Publications by authors named "Jun Luo"

1,361 Publications

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Regulates the Transcription of AR Target Genes by Antagonizing NONO.

Life (Basel) 2021 Nov 9;11(11). Epub 2021 Nov 9.

Department of Urology, Shanghai Fourth People's Hospital Affiliated to Tongji University School of Medicine, Shanghai 200434, China.

The androgen receptor (AR) and its related signaling pathways play an important role in the development of prostate cancer (PCa). Long non-coding RNAs (lncRNAs) are involved in the regulation of tumorigenesis and development, but their specific mechanism of action remains unclear. This study examines the function and mechanisms of action of lncRNA in the development of PCa. It shows that dihydrotestosterone (DHT) results in the AR-dependent suppression of expression in the LNCaP androgen-sensitive human prostate adenocarcinoma cell line. In addition, overexpression of inhibits the proliferation and migration of PCa cells, and suppresses the expression of AR target genes. This research also demonstrates that the protein NONO interacts with AR and functions as an AR co-activator, promoting AR transcriptional activity. Furthermore, using RNA immunoprecipitation (RIP)-PCR experiments, the study demonstrates that both NONO and AR can bind . Moreover, cell phenotypic experiments reveal that NONO can promote cellular proliferation and migration, and that can partially antagonize the pro-oncogenic functions of NONO in PCa cells. In summary, the results indicate that can bind NONO, suppressing its ability to promote AR-dependent transcriptional activity. Furthermore, DHT-dependent suppression of expression can enhance NONO's promotion effect on AR.
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http://dx.doi.org/10.3390/life11111208DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625561PMC
November 2021

Effectiveness and Safety of DOACs vs. VKAs in AF Patients With Cancer: Evidence From Randomized Clinical Trials and Observational Studies.

Front Cardiovasc Med 2021 5;8:766377. Epub 2021 Nov 5.

Department of Cardiology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.

The use of direct oral anticoagulants (DOACs) is recommended as the preferred treatment drug in patients with nonvalvular atrial fibrillation (AF). However, the effectiveness and safety of DOACs compared with vitamin K antagonists (VKAs) in patients with cancer and AF are still controversial. Therefore, we performed a meta-analysis regarding the effectiveness and safety of DOACs vs. VKAs in AF patients with cancer. A search of the Pubmed and EMBASE databases until August 2021 was performed. Adjusted risk ratios (RRs) and 95% confidence intervals (CIs) were pooled using a random-effects model with an inverse variance method. Thirteen studies were deemed to meet the criteria. For the effectiveness outcomes, the use of DOACs compared with VKAs use was significantly associated with decreased risks of stroke or systemic embolism (RR = 0.66, 95% CI: 0.54-0.80) and venous thromboembolism (RR = 0.40, 95% CI: 0.26-0.61), but not ischemic stroke (RR = 0.79, 95% CI: 0.56-1.11), myocardial infarction (RR = 0.78, 95% CI: 0.56-1.11), cardiovascular death (RR = 0.76, 95% CI: 0.53-1.09), and all-cause death (RR = 0.82, 95% CI: 0.43-1.56). For the safety outcomes, compared with VKAs use, the use of DOACs was associated with reduced risks of intracranial bleeding (RR = 0.60, 95% CI: 0.50-0.71) and gastrointestinal bleeding (RR = 0.87, 95% CI: 0.80-0.95). There were no significant differences in major bleeding (RR = 0.87, 95% CI: 0.74-1.04), major or nonmajor clinically relevant bleeding (RR = 0.87, 95% CI: 0.74-1.01), and any bleeding (RR = 0.88, 95% CI: 0.76-1.03). Compared with VKAs, DOACs appeared to have significant reductions in stroke or systemic embolism, venous thromboembolism, intracranial bleeding, and gastrointestinal bleeding, but comparable risks of ischemic stroke, myocardial infarction, cardiovascular death, all-cause death, major bleeding, major or nonmajor clinically relevant bleeding, and any bleeding in patients with AF and cancer.
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http://dx.doi.org/10.3389/fcvm.2021.766377DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8602680PMC
November 2021

The Electrical and Thermal Transport Properties of La-Doped SrTiO with ScO Composite.

Materials (Basel) 2021 Oct 21;14(21). Epub 2021 Oct 21.

School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, China.

Donor-doped strontium titanate (SrTiO) is one of the most promising n-type oxide thermoelectric materials. Routine doping of La at Sr site can change the charge scattering mechanism, and meanwhile can significantly increase the power factor in the temperature range of 423-773 K. In addition, the introduction of Sc partially substitutes Sr, thus further increasing the electron concentration and optimizing the electrical transport properties. Moreover, the excess Sc in the form of ScO composite suppresses multifrequency phonon transport, leading to low thermal conductivity of = 3.78 W·m·K at 773 K for sample SrLaScTiO with the highest doping content. Thus, the thermoelectric performance of SrTiO can be significantly enhanced by synergistic optimization of electrical transport and thermal transport properties via cation doping and composite engineering.
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http://dx.doi.org/10.3390/ma14216279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585205PMC
October 2021

Mercury ion-engineering Au plasmonics on MoS layers for absorption-shifted optical sensors.

Anal Methods 2021 Nov 25;13(45):5436-5440. Epub 2021 Nov 25.

Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.

Semiconducting MoS layers offer the electrons, reducing conjugated Au(I) to Au atoms, and sebsequently serve as desirable substrates for supporting the interfacial growths of gold nanostructures. Au-covering MoS heterostructures perform morphology-varied optical characteristics, and the surface engineering of MoS involved by Hg ions results in the differential growths of nanostructures and morphological diversities. Naked-eye colorimetric responses to mercury ions, with a low limit of detection of 1.27 nM, are achieved based on the grown heterostructures.
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http://dx.doi.org/10.1039/d1ay01637gDOI Listing
November 2021

Integrated analysis of hub gene expression in multiple myeloma.

J BUON 2021 Sep-Oct;26(5):2040-2052

Department of Hematology, the First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Purpose: To explore the expression and clinical significance of factors associated with multiple myeloma (MM) and identify new diagnostic markers.

Methods: Two gene expression array data sets (GSE6477 and GSE5900) were downloaded and differentially expressed genes (DEGs) in bone marrow from patients with MM and healthy donors analyzed. Kyoto Encyclopedia of Genes and Genomes pathway enrichment and Gene Ontology annotation of DEGs was conducted and a protein-protein interaction network generated. Plasma and bone marrow samples from patients with MM were analyzed for cytokine expression by ELISA and correlations between cytokine levels and clinical indicators evaluated.

Results: Of 908 DEGs, 416 were up-regulated and 492 down-regulated. Further, 161 proteins pairs and 21 nodes were detected, and eight hub genes (CXCL2, CXCL8, CXCL12, ELANE, LCN2, CX3CL1, CCL13, and CCL27) screened out. Expression levels of CXCL8, CXCL2, CXCL12, LCN2, and CCL13 were low in CD138+ plasma cells, and expression levels of the eight cytokines differed significantly in peripheral blood plasma from patients with MM and healthy controls. ROC curve analysis determined optimal diagnostic thresholds determined for: CCL27 (189 ng/mL), CXCL2 (313 ng/L), CX3CL1 (132 ng/L), CCL13 (235 pg/mL), CXCL8 (884 ng/L), ELANE (50 µg/L), LCN2 (8 µg/L), and CXCL12 (2525 pg/mL).

Conclusions: CX3CL1, CCL13, CXCL8, and CXCL12 levels were positively correlated with those of hemoglobin and β2 microglobulin (β2-MG); CCL27 and CXCL2 with β2-MG; and CCL13 and ELANE with white blood cell count and age, respectively. CCL27, CXCL2, and β2-MG levels were associated with MM incidence.
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November 2021

Development and application of a high-sensitivity immunochromatographic test strip for detecting classical swine fever virus antibodies.

Transbound Emerg Dis 2021 Nov 1. Epub 2021 Nov 1.

Key Laboratory of Animal Immunology, Ministry of Agriculture and Rural Affairs & Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, People's Republic of China.

Classical swine fever (CSF) is caused by classical swine fever virus (CSFV) and has led to huge economic losses in the pig industry worldwide. Although vaccination and other control measures have been carried out, it is essential to establish a rapid and valid method for CSF vaccination monitoring and clinical diagnosis. The CSFV E2 protein has been widely used as a major antigen for antibody detection. It is important to improve the affinity between the E2 protein and CSFV antibodies to improve the performance of the detection method. In this study, a recombinant E2 extracellular protein (amino acids 1-331) with a native homodimer conformation and high affinity for the anti-CSFV-E2 monoclonal antibody WH303 was expressed using a Bac-to-Bac baculovirus expression system. A novel immunochromatographic test strip based on the recombinant CSFV E2 protein was developed for CSFV antibody detection. The sensitivity of this strip for detecting CSFV standard-positive serum was 1:102400, 4 times higher than that of the previously developed CnC2 test strip. No cross-reactivity with antibodies of other swine viruses was observed. Detection of clinical swine serum samples (n = 813) demonstrated that the agreements of this E2 test strip with three commercial ELISA kits were 97.17% (790/813), 95.94% (780/813), and 93.73% (762/813), respectively. Our data indicate that a novel E2 test strip with enhanced sensitivity has been developed and can be applied for clinical sample detection, providing a new, powerful and simple approach for CSFV antibody monitoring. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1111/tbed.14367DOI Listing
November 2021

Silk fibroin hydrogels induced and reinforced by acidic calcium phosphate - A simple way of producing bioactive and drug-loadable composites for biomedical applications.

Int J Biol Macromol 2021 Dec 26;193(Pt A):433-440. Epub 2021 Oct 26.

Division of Applied Materials Science, Department of Materials Science and Engineering, Uppsala University, Uppsala, Box 534, 751 21, Sweden; Division of Biomedical Engineering, Department of Materials Science and Engineering, Uppsala University, Uppsala, Box 534, 751 21, Sweden. Electronic address:

Silk fibroin (SF) hydrogels have attracted extensive interest in biomedical applications due to their biocompatibility and wide availability. However, their generally poor mechanical properties limit their utility. Here, injectable, ready-to-use SF-based composites, simultaneously induced and reinforced by acidic calcium phosphates, were prepared via a dual-paste system requiring no complex chemical/physical treatment. The composite was formed by mixing a monocalcium phosphate monohydrate paste with a β-tricalcium phosphate/SF paste. The conformational transition of SF in an acidic environment forms continuous networks, and the acidic calcium phosphate, brushite and monetite, formed simultaneously in the networks during mixing. The composites displayed a partly elastomeric compression behavior, with mechanical properties increasing with an increasing calcium phosphate and β-sheet content at the lower calcium phosphate contents evaluated (22.2-36.4 wt%). While the stiffness was still relatively low, the materials presented a high elasticity and ductility, and no failure at stresses in the range of failure stresses of trabecular bone. Furthermore, the calcium phosphate confers bioactivity to the material, and the composites with a promising in vitro cell response also showed potential as drug vehicles, using vancomycin as a model drug. These dual-paste systems exhibit potential utility in biomedical applications, such as bone void fillers and drug vehicles.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.10.160DOI Listing
December 2021

Construction and Integrated Analysis of Competitive Endogenous Long Non-Coding RNA Network in Thoracic Aortic Dissection.

Int J Gen Med 2021 16;14:6863-6873. Epub 2021 Oct 16.

Department of Cardiothoracic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, People's Republic of China.

Background: Long non-coding RNAs (lncRNAs) can act as a competitive endogenous RNA (ceRNA) to regulate gene expression by sequestering the microRNA (miRNA). However, the lncRNA-miRNA-mRNA ceRNA network in thoracic aortic dissection (TAD) has been rarely documented.

Methods: Three Gene Expression Omnibus (GEO) datasets were used to detect differentially expressed mRNAs, miRNAs, and lncRNAs in TAD. Gene ontology and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were conducted for the differentially expressed mRNAs. A protein-protein interaction network for differentially expressed mRNAs was also constructed, and hub genes were identified. We established a ceRNA network of TAD based on the differentially expressed miRNAs, mRNAs and lncRNAs, and verified our results using an independent dataset and quantitative real-time PCR (qRT-PCR).

Results: In TAD, 267 lncRNAs, 81 miRNAs, and 346 mRNAs were identified as differentially expressed. The established ceRNA network consisted of seven lncRNA nodes, three mRNA nodes, and three miRNA nodes, and the expression of miRNAs in TAD was opposite to that of lncRNAs and mRNAs. Subsequently, an independent GEO dataset and qRT-PCR were used to validate the expression of three mRNAs. In addition, the expression differences in SLC7A5, associated miRNA and lncRNA were verified. According to gene set enrichment analysis of SLC7A5, the most significant KEGG pathway was considerably enriched in spliceosome and pentose phosphate pathway.

Conclusion: We established a novel ceRNA regulatory network in TAD, which provides valuable information for further research in the molecular mechanisms of TAD.
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http://dx.doi.org/10.2147/IJGM.S335082DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8528547PMC
October 2021

Characteristic analysis of volatile avalanche diode threshold switching for bionic nerve synapse applications.

Sci Rep 2021 Oct 26;11(1):21051. Epub 2021 Oct 26.

Department of College of Mechatronic Engineering and Automation, School of Shanghai University, Shanghai, 200444, China.

The combination of biological neurology and memristive theory has greatly promoted the development of neuromorphic computing. To build a large-scale artificial intelligence alert system, the exploration of bionic synapses compatible with standard processes has become an urgent problem to be solved in the next step. In response to the above application requirements, this paper proposes a volatile avalanche diode threshold switching (VADTS) that is fully compatible with standard semiconductor technology to simulate the various functions of the synapse. Technology computer-aided design device-level simulation can verify the bionic principle of VADTS. The function of VADTS's bionic synapse was verified by the experimental test platform. The results show that under the action of the excitation signal (11.25 V), the device can continuously change from a high-resistance state to a low-resistance state. When the excitation signal is lower than the threshold, VADTS presents a "no adaptation" state of nerve synapses. When the excitation signal is higher than the threshold and changes continuously, the current changes along with the amplitude of the excitation signal, similar to the "sensitization" state of the nerve synapse.
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http://dx.doi.org/10.1038/s41598-021-00594-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8548554PMC
October 2021

Zwitterionic polymer modified xanthan gum with collagen II-binding capability for lubrication improvement and ROS scavenging.

Carbohydr Polym 2021 Nov 17;274:118672. Epub 2021 Sep 17.

College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, PR China; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China; Med-X Center for Materials, Sichuan University, Chengdu 610041, PR China. Electronic address:

High friction of damaged cartilage requires long-acting lubricated additive, which can also effectively scavenge reactive oxidative species (ROS) produced by mechanically stimulated chondrocytes. In this study, xanthan gum (XG) was grafted by poly (sulfobetaine methacrylate) (PSBMA) (the [XG]/[SBMA] molar ratio is 1:5 or 1:10), forming nanoparticles and then conjugated with collagen II-binding peptide, finally obtaining CBPXGSB1/5 or CBPXGSB1/10. Therein, the CBPXGSB1/5 was chosen as optimal lubricated additive. The results show that hydrated effect of PSBMA side chains endows CBPXGSB1/5 with favorable lubrication property (COF is 0.063). Furthermore, the CBPXGSB1/5 combining lubrication property and specific binding capability together may achieve the long-acting lubrication for injured cartilage in medical field. The CBPXGSB1/5 also possesses antioxidation verified by DPPH assay and exhibits synergistically enhanced ROS (OH, O and HO) scavenging. Besides, cytotoxicity experiment demonstrates that CBPXGSB1/5 has good biocompatibility. Therefore, multifunctional CBPXGSB1/5 developed here may have promising application potential in osteoarthritis treatment.
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http://dx.doi.org/10.1016/j.carbpol.2021.118672DOI Listing
November 2021

Identification and genome analysis of a novel picornavirus from captive belugas (Delphinapterus leucas) in China.

Sci Rep 2021 Oct 25;11(1):21018. Epub 2021 Oct 25.

Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, 571199, China.

The discovery of new viruses is important for predicting their potential threats to the health of humans and other animals. A novel picornavirus was identified from oral, throat, and anal swab samples collected from belugas (Delphinapterus leucas), from Dalian Sun Asia Tourism Holding Co., China, between January and December 2018, using a metagenomics approach. The genome of this novel PicoV-HMU-1 strain was 8197 nucleotides (nt) in length, with a open reading frame (from 1091 to 8074 nt) that encoded a polyprotein precursor of 2328 amino acids. Moreover, the genomic length and GC content of PicoV-HMU-1 were within the ranges found in other picornaviruses, and the genome organization was also similar. Nevertheless, PicoV-HMU-1 had a lower amino acid identity and distinct host species compared with other members of the Picornaviridae family. Phylogenetic trees were constructed based on the P1 and 3D amino acid sequences of PicoV-HMU-1 along with representative members of the Picornaviridae family, which showed that PicoV-HMU-1 was related to unclassified bat picornaviruses groups. These findings suggest that the PicoV-HMU-1 strain represents a potentially novel genus of picornavirus. These data can enhance our understanding of the picornavirus genetic diversity and evolution.
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http://dx.doi.org/10.1038/s41598-021-00605-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8549006PMC
October 2021

Highly Distorted Grain Boundary with an Enhanced Carrier/Phonon Segregation Effect Facilitates High-Performance Thermoelectric Materials.

ACS Appl Mater Interfaces 2021 Nov 26;13(43):51018-51027. Epub 2021 Oct 26.

School of Advanced Materials, Peking University Shenzhen Graduate School, Shenzhen 518055, China.

Grain boundary plays an important role in determining the phonon/carrier transport behaviors of thermoelectric (TE) materials, especially for the polycrystalline materials with the average grain size in nanoscale dimensions. Adjusting the grain size and boundaries of TE materials is considered as an effective approach to decouple TE parameters and thereby synergistically optimize the TE performance. Here, the highly distorted grain boundary with an enhanced carrier/phonon segregation effect is introduced to the commercial n-type BiTeSe matrix. The existence of highly distorted grain boundaries can not only enhance the interfacial Seebeck coefficient without significant detriment to the electrical conductivity but also increase the interfacial thermal resistance of grain boundaries, leading to the synergistic reduction of the thermal conductivity. As a result, a peak figure of merit ≈ [email protected] K and an average ≈ 1.1(300-500 K) are obtained, which are about 55 and 57% higher than the corresponding values of the commercial BiTeSe matrix, respectively. This work represents a new avenue for improving the commercial BiTeSe TE material, which could further promote the development of the TE technology.
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http://dx.doi.org/10.1021/acsami.1c15422DOI Listing
November 2021

Enhanced mobilization of Cd from commercial pigments in the rhizosphere of flooded lowland rice.

Sci Total Environ 2021 Oct 22:151032. Epub 2021 Oct 22.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China. Electronic address:

Although yellow Cd pigments (Cd-YP), widely used in industrial colorants, are considered inert, increasing evidence suggests once released into the environment, photobleaching/weathering mobilizes Cd from these pigments posing a pollution threat. Although general redox conditions and biotic/microbial activity are known to be important factors in determining Cd release, how spatial trends and specific soil processes regulate the Cd-YP behavior are poorly understood. Using plant rhizotrons in controlled environmental conditions, this study investigated the behavior of Cd-YP amendments matched to levels (15 mg kg) representative of contaminated soils in Yixing, China. Using high-resolution two-dimensional diffusive-gradient-in-thin-films (HR-2D-DGT), planar-optode (PO) multilayer systems alongside targeted soil and porewater sampling for chemical analysis the biogeochemistry associated with Cd mobilization from Cd-YP rice rhizospheres were determined. The results showed that there was a significant release of Cd into soil porewaters (51.5 μg L), but this reduced by 90.9% and stabilized over time (after 6-days). HR-2D-DGT ion-maps revealed pronounced spatial variances. The flux-maxima for Cd, which located within aerobic-rhizosphere zones, was 9 to 19-fold higher than in associated anoxic bulk soil. In general, zones of radial O loss (ROL)/higher redox conditions and lower pH were associated with Cd release, with S to SO transitions marking the boundaries of high-flux areas. Some isolated colocalization of Fe and Cd hotspots were observed in lateral root regions, but on-the-whole Fe/Mn and Cd release were not linked. In addition, microniche development was also an important feature of Cd mobilization due to soil heterogeneity.
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http://dx.doi.org/10.1016/j.scitotenv.2021.151032DOI Listing
October 2021

Comprehensive Characterization of Androgen-Responsive circRNAs in Prostate Cancer.

Life (Basel) 2021 Oct 15;11(10). Epub 2021 Oct 15.

Department of Urology, Shanghai Fourth People's Hospital Affiliated to Tongji University School of Medicine, Shanghai 200434, China.

The androgen receptor (AR) signaling pathway plays an important role in the initiation and progression of prostate cancer. Circular RNAs (circRNAs), the novel noncoding RNAs without 5' to 3' polarity or 3' poly (A), play an important role in multiple diseases. However, the potential roles of androgen-responsive circRNAs in prostate cancer remain unclear. In this study, we identified 3237 androgen-responsive circRNAs and 1954 androgen-responsive mRNAs after dihydrotestosterone (DHT) stimulation using microarray. Among them, the expression of 1296 androgen-responsive circRNAs was consistent with that of their parent genes, and we thought AR might regulate the expression of these circRNAs at the transcriptional level. In addition, 1941 circRNAs expression was not consistent with their parent genes, and we speculated that AR may regulate the expression of those circRNAs at the posttranscriptional level through affecting alternative splicing. Analyzing the androgen-responsive circRNAs regulated at the posttranscriptional level, we identified two key RNA binding proteins (RBPs), WTAP and TNRC6, using the circInteractome database, which may play important role in the biogenesis of androgen-responsive circRNAs. Furthermore, we explored the potential biological functions and predicted the molecular mechanisms of two dysregulated circRNAs ( and ) in prostate cancer. In this study, we revealed that was upregulated in prostate cancer tissues and plasma samples from patients with prostate cancer; may play an oncogenic role in prostate cancer. In contrast, was downregulated and may act as a tumor suppressor in prostate cancer. Our results suggest that androgen-responsive circRNAs might regulate the progression of prostate cancer and could be novel diagnostic biomarkers.
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http://dx.doi.org/10.3390/life11101096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538364PMC
October 2021

Camrelizumab for relapsed or refractory classical Hodgkin lymphoma: Extended follow-up of the multicenter, single-arm, Phase 2 study.

Int J Cancer 2021 Oct 21. Epub 2021 Oct 21.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Lymphoma, Peking University Cancer Hospital & Institute, Beijing, China.

Camrelizumab (a humanized high-affinity IgG4 mAb against programmed death-l) showed potent antitumor activity, well tolerance and controllable safety in patients with relapsed or refractory classical Hodgkin lymphoma (r/r cHL), based on the primary analysis of a Phase 2 study. Here, we present the extended follow-up outcomes. Seventy-five patients who had failed to achieve a remission or experienced progression after autologous stem cell transplantation or had received at least two lines of systemic chemotherapies were enrolled to receive camrelizumab 200 mg every 2 weeks. With a median follow-up of 36.2 months (range, 7.2-38.1), objective response rate per independent central review was 76.0% (95% confidence interval [CI], 64.7-85.1). Among the 57 responders, 31 (54.4%) had ongoing responses. Median duration of response was 31.7 months (95% CI, 16.7-not reached). Median progression-free survival was 22.5 months (95% CI, 14.7-not reached). Thirty-six-month overall survival rate was 82.7% (95% CI, 72.0-89.5). Reactive capillary endothelial proliferation (RCEP) occurred in 97.3% of patients (73/75), but all RCEP were Grade 1 or 2 in severity and 67.1% of these patients (49/73) achieved complete resolution. Occurrence of new RCEP lesions was rare (8/42 [19.0%] at 12 months; 2/32 [6.3%] at 24 months). No treatment-related deaths occurred, and no new toxicities were reported. With extended follow-up, camrelizumab monotherapy continues to provide a robust and durable response, long survival and manageable safety in r/r cHL patients.
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http://dx.doi.org/10.1002/ijc.33852DOI Listing
October 2021

Atypical choroid plexus papilloma: A case report.

Authors:
Yanyan Chen Jun Luo

Asian J Surg 2021 Oct 11. Epub 2021 Oct 11.

Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, Hubei, China. Electronic address:

We presents a rare case of a 15-year-old female who suffered atypical choroid plexus papilloma, which was accidental discovery due to head trauma. We gives a detailed description of its histological morphology and summarizes its clinical, pathological and prognostic characteristics, which is helpful for clinicians and pathologists to have a better understanding of this new disease.
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http://dx.doi.org/10.1016/j.asjsur.2021.09.011DOI Listing
October 2021

Marek's disease virus encoded miR-M6 and miR-M10 are dispensable for virus replication and pathogenesis in chickens.

Vet Microbiol 2021 Nov 5;262:109248. Epub 2021 Oct 5.

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, Henan, People's Republic of China; International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, Henan, People's Republic of China. Electronic address:

MicroRNAs (miRNAs) are a class of approximately 22 nucleotides long non-coding RNAs, and virus-encoded miRNAs play an important role in pathogenesis. Marek's disease virus (MDV) is an oncogenic avian alphaherpesvirus that causes immunosuppression and tumors in its natural host, chicken. In the MDV genome, 14 miRNA precursors and 26 mature miRNAs were identified, thus MDV has been used as a model to study the function of viral miRNAs in vivo. Recently, a cluster of miRNAs encoded by MDV, Cluster 3 miRNAs (miR-M8-M10), has been shown to restrict early cytolytic replication and pathogenesis of MDV. In this study, we further analyzed the role of miR-M6 and miR-M10, members of cluster miR-M8-M10, in MDV replication and pathogenicity. We found that, compared to parental MDV, deletion of miR-M6-5p significantly enhanced the replication of MDV in cell culture, but not in chickens. The replication of miR-M6-5p deletion MDV was restored once the deleted sequences were re-inserted. Our results also showed that deletion of miR-M10-5p did not affect the replication of MDV in vitro and in vivo. In addition, our animal study results showed that deletion of miR-M6-5p or miR-M10-5p did not alter the pathogenesis of MDV. In conclusion, our study shows that both miR-M6 and miR-M10 are dispensable for MDV replication and pathogenesis in chickens, while also suggests a repressive role of miR-M6 in MDV replication in cell culture.
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http://dx.doi.org/10.1016/j.vetmic.2021.109248DOI Listing
November 2021

Dissecting the molecular mechanism of russeting in sand pear (Pyrus pyrifolia Nakai) by metabolomics, transcriptomics, and proteomics.

Plant J 2021 Oct 8. Epub 2021 Oct 8.

Forest & Fruit Tree Research Institute, Shanghai Academy of Agriculture Sciences, Shanghai, 201403, China.

Brown coloration and a rough appearance as russet and semi-russet (partial russet) are features unique to the popular Asian sand pear (Pyrus pyrifolia Nakai). The degree of russeting is different between different genotypes. Russeting is sensitive to water fluctuations, where excessive rainwater can trigger/stimulate its development. However, the molecular mechanism of russeting is currently unclear. Here, we employed multi-omics, i.e., metabolomics, transcriptomics, and proteomics, and analyzed the effect of different sand pear genotypes and artificial rainfall on russeting of pear fruits. This led to the identification of 79, 64, and 29 differentially produced/expressed metabolites, transcripts, and proteins that are involved in the biosynthesis of suberin, phenylpropane, cutin, and waxes. Further analysis of these differentially expressed genes and their encoded proteins revealed that four of them exhibited high expression at both transcript and protein levels. Transient expression of one such gene, PbHHT1 (accession number 103966555), which encodes ω-hydroxypalmitate-O-feruloyl transferase, in young green non-russet fruits triggered premature suberization in the russeting pear genotypes. This coincided with increased production of 16-feruloyloxypalmitic acid, a conjugated compound between phenols and esters during the polymerization for suberin formation. Collectively, our data from the combined three omics demonstrate that russeting in sand pear is a complex process involving the biosynthesis and transport of suberin and many other secondary metabolites.
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http://dx.doi.org/10.1111/tpj.15532DOI Listing
October 2021

Crystal structures of human coronavirus NL63 main protease at different pH values.

Acta Crystallogr F Struct Biol Commun 2021 Oct 27;77(Pt 10):348-355. Epub 2021 Sep 27.

School of Basic Medical Sciences, Nanchang University, Nanchang, Jiangxi 330031, People's Republic of China.

Human coronavirus NL63 (HCoV-NL63), which belongs to the genus Alphacoronavirus, mainly infects children and the immunocompromized and is responsible for a series of clinical manifestations, including cough, fever, rhinorrhoea, bronchiolitis and croup. HCoV-NL63, which was first isolated from a seven-month-old child in 2004, has led to infections worldwide and accounts for 10% of all respiratory illnesses caused by etiological agents. However, effective antivirals against HCoV-NL63 infection are currently unavailable. The HCoV-NL63 main protease (M), also called 3C-like protease (3CL), plays a vital role in mediating viral replication and transcription by catalyzing the cleavage of replicase polyproteins (pp1a and pp1ab) into functional subunits. Moreover, M is highly conserved among all coronaviruses, thus making it a prominent drug target for antiviral therapy. Here, four crystal structures of HCoV-NL63 M in the apo form at different pH values are reported at resolutions of up to 1.78 Å. Comparison with M from other human betacoronaviruses such as SARS-CoV-2 and SARS-CoV reveals common and distinct structural features in different genera and extends knowledge of the diversity, function and evolution of coronaviruses.
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http://dx.doi.org/10.1107/S2053230X21009523DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8488857PMC
October 2021

Dynamic metabolic and transcriptomic profiling reveals the biosynthetic characteristics of hydroxycinnamic acid amides (HCAAs) in sunflower pollen.

Food Res Int 2021 11 31;149:110678. Epub 2021 Aug 31.

State Key Laboratory of Natural Medicines and Jiangsu Key of Bioactive Natural Product Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 210009, People's Republic of China. Electronic address:

Sunflower pollen is a natural nutritious food with a long history and multiple functions, however, the main chemical components apart from flavonoids and their biosynthesis processes have not been thoroughly investigated. In this study, seven hydroxycinnamic acid amides (HCAAs) (1-7) abundant in sunflower pollen were isolated and identified as one type of the pollen's main chemicals. For a comprehensive understanding of HCAA biosynthesis in Helianthus annuus flowers, RNA-seq, metabolomics, and key genes related to biosynthesis in the sunflower were studied. A large number of compounds at different sunflower growth stages (the 7th, 14th, 21st, and 28th days) and high expression levels of related genes in the transcriptome were detected. A molecular network was constructed to clarify the synthetic pathway of HCAAs, which revealed high transcriptional levels of spermidine hydroxycinnamoyl transferase genes (HaSHT2795 and HaSHT2436) in 14-21-days-old flowers. HaSHT2795 enzymes catalyze tri-coumaroylspermidine formation, and virus-induced gene silencing to inhibit HaSHT2795 and HaSHT2436 could significantly reduce the synthesis of hydroxycinnamic acid amides in sunflower pollen. HCAAs were inferred to be related to the formation of pollen walls and the health effects of pollen. Analyzing HCAA biosynthesis and accumulation in H. annuus pollen will be helpful to understand the functions of HCAAs in the development of pollen and its nutritional value.
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http://dx.doi.org/10.1016/j.foodres.2021.110678DOI Listing
November 2021

Heterogenous hydrogel mimicking the osteochondral ECM applied to tissue regeneration.

J Mater Chem B 2021 Oct 27;9(41):8646-8658. Epub 2021 Oct 27.

College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu 610065, China.

Inspired by the intricate extracellular matrix (ECM) of natural cartilage and subchondral bone, a heterogenous bilayer hydrogel scaffold is fabricated. Gelatin methacrylate (GelMA) and acryloyl glucosamine (AGA) serve as the main components in the upper layer, mimicking the chondral ECM. Meanwhile, vinylphosphonic acid (VPA) as a non-collagen protein analogue is incorporated into the bottom layer to induce the biomineralization of calcium phosphate. The two heterogenous layers are effectively sutured together by the inter-diffusion between the upper and bottom layer hydrogels, together with chelation between the calcium ions and alginate added to separate layers. The interfacial bonding between the two different layers was thoroughly investigated rheological measurements. The incorporation of AGA promotes chondrocytes to produce collagen type II and glycosaminoglycans and upregulates the expression of chondrogenesis-related genes. In addition, the minerals induced by VPA facilitate the osteogenesis of bone marrow mesenchymal stem cells (BMSCs). evaluation confirms the biocompatibility of the scaffold with minor inflammation and confirms the best repair ability of the bilayer hydrogel. This cell-free, cost-effective and efficient hydrogel shows great potential for osteochondral repair and inspires the design of other tissue-engineering scaffolds.
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http://dx.doi.org/10.1039/d1tb00518aDOI Listing
October 2021

Structure-based discovery and structural basis of a novel broad-spectrum natural product against main protease of coronavirus.

J Virol 2021 Sep 29:JVI0125321. Epub 2021 Sep 29.

Institute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen 518112, Guangdong Province, China.

Over the past 20 years, Severe acute respiratory syndrome CoV (SARS-CoV), Middle East respiratory syndrome CoV (MERS-CoV) and severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), have all emerged, causing severe epidemic human respiratory diseases throughout the globe. Developing broad spectrum drugs would be invaluable in responding to new emerging coronaviruses of the future and could address unmet urgent clinical needs. Main protease (M, also known as 3CL) has a major role in the replication of a coronavirus life cycle and is one of the most important drug targets for anticoronavirus agents. We show that a natural product, noncovalent inhibitor shikonin, can pan-main protease inhibitor of SARS-CoV-2, SARS-CoV, MERS-CoV, HCoV-HKU1, HCoV-NL63 and HCoV-229E with micromolar IC values. Structures of the main protease of different coronavirus genus, SARS-CoV from betacoronaviruses and HCoV-NL63 from alphacoronaviruses, were determined by X-ray crystallography and reveals that the inhibitor interacts with key active-site residues in a unique mode. The structure of the main protease inhibitor complex presents an opportunity to discover a novel series of broad-spectrum inhibitors. These data provide substantial evidence that shikonin and its derivatives may be effective against most coronaviruses, as well as emerging coronaviruses in the future. Given the importance of main protease for the coronavirus therapeutic indication, insights from these studies should accelerate the development and design of safer and more effective antiviral agents. The current pandemic has created an urgent need for broad spectrum inhibitors of SARS-CoV-2. Main protease is relatively conservative compared with the spike protein and thus is one of the most promising drug targets for developing anticoronavirus agents. We have solved crystal structures of main protease of SARS-CoV and HCoV-NL63 bound to shikonin. The structures provide important insights that have broad implications for understanding the structural basis underlying enzyme activity, and can facilitate rational design of broad spectrum anticoronavirus ligands as new therapeutic agents.
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http://dx.doi.org/10.1128/JVI.01253-21DOI Listing
September 2021

Genome-wide identification of soybean Shaker K channel gene family and functional characterization of GmAKT1 in transgenic Arabidopsis thaliana under salt and drought stress.

J Plant Physiol 2021 Nov 22;266:153529. Epub 2021 Sep 22.

College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Jilin, 130118, China; College of Tropical Crops, Hainan University, Haikou, 570228, China. Electronic address:

Potassium is a major cationic nutrient involved in numerous physiological processes in plants. The uptake of K is mediated by K channels and transporters, and the Shaker K channel gene family plays an essential role in K uptake and stress resistance in plants. However, little is known regarding this family in soybean. In this study, 14 members of the Shaker K channel gene family were identified in soybean and were classified into five groups. Protein domain analysis revealed that Shaker K channel gene members have an ion transport domain (ion trans), a cyclic nucleotide-binding domain, ankyrin repeat domains, and a dimerization domain in the potassium ion channel. Quantitative real-time polymerase chain reaction analysis indicated that the expression of eight genes (notably GmAKT1) in soybean leaves and roots was significantly increased in response to salt and drought stress. Furthermore, the overexpression of GmAKT1 in Arabidopsis enhanced root length, K concentration, and fresh/dry weight ratio compared with wild-type plants subjected to salt and drought stress; this suggests that GmAKT1 improves the tolerance of soybean to abiotic stress. Our results provide important insight into the characterization of Shaker K channel gene family members in soybean and highlight the function of GmAKT1 in soybean plants under salt and drought stress.
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http://dx.doi.org/10.1016/j.jplph.2021.153529DOI Listing
November 2021

Direct Fabrication of Micron-Thickness PVA-CNT Patterned Films by Integrating Micro-Pen Writing of PVA Films and Drop-on-Demand Printing of CNT Micropatterns.

Nanomaterials (Basel) 2021 Sep 8;11(9). Epub 2021 Sep 8.

School of Mechanical Engineering, Northwestern Polytechnical University, Xi'an 710072, China.

The direct fabrication of micron-thickness patterned electronics consisting of patterned PVA films and CNT micropatterns still faces considerable challenges. Here, we demonstrated the integrated fabrication of PVA films of micron-thickness and CNT-based patterns by utilising micro-pen writing and drop-on-demand printing in sequence. Patterned PVA films of 1-5 μm in thickness were written first using proper micro-pen writing parameters, including the writing gap, the substrate moving velocity, and the working pressure. Then, CNT droplets were printed on PVA films that were cured at 55-65 °C for 3-15 min, resulting in neat CNT patterns. In addition, an inertia-pseudopartial wetting spreading model was established to release the dynamics of the droplet spreading process over thin viscoelastic films. Uniform and dense CNT lines with a porosity of 2.2% were printed on PVA substrates that were preprocessed at 55 °C for 9 min using a staggered overwriting method with the proper number of layers. Finally, we demonstrated the feasibility of this hybrid printing method by printing a patterned PVA-CNT film and a micro-ribbon. This study provides a valid method for directly fabricating micron-thickness PVA-CNT electronics. The proposed method can also provide guidance on the direct writing of other high-molecular polymer materials and printing inks of other nanosuspensions.
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http://dx.doi.org/10.3390/nano11092335DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466232PMC
September 2021

Importance of water content in birnessite-type MnO catalysts for HCHO oxidation: Mechanistic details and DFT analysis.

Chemosphere 2022 Jan 18;287(Pt 3):132293. Epub 2021 Sep 18.

School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan, 410083, China.

Water is featured in an indispensable role during the process of catalytic oxidation of HCHO. In this work, a rich water-containing birnessite-type MnO was synthesized, and its water content was adjusted through calcination. Phase structure and texture properties of the prepared birnessite were characterized. It was revealed that three types of water (namely absorbed water, molecular water, and structural hydroxyl) existed in birnessite. With the loss of water content, the interlayer distance of samples had decreased which changed the structure of birnessite to cryptomelane. This converted the morphology from an initial layered shape to a rod-like shape. Besides, the underlying mechanism for this effect on HCHO catalytic oxidation was elucidated. Results indicated that hydroxyl groups could slowly and sequentially oxidize HCHO to DOM, formate, and carbonate species. The hydroxyl groups also promoted the formation of oxygen vacancy which could activate O to O- 2 and O. The hydroxyl groups which were consumed had originally been supplied by the reaction between O- 2, O and HO (absorbed and interlayer water in birnessite) which was then replenished from air stream. Clearly, water is favorable to the catalytic reaction. It is the main reason why birnessite can continuously decompose HCHO.
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http://dx.doi.org/10.1016/j.chemosphere.2021.132293DOI Listing
January 2022

The blood pressure control and arteriosclerotic cardiovascular risk among Chinese community hypertensive patients.

Sci Rep 2021 Sep 24;11(1):19066. Epub 2021 Sep 24.

Department of Chronic and Non-Infection Disease Control and Prevention, Hangzhou Center for Disease Control and Prevention, Mingshi Road No.568, Hangzhou, 310021, China.

The present study aimed to describe the blood pressure (BP) control rate and 10-years arteriosclerotic cardiovascular disease (ASCVD) risk estimation among community hypertensive patients. A total of 196,803 subjects were enrolled. The control rates calculated as the intensive (SBP < 130 mmHg and DBP < 80 mmHg) and standard (SBP < 140 mmHg and DBP < 90 mmHg) threshold. Multivariable logistic analysis was employed to assess the associations between cardiovascular factors and BP control. Sensitivity, specificity and Youden's index were used to identify the ability of high risk of ASCVD estimation by different thresholds. The control rate was 16.34% and 50.25% by the intensive and standard threshold, respectively. Besides regular medication, the risk factors for BP control included older age, male, unhealthy lifestyle, obesity, dyslipidemia and abnormal FPG. 25.08% of subjects had high risk of 10-years ASCVD estimation. The sensitivity, specificity and Youden's index of intensive threshold was 84.37%, 16.15% and 0.51%, and were significantly different from 50.55%, 50.42% and 0.98% of the standard threshold, respectively. Half of community hypertensive patients did not control BP, and nearly a quarter have high risk of 10-years ASCVD risk estimation. The intensive threshold resulted in a one-third reduction in the control rate compared to the standard threshold. No matter which threshold was used, a single BP control status seemed not a suitable indicator for identification of high risk of 10-years ASCVD risk estimation.
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http://dx.doi.org/10.1038/s41598-021-98745-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8463712PMC
September 2021

Lignans with (N, N-diethyl)methyl amino group from Buxus rugulosa.

Chin J Nat Med 2021 Sep;19(9):675-679

Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 210009, China. Electronic address:

Buxrugulosides A-E, four lignan glycosides (1-4) and a protocatechuate derivative (5) featuring a rare (N, N-diethyl)methyl amino group at aromatic rings, were obtained from the aerial parts of Buxus rugulosa, which is famous for treating coronary heart disease. Their structures including absolute configurations were elucidated by HRMS, 1D and 2D NMR, and by comparing their CD data with previous reports. Compound 1 was a rare sesquilignan, and all of these compounds were the first example of lignans with (N, N-diethyl)methyl amino group.
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http://dx.doi.org/10.1016/S1875-5364(21)60064-XDOI Listing
September 2021

Combining Multiple High-Resolution Techniques to Understand Phosphorous Availability Around Rice Roots.

Environ Sci Technol 2021 10 23;55(19):13082-13092. Epub 2021 Sep 23.

State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, Jiangsu 210023, China.

Resolving chemical/biological drivers of P behavior around lowland/flooded rice roots remains a challenge because of the heterogeneity of the plant-soil interactions, compounded by sampling and analytical constraints. High-spatial-resolution (sub-mm) visualization enables these processes to be isolated, characterized, and deciphered. Here, three advanced soil imaging systems, diffusive gradients in thin-film technique coupled with laser ablation-ICPMS (DGT-LA-ICPMS), O planar optode, and soil zymography, were integrated. This trio of approaches was then applied to a rice life cycle study to quantify solute-P supply, through two dimensions, , and low-disturbance high-resolution (HR) chemical imaging. This allowed mechanisms of P release to be delineated by O, Fe, and phosphatase activity mapping at the same scale. HR-DGT revealed P depletion around both living and dead rice roots but with highly spatially variable Fe/P ratios (∼0.2-12.0) which aligned with changing redox conditions and root activities. Partnering of HR-DGT and soil zymography revealed concurrent P depletion and phosphatase hotspots in the rhizosphere and detritusphere zones (Mantel: 0.610-0.810, < 0.01). This close affinity between these responses (Pearson correlation: -0.265 to -0.660, < 0.01) cross-validates the measurements and reaffirms that P depletion stimulates phosphatase activity and P mineralization. The μ-scale biogeochemical landscape of rice rhizospheres and detritusphere, as documented here, needs greater consideration when implementing interventions to improve sustainable P nutrition.
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http://dx.doi.org/10.1021/acs.est.1c05358DOI Listing
October 2021

Mussel-inspired self-assembly engineered implant coatings for synergistic anti-infection and osteogenesis acceleration.

J Mater Chem B 2021 Oct 20;9(40):8501-8511. Epub 2021 Oct 20.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.

Implant associated infections (IAI) and poor osseointegration are the two major causes for titanium implant failure, leading to subsequent financial burden and physical sufferings. Therefore, advanced implants with excellent anti-infection and osseointegration performance are needed. In this work, mussel-inspired tannic acid (TA) mediated layer-by-layer (LbL) self-assembly was used for fabricating bonded polyethylene glycol (PEG) and 8DSS (8 repeating units of aspartate-serine-serine) coatings (Ti/8DSS/PEG) on the surface of titanium implants. The coating is designed to simultaneously reduce bacterial adhesion through the super-hydrophilic effect of PEG and promote osseointegration through the effective biomineralization of 8DSS. The obtained Ti/8DSS/PEG implant exhibits superior anti-biofouling capabilities (anti-protein adhesion and anti-bacterial adhesion against and ) and excellent biocompatibility. Meanwhile, the Ti/8DSS/PEG implant accelerates osteoblast differentiation and presents significantly better osteogenic ability than bare titanium implants . This mussel-inspired TA mediated LbL self-assembly method is expected to provide a multifunctional and robust platform for surface engineering in bone repair.
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http://dx.doi.org/10.1039/d1tb01607eDOI Listing
October 2021

Rutin inhibited the advanced glycation end products-stimulated inflammatory response and extra-cellular matrix degeneration via targeting TRAF-6 and BCL-2 proteins in mouse model of osteoarthritis.

Aging (Albany NY) 2021 09 22;13(18):22134-22147. Epub 2021 Sep 22.

Department of Rehabilitation Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang 330000, Jiangxi Province, China.

Background: Osteoarthritis (OA) is degenerative joint disorder mainly characterized by long-term pain with limited activity of joints, the disease has no effective preventative therapy. Rutin (RUT) is a flavonoid compound, present naturally. The flavonoid shows range of biological activities such as anti-inflammatory and anti-cancer effect. We screened RUT for its activity against osteoarthritis with and models of osteoarthritis.

Methods: Animal model of OA was developed using C57BL/6 mice by surgical destabilization of medial meniscus. For studies the human articular cartilage tissues were used which were collected from osteoarthritis patients and were processed to isolate chondrocytes. The chondrocytes were submitted to advanced glycation end products (AGEs) for inducing osteoarthritis . Cell viability was done by CCK-8 assay, ELISA analysis for MMP13, collage II, PGE2, IL-6, TNF-α, ADAMTS-5 and MMP-13. Western blot analysis was done for expression of proteins and analysis was done by docking studies.

Results: Pretreatment of RT showed no cytotoxic effect and also ameliorated the AGE mediated inflammatory reaction on human chondrocytes . Treatment of RT inhibited the levels of COX-2 and iNOS in AGE exposed chondrocytes. RT decreased the AGE mediated up-regulation of IL-6, NO, TNF-α and PGE-2 in a dose dependent manner. Pretreatment of RT decreased the extracellular matrix degradation, inhibited expression of TRAF-6 and BCL-2 the NF-κB/MAPK pathway proteins. The treatment of RT in mice prevented the calcification of cartilage tissues, loss of proteoglycans and also halted the narrowing of joint space is mice subjected to osteoarthritis. The analysis suggested potential binding affinity of RT with TRAF-6 and BCL-2.

Conclusion: In brief RT inhibited AGE-induced inflammatory reaction and also degradation of ECM via targeting the NF-κB/MAPK pathway proteins BCL-2 and TRAF-6. RT can be a potential molecule in treating OA.
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http://dx.doi.org/10.18632/aging.203470DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8507296PMC
September 2021
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