Publications by authors named "Jun Tang"

970 Publications

Realize High Thermoelectric Properties in n-Type BiTeSe/YO Nanocomposites by Constructing Heterointerfaces.

ACS Appl Mater Interfaces 2021 Aug 4. Epub 2021 Aug 4.

College of Physics, Sichuan University, Chengdu 610065, China.

Due to the excellent thermoelectric performance, bismuth telluride (BiTe) compounds are highly promising for the thermoelectric conversion in the room temperature range. However, the inferior thermoelectric performance of the n-type leg severely restricts the applications of BiTe-based thermoelectric couples. Herein, n-type BiTeSe (BTS)-based thermoelectric materials incorporated with nanosized YO (0.5-3 wt %) are prepared and their thermoelectric properties are systematically studied. The dramatically improved thermoelectric performance is ascribed to the realization of a multiscale feature of YO nanoparticle (NP)-induced interfacial decorations distributed along grain boundaries, which creates massive BTS/YO interfaces for the manipulation of carrier and phonon transport properties. The geometric phase analysis is employed to further confirm the condition of local strain in the BTS composite incorporated with YO NPs. Due to the presence of heterointerfaces and high density of dislocations in BTS matrices, the minimum lattice thermal conductivity (κ) of the nanocomposites (NCs) is dramatically suppressed from 0.76 to 0.37 W m K. With the incorporation of 3 wt % YO NPs, the Vickers hardness of the BTS/YO NC is increased by about 32%. Overall, the BTS + 1.5 wt % YO NC maintains excellent thermoelectric properties (ZT = 1.1) in the whole operative temperature range (300-500 K). The present strategy of implementing high-density heterogeneous interfaces by YO NP addition offers an applicable pathway for fabricating high-performance thermoelectric materials with both optimized thermoelectric properties and mechanical properties.
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http://dx.doi.org/10.1021/acsami.1c12722DOI Listing
August 2021

Polymorphism analysis and expression patterns of the gene in the Shitou goose.

Arch Anim Breed 2021 22;64(2):315-323. Epub 2021 Jul 22.

College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, P.R. China.

Insulin-like growth factor 1 () is one of the endocrine hormones that plays an important role in regulating growth and development of animals. In this study, polymorphism in the 5 UTR and 3 UTR coding region and of the gene was detected by DNA sequencing technology, and the abundance of mRNA in various tissues at three growth stages of the Shitou goose was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Moreover, the differential expression of in various tissues between the Shitou goose and Wuzong goose was revealed. Two single nucleotide polymorphisms (SNPs) were found in the exon3 region of in the Shitou goose. mRNA was extensively expressed in various tissues of Shitou geese with high abundant expression in the liver, breast muscle and leg muscle at three growth stages. mRNA expression showed a trend of first increase and then decrease in the pituitary, liver, subcutaneous fat and abdominal fat tissues, but it decreased in the breast muscle and leg muscle of a Shitou goose with growing age. Expression of in the liver, leg muscle and pituitary tissues of the Shitou goose was significantly higher than that of the Wuzong goose. This provides a foundation for further study of regulatory mechanisms of in the growth and development of geese.
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http://dx.doi.org/10.5194/aab-64-315-2021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8320522PMC
July 2021

Substrate-Independent TiCT MXene Waterborne Paint for Terahertz Absorption and Shielding.

ACS Nano 2021 Aug 2. Epub 2021 Aug 2.

School of Electronic Science and Engineering, State Key Laboratory of Electronic Thin Film and Integrated Devices, University of Electronic Science and Technology of China, Chengdu, Sichuan 610054, PR China.

With the development of terahertz (THz) technology, there is a booming demand of THz shielding/absorption materials to avoid electromagnetic interference (EMI) or pollution. Paints that can be fast solidified to form a film and stably adherent on arbitrary substrates are especially desired for the shielding/absorption applications. Recently, MXenes with high electron conductivity and hydrophilicity have attracted a great interest for EMI shielding. Here, we demonstrate a copolymer-polyacrylic latex (PAL) based MXene waterborne paint (MWP), which not only has strong THz EMI shielding/absorption efficiency but also can easily adhere onto various substrates that are commonly used in the THz band. The viscosity of MWP can be tuned by adjusting the colloidal and viscous forces, and the cyano group in PAL provides a strong intermolecular polar interaction between MWP and the substrate. As a result, a 38.3-μm-thick MWP on quartz exhibits EMI shielding value of 64.9 dB, and an excellent reflection-loss of 32.8 dB is obtained on MWP coated sponge foam. This substrate-independent MWP provides a simple and efficient way to achieving high-performance THz shielding/absorption.
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http://dx.doi.org/10.1021/acsnano.1c04656DOI Listing
August 2021

Glucose oxidase and FeO/TiO/AgPO co-embedded biomimetic mineralization hydrogels as controllable ROS generators for accelerating diabetic wound healing.

J Mater Chem B 2021 Jul 26. Epub 2021 Jul 26.

Department of Polymer Science, College of Chemistry, Jilin University, Changchun 130012, China.

The hyperglycemic environment and the presence of bacterial infections delay the healing of diabetic wounds. Herein, glucose oxidase (GOx) and Fe3O4/TiO2/Ag3PO4 were embedded in a polyacrylic acid-calcium phosphate ([email protected]@GOx) hydrogel through an in situ biomimetic mineralization approach. The GOx encapsulation efficiency was 96.75% and exhibited exceptional enzyme activity stability. Moreover, the co-immobilization of GOx and Fe3O4/TiO2/Ag3PO4 nanoparticles generated a simple and multifunctional antibacterial platform with the advantages of decreasing blood glucose concentration and efficiently producing reactive oxygen species (ROS). In addition, the degradation rate of the hydrogel was controlled by regulating the concentration of phosphate thus controlling the release of Fe3O4/TiO2/Ag3PO4 and GOx. As a result, both the potential toxicity and oxidative stress associated with the antimicrobial biomaterial can be controlled within the body therefore potentially preventing detriment. In vivo results indicated that the [email protected]@GOx hydrogel effectively promoted diabetic wound healing and showed great potential for clinical applications of chronic wound management.
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http://dx.doi.org/10.1039/d1tb00711dDOI Listing
July 2021

RNA demethylation increases the yield and biomass of rice and potato plants in field trials.

Nat Biotechnol 2021 Jul 22. Epub 2021 Jul 22.

Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, China.

RNA N-methyladenosine (mA) modifications are essential in plants. Here, we show that transgenic expression of the human RNA demethylase FTO in rice caused a more than threefold increase in grain yield under greenhouse conditions. In field trials, transgenic expression of FTO in rice and potato caused ~50% increases in yield and biomass. We demonstrate that the presence of FTO stimulates root meristem cell proliferation and tiller bud formation and promotes photosynthetic efficiency and drought tolerance but has no effect on mature cell size, shoot meristem cell proliferation, root diameter, plant height or ploidy. FTO mediates substantial mA demethylation (around 7% of demethylation in poly(A) RNA and around 35% decrease of mA in non-ribosomal nuclear RNA) in plant RNA, inducing chromatin openness and transcriptional activation. Therefore, modulation of plant RNA mA methylation is a promising strategy to dramatically improve plant growth and crop yield.
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http://dx.doi.org/10.1038/s41587-021-00982-9DOI Listing
July 2021

Cancer cells escape p53's tumor suppression through ablation of ZDHHC1-mediated p53 palmitoylation.

Oncogene 2021 Jul 19. Epub 2021 Jul 19.

Chongqing Key Laboratory of Molecular Oncology and Epigenetics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.

The inactivation of tumor-suppressor genes contributes heavily to oncogenesis. The mutation of TP53 has been well-studied and recognized as a major factor in the development of tumors. Yet other means of p53 inactivation has not been well-elucidated. We previously identified a hypermethylated gene ZDHHC1 that suppresses tumor growth when the expression was restored, but the specific mechanism was yet to be found. The protein product of ZDHHC1 is an S-palmitoyltransferase and we have identified p53 as a substrate for ZDHHC1-mediated palmitoylation, specifically at the C135, C176, and C275 residues. The novel form of post-translational modification of p53 is required for the nuclear translocation of the tumor suppressor. p53 recruited DNMT3A to ZDHHC1 promoter and is responsible for the hypermethylation of ZDHHC1. The epigenetic feedback loop formed by ZDHHC1 and p53 sheds light on the inactivation of p53 without the presence of genetic mutations.
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http://dx.doi.org/10.1038/s41388-021-01949-5DOI Listing
July 2021

Pseudorabies Virus DNA Polymerase Processivity Factor UL42 Inhibits Type I IFN Response by Preventing ISGF3-ISRE Interaction.

J Immunol 2021 Jul 16. Epub 2021 Jul 16.

College of Veterinary Medicine, China Agricultural University, Beijing, China; and

Alphaherpesviruses are large dsDNA viruses with an ability to establish persistent infection in hosts, which rely partly on their ability to evade host innate immune responses, notably the type I IFN response. However, the relevant molecular mechanisms are not well understood. In this study, we report the UL42 proteins of alphaherpesvirus pseudorabies virus (PRV) and HSV type 1 (HSV1) as a potent antagonist of the IFN-I-induced JAK-STAT signaling pathway. We found that ectopic expression of UL42 in porcine macrophage CRL and human HeLa cells significantly suppresses IFN-α-mediated activation of the IFN-stimulated response element (ISRE), leading to a decreased transcription and expression of IFN-stimulated genes (ISGs). Mechanistically, UL42 directly interacts with ISRE and interferes with ISG factor 3 (ISGF3) from binding to ISRE for efficient gene transcription, and four conserved DNA-binding sites of UL42 are required for this interaction. The substitution of these DNA-binding sites with alanines results in reduced ISRE-binding ability of UL42 and impairs for PRV to evade the IFN response. Knockdown of UL42 in PRV remarkably attenuates the antagonism of virus to IFN in porcine kidney PK15 cells. Our results indicate that the UL42 protein of alphaherpesviruses possesses the ability to suppress IFN-I signaling by preventing the association of ISGF3 and ISRE, thereby contributing to immune evasion. This finding reveals UL42 as a potential antiviral target.
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http://dx.doi.org/10.4049/jimmunol.2001306DOI Listing
July 2021

Tumor targeting nanoparticle E7-HSP110-RGD elicits potent anti-tumor immune response in a CD8-dependent manner in cervical cancer-bearing mouse model.

Hum Vaccin Immunother 2021 Jul 16:1-10. Epub 2021 Jul 16.

Department of Dermatology, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.

Our previous research verified that HSP (heat shock protein) 110 could enhance the anti-tumor effect of HPV16 E7 epitope. In this study, to optimize the immunotherapy of this vaccine type, we developed and evaluated the anti-tumor immunity of a nanoparticle vaccine format assembling with E7-HSP110 fusion expression plasmid and RGD-GGG-K polypeptide. The nanoparticle vaccine was self-assembled from positively charged RGD-GGG-K polypeptide and negatively charged fusion expression plasmid pIRES2-3× E7-HSP110-EGFP. The particle size, stability, expression of E7-HSP110 fusion protein and the target ability of nanoparticle were determined, respectively. Specific CTL responses were determined by E7 tetramer staining and cytotoxicity assay in TC-1 tumor-bearing mice (CD4/CD8 knockout). The preventive and therapeutic experiments of nanoparticle vaccine were investigated in TC-1 tumor-bearing mice. Results showed that the RGD-GGG-K polypeptide and pIRES2-3× E7-HSP110-EGFP plasmid self-assembled nanoparticles about 100 nanometers in diameter when the charge ratios of peptide/plasmid were 2. The nanoparticles effectively entered TC-1 cells directed by RGD target-peptide, and correctly expressed the E7-HSP110 fusion protein. The HSP110 effectively facilitated nanoparticles activating CD8T cells than nanoparticles without HSP110, including the CD8 T cell number and the IFN-γ level; in contrast, the CD4T cells immune response remained indiscriminate among the mice groups. This nanoparticle formulation inhibited tumor growth and prolonged the survival duration in the prophylactic and therapeutic mouse models. Therefore, the RGD-based tumor-targeting nanoparticle expressing E7-HSP110 fusion protein can efficiently evoke anti-tumor activity and thus suggests it might be a favorable candidate for cervical cancer immunotherapy.
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http://dx.doi.org/10.1080/21645515.2021.1933875DOI Listing
July 2021

Panicle Apical Abortion 3 Controls Panicle Development and Seed Size in Rice.

Rice (N Y) 2021 Jul 15;14(1):68. Epub 2021 Jul 15.

Rice Research Institute, Key Laboratory of Application and Safety Control of Genetically Modified Crops, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.

Background: In rice, panicle apical abortion is a common phenomenon that usually results in a decreased number of branches and grains per panicle, and consequently a reduced grain yield. A better understanding of the molecular mechanism of panicle abortion is thus critical for maintaining and increasing rice production.

Results: We reported a new rice mutant panicle apical abortion 3 (paa3), which exhibited severe abortion of spikelet development on the upper part of the branches as well as decreased grain size over the whole panicle. Using mapping-based clone, the PAA3 was characterized as the LOC_ Os04g56160 gene, encoding an H-ATPase. The PAA3 was expressed highly in the stem and panicle, and its protein was localized in the plasma membrane. Our data further showed that PAA3 played an important role in maintaining normal panicle development by participating in the removal of reactive oxygen species (ROS) in rice.

Conclusions: Our studies suggested that PAA3 might function to remove ROS, the accumulation of which leads to programmed cell death, and ultimately panicle apical abortion and decreased seed size in the paa3 panicle.
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http://dx.doi.org/10.1186/s12284-021-00509-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8282854PMC
July 2021

PET Imaging of Translocator Protein as a Marker of Malaria-Associated Lung Inflammation.

Infect Immun 2021 Jul 12:IAI0002421. Epub 2021 Jul 12.

Singapore Immunology Network, A* STAR, 8A Biomedical Grove, Immunos, Singapore, 138648.

Malaria-associated acute respiratory distress syndrome (MA-ARDS) is a severe complication of malaria despite effective anti-malarial treatment. Currently, non-invasive imaging procedures such as chest X-rays are used to assess oedema in established MA-ARDS but earlier detection methods are needed to reduce morbidity and mortality. The early stages of MA-ARDS are characterized by the infiltration of leukocytes, in particular monocyte/macrophages, thus monitoring of immune infiltrates may provide a useful indicator of early pathology. ANKA-infected C57BL/6 mice, a rodent malaria model of MA-ARDS, were longitudinally imaged using the TSPO imaging agent [F]FEPPA as a marker of macrophage accumulation during the development of pathology and response to combined artesunate and chloroquine diphosphate therapy (ART+CQ). [F]FEPPA uptake was compared to blood parasitemia levels and pulmonary immune cell infiltrates using flow cytometry. Infected animals showed rapid increases lung retention of [F]FEPPA, correlating well with increases in blood parasitemia and pulmonary accumulation of interstitial inflammatory macrophages and MHC II+ alveolar macrophages. Treatment with ART+CQ therapy abrogated this increase in parasitemia and significantly reduced both lung uptake of [F]FEPPA and macrophage infiltrates. Retention of [F]FEPPA in the lungs is well correlated with changes in blood parasitemia and lung associated macrophages during disease progression and in response to ART+CQ therapy. With further development TSPO biomarkers may have the potential to be able to accurately assess early onset of MA-ARDS.
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http://dx.doi.org/10.1128/IAI.00024-21DOI Listing
July 2021

Mass spectrometry-based metabolomics to reveal chicken meat improvements by medium-chain monoglycerides supplementation: Taste, fresh meat quality, and composition.

Food Chem 2021 Jun 5;365:130303. Epub 2021 Jun 5.

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.

This study was conducted to reveal the differences of chicken fresh meat quality, composition and taste induced by medium-chain monoglycerides (MG) supplementation. Results demonstrated that both chicken broth and meat taste were improved by MG supplementation. The up-regulated l-carnosine, sarcosine, uridine and nicotinamide in the chicken broth of the MG group contributed to the umami and meaty taste. Dietary MG increased the total superoxide dismutase activity and amino acid content in the muscle of chicken breast and reduced the malondialdehyde content and drip loss. Moreover, meat metabolome revealed that creatine, betaine, l-anserine, inosine 5'-monophosphate, hypoxanthine, inosine and phospholipid, as well as amino acid and purine metabolism pathway connected to the improvements of meat quality, composition and taste of broilers by MG addition. In conclusion, these findings provide convincing evidence regarding the improvements of fresh meat quality, composition and taste of broilers by MG supplementation.
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http://dx.doi.org/10.1016/j.foodchem.2021.130303DOI Listing
June 2021

Jahn-Teller Distortion Induced Mn-Rich Cathode Enables Optimal Flexible Aqueous High-Voltage Zn-Mn Batteries.

Adv Sci (Weinh) 2021 Jun 5;8(12):2004995. Epub 2021 May 5.

School of Materials Science & Engineering Key Laboratory of Mobile Materials MOE Jilin University Changchun 130012 China.

Although one of the most promising aqueous batteries, all Zn-Mn systems suffer from Zn dendrites and the low-capacity Mn/Mn process (readily leading to the occurrence of Jahn-Teller distortion, which in turn causes structural collapse and voltage/capacity fading). Here, the Mn reconstruction and disproportionation are exploited to prepare the stable, Mn-rich manganese oxides on carbon-cloth (CMOs) in a discharged state through an inverted design, which promotes reversible Mn/Mn kinetics and mitigates oxygen-related redox activity. Such a 1.65 V Mn-rich cathode enable constructing a 2.2 V Zn-Mn battery, providing a high area capacity of 4.16 mA h cm (25 mA h cm for 10 mL electrolyte) and superior 4000-cycle stability. Moreover, a flexible hybrid 2.7 V Zn-Mn battery is constructed using 2-pH hydrogel electrolytes to demonstrate excellent practicality and stability. A further insight has been gained to the commercial application of aqueous energy storage devices toward low-cost, high safety, and excellent energy density.
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http://dx.doi.org/10.1002/advs.202004995DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8224442PMC
June 2021

circPTPN22 as a novel biomarker and ceRNA in peripheral blood mononuclear cells of rheumatoid arthritis.

Mol Med Rep 2021 Aug 29;24(2). Epub 2021 Jun 29.

Dermatology Department of The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, P.R. China.

The role of circular RNAs (circRNAs) in rheumatoid arthritis (RA) remains to be elucidated. To determine the expression of circRNAs in peripheral blood mononuclear cells (PBMCs) and to identify novel biomarkers for RA and explore their potential effects in RA, the present study conducted high‑throughput RNA sequencing to analyze circRNA expression profiles in PBMCs from 4 RA patients and 3 healthy controls (HCs). Reverse transcription‑quantitative PCR was used to verify the expression of circPTPN22 in 42 RA patients, 44 HCs and 45 systemic lupus erythematosus (SLE) patients. In addition, bioinformatics analysis and Pearson's correlation test were conducted to assess the correlation of the relationships between circPTPN22 and RA progression. A receiver operating characteristic curve was calculated to evaluate the diagnostic value. Multilevel integrated analysis identified 41 upregulated and 30 downregulated circRNAs in RA patients compared with HCs. circPTPN22 was confirmed to be a common differentially expressed gene in RA and SLE compared with HCs. Area under the curve analysis suggested the diagnostic value of circPTPN22 expression to distinguish RA patients from both HCs and SLE patients. In addition, circPTPN22 levels in RA PBMCs were correlated with RA‑IgG, RA‑IgM, RA‑IgA, anti‑cyclic citrullinated peptide (anti‑CCP), rheumatoid factor and C reactive protein levels. A total of four putative microRNAs (miRNAs or miRs), namely, hsa‑miR‑3074‑5p, hsa‑miR‑373‑3p, hsa‑miR‑766‑3p and hsa‑miR‑34c‑5p, were screened to be sponged by circPTPN22 via bioinformatics analysis and then experimentally verified to be upregulated in RA PBMCs compared with controls. The data suggested that circPTPN22 might be a novel biomarker for the diagnosis of RA and participate in RA pathogenesis through a sponge mechanism.
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http://dx.doi.org/10.3892/mmr.2021.12256DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8258470PMC
August 2021

miR-211-5p inhibits the proliferation, migration, invasion, and induces apoptosis of human hypertrophic scar fibroblasts by regulating TGFβR2 expression.

Ann Transl Med 2021 May;9(10):864

Department of Plastic and Burn Surgery, West China Hospital, Sichuan University, Chengdu, China.

Background: Hypertrophic-scar (HS) is the most common pathological healing phenomenon after trauma, especially after deep burns. We aimed to investigate the expression and role of microRNA-211-5p (miR-211-5p) in HS and explore its underlying mechanism.

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-211-5p in 15 cases of HS tissues and normal skin tissues, as well as its expression in human hypertrophic scar fibroblasts (hHSFs) and normal fibroblasts. At the same time, the cell counting kit-8 (CCK-8), scratch test, cell invasion test, and flow cytometry were used to determine cell proliferation, migration, invasion, and apoptosis, respectively. Western blot assay was used to determine the expression of proteins. TargetScan was performed to predict the potential binding sites between miR-211-5p and TGFβR2, which was then verified by western blotting and luciferase reporter gene experiments. Also, co-transfection of plasmids that overexpress miR-211-5p and TGFβR2 were used to observe the reversal effect of miR-211-5p.

Results: The level of miR-211-5p in HS tissues and hHSFs cells was significantly down-regulated (both P<0.05). The TGFβR2/Smad3 signaling pathway was activated (both P<0.05). Furthermore, the overexpression of miR-211-5p could inhibit the proliferation (P<0.05), migration (P<0.05), and invasion (P<0.05) of hHSFs cells, and induce their apoptosis (P<0.05), and could also regulate the expression of related proteins (all P<0.05). Moreover, the overexpression of miR-211-5p could also inhibit the accumulation of ECM and the activation of the TGF-βR2/Smad3 pathway (all P<0.05), while the opposite effect (all P<0.05) was observed when the level of miR-211-5p was interfered with. Finally, it was confirmed that miR-211-5p could target TGFβR2 (all P<0.05), and when hHSFs cells simultaneously overexpressed miR-211-5p and TGFβR2, the promotion effect of TGFβR2 on cells was reversed by miR-211-5p (all P<0.05).

Conclusions: miR-211-5p can inhibit the activation of the TGF-βR2/Smad3 signaling pathway by targeting TGFβR2, thereby suppressing the proliferation, migration, invasion, and ECM production of hHSFs, and inducing their apoptosis, suggesting that miR-211-5p can become a potential target for the treatment of HS.
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http://dx.doi.org/10.21037/atm-21-1806DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184471PMC
May 2021

A Ferroptosis-Related lncRNAs Signature Predicts Prognosis and Immune Microenvironment for Breast Cancer.

Front Mol Biosci 2021 7;8:678877. Epub 2021 Jun 7.

Department of Breast Oncology, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-Sen University Cancer Center, Guangzhou, China.

Ferroptosis, a regulated cell death which is driven by the iron-dependent peroxidation of lipids, plays an important role in cancer. However, studies about ferroptosis-related Long non-coding RNAs (lncRNAs) in breast cancer (BC) are limited. Besides, the prognostic role of ferroptosis-related lncRNAs and their relationship to immune microenvironment in breast cancer remain unclear. This study aimed to explore the potential prognostic value of ferroptosis-related lncRNAs and their relationship to immune microenvironment in breast cancer. RNA-sequencing data of female breast cancer patients were downloaded from TCGA database. 937 patients were randomly separated into training or validation cohort in 2:1 ratio. Ferroptosis-related lncRNAs were screened by Pearson correlation analysis with 239 reported ferroptosis-related genes. A ferroptosis-related lncRNAs signature was constructed with univariate and multivariate Cox regression analyses in the training cohort, and its prognostic value was further tested in the validation cohort. An 8-ferroptosis-related-lncRNAs signature was developed by multivariate Cox regression analysis to divide patients into two risk groups. Patients in the high-risk group had worse prognosis than patients in the low-risk group. Multivariate Cox regression analysis showed the risk score was an independent prognostic indicator. Receiver operating characteristic curve (ROC) analysis proved the predictive accuracy of the signature. The area under time-dependent ROC curve (AUC) reached 0.853 at 1 year, 0.802 at 2 years, 0.740 at 5 years in the training cohort and 0.791 at 1 year, 0.778 at 2 years, 0.722 at 5 years in the validation cohort. Further analysis demonstrated that immune-related pathways were significantly enriched in the high-risk group. Analysis of the immune cell infiltration landscape showed that breast cancer in the high-risk group tended be immunologically "cold". We identified a novel ferroptosis-related lncRNA signature which could precisely predict the prognosis of breast cancer patients. Ferroptosis-related lncRNAs may have a potential role in the process of anti-tumor immunity and serve as therapeutic targets for breast cancer.
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http://dx.doi.org/10.3389/fmolb.2021.678877DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215711PMC
June 2021

Integrated multi-omics profiling of high-grade estrogen receptor-positive, HER2-negative breast cancer.

Mol Oncol 2021 Jun 19. Epub 2021 Jun 19.

Department of Endocrine and Breast Surgery, The First Affiliated hospital of Chongqing Medical University, Chongqing Medical University, China.

Estrogen receptor-positive and human epidermal growth factor receptor 2-negative (ER HER2 ) breast cancer accounts for ~ 60-70% of all cases of invasive breast carcinoma. High-grade ER HER2 tumors respond poorly to endocrine therapy. In this study, we systematically analyzed clinical and multi-omics data to find potential strategies for personalized therapy of patients with high-grade ER HER2 disease. Six different cohorts were analyzed, for which multi-omics data were available. Grade III ER HER2 cases harbored higher proportions of large tumor size (> 5 cm), lymph node metastasis, chemotherapy use, and luminal B subtypes defined by PAM50, as compared with grade I/II tumors. DNA methylation (HM450) data and methylation-specific PCR indicated that the cg18629132 locus in the MKI67 promoter was hypermethylated in grade I/II cases and normal tissue, but hypomethylated in grade III cases or triple-negative breast cancer, resulting in higher expression of MKI67. Mutations in ESR1 and TP53 were detected in post-endocrine treatment metastatic samples at a higher rate than in treatment-naive tumors in grade III cases. We identified 42 and 20 focal copy number events in nonmetastatic and metastatic high-grade ER HER2 cases, respectively, with either MYC or MDM2 amplification representing an independent prognostic event in grade III cases. Transcriptional profiling within grade III tumors highlighted ER signaling downregulation and upregulation of immune-related pathways in non-luminal-like tumors defined by PAM50. Recursive partitioning analysis was employed to construct a decision tree of an endocrine-resistant subgroup (GATA3-negative and AGR-negative) of two genes that was validated by immunohistochemistry in a Chinese cohort. All together, these data suggest that grade III ER HER2 tumors have distinct clinical and molecular characteristics compared with low-grade tumors, particularly in cases with non-luminal-like biology. Due to the dismal prognosis in this group, clinical trials are warranted to test the efficacy of potential novel therapies.
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http://dx.doi.org/10.1002/1878-0261.13043DOI Listing
June 2021

Non-invasive SERS serum detection technology combined with multivariate statistical algorithm for simultaneous screening of cervical cancer and breast cancer.

Anal Bioanal Chem 2021 Aug 14;413(19):4775-4784. Epub 2021 Jun 14.

First Affiliated Hospital of Xinjiang Medical University, Xinjiang, 830046, Urumqi, China.

Surface-enhanced Raman scattering (SERS), as a rapid, reliable and non-destructive spectral detection technology, has made a series of breakthrough achievements in screening and pre-diagnosis of various cancerous tumors. In this paper, high-performance gold nanoparticles/785 porous silicon photonic crystals (Au NPs/785 PSi PhCs) active SERS substrates were specially designed for serum testing, and realized highly sensitive detection of serum from healthy people, patients with cervical cancer and breast cancer. Based on the SERS spectra of the three groups of serum, the significant differences between the healthy group and cancer group at 1030 cm and 1051 cm were analyzed, and the similar but different serum SERS spectra of cervical cancer and breast cancer patients were compared. In addition, the spectral difference detected by SERS technology combined with a multivariate statistical algorithm was used to distinguish three kinds of serum. The serum SERS spectral sensitive bands were extracted by recursive weighted partial least squares (rPLS), and the three classification diagnosis models were established by combining orthogonal partial least squares discriminant analysis (OPLS-DA), linear discriminant analysis (LDA) and principal component analysis support vector machine (PCA-SVM) for synchronous classification and discrimination of the three groups of serum. The diagnostic results showed that the overall screening accuracy of three models were 93.28%, 97.77% and 94.78%, respectively. These above results confirmed that the Au NPs/785 PSi PhCs can realize super-sensitive detection of serum, and the established diagnostic model has great potential for pre-diagnosis and simultaneous screening of cervical cancer and breast cancer.
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http://dx.doi.org/10.1007/s00216-021-03431-3DOI Listing
August 2021

Dissecting the multi-omics atlas of the exosomes released by human lung adenocarcinoma stem-like cells.

NPJ Genom Med 2021 Jun 14;6(1):48. Epub 2021 Jun 14.

Translational Medicine Collaborative Innovation Center, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong, China.

Lung adenocarcinoma is heterogeneous and hierarchically organized, with a subpopulation of stem-like cells (CSCs) that reside at the apex of the hierarchy, in which exosomes act as important mediators by transporting specific molecules among different cell populations. Although there have been numerous studies on tumor exosomes, the constituents and functional properties of CSC-derived exosomes are still poorly characterized. Here we present a detail transcriptome and proteome atlas of the exosomes released by human lung adenocarcinoma stem-like cells (LSLCs). The transcriptome analysis indicates the specific patterns of exosomal constituents, including the fragmentation of transcripts and the low-level presence of circular RNAs, and identifies multiple exosomal-enriched mRNAs and lncRNAs. Integrative analysis of transcriptome and proteome data reveals the diverse functions of exosomal-enriched RNAs and proteins, many of which are associated with tumorigenesis. Importantly, several LSLC markers we identified are highly expressed in LSLC-derived exosomes and associate with poor survival, which may serve as promising liquid biopsy biomarkers for lung adenocarcinoma diagnosis. Our study provides a resource for the future elucidation of the functions of tumor-derived exosomes and their regulatory mechanisms in mediating lung cancer development.
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http://dx.doi.org/10.1038/s41525-021-00217-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203745PMC
June 2021

Stabilizing Triglyceride in Methanol Emulsions via a Magnetic Pickering Interfacial Catalyst for Efficient Transesterification under Static Conditions.

ACS Omega 2021 Jun 24;6(22):14138-14147. Epub 2021 May 24.

State Key Laboratory Breeding Base of Green Chemistry-Synthesis Technology, Zhejiang Province Key Laboratory of Biofuel, Biodiesel Laboratory of China Petroleum and Chemical Industry Federation, College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, P. R. China.

Pickering emulsion systems provide potential platforms for simultaneously intensifying and catalyzing transesterification between triglyceride and methanol under static conditions. However, realizing static transesterification with high biodiesel yield is still challenging due to low emulsion stability at the reaction temperature. Here, a series of magnetically recyclable Pickering interfacial catalysts (PICs) with similar surface affinities but different densities were constructed as stabilizers of a soybean oil/methanol emulsion. The variations in the emulsion volume fraction and droplet size were comparatively studied and analyzed from the viewpoint of droplet settling and catalyst particle shedding. It is found that, except for surface affinity, PIC density also plays a pivotal role in emulsion stability owing to the non-negligible effect of gravity on catalyst adsorption in triglyceride/methanol emulsion (especially at elevated temperature). By reducing the density, finely improving the lipophilicity, and optimizing the addition amount of PIC, the obtained soybean oil/methanol emulsion can remain stable for at least 12 h at 60 °C, enabling static transesterification with a high biodiesel yield of 95.6%. Moreover, the best performing PIC can be reused for at least 7 cycles. This efficient static transesterification system offers a green strategy for biodiesel production.
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http://dx.doi.org/10.1021/acsomega.1c00629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8190790PMC
June 2021

A Novel Canine Mammary Cancer Cell Line: Preliminary Identification and Utilization for Drug Screening Studies.

Front Vet Sci 2021 27;8:665906. Epub 2021 May 27.

College of Veterinary Medicine, China Agricultural University, Beijing, China.

Canine malignant mammary tumor is a dangerously fatal neoplastic disease with poor survival in female dogs. The aim of this study was to preliminary characterize a novel canine mammary cancer cell line, B-CMT, from canine primary mammary gland tumor, and to utilize it as a cell model for screening of possible therapeutic drugs. The successfully established cell line, B-CMT, was cultured over 50 passages. B-CMT has a fast proliferation rate, and a population doubling time (PDT) of 33.6 h. The B-CMT cell line lacked human epidermal growth factor receptor-2 (HER-2), estrogen receptors (ER) and progesterone receptors (PR) expression by qRT-PCR. Compared with MDCK cells, CDH1 expression of CMT cell line was significantly decreased or even absent, but GATA3 expression dramatically increased, while TGF-β expression was at a similar level. Interestingly, the B-CMT cell line from canine primary tumor also showed positive hypoxia inducible factor-1α (HIF-1α) results in immunofluorescence (IF), western blot, and qRT-PCR analysis. Ten days post inoculation with EGFP-B-CMT (B-CMT cells stably expressing EGFP), the experimental mice developed palpable soft tissue masses which histologically resembled the canine primary tumor, and was approved to be derived from B-CMT cell line through detection of EGFP by immunohistochemical (IHC) analysis. Moreover, we investigated the cytotoxicity of five drugs to B-CMT cells, and the results showed that rapamycin and imatinib significantly inhibited the proliferation of the cells within a certain range of concentration. They also induced cell cycle arrest of B-CMT cells at G1 and G2 phase, respectively. In summary, the results of this report showed that B-CMT cell line might serve as a tool for future studies on tumor microenvironment and drug resistance.
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http://dx.doi.org/10.3389/fvets.2021.665906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191460PMC
May 2021

Melatonin Is a Promising Silage Additive: Evidence From Microbiota and Metabolites.

Front Microbiol 2021 26;12:670764. Epub 2021 May 26.

Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, China.

The safe and effective storage of forage are very important. As an important storage method, ensiling can keep fresh forage for a long time with less nutritional loss. Melatonin has antioxidant and bacteriostasis, usually used as a natural preservative. The influence of melatonin on silage microbial or fermentation quality has not been clarified. In the present study, we aimed to clarify whether melatonin affected stylo () silage quality via microbiota and metabolites. Melatonin addition significantly improved the silage fermentation quality, including the increased contents of lactic acid and total acid (244.18-255.81% and 63.95-78.97%, respectively), as well as the decreased in pH and butyric acid content compare with control group. Moreover, 16S rRNA sequencing indicated that melatonin addition enhanced the silage microbial diversity indices (such as increase in Shannon indices but decrease in Simpson indices), and significantly shaped the composition of silage microbiota (such as increased abundances of , , , and , and decreased abundance of ). Melatonin addition also dramatically affected the metabolites of sylo silage, such as raised malonic acid and some amino acid metabolism(glycine, threonine, methionine and ornithine), while reduced nucleic acid metabolism(2-deoxyuridine and thymine) and carbon metabolism(allose and 2-deoxy-D-glucose). Collectively, our results confirmed that the lowest melatonin addition (5 mg/kg) could improve the fermentation quality, and the potential mechanisms might be associated with the microbiota and metabolites in stylo.
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http://dx.doi.org/10.3389/fmicb.2021.670764DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8187806PMC
May 2021

Dioscorea zingiberensis ameliorates diabetic nephropathy by inhibiting NLRP3 inflammasome and curbing the expression of p66Shc in high-fat diet/streptozotocin-induced diabetic mice.

J Pharm Pharmacol 2021 Jun 1. Epub 2021 Jun 1.

School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing, People's Republic of China.

Objectives: Diabetic nephropathy (DN) is a severe diabetic complication. Dioscorea zingiberensis (DZ) possesses excellent pharmacological properties with lower toxicity. The purpose of this study was to investigate the efficacy and mechanism of DZ in DN.

Methods: DN was established by the high-fat diet combining intraperitoneal injection of streptozotocin in mice. The DZ (125 and 250 mg/kg/day) were intragastrical administered for 8 consecutive weeks. After treatment, blood, urine and kidney tissue were collected for biological detection, renal morphology, fibrosis and molecular mechanism research, respectively.

Key Findings: This study has shown that DZ significantly ameliorated kidney hypertrophy, renal structural damage and abnormal function of the kidney indicators (creatinine, urinary protein and blood urea nitrogen). Further molecular mechanism data suggested that the NLRP3/Cleaved-caspase-1 signal pathway was remarkably activated in DN, and DZ treatment reversed these changes, which indicated that it effectively attenuated inflammatory response caused by hyperglycaemia. In addition, DN inhibits hyperglycaemia-induced activation of oxidative stress by suppressing the expression of p66Shc proteins.

Conclusions: DZ could efficiently suppress oxidative stress and inflammatory responses to postpone the development of DN, and its mechanism might be related to inhibition of NLRP3 and p66Shc activities. Thus, DZ could be developed into a new therapeutic agent for DN.
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http://dx.doi.org/10.1093/jpp/rgab053DOI Listing
June 2021

Establishment of a reliable scheme for obtaining highly stable SERS signal of biological serum.

Biosens Bioelectron 2021 Oct 18;189:113315. Epub 2021 May 18.

First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830046, China. Electronic address:

As a rapid and non-destructive biological serum detection method, SERS technology was widely used in the screening and medical diagnosis of various diseases by combining the analysis of serum SERS spectrum and multivariate statistical algorithm. Because of the high complexity of serum components and the variability of SERS spectra, which often resulted in the phenomenon that the SERS spectrum of the same biological serum was significantly different due to the different test conditions. In this experiment, through the dilution treatment of the serum and the systematic test of the serum of all concentration gradients with lasers of wavelength of 785, 633 and 532 nm, the most suitable conditions for detecting the serum were investigated. The experimental results showed that only when the serum is diluted to low concentration (10 ppm), the SERS spectrum with high reproducibility and stability could be obtained, furthermore, the low concentration serum had weak tolerance to laser, and 532 nm laser was not suitable for serum detection. In this paper, a set of test scheme for obtaining highly stable serum SERS spectra was established by using high-performance gold nanoparticles (Au NPs) as the active substrate of SERS. Through comparative analysis of SERS spectrum of serum of normal people and cervical cancer, the reliability of the established low-concentration serum test program was verified, as well as its great potential advantages in disease screening and diagnosis.
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http://dx.doi.org/10.1016/j.bios.2021.113315DOI Listing
October 2021

Identification of cumin and fennel from different regions based on generative adversarial networks and near infrared spectroscopy.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Nov 13;260:119956. Epub 2021 May 13.

College of Software, Xinjiang University, Urumqi 830046, Xinjiang, China; Key Laboratory of Signal Detection and Processing, Xinjiang University, Urumqi 830046, Xinjiang, China. Electronic address:

Cumin (Cuminum cyminum) and fennel (Foeniculum vulgare) are widely used seasonings and play a very important role in industries such as breeding, cosmetics, winemaking, drug discovery, and nano-synthetic materials. However, studies have shown that cumin and fennel from different regions not only differ greatly in the content of lipids, phenols and proteins but also the substances contained in their essential oils are also different. Therefore, realizing precise identification of cumin and fennel from different regions will greatly help in quality control, market fraud and production industrialization. In this experiment, cumin and fennel samples were collected from each region, a total of 480 NIR spectra were collected. We used deep learning and traditional machine learning algorithms combined with near infrared (NIR) spectroscopy to identify their origin. To obtain the model with the best generalization performance and classification accuracy, we used principal component analysis (PCA) to reduce spectral data dimensionality after Rubberband baseline correction, and then established classification models including quadratic discriminant analysis based on PCA (PCA-QDA) and multilayer perceptron based on PCA (PCA-MLP). We also directly input the spectral data after baseline correction into convolutional neural networks (CNN) and generative adversarial networks (GAN). The experimental results show that GAN is more accurate than the PCA-QDA, PCA-MLP and CNN models, and the classification accuracy reached 100%. In the cumin and fennel classification experiment in the same region, the four models achieve great classification results from three regions under the condition that all model parameters remain unchanged. The experimental results show that when the training data are limited and the dimension is high, the model obtained by GAN using competitive learning has more generalization ability and higher classification accuracy. It also provides a new method for solving the problem of limited training data in food research and medical diagnosis in the future.
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http://dx.doi.org/10.1016/j.saa.2021.119956DOI Listing
November 2021

Does Transient Receptor Potential Vanilloid Type 1 Alleviate or Aggravate Pathological Myocardial Hypertrophy?

Front Pharmacol 2021 10;12:681286. Epub 2021 May 10.

The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, China.

Transient receptor potential vanilloid type 1 (TRPV1) is a non-selective cation channel, which is involved in the endogenous stress adaptation mechanism for protection of the heart as well as the occurrence and development of some heart diseases. Although the effect of activation of the TRPV1 channel on different types of non-neural cells in the heart remains unclear, most data show that stimulation of sensory nerves expressing TRPV1 or stimulation/overexpression of the TRPV1 channel has a beneficial role in heart disease. Some studies have proven that TRPV1 has an important relationship with pathological myocardial hypertrophy, but the specific mechanism and effect are not clear. In order to help researchers better understand the relationship between TRPV1 and pathological myocardial hypertrophy, this paper aims to summarize the effect of TRPV1 and the related mechanism in the occurrence and development of pathological myocardial hypertrophy from the following three points of view: 1) role of TRPV1 in alleviation of pathological myocardial hypertrophy; 2) role of TRPV1 in aggravation of pathological myocardial hypertrophy; and 3) the point of view of our team of researchers. It is expected that new therapies can provide potential targets for pathological myocardial hypertrophy.
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http://dx.doi.org/10.3389/fphar.2021.681286DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8143375PMC
May 2021

Pretreatment with Propofol Reduces Pulmonary Injury in a Pig Model of Intestinal Ischemia-Reperfusion via Suppressing the High-Mobility Group Box 1 Protein (HMGB1)/Toll-Like Receptor 4 (TLR4)/Protein Kinase R (PKR) Signaling Pathway.

Med Sci Monit 2021 May 19;27:e930478. Epub 2021 May 19.

Department of Anesthesiology, Fifth People's Hospital, Fudan University, Shanghai, China (mainland).

BACKGROUND Propofol improves rodent pulmonary injury after intestinal ischemia-reperfusion (IIR). However, its effect and underlying mechanisms in large animals remain unclear. Here, we examined whether pretreatment with propofol could relieve lung injury during IIR in pigs, then investigated the underlying mechanism. MATERIAL AND METHODS A porcine model of IIR-induced lung injury was built by clamping the super mesenteric artery for 2 h and loosening the clamp for 4 h. Randomized grouping was used, and pigs were assigned to a sham-operated group, an IIR with saline pretreatment group, and an IIR with propofol pretreatment group. Pulmonary histopathologic changes, permeability, and oxygenation were assessed to evaluate the effect of propofol. We assessed levels of methane dicarboxylic aldehyde (MDA), superoxide dismutase (SOD), myeloperoxidase (MPO), high-mobility group box 1 protein (HMGB1), Toll-like receptor 4 (TLR4), and double-stranded RNA activated protein kinase R (PKR) to investigate the underlying mechanism. RESULTS IIR caused severe lung damage, including morphological changes, high permeability, airway resistance, low static compliance, hypoxemia, and acidemia. Pulmonary and plasma MDA content and MPO activity increased, whereas SOD activity decreased. The HMGB1/TLR4/PKR signaling pathway was activated following IIR. Pretreatment with propofol markedly attenuated lung injury (such as reducing the lung edema and permeability), increased MDA content and MPO activity, and restored SOD activity induced by IIR, accompanied by inhibiting the effect of the HMGB1/TLR4/PKR signaling pathway. CONCLUSIONS IIR caused acute lung injury in pigs. Pretreatment with propofol alleviated the lung injury, which was related to its suppression of the HMGB1/TLR4/PKR signaling pathway.
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http://dx.doi.org/10.12659/MSM.930478DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8142706PMC
May 2021

Improved thermal multiple-relaxation-time lattice Boltzmann model for liquid-vapor phase change.

Phys Rev E 2021 Apr;103(4-1):043308

School of Energy Science and Engineering, Central South University, Changsha 410083, China.

In this paper, an improved thermal multiple-relaxation-time (MRT) lattice Boltzmann (LB) model is proposed for simulating liquid-vapor phase change. A temperature equation is first derived for liquid-vapor phase change, where the latent heat of vaporization is decoupled with the equation of state. Therefore, the latent heat of vaporization can be arbitrarily specified in practice, which significantly improves the flexibility of the present LB model for liquid-vapor phase change. The Laplacian term of temperature is avoided in the proposed temperature equation and the gradient term of temperature is calculated through a local scheme. To solve the temperature equation accurately and efficiently, an improved MRT LB equation with nondiagonal relaxation matrix is developed. The implicit calculation of the temperature, caused by the source term and encountered in previous works, is avoided by approximating the source term with its value at the previous time step. As demonstrated by numerical tests, the results by the present LB model agree well with analytical results, experimental results, or the results by the finite difference method where the fourth-order Runge-Kutta method is employed to implement the discretization of time.
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http://dx.doi.org/10.1103/PhysRevE.103.043308DOI Listing
April 2021

N-methyladenosine (mA) methyltransferase KIAA1429 accelerates the gefitinib resistance of non-small-cell lung cancer.

Cell Death Discov 2021 May 17;7(1):108. Epub 2021 May 17.

Department of Thoracic Surgery, Cancer Hospital of China Medical University, Shenyang, Liaoning Province, 110042, China.

N-methyladenosine (mA) modification has been convincingly identified to be a critical regulator in human cancer. However, the contribution of mA to NSCLC gefitinib resistance is still largely unknown. Here, we screened and identified that mA methyltransferase KIAA1429 was highly expressed in gefitinib-resistant NSCLC cells (PC9-GR), tissues, and closely related to unfavorable survival. Functionally, KIAA1429 accelerated the gefitinib resistance of NSCLC in vitro. Depletion of KIAA1429 repressed the tumor growth of PC9-GR cells in vivo. Mechanistically, KIAA1429 enhanced the mRNA stability of HOXA1 through targeting its 3'-untranslated regions (3'-UTR). Overall, our findings indicate that KIAA1429 plays essential oncogenic roles in NSCLC gefitinib resistance, which may provide a feasible therapeutic target for NSCLC.
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http://dx.doi.org/10.1038/s41420-021-00488-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128911PMC
May 2021

Screening of the college students at clinical high risk for psychosis in China: a multicenter epidemiological study.

BMC Psychiatry 2021 05 17;21(1):253. Epub 2021 May 17.

Department of Psychiatry, Tongji Hospital of Tongji University, Tongji University School of Medicine, 389 Xincun Road, Shanghai, 200065, PR China.

Background: To investigate a 3-stage screening procedure and explore the clinical features of subjects at Clinical High Risk (CHR) for psychosis in a representative sample of Chinese college students.

Methods: An epidemiological survey of the prevalence of the CHR syndrome in Chinese college students that was selected by stratified random sampling from Shanghai, Nanjing and Nanchang cities was done following a 3-stage procedure. Participants were initially screened with the Prodromal Questionnaire-brief version (PQ-B), and whose distress score of PQ-B exceeded 24 would be invited to a telephone assessment using the subscale for positive symptoms of the Scale of Prodromal Symptoms (SOPS)/Structured Interview for Prodromal Syndromes (SIPS). Lastly, participants who scored 3 or higher in any item of the subscale would be administered with the SIPS interview conducted by trained researchers to confirm the diagnosis of CHR syndrome.

Results: Twenty-three thousand sixty-three college students completed the survey during September 2017 to October 2018. Seventy-two students were diagnosed as CHR subjects, and the detection rate in the total sample was 0.3%. The peak age range for the first diagnosis of CHR was 17 to 20 years. Thirteen and forty-six were set as the cutoff points of PQ-B total score and distress score to balance the greatest sensitivity and specificity. Binary logistic regression revealed that 8 items in PQ-B showed significant distinction for detecting CHR subjects.

Conclusions: The 3-stage screening method can be utilized in the detection of CHR subjects for psychosis in the general population, during which delusional ideas, perceptual abnormalities and suspiciousness deserve great attention.
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http://dx.doi.org/10.1186/s12888-021-03229-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127262PMC
May 2021

Neoantigen landscape in metastatic nasopharyngeal carcinoma.

Theranostics 2021 19;11(13):6427-6444. Epub 2021 Apr 19.

Department of Nasopharyngeal Carcinoma, Sun Yat-sen University Cancer Center, 651 Dongfeng East Road, Guangzhou 510060, P. R. China.

Reportedly, nasopharyngeal carcinoma (NPC) patients with MHC I Class aberration are prone to poor survival outcomes, which indicates that the deficiency of tumor neoantigens might represent a mechanism of immune surveillance escape in NPC. To clearly delineate the landscape of neoantigens in NPC, we performed DNA and RNA sequencing on paired primary tumor, regional lymph node metastasis and distant metastasis samples from 26 patients. Neoantigens were predicted using pVACseq pipeline. Subtype prediction model was built using random forest algorithm. Portraying the landscape of neoantigens in NPC for the first time, we found that the neoantigen load of NPC was above average compared to that of other cancers in The Cancer Genome Atlas program. While the quantity and quality of neoantigens were similar among primary tumor, regional lymph node metastasis and distant metastasis samples, neoantigen depletion was more severe in metastatic sites than in primary tumors. Upon tracking the clonality change of neoantigens, we found that neoantigen reduction occurred during metastasis. Building a subtype prediction model based on reported data, we observed that subtype I lacked T cells and suffered from severe neoantigen depletion, subtype II highly expressed immune checkpoint molecules and suffered from the least neoantigen depletion, and subtype III was heterogenous. These results indicate that neoantigens are conducive to the guidance of clinical treatment, and personalized therapeutic vaccines for NPC deserve deeper basic and clinical investigations to make them feasible in the future.
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http://dx.doi.org/10.7150/thno.53229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120206PMC
July 2021
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