Publications by authors named "Jun Zhou"

2,915 Publications

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SMDS-Net: Model Guided Spectral-Spatial Network for Hyperspectral Image Denoising.

IEEE Trans Image Process 2022 Aug 11;PP. Epub 2022 Aug 11.

Deep learning (DL) based hyperspectral images (HSIs) denoising approaches directly learn the nonlinear mapping between noisy and clean HSI pairs. They usually do not consider the physical characteristics of HSIs. This drawback makes the models lack interpretability that is key to understanding their denoising mechanism and limits their denoising ability. In this paper, we introduce a novel model-guided interpretable network for HSI denoising to tackle this problem. Fully considering the spatial redundancy, spectral low-rankness, and spectral-spatial correlations of HSIs, we first establish a subspace-based multidimensional sparse (SMDS) model under the umbrella of tensor notation. After that, the model is unfolded into an end-to-end network named SMDS-Net, whose fundamental modules are seamlessly connected with the denoising procedure and optimization of the SMDS model. This makes SMDS-Net convey clear physical meanings, i.e., learning the low-rankness and sparsity of HSIs. Finally, all key variables are obtained by discriminative training. Extensive experiments and comprehensive analysis on synthetic and real-world HSIs confirm the strong denoising ability, strong learning capability, promising generalization ability, and high interpretability of SMDS-Net against the state-of-the-art HSI denoising methods. The source code and data of this article will be made publicly available at https://github.com/bearshng/smds-net for reproducible research.
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http://dx.doi.org/10.1109/TIP.2022.3196826DOI Listing
August 2022

Dexmedetomidine Ameliorated Cognitive Dysfunction Induced by Intestinal Ischemia Reperfusion in Mice with Possible Relation to the Anti-inflammatory Effect Through the Locus Coeruleus Norepinephrine System.

Neurochem Res 2022 Aug 9. Epub 2022 Aug 9.

Department of Anesthesiology, West China Hospital of Sichuan University, Chengdu, China.

Cognitive impairment is a common central nervous system complication that occurs following surgery or organs damage outside the nervous system. Neuroinflammation plays a key role in the molecular mechanisms of cognitive impairment. Dexmedetomidine alleviates neuroinflammation and reduces cognitive dysfunction incidence; however, the mechanism by which dexmedetomidine alleviates cognitive dysfunction remains unclear. This study evaluated the effect of dexmedetomidine on attenuation of early cognitive impairment induced by intestinal ischemia-reperfusion in mice and examined whether the locus coeruleus norepinephrine (LCNE) system participates in the anti-inflammatory effect of dexmedetomidine. The superior mesenteric artery was clamped for 45 min to induce intestinal ischemia reperfusion injury. Dexmedetomidine alone or combined with DSP-4, a selective locus coeruleus noradrenergic neurotoxin, was used for pretreatment. Postoperative cognition was assessed using the Morris water maze. Serum and hippocampal levels of IL-1β, TNF-α, norepinephrine (NE), and malondialdehyde (MDA) were assessed by enzyme-linked immunosorbent assay. Immunofluorescence, immunohistochemistry, and hematoxylin and eosin staining were used to evaluate the expression of tyrosine hydroxylase (TH) in the locus coeruleus, hippocampal microglia, and intestinal injury. Pretreatment with dexmedetomidine alleviated intestinal injury and decreased the serum and hippocampal levels of NE, IL-1β, TNF-α, and MDA at 24 h after intestinal ischemia reperfusion, decreased TH-positive neurons in the locus coeruleus, and ameliorated cognitive impairment. Similarly, DSP-4 pre-treatment alleviated neuroinflammation and improved cognitive function. Furthermore, α2-adrenergic receptor antagonist atipamezole or yohimbine administration diminished the neuroprotective effects and improved cognitive function with dexmedetomidine. Therefore, dexmedetomidine attenuated early cognitive dysfunction induced by intestinal ischemia-reperfusion injury in mice, which may be related to its anti-inflammatory effects through the LCNE system.
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http://dx.doi.org/10.1007/s11064-022-03706-wDOI Listing
August 2022

A Phase Ib Study of the Simmitecan Single Agent and in Combination With 5-Fluorouracil/Leucovorin or Thalidomide in Patients With Advanced Solid Tumor.

Front Pharmacol 2022 22;13:833583. Epub 2022 Jul 22.

Department of Gastrointestinal Oncology, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital and Institute, Beijing, China.

Simmitecan is a potent inhibitor of topoisomerase I with anti-tumor activity. This phase Ib trial was conducted to investigate the safety and anti-tumor effect of simmitecan alone or in combination with other drugs. Eligible patients with advanced solid tumor had no further standard treatment options. Patients were allocated to receive simmitecan alone, simmitecan in combination with 5-fluorouracil (5-FU)/leucovorin (LV), or simmitecan in combination with thalidomide, 14 days a cycle, until disease progression or unacceptable toxicity occurred. A total of 41 patients were enrolled, with a median age of 55 (range 29-69) years. Among them, 13 patients received simmitecan monotherapy, 10 received simmitecan + 5-FU/LV, and 18 received simmitecan + thalidomide. No dose-limiting toxicity occurred. Overall, the most common grade 3/4 adverse event (AE) was neutropenia (46.2, 70.0, and 88.9%, respectively, in simmitecan, simmitecan + 5-FU/LV, and simmitecan + thalidomide cohorts), and treatment-related severe AEs included anemia and febrile neutropenia (7.7% each in simmitecan cohort), diarrhea (10% in simmitecan +5-FU/LV cohort), and febrile neutropenia (5.6% in simmitecan + thalidomide cohort). The majority of patients (24/41, 58.3%) had progressed on prior irinotecan; nevertheless, partial response was achieved in one colorectal cancer patients treated with simmitecan + thalidomide. The disease control rates of simmitecan, simmitecan + 5-FU/LV, and simmitecan + thalidomide cohorts were 46.2, 80.0, and 61.1%, respectively. This study demonstrated a manageable safety profile of simmitecan as a single agent or as part of a combination therapy. There have not been any safety concerns with simmitecan in combination when compared to simmitecan alone. Simmitecan + 5-FU/LV regimen seemed to have a better efficacy. Nonetheless, the efficacy of this regimen needs to be further explored in the subsequent study.
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http://dx.doi.org/10.3389/fphar.2022.833583DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355729PMC
July 2022

Effects of the Chinese herbal medicine Hong Huang decoction, on myocardial injury in breast cancer patients who underwent anthracycline-based chemotherapy.

Front Cardiovasc Med 2022 22;9:921753. Epub 2022 Jul 22.

Department of Breast Disease, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.

Objective: To assess the effects of Hong Huang Decoction (HHD), a Chinese herbal medicine, on myocardial injury in breast cancer patients who underwent anthracycline (ANT)-based chemotherapy.

Methods: A total of 51 patients with breast cancer who underwent an ANT-based chemotherapy program and met the inclusion/exclusion criteria were allocated to the treatment or placebo groups using a random number generation process. Patients in the treatment group received liquid HHD twice a day. Treatment was given from 1 day prior to chemotherapy up to the end of chemotherapy (after 6 months). Participants in the placebo group received a placebo over the same schedule. Left ventricular ejection fraction (LVEF), global longitudinal strain (GLS), diagnostic markers of acute myocardial infarction [e.g., lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), and B-type natriuretic peptide (BNP)], nitric oxide (NO), superoxide dismutase (SOD), as well as pro-inflammatory cytokines [e.g., tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and human C-reactive protein (CRP)], and anti-inflammatory cytokine interleukin-10 (IL-10), were outcome measures assessed before chemotherapy, 3 and 6 months after chemotherapy.

Results: Compared to the placebo group, the GLS value was significantly higher in the treatment group (19.95 ± 1.16 vs. 19.06 ± 1.64, ≤ 0.001). Significant differences were also noted for levels of SOD (689.71 ± 203.60 vs. 807.88 ± 182.10, < 0.05), IL-6 (58.04 ± 22.06 vs. 194.20 ± 40.14, ≤ 0.001), IL-10 (237.90 ± 94.98 vs. 68.81 ± 32.92, ≤ 0.001), NO (75.05 ± 26.39 vs. 55.83 ± 19.37, ≤ 0.005), and TNF-α (301.80 ± 134.20 vs. 680.30 ± 199.60, ≤ 0.001) in the patients before chemotherapy compared to 6 months after initiating chemotherapy.

Conclusion: HHD regulated the levels of IL-6, IL-10, SOD, NO, and TNF-α. The results demonstrated that GLS is a better indicator of early myocardial injury compared to LVEF, and HHD could modulate oxidative stress to protect against ANT cardio toxicity.

Clinical Trial Registration: Chinese Clinical Trial Registry, identifier ChiCTR1900022394. Date of registration: 2019-04-09.
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http://dx.doi.org/10.3389/fcvm.2022.921753DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353583PMC
July 2022

An acid-base responsive AuI integrated contrast agent for Optical/CT double-modal imaging to detect pH change of digestive tract.

Anal Chim Acta 2022 Aug 24;1221:340119. Epub 2022 Jun 24.

Clinical Laboratory Medicine Department, College and Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi, China; Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Nanning, Guangxi, China. Electronic address:

The detection of pH change in the gastrointestinal tract (GIT) is invasive and the intestinal pH detection is even harder. Here, we develop an AuI integrated contrast agent (Au [email protected]) for noninvasive GIT imaging to detect pH change. This agent is composed of gold nanoclusters (Au NCs) and diatrizoic acid (DA) and is extremely sensitive to the acid-base response. Au [email protected] about 400 nm in size can be stable in acid solution and make the fluorescence intensity of Au NCs to drop significantly. After entering a neutral environment, Au [email protected] can rapidly form sediment, and then its CT value and fluorescence increase. Alkaline pH can trigger the release of DA from Au [email protected] to make its fluorescence intensity to recover. As entering GIT, Au [email protected] can successively outline their anatomy for optical/CT double-modal imaging under gastrointestinal motility. The variations of the fluorescence and CT images triggered by different pH are also recorded to analyze the pH change of GIT. Furthermore, the clearance rate of stable Au [email protected] in acid pH increases, which also can assist to evaluate pH value. Therefore, Au [email protected] can definitely be an excellent candidate for the noninvasive detection of pH change in GIT through optical/CT double-modal imaging.
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http://dx.doi.org/10.1016/j.aca.2022.340119DOI Listing
August 2022

The role of AMPK-Sirt1-autophagy pathway in the intestinal protection process by propofol against regional ischemia/reperfusion injury in rats.

Int Immunopharmacol 2022 Aug 4;111:109114. Epub 2022 Aug 4.

Department of Anesthesiology, The Affiliated Hospital of Southwest Medical University, Luzhou, PR China; Laboratory of Anesthesiology, Southwest Medical University, Luzhou, PR China. Electronic address:

Intestinal ischemia/reperfusion (II/R) is a clinical event associated with high morbidity and mortality. AMP-activated protein kinase (AMPK), a central cellular energy sensor, is associated with oxidative stress and inflammation. However, whether the AMPK is involved in the II/R-induced intestinal injury and the underlying mechanism is yet to be elucidated. Propofol has a protective effect on organs; yet, its specific mechanism of action remains unclear. This study explored the role of the AMPK-Sirt1-autophagy pathway in intestinal injury, and whether propofol could reduce intestinal injury and investigated the mechanisms in a rat model of II/R injury as well as a cell model (IEC-6 cells) of hypoxia/reoxygenation (H/R). Propofol, AMPK agonist (AICAR) and AMPK inhibitor (Compound C) were then administered, respectively. The histopathological changes, cell viability and apoptosis were detected. Furthermore, the levels of proinflammatory factors, the activities of oxidative stress, diamine oxidase, and signaling pathway were also analyzed. The results demonstrated that the AMPK-Sirt1-autophagy pathway of intestine was activated after II/R or H/R. Propofol could further activate the pathway, which reduced intestinal injury, inhibited apoptosis, reversed inflammation and oxidative stress, and improved the 24-hour survival rate in II/R rats in vivo, and attenuated H/R-induced IEC-6 cell injury, oxidative stress, and apoptosis in vitro, as fine as changes in AICAR treatment. Compound C abrogated the protective effect of propofol on II/R and H/R-induced injury. These results suggested a crucial effect of AMPK on the mechanism of intestinal injury and might provide a new insight into the mechanism of propofol reducing II/R injury.
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http://dx.doi.org/10.1016/j.intimp.2022.109114DOI Listing
August 2022

Effects of an inhibitor of the SHH signaling pathway on endometrial cells of patients with endometriosis.

BMC Mol Cell Biol 2022 Aug 6;23(1):37. Epub 2022 Aug 6.

Department of Gynaecology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.

Background: Endometriosis is one of the most common gynecological diseases, and seriously reduces the quality of life of patients. However, the pathogenesis of this disease is unclear. Therefore, more studies are needed to elucidate its pathogenesis. Our previous publication found that the Sonic Hedgehog (SHH) signaling pathway was activated in endometriosis. This study tested whether SHH signaling in endometrial stromal cells (ESCs) was critical for the pathogenesis of endometriosis.

Methods: To examine the effect of inhibiting the SHH signaling pathway on endometriosis, we first isolated ESCs from eutopic endometrial tissues of patients with or without endometriosis and identified the extracted cells by morphological observation and immunofluorescence. Then, we treated ESCs with the GLI inhibitor GANT61 and used CCK-8, wound healing and invasion assays to detect cell activities, such as proliferation, invasion and metastasis. Furthermore, we detected the expression of key proteins and proliferation markers of the SHH signaling pathway in the lesions of nude mice using immunochemistry.

Results: We demonstrated that higher concentrations of GANT61 decreased the proliferation rate and migration distance of ESCs. We observed that GANT61 inhibited the invasion of ESCs. In addition, blockage of the SHH signaling pathway significantly reduced cell proliferation in vitro.

Conclusions: Our study suggested that inhibition of the SHH pathway is involved in cell proliferation and invasive growth in the pathogenesis of endometriosis.
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http://dx.doi.org/10.1186/s12860-022-00426-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356504PMC
August 2022

Effects of the Separation Distance between Two Triplet States Produced from Intramolecular Singlet Fission on the Two-Electron-Transfer Process.

J Am Chem Soc 2022 Aug 5. Epub 2022 Aug 5.

Shandong Energy Group Co., Ltd., Jinan, Shandong 250014, People's Republic of China.

To harvest two triplet excitons of singlet fission (SF) via a two-electron transfer efficiently, the revelation of the key factors that influence the two-electron-transfer process is necessary. Here, by using steady-state and transient absorption/fluorescence spectroscopy, we investigated the two-electron-transfer process from the two triplet excitons of intramolecular SF (iSF) in a series of tetracene oligomers (dimer, trimer, and tetramer) with 7,7,8,8-tetracyanoquinodimethane (TCNQ) as an electron acceptor in solution. Quantitative two-electron transfer could be conducted for the trimer and tetramer, and the rate for the tetramer is faster than that for the trimer. However, the maximum efficiency of the two-electron transfer in the dimer is relatively low (∼47%). The calculation result of the free energy change (Δ) of the second-electron transfer for these three compounds (-0.024, -0.061, and -0.074 eV for the dimer, trimer, and tetramer, respectively) is consistent with the experimental observation. The much closer Δ value to zero for the dimer should be responsible for its low efficiency of the two-electron transfer. Different Δ values for these three oligomers are attributed to the different Coulomb repulsive energies between the two positive charges generated after the two-electron transfer that is caused by their various intertriplet distances. This result reveals for the first time the important effect of the Coulomb repulsive energy, which depends on the intertriplet distance, on the two-electron transfer process from the two triplet excitons of iSF.
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http://dx.doi.org/10.1021/jacs.2c03550DOI Listing
August 2022

Unveiling Interstitial Anionic Electron-Driven Ultrahigh K-Ion Storage Capacity in a Novel Two-Dimensional Electride Exemplified by ScSi.

J Phys Chem Lett 2022 Aug 5:7439-7447. Epub 2022 Aug 5.

Department of Physics and Centre for Advanced Two-Dimensional Materials, National University of Singapore, Singapore 117551, Singapore.

Two-dimensional (2D) electrides, characterized by excess interstitial anionic electron (IAE) in a crystalline 2D material, offer promising opportunities for the development of electrode materials, in particular in rechargeable metal-ion batteries applications. Although a few such potential electride materials have been reported, they generally show low metal-ion storage capacity, and the effect of IAE on the ion storage performance remains elusive so far. Here we report a novel 2D electride, [ScSi]·1e, with fascinating IAE-driven high alkali metal-ion storage capacity. In particular, its K-ion specific capacity can reach up to 1497 mA h g, higher than any previously reported 2D materials-based anodes in K-ion batteries (PIBs). The IAE in the [ScSi]·1e crystal accounts for such high capacity behavior, which can drift away and balance the charge on the metal-cation, playing a crucial role in stabilizing the metal-ion adsorption and enhancing multilayer-ions adsorption. This proposed IAE-driven storage mechanism provides an unprecedented avenue for the future design of high storage capacity electrode materials.
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http://dx.doi.org/10.1021/acs.jpclett.2c01888DOI Listing
August 2022

Perioperative administration of methylprednisolone was associated with postoperative pulmonary complications in elderly patients undergoing hip fracture surgery.

Aging Clin Exp Res 2022 Aug 4. Epub 2022 Aug 4.

Department of Anesthesiology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou, 510630, China.

Background: Postoperative pulmonary complications (PPCs) seriously affect the postoperative prognosis of elderly patients underwent hip fracture surgery. Although methylprednisolone is increasingly used, the association between perioperative methylprednisolone and PPCs is still controversial. The study aims to determine whether perioperative administration of methylprednisolone is associated with PPCs in elderly patients during hip fracture surgery.

Patients And Methods: In this retrospective cohort study, records of 584 patients (≥ 65 years) who underwent hip fracture surgery between January 2013 and October 2020 were extracted. Univariate and multivariate regression analysis were performed to identify the risk factors for PPCs. To further explore the association between administration of methylprednisolone and PPCs, 53 patients received methylprednisolone and 53 patients without methylprednisolone were matched for the confounding factors using propensity score matching (PSM) analysis. The odds ratios (OR) and 95% confidence intervals (CI) for the above variables were analyzed.

Results: The incidence of PPCs during postoperative hospitalization was 6.83% (38/556) among the elderly patients following hip fracture surgery. Patients with PPCs had higher postoperative mortality rate, longer hospital stay, more hospitalization cost, and higher incidence of cardiac arrest (all P < 0.05). Multivariate logistic regression analysis showed that age, hypertension, hypoglycemia, hypoproteinemia and perioperative methylprednisolone were independent risk factors for PPCs. Moreover, administration of methylprednisolone was significantly correlated with PPCs both before PSM adjustment (OR = 3.25; 95% CI, 1.67 to 6.33; P = 0.001) and after PSM adjustment (OR = 6.68; 95% CI, 1.40 to 31.82; P = 0.017).

Conclusion: Perioperative administration of methylprednisolone is a risk factor for PPCs in elderly patients undergoing hip fracture surgery.
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http://dx.doi.org/10.1007/s40520-022-02166-0DOI Listing
August 2022

Development of a load frame for neutron diffraction and fluorescent thermometry at cryogenic temperature.

Rev Sci Instrum 2022 Jul;93(7):073904

Key Laboratory of Mechanics on Disaster and Environment in Western China Attached to the Ministry of Education of China, Lanzhou University, Lanzhou, Gansu 730000, China.

Over the years, rapidly rising interest in the mechanical properties of various materials at low temperatures has been simulated because of the growing cryogenic applications in modern engineering fields of space technology, environmental engineering, and superconductivity engineering. Realizing in situ measurement of the internal strain and the full-field strain and the temperature distribution of related materials in a cryogenic loading environment is a significant requirement for safety assessment and related research of some new large science facility projects. Here, we present a novel cryogenic load frame, which is suitable for neutron scattering measurements of internal stress at the temperature range of 6-300 K. The loading capacity is 2500 N, and the slowest loading speed is 0.001 mm/s. By replacing the vacuum chamber sealing plate with a K9 glass window, the in situ digital image correlation strain measurement can be realized. Furthermore, fluorescence thermometry has also been investigated during a heating and cooling process without deformation. Using the present design, some typical results of the 316LN stainless steel and the YBCO tape at low temperatures were introduced.
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http://dx.doi.org/10.1063/5.0068365DOI Listing
July 2022

The effects of repetitive transcranial magnetic stimulation on standing balance and walking in older adults with age-related neurological disorders: a systematic review and meta-analysis.

J Gerontol A Biol Sci Med Sci 2022 Aug 3. Epub 2022 Aug 3.

Hebrew SeniorLife Hinda and Arthur Marcus Institute for Aging Research, Harvard Medical School, Boston, MA, United States.

Background: Considerable evidence showed that repetitive transcranial magnetic stimulation (rTMS) can improve standing balance and walking performance in older adults with age-related neurological disorders. We here thus completed a systematic review and meta-analysis to quantitatively examine such benefits of rTMS.

Methods: A search strategy based on the PICOS principle was used to obtain the literature in four databases. The screening and assessments of quality and risk of bias in the included studies were independently completed by two researchers. Outcomes included scales related to standing balance, Timed Up and Go (TUG) time, and walking speed/time/distance.

Results: Twenty-three studies consisting of 532 participants were included, and the meta-analysis was completed on 21 of these studies. The study quality was good. Compared to control, rTMS induced both short-term (≤3 days after last intervention session) and long-term (≥1 month following last intervention session) significant improvements in balance scales (e.g., Berg Balance Scale), TUG time, and walking speed/time/distance (short-term: standardized mean difference [SMD]=0.26~0.34, 95% confidence interval [CI]=0.05~0.62; long-term: SMD=0.40~0.44, 95% CI=0.04~0.79) for both PD and stroke cohorts. Subgroup analyses suggested that greater than nine sessions of high-frequency rTMS targeting primary motor cortex with greater than 3000 pulses per week can maximize such benefits. Only few mild-to-moderate adverse events/side effects were reported, which were similar between rTMS and control group.

Conclusion: The results suggest that rTMS holds promise to improve balance and walking performance in older adults with age-related neurological disorders. Future studies with more rigorous design are needed to confirm the observations in this work.
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http://dx.doi.org/10.1093/gerona/glac158DOI Listing
August 2022

TUBright: A Peptide Probe for Imaging Microtubules.

Anal Chem 2022 Aug 2. Epub 2022 Aug 2.

Center for Cell Structure and Function, Shandong Provincial Key Laboratory of Animal Resistance Biology, Collaborative Innovation Center of Cell Biology in Universities of Shandong, College of Life Sciences, Shandong Normal University, Jinan 250014, China.

assays using reconstituted microtubules have provided molecular insights into the principles of microtubule dynamics and the roles of microtubule-associated proteins. Emerging questions that further uncover the complexity in microtubule dynamics, especially those on tubulin isotypes and post-translational modifications, raise new technical challenges on how to visualize microtubules composed of tubulin purified from limited sources, primarily due to the low efficiency of the conventional tubulin labeling protocol. Here, we develop a peptide probe, termed TUBright, that labels reconstituted microtubules. TUBright, when coupled with different fluorescent dyes, provides flexible labeling of microtubules with a high signal-to-noise ratio. TUBright does not interfere with the dynamic behaviors of microtubules and microtubule-associated proteins. Therefore, TUBright is a useful tool for imaging microtubules, making it feasible to use tubulin from limited sources for answering many open questions on microtubule dynamics.
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http://dx.doi.org/10.1021/acs.analchem.2c01285DOI Listing
August 2022

Investigation of the Effectiveness of Traditional Breathing Therapy on Pulmonary Function in College Students with Obstructive Sleep Apnea.

Contrast Media Mol Imaging 2022 15;2022:1674973. Epub 2022 Jul 15.

Capital University of Physical Education and Sports, 100191, Beijing, China.

Background: Obstructive sleep apnea (OSA) is a problem that involves many body systems, but its impact on the respiratory system deserves special attention. While there are many studies investigating the use of continuous positive airway pressure (CPAP) to treat lung function in patients with sleep apnea, the lack of studies in the literature on the effects of traditional breathing therapy on lung function in patients with OSA prompted us to conduct such a study.

Objective: The present randomized trial aims to assess the effect of traditional breathing therapy on daytime sleepiness and pulmonary function in college students with OSA.

Methods: Forty college students (male) with OSA were randomly divided into two groups: the control group (CG) and the traditional breathing therapy group (TG). Daytime sleepiness symptoms in OSA are measured primarily by the Epworth Sleepiness Scale (ESS). Pulmonary function measurements included FVC, FEV1, PEE, and MEF50%. The changes in morning blood pressure (BP), including diastolic BP and systolic BP, were also recorded. Data were recorded before and after the experiment.

Results: A decrease in ESS at 12 weeks after intervention had statistical significance compared with values recorded before intervention ( < 0.05). A decrease in systolic and diastolic BP at 12 weeks after intervention had statistical significance compared with values recorded before intervention ( < 0.05). Comparisons made in terms of pulmonary functions demonstrated a statistically significant increase in 12-week postintervention values of FVC, FEV1, PEF, and MEF50% ( < 0.05).

Conclusion: Our study shows the positive effects of traditional breathing therapy on pulmonary function parameters. This suggests that traditional breathing therapy treatment in OSA patients is as effective as CPAP on pulmonary function, while there is an improvement in daytime sleepiness and a modest decline in the mean daytime systolic and diastolic BP.
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http://dx.doi.org/10.1155/2022/1674973DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307394PMC
August 2022

A multi-layer strip ionization chamber (MLSIC) device for proton pencil beam scan quality assurance.

Phys Med Biol 2022 Jul 29. Epub 2022 Jul 29.

Radiation Oncology , Washington University in Saint Louis, 4921 Parkview Place, Campus Box 8224, Saint Louis, Missouri, 63110, UNITED STATES.

Objective: Proton pencil beam scanning (PBS) treatment fields needs to be verified before treatment deliveries to ensure patient safety. In current practice, treatment beam quality assurance (QA) is measured at a few selected depths using film or a 2D detector array, which is insensitive and time-consuming. A QA device that can measure all key dosimetric characteristics of treatment beams spot-by-spot within a single beam delivery is highly desired.

Approach: We developed a multi-layer strip ionization chamber (MLSIC) prototype device that comprises of two layers of strip ionization chambers (IC) plates for spot position measurement and 64 layers of plate ionization chambers for beam energy measurement. The 768-channel strip ion chamber signal are integrated and sampled at a speed of 3.125 kHz. It has a 25.6 cm × 25.6 cm maximum measurement field size and 2 mm spatial resolution for spot position measurement. The depth resolution and maximum depth were 2.91 mm and 18.6 cm for 1.6 mm thick IC plate, respectively. The relative weight of each spot was determined from total charge by all IC detector channels.

Main Results: The MLSIC is able to measure ionization currents spot-by-spot. The depth dose measurement has a good agreement with the ground truth measured using a water tank and commercial 1D multi-layer plate chamber. It can verify the spot position, energy, and relative weight of clinical PBS beams and compared with the treatment plans.

Significance: MLSIC is a highly efficient QA device that can measure the key dosimetric characteristics of proton treatment beams spot-by-spot with a single beam delivery. It may improve the quality and efficiency of clinical proton treatments.
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http://dx.doi.org/10.1088/1361-6560/ac8593DOI Listing
July 2022

Analysis of spatial distribution characteristics and main influencing factors of heavy metals in road dust of Tianjin based on land use regression models.

Environ Sci Pollut Res Int 2022 Jul 29. Epub 2022 Jul 29.

School of Geographic and Environmental Sciences, Tianjin Normal University, A 304, Boli Building, 393 Binshui West Road, Tianjin, 300387, China.

Land use regression (LUR) models are mainly used for the simulation and prediction of conventional atmospheric pollutants. Whether the LUR models can be expanded to study more toxic and hazardous pollutants (such as heavy metals) remains to be verified. Combined with the factors of road, land use type, population, pollution enterprise, meteorology, and terrain, the LUR models were used to simulate the spatial distribution characteristics of heavy metals in road dust and determine the main influencing factors. Samples of road surface dust were collected from 144 evenly distributed points in Tianjin, China, with 108 modelling points and 36 verification points. The R values of the LUR models of Cd, Cr, Cu, Ni, and Pb contents were 0.301, 0.412, 0.399, 0.496, and 0.377, and their error rates were 2.72%, 4.96%, 4.64%, 8.91%, and 4.94%, respectively. The error rates of the kriging interpolation models were 3.33%, 6.50%, 5.14%, 18.30%, and 22.87%, which were all greater than those of the LUR models. The estimation effect of the LUR models was more refined than that of the kriging interpolation models. The contents of most heavy metals (except Ni) in road dust of the central area in Tianjin were generally higher than those of the surrounding areas. The heavy metal contents in road dust of Tianjin were mainly affected by road variables and meteorological variables. The LUR models were suitable for small-scale spatial prediction of heavy metals in urban road dust within urban areas.
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http://dx.doi.org/10.1007/s11356-022-22151-4DOI Listing
July 2022

Effect of ASA on the risk of cerebrovascular ischemic events in patients with PFO.

Ann Clin Transl Neurol 2022 Jul 27. Epub 2022 Jul 27.

Department of Ultrasound, The First College of Clinical Medical Sciences, China Three Gorges University, Yichang, Hubei Province, China.

Background: Whether atrial septal aneurysm (ASA) increases the risk of cerebrovascular ischemic events in patients with patent foramen ovale (PFO) remains controversial.

Objective: We constructed a detailed meta-analysis to assess the effect of ASA on risk of cerebrovascular ischemic events in patients with PFO.

Methods: Randomized controlled trials (RCTs) and observational studies (cohort studies and case-control studies) that compared PFO-ASA against PFO alone were included. Pooled odds ratios (OR) estimates and 95% CI were calculated using the fixed-effect and random-effect models.

Results: Four RCTs and twelve observational studies (five cohort studies and seven case-control studies) contributed to the meta-analysis. The pooled results of case-control studies showed that ASA increased the risk of cerebrovascular ischemic events in patients with PFO (fixed-effect model: OR = 3.69; 95% CI: 2.67-5.09; p < 0.01, random-effect model: OR = 3.63; 95% CI: 2.51-5.24; p < 0.01). However, poole results from RCTs (fixed-effect model: OR = 1.24; 95% CI: 0.78-1.95; p = 0.36, random-effect model: OR = 1.27; 95% CI: 0.78-2.08; p = 0.34) and cohort studies (fixed-effect model: OR = 1.35; 95% CI: 0.81-2.23; p = 0.25, random-effect model: OR = 1.40; 95% CI: 0.84-2.33; p = 0.20) found no evidence. Overall analysis showed that ASA increased the risk of cerebrovascular ischemic events (fixed-effect model: OR = 2.30; 95% CI: 1.84-2.87; p < 0.01, random-effect model: OR = 2.11; 95% CI: 1.48-3.01; p < 0.01). The sensitivity analysis confirmed the stability of all results.

Conclusions: Although case-control studies support ASA to increase the risk of cerebrovascular ischemic events in patients with PFO, RCTs and cohort studies challenged the credibility. Further prospective studies are needed to confirm the effect of ASA on patients with PFO.
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http://dx.doi.org/10.1002/acn3.51638DOI Listing
July 2022

Human Grasp Mechanism Understanding, Human-Inspired Grasp Control and Robotic Grasping Planning for Agricultural Robots.

Sensors (Basel) 2022 Jul 13;22(14). Epub 2022 Jul 13.

College of Artificial Intelligence, Nanjing Agricultural University, Nanjing 210031, China.

As the end execution tool of agricultural robots, the manipulator directly determines whether the grasping task can be successfully completed. The human hand can adapt to various objects and achieve stable grasping, which is the highest goal for manipulator design and development. Thus, this study combines a multi-sensor fusion tactile glove to simulate manual grasping, explores the mechanism and characteristics of the human hand, and formulates rational grasping plans. According to the shape and size of fruits and vegetables, the grasping gesture library is summarized to facilitate the matching of optimal grasping gestures. By analyzing inter-finger curvature correlations and inter-joint pressure correlations, we investigated the synergistic motion characteristics of the human hand. In addition, the force data were processed by the wavelet transform algorithms and then the thresholds for sliding detection were set to ensure robust grasping. The acceleration law under the interaction with the external environment during grasping was also discussed, including stable movement, accidental collision, and placement of the target position. Finally, according to the analysis and summary of the manual gripping mechanism, the corresponding pre-gripping planning was designed to provide theoretical guidance and ideas for the gripping of robots.
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http://dx.doi.org/10.3390/s22145240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9324230PMC
July 2022

Highly Sensitive Plasmonic Waveguide Biosensor Based on Phase Singularity-Enhanced Goos-Hänchen Shift.

Biosensors (Basel) 2022 Jun 26;12(7). Epub 2022 Jun 26.

XLIM Research Institute, UMR 7252 CNRS/University of Limoges, 123 Avenue Albert Thomas, 87060 Limoges, France.

The detection for small molecules with low concentrations is known to be challenging for current chemical and biological sensors. In this work, we designed a highly sensitive plasmonic biosensor based on the symmetric metal cladding plasmonic waveguide (SMCW) structure for the detection of biomolecules. By precisely designing the configuration and tuning the thickness of the guiding layer, ultra-high order modes can be excited, which generates a steep phase change and a large position shift from the Goos-Hänchen effect (with respect to refractive index changes). This position shift is related to the sharpness of the optical phase change from the reflected signal of the SPR sensing substrate and can be directly measured by a position sensor. Based on our knowledge, this is the first experimental study done using this configuration. Experimental results showed a lateral position signal change > 90 µm for glycerol with a sensitivity figure-of-merit of 2.33 × 10 µm/RIU and more than 15 µm for 10 M biotin, which is a low molecular weight biomolecule (less than 400 Da) and difficult to be detected with traditional SPR sensing techniques. Through integrating the waveguide with a guiding layer, a strong improvement in the electric field, as well as sensitivity have been achieved. The lateral position shift has been further improved from 14.17 µm to 284 µm compared with conventional SPR substrate with 50 nm gold on single side. The as-reported sensing technique allows for the detection of ultra-small biological molecules and will play an important role in biomedical and clinical diagnostics.
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http://dx.doi.org/10.3390/bios12070457DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9312834PMC
June 2022

Selenoprotein K Is Essential for the Migration and Phagocytosis of Immature Dendritic Cells.

Antioxidants (Basel) 2022 Jun 27;11(7). Epub 2022 Jun 27.

Key Laboratory of Infectious Immune and Antibody Engineering of Guizhou Province, Cellular Immunotherapy Engineering Research Center of Guizhou Province, School of Biology and Engineering/School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, China.

Selenoprotein K (SELENOK) is an endoplasmic reticulum stress (ERS)-regulated protein required for the calcium (Ca) flux-mediated migration of T cells and neutrophils, and the migration and phagocytosis of macrophages and microglia. However, the effect of SELENOK on the regulation of the immune function of dendritic cells (DCs), including immature DCs (imDCs) and mature DCs (mDCs), is still unclear. In this study, imDCs prepared from SELENOK knockout mice were used to evaluate the effect of SELENOK on the migration and phagocytosis of imDCs. The results showed that ERS-induced downregulation of imDCs phenotypic markers led to a reduction in Ras homolog gene family member A (RhoA)-dependent migration and enhanced Ca/CD205-mediated phagocytosis. SELENOK deficiency-induced upregulation of selenoprotein S (SELENOS) attenuated ERS levels in imDCs. An increase in Ca levels resulted in increased migration and decreased phagocytosis with or without ERS conditions. The migration was RhoA-dependent, and Ca or CD205 was associated with regulating phagocytosis in imDCs. Our study found that SELENOK is required for imDC migration and phagocytosis.
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http://dx.doi.org/10.3390/antiox11071264DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9311522PMC
June 2022

Targeting Undruggable Transcription Factors with PROTACs: Advances and Perspectives.

J Med Chem 2022 Aug 26;65(15):10183-10194. Epub 2022 Jul 26.

Institute of Biomedical Sciences, College of Life Sciences, Shandong Normal University, Jinan, Shandong 250014, China.

Dysregulation of transcription factors has been implicated in a variety of human diseases. However, these proteins have traditionally been regarded as undruggable and only a handful of them have been successfully targeted by conventional small molecules. Moreover, the development of intrinsic and acquired resistance has hampered the clinical use of these agents. Over the past years, proteolysis-targeting chimeras (PROTACs) have shown great promise because of their potential for overcoming drug resistance and their ability to target previously undruggable proteins. Indeed, several small molecule-based PROTACs have demonstrated superior efficacy in therapy-resistant metastatic cancers. Nevertheless, it remains challenging to identify ligands for the majority of transcription factors. Given that transcription factors recognize short DNA motifs in a sequence-specific manner, multiple novel approaches exploit DNA motifs as warheads in PROTAC design for the degradation of aberrant transcription factors. These PROTACs pave the way for targeting undruggable transcription factors with potential therapeutic benefits.
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http://dx.doi.org/10.1021/acs.jmedchem.2c00691DOI Listing
August 2022

LINC00839 promotes colorectal cancer progression by recruiting RUVBL1/Tip60 complexes to activate NRF1.

EMBO Rep 2022 Jul 25:e54128. Epub 2022 Jul 25.

Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, China.

The long noncoding RNA LINC00839 has been shown to be involved in the progression of some cancer types, such as bladder cancer, prostate cancer, breast cancer, and neuroblastoma. However, if LINC00839 has roles in colorectal cancer (CRC), it has not been elucidated so far. Here, we focus on the biological role and involved mechanisms of LINC00839 in CRC. We show that LINC00839 is selectively upregulated in CRC and locates to the nucleus. High expression of LINC00839 is associated with poor outcomes in CRC patients. Functional experiments show that LINC00839 promotes CRC proliferation, invasion, and metastasis in vitro and in vivo. Mechanistically, LINC00839 recruits Ruvb1 to the Tip60 complex and increases its acetylase activity. LINC00839 guides the complex to the NRF1 promoter and promotes acetylation of lysines 5 and 8 of histones H4, thereby upregulating the expression of NRF1. Subsequently, NRF1 activates mitochondrial metabolism and biogenesis, thereby promoting CRC progression. In summary, our study reports on a mechanism by which LINC00839 positively regulates NRF1, thus promoting mitochondrial metabolism and biogenesis, as well as CRC progression.
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http://dx.doi.org/10.15252/embr.202154128DOI Listing
July 2022

Path Planning and Trajectory Tracking for Automatic Guided Vehicles.

Comput Intell Neurosci 2022 14;2022:8981778. Epub 2022 Jul 14.

Shandong Shifeng (Group) Company Ltd, Liaocheng 252000, Shandong, China.

Automated guided vehicle technology has become a hot area of scientific research due to its increasing use in manufacturing and logistics. Its main features are programming and control, remote computer eye tracking, command receiving and execution, autonomous route planning, and autonomous driving execution of tasks, with the advantages of high intelligence and flexibility. In this work, a simple vehicle model is used to study the route planning and tracking control of automatic guided vehicles. This paper uses wireless communication to find the optimal route planning problem. Using geometric methods, we develop a model of the working environment of the mobile automatic guided vehicle and develop a route finding algorithm. Based on the kinematic model, an advanced routing controller is designed to conduct experimental simulation of two trajectories and verify the effectiveness of the trajectory tracking controller. When the time is after 2 s, the position error is almost completely zero. In the path planning, when the number of iterations is greater than 10, the path length remains constant, verifying the effectiveness of the method in this paper.
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http://dx.doi.org/10.1155/2022/8981778DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9303090PMC
July 2022

Deformable CT image registration via a dual feasible neural network.

Med Phys 2022 Jul 23. Epub 2022 Jul 23.

Department of Radiation Oncology and Winship Cancer Institute, Emory University, Atlanta, Georgia, USA.

Purpose: A quality assurance (QA) CT scans are usually acquired during cancer radiotherapy to assess for any anatomical changes, which may cause an unacceptable dose deviation and therefore warrant a replan. Accurate and rapid deformable image registration (DIR) is needed to support contour propagation from the planning CT (pCT) to the QA CT to facilitate dose volume histogram (DVH) review. Further, the generated deformation maps are used to track the anatomical variations throughout the treatment course and calculate the corresponding accumulated dose from one or more treatment plans.

Methods: In this study, we aim to develop a deep learning (DL)-based method for automatic deformable registration to align the pCT and the QA CT. Our proposed method, named dual-feasible framework, was implemented by a mutual network that functions as both a forward module and a backward module. The mutual network was trained to predict two deformation vector fields (DVFs) simultaneously, which were then used to register the pCT and QA CT in both directions. A novel dual feasible loss was proposed to train the mutual network. The dual-feasible framework was able to provide additional DVF regularization during network training, which preserves the topology and reduces folding problems. We conducted experiments on 65 head-and-neck cancer patients (228 CTs in total), each with 1 pCT and 2-6 QA CTs. For evaluations, we calculated the mean absolute error (MAE), peak-signal-to-noise ratio (PSNR), structural similarity index (SSIM), target registration error (TRE) between the deformed and target images and the Jacobian determinant of the predicted DVFs.

Results: Within the body contour, the mean MAE, PSNR, SSIM, and TRE are 122.7 HU, 21.8 dB, 0.62 and 4.1 mm before registration and are 40.6 HU, 30.8 dB, 0.94, and 2.0 mm after registration using the proposed method. These results demonstrate the feasibility and efficacy of our proposed method for pCT and QA CT DIR.

Conclusion: In summary, we proposed a DL-based method for automatic DIR to match the pCT to the QA CT. Such DIR method would not only benefit current workflow of evaluating DVHs on QA CTs but may also facilitate studies of treatment response assessment and radiomics that depend heavily on the accurate localization of tissues across longitudinal images.
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http://dx.doi.org/10.1002/mp.15875DOI Listing
July 2022

Effects of Clostridium butyricum Capsules Combined with Rosuvastatin on Intestinal Flora, Lipid Metabolism, Liver Function and Inflammation in NAFLD Patients.

Cell Mol Biol (Noisy-le-grand) 2022 Feb 28;68(2):64-69. Epub 2022 Feb 28.

Tongren Hospital Shanghai Jiaotong University of Medical.

The objective of this study was to investigate the effects of Clostridium butyricum capsules combined with rosuvastatin on the intestinal flora, lipid metabolism, liver function and inflammation in patients with nonalcoholic fatty liver disease (NAFLD). For this purpose, a total of 96 patients with NAFLD were selected as research subjects and randomly divided into a control group (n=48) and an observation group (n=48). The Control group was treated with rosuvastatin, based on which observation group received Clostridium butyricum capsule treatment. The efficacy in the two groups of patients was compared, and the intestinal flora, lipid metabolism, liver function and inflammation were observed. Results showed that the efficacy in the observation group was significantly better than that in the control group (p<0.05). After treatment, the content of Eubacterium rectale in the observation group was lower than that in the control group, while the content of Bacteroides thetaiotaomicron and Bifidobacteria was notably higher than that in the control group (p<0.05). Moreover, the observation group had remarkably lower levels of total cholesterol (TC), triglyceride (TG), free fatty acids (FFA), total bilirubin (TBIL), direct bilirubin (DBIL), alanine aminotransferase (ALT), aspartate aminotransferase (AST), procollagen III peptide (PIIIP), collagen-IV (C-IV), hyaluronicacid (HA) and laminin (LN) as well as lower levels of tumor necrosis factor-alpha (TNF-α), catabolite activator protein (CAP) and interleukin-6 (IL-6) in serum than the control group (p<0.05). It was concluded that Clostridium butyricum capsules combined with rosuvastatin can effectively improve intestinal flora imbalance, reduce blood lipid levels, and alleviate liver fibrosis and liver function damage in the treatment of NAFLD, so it is of therapeutic value.
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http://dx.doi.org/10.14715/cmb/2022.68.2.10DOI Listing
February 2022

mmu-lncRNA 121686/hsa-lncRNA 520657 induced by METTL3 drive the progression of AKI by targeting miR-328-5p/HtrA3 signaling axis.

Mol Ther 2022 Jul 21. Epub 2022 Jul 21.

Department of Emergency Medicine, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, People's Republic of China; Emergency Medicine and Difficult Diseases Institute, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, People's Republic of China; Hunan Clinical Medical Research Center for Acute Organ Injury and Repair, Changsha, Hunan 410011, People's Republic of China. Electronic address:

The pathogenesis of acute kidney injury (AKI) is still not fully understood, and effective interventions are lacking. Here, we explored whether methyltransferase 3 (METTL3) was involved in the progression of AKI via regulation of cell death. We reported that PT(proximal tubule)-METTL3-knockout (KO) noticeably suppressed ischemic-induced AKI via inhibition of renal cell apoptosis. Furthermore, we also found that the expression of mmu-long non-coding RNA (lncRNA) 121686 was upregulated in antimycin-treated Boston University mouse proximal tubule (BUMPT) cells and a mouse ischemia-reperfusion (I/R)-induced AKI model. Functionally, mmu-lncRNA 121686 could promote I/R-induced mouse renal cell apoptosis. Mechanistically, mmu-lncRNA 121686 acted as a competing endogenous RNA (ceRNA) to prevent microRNA miR-328-5p-mediated downregulation of high-temperature requirement factor A 3 (Htra3). PT-mmu-lncRNA 121686-KO mice significantly ameliorated the ischemic-induced AKI via the miR-328-5p/HtrA3 axis. In addition, hsa-lncRNA 520657, homologous with lncRNA 121686, sponged miR-328-5p and upregulated Htra3 to promote I/R-induced human renal cell apoptosis. Interestingly, we found that mmu-lncRNA 121686/hsa-lncRNA 520657 upregulation were dependent on METTL3 via N-methyladenosine (mA) modification. The mmu-lncRNA 121686/miR-328-5p or hsa-lncRNA 520657/miR-328-5p /HtrA3 axis was induced in vitro by METTL3 overexpression; in contrast, this effect was attenuated by METTL3 small interfering RNA (siRNA). Furthermore, we found that PT-METTL3-KO or METTL3 siRNA significantly suppressed ischemic, septic, and vancomycin-induced AKI via downregulation of the mmu-lncRNA 121686/miR-328-5p/HtrA3 axis. Taken together, our data indicate that the METTL3/mmu-lncRNA 121686/hsa-lncRNA 520657/miR-328-5p/HtrA3 axis potentially acts as a therapeutic target for AKI.
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http://dx.doi.org/10.1016/j.ymthe.2022.07.014DOI Listing
July 2022

Automated interpretation of the pulmonary function test by a portable spirometer in Chinese adults.

Clin Respir J 2022 Jul 22. Epub 2022 Jul 22.

Department of Respiratory and Critical Care Medicine, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Introduction: A portable spirometer is a promising alternative to a traditional pulmonary function test (PFT) spirometer for respiratory function evaluation.

Objectives: This study aimed to investigate the accuracy of automated interpretation of the PFT measured by a portable Yue Cloud spirometer in Chinese adults.

Methods: The PFT was performed to evaluate subjects prospectively enrolled at Ruijin Hospital (n = 220). A Yue Cloud spirometer and a conventional Jaeger MasterScreen device were applied to each patient with a 20-min quiescent period between each measurement. Pulmonary function parameters, including forced vital capacity (FVC), forced expiratory volume in the first second (FEV1), peak expiratory flow (PEF), maximal expiratory flow at 25%, 50%, and 75% of the FVC (MEF25, MEF50, and MEF75, respectively), and maximal mid-expiratory flow (MMEF), were compared by correlation analyses and Bland-Altman methods. The Yue Cloud spirometer automatically interpreted the PFT results, and a conventional strategy was performed to interpret the PFT results obtained by the Jaeger machine. Concordance of the categorization of pulmonary dysfunction, small airway dysfunction, and severity was analyzed by the kappa (κ) statistic.

Results: Significantly similar correlations of all variables measured with the two spirometers were observed (all p < 0.001). No significant bias was observed in any of the measured spirometer variables. A satisfactory concordance of pulmonary function and severity classification was observed between the automated interpretation results obtained with the Yue Cloud spirometer vs. a conventional spirometer interpretation strategy (all κ > 0.80).

Conclusion: The portable Yue Cloud spirometer not only yields reliable measurements of pulmonary function but also can automatically interpret the PFT results.
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http://dx.doi.org/10.1111/crj.13525DOI Listing
July 2022

Evaluating the value of superoxide dismutase in anti-MDA5-positive dermatomyositis associated with interstitial lung disease.

Rheumatology (Oxford) 2022 Jul 22. Epub 2022 Jul 22.

Department of Rheumatology and Immunology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Objective: This study aimed to investigate the relationship between serum superoxide dismutase (SOD) and interstitial lung disease (ILD) among patients with anti-melanoma differentiation-associated gene 5 antibody-positive dermatomyositis (anti-MDA5-positive DM).

Method: In this retrospective study, serum SOD of 90 health checkups were tested in our hospital. 94 hospitalised patients with anti-MDA5-positive DM having ILD. Their serum SOD, serum ferritin, and autoantibody levels were determined, and lung high-resolution computed tomography was performed.

Results: The serum SOD level was significantly lower in the anti-MDA5-positive DM group compared with the control group. The SOD level was significantly lower in patients positive for both anti-MDA5 antibodies and anti-Ro-52 antibodies than in those positive for only anti-MDA5 antibodies before treatment. The SOD level was significantly lower in the higher serum ferritin group compared with the lower serum ferritin group before treatment. After treatment, the serum SOD level decreased in patients with exacerbation of ILD, while it increased in those with alleviated ILD. The SOD level was significantly lower in the death group than in the survival group before treatment.

Conclusions: In patients with anti-MDA5-positive DM, (1) the low SOD level before treatment indicated the presence of oxidative stress in the disease; (2) the serum SOD level was affected by anti-Ro-52 antibodies and ferritin; (3) there is a close relationship between serum SOD level and ILD among patients with anti-MDA5-positive DM, suggesting that SOD might serve as an effective indicator to evaluate the changes in ILD in these patients; and (4) the low SOD level is an important indicator of poor prognosis in these patients, which deserves attention from rheumatologists.
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http://dx.doi.org/10.1093/rheumatology/keac429DOI Listing
July 2022

High-efficiency synthesis of red carbon dots using machine learning.

Chem Commun (Camb) 2022 Aug 9;58(64):9014-9017. Epub 2022 Aug 9.

Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Computer and Information Science, Southwest University, Chongqing, 400715, P. R. China.

Due to their excellent optical properties, red carbon dots (CDs) have been widely used in cell imaging and biomedical therapy. However, the efficiency of red CD synthesis is deficient, and the synthesis cost is high. Here, we propose an efficient synthesis method based on machine learning to assist researchers in synthesizing red fluorescent CDs. This strategy can quickly and efficiently predict the predesigned conditions of CD synthesis. It avoids invalid synthetic experiments and improves the efficiency of red CD synthesis.
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http://dx.doi.org/10.1039/d2cc03473eDOI Listing
August 2022

Apoptosis-Related Gene-Mediated Cell Death Pattern Induces Immunosuppression and Immunotherapy Resistance in Gastric Cancer.

Front Genet 2022 5;13:921163. Epub 2022 Jul 5.

Department of Gastrointestinal Surgery, Maoming People's Hospital, Maoming, China.

Apoptosis is a type of cell death, which can produce abundant mediators to modify the tumor microenvironment. However, relationships between apoptosis, immunosuppression, and immunotherapy resistance of gastric cancer (GC) remain unclear. Gene expression data and matching clinical information were extracted from TCGA-STAD, GSE84437, GSE34942, GSE15459, GSE57303, ACRG/GSE62254, GSE29272, GSE26253, and IMvigor210 datasets. A consensus clustering analysis based on six apoptosis-related genes (ARGs) was performed to determine the molecular subtypes, and then an apoptosisScore was constructed based on differentially expressed and prognostic genes between molecular subtypes. Estimate R package was utilized to calculate the tumor microenvironment condition. Kaplan-Meier analysis and ROC curves were performed to further confirm the apoptosisScore efficacy. Based on six ARGs, two molecular subgroups with significantly distinct survival and immune cell infiltration were identified. Then, an apoptosisScore was built to quantify the apoptosis index of each GC patient. Next, we investigated the correlations between the clinical characteristics and apoptosisScore using logistic regression. Multivariate Cox analysis shows that low apoptosisScore was an independent predictor of poor overall survival in TCGA and ACRG datasets, and was associated with the higher pathological stage. Meanwhile, low apoptosisScore was associated with higher immune cell, higher ESTIMATEScore, higher immuneScore, higher stromalScore, higher immune checkpoint, and lower tumorpurity, which was consistent with the "immunity tidal model theory". Importantly, low apoptosisScore was sensitive to immunotherapy. In addition, GSEA indicated that several gene ontology and Kyoto Encyclopedia of Genes and Genomes items associated with apoptosis, several immune-related pathways, and JAK-STAT signal pathway were considerably enriched in the low apoptosisScore phenotype pathway. Our findings propose that low apoptosisScore is a prognostic biomarker, correlated with immune infiltrates, and sensitivity to immunotherapy in GC.
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http://dx.doi.org/10.3389/fgene.2022.921163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9295743PMC
July 2022
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