Publications by authors named "Jun Shi"

692 Publications

Long-term follow-up of 46 cases of primary fallopian tube carcinoma: a single institute study.

Ann Palliat Med 2021 Aug;10(8):9122-9135

Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; Shanghai Key Laboratory of Gynecologic Oncology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China; State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Background: Primary fallopian tube carcinoma (PFTC) is a rare malignancy. In recent years the incidence of PFTC has been rising. This study retrospectively analyzed 46 cases of PFTC to identify prognostic factors that may impact the survival of patients with PFTC and explored the clinical characteristics.

Methods: The clinical data of patients who had undergone surgery and adjuvant chemotherapy in Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University from 1995 to 2015 were retrospectively analyzed. We analyzed clinical data. Cox proportional hazards model was used for univariate and multivariate survival analysis.

Results: The level of CA125 increased in almost all patients with advanced-stage (stage III-IV) carcinoma and about half the patients with early stage (stage I-II) carcinoma. On ultrasound examination, 41 cases had pelvic mass, and five cases had intrauterine space-occupying lesion. Nine patients underwent curettage (19.6%). By the International Federation of Gynecology and Obstetricians (FIGO) staging system, the distribution of patients was 18 at stage I, 16 at stage II, 10 at stage III, and 2 at stage IV. The mainstay of treatment was surgical cytoreduction and platinum-based chemotherapy. Four patients had residual tumors diameter ≤1 cm (R1), 10 had residual tumors diameter >1 cm, and 32 had no macroscopic residual tumor (R0). Forty patients received postoperative intravenous (IV) chemotherapy. The five-year overall survival (OS) was 94.7% in stage I, 80.0% in stage II, 44.4% in stage III, and 0% in stage IV. Univariate and multivariate analysis revealed that residual tumor was independent prognostic variable for OS. Univariate and multivariate analysis revealed that ascites tumor cells and residual tumor were independent prognostic variables for progression free survival (PFS).

Conclusions: Any postmenopausal women with vaginal bleeding, vaginal discharge, or lower abdominal pain should be alert to PFTC. Complete tumor markers and imaging examination should be conducted as soon as possible to improve the early diagnosis rate of the disease. Regardless of whether the operation is a comprehensive staging operation or cytoreductive surgery (CRS), achieving satisfactory R0 can improve OS and PFS. It is important the ascitic fluid is tested for tumor markers in order to predict PFS.
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http://dx.doi.org/10.21037/apm-21-2083DOI Listing
August 2021

PHF6 and JAK3 mutations cooperate to drive T-cell acute lymphoblastic leukemia progression.

Leukemia 2021 Aug 31. Epub 2021 Aug 31.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.

T-cell acute lymphoblastic leukemia (T-ALL) is a malignant hematologic disease caused by gene mutations in T-cell progenitors. As an important epigenetic regulator, PHF6 mutations frequently coexist with JAK3 mutations in T-ALL patients. However, the role(s) of PHF6 mutations in JAK3-driven leukemia remain unclear. Here, the cooperation between JAK3 activation and PHF6 inactivation is examined in leukemia patients and in mice models. We found that the average survival time is shorter in patients with JAK/STAT and PHF6 comutation than that in other patients, suggesting a potential role of PHF6 in leukemia progression. We subsequently found that Phf6 deficiency promotes JAK3-induced T-ALL progression in mice by inhibiting the Bai1-Mdm2-P53 signaling pathway, which is independent of the JAK3/STAT5 signaling pathway. Furthermore, combination therapy with a JAK3 inhibitor (tofacitinib) and a MDM2 inhibitor (idasanutlin) reduces the Phf6 KO and JAK3 leukemia burden in vivo. Taken together, our study suggests that combined treatment with JAK3 and MDM2 inhibitors may potentially increase the drug benefit for T-ALL patients with PHF6 and JAK3 comutation.
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http://dx.doi.org/10.1038/s41375-021-01392-1DOI Listing
August 2021

Internal Forces Analysis of Prestressed Concrete Box Girder Bridge by Using Structural Stressing State Theory.

Materials (Basel) 2021 Aug 19;14(16). Epub 2021 Aug 19.

Key Lab of Structures Dynamic Behavior and Control of the Ministry of Education, Harbin Institute of Technology, Harbin 150090, China.

This paper analyzes the working behavior characteristics of a prestressed concrete transverse large cantilever continuous (PCTLCC) box girder bridge model based on structural stressing state theory and the numerical shape function (NSF) method. At first, the normalized generalized strain energy density (GSED) is established to model the stressing state of the bridge model. Subsequently, the Mann Kendall (M-K) criterion is applied to detect three characteristic loads, respectively, elastic-plastic branch load (200 kN), failure load (300 kN), and progressive failure load (340 kN), and the failure load is found to be the starting load of the damage process of the bridge model, rather than the ultimate load where the structure has been destroyed. Finally, the NSF method is adopted to interpolate the test data, and a detailed analysis for the variation characteristics of the working behavior of the bridge model under loads is performed based on the interpolation results. The characteristic load detection method and experimental data extension method for PCTLCC box girder bridge established in this study can provide valuable references for the design and analysis of such bridges.
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http://dx.doi.org/10.3390/ma14164671DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8400517PMC
August 2021

TPSSI-Net: Fast and Enhanced Two-Path Iterative Network for 3D SAR Sparse Imaging.

IEEE Trans Image Process 2021 ;30:7317-7332

The emerging field of combining compressed sensing (CS) and three-dimensional synthetic aperture radar (3D SAR) imaging has shown significant potential to reduce sampling rate and improve image quality. However, the conventional CS-driven algorithms are always limited by huge computational costs and non-trivial tuning of parameters. In this article, to address this problem, we propose a two-path iterative framework dubbed TPSSI-Net for 3D SAR sparse imaging. By mapping the AMP into a layer-fixed deep neural network, each layer of TPSSI-Net consists of four modules in cascade corresponding to four steps of the AMP optimization. Differently, the Onsager terms in TPSSI-Net are modified to be differentiable and scaled by learnable coefficients. Rather than manually choosing a sparsifying basis, a two-path convolutional neural network (CNN) is developed and embedded in TPSSI-Net for nonlinear sparse representation in the complex-valued domain. All parameters are layer-varied and optimized by end-to-end training based on a channel-wise loss function, bounding both symmetry constraint and measurement fidelity. Finally, extensive SAR imaging experiments, including simulations and real-measured tests, demonstrate the effectiveness and high efficiency of the proposed TPSSI-Net.
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http://dx.doi.org/10.1109/TIP.2021.3104168DOI Listing
January 2021

Study on the Application and Efficacy of Responsibility Nursing in Dialysis Care.

J Healthc Eng 2021 29;2021:2210191. Epub 2021 Jul 29.

Blood Purification Center, Yanghu Branch of Changzhou Second People's Hospital, Changzhou 213000, Jiangsu, China.

Providing high-quality care to patients undergoing hemodialysis (HD) is a priority for nurses. The present study was conducted to explore the experiences of the quality of nursing care among patients, nurses, and caregivers in Yanghu Branch of Changzhou Second People's Hospital, China. A total of 120 hemodialysis patients consecutively admitted to Yanghu Branch of Changzhou Second People's Hospital were enrolled and divided into two groups according to the nursing method they received: control group (routine nursing) and experimental group (responsibility nursing). The two cohorts were observed and compared for alterations of adverse emotions and inflammatory factors, the incidence of complications, pre-and post-nursing sleep quality, life quality, and patients' satisfaction with nursing. After nursing, the Self-Rating Anxiety/Depression Scale (SAS/DS) scores were lower in the experimental group (EG) than in the control group (CG) (both < 0.05). Serum IL-6, hs-CRP, and TNF- were decreased in both groups after nursing and were even lower in EG (both < 0.05). EG had significantly improved sleep quality and life quality than CG, with a higher nursing satisfaction (all < 0.05). This validates that the responsibility nursing for dialysis patients can validly mitigate patients' negative emotions, improve their quality of life, and ensure high-quality dialysis effect, which is feasible for wide popularization and application in clinics.
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http://dx.doi.org/10.1155/2021/2210191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8346309PMC
July 2021

Water compatible supramolecular polymers: recent progress.

Chem Soc Rev 2021 Aug 4. Epub 2021 Aug 4.

Department of Chemistry, The University of Texas at Austin, 105 E. 24th Street A5300, Austin, TX 78712, USA.

Water compatible supramolecular polymers (WCSPs) combine aqueous compatibility with the reversibility and environmental responsiveness of supramolecular polymers. WCSPs have seen application across a number of fields, including stimuli-responsive materials, healable materials, and drug delivery, and are attracting increasing attention from the design, synthesis, and materials perspectives. In this review, we summarize the chemistry of WCSPs from 2016 to mid-2021. For the sake of discussion, we divide WCSPs into five categories based on the core supramolecular approaches at play, namely hydrogen-bonding arrays, electrostatic interactions, large π-conjugated subunits, host-guest interactions, and peptide-based systems, respectively. We discuss both synthesis and polymer structure, as well as the underlying design expectations. The goal of this overview is to deepen our understanding of the strategies that have been exploited to prepare WCSPs, as well as their properties and uses. Thus, a section devoted to potential applications is included in this review.
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http://dx.doi.org/10.1039/d1cs00187fDOI Listing
August 2021

A novel 4-mRNA signature predicts the overall survival in acute myeloid leukemia.

Am J Hematol 2021 Aug 2. Epub 2021 Aug 2.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.

Acute myeloid leukemia (AML) is an aggressive cancer of myeloid cells with high levels of heterogeneity and great variability in prognostic behaviors. Cytogenetic abnormalities and genetic mutations have been widely used in the prognostic stratification of AML to assign patients into different risk categories. Nevertheless, nearly half of AML patients assigned to intermediate risk need more precise prognostic schemes. Here, 336 differentially expressed genes (DEGs) between AML and control samples and 206 genes representing the intratumor heterogeneity of AML were identified. By applying a LASSO Cox regression model, we generated a 4-mRNA prognostic signature comprising KLF9, ENPP4, TUBA4A and CD247. Higher risk scores were significantly associated with shorter overall survival, complex karyotype, and adverse mutations. We then validated the prognostic value of this 4-mRNA signature in two independent cohorts. We also proved that incorporation of the 4-mRNA-based signature in the 2017 European LeukemiaNet (ELN) risk classification could enhance the predictive accuracy of survival in patients with AML. Univariate and multivariate analyses showed that this signature was independent of traditional prognostic factors such as age, WBC count, and unfavorable cytogenetics. Finally, the molecular mechanisms underlying disparate outcomes in high-risk and low-risk AML patients were explored. Therefore, our findings suggest that the 4-mRNA signature refines the risk stratification and prognostic prediction of AML patients.
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http://dx.doi.org/10.1002/ajh.26309DOI Listing
August 2021

Facile Preparation of MoS Nanocomposites for Efficient Potassium-Ion Batteries by Grinding-Promoted Intercalation Exfoliation.

Small 2021 Aug 16;17(34):e2102263. Epub 2021 Jul 16.

Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications (NUPT), Nanjing, 210023, China.

Efficient exfoliations of bulk molybdenum disulfide (MoS ) into few-layered nanosheets in pure phase are highly attractive because of the promising applications of the resulted 2D materials in diversified optoelectronic devices. Here, a new exfoliation method is presented to prepare semiconductive 2D hexagonal phase (2H phase) MoS -cellulose nanocrystal (CNC) nanocomposites using grinding-promoted intercalation exfoliation (GPIE). This method with facile grinding of the bulk MoS and CNC powder followed by conventional liquid-phase exfoliation in water can not only efficiently exfoliate 2H-MoS nanosheets, but also produce the 2H-MoS /CNC 2D nanocomposites simultaneously. Interestingly, the intercalated CNC sandwiched in MoS nanosheets increases the interlayer spacing of 2H-MoS , providing perfect conditions to accommodate the large-sized ions. Therefore, these nanocomposites are good anode materials of potassium-ion batteries (KIBs), showing a high reversible capacity of 203 mAh g at 200 mA g after 300 cycles, a good reversible capacity of 114 mAh g at 500 mA g , and a low decay of 0.02% per cycle over 1500 cycles. With these impressive KIB performances, this efficient GPIE method will open up a new avenue to prepare pure-phase MoS and promising 2D nanocomposites for high-performance device applications.
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http://dx.doi.org/10.1002/smll.202102263DOI Listing
August 2021

Task-Driven Semantic Coding via Reinforcement Learning.

IEEE Trans Image Process 2021 13;30:6307-6320. Epub 2021 Jul 13.

Task-driven semantic video/image coding has drawn considerable attention with the development of intelligent media applications, such as license plate detection, face detection, and medical diagnosis, which focuses on maintaining the semantic information of videos/images. Deep neural network (DNN)-based codecs have been studied for this purpose due to their inherent end-to-end optimization mechanism. However, the traditional hybrid coding framework cannot be optimized in an end-to-end manner, which makes task-driven semantic fidelity metric unable to be automatically integrated into the rate-distortion optimization process. Therefore, it is still attractive and challenging to implement task-driven semantic coding with the traditional hybrid coding framework, which should still be widely used in practical industry for a long time. To solve this challenge, we design semantic maps for different tasks to extract the pixelwise semantic fidelity for videos/images. Instead of directly integrating the semantic fidelity metric into traditional hybrid coding framework, we implement task-driven semantic coding by implementing semantic bit allocation based on reinforcement learning (RL). We formulate the semantic bit allocation problem as a Markov decision process (MDP) and utilize one RL agent to automatically determine the quantization parameters (QPs) for different coding units (CUs) according to the task-driven semantic fidelity metric. Extensive experiments on different tasks, such as classification, detection and segmentation, have demonstrated the superior performance of our approach by achieving an average bitrate saving of 34.39% to 52.62% over the High Efficiency Video Coding (H.265/HEVC) anchor under equivalent task-related semantic fidelity.
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http://dx.doi.org/10.1109/TIP.2021.3091909DOI Listing
July 2021

Diagnosis of Infantile Hip Dysplasia with B-mode Ultrasound via Two-stage Meta-learning Based Deep Exclusivity Regularized Machine.

IEEE J Biomed Health Inform 2021 Jun 30;PP. Epub 2021 Jun 30.

The B-mode ultrasound (BUS) based computer-aided diagnosis (CAD) has shown its effectiveness for developmental dysplasia of the hip (DDH) in infants. In this work, a two-stage meta-learning based deep exclusivity regularized machine (TML-DERM) is proposed for the BUS-based CAD of DDH. TML-DERM integrates deep neural network (DNN) and exclusivity regularized machine into a unified framework to simultaneously improve the feature representation and classification performance. Moreover, the first-stage meta-learning is mainly conducted on the DNN module to alleviate the overfitting issue caused by the significantly increased parameters in DNN, and a random sampling strategy is adopted to self-generate the meta-tasks; while the second-stage meta-learning mainly learns the combination of multiple weak classifiers by a weight vector to improve the classification performance, and also optimizes the unified framework again. The experimental results on a DDH ultrasound dataset show the proposed TML-DERM achieves the superior classification performance with the mean accuracy of 85.89%, sensitivity of 86.54%, and specificity of 85.23%.
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http://dx.doi.org/10.1109/JBHI.2021.3093649DOI Listing
June 2021

Typical Retrograde Type A Dissection After Previous Type B Dissection.

Heart Surg Forum 2021 Jun 26;24(3):E589-E592. Epub 2021 Jun 26.

Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Sichuan, China.

Background: Stanford type B aortic dissection (TBAD) retrograde tears to Stanford type A AD (RTAAD) have been reported only rarely, but are often fatal. Early diagnosis and timely surgery are essential. We present a typical case of RTAAD after the tip of the stent directly damaged the ascending aorta wall.

Case: A 71-year-old woman was admitted to our department for chest pain and back pain for 10 hours. She had undergone coated stent graft implantation surgery a month previously for TBAD. On first impression, we suspected the AD may have progressed or torn retrogradely. RTAAD was confirmed by computed tomography angiography, and we successfully performed open surgery.

Conclusion: RTAAD should be suspected in patients with chest and back pain after endovascular stent repair. Prompt recognition is essential, and early surgical treatment is strongly recommended.
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http://dx.doi.org/10.1532/hsf.3811DOI Listing
June 2021

Comparison on the growth heterogeneity of Vibrio parahaemolyticus coupled with strain sources and genotypes analyses in different oligotrophic conditions.

J Food Prot 2021 Jun 25. Epub 2021 Jun 25.

Shanghai Ocean University.

Vibrio parahaemolyticus is an important food-borne pathogen in aquatic products, which can survive long-term in an oligotrophic environment and maintain pathogenicity. In this study, the growth curves of 38 strains of V.parahaemolyticus (pathogenic and environmental strains) under different oligotrophic conditions (tryptone soy broth (TSB), TSB diluted 2, 4, and 6 times medium) were simulated and their growth heterogeneity was compared. The growth kinetic parameters (maximum specific growth rate ( µ max ) and lag time (LT)) were calculated by the modified Gompertz model. The results showed that oligotrophic conditions affected the growth variability of strains, and the coefficient of variation (CV) of all strains reached the maximum in the 4-fold dilution of TSB. Under different oligotrophic conditions, the LT of the pathogenic strains was shorter than that of the environmental strains, while the µ max of the environmental strains was greater. This indicated that pathogenic strains were more adaptable to the nutrient-deficient environment. The analysis of different genotypes revealed that the strains with genotype tlh + /tdh + /trh - showed a greater growth variability in oligotrophic environments. These results provided theoretical support for the accuracy of the risk assessment of aquatic products.
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http://dx.doi.org/10.4315/JFP-21-089DOI Listing
June 2021

Effect of modified bamboo lignin replacing part of C5 petroleum resin on properties of polyurethane/polysiloxane pressure-sensitive adhesive and its application on the wood substrate.

J Colloid Interface Sci 2021 Nov 8;602:394-405. Epub 2021 Jun 8.

Guangzhou Institute of Chemistry, Chinese Academy of Sciences, Guangzhou 510650, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China; CASH GCC (Nanxiong) Research Institute of New Materials Co., Ltd., Shaoguan 512026, PR China.

This paper reports a fresh and robust strategy to develop polyurethane/polysiloxane pressure-sensitive adhesives (PSAs) with excellent properties by replacing part of C5 petroleum resin with modified lignin. A unique aspect of this work is the use of renewable lignin to obtain modified monomers. The phenolic hydroxyl group of lignin is increased by 21.4% after demethylation, which will help to introduce 6-bromo-1-hexene into the lignin structure through Williamson method. The L lignin and C5 petroleum resin are mixed with polyurethane/polysiloxane prepolymer, and furthermore a series of PSAs are obtained under ultraviolet light. It turns out that L lignin can not only replace part of C5 petroleum resin, but also obtain attractive and controllable features. Especially when the mass ratio of C5 petroleum resin to L lignin is 6:4, compared with pure C5 petroleum resin, the 180° peel strength and the shear strength of PU46 are increased by 24.1% and 91.5% respectively. Additionally, the shear strength on the wood substrate is increased by 320.6%. This study provides an effective method for the preparation of high value-added lignin PSA, and expands the application fields of PSA.
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http://dx.doi.org/10.1016/j.jcis.2021.06.033DOI Listing
November 2021

-Deficient Neutrophils Promote Hematopoietic Stem/Progenitor Cell Proliferation Mesenchymal Stem Cells.

Front Cell Dev Biol 2021 27;9:650599. Epub 2021 May 27.

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, China.

Myeloid cells have been identified as hematopoietic stem cell (HSC)-regulating cells. However, the mechanisms by which myeloid cells regulate the function of HSCs are not fully defined. Our previous study indicated that the HSCs are over-expanded in mice. Here, using and models, we found that -deficient neutrophils remodeled the bone marrow environment and induced expansion of HSCs . Further studies showed that loss of Rheb1 impaired neutrophils' ability to secrete IL-6, led mesenchymal stem cells (MSCs) to produce more SCF, and promote HSC proliferation. We further found that IL-6 suppressed SCF mRNA expression in human MSCs. Interesting, the high level of IL-6 was also related with poor survival of chronic myeloid leukemia (CML) patients, and higher expression of IL-6 in CML cells is associated with the lower expression of SCF in MSCs in patients. Our studies suggested that blocking IL-6 signaling pathway might stimulate MSCs to secrete more SCF, and to support hematopoietic stem/progenitor cells proliferation.
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http://dx.doi.org/10.3389/fcell.2021.650599DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8191467PMC
May 2021

Two Cu(II) and Co(II) Coordination Polymers: Important Values on Colon Cancer Patients by Reducing Insulin Resistance.

J Oleo Sci 2021 ;70(6):827-835

Department of Chemistry, Nanchang University.

In the current study, via utilizing HL (HL = 2,4-di(3',5'-dicarboxylphenyl)benzoic acid), the symmetrical rigid polycarboxylic acid ligand with V-shape geometry, two new coordination polymers containing Cu(II) and Co(II) have been produced, and their chemical formulae respectively are {[Co(L)(HO)]·6HO} (1) and {[HN(Me)][Cu(L)(HO)]·DMF·HO} (2), leading to a variety kinds of coordination patterns of HL and multifunctional skeletons. Their inhibitory activity on the insulin resistance of colon cancer patients was assessed. In addition, the detailed mechanism of the compound was also investigated. Firstly, the detection of enzyme-linked immunosorbent assay was carried out and the Tumor Necrosis Factor-α (TNF-α) level and the Interleukin-1β (IL-1β) level was detected. Then, the glucose concentration was determined with blood glucose meter. Next, the insulin receptor expression levels of β cells were determined with the real time reverse transcription-polymerase chain reaction assay. Ultimately, the cytotoxicity of compounds 1 and 2 was determined with Cell Counting Kit-8 assay.
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http://dx.doi.org/10.5650/jos.ess21013DOI Listing
January 2021

Thermal Safety of Endoscopic Usage in Robot-Assisted Middle Ear Surgery: An Experimental Study.

Front Surg 2021 14;8:659688. Epub 2021 May 14.

Department of Otolaryngology-Head and Neck Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

The widespread application of endoscopic ear surgery (EES), performed through the external auditory canal, has revealed the limitations of the one-handed technique. The RobOtol® (Collin ORL, Bagneux, France) otological robotic system has been introduced to enable two-handed procedures; however, the thermal properties of dedicated endoscopes, which are usually used in neurosurgery, called "neuro-endoscopes," have not yet been clarified for the robotic systems. In this study, we aimed to profile the thermal characteristics of two dedicated neuro-endoscopes, as compared to endoscopes used routinely in manual EES, called "oto-endoscopes," and defined by a smaller diameter and shorter length, and to discuss the safe application of robotic assistance in EES. Two neuro-endoscopes (3.3 mm, 25 cm, 0°/30°) were studied using two routine light sources (LED/xenon), and two routine oto-endoscopes (3 mm, 14 cm, 0°/30°) were initially measured to provide a comprehensive comparison. Light intensities and temperatures were measured at different power settings. The thermal distributions were measured in an open environment and a human temporal bone model of EES. The cooling measures were also studied. Light intensity was correlated with stabilized tip temperatures ( < 0.01, = 0.8719). Under 100% xenon power, the stabilized temperatures at the tips of 0°, 30° neuro-endoscopes, and 0°, 30° oto-endoscopes were 96.1, 60.1, 67.8, and 56.4°C, respectively. With 100% LED power, the temperatures decreased by about 10°C, respectively. For the 0° neuro-endoscope, the illuminated area far away 1cm from the tip was below 37°C when using more than 50% both power, while this distance for 30° neuro-endoscope was 0.5 cm. In the EES temporal bone model, the round window area could reach 59.3°C with the 0° neuro-endoscope under 100% xenon power. Suction resulted in a ~1-2°C temperature drop, while a 10 mL saline rinse gave a baseline temperature which lasted for 2.5 min. Neuro-endoscope causes higher thermal releasing in the surgical cavity of ESS, which should be especially cautious in the robotic system usage. Applying submaximal light intensity, a LED source and intermittent rinsing should be considered for the safer robot-assisted EES using a neuro-endoscope that allows a two-handed surgical procedure.
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http://dx.doi.org/10.3389/fsurg.2021.659688DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8160440PMC
May 2021

Simultaneous removal of nitrate and diethyl phthalate using a novel sponge-based biocarrier combined modified walnut shell biochar with FeO in the immobilized bioreactor.

J Hazard Mater 2021 07 4;414:125578. Epub 2021 Mar 4.

School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; Shaanxi Key Laboratory of Environmental Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.

A novel biological carrier combining sponge and modified walnut shell biochar with FeO ([email protected]) was fabricated to achieve simultaneous removal of nitrate and diethyl phthalate (DEP). The optimal reaction conditions of the immobilized bioreactor were: carbon to nitrogen (C/N) ratio of 1.5, Fe concentration of 20 mg L, and hydraulic retention time (HRT) of 8 h. Under the optimal conditions and DEP concentration of 800 μg L, the highest removal efficiency of DEP and nitrate in the immobilized bioreactor with the novel biological carrier were 67.87% and 83.97% (68.43 μg L h and 1.71 mg L h), respectively. Scanning electron microscopy (SEM) showed that the novel biological carrier in this study carried more bio-sediments which is closely related to the denitrification efficiency. The gas chromatography (GC) data showed that the nitrogen production of the immobilized bioreactor (99.85%) was higher than that of another experimental group (97.84%). Fluorescence excitation-emission matrix (EEM) and Fourier transform infrared spectrometer (FTIR) indicated the immobilized bioreactor emerged more extracellular polymeric substances (EPS) which was related to favourable biological stability under the DEP environment. Moreover, according to high-throughput sequencing data, the Zoogloea sp. L2 responsible for iron-reduction and denitrification was the main strain in this immobilized bioreactor.
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http://dx.doi.org/10.1016/j.jhazmat.2021.125578DOI Listing
July 2021

Downregulation of USP18 reduces tumor-infiltrating activated dendritic cells in extranodal diffuse large B cell lymphoma patients.

Aging (Albany NY) 2021 05 17;13(10):14131-14158. Epub 2021 May 17.

Department of Hematology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Extranodal diffuse large B cell lymphoma (EN DLBCL) often leads to poor outcomes, while the underlying mechanism remains unclear. As immune imbalance plays an important role in lymphoma pathogenesis, we hypothesized that immune genes might be involved in the development of EN DLBCL. Ninety-three differentially expressed immune genes (DEIGs) were identified from 1168 differentially expressed genes (DEGs) between tumor tissues of lymph node DLBCL (LN DLBCL) and EN DLBCL patients in TCGA database. Nine prognostic immune genes were further identified from DEIGs by univariate Cox regression analysis. A multivariate predictive model was established based on these prognostic immune genes. Patients were divided into high- and low-risk groups according to the median model-based risk score. Kaplan-Meier survival curves showed that patients in the high-risk group had a shorter survival time than those in the low-risk group (P < 0.001). Ubiquitin-specific peptidase 18 (USP18) was further recognized as the key immune gene in EN DLBCL on the basis of coexpression of differentially expressed transcription factors (DETFs) and prognostic immune genes. USP18 exhibited low expression in EN DLBCL, which was regulated by LIM homeobox 2 (LHX2) (R = 0.497, P < 0.001, positive). The potential pathway downstream of USP18 was the MAPK pathway, identified by gene set variation analysis (GSVA), gene set enrichment analysis (GSEA) and Pearson correlation analysis (R = 0.294, P < 0.05, positive). The "ssGSEA" algorithm and Pearson correlation analysis identified that activated dendritic cells (aDCs) were the cell type mostly associated with USP18 (R = 0.694, P < 0.001, positive), indicating that USP18 participated in DC-modulating immune responses. The correlations among key biomarkers were supported by multiomics database validation. Indeed, the USP18 protein was confirmed to be expressed at lower levels in tumor tissues in patients with EN DLBCL than in those with LN DLBCL by immunohistochemistry. In short, our study illustrated that the downregulation of USP18 was associated with reduced aDC number in the tumor tissues of EN DLBCL patients, indicating that targeting USP18 might serve as a promising therapy.
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http://dx.doi.org/10.18632/aging.203030DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202869PMC
May 2021

HBCD, TBECH, and BTBPE exhibit cytotoxic effects in human vascular endothelial cells by regulating mitochondria function and ROS production.

Environ Toxicol 2021 Aug 11;36(8):1674-1682. Epub 2021 May 11.

Division of Cardiology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Brominated flame retardants (BFRs), such as, 1,2,5,6-tetrabromocyclooctane (HBCD), 1,2-dibromo-4-(1,2-dibromopropyl)cyclohexane (TBECH), and 1 1,2-bis-(2,4,6-tribromophenoxy)ethane (BTBPE), have garnered increasing attention due to their potent biological effects. In the present study, the toxicity of HBCD, TBECH, and BTBPE in human vascular endothelial cells (ECs) was explored. The data showed that HBCD, TBECH, and BTBPE induced cytotoxicity, namely dose-dependent cell viability reduction, cell membrane permeability and apoptosis increase, migration, and lumen formation inhibition. Moreover, HBCD was found to be more toxic than BTBPE or TBECH. Exposure to HBCD, TBECH, and BTBPE led to the production of reactive oxygen species, mitochondrial superoxide generation, and mitochondrial membrane potential collapse, implying that reactive stress caused the cytotoxicity. The ATP content, glutathione content, superoxide dismutase, and MDA activities were reduced, indicating that mitochondrial dysfunction may be the key mechanisms responsible for apoptosis. The present study suggested that mitochondria are a new target of BFRs in ECs and further deepened our understanding of the developmental toxicity of BFRs.
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http://dx.doi.org/10.1002/tox.23163DOI Listing
August 2021

Good treatment-free survival of monoclonal gammopathy of undetermined significance associated pure red cell aplasia after bortezomib plus dexamethasone.

Blood Cells Mol Dis 2021 07 27;89:102573. Epub 2021 Apr 27.

Regenerative Medicine Clinic, State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China. Electronic address:

Pure red cell aplasia (PRCA) is a rare syndrome characterized by severe anemia and absence of erythroid precursors. PRCA associated to monoclonal gammopathy of undetermined significance (MGUS) is a scarce condition with less than five cases reported so far. There is no agreement on the treatment of MGUS associated PRCA and treatment- free survival (TFS) is an unmet clinical need. In this report, for the first time, we demonstrated two patients with MGUS associated PRCA obtained rapid remission and maintained TFS after accepting intensive short-term bortezomib plus dexamethasone. The first case was refractory to cyclosporine and prednisone, but achieved complete remission after ten doses of bortezomib. Moreover, he has kept TFS for 12 months. The other case initiated bortezomib plus dexamethasone as soon as making a definite diagnosis. She obtained complete remission after twelve doses of bortezomib and she has maintained a normal level of haemoglobin for 8 months.
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http://dx.doi.org/10.1016/j.bcmd.2021.102573DOI Listing
July 2021

Coronary artery bypass grafting in a patient with situs inversus totalis and Hodgkin lymphoma: Three-year follow-up report and systematic literature review.

Asian J Surg 2021 Jun 2;44(6):885-886. Epub 2021 May 2.

Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China. Electronic address:

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http://dx.doi.org/10.1016/j.asjsur.2021.03.043DOI Listing
June 2021

Chitosan/hyaluronic acid based hollow microcapsules equipped with MXene/gold nanorods for synergistically enhanced near infrared responsive drug delivery.

Int J Biol Macromol 2021 Jul 1;183:870-879. Epub 2021 May 1.

School of Materials Science and Engineering, Zhengzhou University, Zhengzhou 450001, China; Henan Key Laboratory of Advanced Nylon Materials and Application, Zhengzhou University, Zhengzhou 450001, China. Electronic address:

TiC MXenes, a novel two-dimensional material, have attracted lots of attention in biomedical filed for its large surface area and excellent near-infrared (NIR) responsiveness. In this paper, a pH/near-infrared (NIR) multi-responsive drug delivery platform consisted of hollow hydroxyapatite (HAP), chitosan (CS)/hyaluronic acid (HA) multilayers, gold nanorods (AuNRs) and MXene had been fabricated via a layer-by-layer (LbL) approach. Chitosan/hyaluronic acid multilayers were deposited on the surface of hollow HAP to retard the burst release of DOX in the initial delivery stage. MXenes and AuNRs equipped on the surface of hybrid matrix greatly enhanced the photothermal conversion efficiency of the microcapsules. Due to the collapse of electrostatic force among chitosan/hyaluronic acid multilayers and the dissolution of HAP under acidic condition, as well as the synergistically enhanced photothermal effect between MXene and AuNRs, HAP/CS/HA/MXene/AuNRs microcapsules exhibited outstanding pH-/NIR-responsive drug delivery properties. The present paper provides an attractive method to prepare chitosan/hyaluronic acid based pH/NIR multi-responsive hybrid microcapsules with excellent photothermal conversion efficiency and biocompatibility, which has great potential in the field of remotely controlled drug delivery.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.04.164DOI Listing
July 2021

LncRNA lnc-ISG20 promotes renal fibrosis in diabetic nephropathy by inducing AKT phosphorylation through miR-486-5p/NFAT5.

J Cell Mol Med 2021 06 3;25(11):4922-4937. Epub 2021 May 3.

Department of Nephrology, Huaihe Hospital of Henan University, Kaifeng, China.

Long non-coding RNA (lncRNA) lnc-ISG20 has been found aberrantly up-regulated in the glomerular in the patients with diabetic nephropathy (DN). We aimed to elucidate the function and regulatory mechanism of lncRNA lnc-ISG20 on DN-induced renal fibrosis. Expression patterns of lnc-ISG20 in kidney tissues of DN patients were determined by RT-qPCR. Mouse models of DN were constructed, while MCs were cultured under normal glucose (NG)/high glucose (HG) conditions. The expression patterns of fibrosis marker proteins collagen IV, fibronectin and TGF-β1 were measured with Western blot assay. In addition, the relationship among lnc-ISG20, miR-486-5p, NFAT5 and AKT were analysed using dual-luciferase reporter assay and RNA immunoprecipitation. The effect of lnc-ISG20 and miR-486/NFAT5/p-AKT axis on DN-associated renal fibrosis was also verified by means of rescue experiments. The expression levels of lnc-ISG20 were increased in DN patients, DN mouse kidney tissues and HG-treated MCs. Lnc-ISG20 silencing alleviated HG-induced fibrosis in MCs and delayed renal fibrosis in DN mice. Mechanistically, miR-486-5p was found to be a downstream miRNA of lnc-ISG20, while miR-486-5p inhibited the expression of NFAT5 by binding to its 3'UTR. NFAT5 overexpression aggravated HG-induced fibrosis by stimulating AKT phosphorylation. However, NFAT5 silencing reversed the promotion of in vitro and in vivo fibrosis caused by lnc-ISG20 overexpression. Our collective findings indicate that lnc-ISG20 promotes the renal fibrosis process in DN by activating AKT through the miR-486-5p/NFAT5 axis. High-expression levels of lnc-ISG20 may be a useful indicator for DN.
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http://dx.doi.org/10.1111/jcmm.16280DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178263PMC
June 2021

Tetramethylpyrazine Ameliorates Peritoneal Angiogenesis by Regulating VEGF/Hippo/YAP Signaling.

Front Pharmacol 2021 13;12:649581. Epub 2021 Apr 13.

Department of Nephrology, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, China.

Angiogenesis of human peritoneal vascular endothelial cells (HPVECs), linked to vascular endothelial growth factor (VEGF)/VEGF receptor 2 (VEGFR2) signaling, is a complication of peritoneal fibrosis (PF). Hippo/YAP signaling interacts with VEGF/VEGFR2 signaling, but the effect on peritoneal angiogenesis and PF has not been studied. We tested VEGF/Hippo/YAP inhibition by tetramethylpyrazine (TMP) in PF mice and HPVECs. This treatment ameliorated peritoneal dialysis (PD)-induced angiogenesis and PF. In mice, PF was associated with upregulation of VEGF, and TMP ameliorated submesothelial fibrosis, perivascular bleeding, and Collagen I abundance. In HPVECs, angiogenesis occurred due to human peritoneal mesothelial cells (HPMCs)-conditioned medium, and TMP alleviated HPVECs migration, tube formation, and YAP nuclear translocation. YAP knockdown PF mouse and HPVEC models were established to further confirm our finding. YAP deletion attenuated the PD-induced or VEGF-induced increase in angiogenesis and PF. The amount of CYR61 and CTGF was significantly less in the YAP knockdown group. To study the possibility that TMP could benefit angiogenesis, we measured the HPVECs migration and tube formation and found that both were sharply increased in YAP overexpression; TMP treatment partly abolished these increases. As well, the amount of VEGFR localized in the trans-Golgi network was lower by double immunofluorescence; VEGFR and its downstream signaling pathways including p-ERK, p-P38, and p-Akt were more in HPVECs with YAP overexpression. Overall, TMP treatment ameliorated angiogenesis, PF, and peritoneum injury. These changes were accompanied by inhibition of VEGF/Hippo/YAP.
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http://dx.doi.org/10.3389/fphar.2021.649581DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076865PMC
April 2021

[Application of multiple empirical kernel mapping ensemble classifier based on self-paced learning in ultrasound-based computer-aided diagnosis for breast cancer].

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2021 Feb;38(1):30-38

Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, P.R. China.

Both feature representation and classifier performance are important factors that determine the performance of computer-aided diagnosis (CAD) systems. In order to improve the performance of ultrasound-based CAD for breast cancers, a novel multiple empirical kernel mapping (MEKM) exclusivity regularized machine (ERM) ensemble classifier algorithm based on self-paced learning (SPL) is proposed, which simultaneously promotes the performance of both feature representation and the classifier. The proposed algorithm first generates multiple groups of features by MEKM to enhance the ability of feature representation, which also work as the kernel transform in multiple support vector machines embedded in ERM. The SPL strategy is then adopted to adaptively select samples from easy to hard so as to gradually train the ERM classifier model with improved performance. This algorithm is verified on a B-mode ultrasound dataset and an elastography ultrasound dataset, respectively. The results show that the classification accuracy, sensitivity and specificity on B-mode ultrasound are (86.36±6.45)%, (88.15±7.12)%, and (84.52±9.38)%, respectively, and the classification accuracy, sensitivity and specificity on elastography ultrasound are (85.97±3.75)%, (85.93±6.09)%, and (86.03±5.88)%, respectively. It indicates that the proposed algorithm can effectively improve the performance of ultrasound-based CAD for breast cancers with the potential for application.
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http://dx.doi.org/10.7507/1001-5515.202002004DOI Listing
February 2021

miR-29c&b2 encourage extramedullary infiltration resulting in the poor prognosis of acute myeloid leukemia.

Oncogene 2021 May 22;40(19):3434-3448. Epub 2021 Apr 22.

Department of Hematology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

Extramedullary infiltration (EMI), as a concomitant symptom of acute myeloid leukemia (AML), is associated with low complete remission and poor prognosis in AML. However, the mechanism of EMI remains indistinct. Clinical trials showed that increased miR-29s were associated with a poor overall survival in AML [14]. Nevertheless, they were proved to work as tumor suppressor genes by encouraging apoptosis and inhibiting proliferation in vitro. These contradictory results led us to the hypothesis that miR-29s may play a notable role in the prognosis of AML rather than leukemogenesis. Thus, we explored the specimens of AML patients and addressed this issue into miR-29c&b2 knockout mice. As a result, a poor overall survival and invasive blast cells were observed in high miR-29c&b2-expression patients, and the wildtype mice presented a shorter survival with heavier leukemia infiltration in extramedullary organs. Subsequently, we found that the miR-29c&b2 inside leukemia cells promoted EMI, but not the one in the microenvironment. The analysis of signal pathway revealed that miR-29c&b2 could target HMG-box transcription factor 1 (Hbp1) directly, then reduced Hbp1 bound to the promoter of non-muscle myosin IIB (Myh10) as a transcript inhibitor. Thus, increased Myh10 encouraged the migration of leukemia cells. Accordingly, AML patients with EMI were confirmed to have high miR-29c&b2 and MYH10 with low HBP1. Therefore, we identify that miR-29c&b2 contribute to the poor prognosis of AML patients by promoting EMI, and related genes analyses are prospectively feasible in assessment of AML outcome.
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http://dx.doi.org/10.1038/s41388-021-01775-9DOI Listing
May 2021

SNHG1/miR-186/FUT8 regulates cell migration and invasion in oral squamous cell carcinoma.

Oral Dis 2021 Apr 19. Epub 2021 Apr 19.

Department of Stomatology, XD Group Hospital, Xi'an, China.

Recently, lncRNAs are associated with the progression and development of various cancers. We aimed to explore the effects of lncRNA SNHG1 on the proliferation, apoptosis, migration, and invasion of oral squamous cell carcinoma (OSCC) cells. Quantitative real-time PCR (RT-qPCR) was used for measurement of SNHG1 in OSCC cells. Cell proliferation, apoptosis, migration, and invasion were detected by CCK-8 assay, flow cytometry, Cell Death Detection ELISA PLUS kit, and transwell assays. Dual-luciferase reporter assay and RNA-binding protein immunoprecipitation (RIP) assay were used to clarify the relationship between SNHG1 and miR-186. SNHG1 was overexpressed in OSCC cells. SNHG1 silencing prevented cell proliferation and increased the incidence of apoptosis, DNA fragments, cleaved-caspase 3, and Bax protein levels. Cell migration and invasion were reduced after SNHG1 deletion, and MMP2 and MMP9 protein levels were decreased. SNHG1 overexpression promoted cell survival, migration, and invasion, reduced DNA fragments formation. Mechanistically, we demonstrated that SNHG1 could directly bind to miR-186 and positively regulated α1, 6-fucosyltransferase (FUT8) level. Functional investigation showed that miR-186 depletion reversed the roles of SNHG1 silencing in cell proliferation, apoptosis, and migration. Taken together, our findings illuminated that SNHG1 regulated cell proliferation, migration, and invasion by sponging miR-186 to depress FUT8 expression.
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http://dx.doi.org/10.1111/odi.13878DOI Listing
April 2021

Multi-Source Transfer Learning via Multi-Kernel Support Vector Machine Plus for B-Mode Ultrasound-Based Computer-Aided Diagnosis of Liver Cancers.

IEEE J Biomed Health Inform 2021 Apr 16;PP. Epub 2021 Apr 16.

B-mode ultrasound (BUS) imaging is a routine tool for diagnosis of liver cancers, while contrast-enhanced ultrasound (CEUS) provides additional information to BUS on the local tissue vascularization and perfusion to promote diagnostic accuracy. In this work, we propose to improve the BUS-based computer aided diagnosis for liver cancers by transferring knowledge from the multi-view CEUS images, including the arterial phase, portal venous phase, and delayed phase, respectively. To make full use of the shared labels of paired of BUS and CEUS images to guide knowledge transfer, support vector machine plus (SVM+), a specifically designed transfer learning (TL) classifier for paired data with shared labels, is adopted for this supervised TL. A nonparallel hyperplane based SVM+ (NHSVM+) is first proposed to improve the TL performance by transferring the per-class knowledge from source domain to the corresponding target domain. Moreover, to handle the issue of multi-source TL, a multi-kernel learning based NHSVM+ (MKL-NHSVM+) algorithm is further developed to effectively transfer multi-source knowledge from multi-view CEUS images. The experimental results indicate that the proposed MKL-NHSVM+ outperforms all the compared algorithms for diagnosis of liver cancers, whose mean classification accuracy, sensitivity, and specificity are 88.183.16%, 86.984.77%, and 89.423.77%, respectively.
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http://dx.doi.org/10.1109/JBHI.2021.3073812DOI Listing
April 2021

[Optimization of Mouse Model of Aplastic Anemia Induced by Pan T Lymphocytes Combined with Irradiation].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2021 Apr;29(2):557-566

State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020,

AbstractObjective: To establish an acquired aplastic anemia animal model for investigating the function of T lymphocyte and the pathogenesis and treatment of aplastic anemia(AA).

Methods: To establish the acquired aplastic anemia mouse model through the X-ray irradiation in combination with lymphocytes injection. AA Group: the purified Pan T lymphocytes from the spleen of C57BL/6J mice were enriched and injected to the mice through tail vein(5×10), the CB6F1 mice were exposed to 3,4 and 5 Gy X-ray irradiation; TBI Group: the CB6F1 mice were exposed to 3,4 and 5 Gy X-ray irradiation, and were injected with the same volume of PBS buffer; Control group: the CB6F1 mice were only injected with the same volume of PBS buffer. The peripheral blood routine was examined and the number of nucleated cells in bone marrow were calculated;the hematopoiesis changes in bone marrow was examined;flow cytometry was used to examine the distribution of T lymphocytes in bone marrow, and it also used to examine the apoptosis of bone marrow cells and the differentiation of spleen T lymphocytes.

Results: Compared with 4, 5 Gy irradiated mice in AA groups, the survival time of 3 Gy irradiated AA groups was significantly prolonged. 3, 4 and 5 Gy X-ray irradiation combined with Pan T lymphocyte injection could successfully induced severe reduction of red blood cells, blood neutrophils, and platelets, severe reduction of bone marrow nucleated cells, severe bone marrow hematopoietic failure, and the significant expansion of T lymphocytes ratio in the bone marrow. CD4 and CD8 T cells were both increased, but mainly on CD8 T cells, and could promote the differentiation of T cells from naïve T cells to effector memory T cells.

Conclusion: 3, 4 and 5 Gy X-ray irradiation combined with 5×10 pan-T cell injection could successfully induce acquired aplastic anemia through T lymphocyte hyperfunction. Compared with 4, 5 Gy irradiated AA group, the 3 Gy irradiated AA group shows significantly longer survival time, and the peripheral blood routine profile closely resembles the clinical manifestations of AA patients.
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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2021.02.040DOI Listing
April 2021

Co-Delivery of Paclitaxel and siRNA with pH-Responsive Polymeric Micelles for Synergistic Cancer Therapy.

J Biomed Nanotechnol 2021 Feb;17(2):322-329

Henan Eye Hospital, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, P. R. China.

Due to the complex physiological characteristics of tumors, chemotherapy or gene therapy alone cannot completely kill tumor cells. Therefore, combining chemotherapy with gene therapy for combination therapy is the key to solving this problem. However, there are still significant challenges in how to simultaneously deliver and rapidly release the drugs and siRNA into cancer cells. In this work, a triblock copolymer was synthesized to co-deliver siRNA and paclitaxel to tumor cells. This system has an acid-sensitive subsurface layer, which can not only load siRNA to prevent premature drug release but also has good controlled release performance. experiments showed that polymeric vectors can efficiently deliver siRNA and paclitaxel simultaneously into tumor cells for rapid release within the tumor cells. This study reveals that this novel polymeric micelle is a suitable vector for the codelivery of chemotherapeutic drugs and siRNA to cancer cells, representing an important advance in nanotechnology, nanomedicine, drug delivery, and cancer therapy.
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http://dx.doi.org/10.1166/jbn.2021.3039DOI Listing
February 2021
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