of A. insularis, indicating that this species most likely originated from A. insularis as a tetraploid ancestor. Although the two FISH probes failed to identify relationships of more species, this proof-of-concept approach opens the way to the use of FISH probes in assigning other signature elements from genomic sequence to physical chromosomes.
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http://dx.doi.org/10.1139/gen-2017-0132DOI Listing
January 2018

A novel bioremediation strategy for petroleum hydrocarbon pollutants using salt tolerant Corynebacterium variabile HRJ4 and biochar.

J Environ Sci (China) 2016 Sep 4;47:7-13. Epub 2016 Mar 4.

College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.

The present work aimed to develop a novel strategy to bioremediate the petroleum hydrocarbon contaminants in the environment. Salt tolerant bacterium was isolated from Dagang oilfield, China and identified as Corynebacterium variabile HRJ4 based on 16S rRNA gene sequence analysis. The bacterium had a high salt tolerant capability and biochar was developed as carrier for the bacterium. The bacteria with biochar were most effective in degradation of n-alkanes (C16, C18, C19, C26, C28) and polycyclic aromatic hydrocarbons (NAP, PYR) mixture. The result demonstrated that immobilization of C. variabile HRJ4 with biochar showed higher degradation of total petroleum hydrocarbons (THPs) up to 78.9% after 7-day of incubation as compared to the free leaving bacteria. The approach of this study will be helpful in clean-up of petroleum-contamination in the environments through bioremediation process using eco-friendly and cost effective materials like biochar.
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http://dx.doi.org/10.1016/j.jes.2015.12.023DOI Listing
September 2016

Incidence of biliary atresia associated congenital malformations: A retrospective multicenter study in China.

Asian J Surg 2017 Nov 7;40(6):429-433. Epub 2016 Jul 7.

Department of Surgery, Shenzhen Children's Hospital, Shenzhen 518026, China.

Background: Some patients with biliary atresia (BA) have associated anomalies. Our study aimed to investigate the incidence of BA-associated malformations in mainland China, and compare the results with those reported in the Western literature.

Methods: Clinical data were collected retrospectively from five medical centers in mainland China. BA patients were diagnosed and confirmed by laparotomy with intraoperative cholangiography and liver biopsy. Cases were divided into isolated type BA and BA with associated anomalies, including polysplenia, situs inversus, intestinal malrotation, and cardiovascular anomalies.

Results: A total of 851 BA patients were recruited from Tianjin, Beijing, Wuhan, Guangzhou, and Shenzhen. Patients were grouped as follows: Type I, 13 cases (1.5%); Type II, five cases (0.6%); Type III, 833 cases (97.9%). Forty-two (4.94%) patients had 54 associated congenital abnormalities. The intra-abdominal anomalies included polysplenia (n = 4, 1 fusion between liver and spleen), situs inversus (n = 2), and intestinal malrotation (n = 3). The cardiovascular anomalies included atrial septal defect and ventricular septal defect (n = 29), patent foramen ovale (n = 1), patent ductus arteriosus (n = 4), and other cardiac malformations (n = 3, including coronary sinus dilation, left superior vena cava, Tetralogy of Fallot).

Conclusion: Our data showed that spleen anomaly is not as common as reported in the Western literature. The difference may suggests different genetic and environmental risk factors for BA.
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http://dx.doi.org/10.1016/j.asjsur.2016.04.003DOI Listing
November 2017

Removal of aqueous perfluorooctanoic acid (PFOA) using starch-stabilized magnetite nanoparticles.

Sci Total Environ 2016 08 18;562:191-200. Epub 2016 Apr 18.

Environmental Engineering Program, Department of Civil Engineering, Auburn University, Auburn, AL 36849, USA. Electronic address:

Fully stabilized magnetite (Fe3O4) nanoparticles were prepared with a water-soluble starch as a stabilizer and tested for removal of aqueous perfluorooctanoic acid (PFOA). The presence of starch at ≥0.2wt% can fully stabilize 0.1g/L as Fe of the Fe3O4 nanoparticles. The particle stabilization technique resulted in 2.4 times higher PFOA uptake. Fourier transform infrared spectra suggested that the main PFOA removal mechanism was inner-sphere complexation. Batch kinetic experiments revealed that the starch-stabilized nanoparticles facilitated a rapid PFOA uptake with a sorption equilibrium time of 30min, and the sorption process followed a pseudo-second-order kinetic model. The Langmuir model was able to well interpret the adsorption isotherm, with a maximum adsorption capacity of 62.5mg/g. Increasing pH from 4.7 to 9.6 led to a sharp increase (by 2.6 times) in PFOA uptake. The presence of 12mg/L humic acid inhibited PFOA uptake by 96%, while effect of ionic strength (CaCl2=0-2mmol/L) was negligible. The nanoparticles significantly reduced the biological toxicity of PFOA. The results demonstrated promise of starch-stabilized Fe3O4 nanoparticles as a "green" adsorbent for effective removal of PFOA in soil and groundwater.
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http://dx.doi.org/10.1016/j.scitotenv.2016.03.100DOI Listing
August 2016

Cyanidin 3-O-β-Glucoside Ameliorates Ethanol-Induced Acute Liver Injury by Attenuating Oxidative Stress and Apoptosis: The Role of SIRT1/FOXO1 Signaling.

Alcohol Clin Exp Res 2016 03;40(3):457-66

Department of Rheumatism , Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Background: The aim of this study was to examine the effects of Cyanidin 3-O-β-glucoside (C3G) on ethanol (EtOH)-induced acute liver injury in mice as well as in cultured hepatic cells exposed to EtOH, with a focus on the involvement of Silent Mating Type Information Regulation 2 Homolog 1 (SIRT1)/Forkhead fox-O-1 (FOXO1) signaling pathway, and to explore the underlying molecular mechanisms.

Methods: C57BL/6 adolescent male mice were given EtOH via intraperitoneal injection for 2 consecutive days, and the changes in the livers were detected via hematoxylin-eosin staining. The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured by biochemical methods. Protein expression of SIRT1, FOXO1, acetylated FOXO1 (ac-FOXO1), GRP78, p-eukaryotic initiation factor-2 (eIF2α), and apoptosis (p-JNK, p-c-Jun, and Bax) parameters was determined by Western blot. Reactive oxygen species (ROS) was detected by flow cytometry. Human hepatocytes Chang cell line was used to assay cell apoptosis by Annexin V and propidium iodide. In addition, mRNA levels of SIRT1, tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and monocyte chemoattractant protein 1 in liver tissues were detected by real-time polymerase chain reaction.

Results: This study demonstrated that C3G (10 mg/kg) administration diminished EtOH-induced acute liver injury compared to control group, as evidenced by the significant decreases in ALT and AST levels. Pretreatment with C3G exerted anti-inflammatory effects as indicated by the decreased TNF-α and IL-6 levels, as well as decreased inflammatory foci and ballooning cells in liver tissue. The lessened hepatic injury was associated with enhanced SIRT1 protein expression and activity by C3G in vitro and in vivo. C3G treatment also provoked significant attenuation of endoplasmic reticulum stress parameters (GRP78, p-eIF2α), which was consistent with reduced levels of both p-c-Jun and Bax. Interestingly, EX527 inhibitor did not affect the protective function of C3G on alcohol-induced cell apoptosis. Moreover, alcohol exposure increased ROS level and decreased ac-FOXO1, while C3G intervention reversed this abnormality, and this may be related to SIRT1 activity by C3G.

Conclusions: Anthocyanin C3G has significant potency in antioxidant, anti-inflammatory, and anti-apoptotic effects on hepatocytes exposed to EtOH by modulating the SIRT1/FOXO1 signaling pathway. Our findings illustrate a novel and definitive therapeutic action of C3G and represent an economically feasible therapeutic intervention to treat alcoholic liver disease.
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http://dx.doi.org/10.1111/acer.12982DOI Listing
March 2016

Photocatalytic degradation of commercially sourced naphthenic acids by TiO2-graphene composite nanomaterial.

Chemosphere 2016 Apr 11;149:328-35. Epub 2016 Feb 11.

Tianjin Academy of Environmental Sciences, Tianjin 300191, China.

Naphthenic acids (NAs) are a major contributor to the toxicity in oil sands process-affected water (OSPW), which is produced by hot water extraction of bitumen. NAs are extremely difficult to be degraded due to its complex ring and side chain structure. Photocatalysis is recognized as a promising technology in the removal of refractory organic pollutants. In this work, TiO2-graphene (P25-GR) composites were synthesized by means of solvothermal method. The results showed that P25-GR composite exhibited better photocatalytic activity than pure P25. The removal efficiency of naphthenic acids in acid solution was higher than that in neutral and alkaline solutions. It was the first report ever known on the photodegradation of NAs based on graphene, and this process achieved a higher removal rate than other photocatalysis degradation of NAs in a shorter reaction time. LC/MS analysis showed that macromolecular NAs (carbon number 17-22, z value -2) were easy to be degraded than the micromolecular ones (carbon number 11-16, z value -2). Furthermore, the reactive oxygen species that play the main role in the photocatalysis system were studied. It was found that holes and ·OH were the main reactive species in the UV/P25-GR photocatalysis system. Given the high removal efficiency of refractory organic pollutants and the short degradation time, photodegradation based on composite catalysts has a broad and practical prospect. The study on the photodegradation of commercially sourced NAs may provide a guidance for the degradation of OSPW NAs by this method.
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http://dx.doi.org/10.1016/j.chemosphere.2016.01.074DOI Listing
April 2016

A timesaving, low-cost, high-yield method for the synthesis of ultrasmall uniform graphene oxide nanosheets and their application in surfactants.

Nanotechnology 2016 Feb 16;27(5):055601. Epub 2015 Dec 16.

State Key Laboratory of Separation Membranes and Membrane Processes, School of Material Science and Engineering, Tianjin Polytechnic University, Tianjin 300387, People's Republic of China.

Graphene oxide nanosheets (GONSs) with a lateral size less than 100 nm have attracted more and more attention for their wide range of potential applications, from bionanotechnology and nanobiomedicine to surfactants. However, at present GONSs are commonly prepared from graphite nanofibers or graphite nanopowders which are both expensive. Here, a timesaving, low-cost, high-yield method is proposed for preparing ultrasmall uniform GONSs with an average lateral size of ∼30 nm, utilizing common graphite powder as the raw material in the absence of a strong acid. The obtained GONSs are able to disperse single-walled carbon nanotubes (SWCNTs) effectively, and the dispersion could withstand high-speed centrifugation. Consequently, GONSs could indeed serve as a superior surfactant for the dispersion of SWCNTs, and the dispersion could be further applied in electronics, as the GONSs may be further reduced to reduced GONSs or graphene nanosheets.
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http://dx.doi.org/10.1088/0957-4484/27/5/055601DOI Listing
February 2016

Sam68 is a novel marker for aggressive neuroblastoma.

Onco Targets Ther 2013 2;6:1751-60. Epub 2013 Dec 2.

Department of Pediatric Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, People's Republic of China.

Background: Neuroblastoma (NB) is the most common solid extracranial tumor in children. However, the molecular mechanism and progression of NB is largely unknown, and unfortunately, the prognosis is poor. Src-associated in mitosis with a molecular weight of 68 kDa (Sam68) is associated with carcinogenesis and neurogenesis. The present study aimed to investigate the clinical and prognostic significance of Sam68 in NB.

Methods: The expression of Sam68 in immortalized normal epithelial cells, NB cell lines, and in four cases of paired NB tissue and adjacent normal tissue from the same patient was examined using Western blotting, reverse transcription-polymerase chain reaction (PCR) and real-time reverse transcription-PCR. The proliferation of NB cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Furthermore, Sam68 protein expression was analyzed in 90 NB cases characterized as clinicopathological using immunohistochemistry. Statistical analyses were applied to evaluate the diagnostic value and associations of Sam68 with clinical parameters.

Results: Western blotting and reverse transcription-PCR showed that the expression level of Sam68 was markedly higher in NB cell lines than in the immortalized normal epithelial cells at both messenger RNA and protein levels. The MTT assay revealed that Sam68 expression supported proliferation of NB cells. Sam68 expression levels were significantly up-regulated in tumor tissues in comparison to the matched adjacent normal tissues from the same patient. Sam68 protein level was positively correlated with clinical stage (P<0.001), tumor histology (P<0.001), and distant metastasis (P=0.029). Patients with higher Sam68 expression had shorter overall survival time, whereas those with lower tumor Sam68 expression had longer survival time.

Conclusion: Our results suggest that Sam68 expression is associated with neuroblastoma progression and may represent a novel and valuable predictor for prognostic evaluation of neuroblastoma patients.
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http://dx.doi.org/10.2147/OTT.S52643DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3855102PMC
December 2013

The report of sustained low-efficiency dialysis (SLED) treatment in fifteen patients of severe snakebite.

Cell Biochem Biophys 2014 May;69(1):71-4

Department of Nephrology, The First Hospital of JingZhou, Yangtze University, Hangkongstreet 8, Jingzhou, 434000, Hubei, China,

To investigate the therapeutic efficacy of sustained low-efficiency dialysis (SLED) in severe snakebite patients. Fifteen patients of severe snakebite was treated with SLED from July 2005 to August 2009 were included in the study. Central venous access was established in all patients. SLED was administered using Dialog(+) dialyzer (B. Braun, Germany). SLED sessions were 6-12 h in duration at a blood flow rate of 200 ml/min and a dialysate flow rate of 300 ml/min. Heparin or low molecular weight heparin was used as anticoagulant. Biochemical indicators, APACHE II scores before and after SLED, and clinical outcomes were evaluated. The levels of serum creatinine, glutamic-oxaloacetic transaminase, glutamic-pyruvic transaminase, creatine kinase isozyme MB, and creatine kinase were significantly lower than the level before SLED (P < 0.05); the level of cholinesterase was significantly higher after SLED (P < 0.01); the APACHE II score before SLED was 14.1 ± 3.8, but decreased significantly to 7.9 ± 1.4, 6.2 ± 1.1, and 4.2 ± 0.8 on days 1, 2, and 7 after SLED, respectively (P < 0.01). Three patients died on days 1, 3, and 4 after SLED, respectively. The remaining twelve patients were either cured or showed improvement at the time of discharge. The survival rate was 80 % where as mortality was 20 %. SLED may be an effective treatment option in severe snakebite patients. It can reduce mortality, thereby, resulting in increased survival rates.
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http://dx.doi.org/10.1007/s12013-013-9768-4DOI Listing
May 2014

Malignant renal angiomyolipoma with metastases in a child.

Int J Surg Pathol 2014 Apr 22;22(2):160-6. Epub 2013 Aug 22.

1Sun Yat-sen University Cancer Center, Guangzhou, China.

The biological character of epithelioid angiomyolipoma (EAML) remains controversial and little is known about EAML in children. We present a case of a 7-year-old boy with abdominal distention, diagnosed as EAML. Under microscopy, epithelioid cells were observed, with eosinophilic or slightly eosinophilic cytoplasm and nuclear pleomorphism, thick-walled blood vessels, and some cells that were differentiated to smooth muscle cells or fat cells were easily observed. Immunochemical staining showed that Melan-A and HMB45 were positive. The tumor presented highly aggressive biobehavior. Furthermore, we reviewed and analyzed cases of diagnosed EAML in our hospital and those reported in the literature. Renal EAML (10/17) was most common, and the EAML of 3 of 17 patients metastasized to other organs.
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http://dx.doi.org/10.1177/1066896913497395DOI Listing
April 2014