Publications by authors named "Jun Wu"

2,635 Publications

  • Page 1 of 1

Factor analysis of the relationship between PANSS score and family burden of patients with schizophrenia.

Brain Behav 2021 Jun 14. Epub 2021 Jun 14.

Department of Chest and Breast, Qiandongnan People's Hospital.

Objective: This study aims to investigate the burden of family caregivers of patients with schizophrenia, and its influencing factors METHODS: A total of 105 patients with schizophrenia and their caregivers were investigated using the positive and negative symptom scale (PANSS) and family burden scale of disease (FBS) RESULTS: There was a strong correlation between the patient's recovery and family burden, especially between positive and negative symptoms and family financial burden, family daily activities, family recreational activities, and family relationship CONCLUSION: There is a strong correlation between the patient's recovery and family burden, and this is especially correlated to family economic burden, family daily activities, family recreational activities, and family relationship. Medical staff should pay attention to the psychological characteristics of patients and fully understand and avoid the adverse effects of family burden on the rehabilitation of patients.
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http://dx.doi.org/10.1002/brb3.2229DOI Listing
June 2021

Fucoidan Extracted From Sporophyll of Grown in Weihai, China - Chemical Composition and Comparison of Antioxidant Activity of Different Molecular Weight Fractions.

Front Nutr 2021 28;8:636930. Epub 2021 May 28.

Faculty of Health and Environmental Sciences, School of Public Health and Interdisciplinary Studies, Auckland University of Technology, Auckland, New Zealand.

Fucoidan is a multifunctional marine carbohydrate polymer that differs in its chemical composition and bioactivity both between seaweed species and within species from different locations across the globe. In this study, fucoidan was extracted from the sporophyll of grown in Weihai, Shandong Province, China. Fucoidan fractions with molecular weight cutoffs (MWCO) of >300 kDa and <10 kDa were obtained via dialysis. The fucoidan standard from Sigma (Fstd, ≥95, CAS: 9072-19-9), fucoidan crude extract (WH), >300 kDa fraction (300k) and <10 kDa fraction (10k) were compared in terms of chemical composition and antioxidant capacity. Based on Fourier transform infrared spectroscopy (FT-IR) analysis, Fstd, WH, and 300k all showed strong bands around 830 cm, corresponding to the sulfate substituent in the molecule. The results showed that compared with WH and 300 k, the degree of sulfation at 10k was the lowest. From Nuclear magnetic resonance spectroscopy (NMR) result, the four fucoidan samples all contain α--fucose. The primary antioxidant ability of the 10k is significantly higher than that of the 300k, WH, and Fstd, but the secondary antioxidant capabilities of the 10k and 300k were similar, and both were higher than that of the butylated hydroxyanisole (BHA). The ferric reducing antioxidant ability was higher in the 300k and WH fractions. This demonstrates that fucoidan extracted from grown in Weihai, China should be a useful nutraceutical resource.
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http://dx.doi.org/10.3389/fnut.2021.636930DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193228PMC
May 2021

Edible Materials in Tissue Regeneration.

Macromol Biosci 2021 Jun 12:e2100114. Epub 2021 Jun 12.

School of Biomedical Engineering, Sun Yat-sen University, Shenzhen, 518057, China.

Edible materials have attracted increasing attention because of their excellent properties including availability, biocompatibility, biological activity, and biodegradability. Natural polysaccharides, phenolic compounds, and proteins are widely used in tissue regeneration. To better characterize their healing effect, this review article describes the applications of edible materials in tissue regeneration including wound healing and bone tissue regeneration. As an introduction to the topic, their sources and main bioactive properties are discussed. Then, the mechanism by which they facilitate wound healing based on their hemostasis, antibacterial, anti-inflammatory, and antioxidant properties is systematically investigated. Moreover, a more comprehensive discussion is presented on the approaches by which edible materials can be used as scaffolds or agents for the provision of the components of natural bones for regulating the level of osteogenesis-related cytokines to enhance bone repair. Finally, the prospects of edible materials for tissue regeneration are discussed.
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http://dx.doi.org/10.1002/mabi.202100114DOI Listing
June 2021

Dynamic Event-Triggered State Estimation for Markov Jump Neural Networks With Partially Unknown Probabilities.

IEEE Trans Neural Netw Learn Syst 2021 Jun 10;PP. Epub 2021 Jun 10.

This article focuses on the investigation of finite-time dissipative state estimation for Markov jump neural networks. First, in view of the subsistent phenomenon that the state estimator cannot capture the system modes synchronously, the hidden Markov model with partly unknown probabilities is introduced in this article to describe such asynchronization constraint. For the upper limit of network bandwidth and computing resources, a novel dynamic event-triggered transmission mechanism, whose threshold parameter is constructed as an adjustable diagonal matrix, is set between the estimator and the original system to avoid data collision and save energy. Then, with the assistance of Lyapunov techniques, an event-based asynchronous state estimator is designed to ensure that the resulting system is finite-time bounded with a prescribed dissipation performance index. Ultimately, the effectiveness of the proposed estimator design approach combining with a dynamic event-triggered transmission mechanism is demonstrated by a numerical example.
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http://dx.doi.org/10.1109/TNNLS.2021.3085001DOI Listing
June 2021

The Relationship of Morphological-Hemodynamic Characteristics, Inflammation, and Remodeling of Aneurysm Wall in Unruptured Intracranial Aneurysms.

Transl Stroke Res 2021 Jun 9. Epub 2021 Jun 9.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, No.119 South 4th Ring West Road, Fengtai District, Beijing, China.

Aneurysm wall remodeling (AWR) is an important pathological characteristic in aneurysm wall, which was characterized by abnormal histological structure and inflammation infiltration. In the present study, the aim is to determine the relationships of morphological-hemodynamic characteristics, inflammation, and AWR in intracranial aneurysms (IAs), as well as the pathological basis of morphological-hemodynamic predictors to achieve IA development. For this end, 113 unruptured IAs were prospectively collected from 110 cases. In addition, patient-specific computational fluid dynamics and geometry were adopted to determine hemodynamic and morphological parameters. Moreover, Hematoxylin-Eosin staining was performed to identify the AWR. By performing immunofluorescence, the inflammatory markers were detected. Masson staining was conducted to characterize the characteristics of atherosclerosis in aneurysm wall. To demonstrate the parameters regarding the AWR, a multivariate logistic analysis was conducted. Besides, correlation analyses were conducted to verify the relationship between morphological-hemodynamic and pathological characteristics. For 113 unruptured IAs, no difference was identified in baseline information. AWR was demonstrated in 92 (81.4%) IAs. To be specific, the aneurysm size (odds ratio (OR), 2.63; confidence interval (CI), 1.04-6.67; P = 0.041), size ratio (SR; OR, 1.95; CI, 1.38-2.76; P < 0.001), normalized wall shear stress average (NWSSA; OR, 0.05; CI, 0.01-0.15; P = 0.007), and relative resident time (RRT; OR, 1.28; CI, 1.07-1.53; P = 0.007) were proved as the factors of AWR. As revealed from the results of immunofluorescence, aneurysm size, SR, NWSSA, and RRT were significantly correlated with the level of inflammation in IA tissues. Furthermore, Masson staining revealed that atherosclerosis area in IA tissues and NWSSA was correlated with RRT. In this study, SR, NWSSA, and RRT were demonstrated as the risk factors of AWR. The mentioned parameters could also reflect the characteristics of inflammation and atherosclerosis in aneurysm wall as well. This study revealed that biomechanical stress and inflammation in aneurysm wall are correlated, which might suggest the pathological evidence of morphological-hemodynamic predictors for IA development.
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http://dx.doi.org/10.1007/s12975-021-00917-1DOI Listing
June 2021

Effects of Fe(III) and Cu(II) on the sorption of s-triazine herbicides on clay minerals.

J Hazard Mater 2021 Jun 1;418:126232. Epub 2021 Jun 1.

Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, Center for Membrane and Water Science & Technology, Zhejiang University of Technology, Hangzhou 310014, China. Electronic address:

The effects of Fe(III) and Cu(II) on the sorption of atrazine (AT) and prometryn (PY) on clay minerals were investigated both preloaded and in solution. For smectite, Fe(III) preloading greatly enhanced AT and PY sorption at pH 4.0 and 6.0 but diminished AT sorption at pH 8.0. Cu(II) preloading promoted AT and PY sorption under alkaline conditions but suppressed AT sorption at pH 4.0. The adverse effects were not obvious for PY. While for illite and kaolinite, Fe(III) and Cu(II) had little or promotion effects due to the lower contents of them in these two minerals. In the co-sorption studies, for smectite, AT sorption remained at pH 4.0 and increased at pH 6.0 and 8.0, while PY sorption was inhabited over the pH range of 4.0-8.0 in the presence of Fe(III). AT and PY sorption were not affected by Cu(II) except for PY at pH 8.0, in which case, the sorption was promoted. For illite and kaolinite, Fe(III) and Cu(II) generally enhanced AT and PY sorption.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126232DOI Listing
June 2021

Late-stage C(sp)-H and C(sp)-H glycosylation of -aryl/alkyl glycopeptides: mechanistic insights and fluorescence labeling.

Chem Sci 2020 Mar 24;11(25):6521-6526. Epub 2020 Mar 24.

Institut fuer Organische und Biomolekulare Chemie, Georg-August-Universitaet Gottingen Tammannstrasse 2 37077 Goettingen Germany

C(sp)-H and C(sp)-H glycosylations of structurally complex amino acids and peptides were accomplished through the assistance of triazole peptide-isosteres. The palladium-catalyzed peptide-saccharide conjugation provided modular access to structurally complex -alkyl glycoamino acids, glycopeptides and -aryl glycosides, while enabling the assembly of fluorescent-labeled glycoamino acids. The C-H activation approach represents an expedient and efficient strategy for peptide late-stage diversification in a programmable as well as chemo-, regio-, and diastereo-selective fashion.
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http://dx.doi.org/10.1039/d0sc01260bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8152807PMC
March 2020

Circular RNA circDUS2 Is a Potential Biomarker for Intracranial Aneurysm.

Front Aging Neurosci 2021 19;13:632448. Epub 2021 May 19.

Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

CircRNAs have been found to play a crucial role in the pathological process of various kinds of diseases. However, the role of circRNAs in the formation and rupture of intracranial aneurysm is still unknown. Differentially expressed circRNAs profiles between superficial temporal arteries ( = 5) and intracranial aneurysms ( = 5) were analyzed using the Arraystar human circRNAs microarray. Quantitative real-time PCR was utilized to validate the differential expression of circDUS2. Fluorescence hybridization (FISH) was meant for the location of circDUS2 in human brain vascular smooth muscle cell (HBVSMC). Structural analysis was used to speculate on the function of circDUS2. Five hundred forty-three upregulated and 397 downregulated significantly in intracranial aneurysm as compared to superficial temporal arteries. Quantitative real-time PCR verified the elevated expression of the upregulated circDUS2. The FISH test revealed that circDUS2 is located in the cytoplasm of brain vascular smooth muscle cells. This study showed differential expression data of circRNAs between superficial temporal artery and intracranial aneurysm and revealed that circDUS2 is a potential molecular marker for intracranial aneurysm.
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http://dx.doi.org/10.3389/fnagi.2021.632448DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171118PMC
May 2021

High FAS expression correlates with a better prognosis and efficacy of taxanes and target regents in breast cancer.

Cancer Biomark 2021 Jun 5. Epub 2021 Jun 5.

Department of Breast Surgery and Oncology, Key Laboratory of Cancer Prevention and Intervention, National Ministry of Education, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.

Background: FAS can serve as both an oncogene and a suppresser in different malignancies, and the prognostic value of FAS remains controversial.

Methods: The Oncomine database, KM-Plotter and bc-GenExMiner platform were adopted to analyze the prognostic value of FAS in breast cancer. Breast cancer tissue microarrays were further used to verify these data. The Cell Miner Tool was used to predict the value of FAS mRNA expression in predicting the efficacies of clinical drugs.

Results: We found that both FAS mRNA and protein expression level significantly reduced in breast carcinoma. In addition, high FAS expression indicates a better metastatic relapse-free survival. Interestingly, FAS was associated with a better prognosis in different subtypes of breast cancer patients, namely, only in grade II and III, lymph nodal positive or p53 wild-type patients. The data from the Cell Miner Tool revealed that FAS mRNA expression was correlated with the efficacy of the first-line chemotherapeutic taxane agents and target drugs including olaparib and everolimus.

Conclusions: FAS expression correlates with a better prognosis in breast cancer and may provide an effective clinical strategy to predict the sensitivity of taxanes and targeted drugs.
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http://dx.doi.org/10.3233/CBM-203125DOI Listing
June 2021

Age-related defects in autophagy alter the secretion of paracrine factors from bone marrow mononuclear cells.

Aging (Albany NY) 2021 Jun 4;13. Epub 2021 Jun 4.

Toronto General Hospital Research Institute, University Health Network, Toronto, Canada.

Bone marrow mononuclear cell therapy improves cardiac repair after myocardial infarction (MI), in-part through signaling to resident cardiac cells, such as fibroblasts, which regulate scar formation. The efficacy of cell therapy declines with age, as aging of both donor and recipient cells decreases repair responses. Autophagy regulates the microenvironment by both extracellular vesicle (EV)-dependent and independent secretion pathways. We hypothesized that age-related autophagy changes in bone marrow cells (BMCs) alter paracrine signaling, contributing to lower cell therapy efficacy. Here, we demonstrate that young Sca-1 BMCs exhibited a higher LC3II/LC3I ratio compared to old Sca-1 BMCs, which was accentuated when BMCs were cultured under hypoxia. To examine the effect on paracrine signaling, old cardiac fibroblasts were cultured with conditioned medium (CM) from young and old Sca-1 BMCs. Young, but not old CM, enhanced fibroblast proliferation, migration, and differentiation, plus reduced senescence. These beneficial effects were lost when autophagy or EV secretion in BMCs was blocked pharmacologically, or by siRNA knockdown of . Therefore, both EV-dependent and -independent paracrine signaling from young BMCs is responsible for paracrine stimulation of old cardiac fibroblasts. , bone marrow chimerism of old mice with young BMCs increased the number of LC3b cells in the heart compared to old mice reconstituted with old BMCs. These data suggest that the deterioration of autophagy with aging negatively impacts the paracrine effects of BMCs, and provide mechanistic insight into the age-related decline in cell therapy efficacy that could be targeted to improve the function of old donor cells.
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http://dx.doi.org/10.18632/aging.203127DOI Listing
June 2021

Generation of induced pluripotent stem cell line (ZZUi0021-A) from a patient with spinocerebellar ataxia type 19.

Stem Cell Res 2021 May 16;53:102320. Epub 2021 Apr 16.

Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address:

Spinocerebellar ataxia type 19 (SCA19) is an extremely rare autosomal dominant cerebellar ataxia hereditary that caused by the KCND3 gene mutation. And has a complex pathogenesis. At present, its pathogenesis is still unclear, and there is no effective treatment for SCA19. So, to study its pathogenesis and find effective treatments, we collected the fifibroblasts from a patient with SCA19, then successfully transformed the fifibroblasts into induced pluripotent stem cells (iPSCs) and construct a SCA19 pathological cell mode. This study provides a basis for elucidating its pathogenesis and providing new treatment options.
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http://dx.doi.org/10.1016/j.scr.2021.102320DOI Listing
May 2021

Comprehensive DNA methylation-transcriptome profiles association analysis During the Treatment of Acute Myelocytic Leukemia.

Curr Pharm Des 2021 Jun 3. Epub 2021 Jun 3.

Department of Hematology, the Second Affiliated Hospital of Anhui Medical University, Hefei, China.

Background: Epigenetic modifications have recently attracted much attention in the study of the biological mechanisms of acute myelocytic leukemia (AML) for therapy and prognosis. However, studies on DNA methylation changes during AML treatment are limited.

Objective: The comprehensive DNA methylation-transcriptome profiles association analysis in this study aimed to establish whole-genome DNA methylation profiles and explore DNA methylation-related genes and their potential functions before and after treatment. And more appropriate biomarkers are expected to be identified for therapy strategies in AML.

Method: Illumina 450K and RNA-Seq data were obtained from The Cancer Genome Atlas. We performed comprehensive DNA methylation-transcriptome profiles association analysis, pathway analysis, correlation analysis, and survival analyses. The StarBase database was utilized to predict interactions between lncRNAs, miRNAs and target mRNAs.

Results: In total, 1592 distinct CpG sites and 2419 different expression transcripts were identified between pre-treatment and post-treatment AML. The significantly enriched functions of methylated genes were stem cell differentiation, cell population maintenance, and cell development. The expression of UGT3A2, MOG, and VSTM1 was correlated with DNA methylation levels (r2>0.5). Lastly, we identified 4 lncRNAs, 9 miRNAs and 142 mRNAs to construct a lncRNA-miRNA-mRNA ceRNA network.

Conclusion: Our results revealed that DNA methylation was altered before and after treatment. Alterations in DNA methylation affected target gene expression and participated in the key biological processes of AML. Therefore, ceRNA networks may provide further insight into the study of favorable therapeutic markers in AML.
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http://dx.doi.org/10.2174/1381612827666210603165253DOI Listing
June 2021

An electro-spun tri-component polymer biomaterial with optoelectronic properties for neuronal differentiation.

Acta Biomater 2021 May 31. Epub 2021 May 31.

Toronto General Hospital Research Institute, Division of Cardiovascular Surgery, University Health Network, Toronto, Canada; Department of Surgery, Division of Cardiovascular Surgery, University of Toronto, Toronto, Canada. Electronic address:

Optoelectronic biomaterials have recently emerged as a potential treatment option for neurodegenerative diseases, such as optic macular degeneration. Though initial works in the field have involved bulk heterojunctions mimicking solar panels with photovoltaics (PVs) and conductive polymers (CPs), recent developments have considered abandoning CPs in such systems. Here, we developed a simple antioxidant, biocompatible, and fibrous membrane heterojunction composed of photoactive polymer poly(3-hexylthiophene) (P3HT), polycaprolactone (PCL) and polypyrrole (PPY), to facilitate neurogenesis of PC-12 cells when photo-stimulated in vitro. The photoactive prototype, referred to as PCL-P3HT/PPY, was fabricated via polymerization of pyrrole on electro-spun PCL-P3HT nanofibers to form a membrane. Four experimental groups, namely PCL alone, PCL/PPY, PCL-P3HT and PCL-P3HT/PPY, were tested. In the absence of the CP, PCL-P3HT demonstrated lower cell survival due to increased intracellular reactive oxygen/nitrogen species production. PCL-P3HT/PPY rescued these cells by virtue of scavenging radicals, where the CP, PPY, acted as an antioxidant. Apart from having lower impedance, the material also enhanced neurogenesis of PC-12 cells when photo-stimulated, compared to the traditional PCL-P3HT. Lastly, the in vitro system with PC-12 was used to demonstrate the practicality of the material for potential use as a cellular patch in optic and nerve regeneration. This work demonstrated the importance of maintaining PV-CP heterojunctions while simultaneously providing an optoelectrical platform for neural and optical tissue engineering. STATEMENT OF SIGNIFICANCE: Regeneration and repair of injured nervous systems have always been a major clinical challenge. Stem cell therapy is a promising approach for nerve regeneration, and opto-electrical stimulation, which converts light into an electrical signal, has been shown to efficiently regulate stem cell behaviors with enhanced neurogenesis. We developed a micro-fibrous membrane, composed of photoactive polymer, P3HT, scaffold material PCL and conductive polymer PPY. Our heterojunction system improved cell survival via PPY quenching PCL-P3HT-generated cell-damaging reactive oxygen species. PPY also conducted electrons produced from light-stimulated P3HT to promote neurogenesis. This photoactive microfiber biomaterial has great potential as a highly biocompatible and efficient platform to wirelessly promote neurogenesis and survival. Our approach thus showed possibilities with respect to optical tissue engineering.
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http://dx.doi.org/10.1016/j.actbio.2021.05.036DOI Listing
May 2021

GPC5 suppresses lung cancer progression and metastasis via intracellular CTDSP1/AhR/ARNT signaling axis and extracellular exosome secretion.

Oncogene 2021 Jun 2. Epub 2021 Jun 2.

Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, P.R. China.

Lung cancer is the leading cause of cancer-related death worldwide. Glypican-5 (GPC5) is a member of heparan sulfate proteoglycans, and its biological importance in initiation and progression of lung cancer remains controversial. In the present study, we revealed that GPC5 transcriptionally enhanced the expression of CTDSP1 (miR-26b host gene) via AhR-ARNT pathway, and such up-regulation of CTDSP1 intracellularly contributed to the inhibited proliferation of lung cancer cells. Moreover, exosomes derived from GPC5-overexpressing human lung cancer cells (GPC5-OE-derived exosomes) had an extracellular repressive effect on human lymphatic endothelial cells (hLECs), leading to decreased tube formation and migration. Comparison between GPC5-WT- and GPC5-OE-derived exosomes showed that miR-26b (embedded within introns of CTDSP1 gene) was significantly up-regulated in GPC5-OE-derived exosomes and critical to the influence on hLECs. On the mechanism, we demonstrated that miR-26b transferred into hLECs directly targeted to PTK2 3'-UTR and led to PTK2 down-regulation, resulting in defects in tube formation and migration of hLECs. By uncovering the regulation network among GPC5, miR-26b, miR-26b host gene (CTDSP1), and target gene (PTK2), our findings demonstrated that GPC5 functioned as a tumor suppressor in human lung cancer.
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http://dx.doi.org/10.1038/s41388-021-01837-yDOI Listing
June 2021

Impact of 4th of July Fireworks on Spatiotemporal PM Concentrations in California Based on the PurpleAir Sensor Network: Implications for Policy and Environmental Justice.

Int J Environ Res Public Health 2021 05 27;18(11). Epub 2021 May 27.

Program in Public Health, Department of Environmental and Occupational Health, Susan and Henry Samueli College of Health Sciences, University of California, Irvine, CA 92697, USA.

Fireworks are often used in celebration, causing short term, extremely high particulate matter air pollution. In recent years, the rapid development and expansion of low-cost air quality sensors by companies such as PurpleAir has enabled an understanding of air pollution at a much higher spatiotemporal resolution compared to traditional monitoring networks. In this study, real-time PM measurements from 751 PurpleAir sensors operating from June to July in 2019 and 2020 were used to examine the impact of 4th of July fireworks on hourly and daily PM concentrations at the census tract and county levels in California. American Community Survey (ACS) and CalEnviroScreen 3.0 data were used to identify correlations between PM measurements and socioeconomic status (SES). A two-step method was implemented to assure the quality of raw PM sensor data and sensor calibration against co-located reference instruments. The results showed that over 67% and 81% of counties experienced immediate impacts related to fireworks in 2019 and 2020, respectively. Relative to 2019, the peak PM concentrations on July 4th and 5th 2020 were, on average, over 50% higher in California, likely due to the COVID-19-related increase in the use of household-level fireworks. This increase was most pronounced in southern counties, which tend to have less strict firework-related regulations and a greater use of illegal fireworks. Los Angeles County experienced the highest July 4th daily PM levels both in 2019 (29.9 µg·m) and 2020 (42.6 µg·m). Spatial hot spot analyses generally showed these southern counties (e.g., Los Angeles County) to be regional air pollution hotspots, whereas the opposite pattern was seen in the north (e.g., San Francisco). The results also showed PM peaks that were over two-times higher among communities with lower SES, higher minority group populations, and higher asthma rates. Our findings highlight the important role that policy and enforcement can play in reducing firework-related air pollution and protecting public health, as exemplified by southern California, where policy was more relaxed and air pollution was higher (especially in 2020 when the 4th of July coincided with the COVID-19-lockdown period), and in disadvantaged communities where disparities were greatest.
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http://dx.doi.org/10.3390/ijerph18115735DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8198140PMC
May 2021

Short-Term Exposure to Ambient Air Pollution and Increased Emergency Room Visits for Skin Diseases in Beijing, China.

Toxics 2021 May 12;9(5). Epub 2021 May 12.

Department of Occupational and Environmental Health Sciences, School of Public Health, Peking University, Beijing 100191, China.

Skin diseases have become a global concern. This study aims to evaluate the associations between ambient air pollution and emergency room visits for skin diseases under the background of improving air quality in China. Based on 45,094 cases from a general hospital and fixed-site monitoring environmental data from 2014-2019 in Beijing, China, this study used generalized additive models with quasi-Poisson regression to estimate the exposure-health associations at lag 0-1 to lag 0-7. PM and NO exposure were associated with increased emergency room visits for total skin diseases (ICD10: L00-L99). Positive associations of PM, PM, O and NO with dermatitis/eczema (ICD-10: L20-30), as well as SO and NO with urticaria (ICD-10: L50) visits were also found. For instance, a 10 μg/m increase in PM was associated with increases of 0.7% (95%CI: 0.2%, 1.2%) in total skin diseases visits at lag 0-5 and 1.1% (95%CI: 0.6%, 1.7%) in dermatitis/eczema visits at lag 0-1, respectively. For PM, PM and CO, stronger annual associations were typically observed in the high-pollution (2014) and low-pollution (2018/2019) years. For instance, a 10 μg/m increase in PM at lag 0-5 was associated with increases of 1.8% (95%CI: 1.0%, 2.6%) and 2.3% (95%CI: 0.4%, 4.3%) in total skin disease visits in 2014 and 2018, respectively. Our study emphasizes the necessity of controlling the potential health hazard of air pollutants on skin, although significant achievements in air quality control have been made in China.
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http://dx.doi.org/10.3390/toxics9050108DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8151157PMC
May 2021

Expression of Antigens Fused to Different Vectors and Their Effectiveness as Vaccines.

Vaccines (Basel) 2021 May 21;9(6). Epub 2021 May 21.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, 20 Dongdajie Street, Fengtai District, Beijing 100071, China.

Pertussis is an acute respiratory tract infection caused by . Even though its current vaccine coverage is relatively broad, they still have some shortcomings such as short protection time and might be incapable of blocking the spread of the disease. In this study, we developed new pertussis vaccine candidates by separately fusing three pertussis antigens ( fimbriae 2 "Fim2", pertussis toxin S1 subunit "PtxS1", and filamentous hemagglutinin "FHA") to each of two immune-boosting carrier proteins (B subunits of AB5 toxin family: cholera toxin B subunit "CTB" and shiga toxin B subunit "StxB"). We then immunized mice with these fusion antigens and found that they significantly increased the serum antibody titers and elicited high bactericidal activity against . After CTB-or StxB-fused antigen-immunized mice were challenged with a non-lethal dose of , the bacterial loads in different tissues of these mice were significantly reduced, and their lung damage was nearly invisible. Furthermore, we also demonstrated that these candidate vaccines could provide strong prophylactic effects against a lethal challenge with . Overall, our candidate vaccines conferred better immune protection to mice compared with pertussis antigen alone. This B5 subunit-based vaccine strategy provides a promising option for vaccine design.
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http://dx.doi.org/10.3390/vaccines9060542DOI Listing
May 2021

Abdominal paracentesis drainage attenuates intestinal mucosal barrier damage through macrophage polarization in severe acute pancreatitis.

Exp Biol Med (Maywood) 2021 May 30:15353702211015144. Epub 2021 May 30.

College of Medicine, Southwest Jiaotong University, Chengdu 610031, China.

Abdominal paracentesis drainage (APD), as an effective treatment of severe acute pancreatitis (SAP) in clinical settings, can ameliorate intestinal barrier damage and the overall severity of SAP. However, the mechanism underlying therapeutic effects of APD on damaged intestinal mucosal barrier during SAP is still unclear. Here, SAP was induced by injecting 5% Na-taurocholate retrograde into the biliopancreatic duct of rats to confirm the benefits of APD on enteral injury of SAP and further explore the possible mechanism. Abdominal catheter was placed after SAP was induced in APD group. As control group, the sham group received no operation except abdominal opening and closure. By comparing changes among control group, sham group, and APD group, APD treatment obviously lowered the intestinal damage and reduced the permeation of intestinal mucosal barrier, which was evidenced by intestinal H&E staining, enteral expression of tight junction proteins, intestinal apoptosis measurement and detection of serum diamine oxidase, intestinal fatty acid binding protein and D-lactic acid. Furthermore, we found that APD polarized intestinal macrophages toward M2 phenotype by the determination of immunofluorescence and western blotting, and this accounts for the benefits of APD for intestinal injury in SAP. Importantly, the protective effect against intestinal injury by APD treatment was mediated through the inhibited ASK1/JNK pathway. In summary, APD improved the intestinal mucosal barrier damage in rats with SAP through an increasing portion of M2 phenotype macrophages in intestine via inhibiting ASK1/JNK pathway.
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http://dx.doi.org/10.1177/15353702211015144DOI Listing
May 2021

Modifications of polysaccharide-based biomaterials under structure-property relationship for biomedical applications.

Carbohydr Polym 2021 Aug 24;266:118097. Epub 2021 Apr 24.

Guangxi Engineering Center in Biomedical Materials for Tissue and Organ Regeneration, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China. Electronic address:

Polysaccharides are well accepted biomaterials that have attracted considerable attention. Compared with other materials under research, polysaccharides show unique advantages: they are available in nature and are normally easily acquired, those acquired from nature show favorable immunogenicity, and are biodegradable and bioavailable. The bioactivity and possible applications are based on their chemical structure; however, naturally acquired polysaccharides sometimes have unwanted flaws that limit further applications. For this reason, carefully summarizing the possible modifications of polysaccharides to improve them is crucial. Structural modifications can not only provide polysaccharides with additional functional groups but also change their physicochemical properties. This review based on the structure-property relation summarizes the common chemical modifications of polysaccharides, the related bioactivity changes, possible functionalization methods, and major possible biomedical applications based on modified polysaccharides.
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http://dx.doi.org/10.1016/j.carbpol.2021.118097DOI Listing
August 2021

A global metagenomic map of urban microbiomes and antimicrobial resistance.

Cell 2021 May 22. Epub 2021 May 22.

Weill Cornell Medicine, New York, NY, USA; The Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, New York, NY, USA.

We present a global atlas of 4,728 metagenomic samples from mass-transit systems in 60 cities over 3 years, representing the first systematic, worldwide catalog of the urban microbial ecosystem. This atlas provides an annotated, geospatial profile of microbial strains, functional characteristics, antimicrobial resistance (AMR) markers, and genetic elements, including 10,928 viruses, 1,302 bacteria, 2 archaea, and 838,532 CRISPR arrays not found in reference databases. We identified 4,246 known species of urban microorganisms and a consistent set of 31 species found in 97% of samples that were distinct from human commensal organisms. Profiles of AMR genes varied widely in type and density across cities. Cities showed distinct microbial taxonomic signatures that were driven by climate and geographic differences. These results constitute a high-resolution global metagenomic atlas that enables discovery of organisms and genes, highlights potential public health and forensic applications, and provides a culture-independent view of AMR burden in cities.
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http://dx.doi.org/10.1016/j.cell.2021.05.002DOI Listing
May 2021

Simplification of culture conditions and feeder-free expansion of bovine embryonic stem cells.

Sci Rep 2021 May 26;11(1):11045. Epub 2021 May 26.

Department of Animal Science, University of California, 450 Bioletti Way, Davis, CA, 95616, USA.

Bovine embryonic stem cells (bESCs) extend the lifespan of the transient pluripotent bovine inner cell mass in vitro. After years of research, derivation of stable bESCs was only recently reported. Although successful, bESC culture relies on complex culture conditions that require a custom-made base medium and mouse embryonic fibroblasts (MEF) feeders, limiting the widespread use of bESCs. We report here simplified bESC culture conditions based on replacing custom base medium with a commercially available alternative and eliminating the need for MEF feeders by using a chemically-defined substrate. bESC lines were cultured and derived using a base medium consisting of N2B27 supplements and 1% BSA (NBFR-bESCs). Newly derived bESC lines were easy to establish, simple to propagate and stable after long-term culture. These cells expressed pluripotency markers and actively proliferated for more than 35 passages while maintaining normal karyotype and the ability to differentiate into derivatives of all three germ lineages in embryoid bodies and teratomas. In addition, NBFR-bESCs grew for multiple passages in a feeder-free culture system based on vitronectin and Activin A medium supplementation while maintaining pluripotency. Simplified conditions will facilitate the use of bESCs for gene editing applications and pluripotency and lineage commitment studies.
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http://dx.doi.org/10.1038/s41598-021-90422-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8155104PMC
May 2021

Production of a Promising Biosynthetic Self-Assembled Nanoconjugate Vaccine against Klebsiella Pneumoniae Serotype O2 in a General Escherichia Coli Host.

Adv Sci (Weinh) 2021 May 24:e2100549. Epub 2021 May 24.

State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, No. 20, Dongda Street, Fengtai District, Beijing, 100071, P. R. China.

Klebsiella pneumoniae has emerged as a severe opportunistic pathogen with multiple drug resistances. Finding effective vaccines against this pathogen is urgent. Although O-polysaccharides (OPS) of K. pneumoniae are suitable antigens for the preparation of vaccines given their low levels of diversity, the low immunogenicity (especially serotype O2) limit their application. In this study, a general Escherichia coli host system is developed to produce a nanoscale conjugate vaccine against K. pneumoniae using the Nano-B5 self-assembly platform. The experimental data illustrate that this nanoconjugate vaccine can induce an efficient humoral immune response in draining lymph nodes (dLNs) and elicit high titers of the IgG antibody against bacterial lipopolysaccharide (LPS). The ideal prophylactic effects of these nanoconjugate vaccines are further demonstrated in mouse models of both systemic and pulmonary infection. These results demonstrate that OPS with low immunogenicity can be changed into an effective antigen, indicating that other haptens may be applicable to this strategy in the future. To the knowledge, this is the first study to produce biosynthetic nanoconjugate vaccines against K. pneumoniae in E. coli, and this strategy can be applied to the development of other vaccines against pathogenic bacteria.
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http://dx.doi.org/10.1002/advs.202100549DOI Listing
May 2021

Natural Killer Cells Regulate the Maturation of Liver Sinusoidal Endothelial Cells Thereby Promoting Intrahepatic T-Cell Responses in a Mouse Model.

Hepatol Commun 2021 May 5;5(5):865-881. Epub 2021 Feb 5.

Department of Infectious Diseases Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China.

Functional maturation of liver sinusoidal endothelial cells (LSECs) plays an important role in intrahepatic T-cell activation and control of viral infections. Natural killer (NK) cells have been reported to prompt the maturation of antigen-presenting cells (APCs), especially for dendritic cells (DCs), but the interaction between NK cells and LSECs is elusive. Here, we investigated whether and how NK cells are involved in regulating LSEC maturation and if this has a role in controlling hepatitis B virus (HBV) infection in a mouse model. A chronic HBV replication mouse model was established by hydrodynamic injection (HI) of 6 µg adeno-associated virus plasmid (pAAV)/HBV 1.2. The nucleotide-binding oligomerization domain-containing protein 1 (NOD1) ligand diaminopemelic acid (DAP) was imported into liver by HI at day 14 after plasmid injection. We found that HI of DAP recruited conventional NK cells (cNK) into the liver and promoted tumor necrosis factor alpha (TNF-α) and interferon-gamma (IFN-γ) production of NK cells in a chemokine (C-X-C motif) receptor 3 (CXCR3)-dependent manner. Importantly, the maturation of LSECs and the anti-HBV effects of DAP were impaired in CXCR3 mice; this possibly was associated with the decreased number of intrahepatic cNK cells. Consistently, depleting cNK cells but not liver-resident NK cells also impaired the maturation and antigen-presenting function of LSECs, which reduced intrahepatic HBV-specific T-cell responses and thus inhibited HBV clearance both in wild-type and in Rag1 mice. Moreover, TNF-α or IFN-γ stimulation as well as coculture with intrahepatic NK cells partly promoted LSEC phenotypic and functional maturation . NOD1-triggered NK cell activation may lead to the enhancement of intrahepatic T-cell responses by promoting maturation of LSECs through soluble cytokines and cell-cell contact, thereby controlling HBV replication and expression.
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http://dx.doi.org/10.1002/hep4.1676DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8122378PMC
May 2021

A Fifteen-Gene Classifier to Predict Neoadjuvant Chemotherapy Responses in Patients with Stage IB to IIB Squamous Cervical Cancer.

Adv Sci (Weinh) 2021 05 18;8(10):2001978. Epub 2021 Mar 18.

Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Jiefang Avenue 1095# Wuhan Hubei 430030 China.

Neoadjuvant chemotherapy (NACT) remains an attractive alternative for controlling locally advanced cervical cancer. However, approximately 15-34% of women do not respond to induction therapy. To develop a risk stratification tool, 56 patients with stage IB-IIB cervical cancer are included in 2 research centers from the discovery cohort. Patient-specific somatic mutations led to NACT non-responsiveness are identified by whole-exome sequencing. Next, CRISPR/Cas9-based library screenings are performed based on these genes to confirm their biological contribution to drug resistance. A 15-gene classifier is developed by generalized linear regression analysis combined with the logistic regression model. In an independent validation cohort of 102 patients, the classifier showed good predictive ability with an area under the curve of 0.80 (95% confidence interval (CI), 0.69-0.91). Furthermore, the 15-gene classifier is significantly associated with patient responsiveness to NACT in both univariate (odds ratio, 10.8; 95% CI, 3.55-32.86; = 2.8 × 10) and multivariate analysis (odds ratio, 17.34; 95% CI, 4.04-74.40; = 1.23 × 10) in the validation set. In conclusion, the 15-gene classifier can accurately predict the clinical response to NACT before treatment, representing a promising approach for guiding the selection of appropriate treatment strategies for locally advanced cervical cancer.
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http://dx.doi.org/10.1002/advs.202001978DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132153PMC
May 2021

The impact of neutrophil extracellular traps on deep venous thrombosis in patients with traumatic fractures.

Clin Chim Acta 2021 Aug 18;519:231-238. Epub 2021 May 18.

Department of Laboratory Medicine, Beijing Jishuitan Hospital, Peking University Fourth School of Clinical Medicine, Beijing, China; Department of Laboratory Medicine, Beijing Jishuitan Hospital, Beijing, China. Electronic address:

Background: Deep venous thrombosis (DVT) is the most common complication in patients with traumatic fractures. The neutrophil extracellular traps (NETs) can promote thrombus formation. In this prospective study, we investigated the role of NETs in thrombosis in patients with traumatic fractures to evaluate whether the biomarkers of NETs can be used to help predict the risk of thrombosis.

Methods: Traumatic fracture patients were enrolled in this prospective observational cohort study. Healthy controls (Control); patients with lower extremity fractures who neither present with nor develop DVT (Trauma non-DVT); patients with lower extremity fractures who do not present with DVT but do develop DVT (Trauma DVT); and patients with lower extremity fractures who present with DVT (DVT) were included. NETs biomarker levels of Citrullinated Histone H3 (H3Cit), cell-free DNA (cfDNA) and nucleosomes in the plasma were determined. The D-dimer and fibrin(-ogen) degradation products (FDP) level in plasma was measured. Statistical analysis of the test results was performed to assess changes in NETs biomarker levels during thrombosis in patients with traumatic fractures.

Results: The H3Cit levels in the DVT group were significantly greater than in the Trauma non-DVT group and Trauma DVT group (1.88(1.11, 3.35) ng/ml Vs 0.38(0.10, 1.17) ng/ml, P ≤ 0.05). The level of cfDNA was significantly greater in patients with traumatic fractures and was higher after thrombosis than before. The levels of D-dimer in the DVT, Trauma DVT, and Trauma non-DVT groups were significantly greater than in the Control group (5.11(3.97, 8.11) mg/l; 6.12(2.59, 18.49) mg/l; 2.99(0.99, 9.02) mg/l Vs 0.18(0.08,0.24) mg/l, P < 0.05). The distribution of FDP levels in each group was similar to that of D-dimer. Data are presented as medians (25th percentile, 75th percentile).

Conclusions: NETs released by neutrophils are involved in the formation of DVTs in patients with traumatic fractures. H3Cit and cfDNA can assist the diagnosis of DVT in patients with traumatic fractures.
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http://dx.doi.org/10.1016/j.cca.2021.04.021DOI Listing
August 2021

Anti-inflammation biomaterial platforms for chronic wound healing.

Biomater Sci 2021 Jun;9(12):4388-4409

Key Laboratory of Sensing Technology and Biomedical Instrument of Guangdong Province, School of Biomedical Engineering, Sun Yat-sen University, Shenzhen 518107, P. R. China.

Nowadays, there has been an increase in the number of people with chronic wounds, which has resulted in serious health problems worldwide. The rate-limiting stage of chronic wound healing has been found to be the inflammation stage, and strategies for shortening the prolonged inflammatory response have proven to be effective for increasing the healing rate. Recently, various anti-inflammatory strategies (such as anti-inflammatory drugs, antioxidant, NO regulation, antibacterial, immune regulation and angiogenesis) have attracted attention as potential therapeutic pathways. Moreover, various biomaterial platforms based on anti-inflammation therapy strategies have also emerged in the spotlight as potential therapies to accelerate the repair of chronic wounds. In this review, we systematically investigated the advances of various biomaterial platforms based on anti-inflammation strategies for chronic wound healing, to provide valuable guidance for future breakthroughs in chronic wound treatment.
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http://dx.doi.org/10.1039/d1bm00637aDOI Listing
June 2021

TRPM7 kinase-mediated immunomodulation in macrophage plays a central role in magnesium ion-induced bone regeneration.

Nat Commun 2021 05 17;12(1):2885. Epub 2021 May 17.

Department of Orthopaedics and Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.

Despite the widespread observations on the osteogenic effects of magnesium ion (Mg), the diverse roles of Mg during bone healing have not been systematically dissected. Here, we reveal a previously unknown, biphasic mode of action of Mg in bone repair. During the early inflammation phase, Mg contributes to an upregulated expression of transient receptor potential cation channel member 7 (TRPM7), and a TRPM7-dependent influx of Mg in the monocyte-macrophage lineage, resulting in the cleavage and nuclear accumulation of TRPM7-cleaved kinase fragments (M7CKs). This then triggers the phosphorylation of Histone H3 at serine 10, in a TRPM7-dependent manner at the promoters of inflammatory cytokines, leading to the formation of a pro-osteogenic immune microenvironment. In the later remodeling phase, however, the continued exposure of Mg not only lead to the over-activation of NF-κB signaling in macrophages and increased number of osteoclastic-like cells but also decelerates bone maturation through the suppression of hydroxyapatite precipitation. Thus, the negative effects of Mg on osteogenesis can override the initial pro-osteogenic benefits of Mg. Taken together, this study establishes a paradigm shift in the understanding of the diverse and multifaceted roles of Mg in bone healing.
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http://dx.doi.org/10.1038/s41467-021-23005-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128914PMC
May 2021

Human Position Brace Versus Pavlik Harness for Infants Under 6 Months of Age With Developmental Dislocation of the Hip: A Comparison of Therapeutic Efficacy.

J Pediatr Orthop 2021 May 13. Epub 2021 May 13.

Department of Orthopedic, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University, Chongqing, China.

Background: For 0- to 6-month-old patients with developmental dislocation of the hip, the likelihood of Pavlik harness treatment failure may be increased. The human position brace is an available option. We aimed to compare therapeutic efficacy between the Pavlik harness and human position brace.

Methods: We included 87 patients (106 hips) in the human position brace group and 47 patients (62 hips) in the Pavlik harness group. Clinical and radiologic outcomes were recorded and compared, respectively.

Results: The mean follow-up was 17.73±14.56 months (range, 5 to 56 mo). Human position brace produced higher success rates (65.1% vs. 43.5%; P=0.006) and had significant advantages in the 4 to 6 months group (P<0.001). According to the last follow-up x-ray, when compared with that of the Pavlik harness group, upper space was larger (P=0.032) and inner space was smaller (P<0.001) in the human position brace group.

Conclusions: Given the increased success rates and better hip development by the last follow-up, the human position brace could be considered as an alternative, or even as the first choice for 4- to 6-month-old infants with developmental dislocation of the hip to address the specific deficits of Pavlik harness.

Level Of Evidence: Level III-retrospective comparison.
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http://dx.doi.org/10.1097/BPO.0000000000001862DOI Listing
May 2021

Elucidating the effects of TiO nanoparticles on the toxicity and accumulation of Cu in soybean plants (Glycine max L.).

Ecotoxicol Environ Saf 2021 Aug 11;219:112312. Epub 2021 May 11.

College of Environmental Sciences, Sichuan Agricultural University, Chengdu 611130, PR China. Electronic address:

Copper (Cu) pollution is common in the soil. Due to the widespread application of TiO NPs, there is a high propensity for the co-occurrence of TiO nanoparticles (NPs) and Cu in agricultural soils. It is therefore imperative to evaluate the joint effects of TiO NPs and Cu on crops. In this study, the mutual effects of TiO NPs and Cu on their toxicity and accumulation in soybean seedlings and on their fates in a hydroponic system were determined. When Cu was at levels of 1 and 2 mg/L, the co-occurring TiO NPs at a non-toxic concentration (10 mg/L) significantly enhanced the toxicity and accumulation of Cu and Ti in soybeans, and inhibited the translocation of Cu from soybean roots to shoots. However, when the Cu concentration for co-exposure was ≥ 5 mg/L, such mutual effects disappeared. The amount of Cu ions adsorbed onto TiO NPs after 48 h of co-exposure gradually increased from 31 to 118 mg/g when the Cu concentration was increased from 1 to 20 mg/L. The aggregation and sedimentation of TiO NPs were significantly increased after 48 h of co-exposure with the Cu at a concentration higher than 5 mg/L, as compared to the single TiO NPs exposure. The increasing aggregation and sedimentation might reduce the bioavailability of TiO NPs associated with the adsorbed Cu to soybeans, and consequently alleviate or even neutralize the enhanced toxicity and accumulation of Cu in soybeans exerted by the co-existing TiO NPs. Our results thus suggest that consideration of the impact of TiO NPs on the phytotoxicity of heavy metals, and specifically Cu, needs to be interpreted with care, and highlight the importance of integrating the interaction and fates of TiO NPs and metals into their risk assessment.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112312DOI Listing
August 2021

Identification of potential genomic biomarkers for Parkinson's disease using data pooling of gene expression microarrays.

Biomark Med 2021 Jun 14;15(8):585-595. Epub 2021 May 14.

Department of Neurology, Peking University Shenzhen Hospital, Shenzhen, 518036, PR China.

In this study, we aimed to identify potential diagnostic biomarkers Parkinson's disease (PD) by exploring microarray gene expression data of PD patients. Differentially expressed genes associated with PD were screened from the GSE99039 dataset using weighted gene co-expression network analysis, followed by gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses, gene-gene interaction network analysis and receiver operator characteristics analysis. We identified two PD-associated modules, in which genes from the chemokine signaling pathway were primarily enriched. In particular, , ,  and directly interacted with known PD-associated genes and showed higher expression in the PD samples, and may thus be potential biomarkers in PD diagnosis. A DFG-analysis identified a four-gene panel (, , , ) as a potential diagnostic predictor to diagnose PD.
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http://dx.doi.org/10.2217/bmm-2020-0325DOI Listing
June 2021