Publications by authors named "Jun Zhang"

7,847 Publications

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Transformer-based unsupervised contrastive learning for histopathological image classification.

Med Image Anal 2022 Jul 30;81:102559. Epub 2022 Jul 30.

Tencent AI Lab, Shenzhen 518057, China. Electronic address:

A large-scale and well-annotated dataset is a key factor for the success of deep learning in medical image analysis. However, assembling such large annotations is very challenging, especially for histopathological images with unique characteristics (e.g., gigapixel image size, multiple cancer types, and wide staining variations). To alleviate this issue, self-supervised learning (SSL) could be a promising solution that relies only on unlabeled data to generate informative representations and generalizes well to various downstream tasks even with limited annotations. In this work, we propose a novel SSL strategy called semantically-relevant contrastive learning (SRCL), which compares relevance between instances to mine more positive pairs. Compared to the two views from an instance in traditional contrastive learning, our SRCL aligns multiple positive instances with similar visual concepts, which increases the diversity of positives and then results in more informative representations. We employ a hybrid model (CTransPath) as the backbone, which is designed by integrating a convolutional neural network (CNN) and a multi-scale Swin Transformer architecture. The CTransPath is pretrained on massively unlabeled histopathological images that could serve as a collaborative local-global feature extractor to learn universal feature representations more suitable for tasks in the histopathology image domain. The effectiveness of our SRCL-pretrained CTransPath is investigated on five types of downstream tasks (patch retrieval, patch classification, weakly-supervised whole-slide image classification, mitosis detection, and colorectal adenocarcinoma gland segmentation), covering nine public datasets. The results show that our SRCL-based visual representations not only achieve state-of-the-art performance in each dataset, but are also more robust and transferable than other SSL methods and ImageNet pretraining (both supervised and self-supervised methods). Our code and pretrained model are available at https://github.com/Xiyue-Wang/TransPath.
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http://dx.doi.org/10.1016/j.media.2022.102559DOI Listing
July 2022

An Antibody from Single Human V-rearranging Mouse Neutralizes All SARS-CoV-2 Variants Through BA.5 by Inhibiting Membrane Fusion.

Sci Immunol 2022 Aug 11:eadd5446. Epub 2022 Aug 11.

Howard Hughes Medical Institute, Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA.

SARS-CoV-2 Omicron sub-variants have generated a world-wide health crisis due to resistance to most approved SARS-CoV-2 neutralizing antibodies and evasion of vaccination-induced antibodies. To manage Omicron sub-variants and prepare for potential new variants, additional means of isolating broad and potent humanized SARS-CoV-2-neutralizing antibodies are desirable. Here, we describe a mouse model in which the primary B cell receptor (BCR) repertoire is generated solely through V(D)J recombination of a human V1-2 heavy chain (HC) and, substantially, a human Vκ1-33 light chain (LC). Thus, primary humanized BCR repertoire diversity in these mice derives from immensely diverse HC and LC antigen-contact complementarity-region-3 (CDR3) sequences generated by non-templated junctional modifications during V(D)J recombination. Immunizing the human V1-2/Vκ1-33-rearranging mouse model with SARS-CoV-2 (Wuhan-Hu-1) spike protein immunogens elicited several V1-2/Vκ1-33-based neutralizing antibodies that bound RBD in a different mode from each other and from those of many prior human patient-derived V1-2-based neutralizing antibodies. Of these, SP1-77 potently and broadly neutralized all SARS-CoV-2 variants through BA.5. Cryo-EM studies revealed that SP1-77 bound RBD away from the receptor-binding-motif via a CDR3-dominated recognition mode. Lattice-light-sheet-microscopy-based studies showed that SP1-77 did not block ACE2-mediated viral attachment or endocytosis, but rather blocked viral-host membrane fusion. The broad and potent SP1-77 neutralization activity and non-traditonal mechanism of action suggest this antibody might have therapeutic potential. Likewise, the SP1-77 binding epitope may further inform on vacccine strategies. Finally, the general class of humanized mouse models we have described may contribute to identifying therapeutic antibodies against future SARS-CoV-2 variants and other pathogens.
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http://dx.doi.org/10.1126/sciimmunol.add5446DOI Listing
August 2022

A Novel Approach to Assessing the Severity of Acute Stroke and Neurological Deficits in Patients with Acute Ischemic Stroke Using Myocardial Work Echocardiography.

Anatol J Cardiol 2022 Aug 11. Epub 2022 Aug 11.

Department of Ultrasound, Yangpu Hospital, School of Medicine, Tongji University, Shanghai, People's Republic of China.

Background: We aimed to evaluate the feasibility and performance of myocardial work echocardiography in assessing the severity of acute stroke and neurological deficits in patients with acute ischemic stroke.

Methods: A total of 176 patients were examined by echocardiography within 24-48 hours of symptom onset with the measurement of global and regional myocardial work. The National Institutes of Health Stroke Scale score of each patient was documented.

Results: With the increase of the National Institutes of Health Stroke Scale score, myocardial constructive work or positive work decreased (P 15 or not. The optimal cutoff value was 3.89, with a sensitivity of 100%, a specificity of 93.0%, a positive predictive value of 84.9%, a negative predictive value of 100%, and an accuracy of 95.7%.

Conclusion: Noninvasive myocardial work is highly competent in assessing the severity of acute stroke and neurological deficits, which can be used as a powerful supplement to the conventional scoring system.
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http://dx.doi.org/10.5152/AnatolJCardiol.2022.1769DOI Listing
August 2022

Development of a framework of carbon accounting and management on the township level in China.

J Environ Manage 2022 Sep 4;318:115609. Epub 2022 Jul 4.

Qingdao Research Center for Green Development and Ecological Environment, Qingdao University of Science and Technology, No.99 Songling Road, Qingdao, 266061, China.

The regional management on greenhouse gas (GHG) emissions is essential to the success of global carbon reduction. However, previous studies mostly focused on the city or country levels, while the management of GHG emissions on the township level has not been fully studied. This study proposed a framework of carbon management on the township level and developed a calculation tool for accounting GHG emissions. The case study of Lingshan Island, located in eastern China, was conducted through a site visit and interviews. A questionnaire survey was performed to collect data for carbon accounting. It is found that the island had a negative carbon emission of -1336 t COe in 2020, which is mostly contributed from energy and electricity consumption. Due to the COVID-19 pandemic, the number of tourists decreased in 2020 and the carbon emission was 224 t COe lower than in 2019. The proposed framework consists of five steps and requires the collaboration of the government, the locals, researchers and other stakeholders. The framework was validated through a focus group meeting and the suggestions on carbon management at township level were provided. This study paves a way of carbon accounting and management for the less urbanized regions.
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http://dx.doi.org/10.1016/j.jenvman.2022.115609DOI Listing
September 2022

Causal association of juvenile idiopathic arthritis-associated uveitis with depression and anxiety: a bidirectional Mendelian randomization study.

Int Ophthalmol 2022 Aug 10. Epub 2022 Aug 10.

Chongqing Key Laboratory of Ophthalmology, and Chongqing Eye Institute, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.

Purpose: The objective of this article was to examine the potential effect of juvenile idiopathic arthritis-associated uveitis (JIAU) on the risk of major depressive and anxiety disorders through Mendelian randomization (MR) study.

Methods: Genetic instrumental variables from the largest available genome-wide association study for JIAU, major depressive disorder, and anxiety disorder were applied. A set of complementary MR approaches including inverse-variance weighted (IVW) were carried out to verify the estimate association and assess horizontal pleiotropy.

Results: Our results indicated that genetically driven JIAU did not causally produce changes in major depressive or anxiety disorders (IVW: OR = 1.001, 95% CI = 0.997-1.006, P = 0.581; IVW: OR = 1.006, 95% CI = 0.980-1.033, P = 0.649, respectively). In addition, the risk of JIAU could not be influenced by genetically predicted major depressive or anxiety disorders (IVW: OR = 1.132, 95% CI = 0.914-1.404, P = 0.256; IVW: OR = 1.019, 95% CI = 0.548-1.896, P = 0.953, respectively). Besides, several sensitivity analyses indicated that our MR results were robust and no horizontal pleiotropy was observed (P > 0.05).

Conclusions: Our MR study does not reveal sufficient evidence to support the causal association of JIAU with the development of major depressive or anxiety disorders in both directions. Further large studies are warranted to validate the undetermined relationship between JIAU and the risk of major depressive or anxiety disorders.
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http://dx.doi.org/10.1007/s10792-022-02462-8DOI Listing
August 2022

Molecular mapping of quantitative trait loci for three husk traits using genotyping-by-sequencing in maize (Zea mays L.).

G3 (Bethesda) 2022 Aug 9. Epub 2022 Aug 9.

Cereal Crops Research Institute, Henan Academy of Agricultural Sciences/Henan Provincial Key Laboratory of Maize Biology, Zhengzhou 450003, China; (J.Z.); (F.Z.); (Y.D.); (J.Q.); (X.M.); (Z.M.).

The maize (Zea mays L.) husk consists of multiple leaf layers and plays an important role in grain growth and development. Despite significant achievements in physiological and morphological research, few studies have focused on the detection of genetic loci underlying husk-related traits due to the lack of efficient tools. In this study, we constructed an ultra-high-density linkage map using genotyping-by-sequence (GBS) based on a recombinant inbred line (RIL) population to estimate the genetic variance and heritability of three husk traits, i.e.,, husk width (HL), husk width (HW), and husk layer number (HN) in three field environments and the combined environment. The three husk traits showed broad phenotypic variation and high heritability, the broad-sense heritability (H2) was 0.92, 0.84 and 0.86, respectively. Twenty QTLs were consistently detected more than one environment; these viewed as stable QTLs include nine influence HL; six, HW; and five, HN. Based on the QTL mapping in the RIL population, qHL6 and qHN4 were detected across all environments and inferred to be reliable and major-effect QTLs for HL and HN, respectively. Additionally, several predicted candidate genes were identified in the region of qHL6 and qHN4, of which 17 candidate genes potentially play a role in biological processes related to development process and energy metabolism. These results will be as a useful resource for performing functional studies aimed at understanding the molecular pathways involved in husk growth and development.
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http://dx.doi.org/10.1093/g3journal/jkac198DOI Listing
August 2022

Developing and validating a chronic obstructive pulmonary disease quick screening questionnaire using statistical learning models.

Chron Respir Dis 2022 Jan-Dec;19:14799731221116585

Department of Pulmonary and Critical Care Medicine, 92323Zhongshan Hospital, Fudan University, Shanghai, China.

Background: Active targeted case-finding is a cost-effective way to identify individuals with high-risk for early diagnosis and interventions of chronic obstructive pulmonary disease (COPD). A precise and practical COPD screening instrument is needed in health care settings.

Methods: We created four statistical learning models to predict the risk of COPD using a multi-center randomized cross-sectional survey database ( = 5281). The minimal set of predictors and the best statistical learning model in identifying individuals with airway obstruction were selected to construct a new case-finding questionnaire. We validated its performance in a prospective cohort ( = 958) and compared it with three previously reported case-finding instruments.

Results: A set of seven predictors was selected from 643 variables, including age, morning productive cough, wheeze, years of smoking cessation, gender, job, and pack-year of smoking. In four statistical learning models, generalized additive model model had the highest area under curve (AUC) value both on the developing cross-sectional data set (AUC = 0.813) and the prospective validation data set (AUC = 0.880). Our questionnaire outperforms the other three tools on the cross-sectional validation data set.

Conclusions: We developed a COPD case-finding questionnaire, which is an efficient and cost-effective tool for identifying high-risk population of COPD.
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http://dx.doi.org/10.1177/14799731221116585DOI Listing
August 2022

Effect of Intravenous Tirofiban vs Placebo Before Endovascular Thrombectomy on Functional Outcomes in Large Vessel Occlusion Stroke: The RESCUE BT Randomized Clinical Trial.

JAMA 2022 08;328(6):543-553

Department of Neurology, Nanyang Central Hospital, Nanyang, China.

Importance: Tirofiban is a highly selective nonpeptide antagonist of glycoprotein IIb/IIIa receptor, which reversibly inhibits platelet aggregation. It remains uncertain whether intravenous tirofiban is effective to improve functional outcomes for patients with large vessel occlusion ischemic stroke undergoing endovascular thrombectomy.

Objective: To assess the efficacy and adverse events of intravenous tirofiban before endovascular thrombectomy for acute ischemic stroke secondary to large vessel occlusion.

Design, Setting, And Participants: This investigator-initiated, randomized, double-blind, placebo-controlled trial was implemented at 55 hospitals in China, enrolling 948 patients with stroke and proximal intracranial large vessel occlusion presenting within 24 hours of time last known well. Recruitment took place between October 10, 2018, and October 31, 2021, with final follow-up on January 15, 2022.

Interventions: Participants received intravenous tirofiban (n = 463) or placebo (n = 485) prior to endovascular thrombectomy.

Main Outcomes And Measures: The primary outcome was disability level at 90 days as measured by overall distribution of the modified Rankin Scale scores from 0 (no symptoms) to 6 (death). The primary safety outcome was the incidence of symptomatic intracranial hemorrhage within 48 hours.

Results: Among 948 patients randomized (mean age, 67 years; 391 [41.2%] women), 948 (100%) completed the trial. The median (IQR) 90-day modified Rankin Scale score in the tirofiban group vs placebo group was 3 (1-4) vs 3 (1-4). The adjusted common odds ratio for a lower level of disability with tirofiban vs placebo was 1.08 (95% CI, 0.86-1.36). Incidence of symptomatic intracranial hemorrhage was 9.7% in the tirofiban group vs 6.4% in the placebo group (difference, 3.3% [95% CI, -0.2% to 6.8%]).

Conclusions And Relevance: Among patients with large vessel occlusion acute ischemic stroke undergoing endovascular thrombectomy, treatment with intravenous tirofiban, compared with placebo, before endovascular therapy resulted in no significant difference in disability severity at 90 days. The findings do not support use of intravenous tirofiban before endovascular thrombectomy for acute ischemic stroke.

Trial Registration: Chinese Clinical Trial Registry Identifier: ChiCTR-IOR-17014167.
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http://dx.doi.org/10.1001/jama.2022.12584DOI Listing
August 2022

High-Pressure Synthesis and Physical Properties of a Spinel Compound FeAlS.

Inorg Chem 2022 Aug 9. Epub 2022 Aug 9.

Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.

A spinel compound FeAlS was successfully synthesized under high-pressure and high-temperature conditions and was systematically characterized via the structural, magnetic, and specific heat measurements. It crystallizes into a cubic structure with the space group 3̅ (no. 227) and the lattice constant = 10.0207(2) Å. A Fe/Al site inversion is found; that is, the molecular formula can be rewritten as (FeAl)(AlFe)S, and the inversion parameter is about 0.22. Magnetic susceptibility measurements indicate that FeAlS undergoes a spin glass behavior, which is confirmed by ac susceptibility and specific heat measurements. The freezing temperature ∼ 10.5 K and Weiss temperature ∼ -107.4 K lead to a high frustration parameter = |/| of about 10, which suggests that FeAlS is a high-frustration magnet. Our results indicate that high pressure can help stabilize the spinel structure with small and the cation inversion plays an important role in the formation of the spin glass state.
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http://dx.doi.org/10.1021/acs.inorgchem.2c02034DOI Listing
August 2022

Human adipose-derived mesenchymal stem cells-derived exosomes encapsulated in pluronic F127 hydrogel promote wound healing and regeneration.

Stem Cell Res Ther 2022 Aug 8;13(1):407. Epub 2022 Aug 8.

Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200092, China.

Background: Large area skin trauma has always been a great challenge for both patients and clinicians. Exosomes originating from human adipose-derived mesenchymal stem cells (hADSCs) have been a novel promising cell-free treatment in cutaneous damage repair. Nevertheless, the low retention rate of exosomes post-transplantation in vivo remains a significant challenge in clinical applications. Herein, we purposed to explore the potential clinical application roles of hADSCs-Exos encapsulated in functional PF-127 hydrogel in wound healing.

Methods: hADSCs-Exos were isolated from human hADSCs by ultracentrifugation. An injectable, biocompatible, and thermo-sensitive hydrogel Pluronic F-127 hydrogel was employed to encapsulate allogeneic hADSCs-Exos, and this complex was topically applied to a full-thickness cutaneous wound in mice. On different days post-transplantation, the mice were sacrificed, and the skin tissue was excised for histological and immunohistochemical analysis.

Results: Compared with hADSCs-Exos or PF-127 only, PF-127/hADSCs-Exos complexes enhanced skin wound healing, promoted re-epithelialization, increased expression of Ki67, α-SMA, and CD31, facilitated collagen synthesis (Collagen I, Collagen III), up-regulated expression of skin barrier proteins (KRT1, AQP3), and reduced inflammation (IL-6, TNF-α, CD68, CD206). By using PF-127/hADSCs-Exos complexes, hADSCs-Exos can be administrated at lower doses frequency while maintaining the same therapeutic effects.

Conclusion: Administration of hADSCs-Exos in PF-127 improves the efficiency of exosome delivery, maintains the bioactivity of hADSCs-Exos, and optimizes the performance of hADSCs-Exos. Thus, this biomaterial-based exosome will be a promising treatment approach for the cutaneous rejuvenation of skin wounds.
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http://dx.doi.org/10.1186/s13287-022-02980-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9358082PMC
August 2022

Persistent versus transient, and conventional plastic versus biodegradable plastic? -Two key questions about microplastic-water exchange of antibiotic resistance genes.

Water Res 2022 Jul 26;222:118899. Epub 2022 Jul 26.

Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou 310014, China. Electronic address:

The ubiquitous microplastics (MPs) in water environment play an important role in the dissemination of antibiotic resistance genes (ARGs) due to their exchange between floating MPs and receiving waters. However, whether the ARG exchange is persistent or transient and what are the differences in ARG exchange between conventional plastics and biodegradable plastics are the two key issues to be addressed. In this study, biodegradable PBAT and non-biodegradable PET MPs were chosen to explore the MP-water ARG exchange after the MPs floated to the receiving waters. The results demonstrated that the active exchange of ARGs between MPs and receiving waters occurred, which, however, were transient for most of ARGs. The relative abundance of ARGs both on the MPs and in the waters rapidly decreased to the initial or lower levels within 4 weeks. Approximately 25-50% (ARG subtype number ratio) of studied ARG subtypes were introduced into the receiving waters by MPs, and 35-65% of studied ARG subtypes went through fluctuation in terms of abundance on MPs and in the receiving water. ARGs tended to converge between MPs and the receiving waters with time. Furthermore, the ARG exchange between MPs and waters facilitated horizontal gene transfer (HGT). IntI1 and tnpA05 played the crucial roles in HGT, which was indicated by their correlated change with most ARGs; in contrast, tnpA04 showed the obvious lagging responses. The biodegradable MP of PBAT generally accumulated higher levels of most ARGs including multidrug resistant genes than the non-biodegradable MP of PET. The transient exchange of most ARGs between MPs and water implies that the on-off hitchhiking of ARGs on MPs in aquatic environment may not exert significant influence on ARG transmission. However, compared with the conventional plastics, the biodegradable MPs might pose much higher ARG dissemination risks due to the higher enrichment of ARGs particularly with people's ever-increasingly usage. Enough attention must be paid to this emerging issue.
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http://dx.doi.org/10.1016/j.watres.2022.118899DOI Listing
July 2022

Initial screening of gastric cancer using oral contrast-enhanced trans-abdominal ultrasonography in rural asymptomatic individuals.

Cancer Epidemiol 2022 Aug 5;80:102236. Epub 2022 Aug 5.

Department of Oncology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197 Ruijin Er Road, Shanghai 200025, China. Electronic address:

Background & Aims: Initial screening for high-risk population of gastric cancer (GC) is needed in rural areas of large-population countries. This study aims to explore the feasibility of applying noninvasive ultrasonography as an initial screening strategy to improve the early diagnosis and prevention of GC.

Methods: Oral contrast-enhanced trans-abdominal ultrasonography (OCTU) was initially applied to screen around 15,000 residents from 24 different rural villages of Changxing Island in Shanghai, China, facilitating the identification of high-risk population for further endoscopy examination.

Results: 176 subjects (1.18 %) were initially identified with gastric diseases using OCTU while 14,787 ones (98.93 %) were normal with negative results. 145 out of 176 individuals (82.39 %) took further endoscopy examination, and 16 were diagnosed with GC with biopsy examination, with 9 of them at the early stage. We followed up with the Center for Disease Control and Prevention, and identified another 6 GC cases occurred within one year among OCTU-negative population, serving as an adjustment factor for sensitivity analysis. As a result, with a total of 22 GC cases included in this cohort, the positive predictive rate, the negative predictive value, sensitivity, and specificity were 9.09%, 99.96 %, 75.5 %, and 98.93 %, respectively.

Conclusions: OCTU is feasible, non-invasive, low-cost, and widely acceptable in rural area, thus we proposed that OCTU is practicable to serve as a supplementary screening method to improve the early detection of GC in rural area of China and other developing countries with large population.
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http://dx.doi.org/10.1016/j.canep.2022.102236DOI Listing
August 2022

PPISB: A Novel Network-Based Algorithm of Predicting Protein-Protein Interactions With Mixed Membership Stochastic Blockmodel.

IEEE/ACM Trans Comput Biol Bioinform 2022 Aug 8;PP. Epub 2022 Aug 8.

Protein-protein interactions (PPIs) play an essential role for most of biological processes in cells. Many computational algorithms have thus been proposed to predict PPIs. However, most of them heavily rest on the biological information of proteins while ignoring the latent structural features of proteins presented in a PPI network. In this paper, we propose an efficient network-based prediction algorithm, namely PPISB, based on a mixed membership stochastic blockmodel. By simulating the generative process of a PPI network, PPISB is able to capture the latent community structures. The inference procedure adopted by PPISB further optimizes the membership distributions of proteins over different complexes. After that, a distance measure is designed to compute the similarity between two proteins in terms of their likelihoods of being in the same complex, thus verifying whether they interact with each other or not. To evaluate the performance of PPISB, a series of extensive experiments have been conducted with five PPI networks collected from different species and the results demonstrate that PPISB has a promising performance when applied to predict PPIs in terms of several evaluation metrics. Hence, we reason that PPISB is preferred over state-of-the-art network-based prediction algorithms especially for predicting potential PPIs.
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http://dx.doi.org/10.1109/TCBB.2022.3196336DOI Listing
August 2022

Reducing Homelessness among Persons with HIV: An Ecological Case Study in Delaware.

J HIV AIDS Soc Serv 2022 Dec;21(1):1-15

Division of HIV/AIDS Prevention, Centers for Disease Control and Prevention, Atlanta GA.

Introduction: Among persons with HIV (PWH), homelessness is associated with poorer health. From 2009-2014, national HIV prevention goals included a reduction in homelessness among PWH. We sought to examine social ecological factors associated with homelessness among PWH at a sub-national level during that period.

Methods: National data were used to identify Delaware as the only jurisdiction where homelessness among PWH declined from 2009-2014. We analyzed population-level indicators and conducted telephone interviews with 6 key stakeholders to further examine this trend.

Results: Overall homelessness, household poverty, and median housing price were associated with homelessness among PWH in Delaware. Key stakeholders indicated that centralized intake processes improved screening, referral, and linkages of clients to housing units.

Discussion: In addition to social and economic factors, collaborative program strategies may improve housing outcomes for PWH. Monitoring trends at sub-national levels can help identify successful approaches as well as needed services or policy change.
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http://dx.doi.org/10.1080/15381501.2021.2015502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348807PMC
December 2022

Case report: Double-chambered right ventricle diagnosed in a middle-aged female with hypertrophic cardiomyopathy and atrial flutter: A rare case.

Front Cardiovasc Med 2022 22;9:937758. Epub 2022 Jul 22.

The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education, Chinese National Health Commission and Chinese Academy of Medical Sciences, The State and Shandong Province Joint Key Laboratory of Translational Cardiovascular Medicine, Department of Cardiology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Double-chambered right ventricle (DCRV) is a rare congenital heart defect in adults, manifesting with progressive right ventricular outflow tract obstruction. We describe the first case of DCRV coexisting with hypertrophic cardiomyopathy, which is complicated by atrial flutter. A middle-aged woman with recurrent symptomatic atrial flutter who had previously been diagnosed with biventricular hypertrophic cardiomyopathy was admitted to our department. Echocardiography and cardiac magnetic resonance revealed asymmetrical interventricular septal hypertrophy, and abnormal muscle bundles within the right ventricle, generating an obstructive gradient. Genetic testing detected a hypertrophic cardiomyopathy-associated mutation: , c.4135G > A, p. Ala1379Thr. A diagnosis of DCRV complicated by hypertrophic cardiomyopathy and atrial flutter was made. Surgical intervention was performed, which included radiofrequency ablation, removal of abnormal muscle bundles, and ventricular septal defect repair. Intraoperative transesophageal echocardiography demonstrated the well-corrected right ventricular outflow tract. Free of early postoperative complications, the patient was discharged in sinus rhythm on the 11th day after the surgery. Unfortunately, the patient died from a sudden death 38 days following the surgery. In conclusion, the coexistence of DCRV with hypertrophic cardiomyopathy in patients is an uncommon condition. The present case highlights the importance of diagnostic imaging in the management of this disorder.
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http://dx.doi.org/10.3389/fcvm.2022.937758DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353184PMC
July 2022

Evaluation of degradation performance toward antiviral drug ribavirin using advanced oxidation process and its relations to ecotoxicity evolution.

Sci Total Environ 2022 Aug 4:157851. Epub 2022 Aug 4.

Center for Water and Ecology, State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China. Electronic address:

The rapid spread of coronavirus disease 2019 has increased the consumption of some antiviral drugs, wherein these are discharged into wastewater, posing risks to the ecosystem and human health. Therefore, efforts are being made for the development of advanced oxidation processes (AOPs) to remediate water containing these pharmaceuticals. Here, the toxicity evolution of the antiviral drug ribavirin (RBV) was systematically investigated during its degradation via the UV/TiO/HO advanced oxidation process. Under optimal conditions, RBV was almost completely eliminated within 20 min, although the mineralization rate was inadequate. Zebrafish embryo testing revealed that the ecotoxicity of the treated RBV solutions increased at some stages and decreased as the reaction time increased, which may be attributed to the formation and decomposition of various transformation products (TPs). Liquid chromatography-mass spectrometry analysis along with density functional theory calculations helped identify possible toxicity increase-causing TPs, and quantitative structure activity relationship prediction revealed that most TPs exhibit higher toxicity than the parent compound. The findings of this study suggest that, in addition to the removal rate of organics, the potential ecotoxicity of treated effluents should also be considered when AOPs are applied in wastewater treatment.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157851DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9351291PMC
August 2022

Association of ambient fine particulate matter exposure with gestational diabetes mellitus and blood glucose levels during pregnancy.

Environ Res 2022 Aug 2:114008. Epub 2022 Aug 2.

Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan, Shanxi, China. Electronic address:

Background: Previous studies have examined the associations between ambient fine particulate matter (PM) exposure and gestational diabetes mellitus (GDM). However, limited studies explored the relationships between PM exposure and blood glucose levels during pregnancy, especially in highly polluted areas.

Objectives: To examine the associations of prenatal ambient PM exposure with GDM and blood glucose levels, and to identify the sensitive exposure windows in a highly air-polluted area.

Methods: From July 2016 to October 2017, a birth cohort study was conducted in Beijing, China. Participants were interviewed in each trimester regarding demographics, lifestyle, living and working environment, and medical conditions. Participant's daily ambient PM levels from 3 m before last menstrual period (LMP) to the third trimester was estimated by a hybrid spatiotemporal model. Indoor air quality index was calculated based on environmental tobacco smoke, ventilation, cooking, painting, pesticide, and herbicide use. Distributed lag non-linear model was applied to explore the sensitive weeks of PM exposure.

Results: Of 165 pregnant women, 23 (13.94%) developed GDM. After adjusting for potential confounders, PM exposure during the 1st trimester was associated with higher odds of GDM (10 μg/m increase: OR = 1.89, 95% CI: 1.04-3.49). Each 10 μg/m increase in PM during the 2nd trimester was associated with 17.70% (2.21-33.20), 15.99% (2.96-29.01), 18.82% (4.11-33.52), and 17.10% (3.28-30.92) increase in 1-h, 2-h, Δ1h-fasting (1-h minus fasting), and Δ2h-fasting (2-h minus fasting) blood glucose levels, respectively. PM exposure at 24th-27th weeks after LMP was associated with increased GDM risk. We identified sensitive exposure windows of 21st-24th weeks for higher 1-h and 2-h blood glucose levels and of 20th-22nd weeks for increased Δ1h-fasting and Δ2h-fasting.

Conclusions: Ambient PM exposure during the second trimester was associated with higher odds of GDM and higher blood glucose levels. Avoiding exposure to high air pollution levels during the sensitive windows might prevent women from developing GDM.
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http://dx.doi.org/10.1016/j.envres.2022.114008DOI Listing
August 2022

Homeodomain-interacting protein kinase HIPK4 regulates phosphorylation of manchette protein RIMBP3 during spermiogenesis.

J Biol Chem 2022 Aug 2:102327. Epub 2022 Aug 2.

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 211166, China. Electronic address:

Nonobstructive azoospermia (NOA) is the most serious form of spermatogenesis abnormalities in male infertility. Genetic factors are important to consider as elements leading to NOA. Although many pathogenic genes have been reported, the causative genes of NOA for many patients are still unknown. In this study, we found ten point mutations in the gene encoding homeodomain-interacting protein kinase 4 (HIPK4) in patients with NOA, and using in vitro studies we determined a premature termination point mutation (p. Lys490*, c.1468A>T) that can cause decreased expression of HIPK4. Our phosphoproteomic analysis of Hipk4 testes revealed phosphorylation of multiple proteins regulated by HIPK4 during spermiogenesis. We also confirmed that a substrate of HIPK4 with four down-regulated phosphorylation sites matching the xSPx motif is the known manchette-related protein RIMS-binding protein 3 (RIMBP3), which is required for sperm head morphogenesis. Therefore, we conclude HIPK4 regulates the phosphorylation of manchette protein RIMBP3 and plays essential roles in sperm head shaping and male fertility.
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http://dx.doi.org/10.1016/j.jbc.2022.102327DOI Listing
August 2022

Development and validation of comprehensive clinical outcome prediction models for acute ischaemic stroke in anterior circulation based on machine learning.

J Clin Neurosci 2022 Aug 3;104:1-9. Epub 2022 Aug 3.

Department of Radiology, Huashan Hospital, Fudan University, Shanghai, China; Academy for Engineering and Technology, Fudan University, Shanghai, China. Electronic address:

The current prediction models for the clinical outcome of acute ischaemic stroke (AIS) remain insufficient for individualized patient management strategies. We aimed to investigate machine learning (ML) performance in the clinical outcome prediction of AIS in anterior circulation and evaluate the clinical outcome by combining the quantitative evaluation indicators of perfusion features and basic clinical information. Four ML classifiers, support vector machine (SVM), naive Bayes (NB), logistic regression (LR), and random forest (RF) were trained on a cohort of 389 adult patients (training cohort [70 %]; external validation cohort [30 %]) from the Acute Stroke Center Registry of Huashan Hospital. Model performance was compared by a range of learning metrics. Most imaging parameters were strongly correlated with the outcome (range, 0.57 to 0.81), and the correlation between relative cerebral blood flow (rCBF) < 30 % and clinical outcome was the strongest (ρ = 0.81). As the reference parameters increased, the performance of the four models was greatly improved [SVM (AUC: from 0.79 to 0.99, F1-score: from 0.61 to 0.90), RF (AUC: from 0.88 to 0.98, F1-score: from 0.71 to 0.96), LR (AUC: from 0.80 to 0.97, F1-score: from 0.64 to 0.95), and NB (AUC: from 0.74 to 0.97, F1-score: from 0.66 to 0.92)]. The ensemble classifier model with all parameters had the highest F1-score (0.97). All the ML models, jointly considering the basic clinical information and quantitative evaluation indicators of computed tomography perfusion (CTP), showed good performance in the prediction of clinical outcome of AIS in anterior circulation.
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http://dx.doi.org/10.1016/j.jocn.2022.07.022DOI Listing
August 2022

[A consensus on prenatal diagnosis and genetic counseling for chromosomal mosaicism].

Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2022 Aug;39(8):797-802

The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China.

With the extensive application of highly sensitive genetic techniques in the field of prenatal diagnosis, prenatal chromosomal mosaicisms including true fetal mosaicisms and confined placental mosaicisms are frequently identified in clinical settings, and the diagnostic criteria and principle of genetic counseling and clinical management for such cases may vary significantly among healthcare centers across the country. This not only has brought challenges to laboratory technician, genetic counselor and fetal medicine doctor, but can also cause confusion and anxiety of the pregnant woman and their family members. In this regard, we have formulated a consensus over the prenatal diagnosis and genetic counseling for chromosomal mosaicisms with the aim to promote more accurate and rational evaluation for fetal chromosomal mosaicisms in prenatal clinics.
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http://dx.doi.org/10.3760/cma.j.cn511374-20210930-00794DOI Listing
August 2022

The effect of Lactobacillus rhamnosus B10 on alcoholic liver injury and intestinal microbiota in alcohol-induced mice model.

J Food Biochem 2022 Aug 5:e14372. Epub 2022 Aug 5.

College of Food Science and Engineering, Jilin Agricultural University, Changchun, China.

Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota. C57BL/6N mice were fed with liquid diet Lieber-DeCarli with or without 5% (v/v) ethanol for 8 weeks, and treated with L. rhamnosus B10 at the last 2 weeks. The results showed that L. rhamnosus B10 decreased the serum total cholesterol (1.48 mmol/L), triglycerides (0.97 mmol/L), alanine aminotransferase (26.4 U/L), aspartate aminotransferase (14.2 U/L), lipopolysaccharide (0.23 EU/mL), and tumor necrosis factor-α (138 pg/mL). In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury. The changes of intestinal microbiota composition on class, family and genus level in cecum were analyzed. The intestinal symbiotic abundance of Firmicutes was elevated while gram-negative bacteria Proteobacteria and Deferribacteres was decreased in alcohol mice treated with L. rhamnosus B10 for 2 weeks. In summary, this study provided evidence for the therapeutic effects of probiotics on alcoholic liver injury by regulating intestinal flora.
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http://dx.doi.org/10.1111/jfbc.14372DOI Listing
August 2022

Effects of mir-195 Targeted Regulation of JAK2 on Proliferation, Invasion, and Apoptosis of Gastric Cancer Cells.

Comput Math Methods Med 2022 26;2022:5873479. Epub 2022 Jul 26.

Department of Gastroenterology, Xi'an Central Hospital, Xi'an, 710003 Shaanxi, China.

Background: Overexpression of miR-195 can make gastric cancer cells stay in G1/G2 phase. miR-195 has been shown to inhibit gastric cancer cell replication and accelerate cell death by targeting JAK2. However, the relationship between miR-195, JAK2, and gastric cancer is not clear.

Objective: To observe the effect of mir-195 regulated by JAK2 on the growth, invasion, and death of gastric cancer cells.

Methods: MGC803 and NCI gastric N87 cells were introduced into the negative control sequences of miR-195 and RNA, respectively. To detect the expression of miR-195 in cells, to detect the effect of miR-195 on mitosis and proliferation of tumor cells, to analyze the effect of miR-195 on cell invasion and metastasis, and to detect the regulation of miR-195 on JAK2 expression.

Results: The level of miR-195 in miR-195-MIMICS group was significantly higher than that in miR-NC group. The cell survival rate of miR-195 mimic group was lower than that of miR-NC group ( < 0.05). Compared with miR-NC group, the number of cells in G1 phase increased, the cells in G2 phase and S phase decreased, and the proportion of cells in G2 and S phase decreased in miR-195 mimic group. The scratch distance of miR-195 simulator group was larger than that of control group. The number of invasive cells in the miR-195 mimic group was significantly lower than that in the control group. The expression of JAK2 protein in miR-195 mimic group was lower than that in miR-NC group. There was a significant negative correlation between the expression level of miR-195 and JAK2 (rhabdomile 0.326 and record 0.00). There are continuous interaction fragments between JAK2 and miR-195. The luciferase activity of miR-195 mimic and wild type JAK2 sequence expression vector was significantly lower than that of wild type JAK2 sequence expression vector.

Conclusion: miR-195 may inhibit the occurrence, metastasis, and invasion of gastric tumor by downregulating the expression of JAK2. miR-195/JAK2 may be a new molecular target for the treatment of gastrointestinal tumors.
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http://dx.doi.org/10.1155/2022/5873479DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345721PMC
August 2022

Use of failure mode and effect analysis to reduce patient safety risks in purchasing prescription drugs from online pharmacies in China.

Front Med (Lausanne) 2022 19;9:913214. Epub 2022 Jul 19.

Administration for Market Regulation of Chengdu, Chengdu, China.

Background: Online pharmacies have gradually penetrated the market, but pose risks to patients' health. Failure Mode and Effect Analysis (FMEA) is an effective and reliable method for reducing pharmacy and medication risks. The purpose of this study was to conduct a prospective risk analysis of the process of purchasing prescription drugs from online pharmacies in China to guarantee drug quality and patient safety.

Methods: The FMEA was performed at Sichuan University, China. A multidisciplinary team was assembled comprising a leader, four regulators, four pharmacists, two experts, etc. The process was composed of eight subprocesses: searching for prescription drugs, submitting medication requirements, completing patient information forms, dispensing, delivering, etc. Brainstorming was used to identify and prioritize failure modes, propose corrective actions, and reduce risks. Risk priority numbers were the main criterion and were obtained by multiplying three scores: severity, occurrence and detectability, which were scored by the team The team proposed corrective actions for each selected failure mode.

Results: A total of forty-one potential failure modes were identified, and the causes, effects, and corrective actions of the 30 top failure modes were analyzed. The highest risk value was assigned to "photocopies of paper prescriptions uploaded were reused by patients." Three failure modes for the S value of 5 were: "drugs are eroded and polluted by moisture or insects in the process of transportation," "the qualification information of the pharmacies were absent or fake," and "pharmacists fail to check prescriptions in accordance with Prescription Administrative Regulation." Of the top failure modes, 36.67% were from Step 5, delivering the drug. After taking corrective measures to control risks, the risks reduced by 69.26%.

Conclusion: The results of this study proves that the FMEA is a valuable tool for identifying and prioritizing the risks inherent in online pharmacies. This study shows that there are many potential risks in the process of purchasing prescription drugs from online pharmacies, especially in the drug delivery stage. Enhanced training and the introduction of smart devices may minimize risks. Online pharmacies and Chinese regulators should consider these findings for risk mitigation and the improvement of regulations pertaining to online pharmacies.
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http://dx.doi.org/10.3389/fmed.2022.913214DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9343844PMC
July 2022

Protective effect and mechanism of cannabidiol on myocardial injury in exhaustive exercise training mice.

Chem Biol Interact 2022 Aug 1:110079. Epub 2022 Aug 1.

Shenyang Medical College, No. 146, Huanghe North Street, Shenyang, 110034, China. Electronic address:

Cannabinoid diphenol (CBD) is a non-toxic main component extracted from cannabis, which has the effects of anti-inflammatory, anti-apoptosis and anti-oxidative stress. In recent years, exercise-induced myocardial injury has become a research hotspot in the field of sports medicine and sports physiology. Exercise-induced myocardial injury is closely related to oxidative stress, inflammatory response and apoptosis. However, there is no clear evidence of the relationship between CBD and exercise-induced myocardial injury. In this study, by establishing an animal model of exhaustive exercise training in mice, the protective effect of CBD on myocardial injury in mice was elaborated, and the possible molecular mechanism was discussed. After CBD intervention, the arrangement and rupture of myocardial fiber tissue and the degree of inflammatory cell infiltration were reduced, the deposition of collagen fibers in myocardial tissue decreased. CBD can also significantly inhibit cardiac hypertrophy. Meanwhile, the expression of IL-6, IL-10, TNF-α, Bax, Caspase-3, Bcl-2, MDA-5, IRE-1α, NOX-2, SOD-1, Keap1, Nrf2, HO-1, NF-κB and COX-2 was recovered to normal. In addition, after CBD intervention, the protein expression of Keap1 was down-regulated, the translocation of Nrf2 from the cytoplasm to the nucleus was significantly increased, then the transcriptional activity was increased, and the expression of the downstream HO-1 antioxidant protein was increased, indicating that CBD may improve the cardiac function of exhaustive exercise training mice by activating Keap1/Nrf2/HO-1 signaling pathway. Molecular docking results also confirmed that CBD had a good binding effect with Keap1/Nrf2/HO-1 signaling pathway proteins. In conclusion, the protective mechanism of CBD on myocardial injury in exhaustive exercise training mice may be to activate Keap1/Nrf2/HO-1 signaling pathway, and then exert anti-inflammatory, anti-apoptosis and inhibition of oxidative stress.
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http://dx.doi.org/10.1016/j.cbi.2022.110079DOI Listing
August 2022

[Design and application of a multi-assisted function incision and orifice closure buckle].

Zhonghua Wei Zhong Bing Ji Jiu Yi Xue 2022 Jun;34(6):651-652

Department of Neurology, Clinical Medical College of Yangzhou University, Yangzhou 225001, Jiangsu, China.

Incision infection is one of the common complications in surgery. Infected incisions usually need to perform procedures including suture removal, debridement, drainage, sterilization and anti-inflammatory. Until, the wound edge was sutured again after the wound infection was controlled. This contributes to considerable physical and psychological suffering for patients. To this end, with Dalian Medical University as the main inventor and other several experts, a multi-assistance function incision and orifice closure buckle have been designed and obtained the national utility model patent (patent number: ZL 2019 2 1803918.4). The closure buckle with was composed of two blocks with an adhesive layer and one tensioning mechanism. The device is easy to operate, and could effectively play an analgesic, antibacterial and promote healing on the basis of perfecting its wound margins and orifice. It has certain clinical application value.
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http://dx.doi.org/10.3760/cma.j.cn121430-20211107-01648DOI Listing
June 2022

The Relationship Between Porphyromonas Gingivalis and Rheumatoid Arthritis: A Meta-Analysis.

Front Cell Infect Microbiol 2022 18;12:956417. Epub 2022 Jul 18.

Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, China.

Rheumatoid arthritis (RA) is a systematical autoimmune disease, characterized by chronic synovial joint inflammation and hurt. () can cause life-threatening inflammatory immune responses in humans when the host pathogenic clearance machinery is disordered. Some epidemiological studies have reported that exposure would increase the prevalence of RA. However, the results remain inconsistent. Therefore, a meta-analysis was done to systematically analyze the relationship between exposure and the prevalence of rheumatoid arthritis. Database including Cochrane Library, Web of Science, PubMed, and EMBASE were searched for published epidemiological articles assessed the relationship between and RA. Obtained studies were screened based on the predefined inclusion and exclusion criteria. The overall Odds Ratios (ORs) of incorporated articles were pooled by random-effect model with STATA 15.1 software. The literature search returned a total of 2057 studies. After exclusion, 28 articles were included and analyzed. The pooled ORs showed a significant increase in the risk of RA in individuals with exposure (OR = 1.86; 95% CI: 1.43-2.43). Subgroup analysis revealed that pooled ORs from populations located in Europe (OR = 2.17; 95% CI: 1.46-3.22) and North America (OR = 2.50; 95% CI: 1.23-5.08) were significantly higher than that from population in Asia (OR = 1.11; 95% CI: 1.03-1.20). Substantial heterogeneity was observed but did not significantly influence the overall outcome. In conclusion, our results indicated exposure was a risk factor in RA. Prompt diagnosis and management decisions on antimicrobial therapy would prevent rheumatoid arthritis development and progression.
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http://dx.doi.org/10.3389/fcimb.2022.956417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340274PMC
August 2022

Identification of 12 OCA Cases in Chinese Population and Two Novel Variants.

Front Genet 2022 12;13:926511. Epub 2022 Jul 12.

Research Center for Translational Medicine, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, China.

OCA (oculocutaneous albinism) refers to a group of heterogeneous congenital disorders of which the common manifestations are variable degrees of cutaneous hypopigmentation and significant visual impairment, including poor visual acuity, photophobia, and nystagmus. Molecular analysis may elucidate its pathogenesis and be in favor of accurate diagnosis. High-throughput sequencing and Sanger sequencing were performed to detect mutational alleles and analysis was performed for prediction of variant pathogenicity. Ten -related and two -related patients were identified with 16 different variants with potential pathogenicity. Two novel missense variants [: c.623T > G, (Leu208Arg) and : c.1325A > G, (Asn442Ser)] are identified in this study, and three OCA cases are reported for the first time in Chinese population based on their associated variants. Analysis of crystal structures of TYR ortholog and its paralog TYRP1 suggests that the substitution of Leu may have an impact on protein stability. This study may facilitate OCA diagnosis by expanding the mutational spectrum of and as well as further basic studies about these two genes.
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http://dx.doi.org/10.3389/fgene.2022.926511DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9340472PMC
July 2022

Existing barriers and recommendations of real-world data standardisation for clinical research in China: a qualitative study.

BMJ Open 2022 08 3;12(8):e059029. Epub 2022 Aug 3.

Merck & Co Inc, Rahway, New Jersey, USA.

Objective: To investigate the existing barriers and recommendations of real-world data (RWD) standardisation for clinical research through a qualitative study on different stakeholders.

Design: This qualitative study involved five types of stakeholders based on five interview outlines. The data analysis was performed using the constructivist grounded theory analysis process.

Setting: Eight hospitals, four hospital system vendors, three big data companies, six medical products companies and four regulatory institutions were included.

Participants: In total, 62 participants from 25 institutions were interviewed through purposive sampling.

Results: The findings showed that the lack of clinical applicability in existing terminology standards, lack of generalisability in existing research databases, and lack of transparency in existing data standardisation process were the barriers of data standardisation of RWD for clinical research. Enhancing terminology standards by incorporating locally used clinical terminology, reducing burden in the usage of terminology standards, improving generalisability of RWD for research by using clinical data models, and improving traceability to source data for transparency might be feasible suggestions for solving the current problems.

Conclusions: Efficient and reliable data standardisation of RWD for clinical research can help generate better evidence used to support regulatory evaluation of medical products. This research suggested enhancing terminology standards by incorporating locally used clinical terminology, reducing burden in the usage of terminology standards, improving generalisability of RWD for research by using clinical data models, and improving traceability to source data for transparency to guide efforts in data standardisation in the future.
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http://dx.doi.org/10.1136/bmjopen-2021-059029DOI Listing
August 2022

Association of CDK6 gene polymorphisms with Behcet's disease in a Han Chinese population.

Exp Eye Res 2022 Jul 31:109203. Epub 2022 Jul 31.

Department of Ophthalmology, The Second Affiliated Hospital of Fujian Medical University, Engineering Research Center of Assistive Technology for Visual Impairment, Fujian Province University, Quanzhou, PR China; Department of Ophthalmology & Optometry, The School of Medical Technology and Engineering, Fujian Medical University, Fuzhou, PR China. Electronic address:

Cyclin-dependent kinases 4/6 (CDK4/6) and D1-type cyclins (CCND1) can regulate the pro-inflammatory functions of various cytokines during the inflammatory response. This study investigated the association between CDK4/6-CCND1 variants and susceptibility in patients with Behcet's disease (BD). This case-control study enrolled 542 patients with BD and 754 healthy controls. Fourteen tagged single nucleotide polymorphisms (tag SNPs) of the CDK4/6-CCND1 gene were genotyped using the Sequenom MassARRAY system and iPLEX® Pro assay. The results indicated that the frequency of the CDK6 rs2282983 TT genotype was higher in the BD group than the control group (Pc = 0.040, OR = 1.408, 95% CI = 1.124-1.765), and CDK6 rs2282983 CT and rs42034 AG were negatively associated with BD (Pc = 3.647 × 10, OR = 0.598, 95% CI = 0.471-0.758; Pc = 0.039, OR = 0.626, 95% CI = 0.459-0.852, respectively). Furthermore, statistical analysis showed that CDK6 rs2282983 TT and CT genotypes were significantly associated with skin lesions in patients with BD (Pc = 0.042, OR = 1.436, 95% CI = 1.130-1.824; Pc = 0.001, OR = 0.594, 95% CI = 0.461-0.764, respectively). This study suggests that the CDK6 loci rs2282983 and rs42034 might confer genetic susceptibility to BD in a Han Chinese population, which could provide new insights into the pathogenesis of BD.
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http://dx.doi.org/10.1016/j.exer.2022.109203DOI Listing
July 2022

Probing longitudinal carrier transport in perovskite thin films modified transient reflection spectroscopy.

Chem Sci 2022 Jul 21;13(28):8334-8340. Epub 2022 Jun 21.

State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences Dalian 116023 China

Accurate characterization of the longitudinal (along the thickness direction) carrier transport property is of significant importance for evaluating the quality and performance of perovskite thin films. Herein, we report the development of a modified transient reflection (TR) spectroscopy method to realize the direct observation and determination of the longitudinal carrier transport process in MAPbI polycrystalline thin films. Unlike the traditional TR spectroscopy, the carrier transport dynamics along the film thickness is resolved by making the pump (excitation) and probe beams spatially separated on each side of the film, so that the carrier transport from the excitation side to the probe side is directly captured. Utilizing this method, the longitudinal carrier diffusion coefficients () in various perovskite films with different thicknesses and grain sizes (extracted from SEM images) are determined, showing values of ∼1.5 to 1.8 cm s (∼0.5 to 0.8 cm s) for films with grain size larger (smaller) than the thickness. This empirical correlation between the longitudinal and film thickness/grain size provides a reference for quick quality screening and evaluation of perovskite polycrystalline thin films.
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http://dx.doi.org/10.1039/d2sc01826hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9297385PMC
July 2022
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