Publications by authors named "Jun-Feng Wang"

147 Publications

miR-124-3p targeted SIRT1 to regulate cell apoptosis, inflammatory response, and oxidative stress in acute myocardial infarction in rats via modulation of the FGF21/CREB/PGC1α pathway.

J Physiol Biochem 2021 Jun 19. Epub 2021 Jun 19.

Department of Neurology, Taihe Hospital, Hubei University of Medicine, No. 32, South Renmin Road, Shiyan City, 442000, Hubei Province, China.

To investigate whether miR-124-3p influences cell apoptosis, inflammatory response, and oxidative stress in rats with acute myocardial infarction (AMI) by mediating the SIRT1/FGF21/CREB/PGC1α pathway. A dual-luciferase reporter gene assay was performed to verify the relationship between miR-124-3p and SIRT1. AMI rats were established via coronary artery ligation after injection with agomiR-124-3p, antagomiR-124-3p, and/or SIRT1 siRNA, and triphenyltetrazolium chloride (TTC), HE, and TUNEL stainings were performed. Bio-Plex rat cytokine assays were performed to determine proinflammatory factor levels. qRT-PCR and Western blotting were used to examine the mRNA and protein expression, respectively. The activity levels of antioxidant enzymes in myocardial tissues were also measured. miR-124-3p was confirmed to target SIRT1 in the H9C2 cells. AMI rats exhibited increased miR-124-3p expression and decreased SIRT1 expression in myocardial tissues. HE staining showed a disorganized cell arrangement and inflammatory cell infiltration in the myocardial tissues of the AMI rats, which was more severe in the rats injected with SIRT1 and agomiR-124-3p but was ameliorated in those treated with antagomiR-124-3p. Moreover, the AMI rats in the antagomiR-124-3p group presented with a reduction in infarct area with an increase in antioxidant enzyme activity, Bcl-2 expression, and activation of the FGF21/CREB/PGC1α pathway, as well as a decrease in cell apoptosis rate, Bax and Caspase-3 expression, and levels of proinflammatory factors, effects that were reversed by si-SIRT1. Inhibiting miR-124-3p expression may activate the FGF21/CREB/PGC1α pathway to reduce cell apoptosis, alleviate the inflammatory response, and attenuate oxidative stress in AMI rats by targeting SIRT1. Graphical abstract.
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http://dx.doi.org/10.1007/s13105-021-00822-zDOI Listing
June 2021

Biomineralization of Cd and inhibition on rhizobacterial Cd mobilization function by Bacillus Cereus to improve safety of maize grains.

Chemosphere 2021 Jun 10;283:131095. Epub 2021 Jun 10.

Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou, 510632, China. Electronic address:

Reducing cadmium (Cd) bioavailability and rhizobacterial Cd mobilization functions in the rhizosphere via the inoculation of screened microbial inoculum is an environmental-friendly strategy to improve safety of crop grains. In this study, Bacillus Cereus, a model Cd resistant strain, was selected to explore its effects on Cd bioavailability and uptake, bacterial metabolic functions related to Cd mobilization. Results indicated that inoculation of Bacillus Cereus in maize roots of sand pot with water-soluble Cd (0.06-0.15 mg/kg) and soil pot with high Cd-contaminated soil (total Cd: 2.33 mg/kg; Cd extracted by NHNO: 38.6 μg/kg) could decrease water-soluble Cd ion concentration by 7.7-30.1% and Cd extracted with NHNO solution by 7.8-22.5%, inducing Cd concentrations in maize grains reduced by 10.6-39.9% and 17.4-38.6%, respectively. Even for a single inoculation in soil, Cd concentration in maize grains still satisfy food safety requirements (Cd content: 0.1 mg/kg dry weight) due to its successful colonization on root surface of maize. Bacillus Cereus could enrich more plant growth promotion bacteria (PGPB) and down-regulate the expression of genes related to bacterial motility, membrane transports, carbon and nitrogen metabolism in the rhizosphere soil, decreasing Cd bioavailability in soil. Approximately 80% Cd in media was transferred into intracellular, meanwhile Cd salts (sulfide and/or phosphate) were produced in Bacillus Cereus through biomineralization process. Overall, this study could provide a feasible method for improving safety of maize grains via the inoculation of Bacillus Cereus under Cd pollution.
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http://dx.doi.org/10.1016/j.chemosphere.2021.131095DOI Listing
June 2021

Nitrogen fertilizer affects rhizosphere Cd re-mobilization by mediating gene AmALM2 and AmALMT7 expression in edible amaranth roots.

J Hazard Mater 2021 Jun 6;418:126310. Epub 2021 Jun 6.

Key Laboratory of Environmental Pollution and Health of Guangdong Province, School of Environment, Jinan University, Guangzhou 510632, China. Electronic address:

In-situ stabilization of Cd-contaminated farmland is a commonly used remediation technology. Yet, rhizosphere metabolites (e.g., organic acids) during crop cultivation may cause Cd re-mobilization and over-accumulation. Here, we identified four pivotal cytomembrane-localized genes underlying Cd accumulation difference between two contrasting edible amaranth cultivars based on root gene expression profile, studied their subcellular localization and functional characteristics, and then investigated effects of nitrogen fertilizer on their expression and rhizosphere Cd re-mobilization. Results showed that more Cd accumulated by edible amaranth was due to rhizosphere Cd mobilization by mediating high expression of AmALMT2 and AmALMT7 genes, not Cd transporters in roots. This was confirmed by heterologous expression of AmALMT2 and AmALMT7 genes in Arabidopsis thaliana, since they mediated malic, fumaric, succinic, and aspartic acids efflux. Furthermore, nitrogen influencing rhizosphere acidification might be closely associated with organic acids efflux genes. Compared with N-NO application, N-NH was massively assimilated into glutamates and oxaloacetates through up-regulating glutamine synthetase and alanine-aspartate-glutamate metabolic pathways, thereby enhancing TCA cycle and organic acids efflux dominated by binary carboxylic acids via up-regulating AmALMT2 and AmALMT7 genes, which finally caused Cd re-mobilization. Therefore, N-NO-dominated nitrogen retarded rhizosphere Cd re-mobilization via inhibiting organic acids efflux function of AmALMT2 and AmALMT7 proteins.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126310DOI Listing
June 2021

Robust coherent control of solid-state spin qubits using anti-Stokes excitation.

Nat Commun 2021 May 28;12(1):3223. Epub 2021 May 28.

CAS Key Laboratory of Quantum Information, University of Science and Technology of China, Hefei, Anhui, People's Republic of China.

Optically addressable solid-state color center spin qubits have become important platforms for quantum information processing, quantum networks and quantum sensing. The readout of color center spin states with optically detected magnetic resonance (ODMR) technology is traditionally based on Stokes excitation, where the energy of the exciting laser is higher than that of the emission photons. Here, we investigate an unconventional approach using anti-Stokes excitation to detect the ODMR signal of silicon vacancy defect spin in silicon carbide, where the exciting laser has lower energy than the emitted photons. Laser power, microwave power and temperature dependence of the anti-Stokes excited ODMR are systematically studied, in which the behavior of ODMR contrast and linewidth is shown to be similar to that of Stokes excitation. However, the ODMR contrast is several times that of the Stokes excitation. Coherent control of silicon vacancy spin under anti-Stokes excitation is then realized at room temperature. The spin coherence properties are the same as those of Stokes excitation, but with a signal contrast that is around three times greater. To illustrate the enhanced spin readout contrast under anti-Stokes excitation, we also provide a theoretical model. The experiments demonstrate that the current anti-Stokes excitation ODMR approach has promising applications in quantum information processing and quantum sensing.
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http://dx.doi.org/10.1038/s41467-021-23471-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8163787PMC
May 2021

Regulation of heavy metals accumulated by Acorus calamus L. in constructed wetland through different nitrogen forms.

Chemosphere 2021 Oct 11;281:130773. Epub 2021 May 11.

School of Environment, Beijing Normal University, Beijing, 100875, China. Electronic address:

Improving accumulation of heavy metals (HMs) by plants is an important pathway for constructed wetland (CW) to alleviate the environmental risks caused by their release. This study aims to regulate HMs (Cr, Ni, Cu, Zn, and Cd) accumulated by Acorus calamus L. in the sandy substrate CW with different nitrogen forms, including ammonia (NH), nitrate (NO‾), and NH/NO‾ (1:1) in synthetic tailwaters. In general, the removal efficiency of HMs by CW could reach 92.4% under the initial concentrations below 500 μg/L. Accumulation percentages of HMs in the shoots and roots of plants in CW with NH and NH/NO‾ influents increased by 52-395% and 15-101%, respectively, when compared with that of NO‾ treatment. Influents with NH promoted plant growth of Acorus calamus L. and metabolic functions, such as carbohydrate metabolism/amino acid metabolism, related to HMs mobilization of rhizosphere bacterial communities, which might induce more organic acids and amino acids secreted by plants and microbes during their metabolic processes. These are the main reasons for the enhancive mobilization of HMs from their precipitation fractions and their uptake by plants in CW with NH treatments. Moreover, the enhancement of organics secreted from plants and microbes also led to the high denitrification efficiency and nitrogen removal in CW. Overall, this study could provide a feasible method for the enhancive accumulation of HMs by wetland plants via the regulation water treatment process to appropriately increase NH for CW.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130773DOI Listing
October 2021

Integration of CW-MFC and anaerobic granular sludge to explore the intensified ammonification-nitrification-denitrification processes for nitrogen removal.

Chemosphere 2021 Sep 1;278:130428. Epub 2021 Apr 1.

State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing, 100875, China. Electronic address:

The integration of constructed wetland-microbial fuel cell (CW-MFC) and anaerobic granular sludge (AGS) is an important way to promote its ammonification efficiency and decrease the land use scale. This study explored the integration of CW-MFC and AGS for nitrogen removal via the intensified ammonification-nitrification-denitrification processes with initial NH-N, NO-N, Org-N and total nitrogen (TN) concentrations of 10.5, 13.8, 21.4, and 45.7 mg L in wastewater. Two reactors with AGS inoculated with a separated area (R1) and directly inoculated into gravel substrate (R2) were designed, respectively. Results showed that chemical oxygen demand (COD) removal efficiency could reach 85% in R1 and 81% in R2, and the conversion of Org-N to NH-N and NO-N to gaseous nitrogen were 80% and 90%, respectively. Although the conversion efficiency of NH-N to NO-N/NO-N via nitrification process was only 18%, it could reach 45%, 94%, and 98% with the aeration rates of 50-, 100-, and 200-mL min. According to microstructural property and microbial community analyses, the separation gravel substrate and AGS areas in R1 availed for stable particle size of AGS, archaeal diversity, and metabolic activity even with a 1.5 times daily wastewater treatment capacity than that of R2. Overall, although the intensified ammonification-nitrification-denitrification processes for nitrogen removal could be achieved with supplementary aeration, further investigation is still needed to explore other substrate materials and high CW-MFC/AGS volume ratio for intensified nitrification process in CW-MFC associated with AGS.
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http://dx.doi.org/10.1016/j.chemosphere.2021.130428DOI Listing
September 2021

[Effects of Precipitation Changes on Plant Community Diversity and Soil C:N:P Ecological Stoichiometric Characteristics in a Desert Steppe of China].

Huan Jing Ke Xue 2021 Feb;42(2):977-987

Gaolan Comprehensive Experimental Station of Ecology and Agriculture, Northwest Institute of Ecology and Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China.

Studying the influence of precipitation patterns on plant community diversity, soil C:N:P ecological stoichiometric characteristics, and the relationships between key soil factors and plant community diversity is of great significance for the protection of plant community diversity in desert grasslands. This paper was studied in the desert steppe of the west of Loess Plateau using a three-year precipitation manipulation experiment (40% reduction in precipitation, 20% reduction in precipitation, natural precipitation, 20% increase in precipitation, and 40% increase in precipitation), explored the influence of changes in precipitation in dry and wet years on the diversity of plant community and soil C:N:P ecological stoichiometric characteristics. And we also explored the relationship between soil C:N:P ecological stoichiometric characteristics and the key soil factors and the diversity of plant community under changes in precipitation. The results showed that in a normal year and the drier year (2013 and 2015), Patrick richness and Shannon-Wiener diversity index were significantly low under the 20% reduction treatment compared with the control and 40% increase treatments, respectively. During the wetter year, Patrick richness and Shannon-Wiener diversity index were no different between any of the precipitation treatments. In the normal year and the drier year, the soil organic carbon (SOC), total nitrogen (TN), and total phosphorus (TP) contents and the carbon-nitrogen ratio (C:N), carbon-phosphorus ratio (C:P), and nitrogen-phosphorus ratio (N:P) all decreased with an increase in precipitation (the decrease in the C:N ratio was statistically significant). During the wetter year, SOC, TN, C:P, and N:P increased with an increase in precipitation. During the normal year, precipitation treatments had no significant influence on soil water content, having a limited influence on the plant community. TN, N:P, SOC, C:N, and microbial biomass nitrogen (MBN) had a more prominent influence on plant community diversity. In the wetter year, precipitation was abundant leading to a rise in soil nutrients. Water was not the most important factor limiting to plant growth whereas soil water content, soil nutrients, and ecological stoichiometric characteristics jointly regulate plant community diversity. In the drier years, precipitation treatments had a significant impact on soil water content, whereby an increase in precipitation led to high losses of soil nutrients. Therefore, soil water content was the most important factor affecting plant community diversity during drier years. These observations indicate that under dry and wet years, plant community diversity and soil C:N:P ecological stoichiometric characteristics have variable responses to precipitation and soil C:N:P effect on plant community were also different. These results provide a theoretical basis for the protection and management of desert steppe systems under future projected changes in precipitation.
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http://dx.doi.org/10.13227/j.hjkx.202007041DOI Listing
February 2021

Pyrrolyl 4-quinolone alkaloids from the mangrove endophytic fungus Penicillium steckii SCSIO 41025: Chiral resolution, configurational assignment, and enzyme inhibitory activities.

Phytochemistry 2021 Jun 16;186:112730. Epub 2021 Mar 16.

CAS Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, PR China; Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning, 530200, PR China; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, 511458, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China. Electronic address:

Six undescribed 4-quinolone alkaloids, including four racemic mixtures, (±)-oxypenicinolines A-D, and two related ones, penicinolines F and G, together with seven known analogues, were isolated from the mangrove-derived fungus Penicillium steckii SCSIO 41025 (Trichocomaceae). The racemates were separated by HPLC using chiral columns. Their structures including absolute configurations were elucidated by extensive spectroscopic analysis, electronic circular dichroism (ECD) experiments, and single-crystal X-ray diffraction analysis. Structurally, (±)-oxypenicinolines A-D shared with an unusual 6/6/5/5 tetracyclic system incorporating a rare tetrahydro-pyrrolyl moiety. A plausible biosynthetic pathway for pyrrolyl 4-quinolone alkaloids is proposed. (±)-oxypenicinoline A and quinolactacide displayed α-glucosidase inhibitory activity with the IC values of 317.8 and 365.9 μΜ, respectively, which were more potent than that of acarbose (461.0 μM). Additionally, penicinoline and penicinoline E showed weak inhibitions toward acetylcholinesterase (AChE).
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http://dx.doi.org/10.1016/j.phytochem.2021.112730DOI Listing
June 2021

Effects of seed size and bract of on germination and seedling growth under drought stress.

Ying Yong Sheng Tai Xue Bao 2021 Feb;32(2):399-405

Ministry of Education Key Laboratory of Vegetation Ecology, Institute of Grassland Science, School of Life Sciences, Northeast Normal University, Changchun 130024, China.

Drought is a crucial factor affecting seed germination and seedling growth of desert plants. In the study, we examined the effects of seed size (large, small) and bract (without bracts, with bracts) on seed germination and seedling growth of , a perennial super xerophyte semi-shrub, under different drought levels (0, 100, 200, 300 and 400 g·L PEG6000). The results showed that drought stress significantly inhibited seed germination and reduced shoot length of seedlings. Under the 100 and 200 g·L PEG6000 stress conditions, root length of seedlings were significantly increased, whereas it was significantly reduced under the 300 and 400 g·L PEG6000 stress intensities. The presence of bract significantly reduced seed germination percentage by 12%, germination index by 50.5%, and shoot length by 20.8%, but increased root length by 6.3%. Seed size did not affect seed germination index. Compared with small seeds, germination percentage of large seeds was 3% higher, shoot length and root length of seedlings were 20.5% and 33.0% higher, respectively. In summary, seed bract in through delaying seed germination and seed size through influencing the survival of the early state of seedling were the most important strategies to adapt extremely arid environment.
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http://dx.doi.org/10.13287/j.1001-9332.202102.001DOI Listing
February 2021

Nitrogen fertilizer management affects remobilization of the immobilized cadmium in soil and its accumulation in crop tissues.

Environ Sci Pollut Res Int 2021 Jun 20;28(24):31640-31652. Epub 2021 Feb 20.

College of Resources and Environment, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China.

Immobilization of soil cadmium (Cd) has been the strategy mostly used in remediation of Cd-contaminated arable soil. However, Cd might be remobilized after the immobilization process through the acid-soluble and complexation effects. Development of agronomic management technologies to prevent soil Cd remobilization after the immobilization process was an important pathway to control the food safety of agricultural products in soils with the immobilized Cd. In this study, the ammonia (NH-N) and nitrate (NO-N) forms with concentrations of 60, 90, and 150 mg-N kg soil were performed for evaluating their effects on Cd remobilization with planted or unplanted treatments and Cd accumulation in tissues of edible amaranth (Liuye). With an initial soil palygorskite-bound fraction Cd concentration of 0.6 mg kg, bioavailable Cd in rhizosphere soils and Cd in crop shoots respectively increased from 11.4 to 20.6 μg kg (dry soil weight) and 6.92 to 14.92 mg kg (dry plant weight) in planted NH-N treatments, while significantly lower concentrations of bioavailable Cd in rhizosphere soils and Cd in crop tissues were observed with planted NO-N treatments. Compared with that of planted NO-N treatments, decreasing pH value (i.e., 7.64 to 7.18) induced by root proton efflux during the absorption of NH-N, enhancive organic/amino acid (oxalic acid, lactic acid, L-proline, and so on) secretion from roots, and increasing abundance of bacteria distributed in phyla Proteobacteria, Cyanobacteria, and Bacteroidetes with Cd mobilization ability in rhizosphere soils were the main reasons found in this study for the higher Cd remobilization in soils and Cd accumulation in crop under NH-N treatments. Moreover, the direct effect of NH-N on remobilization of immobilized Cd by upregulating the expression abundances of genes associated with pyruvate metabolism and amino acids metabolism was more significant than that of NO-N. In summary, the use of NO-N as preferred N fertilizer was more efficient to ensure the food safety of agricultural products than that of NH-N in Cd-contaminated arable soil after immobilization process.
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http://dx.doi.org/10.1007/s11356-021-12868-zDOI Listing
June 2021

Diketopiperazine and enterotoxin analogues from the mangrove derived-soil sp. SCSIO 41400 and their biological evaluation.

Nat Prod Res 2020 Dec 24:1-8. Epub 2020 Dec 24.

CAS Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.

A new diketopiperazine, cyclo-(-8-acetoxyl-Pro--Leu) (), together with eight known compounds (-) including three enterotoxins (-), four diketopiperazines (-) and maltol (), were isolated from the mangrove derived-soil sp. SCSIO 41400. The planar structures of all compounds were determined from analysis of NMR spectra, MS, optical rotation and comparing with literature data. The absolute configuration of compound was assigned by electronic circular dichroism (ECD). The isolated compounds (-) were tested for their acetyl cholinesterase (AChE) and pancreatic lipase (PL) enzyme inhibitory activities. Among them, the new diketopiperazine () displayed preferable PL enzyme inhibitory activity with IC value of 27.3 μg/mL, while compounds , and showed weak PL enzyme inhibitory activity. Further molecular docking simulation exhibited that compound could be well bind with the catalytic pocket of the PL. Besides, compound showed moderate antibacterial activity against with MIC value of 12.5 μg/mL, which was comparable to that of the positive control ampicillin with MIC value of 3.125 μg/mL.
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http://dx.doi.org/10.1080/14786419.2020.1864632DOI Listing
December 2020

New thoughts on the diagnosis and treatment of patients with diabetes mellitus in relation to coronavirus disease.

World J Diabetes 2020 Nov;11(11):481-488

Department of Cardiology, The First Hospital of Jilin University, Changchun 130021, Jilin Province, China.

The coronavirus disease 2019 (COVID-19) outbreak that occurred in late 2019 has posed a huge threat to the health of all humans, especially for individuals who already have diabetes mellitus (DM). DM is one of the most serious diseases that affect human health, with high morbidity and rates of complications. Medical scientists worldwide have been working to control blood sugar levels and the complications associated with sugar level alterations, with an aim to reduce the adverse consequences of acute and chronic complications caused by DM. Patients with DM face great challenges during the pandemic owing to not only changes in the allocation of medical resources but also their abnormal autoimmune status, which reduces their resistance to infections. This increases the difficulty in treatment and the risk of mortality. This review presents, from an epidemiological viewpoint, information on the susceptibility of patients with DM to COVID-19 and the related treatment plans and strategies used in this population.
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http://dx.doi.org/10.4239/wjd.v11.i11.481DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7672793PMC
November 2020

D-serine reduces memory impairment and neuronal damage induced by chronic lead exposure.

Neural Regen Res 2021 May;16(5):836-841

College of Public Health; Laboratory Animal Center, North China University of Science and Technology, Tangshan, Hebei Province, China.

Although exogenous D-serine has been applied as a neural regulatory intervention in many studies, the role played by D-serine in hippocampal injuries caused by lead exposure remains poorly understood. Rat models of chronic lead exposure were established through the administration of 0.05% lead acetate for 8 weeks. Simultaneously, rats were administered 30 or 60 mg/kg D-serine, intraperitoneally, twice a day. Our results showed that D-serine treatment shortened the escape latency from the Morris water maze, increased the number of times that mice crossed the original platform location, and alleviated the pathological damage experienced by hippocampal neurons in response to lead exposure. Although D-serine administration did not increase the expression levels of the N-methyl-D-aspartate receptor subtype 2B (NR2B) in the hippocampi of lead-exposed rats, 60 mg/kg D-serine treatment restored the expression levels of NR2A, which are reduced by lead exposure. These findings suggested that D-serine can alleviate learning and memory impairments induced by lead exposure and that the underlying mechanism is associated with the increased expression of NR2A in the hippocampus. This study was approved by the Animal Ethics Committee of North China University of Science and Technology, China (approval No. LX2018155) on December 21, 2018.
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http://dx.doi.org/10.4103/1673-5374.297086DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178793PMC
May 2021

Assessment of Zeolite, Biochar, and Their Combination for Stabilization of Multimetal-Contaminated Soil.

ACS Omega 2020 Oct 16;5(42):27374-27382. Epub 2020 Oct 16.

Jiangxi Provincial Key Laboratory of Pollution Control for Mining & Metallurgy Environmental Pollution Control, Jiangxi University of Science and Technology, Ganzhou 341000, China.

In this study, the natural zeolite and rice husk biochar were mixed as a combination amendment for metal immobilization in a Cd, Pb, As, and W co-contaminated soil. A 90 day incubation study was conducted to investigate the effects of amendments on toxic metal in soil. Zeolite, biochar, and their combination application increased the soil pH and cation exchange capacity. A combination of amendments decreased the bioavailability of Cd, Pb, As, and W. Besides, the potential drawback of biochar application on As and W release was overcome by the combination agent. Zeolite, biochar, and combination treatment decreased total bioavailability toxicity from 335.5 to 182.9, 250.5, and 143.4, respectively, which means that combination was an optimum amendment for soil remediation. The results of the Community Bureau of Reference sequential extraction and scanning electron microscopy-energy-dispersive spectrometry images confirmed the Cd and Pb adsorption onto biochar. However, As and W immobilization was dominantly controlled by zeolite. It appears that the combination of amendments is an efficient amendment to remediate Cd, Pb, As, and W co-contamination in soil, although the combination of amendments has a lower stabilization rate for W than for zeolite.
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http://dx.doi.org/10.1021/acsomega.0c03710DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7594124PMC
October 2020

Combination of D-dimer and simplified pulmonary embolism severity index to improve prediction of hospital death in patients with acute pulmonary embolism.

J Int Med Res 2020 Oct;48(10):300060520962291

Department of Cardiology, The First Hospital of Jilin University, Changchun, China.

Objective: To investigate whether the combination of D-dimer and simplified pulmonary embolism severity index (sPESI) could improve prediction of in-hospital death from pulmonary embolism (PE).

Methods: Patients with PE (n = 272) were divided into a surviving group (n = 249) and an in-hospital death group (n = 23).

Results: Compared with surviving patients, patients who died in hospital had significantly higher rates of hypotension and tachycardia, reduced SaO levels, elevated D-dimer and troponin T levels, higher sPESI scores, and were more likely to be classified as high risk. Elevated D-dimer levels and high sPESI scores were significantly associated with in-hospital death. Using thresholds for D-dimer and sPESI of 3.175 ng/mL and 1.5, respectively, the specificity for prediction of in-hospital death was 0.357 and 0.414, respectively, and the area under the receiver operating characteristic curve (AUC) was 0.665 and 0.668, respectively. When D-dimer and sPESI were considered together, the specificity for prediction of in-hospital death increased to 0.838 and the AUC increased to 0.74.

Conclusions: D-dimer and sPESI were associated with in-hospital death from PE. Considering D-dimer levels together with sPESI can significantly improve the specificity of predicting in-hospital death for patients with PE.
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http://dx.doi.org/10.1177/0300060520962291DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7570299PMC
October 2020

Single-Cell Characterization of Malignant Phenotypes and Developmental Trajectories of Adrenal Neuroblastoma.

Cancer Cell 2020 11 17;38(5):716-733.e6. Epub 2020 Sep 17.

State Key Laboratory of Oncogenes and Related Genes, Renji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China. Electronic address:

Neuroblastoma (NB), which is a subtype of neural-crest-derived malignancy, is the most common extracranial solid tumor occurring in childhood. Despite extensive research, the underlying developmental origin of NB remains unclear. Using single-cell RNA sequencing, we generate transcriptomes of adrenal NB from 160,910 cells of 16 patients and transcriptomes of putative developmental cells of origin of NB from 12,103 cells of early human embryos and fetal adrenal glands at relatively late development stages. We find that most adrenal NB tumor cells transcriptionally mirror noradrenergic chromaffin cells. Malignant states also recapitulate the proliferation/differentiation status of chromaffin cells in the process of normal development. Our findings provide insight into developmental trajectories and cellular states underlying human initiation and progression of NB.
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http://dx.doi.org/10.1016/j.ccell.2020.08.014DOI Listing
November 2020

Physicians' very brief (30-sec) intervention for smoking cessation on 13 671 smokers in China: a pragmatic randomized controlled trial.

Addiction 2021 05 5;116(5):1172-1185. Epub 2020 Oct 5.

School of Public Health, The University of Hong Kong, Hong Kong, China.

Background And Aims: Three to 10 minutes of smoking cessation advice by physicians is effective to increase quit rates, but is not routinely practised. We examined the effectiveness of physicians' very brief (approximately 30 sec) smoking cessation intervention on quit rates among Chinese outpatient smokers.

Design: A pragmatic, open-label, individually randomized controlled trial.

Setting: Seventy-two medical outpatient departments of hospitals and/or community health centers in Guangdong, China.

Participants: Chinese adults who were daily cigarette smokers (n = 13 671, 99% males) were invited by their physician to participate during outpatient consultation. Smokers who were receiving smoking cessation treatment or were judged to need specialist treatment for cessation were excluded.

Interventions: The intervention group (n = 7015) received a 30-sec intervention including physician's very brief advice, a leaflet with graphic warnings and a card with contact information of available cessation services. The control group (n = 6656) received a very brief intervention on consuming vegetables and fruit. A total of 3466 participants in the intervention group were further randomized to receive a brief booster advice from trained study personnel via telephone 1 month following their doctor visit.

Measurements: The primary outcome was self-reported 7-day point prevalence abstinence (PPA) in the intervention and control groups at the 12-month follow-up. Secondary outcomes included self-reported 30-day abstinence and biochemically validated abstinence at 12-month follow-up.

Findings: By intention-to-treat, the intervention (versus control) group had greater self-reported 7-day abstinence [9.1 versus 7.8%, odds ratio (OR) = 1.14, 95% confidence interval (CI) = 1.03-1.26, P = 0.008] and 30-day abstinence (8.0 versus 6.9%, OR = 1.14, 95% CI = 1.03-1.27, P = 0.01) at 12-month follow-up. The effect size increased when only participants who received the intervention from compliant physicians were included (7-day PPA, OR = 1.42, 95% CI = 1.11-1.74). The group difference in biochemically validated abstinence was small (0.8 versus 0.8%, OR = 1.00, 95% CI = 0.71-1.42, P = 0.99).

Conclusion: A 30-sec smoking cessation intervention increased self-reported abstinence among mainly male smokers in China at 12-month follow-up (risk difference = 1.3%), and should be feasible to provide in most settings and delivered by all health-care professionals.
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http://dx.doi.org/10.1111/add.15262DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8246886PMC
May 2021

Role of Barrett's Esophagus and Lymph Node Harvest in Recurrence of Early Esophageal Adenocarcinoma.

Ann Thorac Surg 2021 02 3;111(2):732. Epub 2020 Jul 3.

Department of Thoracic Surgery, Qujing First People's Hospital, No. 1, Yuanlin Alley, Qujing, 655000, China. Electronic address:

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http://dx.doi.org/10.1016/j.athoracsur.2020.05.073DOI Listing
February 2021

MiR-590 Suppresses Proliferation and Induces Apoptosis in Pancreatic Cancer by Targeting High Mobility Group A2.

Technol Cancer Res Treat 2020 Jan-Dec;19:1533033820928143

Department of general surgery, Wuhu Hospital of Traditional Chinese Medicine, Wuhu, Anhui, People's Republic of China.

Background: Pancreatic ductal adenocarcinoma is a common malignancy with high morbidity. MicroRNAs have been demonstrated to be critical posttranscriptional regulators in tumorigenesis. This study aimed to investigate the effect of microRNA-590 on the proliferation and apoptosis of pancreatic ductal adenocarcinoma.

Material And Methods: The expression of microRNA-590 and high mobility group AT-hook 2 were examined in clinical pancreatic ductal adenocarcinoma tissues. Pancreatic ductal adenocarcinoma cell line Capan-2 was employed and transfected with microRNA-590 mimics or inhibitor. The correlation between microRNA-590 and high mobility group AT-hook 2 was verified by luciferase reporter assay. Cell viability and apoptosis were detected by MTT and flow cytometry assay. The protein level of high mobility group AT-hook 2, AKT, p-AKT, mTOR, and phosphorylated mTOR were analyzed by Western blotting.

Results: MicroRNA-590 was found to be negatively correlated with the expression of high mobility group AT-hook 2 in pancreatic ductal adenocarcinoma tissues. Further studies identified high mobility group AT-hook 2 as a direct target of microRNA-590. Moreover, overexpression of microRNA-590 downregulated expression of high mobility group AT-hook 2, reduced cell viability, and promoted cell apoptosis, while knockdown of miR-590 led to an inverse result. MicroRNA-590 also suppressed the phosphorylation of AKT and mTOR without altering total AKT and mTOR levels.

Conclusion: Our study indicated that microRNA-590 negatively regulates the expression of high mobility group AT-hook 2 in clinical specimens and . MicroRNA-590 can inhibit cell proliferation and induce cell apoptosis in pancreatic ductal adenocarcinoma cells. This regulatory effect of microRNA-590 may be associated with AKT signaling pathway. Therefore, microRNA-590 has the potential to be used as a biomarker for predicting the progression of pancreatic ductal adenocarcinoma.
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http://dx.doi.org/10.1177/1533033820928143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7325540PMC
January 2021

Coherent Control of Nitrogen-Vacancy Center Spins in Silicon Carbide at Room Temperature.

Phys Rev Lett 2020 Jun;124(22):223601

CAS Key Laboratory of Quantum Information, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.

Solid-state color centers with manipulatable spin qubits and telecom-ranged fluorescence are ideal platforms for quantum communications and distributed quantum computations. In this work, we coherently control the nitrogen-vacancy (NV) center spins in silicon carbide at room temperature, in which telecom-wavelength emission is detected. We increase the NV concentration sixfold through optimization of implantation conditions. Hence, coherent control of NV center spins is achieved at room temperature, and the coherence time T_{2} can be reached to around 17.1  μs. Furthermore, an investigation of fluorescence properties of single NV centers shows that they are room-temperature photostable single-photon sources at telecom range. Taking advantage of technologically mature materials, the experiment demonstrates that the NV centers in silicon carbide are promising platforms for large-scale integrated quantum photonics and long-distance quantum networks.
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http://dx.doi.org/10.1103/PhysRevLett.124.223601DOI Listing
June 2020

Activin B signaling may promote the conversion of normal fibroblasts to scar fibroblasts.

Medicine (Baltimore) 2020 Jun;99(24):e20253

Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.

This study is to explore the molecular mechanism of benign bile duct hypertrophic scar formation.Differential proteins between the normal fibroblast (NFB) and scar fibroblast (SCFB) were screened by protein chip assay, and analyzed by pathway-enrichment analysis and function-enrichment analysis. The differential proteins were further tested by ELISA. SiRNA-Act B was transfected to SCFB to down-regulate the expression of Act B. NFB was incubated with rh-Act B. The cell apoptosis and cell cycle were determined by flow cytometry. The expression of Act B, Smad2/3, transforming growth factor-β1 (TGF-β1), endothelin-1 (ET-1), thrombospondin-1 (Tsp-1), and Oncostatin M (OSM) were detected by Western blot.A total of 37 differential proteins were identified in SCFBs by microarray (P < .05), including 27 up-regulated proteins and 10 down-regulated proteins (P < .05). Their function were associated with Activin signaling, synthesis and degradation of extracellular matrix, formation and activation of cytokine, inflammatory reaction, immunoreaction, tissue damage reaction, cell cycle, migration, apoptosis, and secretion, etc. ELISA results showed that the expression of Act B, TGF-β1, ET-1 were higher in SCFBs, while the expression of Tsp-1 and OSM were lower in SCFBs (P < .05). After interfered by siRNA-Act B, the expression of Act B mRNA decreased (P < .05). The percentage of early apoptosis increased (P < .05). The expression of Act B, Smad2/3, TGF-β1 were decreased and Tsp-1, OSM were increased (P < .05). After treatment with rh-Act B, the percentage of G0/G1 phase of NFBs was decreased and that of S phase was increased without significance (P > .05). The expression of Act B, Smad2/3, TGF-β1 were increased (P < .05) and Tsp-1, OSM were decreased (P < .01).There are differentially expressed proteins between SCFBs and NFBs. Activin B signal plays an important role in the process of NFB transforming to SCFB, and TGF-β1, Smad2/3, Tsp-1, and OSM are important participants.
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http://dx.doi.org/10.1097/MD.0000000000020253DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7302586PMC
June 2020

[Responses of plant growth of different life-forms to precipitation changes in desert steppe].

Ying Yong Sheng Tai Xue Bao 2020 Mar;31(3):778-786

Gaolan Ecological and Agricultural Integrated Experiment Station, Northwest Institute of Eco-Environment and Resources, Lanzhou 730000, China.

Under the background of global climate change, precipitation changes will have profound impacts on plant community dynamics. Through field experiment with precipitation manipulation in a desert steppe of western Loess Plateau, we examined the responses of species richness, density, coverage, height and aboveground biomass of different plant life-forms to precipitation changes. The results showed significant effects of precipitation on richness, density and coverage of annual herbs in the third year of manipulation experiment (2015), with lowest values in the decreased precipitation treatments. The height of annual herbs was more sensitive to precipitation changes, and was lowest in the -40% precipitation treatment during three years. The magnitudes of negative response of growth and aboveground biomass of annual herbs to decreased precipitation were larger than that to increased precipitation. Richness, density and coverage of perennial herbs in the decreased precipitation were significantly lower than those in the +40% precipitation in the 3rd year, but were insignificantly different from the control. The height of perennial herbs was lowest in the -40% precipitation treatment during three years. The magnitudes of negative response of richness, coverage and height of perennial herbs to decreased precipitation were larger than positive response to increased precipitation, while the positive response of aboveground biomass to the +40% precipitation treatment was stronger. The positive responses of richness, density, coverage and aboveground biomass of shrub to ±20% precipitation treatments were most obvious, which might be related to the relatively concentrated distribution of shrub in these treatments. The precipitation reduction inhibited the growth of herbaceous plants, particularly on the annual herbs, whereas increasing precipitation promoted perennial herbaceous growth and biomass accumulation to some extent. The annual herbaceous growth and biomass fluctuated with interannual variation of precipitation. Shrubs were relatively less affected by precipitation changes. Precipitation changes would have significant effects on plant community composition and function of desert steppe in western Loess Plateau.
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http://dx.doi.org/10.13287/j.1001-9332.202003.001DOI Listing
March 2020

Four new steroids from the marine soft coral-derived fungus Penicillium sp. SCSIO41201.

Chin J Nat Med 2020 Apr;18(4):250-255

CAS Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; University of Chinese Academy of Sciences, Beijing 100049, China; South China Branch of Innovation Academy for Drug Discovery and Development, Chinese Academy of Sciences, Guangzhou 510650, China. Electronic address:

Penicildiones A-D (1-4), four new steroids derivatives together with three known compounds including 16α-methylpregna-17α,19-dihydroxy-(9,11)-epoxy-4-ene-3,18-dione-20-acetoxy (5), stachybotrylactone B (6) and stachybotrin (7) were isolated from the soft coral-derived fungus Penicillium sp. SCSIO41201, cultured in the 1% NaCl PDB substrate. Their structures were determined through spectroscopic methods and X-ray crystallography. Biological evaluation results revealed that 6 exhibited significant cytotoxic activity against HL-60, K562, MOLT-4, ACHN, 786-O, and OS-RC-2 cell lines with IC values of 5.23, 4.12, 4.31, 23.55, 7.65 and 10.81 μmol·L, respectively, while other compounds showed weak or no cytotoxicity at 50 μmol·L.
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http://dx.doi.org/10.1016/S1875-5364(20)30030-3DOI Listing
April 2020

Heterogeneous nuclear ribonucleoprotein A3 controls mitotic progression of neural progenitors via interaction with cohesin.

Development 2020 05 15;147(10). Epub 2020 May 15.

School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China

Cortex development is controlled by temporal patterning of neural progenitor (NP) competence with sequential generation of deep and superficial layer neurons, but underlying mechanisms remain elusive. Here, we report a role for heterogeneous nuclear ribonucleoprotein A3 (HNRNPA3) in regulating the division of early cortical NPs that mainly give rise to deep-layer neurons via direct neurogenesis. HNRNPA3 is expressed at high levels in NPs of mouse and human cortex at early stages, with a unique peri-chromosome pattern. Intriguingly, downregulation of HNRNPA3 caused chromosome disarrangement, which hindered normal separation of chromosomes during NP division, leading to mitotic delay. Furthermore, HNRNPA3 is associated with the cohesin-core subunit SMC1A and controls its association with chromosomes, implicating a mechanism for the role of HNRNPA3 in regulating chromosome segregation in dividing NPs. -deficient mice exhibited reduced cortical thickness, especially of deep layers. Moreover, downregulation of HNRNPA3 in cultured human cerebral organoids led to marked reduction in NPs and deep-layer neurons. Thus, this study has identified a crucial role for HNRNPA3 in NP division and highlighted the relationship between mitosis progression and early neurogenesis.
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http://dx.doi.org/10.1242/dev.185132DOI Listing
May 2020

Antioxidant CPA-type indole alkaloids produced from the deep-sea derived fungus sp. SCSIO 41024.

Nat Prod Res 2020 Apr 7:1-5. Epub 2020 Apr 7.

CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, P.R. China.

Twelve indole alkaloids, including α-cyclopiazonic acid (CPA) (), nine 2-oxo indole CPA derivatives (-), and two open-ring indole CPA derivatives ( and ), were isolated from the fermentation broth of a deep-sea derived fungus sp. SCSIO 41024. Their structures and absolute configurations were elucidated mainly by using extensive NMR spectroscopic, mass spectrometric and single crystal X-ray diffraction analysis. To the best of our knowledge, the crystallographic data of and were firstly reported, and the absolute configuration of was confirmed for the first time by the single crystal X-ray diffraction analysis. Most isolated compounds were tested for their antimicrobial, antitumor and radical scavenging activities. In addition, compounds , and showed moderate antioxidative activity against DPPH with IC values of 190.1, 31.9, 228.4 μg/mL, respectively.
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http://dx.doi.org/10.1080/14786419.2020.1749614DOI Listing
April 2020

Biological mechanisms of cadmium accumulation in edible Amaranth (Amaranthus mangostanus L.) cultivars promoted by salinity: A transcriptome analysis.

Environ Pollut 2020 Jul 4;262:114304. Epub 2020 Mar 4.

Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou, 510632, China; Research Center of Low Carbon Economy for Guangzhou Region, Jinan University, Guangzhou, 510632, China.

Strategies to prevent cadmium (Cd) mobilization by crops under salinity conditions differs among distinct genotypes, but the biological mechanisms of Cd accumulation in different genotype crops promoted by salinity have remained scarce. In this study, we investigated the biological mechanisms of Cd accumulation in two quite different amaranth cultivars of low-Cd accumulator Quanhong (QH) and high-Cd accumulator Liuye (LY) in response to salt stress. Transcriptomes analysis was carried out on leaves and roots tissues of LY and QH grown with exchangeable Cd 0.27 mg kg and salinity 3.0 g kg treatment or control conditions, respectively. A total of 3224 differentially expressed genes (DEGs) in LY (1119 in roots, 2105 in leaves) and 848 in QH (207 in roots, 641 in leaves) were identified. Almost in each fold change category (2-2, 2-2, >2), the numbers of DEGs induced by salinity in LY treatments were much more than those in QH treatments, indicating that LY is more salt sensitive. Gene ontology (GO) analysis revealed that salinity stress promoted soil acidification and Cd mobilization in LY treatments through the enhancive expression of genes related to adenine metabolism (84-fold enrichment) and proton pumping ATPase (50-fold enrichment) in roots, and carbohydrate hydrolysis (2.5-fold enrichment) in leaves compared with that of whole genome, respectively. The genes expression of organic acid transporter (ALMT) was promoted by 2.71- to 3.94-fold in roots, facilitating the secretion of organic acids. Salt stress also inhibited the expression of key enzymes related to cell wall biosynthesis in roots, reducing the physical barriers for Cd uptake. All these processes altered in LY were more substantially compared with that of QH, suggesting that salt sensitive cultivars might accumulate more Cd and pose a higher health risk.
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http://dx.doi.org/10.1016/j.envpol.2020.114304DOI Listing
July 2020

The association of coagulation indicators with in-hospital mortality and 1-year mortality of patients with sepsis at ICU admissions: A retrospective cohort study.

Clin Chim Acta 2020 May 7;504:109-118. Epub 2020 Feb 7.

Department of Intensive Care Unit, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. Electronic address:

Background: Coagulation activation is the host's response to pathogens during sepsis and is considered to be one of the reasons for tissue damage and multiple organ failure. This study is designed to evaluate whether the alterations of coagulation indicators are related to in-hospital mortality and 1-year mortality of patients with sepsis.

Method: Data of all 2258 patients were extracted from the database Multiparameter Intelligent Monitoring in Intensive Care III (MIMIC III). The relationship between the in-hospital mortality of patients with sepsis and coagulation indicators was analyzed with a receiver operating characteristic (ROC) curve analysis and logistic regression model. Effects of coagulation indicators on patients' 1-year mortality were determined by using a Cox hazard regression model, and clinical experience or quintiles were used to classify the activated partial thromboplastin time (APTT) to determine the cutoff values to explore segmentation effects.

Result: International normalized ratio (INR) was positively associated with hospital mortality of patients with sepsis after adjusting confounders with an odds ratio (OR) of 1.86 [95% confidence interval (CI), 1.37-2.52], and a hazard ratio (HR) of 1.465[95%CI(1.24-1.74)] for 1-year mortality, respectively. 1-year mortality of patients with sepsis demonstrated a U-shaped relationship with APTT, ranging from 25 to 37, indicating the lowest risk. The adjusted HR (95% CI) values for 1-year mortality of septic patients with risk values <25 and >37 were 1.493 (1.02, 2.19) and 1.379 (1.06, 1.79), respectively.

Conclusion: Increased INR in critically ill septic patients is related to greater in-hospital mortality and 1-year mortality. A U-shaped relationship was found between APTT and 1-year mortality of patients with sepsis.
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http://dx.doi.org/10.1016/j.cca.2020.02.007DOI Listing
May 2020

The marine-derived furanone reduces intracellular lipid accumulation in vitro by targeting LXRα and PPARα.

J Cell Mol Med 2020 03 24;24(6):3384-3398. Epub 2020 Jan 24.

Institute of Lipid Metabolism and Atherosclerosis, School of Pharmacy, Innovative Drug Research Centre, Weifang Medical University, Weifang, China.

Recent studies have demonstrated that commercially available lipid-lowering drugs cause various side effects; therefore, searching for anti-hyperlipidaemic compounds with lower toxicity is a research hotspot. This study was designed to investigate whether the marine-derived compound, 5-hydroxy-3-methoxy-5-methyl-4-butylfuran-2(5H)-one, has an anti-hyperlipidaemic activity, and the potential underlying mechanism in vitro. Results showed that the furanone had weaker cytotoxicity compared to positive control drugs. In RAW 264.7 cells, the furanone significantly lowered ox-LDL-induced lipid accumulation (~50%), and its triglyceride (TG)-lowering effect was greater than that of liver X receptor (LXR) agonist T0901317. In addition, it significantly elevated the protein levels of peroxisome proliferator-activated receptors (PPARα) and ATP-binding cassette (ABC) transporters, which could be partially inhibited by LXR antagonists, GSK2033 and SR9243. In HepG2 cells, it significantly decreased oleic acid-induced lipid accumulation, enhanced the protein levels of low-density lipoprotein receptor (LDLR), ABCG5, ABCG8 and PPARα, and reduced the expression of sterol regulatory element-binding protein 2 (~32%). PPARα antagonists, GW6471 and MK886, could significantly inhibit the furanone-induced lipid-lowering effect. Furthermore, the furanone showed a significantly lower activity on the activation of the expression of lipogenic genes compared to T0901317. Taken together, the furanone exhibited a weak cytotoxicity but had powerful TC- and TG-lowering effects most likely through targeting LXRα and PPARα, respectively. These findings indicate that the furanone has a potential application for the treatment of dyslipidaemia.
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http://dx.doi.org/10.1111/jcmm.15012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131916PMC
March 2020

Improving cadmium mobilization by phosphate-solubilizing bacteria via regulating organic acids metabolism with potassium.

Chemosphere 2020 Apr 25;244:125475. Epub 2019 Nov 25.

Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou, 510632, China. Electronic address:

Organic acids secreted by phosphorus-solubilizing bacteria (PSB) is one of the main biological metabolites with cadmium (Cd) mobilization capacity in the conversion of insoluble precipitate forms to bioavailable forms in contaminated soil. However, the fluctuating concentrations of nutrient elements caused by agricultural activities may result in the substantial variances of carbohydrate metabolism of microorganisms involved in Cd remediation, it is therefore essential to study how metabolic strategies, especially for organic acids, affected by the environmentally friendly fertilizers, such as potassium (K). In this study, adding K (KCl) concentrations from 0.0 to 100.0 mg/L in medium clearly accelerated Cd mobilization from 15.9 to 35.9 mg/L via inducing the secretion of tartaric acid, 3-hydroxybutyrate, fumaric and succinic acids, increased by 10.0-, 7.5-, 4.3- and 4.1-fold changes, respectively. Current data revealed that the significant differences of metabolic pathways and genes expressions with the varied K concentrations included: ⅰ) K induces a substantial up-regulation in metabolic pathway of pyruvic acid to oxaloacetate and tartaric acids; ⅱ) the varied expression of genes involved in encoding enzymes of tricarboxylic acid cycle result in the up-regulated fumaric acid, succinic acid and 3-hydroxybutyrate; ⅲ) the expression of genes related enzyme cysteine and glutamate metabolism processes promoted with the increasing bioavailable Cd concentrations. Besides, P-type ATPase activity increased with K levels, indicating that H efflux and medium acidification were strengthened. In general, an appropriate enhancement of K based fertilizer is an effective manner for soil Cd remediation via the regulation of organic acids metabolism and H secretion of PSB.
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http://dx.doi.org/10.1016/j.chemosphere.2019.125475DOI Listing
April 2020

Long non-coding RNA RP11-468E2.5 curtails colorectal cancer cell proliferation and stimulates apoptosis via the JAK/STAT signaling pathway by targeting STAT5 and STAT6.

J Exp Clin Cancer Res 2019 Nov 12;38(1):465. Epub 2019 Nov 12.

Department of Abdominal Radiotherapy, Harbin Medical University Tumour Hospital, No. 150, Haping Road, Nangang District, Harbin, 150081, People's Republic of China.

Background: Long non-coding RNAs (lncRNAs) are tumor-associated biological molecules and have been found to be implicated in the progression of colorectal cancer (CRC). This study aims to examine the effects of lncRNA RP11-468E2.5 and its target genes (STAT5 and STAT6) on the biological activities of CRC cells via the Janus kinase-signal transducer and activator of transcription (JAK/STAT) signaling pathway.

Methods: We initially screened the GEO database for differentially expressed lncRNAs related to CRC and then made a prediction of the implicated target genes. Then we collected CRC tissues and adjacent normal tissues from 169 CRC patients. Human CRC HCT116 and SW480 cells were treated with small interference RNA (siRNA) against RP11-468E2.5, AG490 (an inhibitor of the JAK/STAT signaling pathway), or both in combination. Next, we measured the effects of RP11-468E2.5 treatment on cellular activities such as cell viability, cycle distribution and cell apoptosis, and studied interactions among RP11-468E2.5, STAT5/STAT6, and the JAK/STAT signaling pathway. Finally, an in vivo tumor formation assay was performed to observe the effect of RP11-468E2.5 on tumor growth.

Results: The CRC-related gene microarray data showed low expression of RP11-468E2.5 in CRC surgical specimens. However, RP11-468E2.5 was confirmed to target STAT5 and STAT6, which participate in the JAK/STAT signaling pathway. CRC tissues showed lower expression of RP11-468E2.5, higher expression of STAT5, STAT6 and of the cell cycle marker Cyclin D1 (CCND1), compared to the findings in adjacent normal tissues. The treatment of siRNA against RP11-468E2.5 increased expression of JAK2, STAT3, STAT5, STAT6, CCND1 and Bcl-2 along with the extent of STAT3, STAT5 and STAT6 phosphorylation, while lowering expression of P21 and P27. Treatment with AG490 exhibited approximately opposite effects, whereas siRNA against RP11-468E2.5 treatment stimulated CRC cell proliferation and reduced cell apoptosis, while promoting cell cycle entry; AG490 treatment reversed these results.

Conclusions: Altogether, we conclude that up-regulation of RP11-468E2.5 inhibits the JAK/STAT signaling pathway by targeting STAT5 and STAT6, thereby suppressing cell proliferation and promoting cell apoptosis in CRC.
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http://dx.doi.org/10.1186/s13046-019-1428-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6852742PMC
November 2019
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