Publications by authors named "Jun-jie Yang"

68 Publications

Cobalt-catalyzed chemoselective dehydrogenation through radical translocation under visible light.

Chem Sci 2022 Jul 15;13(26):7947-7954. Epub 2022 Jun 15.

State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University Lanzhou 730000 China

The transformations that allow the direct removal of hydrogen from their corresponding saturated counterparts by the dehydrogenative strategy are a dream reaction that has remained largely underexplored. In this report, a straightforward and robust cobaloxime-catalyzed photochemical dehydrogenation strategy intramolecular HAT is described for the first time. The reaction proceeds through an intramolecular radical translocation followed by the cobalt assisted dehydrogenation without needing any other external photosensitizers, noble-metals or oxidants. With this approach, a series of valuable unsaturated compounds such as α,β-unsaturated amides, enamides and allylic and homoallylic sulfonamides were obtained in moderate to excellent yields with good chemo- and regioselectivities, and the synthetic versatility was demonstrated by a range of transformations. And mechanistic studies of the method are discussed.
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http://dx.doi.org/10.1039/d2sc02291eDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258329PMC
July 2022

[Research status and prospects of integration of habitat processing and processing of Chinese medicinal decoction pieces].

Zhongguo Zhong Yao Za Zhi 2022 May;47(10):2565-2571

School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China.

The integration of habitat processing and processing of Chinese medicinal decoction pieces(hereinafter referred to as "integration") has changed the traditional processing mode and can ensure the quality of Chinese medicinal decoction pieces from the source. This paper introduced the background of integration from the connotation and denotation of integration, relevant policies and regulations, and variety development. The present situation of integration was analyzed from the existing problems and current research progress, and the development suggestions were proposed. It is considered that although the integration is in line with the development trend of the industry with the advantages of improving the quality and standardizing the management of decoction pieces, there are still some problems, such as the lack of variety selection principles and production technical specifications, imperfect quality control stan-dards in the production process, and inadequate integration of standards and supervision. Therefore, it is suggested to determine the integrated variety selection principles and variety range as soon as possible, establish relevant technical specifications, improve quality control standards in the production process, and strengthen policy guidance and supervision to promote the healthy and orderly development of integration.
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http://dx.doi.org/10.19540/j.cnki.cjcmm.20211228.601DOI Listing
May 2022

Lipoprotein(a) is associated with coronary atheroma progression: analysis from a serial coronary computed tomography angiography study.

J Geriatr Cardiol 2021 Dec;18(12):996-1007

Department of Cardiology, the Sixth Medical Centre, Chinese PLA General Hospital, Beijing, China.

Background: Lipoprotein(a) [Lp(a)] has been closely related to coronary atherosclerosis and might affect perivascular inflammation due to its proinflammatory properties. However, there are limited data about Lp(a) and related perivascular inflammation on coronary atheroma progression. Therefore, this study aimed to investigate the associations between Lp(a) and the perivascular fat attenuation index (FAI) with coronary atheroma progression detected by coronary computed tomography angiography (CCTA).

Methods: Patients who underwent serial CCTA examinations without a history of revascularization and with available data for Lp(a) within one month before or after baseline and follow-up CCTA imaging scans were considered to be included. CCTA quantitative analyses were performed to obtain the total plaque volume (TPV) and the perivascular FAI. Coronary plaque progression (PP) was defined as a ≥ 10% increase in the change of the TPV at the patient level or the presence of new-onset coronary atheroma lesions. The associations between Lp(a) or the perivascular FAI with PP were examined by multivariate logistic regression.

Results: A total of 116 patients were ultimately enrolled in the present study with a mean CCTA interscan interval of 30.80 ± 13.50 months. Among the 116 patients (mean age: 53.49 ± 10.21 years, males: 83.6%), 32 patients presented PP during the follow-up interval. Lp(a) levels were significantly higher among PP patients than those among non-PP patients at both baseline [15.80 (9.09-33.60) mg/dL 10.50 (4.75-19.71) mg/dL, = 0.029] and follow-up [20.60 (10.45-34.55) mg/dL 8.77 (5.00-18.78) mg/dL, = 0.004]. However, there were no differences in the perivascular FAI between PP group and non-PP group at either baseline or follow-up. Multivariate logistic regression analysis showed that elevated baseline Lp(a) level (OR = 1.031, 95% CI: 1.005-1.058, = 0.019) was an independent risk factor for PP after adjustment for other conventional variables.

Conclusions: Lp(a) was independently associated with coronary atheroma progression beyond low-density lipoprotein cholesterol and other conventional risk factors. Further studies are warranted to identify the inflammation effect exhibited as the perivascular FAI on coronary atheroma progression.
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http://dx.doi.org/10.11909/j.issn.1671-5411.2021.12.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782765PMC
December 2021

Two pairs of epimers and three undescribed diterpenoids from .

Nat Prod Res 2021 Oct 7:1-8. Epub 2021 Oct 7.

The MOE Key Laboratory of Standardization of Chinese Medicines, and SATCM Key Laboratory of New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, P. R. China.

A phytochemical investigation on the petroleum ether partition of the whole plant of , yield seven new compounds: one phytanes diterpenoid (2,6,10) 14-keto-2,6,10-trimethyl pentadeca-2,6,10-trien-1-carboxylic acid (), five clerodane diterpenoids: paniculatins A-E (, /, /), one abietane diterpenoid: -uncinatone (), together with 12 known compounds. Their structures were elucidated on the basis of 1D and 2D Nuclear Magnetic Resonance (NMR_, Infrared Radiation (IR), and mass spectroscopic data. Compound , and showed weak selective cytotoxic activity of 11 human cancer cell lines.
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http://dx.doi.org/10.1080/14786419.2021.1986494DOI Listing
October 2021

Diterpenoid glycosides from the flower of Trollius chinensis Bunge and their nitric oxide inhibitory activities.

Bioorg Chem 2021 11 30;116:105312. Epub 2021 Aug 30.

The MOE Key Laboratory of Standardization of Chinese Medicines and SATCM Key Laboratory of New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica (ICMM), Shanghai University of Traditional Chinese Medicine (SHUTCM), PR China; Shanghai R&D Center for Standardization of Chinese Medicines, Shanghai 201203, PR China. Electronic address:

Trolliusditerpenosides A-Q (1-17), seventeen new labdane-diterpenoid glycosides, were isolated from the dried flowers of Trollius chinensis Bunge, a plant that has been commonly used as both an anti-inflammatory folk medicine and a healthcare tea for its therapeutic and anti-viral and antibacterial properties. Their structures were corroborated via comprehensive spectroscopic analysis, ECD calculations, and single-crystal X-ray diffraction analysis. Furthermore, the inhibitory activities on lipopolysaccharide (LPS)-induced NO production in RAW 264.7 macrophages of all compounds (1-17) were evaluated in vitro. Compounds 3, 6, 7, and 11 displayed significant inhibitory activities against NO production, with IC values ranging from 1.6 ± 0.1 to 14.4 ± 0.2 μM. In addition, compounds 3, 6, 7, and 11 all down-regulated the mRNA expression of iNOS, COX-2, and IL-1β in RAW 264.7 cells mediated by LPS. These findings not only support the chemical context of genus Trollius but also the exploration of new chemical entities with pharmacological significance from this genus.
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http://dx.doi.org/10.1016/j.bioorg.2021.105312DOI Listing
November 2021

From a Designer Drug to the Discovery of Selective Cannabinoid Type 2 Receptor Agonists with Favorable Pharmacokinetic Profiles for the Treatment of Systemic Sclerosis.

J Med Chem 2021 01 31;64(1):385-403. Epub 2020 Dec 31.

Drug Discovery Unit, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, 500 Dongchuan Road, Shanghai 200241, P. R. China.

Synthetic cannabinoids, as exemplified by SDB-001 (), bind to both CB1 and CB2 receptors and exert cannabimimetic effects similar to (-)--Δ-tetrahydrocannabinol, the main psychoactive component present in the cannabis plant. As CB1 receptor ligands were found to have severe adverse psychiatric effects, increased attention was turned to exploiting the potential therapeutic value of the CB2 receptor. In our efforts to discover novel and selective CB2 receptor agonists, was selected as a starting point for hit molecule identification and a class of 1-pyrazole-3-carboxamide derivatives were thus designed, synthesized, and biologically evaluated. Systematic structure-activity relationship investigations resulted in the identification of the most promising compound as a selective CB2 receptor agonist with favorable pharmacokinetic profiles. Especially, treatment significantly attenuated dermal inflammation and fibrosis in a bleomycin-induced mouse model of systemic sclerosis, supporting that CB2 receptor agonists might serve as potential therapeutics for treating systemic sclerosis.
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http://dx.doi.org/10.1021/acs.jmedchem.0c01023DOI Listing
January 2021

Downregulation of T7 RNA polymerase transcription enhances pET-based recombinant protein production in Escherichia coli BL21 (DE3) by suppressing autolysis.

Biotechnol Bioeng 2021 01 18;118(1):153-163. Epub 2020 Sep 18.

Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.

Escherichia coli BL21 (DE3) is an excellent and widely used host for recombinant protein production. Many variant hosts were developed from BL21 (DE3), but improving the expression of specific proteins remains a major challenge in biotechnology. In this study, we found that when BL21 (DE3) overexpressed glucose dehydrogenase (GDH), a significant industrial enzyme, severe cell autolysis was induced. Subsequently, we observed this phenomenon in the expression of 10 other recombinant proteins. This precludes a further increase of the produced enzyme activity by extending the fermentation time, which is not conducive to the reduction of industrial enzyme production costs. Analysis of membrane structure and messenger RNA expression analysis showed that cells could underwent a form of programmed cell death (PCD) during the autolysis period. However, blocking three known PCD pathways in BL21 (DE3) did not completely alleviate autolysis completely. Consequently, we attempted to develop a strong expression host resistant to autolysis by controlling the speed of recombinant protein expression. To find a more suitable protein expression rate, the high- and low-strength promoter lacUV5 and lac were shuffled and recombined to yield the promoter variants lacUV5-1A and lac-1G. The results showed that only one base in lac promoter needs to be changed, and the A at the +1 position was changed to a G, resulting in the improved host BL21 (DE3-lac1G), which resistant to autolysis. As a consequence, the GDH activity at 43 h was greatly increased from 37.5 to 452.0 U/ml. In scale-up fermentation, the new host was able to produce the model enzyme with a high rate of 89.55 U/ml/h at 43 h, compared to only 3 U/ml/h achieved using BL21 (DE3). Importantly, BL21 (DE3-lac1G) also successfully improved the production of 10 other enzymes. The engineered E. coli strain constructed in this study conveniently optimizes recombinant protein overexpression by suppressing cell autolysis, and shows great potential for industrial applications.
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http://dx.doi.org/10.1002/bit.27558DOI Listing
January 2021

Gut microbiota and diabetes: From correlation to causality and mechanism.

World J Diabetes 2020 Jul;11(7):293-308

Microbiome-X, Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, School of Medicine and Engineering, Key Laboratory of Big Data-Based Precision Medicine, Beihang University, Beijing 100191, China.

In this review, we summarize the recent microbiome studies related to diabetes disease and discuss the key findings that show the early emerging potential causal roles for diabetes. On a global scale, diabetes causes a significant negative impact to the health status of human populations. This review covers type 1 diabetes and type 2 diabetes. We examine promising studies which lead to a better understanding of the potential mechanism of microbiota in diabetes diseases. It appears that the human oral and gut microbiota are deeply interdigitated with diabetes. It is that simple. Recent studies of the human microbiome are capturing the attention of scientists and healthcare practitioners worldwide by focusing on the interplay of gut microbiome and diabetes. These studies focus on the role and the potential impact of intestinal microflora in diabetes. We paint a clear picture of how strongly microbes are linked and associated, both positively and negatively, with the fundamental and essential parts of diabetes in humans. The microflora seems to have an endless capacity to impact and transform diabetes. We conclude that there is clear and growing evidence of a close relationship between the microbiota and diabetes and this is worthy of future investments and research efforts.
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http://dx.doi.org/10.4239/wjd.v11.i7.293DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7415231PMC
July 2020

Increasing rates of long-term nitrogen deposition consistently increased litter decomposition in a semi-arid grassland.

New Phytol 2021 01 9;229(1):296-307. Epub 2020 Sep 9.

Erguna Forest-Steppe Ecotone Research Station, CAS Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China.

The continuing nitrogen (N) deposition observed worldwide alters ecosystem nutrient cycling and ecosystem functioning. Litter decomposition is a key process contributing to these changes, but the numerous mechanisms for altered decomposition remain poorly identified. We assessed these different mechanisms with a decomposition experiment using litter from four abundant species (Achnatherum sibiricum, Agropyron cristatum, Leymus chinensis and Stipa grandis) and litter mixtures representing treatment-specific community composition in a semi-arid grassland under long-term simulation of six different rates of N deposition. Decomposition increased consistently with increasing rates of N addition in all litter types. Higher soil manganese (Mn) availability, which apparently was a consequence of N addition-induced lower soil pH, was the most important factor for faster decomposition. Soil C : N ratios were lower with N addition that subsequently led to markedly higher bacterial to fungal ratios, which also stimulated litter decomposition. Several factors contributed jointly to higher rates of litter decomposition in response to N deposition. Shifts in plant species composition and litter quality played a minor role compared to N-driven reductions in soil pH and C : N, which increased soil Mn availability and altered microbial community structure. The soil-driven effect on decomposition reported here may have long-lasting impacts on nutrient cycling, soil organic matter dynamics and ecosystem functioning.
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http://dx.doi.org/10.1111/nph.16854DOI Listing
January 2021

Probiotics and fructo-oligosaccharide intervention modulate the microbiota-gut brain axis to improve autism spectrum reducing also the hyper-serotonergic state and the dopamine metabolism disorder.

Pharmacol Res 2020 07 17;157:104784. Epub 2020 Apr 17.

Shandong Children's Microbiome Center, Research Institute of Pediatrics, Qilu Children's Hospital of Shandong University, Jinan, Shandong, China. Electronic address:

The prevalence of autism spectrum disorders (ASD) is increasing, but its etiology remains elusive and hence an effective treatment is not available. Previous research conducted on animal models suggests that microbiota-gut-brain axis may contribute to ASD pathology and more human research is needed. This study was divided into two stages,.At the discovery stage, we compared the differences in gut microbiota profiles (using 16S rRNA sequencing), fecal SCFAs (using GC-MS) and plasma neurotransmitters (using UHPLC-MS/MS) of 26 children with ASD and 24 normal children. All 26 children with ASD participated in the intervention stage, and we measured the gut microbiota profiles, SCFAs and neurotransmitters before and after probiotics + FOS (n = 16) or placebo supplementation (n = 10). We found that gut microbiota was in a state of dysbiosis and significantly lower levels of Bifidobacteriales and Bifidobacterium longum were observed at the discovery stage in children with ASD. An increase in beneficial bacteria (Bifidobacteriales and B. longum) and suppression of suspected pathogenic bacteria (Clostridium) emerged after probiotics + FOS intervention, with significant reduction in the severity of autism and gastrointestinal symptoms. Compared to children in the control group, significantly lower levels of acetic acid, propionic acid and butyric acid were found, and a hyperserotonergic state (increased serotonin) and dopamine metabolism disorder (decreased homovanillic acid) were observed in children with ASD. Interestingly, the above SCFAs in children with autism significantly elevated after probiotics + FOS intervention and approached those in the control group. In addition, our data demonstrated that decreased serotonin and increased homovanillic acid emerged after probiotics + FOS intervention. However, the above-mentioned changes did not appear in the placebo group for ASD children. Probiotics + FOS intervention can modulate gut microbiota, SCFAs and serotonin in association with improved ASD symptoms, including a hyper-serotonergic state and dopamine metabolism disorder.
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http://dx.doi.org/10.1016/j.phrs.2020.104784DOI Listing
July 2020

Improvement of lithium anode deterioration for ameliorating cyclabilities of non-aqueous Li-CO batteries.

Nanoscale 2020 Apr 2;12(15):8385-8396. Epub 2020 Apr 2.

Department of Chemistry, National Taiwan University, Taipei 106, Taiwan.

Herein, ruthenium (Ru) nanoparticles were anchored on carbon nanotubes (Ru/CNTs) functionalized as catalyst cathodes for non-aqueous Li-CO cells. For cycling tests through a low cut-off capacity (100 mA h g), the origin of battery deterioration resulted from the accumulation of LiCO discharging products on catalytic surfaces, identical to the observations in previous studies. However, the Li-CO cells in this work showed a sudden death within several cycles of high cut-off capacity (500 mA h g), and no LiCO residues were investigated on the cathode. In contrast, Li dendrites and passivation materials (LiOH and LiCO) were generated on Li anodes upon cycling at a limited capacity of 500 mA h g, which dominantly contributed to the battery degradation. A Li foil-replacement method was adopted to make the Ru/CNT cathode perform continuous 100 cycles under a cut-off capacity of 500 mA h g. These results indicate that not only LiCO residues blocked on the active sites of the cathode but also Li dendrites and passivation materials produced on the anode caused Li-CO battery deterioration. Moreover, in the present work, a carbon thin film was deposited on Li metal (C/Li) by a sputtering system for suppressing the dendrite formation upon cycling and promoting the defense of the HO attack from the electrolyte disintegration. The Li-CO cell with a Ru/CNT catalyst and a C/Li anode revealed an improved electrochemical stability of 115 cycles at a limited capacity of 500 mA h g. This proto strategy provided a significant research direction focusing on Li anodes for elevating the Li-CO battery durability.
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http://dx.doi.org/10.1039/d0nr00971gDOI Listing
April 2020

Chemical constituents from the roots of Lindera aggregata and their biological activities.

J Nat Med 2020 Mar 7;74(2):441-447. Epub 2020 Jan 7.

The MOE Key Laboratory of Standardization of Chinese Medicines, SATCM Key Laboratory of New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.

Three new benzylisoquinoline alkaloids, (1'S)-12'-hydroxyl-linderegatine (1), (1S)-5'-O-p-hydroxybenzoyl norreticuline (2), (1R, 1'R)-11,11'-biscoclaurine (3), along with 18 known compounds were isolated from the roots of Lindera aggregata (Sims) Kosterm. Their structures were determined on the basis of extensive spectroscopic analysis (IR, UV, HR-ESI-MS, 1D and 2D NMR). The absolute configurations of three new compounds were determined by comparing their experimental and calculated ECD for the first time. Compounds (4) and (9) showed cytotoxic activities against human colon carcinoma cell line (HCT-116), with IC values of 51.4 and 27.1 μM, respectively. Furthermore, compounds (10) and (11) showed inhibitory activities on nitric oxide production induced by lipopolysaccharide in mouse macrophage RAW 264.7 cells, with IC values of 37.8 and 38.7 μM, respectively.
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http://dx.doi.org/10.1007/s11418-019-01385-6DOI Listing
March 2020

Discovery and Characterization of 1-1,2,3-Triazole Derivatives as Novel Prostanoid EP4 Receptor Antagonists for Cancer Immunotherapy.

J Med Chem 2020 01 2;63(2):569-590. Epub 2020 Jan 2.

Drug Discovery Unit, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences , East China Normal University , 500 Dongchuan Road , Shanghai 200241 , China.

The prostanoid EP4 receptor is one of the key receptors associated with inflammatory mediator PGE-elicited immunosuppression in the tumor microenvironment. Blockade of EP4 signaling to enhance immunity-mediated tumor elimination has recently emerged as a promising strategy for cancer immunotherapy. In our efforts to discover novel subtype-selective EP4 antagonists, we designed and synthesized a class of 1-1,2,3-triazole-based ligands that display low nanomolar antagonism activity toward the human EP4 receptor and excellent subtype selectivity. The most promising compound exhibits single-digit nanomolar potency in the EP4 calcium flux and cAMP-response element reporter assays and effectively suppresses the expression of multiple immunosuppression-related genes in macrophage cells. On the basis of its favorable ADMET properties, compound was chosen for further in vivo biological evaluation. Oral administration of compound significantly inhibited tumor growth in the mouse CT26 colon carcinoma model accompanied by enhanced infiltration of cytotoxic T lymphocytes in the tumor tissue.
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http://dx.doi.org/10.1021/acs.jmedchem.9b01269DOI Listing
January 2020

Element Effects on High-Entropy Alloy Vacancy and Heterogeneous Lattice Distortion Subjected to Quasi-equilibrium Heating.

Sci Rep 2019 Oct 15;9(1):14788. Epub 2019 Oct 15.

Department of Materials Science and Engineering, National Chiao Tung University, 1001 University Road, Hsinchu, 30010, Taiwan.

We applied Simmons-Balluffi methods, positron measurements, and neutron diffraction to estimate the vacancy of CoCrFeNi and CoCrFeMnNi high-entropy alloys (HEAs) using Cu as a benchmark. The corresponding formation enthalpies and associated entropies of the HEAs and Cu were calculated. The vacancy-dependent effective free volumes in both CoCrFeNi and CoCrFeMnNi alloys are greater than those in Cu, implying the easier formation of vacancies by lattice structure relaxation of HEAs at elevated temperatures. Spatially resolved synchrotron X-ray measurements revealed different characteristics of CoCrFeNi and CoCrFeMnNi HEAs subjected to quasi-equilibrium conditions at high temperatures. Element-dependent behavior revealed by X-ray fluorescence (XRF) mapping indicates the effect of Mn on the Cantor Alloy.
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http://dx.doi.org/10.1038/s41598-019-51297-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6794270PMC
October 2019

Clinical value of DPOC for detecting and removing residual common bile duct stones (video).

BMC Gastroenterol 2019 Jul 26;19(1):135. Epub 2019 Jul 26.

Department of Gastroenterology, Lanzhou First People's Hospital, No. 1 of Wujiayuan west Street, Qilihe District, Lanzhou City, 730050, China.

Background: This study aims to evaluate the efficacy and safety of detecting and removing residual common bile duct stones (CBDS) using direct peroralcholangioscopy (DPOC) after performing endoscopic retrograde cholangiopancreatography (ERCP) for stone retrieval.

Methods: From January 5, 2017 to December 27, 2017, a total of 164 cases of choledocholithiasis were treated by ERCP for stone retrieval. According to the inclusion and exclusion criteria, the remaining 79 cases (39 males; mean age: 63.3 years old, range: 52-79 years old) were enrolled in the present study. The maximum transverse stone diameter was 6-15 mm (12.7 ± 4.2 mm), as determined by ERCP. Furthermore, there were 57 cases of multiple stones (number of stones: two in 41 cases, three in nine cases, and ≥ 4 in seven cases), 13 cases of post-mechanical lithotripsy, and nine cases of broken stones.

Results: The overall success rate of DPOC was 94.9% (75/79). Furthermore, 18.7%(14/75) of cases were directly inserted, 72%(54/75) of cases required guide wire assistance, and 9.3%(7/75) of cases were successfully inserted with overtube assistance. The average insertion time was 7-17 min (4.9 ± 2.9 min). Residual stones were detected in 19 cases (25.3%), and all of which were < 5 mm in diameter. Moreover, five cases of formed stones were removed by basket and balloon catheter, while the remaining cases were cleaned after irrigation and suction. There were no serious complications.

Conclusion: DPOC is safe and effective for both the detection and removal of residual CBDS after conventional ERCP.
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http://dx.doi.org/10.1186/s12876-019-1045-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6660680PMC
July 2019

Tuning Redox Active Polyoxometalates for Efficient Electron-Coupled Proton-Buffer-Mediated Water Splitting.

Chemistry 2019 Sep 8;25(49):11432-11436. Epub 2019 Aug 8.

State Key Laboratory for Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, iChem (Collaborative Innovation Center of Chemistry for Energy Materials), Xiamen University, Xiamen, Fujian, 361005, P. R. China.

We present strategies to tune the redox properties of polyoxometalate clusters to enhance the electron-coupled proton-buffer-mediated water splitting process, in which the evolution of hydrogen and oxygen can occur in different forms and is separated in time and space. By substituting the heteroatom template in the Keggin-type polyoxometalate cluster, H ZnW O , it is possible to double the number of electrons and protonation in the redox reactions (from two to four). This increase can be achieved with better matching of the energy levels as indicated by the redox potentials, compared to the ones of well-studied H PW O and H SiW O . This means that H ZnW O can act as a high-performance redox mediator in an electrolytic cell for the on-demand generation of hydrogen with a high decoupling efficiency of 95.5 % and an electrochemical energy efficiency of 83.3 %. Furthermore, the H ZnW O cluster also exhibits an excellent cycling behaviour and redox reversibility with almost 100 % H -mediated capacity retention during 200 cycles and a high coulombic efficiency >92 % each cycle at 30 mA cm .
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http://dx.doi.org/10.1002/chem.201903142DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6851869PMC
September 2019

The relative contributions of intra- and inter-specific variation in driving community stoichiometric responses to nitrogen deposition and mowing in a grassland.

Sci Total Environ 2019 May 21;666:887-893. Epub 2019 Feb 21.

Erguna Forest-Steppe Ecotone Research Station, CAS Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100049, China.

Aims: The stoichiometric characteristics of plant communities are important controller for several fundamental ecological processes. The effects of environmental changes on community stoichiometric characteristics are driven by intra- and inter-specific variation. However, the relative importance of both pathways has seldom been empirically examined.

Methods: We quantified the relative contribution of intra- and inter-specific variation to the changes of community nitrogen (N) and phosphorus (P) concentrations after seven-year factorial N addition and mowing treatments in a semi-arid grassland of northern China.

Results: Nitrogen addition significantly increased community N and P concentrations and N:P ratio. Mowing significantly increased community N concentration and N:P. Intra-specific variation contributed more than inter-specific variation to the total variability of all the nutritional and stoichiometric characteristics, with intra-specific variation accounting for 68%, 70%, and 75% of the total variation in community-level N, P, and N:P, respectively. Negative covariations between the contribution of intra- and inter-specific variation occurred for community N and P concentrations. Further, N addition and mowing interacted to affect the impacts of intra- and inter-specific variation on community N concentration and N:P stoichiometry.

Conclusions: Our results highlight different ways of trait selection for N addition and mowing treatments. Interactions between those two factors make it more difficult to accurately predict the responses of plant-mediated biogeochemical cycles under co-occurrence of environmental changes.
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http://dx.doi.org/10.1016/j.scitotenv.2019.02.322DOI Listing
May 2019

Ursolic acid improves diabetic nephropathy via suppression of oxidative stress and inflammation in streptozotocin-induced rats.

Biomed Pharmacother 2018 Sep 19;105:915-921. Epub 2018 Jun 19.

Institution of Pharmacology, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, China. Electronic address:

Inflammation plays a pivotal role in the pathogenesis of diabetic nephropathy (DN). Overexpression of inflammatory chemokine and cytokines is involved in the development of DN. Ursolic acid (UA), a common pentacyclic triterpenoid compound, has been reported to have myriad benefits and medicinal properties. However, its protective effects against renal injury in streptozotocin (STZ)-induced diabetic rats have not been firmly established. In the current report, we investigated whether UA inhibits oxidative stress and inflammation in the kidneys of STZ-induced diabetic rats. Diabetes mellitus (DM) was induced by STZ (40 mg/ kg, i.v.). Animals were randomly divided into control group (normal saline, i.g.), DN group (normal saline, i.g.), DN + UA group (35 mg/kg UA + normal saline, i.g.) and DN + telmisartan group (12 mg/kg telmisartan + normal saline, i.g.). Fasting blood glucose (FBG) levels were monitored at regular intervals. The administration of compounds started at 5 week and lasted for 8 weeks. At the beginning of 13 week, rats were humanely euthanized, KW/BW, BUN, SCr, SOD and MDA were measured. Histopathological changes in renal tissue were observed after hematoxylin-eosin (HE) staining. Furthermore, the expressions of TNF-α, MCP-1 and IL-1β in kidney were determined by immunohistochemistry and western blot. Our results showed that UA significantly lowered the levels of FBG, KW/BW, BUN, SCr and MDA in diabetic rats. Additionally, the SOD activity in UA treated group was higher than that in DN group. Furthermore, renal structural abnormalities and the elevation of TNF-α, MCP-1 and IL-1β expression level were blocked by the administration of UA. In conclusion, our data demonstrate that UA could be well used as a protective agent to counter renal dysfunction - through antioxidant and anti-inflammatory effects.
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http://dx.doi.org/10.1016/j.biopha.2018.06.055DOI Listing
September 2018

Correction to: Huddling remodels gut microbiota to reduce energy requirements in a small mammal species during cold exposure.

Microbiome 2018 07 9;6(1):126. Epub 2018 Jul 9.

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

Following publication of the original article [1], the authors reported an error in the caption of Fig. 4.
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http://dx.doi.org/10.1186/s40168-018-0509-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6044083PMC
July 2018

[Effects of ferulic acid on the expressions of nephrin and podocin in podocytes of diabetic rats].

Zhongguo Ying Yong Sheng Li Xue Za Zhi 2017 Jun;33(6):564-567

Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China.

Objective: To investigate the effects of ferulic acid (FA) on the streptozocin (STZ) -induced kidney injury in diabetic rats and its possible mechanisms.

Methods: Diabetes was induced in male SD rats by an injection of STZ (40 mg/kg,i.v.). After 72 hours, blood glucose levels were detected and blood glucose levels exceeded 16.7 mmol/L were diagnosed as diabetic model rats. Diabetic model rats were randomly divided into model group and FA group, ten animal in each group. Another 10 healthy male SD rats were treated as control group. The rats in FA group were treated with FA (100 mg/kg, i.g.,qd) from the 5th week since the diabetic rats model was successfully established and lasted for 8 weeks. The levels of blood glucose, body weight, organ coefficient of kidney, blood urea nitrogen and creatinine were tested. HE staining was employed to observe the pathological changes of the renal tissue. Immunohistochemistry was employed to determine the protein of nephrin and podocin.

Results: Compared to control group, the levels of blood glucose, organ coefficient of kidney, blood urea nitrogen(BUN) and serum creatinine(sCr) were increased significantly. Renal cells from model group rats showed atrophied and disordered after HE staining and interstitial proliferation were also appeared in renal tissue of the model group. Meantime, the levels of nephrin and podocin protein were obviously decreased. These changes were significantly attenuated in the model group treated with FA.

Conclusions: FA can evidently ameliorate renal damage in rats with diabetic nephropathy induced by STZ, which might be related to increase the level of nephrin and podocin protein.
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http://dx.doi.org/10.12047/j.cjap.5601.2017.133DOI Listing
June 2017

Huddling remodels gut microbiota to reduce energy requirements in a small mammal species during cold exposure.

Microbiome 2018 06 8;6(1):103. Epub 2018 Jun 8.

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

Background: Huddling is highly evolved as a cooperative behavioral strategy for social mammals to maximize their fitness in harsh environments. Huddling behavior can change psychological and physiological responses. The coevolution of mammals with their microbial communities confers fitness benefits to both partners. The gut microbiome is a key regulator of host immune and metabolic functions. We hypothesized that huddling behavior altered energetics and thermoregulation by shaping caecal microbiota in small herbivores. Brandt's voles (Lasiopodomys brandtii) were maintained in a group (huddling) or as individuals (separated) and were exposed to warm (23 ± 1 °C) and cold (4 ± 1 °C) air temperatures (T).

Results: Voles exposed to cold T had higher energy intake, resting metabolic rate (RMR) and nonshivering thermogenesis (NST) than voles exposed to warm T. Huddling voles had lower RMR and NST than separated voles in cold. In addition, huddling voles had a higher surface body temperature (T), but lower core body temperature (T) than separated voles, suggesting a lower set-point of T in huddling voles. Both cold and huddling induced a marked variation in caecal bacterial composition, which was associated with the lower T. Huddling voles had a higher α and β-diversity, abundance of Lachnospiraceae and Veillonellaceae, but lower abundance of Cyanobacteria, Tenericutes, TM7, Comamonadaceae, and Sinobacteraceae than separated voles. Huddling or cold resulted in higher concentrations of short-chain fatty acids (SCFAs), particularly acetic acid and butyric acid when compared to their counterparts. Transplantation of caecal microbiota from cold-separated voles but not from cold-huddling voles induced significant increases in energy intake and RMR compared to that from warm-separated voles.

Conclusions: These findings demonstrate that the remodeling of gut microbiota, which is associated with a reduction in host T, mediates cold- and huddling-induced energy intake and thermoregulation and therefore orchestrates host metabolic and thermal homeostasis. It highlights the coevolutionary mechanism of host huddling and gut microbiota in thermoregulation and energy saving for winter survival in endotherms.
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http://dx.doi.org/10.1186/s40168-018-0473-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5994089PMC
June 2018

Pharmacokinetic Comparisons of Typical Constituents in and Vinegar-Processed after Oral Administration to Rats.

Evid Based Complement Alternat Med 2018 14;2018:6809497. Epub 2018 Mar 14.

School of Pharmacy, Nanjing University of Chinese Medicine, No. 138 Xian Lin Road, Nanjing 210023, China.

The Raw (R-CR), included in the , is a well-known Chinese herbal medicine. However, the vinegar-processed (V-CR) is used more widely than R-CR. The pharmacokinetics comparison of R-CR and V-CR after oral administration to rats is poorly understood. A novel method, rapid resolution liquid chromatography-tandem mass spectrometry (RRLC-MS) coupled with a sensitive, specific, and convenient microdialysis sampling method, free from endogenous interference was developed in this research. The extracts of R-CR and V-CR were administered orally to each group of rats. The blood and liver microdialysis probes were positioned within the jugular vein toward the right atrium and the median lobe near the center of the liver, respectively. Then, a double-peak phenomenon was observed in the concentration-time curves of curdione in R-CR group, while it was not observed in V-CR group. The liver-to-blood distribution ratio of curdione in V-CR group increased significantly ( < 0.05) compared to that of R-CR group. However, compared with V-CR group, the pharmacokinetic parameters of curcumol exhibited no statistically significant differences from those of R-CR group. These results indicate that vinegar-processed procedure has influence on the pharmacokinetic process of in/ns. RRLCMS coupled with microdialysis system could be used to evaluate the pharmacokinetics of typical constituents in after oral administration.
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http://dx.doi.org/10.1155/2018/6809497DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872589PMC
March 2018

Ursolic acid ameliorates oxidative stress, inflammation and fibrosis in diabetic cardiomyopathy rats.

Biomed Pharmacother 2018 Jan 14;97:1461-1467. Epub 2017 Dec 14.

Department of Pharmacology, College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, China. Electronic address:

Diabetic cardiomyopathy is a major and severe cardiovascular complication of diabetes mellitus. Ursolic acid, a pentacyclic triterpene compound widespread in fruits and plants, performs a variety of pharmacological activities including lowering blood glucose, anti-oxidation, anti-inflammation and anti-fibrosis. Our present study aimed to investigate the cardioprotective effects of ursolic acid on diabetic cardiomyopathy rats and uncover its underlying mechanism. Diabetes mellitus was induced by a single injection of STZ-only (40 mg/ kg, i.v.) in male SD rats. Animals were divided into three groups (n=10): control group (normal saline, i.g.), diabetic group (normal saline, i.g.) and diabetic+ursolic acid group (35 mg/kg UA + normal saline, i.g.). Rats were administered for 8 weeks from 5th to 12th week. After the last administration, cardiac function was evaluated; HWI was calculated; FBG, CK, LDH in serum and SOD, MDA in cardiac tissue were detected. HE staining and Masson trichrome staining were employed to observe pathological alterations. Immunohistochemistry and western blotting were taken to determine the expression levels of TNF-α, MCP-1, TGF-β and MMP-2 in the heart. The results dramatically showed increased levels of FBG, CK, LDH, MDA and a decreased activity of SOD in diabetic group, in which left ventricular dysfunction, cardiac myocytes hypertrophy, inflammatory cell infiltration and myocardial interstitial fibrosis had also been found. What's more, the expressions of TNF-α, MCP-1 and TGF-β1 were significantly up-regulated and the expression of MMP-2 was markedly down-regulated in myocardium. Interestingly, treatment with ursolic acid remarkably ameliorated these changes. Collectively, our study strongly showed that ursolic acid is capable of improving the cardiac structure and function in STZ-induced diabetic cardiomyopathy rats by attenuating oxidative stress, inflammation and fibrosis.
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http://dx.doi.org/10.1016/j.biopha.2017.11.032DOI Listing
January 2018

Anatomic isolated caudate lobectomy: Is it possible to establish a standard surgical flow?

World J Gastroenterol 2017 Nov;23(41):7433-7439

Department of General Surgery, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, Zhejiang Province, China.

Aim: To establish the surgical flow for anatomic isolated caudate lobe resection.

Methods: The study was approved by the ethics committee of the Second Affiliated Hospital Zhejiang University School of Medicine (SAHZU). From April 2004 to July 2014, 20 patients were enrolled who underwent anatomic isolated caudate lobectomy at SAHZU. Clinical and postoperative pathological data were analyzed.

Results: Of the total 20 cases, 4 received isolated complete caudate lobectomy (20%) and 16 received isolated partial caudate lobectomy (80%). There were 4 cases with the left approach (4/20, 20%), 6 cases with the right approach (6/20, 30%), 7 cases with the bilateral combined approach (7/20, 35%), 3 cases with the anterior approach (3/20, 15%), and the hanging maneuver was also combined in 2 cases. The median tumor size was 5.5 cm (2-12 cm). The median intra-operative blood loss was 600 mL (200-5700 mL). The median intra-operative blood transfusion volume was 250 mL (0-2400 mL). The median operation time was 255 min (110-510 min). The median post-operative hospital stay was 14 d (7-30 d). The 1- and 3-year survival rates for malignant tumor were 88.9% and 49.4%, respectively.

Conclusion: Caudate lobectomy was a challenging procedure. It was demonstrated that anatomic isolated caudate lobectomy can be done safely and effectively.
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http://dx.doi.org/10.3748/wjg.v23.i41.7433DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5685849PMC
November 2017

Population-based Cohort Study in Outcome of Phased Progression of Atherosclerosis in China (PERSUADE): objective, rationale and design.

J Geriatr Cardiol 2017 Aug;14(8):491-495

Department of Cardiology, Chinese PLA General Hospital, Beijing, China.

The coronary artery calcification score and pericardial fat volume have recently been reported to be strongly associated with the severity and presence of coronary atherosclerosis. However, no studies have explored the outcome of phased progression of atherosclerosis by non-contrast computed tomography in asymptomatic people in China. The population-based cohort study in outcome of phased progression of atherosclerosis in China (PERSUADE), an observational, longitudinal and prospective cohort study in a target population of healthy subjects based in Jidong Oilfield (China), prospectively analyzes the outcome of phased progression of atherosclerosis by non-contrast computed tomography in healthy population. The results of this study are expected to be of value for utilizing noninvasive imaging combine with traditional cardiovascular risk factors to create a risk stratification and find pertinent biomarkers associated with the outcome of phased progression of atherosclerosis in healthy people, thereby could help to establish a more personalized treatment of clinical practice.
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http://dx.doi.org/10.11909/j.issn.1671-5411.2017.08.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5653894PMC
August 2017

The combination of stem cells and tissue engineering: an advanced strategy for blood vessels regeneration and vascular disease treatment.

Stem Cell Res Ther 2017 09 15;8(1):194. Epub 2017 Sep 15.

Department of Cardiovascular Surgery & Institute of Cardiovascular Science, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.

Over the past years, vascular diseases have continued to threaten human health and increase financial burdens worldwide. Transplantation of allogeneic and autologous blood vessels is the most convenient treatment. However, it could not be applied generally due to the scarcity of donors and the patient's condition. Developments in tissue engineering are contributing greatly with regard to this urgent need for blood vessels. Tissue engineering-derived blood vessels are promising alternatives for patients with aortic dissection/aneurysm. The aim of this review is to show the importance of advances in biomaterials development for the treatment of vascular disease. We also provide a comprehensive overview of the current status of tissue reconstruction from stem cells and transplantable cellular scaffold constructs, focusing on the combination of stem cells and tissue engineering for blood vessel regeneration and vascular disease treatment.
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http://dx.doi.org/10.1186/s13287-017-0642-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603030PMC
September 2017

Crystal structure of -(7-di-bromo-methyl-5-methyl-1,8-naphthyridin-2-yl)benzamide-pyrrolidine-2,5-dione (1/1).

Acta Crystallogr E Crystallogr Commun 2017 Jan 1;73(Pt 1):1-3. Epub 2017 Jan 1.

College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650500, People's Republic of China.

The title compound, CHBrNO·CHNO, is a co-crystal of -(7-di-bromo-methyl-5-methyl-1,8-naphthyridin-2-yl)benzamide and pyrrolidine-2,5-dione (succinimide). The benzamide mol-ecule exhibits pseudo-mirror symmetry, with an r.m.s. deviation of the non-H atoms of 0.09 Å (except for the two Br atoms). The angle between the least-squares planes of the two mol-ecules is 26.2 (2)°. In the crystal, the two mol-ecules are mutually linked by N-H⋯O and N-H⋯N hydrogen bonds. The packing is consolidated by C-H⋯(O,N) hydrogen bonds and π-π stacking inter-actions.
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http://dx.doi.org/10.1107/S2056989016019034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5209757PMC
January 2017

Effects of Liraglutide on Reperfusion Injury in Patients With ST-Segment-Elevation Myocardial Infarction.

Circ Cardiovasc Imaging 2016 Dec;9(12)

From the Department of Cardiology (W.R.C., Y.D.C., F.T., S.Y.H., J.W., J.J.Y., S.F.W., X.F.G.) and Division of MRI, Department of Radiology (N.Y., L.Q.C.), PLA General Hospital at Beijing, China.

Background: Liraglutide, a glucagon-like peptide-1 analog, was reported to reduce reperfusion injury in mice. We planned to evaluate the effects of liraglutide on reperfusion injury in patients with acute ST-segment-elevation myocardial infarction treated with primary percutaneous coronary intervention.

Methods And Results: A total of 96 patients with ST-segment-elevation myocardial infarction undergoing emergency primary percutaneous coronary intervention were randomized to receive either subcutaneous liraglutide or placebo. Study treatment was commenced 30 minutes before intervention (1.8 mg) and maintained for 7 days after the procedure (0.6 mg for 2 days, 1.2 mg for 2 days, followed by 1.8 mg for 3 days). The salvage index was calculated from myocardial area at risk, measured during the index admission (35±12 hours), and final infarct size measured at 91±5 days after primary percutaneous coronary intervention by cardiac magnetic resonance. At 3 months, the primary end point, a higher salvage index was found in the liraglutide group than in the placebo group in 77 patients evaluated with cardiac magnetic resonance (0.66±0.14 versus 0.55±0.15; P=0.001). The final infarct size was lower in the liraglutide group than that in the placebo group (15±12 versus 21±15 g; P=0.05). Serum high-sensitivity C-reactive protein level was lower in the liraglutide group (P<0.001). During a 6-month follow-up period, no difference was observed in the incidence of major adverse cardiovascular event. Safety and tolerability were similar among the 2 groups.

Conclusions: Our study provides evidence that liraglutide improves myocardial salvage and infarct size after ST-segment-elevation myocardial infarction, possibly by reducing reperfusion injury, making it a promising treatment for evaluation in larger trials.

Clinical Trial Registration: URL: https://www.clinicaltrials.gov. Unique identifier: NCT02001363.
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http://dx.doi.org/10.1161/CIRCIMAGING.116.005146DOI Listing
December 2016
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