Publications by authors named "Jun Zhao"

2,459 Publications

  • Page 1 of 1

[Analysis on the characteristics of 837 patients with post-traumatic lower limb deformities from QIN Sihe Orthopaedic Surgery Database between May 25, 1978 and December 31, 2020].

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2022 Aug;36(8):1011-1014

Department of Orthopaedics, Rehabilitation Hospital of the National Research Center for Rehabilitation Technical Aids, Beijing, 100176, P. R. China.

Objective: To summarize and analyze the characteristics and treatment strategies of post-traumatic lower limb deformity based on QIN Sihe Orthopaedic Surgery Database.

Methods: A clinical data of 837 patients with post-traumatic lower limb deformities treated by orthopaedic surgery between May 25, 1978 and December 31, 2020 in QIN Sihe Orthopaedic Surgery Database were analyzed retrospectively. The information of the patient's gender, age at the time of surgery, region of origin, cause of trauma, deformity side, orthopedic surgery related information (operation time, location, type, and fixation method after operation) were summarized and analyzed.

Results: All patients came from 32 provinces, municipalities, autonomous regions, and Taiwan in China. Among them, 551 cases (65.83%) were male and 286 cases (34.17%) were female. The age of the patients at the time of surgery was 3-84 years old, with an average of 27.6 years old, and the most patients were 16-45 years old (559 cases, 66.78%). The main cause of trauma was traffic accident injury (639 cases, 76.34%). The deformity mainly involved unilateral limbs, including 394 cases (47.07%) on the left side and 376 cases (44.92%) on the right side. The most patients were admitted between 2008 and 2017, accounting for 53.05% (444/837). All patients were operated on one or more sites (1 048 sites), among which ankle and toe surgery were the most, accounting for 48.38% (507/1 048). The patients received 1204 surgeries including tendon lengthening and soft tissue contracture release, . Orthopedic surgery combined with bone external fixation was used in 624 cases (467 cases of Ilizarov external fixation and 157 cases of combined external fixation), and plaster or brace external fixation was used in 213 cases.

Conclusion: Post-traumatic lower extremity deformity patients have a large proportion of males, with a wide geographical distribution, involving various parts of the lower extremities, and most commonly in the foot and ankle. Orthopedic surgery combined with bone external fixation (Ilizarov technique) is the main methods for correction and functional reconstruction of post-traumatic lower limb deformity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.7507/1002-1892.202204098DOI Listing
August 2022

Comparison of [Ga]Ga-FAPI and [F]FDG uptake in patients with gastric signet-ring-cell carcinoma: a multicenter retrospective study.

Eur Radiol 2022 Aug 17. Epub 2022 Aug 17.

Department of Nuclear Medicine & Minnan PET Center, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China.

Objective: In this study, we investigated the role of [Ga]Ga-FAPI PET imaging in the detection of primary and metastatic gastric signet-ring-cell carcinoma (GSRCC) and compared with [F]FDG PET.

Methods: This retrospective multicenter analysis included 34 patients with histologically confirmed GSRCCs from four medical centers. The maximum standard uptake value (SUV), tumor-to-background ratio (TBR), and diagnostic accuracy were compared between the two modalities. [F]FDG and [Ga]Ga-FAPI uptakes were compared by using the Wilcoxon signed-rank test. McNemar's test was used to compare the diagnostic accuracy between the two techniques.

Results: Data were analyzed from 27 paired PET/CT and 7 paired PET/MRI scans for 34 GSRCC patients (16 men and 18 women) who had a median age of 51 years (range: 25-85 years). [Ga]Ga-FAPI PET showed higher SUV and TBR values than did [F]FDG PET in the primary tumors (SUV: 5.2 vs. 2.2, p = 0.001; TBR: 7.6 vs. 1.3, p < 0.001), involved lymph nodes (SUV: 6.8 vs. 2.5, p < 0.001; TBR: 5.8 vs. 1.3, p < 0.001), and bone and visceral metastases (SUV: 6.5 vs. 2.4, p < 0.001; TBR: 6.3 vs. 1.3, p < 0.001). In diagnostic performance, [Ga]Ga-FAPI PET exhibited higher sensitivity than [F]FDG PET for detecting primary tumors (73% [16/22] vs. 18% [4/22], p < 0.001), local recurrences (100% [7/7] vs. 29% [2/7], p = 0.071), lymph node metastases (77% [59/77] vs. 23% [18/77], p < 0.001), and distant metastases (93% [207/222] vs. 39% [86/222], p < 0.001).

Conclusion: The results from this multicenter retrospective analysis justify the clinical use of [Ga]Ga-FAPI tracers for GSRCC diagnosis and staging.

Key Points: • [Ga]Ga-FAPI PET/CT is a promising imaging modality for the detection of primary and metastatic disease and has implications for TNM staging in GSRCC. • In this multicenter study of 34 patients with GSRCC, [Ga]Ga-FAPI PET exhibited greater radiotracer uptake, tumor-to-background ratios, and diagnostic accuracy than [F]FDG PET for detecting primary/recurrent tumors and metastatic lesions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00330-022-09084-9DOI Listing
August 2022

Clinical value and molecular mechanism of AQGPs in different tumors.

Med Oncol 2022 Aug 16;39(11):174. Epub 2022 Aug 16.

Department of Urology, The First Affiliated Hospital of Dalian Medical University, Dalian, 116021, China.

Aquaglyceroporins (AQGPs), including AQP3, AQP7, AQP9, and AQP10, are transmembrane channels that allow small solutes across biological membranes, such as water, glycerol, HO, and so on. Increasing evidence suggests that they play critical roles in cancer. Overexpression or knockdown of AQGPs can promote or inhibit cancer cell proliferation, migration, invasion, apoptosis, epithelial-mesenchymal transition and metastasis, and the expression levels of AQGPs are closely linked to the prognosis of cancer patients. Here, we provide a comprehensive and detailed review to discuss the expression patterns of AQGPs in different cancers as well as the relationship between the expression patterns and prognosis. Then, we elaborate the relevance between AQGPs and malignant behaviors in cancer as well as the latent upstream regulators and downstream targets or signaling pathways of AQGPs. Finally, we summarize the potential clinical value in cancer treatment. This review will provide us with new ideas and thoughts for subsequent cancer therapy specifically targeting AQGPs.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s12032-022-01766-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9381609PMC
August 2022

Distributions and relationships of virio- and picoplankton in the epi-, meso- and bathypelagic zones of the Amundsen Sea, West Antarctica during the austral summer.

Front Microbiol 2022 27;13:941323. Epub 2022 Jul 27.

College of Marine Life Sciences, Key Lab of Polar Oceanography and Global Ocean Change, Institute of Evolution and Marine Biodiversity, and Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, China.

Virioplankton and picoplankton are the most abundant marine biological entities on earth and mediate biogeochemical cycles in the Southern Ocean. However, understanding of their distribution and relationships with environmental factors is lacking. Here, we report on their distribution and relationships with environmental factors at 48 stations from 112.5° to 150°W and 67° to 75.5°S in the Amundsen Sea of West Antarctica. The epipelagic stations were grouped into four clusters based on the virio- and picoplankton composition and abundance. Clusters three and four, which were associated with the ice-edge blooms in the coastal and Amundsen Sea Polynya (ASP) areas, had high abundances of autotrophic picoeukaryotes; this resulted in subsequent high abundances of heterotrophic prokaryotes and viruses. Cluster two stations were in open oceanic areas, where the abundances of autotrophic and heterotrophic picoplankton were low. Cluster one stations were located between the areas of blooms and the oceanic areas, which had a low abundance of heterotrophic prokaryotes and picoeukaryotes and a high abundance of virioplankton. The abundance of viruses was significantly correlated with the abundances of autotrophic picoeukaryotes and Chl- concentration in oceanic areas, although this reflected a time-lag with autotrophic picoeukaryote and heterotrophic prokaryotes abundances in ice-edge bloom areas. The upwelling of Circumpolar Deep Water (CDW) might have induced the high abundance of autotrophic picoeukaryotes in the epipelagic zone, and the sinking particulate organic carbon (POC) might have induced the high abundance of heterotrophic prokaryotes and virioplankton in the meso- and bathypelagic zones. This study shows that the summer distribution of virio- and picoplankton in the Amundsen Sea of West Antarctica was mainly controlled by upwelling of the CDW and the timing of ice-edge blooms.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2022.941323DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9363919PMC
July 2022

Advances in nuclear medicine-based molecular imaging in head and neck squamous cell carcinoma.

J Transl Med 2022 Aug 12;20(1):358. Epub 2022 Aug 12.

Shanghai Fourth People's Hospital, and School of Medicine, Tongji University, Shanghai, China.

Head and neck squamous cell carcinomas (HNSCCs) are often aggressive, making advanced disease very difficult to treat using contemporary modalities, such as surgery, radiation therapy, and chemotherapy. However, targeted therapy, e.g., cetuximab, an epidermal growth factor receptor inhibitor, has demonstrated survival benefit in HNSCC patients with locoregional failure or distant metastasis. Molecular imaging aims at various biomarkers used in targeted therapy, and nuclear medicine-based molecular imaging is a real-time and non-invasive modality with the potential to identify tumor in an earlier and more treatable stage, before anatomic-based imaging reveals diseases. The objective of this comprehensive review is to summarize recent advances in nuclear medicine-based molecular imaging for HNSCC focusing on several commonly radiolabeled biomarkers. The preclinical and clinical applications of these candidate imaging strategies are divided into three categories: those targeting tumor cells, tumor microenvironment, and tumor angiogenesis. This review endeavors to expand the knowledge of molecular biology of HNSCC and help realizing diagnostic potential of molecular imaging in clinical nuclear medicine.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12967-022-03559-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9373390PMC
August 2022

Comparisons of Fertility Outcomes Following a Salpingectomy and a Salpingotomy with or Without Suturing for a Tubal Ectopic Pregnancy.

Int J Womens Health 2022 4;14:1007-1013. Epub 2022 Aug 4.

Department of Obstetrics and Gynecology, the Third Hospital of Hebei Medical University, Shijiazhuang City, 050051, People's Republic of China.

Objective: This study aimed to compare fertility outcomes in patients who underwent different laparoscopic surgeries and to determine the most beneficial surgical treatment for tubal ectopic pregnancy (EP) patients who wished to become pregnant in the future.

Methods: In this retrospective study, patients aged 18-36 years, who had been diagnosed with a tubal EP in our hospital, were enrolled. Based on the treatment methods, the patients were divided into three groups, ie a salpingectomy group, a salpingotomy with suturing group and a salpingotomy without suturing group. The follow-up time for the enrolled patients was more than three years. Serum beta (β)-human chorionic gonadotropin (β-HCG) levels were monitored after surgery. The intrauterine pregnancy (IUP) rate at 12, 24 and 36 months postoperatively, and recurrent EP rate were compared.

Results: The median time for β-HCG level returning to normal in the salpingectomy group was much shorter than in the salpingotomy with suturing or salpingotomy without suturing groups (P < 0.001). The 12, 24 and 36-month IUP rates among the three groups were not significantly different. A recurrent EP was found in only one patient in the salpingotomy with suturing group and two patients in the salpingotomy without suturing group.

Conclusion: In this study, we found that salpingectomy and salpingotomy with or without suturing methods reflected no significant differences in fertility outcomes for tubal EP patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IJWH.S367948DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359707PMC
August 2022

Mfn2 is responsible for inhibition of the RIG-I/IRF7 pathway and activation of NLRP3 inflammasome in Seneca Valley virus-infected PK-15 cells to promote viral replication.

Front Immunol 2022 25;13:955671. Epub 2022 Jul 25.

College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.

Seneca Valley virus (SVV), a non-enveloped positive single-stranded virus can cause vesicular disease in swine. However, the mechanisms by which SVV activates an innate immune response remain unknown. Mitofusin-2 (MFN2), a mitochondria-shaping protein regulating mitochondrial fusion and fission, plays a crucial role in innate immune responses. But, the roles of Mfn2 in SVV infection have not been elucidated. Here, we show that SVV inhibited Mfn2 expression and NLRP3 inflammasome, activating RIG-I/IRF7 signaling pathway to increase IFN-λ3 expression. Overexpression of Mfn2 inhibited RIG-I/IRF7 signaling pathway, thus decreasing IFN-λ3 expression and promoting SVV replication. Interestingly, overexpression of Mfn2 also activated NLRP3 inflammasome but did not inhibit SVV proliferation. That may mean the RIG-I/IRF7 signaling pathway plays a more important role in SVV proliferation in PK-15 cells. This study could provide important insights into the modulation of host metabolism during SVV infection and provide a strong theoretical basis for a better understanding of the pathogenic mechanism and immune activation mechanism of SVV.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2022.955671DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359100PMC
August 2022

Linkage Mapping Reveals QTL for Flowering Time-Related Traits under Multiple Abiotic Stress Conditions in Maize.

Int J Mol Sci 2022 Jul 29;23(15). Epub 2022 Jul 29.

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Variation in flowering plays a major role in maize photoperiod adaptation during long-term domestication. It is of high value to investigate the genetic basis of maize flowering under a wide range of environmental conditions in order to overcome photoperiod sensitivity or enhance stress tolerance. A recombinant inbred line (RIL) population derived from a cross between Huangzaosi and Mo17, composed of 121 lines and genotyped by 8329 specifically developed markers, was field evaluated in two consecutive years under two planting densities (67,500 and 120,000 plants ha) and two water treatments (normal irrigation and drought stress at the flowering stage). The days to silking (DTS), days to anthesis (DTA), and anthesis to silking interval (ASI) were all evaluated. Within the RIL population, DTS and DTA expanded as planting density and water deficit increased. For DTA, DTS, ASI, and ASI-delay, a total of 22, 17, 21, and 11 QTLs were identified, respectively. More than two significant QTLs were identified in each of the nine chromosomal intervals. Under diverse conditions and locations, six QTLs (quantitative trait locus) for DTS and DTA were discovered in Chr. 8: 118.13-125.31 Mb. Three chromosome regions, Chr. 3: 196.14-199.89 Mb, Chr. 8: 169.02-172.46 Mb, and Chr. 9: 128.12-137.26 Mb, all had QTLs for ASI-delay under normal and stress conditions, suggesting their possible roles in stress tolerance enhancement. These QTL hotspots will promote early-maturing or multiple abiotic stress-tolerant maize breeding, as well as shed light on the development of maize varieties with a broad range of adaptations.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/ijms23158410DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368988PMC
July 2022

Effect of methylenetetrahydrofolate reductase C677T polymorphism on serum folate but not vitamin B12 levels in patients with H-type hypertension.

Mol Biol Rep 2022 Aug 11. Epub 2022 Aug 11.

Department of Pharmacy, Xinjiang Medical University Affiliated First Hospital, Ürümqi, 830011, China.

Background: Hyperhomocysteinemia (HHcy) is a common complication in Chinese hypertensive patients and associated with methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism, folate, and vitamin B12 (Vit B12) status. This study evaluated the associations of MTHFR C677T polymorphism, folate, and Vit B12 with H-type hypertension.

Methods And Results: 887 eligible patients with essential hypertension were included. Patients were divided into two groups according to the Hcy level, the H-type hypertension group and the normal hypertension group. Related risk factors such as MTHFR polymorphism, folate and Vit B12 status were analyzed in the two groups. Age, gender, SBP, DBP, MTHFR C677T genotype, folate and Vit B12 differed significantly between H-type hypertension and normal hypertension groups (P < 0.05). MTHFR 677TT variant, gender, folate, and Vit B12 were independent risk factors for the occurrence of H-type hypertension. The risk for TT carriers was 8 times higher than that of CC and CT carriers [OR (95% CI) 8.248 (5.274-12.899)]. Male patients had almost fivefold higher odds than female patients [OR (95% CI) 4.923 (2.741-8.842)]. Folate level of patients with H-type hypertension decreased with the C to T substitution of MTFHR C677T gene (P < 0.05), while Vit B12 level was not associated with the gene (P > 0.05).

Conclusions: MTHFR 677TT variant, gender, folate, and Vit B12 were risk factors for the occurrence of H-type hypertension. Folate but not Vit B12 was associated with MTFHR C677T polymorphism in patients with H-type hypertension. Accordingly, the above factors may be considered in the prevention and treatment of hypertension.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11033-022-07844-wDOI Listing
August 2022

Deciphering the Synergies of Reductive Soil Disinfestation Combined with Biochar and Antagonistic Microbial Inoculation in Cucumber Fusarium Wilt Suppression Through Rhizosphere Microbiota Structure.

Microb Ecol 2022 Aug 11. Epub 2022 Aug 11.

School of Geography, Nanjing Normal University, Nanjing, 210023, China.

Application of reductive soil disinfestation (RSD), biochar, and antagonistic microbes have become increasingly popular strategies in a microbiome-based approach to control soil-borne diseases. The combined effect of these remediation methods on the suppression of cucumber Fusarium wilt associated with microbiota reconstruction, however, is still unknown. In this study, we applied RSD treatment together with biochar and microbial application of Trichoderma and Bacillus spp. in Fusarium-diseased cucumbers to investigate their effects on wilt suppression, soil chemical changes, microbial abundances, and the rhizosphere communities. The results showed that initial RSD treatment followed by biochar amendment (RSD-BC) and combined applications of microbial inoculation and biochar (RSD-SQR-T37-BC) decreased nitrate concentration and raised soil pH, soil organic carbon (SOC), and ammonium in the treated soils. Under RSD, the applications of Bacillus (RSD-SQR), Trichoderma (RSD-T37), and biochar (RSD-BC) suppressed wilt incidence by 26.8%, 37.5%, and 32.5%, respectively, compared to non-RSD treatments. Moreover, RSD-SQR-T37-BC and RSD-T37 caused greater suppressiveness of Fusarium wilt and F. oxysporum by 57.0 and 33.5%, respectively. Rhizosphere beta diversity and alpha diversity revealed a difference between RSD-treated and non-RSD microbial groups. The significant increase in the abundance, richness, and diversity of bacteria, and the decrease in the abundance and diversity of fungi under RSD-induced treatments attributed to the general suppression. Identified bacterial (Bacillus, Pseudoxanthomonas, Flavobacterium, Flavisolibacter, and Arthrobacter) and fungal (Trichoderma, Chaetomium, Cladosporium, Psathyrella, and Westerdykella) genera were likely the potential antagonists of specific disease suppression for their significant increase of abundances under RSD-treated soils and high relative importance in linear models. This study infers that the RSD treatment induces potential synergies with biochar amendment and microbial applications, resulting in enhanced general-to-specific suppression mechanisms by changing the microbial community composition in the cucumber rhizosphere.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00248-022-02097-3DOI Listing
August 2022

First Report of Tobacco Root Rot Caused by in China.

Plant Dis 2022 Aug 8. Epub 2022 Aug 8.

Xuchang, China;

China is the largest producer of tobacco (Nicotiana tabacum L.) in the world with an estimated production of 2.4 million ton per year (Berbeć and Matyka 2020). In June 2021, a root disease was observed on tobacco in three surveyed counties (Xiangcheng, Linying and Jiaxian) in central Henan. Diseased plants exhibited leaf chlorosis and brown to purplish vascular discoloration of the taproot and lateral roots. Approximately 10 to 15% of the plants were symptomatic in the nine fields surveyed, representing 60 ha in total. Root segments (0.5 to 1 cm) from ten diseased plants were surface sterilized in 75% ethanol for 30 s followed by rinsing with sterile distilled water three times. Thirty air dried root pieces were placed on potato dextrose agar (PDA) and incubated at 25℃ in the dark for 2 days. Typical spp. colonies were obtained from all root samples. Ten pure cultures were obtained by single-spore culturing (Yz01 to Yz10). Colonies on PDA showed abundant white to cream aerial mycelia with a yellowish-brown center on the reverse side after 7 days, and an average growth rate of 5 mm/day. From 7-day-old cultures grown on carnation leaf agar (CLA), macroconidia had three to four septa, were falciform, with blunt apical cells and slightly hooked basal cell, and measured 20 to 41×3-6.5 μm (n=50). Spherical conidia clusters were formed at the apex of the conidiophores. Abundant reniform and cylindrical microconidia were one to two-celled, with apexes rounded, measuring 7 to 15×2 to 5 μm (n=50). The roughly spherical chlamydospores were intercalary or terminal, single or in chains, and rough walled. Such characteristics were consistent with the species complex (FSSC) (Leslie and Summerell 2006). The translation elongation factor 1-alpha () gene of the ten cultures was amplified with primers (O'Donnell et al. 1998), and sequenced. Maximum likelihood analysis was carried out using the sequences of the ten cultures (Kumar et al. 2016). The RNA polymerase I largest subunit () and second largest subunit () genes of the cultures were amplified with primers and respectively (O'Donnell et al. 1998, 2010), and sequenced. The , and sequences (GenBank accession nos. ON186742.1-ON186751.1, ON241133.1-ON241148.1, ON324054.1-ON324057.1) were 99.4 to 100% identical to the corresponding DNA sequences of based on FUSARIUM-ID BLASTn analysis. Morphological and molecular results confirmed this species as (Díaz-Nájera et al. 2021; Velarde-Félix et al. 2022). Pathogenicity tests were performed in tobacco seedlings grown on autoclaved soil. Healthy six-leaf stage tobacco seedlings (n=30; Zhongyan 100) were inoculated by placing 7-days old wheat seed (15 seeds per plant) infested with the representative culture Yz07 around the root. Thirty seedlings inoculated with sterile wheat seeds served as controls. All the plants were maintained in a growth chamber at 25±0.5℃ and 70% relative humidity. The assay was conducted three times. Typical symptoms of foliage chlorosis and root browning were observed 7 to 14 days after inoculation for all the 90 inoculated seedlings. Fifteen diseased seedlings were randomly selected for tissue isolation, and was reisolated from the 15 seedlings and showed the same morphology and gene sequence as the original isolate. Control plants remained asymptomatic and no pathogen was recovered. The results showed that can cause root rot of tobacco. was reported to cause tobacco wilt and root rot in Northwestern Argentina (Berruezo et al. 2018); however, this is the first report of causing root rot of tobacco in China. This species was previously reported in China affecting (Shen et al. 2019) and (Zhang et al. 2020), showing that has a broad host range in this country. These results may inform control tobacco root rot through improve crop rotations. Funding: Funding was provided by the Science and Technology Project of Henan Provincial Tobacco Company (2020410000270012), Outstanding Youth Science and Technology Fund Project of Henan Academy of Agricultural Sciences (2022YQ09) and Science and Technology Innovation Team project of Henan Academy of Agricultural Sciences (2022TD26). References: Berbeć, A. K., and Matyka, M. 2020. Agric. 10:551. Berruezo, L. A., et al. 2018. Eur. J. Plant. Pathol. 151:1065. Díaz-Nájeraet, J. F., et al. 2021. Plant Dis. 105:710. Douriet-Angulo, A., et al. 2019. Plant Dis. 103:11. Kumar, S., et al. 2016. Mol. Biol. Evol. 33:1870. Leslie, J. F., and Summerell, B. A., eds. 2006. The Fusarium Laboratory Manual. Blackwell Publishing, Ames, IA. O'Donnell, K., et al. 1998. PNAS. 95:2044. O'Donnell, K., et al. 2010. J. Clin. Microbiol. 48:3708. Vega-Gutierrez, T. A., et al. 2018. Plant Dis. 103:1. Velarde-Félix, S., et al. 2022. Plant Dis. 106:329. Zhang, X., et al. 2020. Plant Dis. 104:5. The author(s) declare no conflict of interest. : tobacco root rot, , China.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1094/PDIS-06-22-1394-PDNDOI Listing
August 2022

Systemic colonization of potato plants by Verticillium dahliae leads to infection of tubers and sprouting buds.

Plant Dis 2022 Aug 8. Epub 2022 Aug 8.

College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, hohhot, Inner Mongolia, China;

A green fluorescent protein (GFP)-tagged isolate of Verticillium dahliae was used to study its colonization in potato plants and tubers. Three-week-old potato plants of the highly susceptible cultivar 'Shepody' were inoculated with a conidial suspension of a GFP-tagged isolate of V. dahliae using a wound inoculation method. Colonization was studied using confocal microscopy combined with tissue sections. Conidia germinated and hyphae grew along the root hairs, elongation zones, and root caps between 24 to 96 hours post-inoculation (HPI). At seven days post-inoculation (DPI), the pathogen advanced to cortical tissues and grew into the root vascular bundles. At eight weeks post-inoculation (WPI), the stem epidermal cells, cortical tissues, vascular elements, and petioles were fully colonized by the mycelium of V. dahliae. At 11 WPI, the pathogen was detected in the stolon and progeny tubers, as confirmed by both GFP signals in tissues and re-isolation of the pathogen on the semi-selective NP-10 medium. Progeny potato tubers were harvested from the inoculated potato plants, and the GFP-signal was observed in the epidermal cells and vascular elements of sprouting buds that emerged from the harvested tubers. The infection rate of progeny tubers detected on semi-selective NP-10 medium ranged from 34.55% to 55.56%, with an average of 45.31%. In conclusion, we report, for the first time, the entire progression of colonization by V. dahliae of potato plant tissues, progeny tubers, as well as of the sprouting buds that emerged from progeny tubers.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1094/PDIS-05-22-1029-REDOI Listing
August 2022

microRNA-497-mediated Smurf2/YY1/HIF2α axis in tumor growth and metastasis of esophageal squamous cell carcinoma.

J Biochem Mol Toxicol 2022 Aug 8:e23182. Epub 2022 Aug 8.

Department of Thoracic Surgery, First Affiliated Hospital of Soochow University, Suzhou, China.

Aberrant expression of microRNA-497 (miR-497) is associated with tumor progression, but the molecular mechanisms in tumorigenesis remain largely unknown. Here, we report that miR-497 expression is downregulated in esophageal squamous cell carcinoma (ESCC) clinical samples. Consistently, upregulation of miR-497 inhibits ESCC cell malignant properties and tumor growth in vivo. Importantly, we uncovered that miR-497 upregulation suppressed ESCC cell growth and tumor growth by inhibiting Smurf2. Mechanistically, we showed that Smurf2 was a target of miR-497, and mediated YY1 expression to elevate HIF2α expression, thereby enhancing the malignancy of ESCC cells. Together, our study uncovered the role of the miR-497-mediated Smurf2/YY1/HIF2α axis in tumor growth and metastasis, which might provide potential therapeutic targets for human ESCC.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/jbt.23182DOI Listing
August 2022

TiO NPs/h-BN: Preparation and catalytic activities of a novel AP catalyst.

Front Chem 2022 22;10:947052. Epub 2022 Jul 22.

West Anhui University, Lu'an, China.

The thermal decomposition performance of an oxidizer directly determines the thrust and specific impulse properties of the solid propellant. Hexagonal boron nitride (h-BN) has the characteristics of high catalytic activity and good stability, which can improve the heat release and decomposition temperature of the oxidant, and then improve the energy performance of the propellant. In this study, a novel hybrid material TiO NPs/h-BN was successfully prepared by growth, and it was found that when 5 wt.% TiO NPs/h-BN was added, the initial decomposition temperature of ammonium perchlorate (AP) decreased by 67.6°C. Due to the addition of TiO, the gap between the h-BN layers as well as the specific surface increased, which optimized its thermocatalytic performance, and it also proposed a catalytic mechanism for the thermal decomposition process of AP.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fchem.2022.947052DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354831PMC
July 2022

Self-Powered, Long-Durable, and Highly Selective Oil-Solid Triboelectric Nanogenerator for Energy Harvesting and Intelligent Monitoring.

Nanomicro Lett 2022 Aug 5;14(1):160. Epub 2022 Aug 5.

Division of Machine Elements, Department of Engineering Sciences and Mathematics, Luleå University of Technology, 971 87, Luleå, Sweden.

Triboelectric nanogenerators (TENGs) have potential to achieve energy harvesting and condition monitoring of oils, the "lifeblood" of industry. However, oil absorption on the solid surfaces is a great challenge for oil-solid TENG (O-TENG). Here, oleophobic/superamphiphobic O-TENGs are achieved via engineering of solid surface wetting properties. The designed O-TENG can generate an excellent electricity (with a charge density of 9.1 µC m and a power density of 1.23 mW m), which is an order of magnitude higher than other O-TENGs made from polytetrafluoroethylene and polyimide. It also has a significant durability (30,000 cycles) and can power a digital thermometer for self-powered sensor applications. Further, a superhigh-sensitivity O-TENG monitoring system is successfully developed for real-time detecting particle/water contaminants in oils. The O-TENG can detect particle contaminants at least down to 0.01 wt% and water contaminants down to 100 ppm, which are much better than previous online monitoring methods (particle > 0.1 wt%; water > 1000 ppm). More interesting, the developed O-TENG can also distinguish water from other contaminants, which means the developed O-TENG has a highly water-selective performance. This work provides an ideal strategy for enhancing the output and durability of TENGs for oil-solid contact and opens new intelligent pathways for oil-solid energy harvesting and oil condition monitoring.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s40820-022-00903-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356124PMC
August 2022

A simple and practical intraoperative ventilation technique for uniportal video-assisted thoracoscopic tracheal reconstruction: a case report.

Ann Transl Med 2022 May;10(9):529

Department of Thoracic Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Cross-field endotracheal intubation is typically performed during tracheal anastomosis to maintain single-lung ventilation. To minimize obstruction of the surgical field by the cross-field tube, special equipment such as high-frequency jet ventilation (HFJV) and extracorporeal membrane oxygenation (ECMO) or advanced techniques such as non-intubated ventilation have been proposed. Here, we describe a simple and practical airway management strategy that requires only conventional ventilators and techniques. Our operation is completed under uniportal video-assisted thoracoscopic surgery (VATS).

Case Description: We report a case of tracheal adenoid cystic carcinoma (ACC) presenting with cough with bloody sputum in a 53-year-old man. Computed tomography (CT) and flexible bronchoscopy revealed an irregular polypoid neoplasm attached to the right wall of the distal trachea, which almost completely blocked the tracheal lumen. To relieve the symptoms, transbronchoscopic resection of the tumor, followed by curative resection via uniportal VATS under general anesthesia was performed. To maintain single-lung ventilation during tracheal reconstruction, we took advantage of a thin suction tube [internal diameter (ID) 3 mm; external diameter (ED) 4 mm], which was connected to a conventional ventilator. Specifically, by introducing the suction tube into the distal left main bronchus through the endotracheal tube and blowing 100% oxygen, we achieved satisfactory oxygenation throughout the anastomotic process; and the blood CO partial pressure was also acceptable. The view of the anastomotic site was far less obstructed owing to the small diameter of the suction tube, and the anastomotic process was smooth and accurate. Postoperative recovery was good, and no stenosis of the reconstructed trachea was observed at the 3-month follow-up.

Conclusions: Our technique proves to be safe and feasible for selected patients with tracheal tumors, and can be a practical choice for medical centers that are not equipped with HFJV or ECMO.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.21037/atm-21-6215DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347034PMC
May 2022

Exploring the Relationship Between Binocular Imbalance and Myopia: Refraction with a Virtual Reality Platform.

Cyberpsychol Behav Soc Netw 2022 Aug 1. Epub 2022 Aug 1.

Department of Strabismus & Pediatric Ophthalmology, Shenzhen Eye Hospital Affiliated to Jinan University, Shenzhen University of Medicine, Shenzhen, China.

To explore the relationship between binocular imbalance (BI) and the abnormal development of binocular refraction. BI data were collected by enrolling the first 1,000 adolescents and children aged 6-18 years in Shenzhen Eye Hospital from April 2020 to January 2021. In this cross-sectional study, the imbalance value (IV) did not show a statistical correlation with the spherical equivalent (SE) (oculus dexter [OD]:  = 0.022,  = 0.586; oculus sinister [OS]:  = -0.021,  = 0.606), and had little correlation with the uncorrected visual acuity (VA) (OD:  = -0.084,  = 0.039; OS:  = -0.034,  = 0.408). The proportion of binocular contrast imbalance (BCI) (the absolute value) maintained the highest level (from 54.42 to 79.17 percent) with the increase of bilateral SE difference in the four subcategories (binocular balance, monocular suppression, binocular rivalry, and BCI). From -100 to +100 of IV, the SE of the left eye tends to increase negatively when compared with the right eye (from -95 < IV ≦ -80, SE difference = -0.83 ± 1.58, to -20 < IV ≦ -10, SE difference = -0.14 ± 0.61; from 10 ≦ IV <20, SE difference = -0.05 ± 0.80, to 80 ≦ IV <95, SE difference = 1.48 ± 2.77). BI widely exists within the general pediatric population. The BI did not show significant correlation with the unilateral eye refractive state and the VA. However, the BI may be accompanied by imbalanced development of the eye refractive system. Furthermore, the SE of the dominant eye (from the prospective of BI) tends to be more negative than that of the opposite eye as the value increases. Clinical Trial Registration number: ChiCTR2100045457.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1089/cyber.2022.0162DOI Listing
August 2022

Chloroplastic photoprotective strategies differ between bundle sheath and mesophyll cells in maize ( L.) Under drought.

Front Plant Sci 2022 14;13:885781. Epub 2022 Jul 14.

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.

Bundle sheath cells play a crucial role in photosynthesis in C4 plants, but the structure and function of photosystem II (PSII) in these cells is still controversial. Photoprotective roles of bundle sheath chloroplasts at the occurrence of environmental stresses have not been investigated so far. Non-photochemical quenching (NPQ) of chlorophyll a fluorescence is the photoprotective mechanism that responds to a changing energy balance in chloroplasts. In the present study, we found a much higher NPQ in bundle sheath chloroplasts than in mesophyll chloroplasts under a drought stress. This change was accompanied by a more rapid dephosphorylation of light-harvesting complex II (LHCII) subunits and a greater increase in PSII subunit S (PsbS) protein abundance than in mesophyll cell chloroplasts. Histochemical staining of reactive oxygen species (ROS) suggested that the high NPQ may be one of the main reasons for the lower accumulation of ROS in bundle sheath chloroplasts. This may maintain the stable functioning of bundle sheath cells under drought condition. These results indicate that the superior capacity for dissipation of excitation energy in bundle sheath chloroplasts may be an environmental adaptation unique to C4 plants.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2022.885781DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330506PMC
July 2022

The association between the pre-pregnancy vaginal microbiome and time-to-pregnancy: a Chinese pregnancy-planning cohort study.

BMC Med 2022 08 1;20(1):246. Epub 2022 Aug 1.

Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, No. 87 Dingjiaqiao Rd., Gulou District, Nanjing, Jiangsu, China.

Background: Although sexually transmitted infections are regarded as the main cause of tubal infertility, the association between the common vaginal microbiome and female fecundability has yet to be determined. The objective of this study was to find convincing evidence relating to the impact of the vaginal bacterial structure on the fecundability of women planning pregnancy.

Methods: We recruited women who took part in the Free Pre-pregnancy Health Examination Project from 13 June 2018 to 31 October 2018 (n = 89, phase I) and from 1 November 2018 to 30 May 2020 (n = 389, phase II). We collected pre-pregnancy vaginal swabs from each subject; then, we followed up each subject to acquire the pregnancy-planning outcome in 1 year. In phase I, 16S rRNA gene sequencing was performed to investigate the vaginal bacterial content between the pregnancy and non-pregnancy groups. These findings were verified in phase II by applying a quantitative real-time polymerase chain reaction for the measurement of the absolute abundance of specific species. Cox models were used to estimate fecundability ratios (FR) for each vaginal microbiome type.

Results: In phase I, 59.6% (53/89) of women became pregnant within 1 year. The principal coordinate analysis showed that the pre-pregnancy vaginal microbial community structures of the pregnant and non-pregnant groups were significantly different (PERMANOVA test, R = 0.025, P = 0.049). The abundance of the genus Lactobacillus in the pregnancy group was higher than that of the non-pregnant group (linear discriminant analysis effect size (LDA) > 4.0). The abundance of the genus Gardnerella in the non-pregnant group was higher than those in the pregnant group (LDA > 4.0). In phase II, female fecundability increased with higher absolute loads of Lactobacillus gasseri (quartile Q4 vs Q1, FR = 1.71, 95%CI 1.02-2.87) but decreased with higher absolute loads of Fannyhessea vaginae (Q4 vs Q1, FR = 0.62, 95%CI 0.38-1.00). Clustering analysis showed that the vaginal microbiome of type D (characterized by a higher abundance of Lactobacillus iners, a lower abundance of Lactobacillus crispatus and Lactobacillus gassri) was associated with a 55% reduction of fecundability (FR = 0.45, 95%CI 0.26-0.76) compared with type A (featuring three Lactobacillus species, low Gardnerella vaginalis and Fannyhessea vaginae abundance).

Conclusions: This cohort study demonstrated an association between the pre-pregnancy vaginal microbiome and female fecundability. A vaginal microbiome characterized by a higher abundance of L. iners and lower abundances of L. crispatus and L. gasseri appeared to be associated with a lower fecundability. Further research now needs to confirm whether manipulation of the vaginal microenvironment might improve human fecundability.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12916-022-02437-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9341075PMC
August 2022

Paranasal Augmentation: A Viable and Simplified Modality With Diced Expanded Polytetrafluoroethylene.

J Craniofac Surg 2022 Jul 28. Epub 2022 Jul 28.

Department No. 16 of Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing.

A flat or concave lower midface profile is generally considered less attractive. Paranasal augmentation is usually performed to move paranasal deficiency to relative convexity. Herein, we present a viable and simplified modality with diced expanded polytetrafluoroethylene to correct paranasal deficiency. Between February of 2020 and April of 2021, 19 patients underwent procedures to correct paranasal deficiency. Paranasal augmentation was performed with diced expanded polytetrafluoroethylene. All procedures were performed via intranasal approach. Preoperative and postoperative photographs were taken. Of these 19 patients (18 women and 1 man), deficiency of 18 patients were caused by congenital factors and 1 by cleft deformities. All but 1 procedures were carried out bilaterally. Procedures were performed in conjunction with additional operations mainly including rhinoplasty and minimally invasive midface lift. Age of patients ranged from 19 to 57 years, with a mean of 37 years. Follow-up time ranged from 8 to 22 months, with a mean of 12.6 months. All patients were satisfied with esthetic improvement and facial holistic profile. No severe complications and reoperation arisen in any of the patients. Temporary discomfort involved foreign body sensation in 1 case and numbness in 2 cases. The present technique provides a viable and simplified method to give the face a more balanced appearance and achieves esthetically superior results.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/SCS.0000000000008676DOI Listing
July 2022

Comparison of HPV neutralizing and IgG antibodies in unvaccinated female adolescents.

Future Microbiol 2022 Jul 29. Epub 2022 Jul 29.

State Key Laboratory of Molecular Vaccinology & Molecular Diagnostics, National Institute of Diagnostics & Vaccine Development in Infectious Diseases, Strait Collaborative Innovation Center of Biomedicine and Pharmaceutics, School of Public Health, Xiamen University, Xiamen, 361102, Fujian, China.

To analyze the consistency between HPV neutralizing antibodies and specific total IgG antibodies in unvaccinated females. Serum samples from 978 unvaccinated Chinese females aged 9-26 years were measured for antibodies against HPV-16 and HPV-18 using simultaneous pseudovirus-based neutralization assay and ELISA. There was a moderate level of consistency between HPV neutralizing antibodies and specific IgG in females aged 18-26 years (Cohen's κ coefficient for HPV-16 and HPV-18: 0.52 and 0.38) and poor consistency in those aged 9-17 years (Cohen's κ coefficient <0.05). However, Cohen's κ coefficient remained almost unchanged in sensitivity analysis when the IgG antibody cut-off value was raised. HPV neutralizing antibodies are a more specific indicator for the evaluation of HPV natural humoral immunity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2217/fmb-2021-0221DOI Listing
July 2022

Identification and comparison of biological characteristics and pathogenicity of different mating types of V. dahliae isolated from potato and sunflower.

Sci Rep 2022 Jul 27;12(1):12840. Epub 2022 Jul 27.

College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, 010018, China.

Potato is one of the most important staple crops in the world. China is one of the leading producers of potatoes, but the industry faces soilborne diseases such as Verticillium wilt. Most potato planting areas in China rotate the crop with sunflower which is also highly susceptible to Verticillium wilt. The comparison of the biological characteristics and pathogenicity of different mating types of Verticillium dahliae isolated from potato and sunflower in the major planting regions in China is of great importance. This is to help unravel the diversity in V. dahliae population and the sudden increase in infected fields. The diseased samples collected were cultured on PDA and the growing colony of pathogen isolated. Molecular techniques using specific primers were used to identify the V. dahliae pathogens and their mating type of the isolates obtained from the diseased sunflower and potato plants as well as their planting materials. The data obtained revealed that the dominant mating type population in sunflower was MAT1-1, whiles that of potato was MAT1-2, but Race 2 was the only race type identified for all the samples. There was a significant presence of MAT1-1 isolates present in potatoes, which is a new trend. Conventional crop rotation farming using sunflower is causing an increasing prevalence of MAT1-1 and mating type shift of isolates in potato in these regions.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-022-17196-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9329468PMC
July 2022

Synergistic Covalent-and-Supramolecular Polymers with an Interwoven Topology.

ACS Appl Mater Interfaces 2022 Jul 27. Epub 2022 Jul 27.

School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules, Shanghai Key Laboratory of Electrical Insulation and Thermal Aging, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.

Network topologies, especially some high-order topologies, are able to furnish cross-linked polymer materials with enhanced properties without altering their chemical composition. However, the fabrication of such topologically intriguing architectures at the macromolecular level and in-depth insights into their structure-property relationship remain a significant challenge. Herein, we relied on synergistic covalent-and-supramolecular polymers (CSPs) as a platform to prepare a range of polymer networks with an interwoven topology. Specifically, through the sequential supramolecular self-assemblies, the covalent polymers (CPs) and metallosupramolecular polymers (MSPs) could be interwoven in our CSPs by [2]pseudorotaxane cross-links. As a result, the obtained CSPs possessed a topological network that could not only promote the synergistic effect between CPs and MSPs to afford mechanically robust yet dynamic materials but also vest polymers with some functions, as manifested by force-induced hierarchical dissociations of supramolecular interactions and superior thermomechanical stability compared to our previously reported CSP systems. Furthermore, our CSPs exhibited tunable mechanical performance toward multiple stimuli including K and PPh, demonstrating abundant stimuli-responsive properties. We hope that these findings could provide novel opportunities toward achieving topological structures at the macromolecular level and also motivate further explorations of polymeric materials via the way of controlling their topological structures.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.2c10404DOI Listing
July 2022

LINC00665/miRNAs axis-mediated collagen type XI alpha 1 correlates with immune infiltration and malignant phenotypes in lung adenocarcinoma.

Open Med (Wars) 2022 13;17(1):1259-1274. Epub 2022 Jul 13.

Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Medical College of Soochow University, No. 899 Pinghai Road, Gusu District, Suzhou 215006, Jiangsu Province, China.

Collagen type XI alpha 1 (COL11A1) as an oncogene has been reported in several malignant tumors. Herein, we aimed to explore the function of COL11A1 and its upstream regulators in lung adenocarcinoma (LUAD). COL11A1 expression prognostic significance, gene ontology, Kyoto Encyclopedia of Genes and Genomes, and immune infiltration were explored in LUAD. experimental measurements were implemented to validate the function of COL11A1 and LINC00665 in LUAD cells. Our study demonstrated that LINC00665-2 and COL11A1 were significantly upregulated in LUAD tissues compared with nontumor tissues. COL11A1 was positively correlated with multiple immune cell enrichment, suggesting that COL11A1 may be a prospective therapeutic target to enhance the efficacy of immunotherapy in LUAD. A regulatory mechanism LINC00665-2/microRNAs (miRNAs)/COL11A1 axis was identified to facilitate the tumorigenesis of LUAD. si-LINC00665 transfection induced the inhibition of growth and migration, and apoptosis was reversed by the overexpression of COL11A1 in LUAD cells. In conclusion, LINC00665 as a competing endogenous RNA sponging multiple miRNAs to modulate COL11A1 expression in LUAD, suggesting that LINC00665/miRNAs/COL11A1 axis may contribute to the pathogenesis of LUAD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1515/med-2022-0478DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9281593PMC
July 2022

Study on Static and Fatigue Behaviors of Steel-UHPFRC Composite Deck Structure.

Polymers (Basel) 2022 Jul 8;14(14). Epub 2022 Jul 8.

Yellow River Engineering Consulting Co., Ltd., Zhengzhou 450003, China.

Ultra-high-performance fiber-reinforced cementitious composite (UHPFRC) is used in orthotropic steel deck (OSD) to form a lightweight composite deck structure (LWCD), which is expected to solve the problems of fatigue cracking of traditional steel deck and pavement damage. This paper aims to study the influence of key design parameters on longitudinal bending and transverse fatigue performance, as well as the ultimate bearing capacity calculation theory of the LWCD. A local finite-element (FE) model was built to evaluate the vehicle-induced stress ranges of six typical fatigue-prone details. In total, eight negative bending tests on steel-UHPFRC composite beams and one fatigue test on a steel-UHPFRC composite plate were conducted to investigate the longitudinal bending performance and the transverse flexural fatigue behavior of the LWCD, respectively. The results show that adding a 60-mm UHPFRC layer can significantly reduce the stress amplitude of six typical fatigue details by 44.8% to 90%. The failure mode of the longitudinal bending tests is the U-rib buckle and all UHPFRC layers exhibit multiple cracking behaviors when the specimens failed. The longitudinal cracking stresses of the specimens are between 20.0 MPa to 27.3 MPa. The reinforcement ratio and cover thickness have a great influence on the cracking stress. While the ultimate bearing capacity of specimens with different parameters has little difference. The calculation method of the ultimate bearing capacity of a steel-UHPFRC composite structure is proposed. When the strain at the bottom of the u-rib is taken as 1.2 times the design yield strain, the calculated results are in good agreement with the experimental results. No fatigue failure was observed after 66.12 million fatigue cycles under the design load, highlighting the favorable fatigue resistance of the proposed LWCD.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/polym14142796DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9317259PMC
July 2022

Antiviral Activity of Porcine IFN-λ3 and IFN-α against Porcine Rotavirus In Vitro.

Molecules 2022 Jul 18;27(14). Epub 2022 Jul 18.

College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 625014, China.

Interferons (IFNs) play a major role in the host's antiviral innate immunity. In response to viral infection, IFNs bind their receptors and initiate a signaling cascade, leading to the accurate transcriptional regulation of hundreds of IFN-stimulated genes (ISGs). Porcine rotavirus (PoRV) belongs to genus of the family; the infection is a global epidemic disease and a major threat to the pig industry. In this study, we found that IFN-λ3 inhibited the replication of PoRV in both MA104 cells and IPEC-J2 cells, and this inhibition was dose-dependent. Furthermore, the antiviral activity of IFN-λ3 was more potent in IPEC-J2 cells than in MA104 cells. Further research showed that IFN-λ3 and IFN-α might inhibit PoRV infection by activating ISGs, i.e., MxA, OASL and ISG15, in IPEC-J2 cells. However, the co-treatment of IFN-λ3 and IFN-α did not enhance the antiviral activity. Our data demonstrated that IFN-λ3 had antiviral activity against PoRV and may serve as a useful antiviral candidate against PoRV, as well as other viruses in swine.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules27144575DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321941PMC
July 2022

Impact of total solids content on biochar amended co-digestion of food waste and sludge: Microbial community dynamics, methane production and digestate quality assessment.

Bioresour Technol 2022 Jul 23;361:127682. Epub 2022 Jul 23.

Institute of Bioresource and Agriculture, Sino-Forest Applied Research Centre for Pearl River Delta Environment, Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong SAR, China. Electronic address:

This study evaluates the impact of biochar addition on the performance of anaerobic co-digestion of food waste (FW) and sewage sludge at different total solids (TS) contents (2.5 %, 5.0 %, and 7.5 %). Biochar co-digestion improved hydrolysis and acidogenesis by neutralizing volatile fatty acids (VFAs) reducing its inhibitions (2.6-fold removal), which elevated the soluble chemical oxygen demand (sCOD) degradation by 2.5 folds leading to a higher cumulative methane production compared to the control. This increase corresponded to an improvement of methane yields by ∼21 %-33 % (242-340 mL/gVS) at different TS contents. The biochar surface area offered substantial support for direct interspecies electron transfer (DIET) activity, and biofilm-mediated growth of methanogens i.e., Methanosarcina, Methanosata, and Methanobrevibacter. The biochar-enriched digestate improved the seed germination index, and bioavailability of plant nutrients such as N, P, K, and NHN. This study reports an improved biochar-mediated anaerobic co-digestion for efficient and sustainable FW valorization.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2022.127682DOI Listing
July 2022

Cometabolic biodegradation system employed subculturing photosynthetic bacteria: A new degradation pathway of 4-chlorophenol in hypersaline wastewater.

Bioresour Technol 2022 Jul 22;361:127670. Epub 2022 Jul 22.

College of Environment, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, Zhejiang 310014, PR China. Electronic address:

4-chlorophenol (4-CP) as a toxic persistent pollutant is quite difficult treatment by using traditional biological processes. Herein, photosynthetic bacteria (PSB) driven cometabolic biodegradation system associated with exogeneous carbon sources (e.g., sodium acetate) has been demonstrated as an effective microbial technique. The biodegradation rate (r) can be at 0.041 d with degradation efficiency of 93% in 3094 lx. Through the study of subculturing PSB in absence of NaCl, it was found that 50% inoculation time can be saved but keeping a similar 4-CP biodegradation efficiency in scale-up salinity system. A new plausible biodegradation pathway for 4-CP in 4th G PSB cometabolic system is proposed based on the detected cyclohexanone generation followed by ring opening. It is probably ascribed to the increasement of Firmicutes and Bacteroidetes at phyla level classified based on microbial community. This study contributes to a new insight into cometabolic technology for chlorophenol treatment in industrial hypersaline wastewater.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biortech.2022.127670DOI Listing
July 2022

Plant regeneration via callus-mediated organogenesis in commercial variety of Chuanbeichai No. 1 in DC.

Plant Biotechnol Rep 2022 Jul 16:1-11. Epub 2022 Jul 16.

School of Life Science and Engineering, Southwest University of Science and Technology, 59 Qinglong Road, Mianyang, 621010 Sichuan China.

DC is an important medicinal plant with many active ingredients that are used for the treatment of different types of diseases and valued in pharmaceutical markets. In vitro shoot regeneration can efficiently contribute to the improvement of In the present study, we investigated the effects of the explant type and plant growth regulators (PGRs) on embryogenic callus induction and plant regeneration in . Our investigation demonstrated that 2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) combined with 1 mg/L thidiazuron (TDZ) played a major role in promoting callus induction from leaf, hypocotyl and stem 2 explants, whereas the most effective treatment for stem 1 callus formation was Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-D, 0.5 mg/L 6-benzyladenine (BA) and 0.5 mg/L kinetin (Kin). The highest shoot regeneration rate (57.14%) was obtained from hypocotyl-induced calli in MS medium with 0.5 mg/L Kin after 12 weeks of cultivation. This regeneration protocol can be used in large-scale cultivation and may be useful for future genetic modifications of
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11816-022-00772-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9288091PMC
July 2022

Gross tumor volume delineation in primary prostate cancer on F-PSMA-1007 PET/MRI and Ga-PSMA-11 PET/MRI.

Cancer Imaging 2022 Jul 22;22(1):36. Epub 2022 Jul 22.

Department of Radiation Oncology, Shanghai East Hospital, Tongji University School of Medicine, 200120, Shanghai, China.

Background: We aimed to assess the clinical value of F-PSMA-1007 and Ga-PSMA-11 PET/MRI in the gross tumor volume (GTV) delineation of radiotherapy for prostate cancer (PCa).

Methods: Sixty-nine patients were retrospectively enrolled (57 in the F subgroup and 12 in the Ga subgroup). Three physicians delineated the GTV and tumor length by the visual method and threshold method with thresholds of 30%, 40%, 50%, and 60% SUVmax. The volume correlation and differences in GTVs were assessed. The dice similarity coefficient (DSC) was applied to estimate the spatial overlap between GTVs. For 51 patients undergoing radical prostatectomy, the tumor length (Lpath) of the maximum area was measured, and compared with the longest tumor length obtained based on the images (L, L, L, L, L, L, L) to determine the best delineation method.

Results: In the F subgroup, (1) GTV-PET/MRI (p < 0.001) was significantly different from the reference GTV-MRI. DSC between them was > 0.7. (2) GTV-MRI (R = 0.462, p < 0.05) was the influencing factor of DSC. In the Ga subgroup, (1) GTV-PET/MRI (p < 0.05) was significantly different from the reference GTV-MRI. DSC between them was > 0.7. (2) There was a significant correlation between GTV-MRI (r = 0.580, p < 0.05) and DSC. The longest tumor length measured by PET/MRI was in good agreement with that measured by histopathological analysis in both subgroups.

Conclusion: It is feasible to visually delineate GTV on PSMA PET/MRI in PCa radiotherapy, and we emphasize the utility of PET/MRI fusion images in GTV delineation. In addition, the overlap degree was the highest between GTV-MRI and GTV-PET/MRI, and it increased with increasing volume.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s40644-022-00475-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308314PMC
July 2022
-->