Publications by authors named "Jun Li"

10,208 Publications

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Research progress in external field intensification of forward osmosis process for water treatment: A critical review.

Water Res 2022 Aug 2;222:118943. Epub 2022 Aug 2.

College of Environmental Science and Engineering, Key Laboratory of Science & Technology of Eco-Textile, Ministry of Education, Donghua University, Shanghai 201620, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China; State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai 201620, China. Electronic address:

Forward osmosis (FO) is an emerging permeation-driven membrane technology that manifests advantages of low energy consumption, low operating pressure, and uncomplicated engineering compared to conventional membrane processes. The key issues that need to be addressed in FO are membrane fouling, concentration polarization (CP) and reverse solute diffusion (RSD). They can lead to problems about loss of draw solutes and reduced membrane lifetime, which not only affect the water treatment effectiveness of FO membranes, but also increase the economic cost. Current research has focused on FO membrane preparation and modification strategies, as well as on the selection of draw solutions. Unfortunately, these intrinsic solutions had limited success in unraveling these phenomena. In this paper, we provide a brief review of the current state of research on existing external field-assisted FO systems (including electric-, pressure-, magnetic-, ultrasonic-, light- and flow-assisted FO system), analyze their mitigation mechanisms for the above key problems, and explore potential research directions to aid in the further development of FO systems. This review aims to reveal the feasibility of the development of external field-assisted FO technology to achieve a more economical and efficient FO treatment process.
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http://dx.doi.org/10.1016/j.watres.2022.118943DOI Listing
August 2022

Effect of Tetrahedral Framework Nucleic Acids on Neurological Recovery via Ameliorating Apoptosis and Regulating the Activation and Polarization of Astrocytes in Ischemic Stroke.

ACS Appl Mater Interfaces 2022 Aug 11. Epub 2022 Aug 11.

State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, P. R. China.

Astrocytes, as the most plentiful subtypes of glial cells, play an essential biphasic function in ischemic stroke (IS). However, although having beneficial effects on stroke via promoting nerve restoration and limiting lesion extension, astrocytes can unavoidably cause exacerbated brain damage due to their participation in the inflammatory response. Therefore, seeking an effective and safe drug/strategy for protecting and regulating astrocytes in stroke is urgent. Here, we employ tetrahedral framework nucleic acid (tFNA) nanomaterials for astrocytes in stroke, considering their excellent biological properties and outstanding biosafety. In vitro, tFNA can inhibit calcium overload and ROS regeneration triggered by oxygen-glucose deprivation/reoxygenation (OGD/R), which provides a protective effect against astrocytic apoptosis. Furthermore, morphological changes such as hyperplasia and hypertrophy of reactive astrocytes are restrained, and the astrocytic polarization from the proinflammatory A1 phenotype to the neuroprotective A2 phenotype is facilitated by tFNA, which further alleviates cerebral infarct volume and facilitates the recovery of neurological function in transient middle cerebral artery occlusion (tMCAo) rat models. Moreover, the TLRs/NF-κB signaling pathway is downregulated by tFNA, which may be the potential mechanism of tFNA for protecting astrocytes in stroke. Collectively, we demonstrate that tFNA can effectively mediate astrocytic apoptosis, activation, and polarization to alleviate brain injury, which represents a potential intervention strategy for IS.
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http://dx.doi.org/10.1021/acsami.2c10364DOI Listing
August 2022

Identifying pancreatic cancer-associated miRNAs using weighted gene co-expression network analysis.

Oncol Lett 2022 Sep 5;24(3):297. Epub 2022 Jul 5.

Department of General Surgery, Shanxi Tumor Hospital, Taiyuan, Shanxi 030000, P.R. China.

Pancreatic cancer is a common type of gastrointestinal tumour throughout the world and is characterised by high malignancy rates and poor prognosis. Studies indicated that early and effective diagnosis is key to prolonging patients' overall survival, particularly in the case of fluid biopsy. Given this, the present study was designed to evaluate the expression profile arrays of patients with pancreatic cancer from the Gene Expression Omnibus database in an effort to identify differentially expressed microRNAs (miRNAs/miRs) that may be suitable for application in liquid biopsy-based diagnostics. Suitable miRNA candidates were identified using a weighted correlation network analysis (WGCNA) and key differentially expressed miRNAs were verified using reverse transcription-quantitative PCR. WGCNA identified 11 differentially expressed miRNAs (miR-155-5p, miR-4668-5p, miR-3613-3p, miR-3201, miR-548ac, miR-486-5p, miR-548a-3p, miR-8084, miR-455-3p, miR-6068 and miR-1246). Of these, miR-4668-5p was indicated to have the highest number of associated modules, making it most likely to be of diagnostic value. Thus, the present analysis identified 11 miRNAs associated with pancreatic cancer and further identified miR-4668-5p as a potential biomarker for pancreatic cancer diagnosis.
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http://dx.doi.org/10.3892/ol.2022.13417DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353221PMC
September 2022

Protective effect of inhibiting necroptosis on gentamicin-induced nephrotoxicity.

FASEB J 2022 Sep;36(9):e22487

Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, Anhui Institute of Innovative Drugs, School of Pharmacy, Anhui Medical University, Hefei, China.

Necroptosis is defined as a novel programmed cell necrosis that is mediated by receptor interacting serine-threonine protein kinase 1 (RIPK1) and other related signals. Necrosis, apoptosis and inflammation are commonly considered as the leading mechanism in acute kidney injury (AKI) induced by gentamicin (GEN), which is a useful antibiotic for treating the infection of Gram-negative bacterial. However, the necroptosis in the pathogenesis of GEN-induced AKI is unknown. In this study, to investigate the process and function of necroptosis in GEN-induced AKI, NRK-52E and HK-2 cells and SD rats were used as the models. The necroptosis-related proteins, including RIPK1, RIPK3, mixed lineage kinase domain-like (MLKL) and phosphorylated MLKL (p-MLKL), were all increasing time-dependently when GEN was continuously given. By using the RIPK1 inhibitor necrostatin-1 (NEC-1) and RIPK3 inhibitor (CPD42), the GEN-induced toxicity of tubular cells was alleviated. Moreover, it was validated that GEN-induced cell apoptosis and inflammation were attenuated after treating with NEC-1 or CPD42, both in vivo and in vitro. When MLKL was knocked down by siRNA, NEC-1 and CPD42 can not further protect the damage of tubular cells by GEN. Although the using of pan-caspase inhibitor Z-VAD significantly decreased GEN-induced apoptosis, it enhanced necroptosis and slightly promoted the decreased cell viability in GEN-treated cells, with the protective effects weaker than NEC-1 or CPD42. Finally, in vitro minimum inhibitory concentration (MIC) tests and bacteriostatic ring studies showed that NEC-1 did not interfere with the antibiotic effects of GEN. Thus, suppressing necroptosis can serve as a promising strategy for the prevention of GEN-induced nephrotoxicity.
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http://dx.doi.org/10.1096/fj.202200163RDOI Listing
September 2022

Shared Nearest Neighbors Approach and Interactive Browser for Network Analysis of a Comprehensive Non-Small-Cell Lung Cancer Data Set.

JCO Clin Cancer Inform 2022 Jul;6:e2200040

Department of Thoracic/Head and Neck Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX.

Purpose: Advances in biological measurement technologies are enabling large-scale studies of patient cohorts across multiple omics platforms. Holistic analysis of these data can generate actionable insights for translational research and necessitate new approaches for data integration and mining.

Methods: We present a novel approach for integrating data across platforms on the basis of the shared nearest neighbors algorithm and use it to create a network of multiplatform data from the immunogenomic profiling of non-small-cell lung cancer project.

Results: Benchmarking demonstrates that the shared nearest neighbors-based network approach outperforms a traditional gene-gene network in capturing established interactions while providing new ones on the basis of the interplay between measurements from different platforms. When used to examine patient characteristics of interest, our approach provided signatures associated with and new leads related to recurrence and TP53 oncogenotype.

Conclusion: The network developed offers an unprecedented, holistic view into immunogenomic profiling of non-small-cell lung cancer, which can be explored through the accompanying interactive browser that we built.
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http://dx.doi.org/10.1200/CCI.22.00040DOI Listing
July 2022

Relationship between morning peak phenomenon and early renal injury NGAL in H-type hypertension.

Blood Press 2022 Dec;31(1):200-206

Department of General Practice, Affiliated Hospital of Chengde Medical College, Chengde, China.

Purpose: To investigate the relationship between morning blood pressure surge (MBPS) and neutrophilgelatinase associated lipocalin (NGAL) in patients with H-type hypertension.

Materials And Methods: A total of 224 patients with diagnosed H-type hypertension [homocysteine (Hcy)≧10umol/L] were selected and underwent 24-hour ambulatory blood pressure monitoring (ABPM). In the morning peak group (115 cases), NGAL and serum cystatin C levels, -microglobulin levels were detected in each group, and general biochemical indicators were also detected.

Results: There was no significant difference in the course of hypertension, age, blood glucose, blood lipids, Hcy, BUN, Cr, and UA between the two groups ( > 0.05). CysC, -MG were higher than those in the nonmorning peak group, and the difference was statistically significant ( < 0.05).; Pearson correlation analysis showed that NGAL was moderately and highly correlated with CysC, systolic blood pressure morning peak, -MG, and high ( < 0.05), low-density lipoprotein (LDL-C), and Hcy were lowly correlated ( < 0.05).) and morning peak diastolic blood pressure ( > 0.05); multiple linear stepwise regression analysis indicated that morning peak systolic blood pressure, CysC,-MG, and FBG were the risk factors for NGAL.

Conclusion: The morning peak of systolic blood pressure in H-type hypertension is an important factor causing kidney injury. Paying attention to the ambulatory blood pressure monitoring and the control of morning peak blood pressure in patients with H-type hypertension, and early screening of NGAL has important clinical significance for the early prevention and treatment of renal injury in patients with H-type hypertension. PLAIN LANGUAGE SUMMARYThe morning peak of blood pressure is closely related to target organ damage.There are few studies on the relationship between morning peak phenomenon and renal damage in patients with H-type hypertension at home and abroad.We investigated the relationship between MBPS and NGAL in H-type hypertensive patients with BUN, Cr and UA in the normal range to provide a clinical basis for early renal protection in hypertensive patients.
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http://dx.doi.org/10.1080/08037051.2022.2106186DOI Listing
December 2022

Incidence and risk factors of delayed wound healing in patients who underwent unicompartmental knee arthroplasty.

Int Wound J 2022 Aug 8. Epub 2022 Aug 8.

Department of Orthopedic Surgery, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei, PR China.

Unicompartmental knee arthroplasty (UKA) has been proven as an ideal alternative surgical procedure to treat symptomatic isolated knee osteoarthritis, and recently this technique has gained its popularity. However, postoperative complications would inevitably compromise the effectiveness and patients' satisfaction. The objective of this study is to demonstrate the incidence and risk factors of delayed wound healing (DWH) after UKA. This retrospective cohort study was conducted from February 2021 to May 2022 and a total of 211 patients were enrolled. Demographic characteristics, operation-related variables, and laboratory indexes were extracted. Receiver operating characteristic analysis was performed to detect the optimum cut-off value for continuous variables. Univariate and multivariate logistic regression analysis was performed to demonstrate the risk factors of DWH. There were 155 female and 56 male patients with an average age of 64. 6 ± 6.9 years included in this study. After 6.6 ± 4.9 months' follow-up, 12 cases of DWH were observed which indicated an incidence of DWH of 5.7%, mean wound healing duration for 12 patients was 43.1 ± 19.3 days. In the univariate analysis, age > 62.5 years, postoperative hospital stay < 5.5 days, surgical incision < 10.5 cm, barbed suture, body mass index (BMI) > 32.0 kg/m , operation duration > 102.5 minutes, intraoperative blood loss > 102.5 mL, preoperative white blood cell count > 5.95*10 /L, preoperative seroglobulin (GLB) > 29.6 g/L, postoperative total protein < 63.4 g/L, postoperative serum albumin < 36.4 g/L, and postoperative GLB > 26.8 g/L were significantly different between patients with and without DWH (P < .05). In final multivariate logistic analysis, results showed that intraoperative blood loss > 102.5 mL (odds ratio [OR], 3.09; P = .001), postoperative hospital stay < 5.5 days (OR, 1.74; P = .014), surgical incision < 10.5 cm (OR, 1.67; P = .000), and BMI > 32.0 kg/m (OR, 4.47; P = .022) were independent risk factors for DWH. DWH prolongs hospital stay in UKA patients and increases healthcare expenditure; also affected the implementation schedule of postoperative functional exercise plans. Surgeons should identify patients at risk, meanwhile, make timely and correct clinical interventions to decrease the incidence of this complication.
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http://dx.doi.org/10.1111/iwj.13898DOI Listing
August 2022

sp. nov., with plant growth-promoting potential isolated from field soil after seven-years continuous maize cropping.

Int J Syst Evol Microbiol 2022 Aug;72(8)

Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.

A novel Gram-stain-positive, aerobic, non-motile and rod-shaped bacterium, designated strain NC76-1, was isolated from soil from a field that had undergone seven years continuous maize cropping from Liuba town located in Zhangye city, Gansu province, PR China. Colonies of strain NC76-1 were white, opaque and circular with a convex shape. The isolate was found to be able to grow at 10-40 °C (optimum 30 °C), pH 6.0 to 12.0 (optimum 7.0-8.0) and with 0-5.0 % (w/v) NaCl (optimum 0%). On the basis of the results of 16S rRNA gene sequence analysis, the strain fell within the clade of the genus , showing the highest sequence similarities with 40 (97.4%), CIP 108388 (97.0%), subsp. TAN 31504 (96.7%) and M1T8B10 (96.7%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between NC76-1 and its closest relatives, 40, CIP 108388, subsp. TAN 31504 and M1T8B10 were ≤73.5 % and 20.3%, respectively. The genomic DNA G+C content of NC76-1 was 61.5 mol%. It presented MK-11 as the predominant menaquinone. The major cellular fatty acids were anteiso-C (49.2 %) and iso-C (35.7%). The major polar lipids were found to be diphosphatidyglycerol, phosphatidylglycerol, phosphatidylethanolamine, aminoglycolipid, five glycolipid and one unidentified lipids. The cell wall amino acids were 2,4-diaminobutyric acid, alanine, glutamic acid, glycine and threonine. On the basis of the phylogenetic, phenotypic and chemotaxonomic characteristics, strain NC76-1 is concluded to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is NC76-1 (GDMCC 1.2286= JCM 34651).
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http://dx.doi.org/10.1099/ijsem.0.005417DOI Listing
August 2022

Prognostic Nutritional Index Predicts Response and Prognosis in Cancer Patients Treated With Immune Checkpoint Inhibitors: A Systematic Review and Meta-Analysis.

Front Nutr 2022 22;9:823087. Epub 2022 Jul 22.

Department of Oncology, Hangzhou Cancer Hospital, Hangzhou, China.

Objective: To investigate the association between pretreatment prognostic nutritional index (PNI) and clinical survival outcomes for advanced-stage cancer patients treated with immune checkpoint inhibitors (ICIs).

Methods: We conducted a comprehensive literature search to identify eligible studies concerning the relationship between pretreatment PNI and survival outcomes in advanced cancer patients treated with ICIs. Published data were extracted and pooled odds ratio (pOR) for objective response rate (ORR), disease control rate (DCR), and pooled hazard ratio (pHR) for overall survival (OS), progressive-free survival (PFS), along with 95% confidence intervals (95% CIs) were calculated.

Results: Twelve studies with 1,359 participants were included in our study. A higher level of PNI indicated a greater ORR (pOR = 2.17, 95% CI = 1.52-3.10) and favorable DCR (pOR = 2.48, 95% CI = 1.87-3.29). Low PNI was associated with a shorter OS (pHR = 2.24, 95% CI = 1.57-3.20) and unfavorable PFS (pHR = 1.61, 95% CI = 1.37-1.88).

Conclusion: Low PNI might be an effective biomarker of poor tumor response and adverse prognosis of advanced cancer patients with ICIs. Further studies are needed to verify the prognostic value of PNI in clinical practice.
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http://dx.doi.org/10.3389/fnut.2022.823087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353139PMC
July 2022

Preparation, purification, and identification of novel antioxidant peptides derived from protein hydrolysates.

Front Nutr 2022 22;9:971419. Epub 2022 Jul 22.

Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.

() protein was hydrolyzed with alkaline protease to obtain antioxidant peptides. The enzymatic hydrolysis conditions were optimized through single-factor and orthogonal experiments. The results showed that the optimal process parameters were using 2% of alkaline protease, and substrate concentration of 1 g/100 mL and hydrolyzed 2 h at pH 8.0. Gel filtration chromatography and RP-HPLC were adopted for isolating and purifying the antioxidant peptides from the protein hydrolysate (GLPH). Three novel antioxidant peptides were identified as LSPGEL (614.68 Da), VYFDR (698.76 Da), and PGPTY (533.57 Da) by nano-HPLC-MS/MS. The results of ABTS free radical scavenging rate demonstrated PGPTY exhibited the best antioxidant activity (IC = 0.24 mg/mL). Moreover, LSPGEL, VYFDR, and PGPTY were docked with Keap1, respectively. The molecular docking results suggested PGPTY had smaller docking energy and inhibition constants than the other two peptides. Finally, the cell viability assay evidenced the protective effect exerted by the antioxidant peptide on HO-induced oxidative damage. Above findings showed the potential of using antioxidant peptides from GLPH as antioxidants.
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http://dx.doi.org/10.3389/fnut.2022.971419DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355161PMC
July 2022

Toward Ending the HIV Epidemic: Temporal Trends and Disparities in Early ART Initiation and Early Viral Suppression Among People Newly Entering HIV Care in the United States, 2012-2018.

Open Forum Infect Dis 2022 Aug 22;9(8):ofac336. Epub 2022 Jul 22.

Division of HIV Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

Background: In 2012, the US Department of Health and Human Services updated their HIV treatment guidelines to recommend antiretroviral therapy (ART) for all people with HIV (PWH) regardless of CD4 count. We investigated recent trends and disparities in early receipt of ART prescription and subsequent viral suppression (VS).

Methods: We examined data from ART-naïve PWH newly presenting to HIV care at 13 North American AIDS Cohort Collaboration on Research and Design clinical cohorts in the United States during 2012-2018. We calculated the cumulative incidence of early ART (within 30 days of entry into care) and early VS (within 6 months of ART initiation) using the Kaplan-Meier survival function. Discrete time-to-event models were fit to estimate unadjusted and adjusted associations of early ART and VS with sociodemographic and clinical factors.

Results: Among 11 853 eligible ART-naïve PWH, the cumulative incidence of early ART increased from 42% in 2012 to 82% in 2018. The cumulative incidence of early VS among the 8613 PWH who initiated ART increased from 83% in 2012 to 93% in 2018. In multivariable models, factors independently associated with delayed ART and VS included non-Hispanic/Latino Black race, residence in the South census region, being a male with injection drug use acquisition risk, and history of substance use disorder (SUD; all ≤ .05).

Conclusions: Early ART initiation and VS have substantially improved in the United States since the release of universal treatment guidelines. Disparities by factors related to social determinants of health and SUD demand focused attention on and services for some subpopulations.
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http://dx.doi.org/10.1093/ofid/ofac336DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348610PMC
August 2022

Relationship between the height of fibular head and the incidence and severity of knee osteoarthritis.

Open Med (Wars) 2022 22;17(1):1330-1337. Epub 2022 Jul 22.

Department of Joint Orthopaedic, Baoji Hospital of Traditional Chinese Medicine, Baoji, Shaanxi, 721000, China.

The purpose of this study was to investigate the correlation between fibular head height and the incidence and severity of osteoarthritis associated with varus knee deformity. The fibular head height, joint line convergence angle (JLCA) and medial proximal tibial angle (MPTA) were measured in a three-dimensional model. Ordinal multivariate logistic regression was used to analyze the correlation between fibular head height and Kellgren-Lawrence (K-L) grade. Pearson correlation was used to analyze the correlation between fibular head height and K-L grade. A total of 232 patients (232 knees) were finally included in the study. There were significant differences in JLCA and hip-knee-ankle angle ( < 0.05), and both JLCA and hip-knee-ankle angle increased with severe aggravation of K-L grade. Both fibular head height and MPTA decreased as the K-L grade was severely aggravated. There was a significant negative correlation between K-L grade and fibular head height ( = -0.812, < 0.001). Furthermore, there was a significant negative correlation between fibular head height and hip-knee-ankle angle ( = -0.7905, < 0.001). In addition to body mass index, fibular head height is a risk factor for the pathogenesis of osteoarthritis associated with varus knee deformity; the smaller the fibular head height, the more severe the degree of varus deformity.
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http://dx.doi.org/10.1515/med-2022-0523DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307141PMC
July 2022

Relationship Between Hardiness and Social Anxiety in Chinese Impoverished College Students During the COVID-19 Pandemic: Moderation by Perceived Social Support and Gender.

Front Psychol 2022 20;13:926863. Epub 2022 Jul 20.

Hainan Vocational University of Science and Technology, Haikou, China.

During the COVID-19 epidemic, quarantine and financial disadvantages might exacerbate social anxiety among impoverished college students. Based on the hardiness model and the social support buffering model, the present study proposed and verified a dual moderation model to investigate the effects of hardiness on social anxiety and the moderating roles of gender and perceived social support. The hardiness scale, the perceived social support scale, and the social anxiety subscale of the self-consciousness scale were administered to 673 Chinese college students aged 18 to 23 years who were recognized as impoverished by the Chinese authorities and provided with funding. The results revealed that (1) hardiness had a significant negative effect on social anxiety, (2) perceived social support moderated the effect of hardiness on social anxiety, and (3) gender moderated the effect of hardiness on social anxiety. The dual moderated model proposed in the study provides practical implications for helping impoverished college students cope with social anxiety during the COVID-19 pandemic.
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http://dx.doi.org/10.3389/fpsyg.2022.926863DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347305PMC
July 2022

Efficacy and Safety of Curcumin and Extract in the Treatment of Arthritis: A Systematic Review and Meta-Analysis of Randomized Controlled Trial.

Front Immunol 2022 22;13:891822. Epub 2022 Jul 22.

Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, National Clinical Research Center for Dermatologic and Immunologic Diseases (NCRC-DID), Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, China.

Background: Modern pharmacological research found that the chemical components of are mainly curcumin and turmeric volatile oil. Several recent randomized controlled trials (RCT) have shown that curcumin improves symptoms and inflammation in patients with arthritis.

Methods: Pubmed, Cochran Library, CNKI, and other databases were searched to collect the randomized controlled trials (RCTs). Then, the risk of bias of RCTs were assessed and data of RCTs were extracted. Finally, RevMan 5.3 was utilized for meta-analysis.

Results: Twenty-nine (29) RCTs involving 2396 participants and 5 types of arthritis were included. The arthritis included Ankylosing Spondylitis (AS), Rheumatoid Arthritis (RA), Osteoarthritis (OA), Juvenile idiopathic arthritis (JIA) and gout/hyperuricemia. Curcumin and Curcuma longa Extract were administered in doses ranging from 120 mg to 1500 mg for a duration of 4-36 weeks. In general, Curcumin and Curcuma longa Extract showed safety in all studies and improved the severity of inflammation and pain levels in these arthritis patients. However, more RCTs are needed in the future to elucidate the effect of Curcumin and Curcuma longa Extract supplementation in patients with arthritis, including RA, OA, AS and JIA.

Conclusion: Curcumin and Curcuma longa Extract may improve symptoms and inflammation levels in people with arthritis. However, due to the low quality and small quantity of RCTs, the conclusions need to be interpreted carefully.
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http://dx.doi.org/10.3389/fimmu.2022.891822DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353077PMC
August 2022

Development and validation of a hypoxia-stemness-based prognostic signature in pancreatic adenocarcinoma.

Front Pharmacol 2022 21;13:939542. Epub 2022 Jul 21.

Department of Clinical Laboratory, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Linhai, China.

Pancreatic adenocarcinoma (PAAD) is one of the most aggressive and fatal gastrointestinal malignancies with high morbidity and mortality worldwide. Accumulating evidence has revealed the clinical significance of the interaction between the hypoxic microenvironment and cancer stemness in pancreatic cancer progression and therapies. This study aims to identify a hypoxia-stemness index-related gene signature for risk stratification and prognosis prediction in PAAD. The mRNA expression-based stemness index (mRNAsi) data of PAAD samples from The Cancer Genome Atlas (TCGA) database were calculated based on the one-class logistic regression (OCLR) machine learning algorithm. Univariate Cox regression and LASSO regression analyses were then performed to establish a hypoxia-mRNAsi-related gene signature, and its prognostic performance was verified in both the TCGA-PAAD and GSE62452 corhorts by Kaplan-Meier and receiver operating characteristic (ROC) analyses. Additionally, we further validated the expression levels of signature genes using the TCGA, GTEx and HPA databases as well as qPCR experiments. Moreover, we constructed a prognostic nomogram incorporating the eight-gene signature and traditional clinical factors and analyzed the correlations of the risk score with immune infiltrates and immune checkpoint genes. The mRNAsi values of PAAD samples were significantly higher than those of normal samples ( < 0.001), and PAAD patients with high mRNAsi values exhibited worse overall survival (OS). A novel prognostic risk model was successfully constructed based on the eight-gene signature comprising JMJD6, NDST1, ENO3, LDHA, TES, ANKZF1, CITED, and SIAH2, which could accurately predict the 1-, 3-, and 5-year OS of PAAD patients in both the training and external validation datasets. Additionally, the eight-gene signature could distinguish PAAD samples from normal samples and stratify PAAD patients into low- and high-risk groups with distinct OS. The risk score was closely correlated with immune cell infiltration patterns and immune checkpoint molecules. Moreover, calibration analysis showed the excellent predictive ability of the nomogram incorporating the eight-gene signature and traditional clinical factors. We developed a hypoxia-stemness-related prognostic signature that reliably predicts the OS of PAAD. Our findings may aid in the risk stratification and individual treatment of PAAD patients.
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http://dx.doi.org/10.3389/fphar.2022.939542DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350896PMC
July 2022

Retinal and choroidal microvascular alterations in Behcet's disease without ocular manifestations: A systematic review and meta-analysis.

Front Med (Lausanne) 2022 22;9:911990. Epub 2022 Jul 22.

Department of Ophthalmology, Lishui Municipal Central Hospital, The Fifth Affiliated Hospital of Wenzhou Medical University, Lishui, China.

Purpose: We performed a systematic review and meta-analysis to examine the microvascular alterations in non-ocular Behcet's disease (BD) using optical coherence tomography angiography (OCTA).

Methods: A comprehensive search was performed in Pubmed, Embase and Cochrane databases for eligible studies from inception to February 2022. Detailed clinical demographics were extracted from each study by two independent reviewers. The weighted mean difference (WMD) and 95% confidence intervals (CI) were used to compare the OCTA parameters between non-ocular BD and healthy controls. Stata 12.0 was adopted to conduct statistical analyses.

Results: Ten cross-sectional studies involving 386 eyes in non-ocular BD and 418 eyes in healthy volunteers were ultimately included in the present analysis. When considering superficial capillary plexus (SCP) and deep capillary plexus (DCP), no significant differences of vessel densities in the whole enface image, fovea and perifovea were evaluated between two groups. Significantly reduced parafoveal vessel density of SCP was observed in non-ocular BD in comparison with healthy group (WMD = -1.33, 95%CI: -1.78, -0.89; = 0.6%), while slightly decreased parafoveal vessel density was assessed in DCP (WMD = -1.47, 95%CI: -3.30, 0.35; = 89.3%). Significantly increasing foveal avascular zone (FAZ) area was observed in non-ocular BD when compared to healthy controls (WMD = 0.11, 95%CI: 0.03, 0.19; = 95.3%). There was no significant difference in flow area of choriocapillaris between non-ocular BD and control group (WMD = 0.06, 95%CI: -0.19, 0.32; = 0%).

Conclusion: Based on current analysis, our results demonstrated significantly lower parafoveal vessel density of SCP and lager FAZ area in full vasculature in non-ocular BD. The retinal microvascular alterations appear before the emergence of ocular manifestations.

Systematic Trial Registration: [https://www.crd.york.ac.uk/PROSPERO/], identifier [CRD42021244856].
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http://dx.doi.org/10.3389/fmed.2022.911990DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9353174PMC
July 2022

Cis- and trans-binding influences in [NUO·(N)].

J Chem Phys 2022 Aug;157(5):054301

Key Laboratory of Organo-Optoelectronics & Molecular Engineering, Department of Chemistry, Tsinghua University, Beijing 100084, China.

Uranium nitride-oxide cations [NUO] and their complexes with equatorial N ligands, [NUO·(N)] (n = 1-7), were synthesized in the gas phase. Mass-selected infrared photodissociation spectroscopy and quantum chemical calculations confirm [NUO·(N)] to be a sterically fully coordinated cation, with electronic singlet ground state of A, linear [NUO] core, and C structure. The presence of short N-U bond distances and high stretching modes, with slightly elongated U-O bond distances and lowered stretching modes, is rationalized by attributing them to cooperative covalent and dative [ǀN≡U≡Oǀ] triple bonds. The mutual trans-interaction through flexible electronic U-5f6d7sp valence shell and the linearly increasing perturbation with increase in the number of equatorial dative N ligands has also been explained, highlighting the bonding characteristics and distinct features of uranium chemistry.
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http://dx.doi.org/10.1063/5.0098068DOI Listing
August 2022

Interactions between polypropylene microplastics (PP-MPs) and humic acid influenced by aging of MPs.

Water Res 2022 Jul 28;222:118921. Epub 2022 Jul 28.

Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou 310014, PR China. Electronic address:

As an emerging pollutant, microplastics (MPs) may interact with dissolved organic matter (DOM) which is prevalent in the aqueous environment. Meanwhile, the aging of MPs in the actual environment increases the uncertainty of their environmental fate. Here, the interaction mechanisms between pristine and aged polypropylene microplastics (PP-MPs) and humic acid (HA) at pH 7.0 were explored. Microstructural changes of HA were examined by fluorescence and Fourier transformation infrared (FT-IR) spectroscopy. Atomic force microscopy coupled with infrared (AFM-IR) and micro-Raman techniques were used to characterize and analyze the interacted PP-MPs. The addition of HA increased the surface roughness of both pristine and aged PP-MPs. Results of AFM-IR and Raman spectra showed that the interaction of PP-MPs with HA accelerated their surface oxidation and enhanced the characteristic signals. XPS spectra showed that the oxygen content ratio of pristine and aged PP-MPs increased by 0.95% and 1.48% after the addition of HA, respectively. PP-MPs after aging interacted more strongly with HA and there was a higher affinity between them. Two-dimensional correlation spectroscopy (2D-COS) combined with FT-IR spectra further elucidated the interaction mechanism at the molecular level. This work will help to evaluate the environmental impact of MPs in ecosystems and understand their interactions with DOM.
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http://dx.doi.org/10.1016/j.watres.2022.118921DOI Listing
July 2022

Growth, physiological, biochemical and molecular changes in plants by magnetic field: A review.

Plant Biol (Stuttg) 2022 Aug 5. Epub 2022 Aug 5.

College of Agronomy, Northwest A&F University, Yangling, 712100, China.

Geomagnetic field (GMF) is an inescapable environmental factor for plants on earth that affects all the growth and yield parameters, but both strong and weak magnetic fields (MF) as compared to GMF can cause a specific role in plant growth and development. MF technology is an eco-friendly technique that does not emit any kind of waste nor generate harmful radiation, and neither requires any external power supply, so it can be used for sustainable and desirable modern agriculture. Thus, the exposure of plants to MF is one of the potential, affordable, reusable, and safe physical practices for enhancing crop productivity by changing physiological and biochemical processes. However, the effect of MF on plant physiological and biochemical processes is not well understood to date. This review describes the effect of altering MF conditions either higher or lower values than GMF on the physiological and biochemical processes of plants. The contradictory and inconsistency present in outcomes from the studies elucidate varying effects of MF on plants that may be due to species dependence and/ or reliance on MF exposure time and intensity. All the outcomes of reviewed literature come to the point that MF has a putative role in changing the physiological processes such as respiration, photosynthesis, nutrient uptake, water relations, and biochemical attributes that includes reactive oxygen species, antioxidants, enzymes, proteins, and secondary metabolites at the genetic level. Moreover, this study suggest MF application efficiently increases the growth and yield attributes of many crops, so recommended for future researches.
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http://dx.doi.org/10.1111/plb.13459DOI Listing
August 2022

Stable and large-scale organic-inorganic halide perovskite nanocrystal/polymer nanofiber films prepared a green fiber spinning chemistry method.

Nanoscale 2022 Aug 5. Epub 2022 Aug 5.

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Jiangsu Key Laboratory of Fine Chemicals and Functional Polymer Materials, Nanjing Tech University, No. 5 Xin Mofan Road, Nanjing 210009, P. R. China.

Organic-inorganic halide perovskite nanocrystals (PNCs) have shown great advantages in recent years due to their tunable emission wavelengths, narrow full-width at half-maximum (FWHM) and high photoluminescence quantum yield (PLQY). However, PNCs still face the challenges of poor stability, difficulty in processing and generation of heavy metal wastes; therefore, it is necessary to develop a green synthetic method to prepare PNCs. Here, we present for the first time a facile fiber spinning chemistry (FSC) method for the rapid preparation of organic-inorganic halide PAN/MAPbX (MA = CHNH, X = Cl, Br and I) nanofiber films at room temperature. The FSC process utilizes spinning fibers as the reactor, and polymer solidification and the generation of PNCs occur simultaneously with solvent evaporation during the spinning process. This method not only achieves a continuous large-scale preparation of PNC/polymer nanofiber films but also avoids the generation of heavy metal waste. The organic-inorganic halide PAN/MAPbX nanofiber films fabricated by FSC demonstrated tunable emission in the range of 464-612 nm and PLQY of up to 58%, and the fluorescence intensity remained essentially unchanged after 90 days of storage in the atmospheric environment. Interestingly, we successfully prepared high-efficiency white light-emitting diodes (WLEDs) and wide color gamut liquid crystal displays (LCDs) with a color gamut of 116.1% using PAN/MAPbBr nanofiber films as fluorescence conversion materials. This study provides a novel way to construct high-performance PNC/polymer fiber composites on a large scale.
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http://dx.doi.org/10.1039/d2nr01691eDOI Listing
August 2022

Influence of root distribution patterns on soil dynamic characteristics.

Sci Rep 2022 Aug 4;12(1):13448. Epub 2022 Aug 4.

National Institute of Natural Hazards, Ministry of Emergency Management of China, Beijing, 100083, People's Republic of China.

Slopes along the highway and railway routes are subjected to not only static loads but also dynamic loads generated by vehicles and trains. The induced excessive deformation potentially poses a threat to slope stability. In terms of the extensive application of ecological slope protection, plants play a critical role in slope stability, as the roots can enhance the shear strength of the soil. This study aims to investigate the influence of different root distribution patterns on the dynamic characteristics induced by cyclic loading. By conducting a group of dynamic triaxial tests, the results indicate that the root system can significantly enhance the liquefaction resistance of the soil when the soil is subjected to lower dynamic loads, and the cross arrangement has a better-reinforced effect than the mixed arrangement. The reinforced effect was not obvious when the soil was subjected to a dynamic load with a larger stress amplitude. In addition, based on the validation of the seed model, a new pore water pressure development model was proposed according to the test results. Overall, the research provides a new model and some innovative observations to better understand the dynamic behavior of root-reinforced soil.
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http://dx.doi.org/10.1038/s41598-022-17828-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352746PMC
August 2022

Resveratrol suppressed microglia activation and promoted functional recovery of traumatic spinal cord via improving intestinal microbiota.

Pharmacol Res 2022 Aug 1:106377. Epub 2022 Aug 1.

Department of Basic Medicine Sciences, and Department of Orthopaedics of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China. Electronic address:

Spinal cord injury (SCI) can change the intestinal microbiota pattern and modify corresponding metabolites, which in turn affect the prognosis of SCI. Among many metabolites, short-chain fatty acids (SCFAs) are critical for neurological recovery after SCI. Recent research showed that resveratrol exerts anti-inflammatory properties. But it is unknown if the anti-inflammatory properties of resveratrol are associated with intestinal microbiota and metabolites. Here, we thus investigated the alteration in gut microbiota and determined the consequent change of SCFAs following resveratrol treatment. The SCI mouse models with retention of gut microbiota (donor) and depletion of gut microbiota (recipient) were established. Fecal microbiota transplantation from donors to recipients was performed with intragastrical administration. Spinal cord tissues of mice were examined by H&E, Nissl, and immunofluorescence staining. The expressions of the inflammatory profile were examined by qPCR and cytometric bead array. Fecal samples of mice were collected and sent to 16S rRNA sequencing. The results showed that resveratrol inhibited the microglial activation and promoted the functional recovery of SCI. The analysis of intestinal microbiota and metabolite indicated that SCI caused dysbiosis and the decrease in butyrate, while resveratrol restored microbiota pattern, reversed intestinal dysbiosis, and increased the concentration of butyrate. Both fecal supernatants from resveratrol-treated donors and separate butyrate suppressed the expression of proinflammation genes in BV2 microglia. Our result demonstrated that fecal microbiota transplantation from resveratrol-treated donors had beneficial effects on the functional recovery of SCI. One mechanism of resveratrol effects was to restore the disrupted gut microbiota and butyrate to normal patterns.
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http://dx.doi.org/10.1016/j.phrs.2022.106377DOI Listing
August 2022

Non-agricultural source dominates the ammonium aerosol in the largest city of South China based on the vertical δN measurements.

Sci Total Environ 2022 Aug 1;848:157750. Epub 2022 Aug 1.

State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, China; CAS Center for Excellence in Deep Earth Science, Guangzhou, China.

Ammonia (NH) is the most prevalent alkaline gas in the atmosphere and plays a critical role in air pollution and public health. However, scientific debate remains over whether agricultural emissions (e.g., livestock and fertilizer application) dominate NH in urban atmosphere in China, which is one of the largest NH emitters in the world. In this study, we first simultaneously collected the fine atmospheric particles (PM) at two heights (ground and 488 m) using the atmospheric observatories in Canton Tower, Guangzhou city, China for the measurements of stable nitrogen isotope composition in ammonium (δN-NH). Our results showed that the average δN-NH value at the ground and the 488 m observatory were 16.9 ‰ and 3.8 ‰, respectively, implying that NH aerosols between the two heights probably have different sources. Moreover, we found that the δN-NH value would sharply decrease to -16.7 ‰ when the air masses came from western Guangzhou, where the urbanization is limited compared to other surrounding areas. The Bayesian mixing model indicated that NH aerosol at the ground observatory was mainly derived from non-agricultural activities (76 %, e.g., vehicular exhaust), with the rest from agricultural sources (24 %). As for the 488 m observatory, the contribution of non-agricultural sources was 53 %, which is lower than the ground observatory. This is expected as the lower air receives more impacts from the local urban emission. However, the current "bottom-up" emission inventory illustrates that only ~20 % NH in Guangzhou is associated with non-agricultural emissions, which is significantly lower than our δN-based results. Overall, our findings strongly imply that non-agricultural sources dominate the urban NH in Guangzhou or maybe in adjacent cities of the Pearl River Delta region as well, suggesting that the emission inventory of NH in this region probably is urgently needed to be revisited in future studies.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157750DOI Listing
August 2022

Anti-mesothelin CAR-T immunotherapy in patients with ovarian cancer.

Cancer Immunol Immunother 2022 Aug 4. Epub 2022 Aug 4.

Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China.

Recently, chimeric antigen receptor T cell (CAR-T) therapy has received increasing attention as an adoptive cellular immunotherapy that targets tumors. However, numerous challenges remain for the effective use of CAR-T to treat solid tumors, including ovarian cancer, which is an aggressive and metastatic cancer with a poor therapeutic response. We screened for an effective anti-MSLN single-chain Fv antibody with comparable binding activity and non-off-target properties using human phage display library. A second-generation of anti-MSLN CAR was designed and generated. We demonstrated the efficacy of our anti-MSLN CAR-T cells for ovarian cancer treatment in an in vitro experiment to kill ovarian tumor cell lines. The anti-MSLN CAR-T cells impeded MSLN-positive tumor growth concomitant with a significant increase in cytokine levels compared with the control. Then, we demonstrated the efficacy of anti-MSLN CAR-T cells in an in vivo experiment against ovarian cancer cell-derived xenografts. Furthermore, we herein report three cases with ovarian cancer who were treated with autologous anti-MSLN CAR-T cells and evaluate the safety and effectiveness of adoptive cell therapy. In this investigator-initiated clinical trials, no patients experienced cytokine release syndrome or neurological symptoms over 2 grads. Disease stabilized in two patients, with progression-free survival times of 5.8 and 4.6 months. Transient CAR expression was detected in patient blood after infusion each time. The tumor partially subsided, and the patient's condition was relieved. In conclusion, this work proves the efficacy of the anti-MSLN CAR-T treatment strategy in ovarian cancer and provides preliminary data for the development of further clinical trials.
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http://dx.doi.org/10.1007/s00262-022-03238-wDOI Listing
August 2022

Genome-wide identification, characterization and expression analysis of the BMP family associated with beak-like teeth in .

Front Genet 2022 18;13:938473. Epub 2022 Jul 18.

CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.

Bone morphogenetic proteins (BMPs), which belong to the transforming growth factor beta (TGF-β) family, are critical for the control of developmental processes such as dorsal-ventral axis formation, somite and tooth formation, skeletal development, and limb formation. Despite having typical healing beak-like teeth and tooth development showing a trend from discrete to healing, the potential role of BMPs in the development of the beak-like teeth is incompletely understood. In the present study, 19 and 16 BMP genes were found in and , respectively, and divided into the BMP2/4/16, BMP5/6/7/8, BMP9/10, BMP12/13/14, BMP3/15 and BMP11 subfamilies. Similar TGFb and TGF_β gene domains and conserved protein motifs were found in the same subfamily; furthermore, two common tandem repeat genes (BMP9 and BMP3a-1) were identified in both and . Selection pressure analysis revealed 13 amino acid sites in the transmembrane region of BMP3, BMP7, and BMP9 proteins of and , which may be related to the diversity and functional differentiation of genes within the BMP family. The qPCR-based developmental/temporal expression patterns of BMPs showed a trend of high expression at 30 days past hatching (dph), which exactly corresponds to the ossification period of the bones and beak-like teeth in . Tissue-specific expression was found for the BMP4 gene, which was upregulated in the epithelial and mesenchymal tissues of the beak-like teeth, suggesting that it also plays a regulatory role in the development of the beak-like teeth in . Our investigation not only provides a scientific basis for comprehensively understanding the BMP gene family but also helps screen the key genes responsible for beak-like tooth healing in and sheds light on the developmental regulatory mechanism.
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http://dx.doi.org/10.3389/fgene.2022.938473DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9342863PMC
July 2022

Evaluating environmental quality in Rujigou coalfield, China, using analytic hierarchy process.

Environ Sci Pollut Res Int 2022 Aug 3. Epub 2022 Aug 3.

Key Laboratory of Efficient Utilization of Low and Medium Grade Energy, Ministry of Education, Tianjin University, Tianjin, 300350, China.

Coal is the major fossil fuel used for power generation. Coal mining activities lead to environmental changes to a large extent, such as degradation in the quality of air, water, and soil, changes in landform, land use/land cover, and vegetation distribution. Evaluating the environmental quality is therefore essential to study the nature and impact of mining activities on the environment. The present study attempts to use the analytic hierarchy process (AHP) to assess the environmental quality of Rujigou coalfield that lacked previous such analysis. The criteria used for evaluation were selected through a literature review and extensive field survey. A photo tour of the study area shows the current ground conditions. Weights were assigned to these criteria based on expert opinions, recommendations from published literature, and field investigation. The results indicate that mining activities in the study region most significantly impact the air quality, followed by soil, water, landform, and vegetation. The knowledge of environmental quality can forewarn policymakers and mine managers about impending environmental problems and improve their ability to manage and resolve them. Moreover, the systematic methodological process described in this research can be applied to any study area with similar features to the one investigated in this paper.
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http://dx.doi.org/10.1007/s11356-022-22340-1DOI Listing
August 2022

Intracavity generation of glioma stem cell-specific CAR macrophages primes locoregional immunity for postoperative glioblastoma therapy.

Sci Transl Med 2022 Aug 3;14(656):eabn1128. Epub 2022 Aug 3.

Key Laboratory of Chemical Biology (Ministry of Education), Department of Pharmaceutics, School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, 44 Cultural West Road, Jinan, Shandong Province 250012, China.

Glioblastoma multiforme (GBM) remains incurable despite aggressive implementation of multimodal treatments after surgical debulking. Almost all patients with GBM relapse within a narrow margin around the initial resected lesion due to postsurgery residual glioma stem cells (GSCs). Tracking and eradicating postsurgery residual GSCs is critical for preventing postoperative relapse of this devastating disease, yet effective strategies remain elusive. Here, we report a cavity-injectable nanoporter-hydrogel superstructure that creates GSC-specific chimeric antigen receptor (CAR) macrophages/microglia (MΦs) surrounding the cavity to prevent GBM relapse. Specifically, we demonstrate that the CAR gene-laden nanoporter in the hydrogel can introduce GSC-targeted CAR genes into MΦ nuclei after intracavity delivery to generate CAR-MΦs in mouse models of GBM. These CAR-MΦs were able to seek and engulf GSCs and clear residual GSCs by stimulating an adaptive antitumor immune response in the tumor microenvironment and prevented postoperative glioma relapse by inducing long-term antitumor immunity in mice. In an orthotopic patient-derived glioblastoma humanized mouse model, the combined treatment with nanoporter-hydrogel superstructure and CD47 antibody increased the frequency of positive immune responding cells and suppressed the negative immune regulating cells, conferring a robust tumoricidal immunity surrounding the postsurgical cavity and inhibiting postoperative glioblastoma relapse. Therefore, our work establishes a locoregional treatment strategy for priming cancer stem cell-specific tumoricidal immunity with broad application in patients suffering from recurrent malignancies.
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http://dx.doi.org/10.1126/scitranslmed.abn1128DOI Listing
August 2022

Short-term effect of 0.01% atropine sulphate eye gel on myopia progression in children.

Int J Ophthalmol 2022 18;15(7):1122-1127. Epub 2022 Jul 18.

Department of Pharmacology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an 710061, Shaanxi Pronvince, China.

Aim: To investigate the effect of 0.01% atropine sulphate eye gel on myopia progression and axial elongation in a 6-month treatment in children.

Methods: Totally 185 children aged 6-12y with binocular myopia of 3.0 D or less in both eyes were enrolled in this prospective cohort study. The atropine group (=125) received one drop of 0.01% atropine sulphate eye gel in each eye before bedtime daily. The control group included 60 matched children without drug intervention during the same period. The spherical equivalent and axial length was recorded at baseline and the sixth month of treatment. The efficacy was evaluated by the change of the spherical equivalent and axial length. Adverse events were also recorded.

Results: The average spherical equivalent and axial length at baseline were not statistically significant between the atropine group (-1.64±0.80 D, 24.13±0.76 mm) and the control group (-1.59±0.94 D, 24.06±0.77 mm, >0.05). After 6mo, there was significantly difference in the spherical equivalent progression between the atropine and the control group (-0.27±0.33 -0.60±0.35 D, <0.001), with a relative reduction of 55.0% in myopia progression. The increase in axial elongation in the atropine group was significantly less than control group (0.19±0.14 0.26±0.14 mm, <0.001), with a relative reduction of 26.9% in axial length. The 84.4% and 38.4% of the eyes progressed by less than 0.50 D and remained stable in the atropine group, compared with 51.7% and 4.2% in the control group. No adverse events were observed.

Conclusion: Atropine sulphate eye gel 0.01% can slow down myopia progression and axial elongation in children with a 6-month treatment.
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http://dx.doi.org/10.18240/ijo.2022.07.12DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9318099PMC
July 2022

PPAR-γ alleviates the inflammatory response in TNF-α-induced fibroblast-like synoviocytes by binding to p53 in rheumatoid arthritis.

Acta Pharmacol Sin 2022 Aug 2. Epub 2022 Aug 2.

Inflammation and Immune Mediated Disease Laboratory of Anhui Province, Anhui Institute of Innovative Drugs, School of Pharmacy, Anhui Medical University, Hefei, 230032, China.

Rheumatoid arthritis (RA) is characterized by synovial inflammation, synoviocyte expansion and damage to cartilage and bone. We recently reported that peroxisome proliferator-activated receptor (PPAR)-γ inhibited the proliferation and activation of fibroblast-like synoviocytes (FLS), and was downregulated in RA synovial. In this study we investigated the role of PPAR-γ in RA and the underlying mechanisms. Adjuvant-induced arthritis (AIA) was induced in rats; from D15, AIA rats were orally administered pioglitazone (30 mg·kg·d) or rosiglitazone (4 mg·kg·d) for 14 days. Collagen-induced arthritis (CIA) was induced in wild-type and Ppar-γ mice. We showed that the expression of PPAR-γ was significantly reduced, whereas that of TNF-α was markedly increased in human RA FLS. In CIA mice, knockdown of PPAR-γ expression (Ppar-γ) aggravated the ankle inflammation. Similarly, T0070907 (a PPAR-γ antagonist) or si-PPAR-γ promoted the activation and inflammation of TNF-α-induced FLS in vitro. On the contrary, administration of PPAR-γ agonist pioglitazone or rosiglitazone, or injection of ad-Ppar-γ into the ankle of AIA rat in vivo induced overexpression of PPAR-γ, reduced the paw swelling and inflammation, and downregulated activation and inflammation of FLS in RA. Interesting, injection of ad-Ppar-γ into the ankle also reversed the ankle inflammation in Ppar-γ CIA mice. We conducted RNA-sequencing and KEGG pathway analysis, and revealed that PPAR-γ overexpression was closely related to p53 signaling pathway in TNF-α-induced FLS. Co-IP study confirmed that p53 protein was bound to PPAR-γ in RA FLS. Taken together, PPAR-γ alleviates the inflammatory response of TNF-α-induced FLS by binding p53 in RA.
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http://dx.doi.org/10.1038/s41401-022-00957-9DOI Listing
August 2022

Umbilical cord mesenchymal stem cell-derived exosomes alleviate collagen-induced arthritis by balancing the population of Th17 and regulatory T cells.

FEBS Lett 2022 Aug 2. Epub 2022 Aug 2.

State Key Laboratory of Membrane Biology, School of Medicine, Institute of Precision Medicine, Center for Synthetic and Systems Biology, Tsinghua University, Beijing, 100084, China.

Exosomes released by mesenchymal stem cells (MSCs) are thought to function as extensions of the MSCs. However, it remains unclear whether exosomes derived from human umbilical cord MSCs (HUMSCs) possess immunoregulatory functions in rheumatoid arthritis. We report that when mice with collagen-induced arthritis were injected with exosomes derived from HUMSC (HUMSC-Exo) their paws became less swollen, and they had lower serum pro-inflammatory cytokine and anti-collagen IgG levels, and decreased synovial hyperplasia. The HUMSC-Exo appeared to restore the balance between Th17 and Treg cells, and this effect was accompanied by reduced IL-17 and enhanced TGF-β and IL-10 levels. These findings suggest that HUMSC-Exo function as important regulators of the balance between Th1/Th17 and Treg cells during immune and inflammatory responses.
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http://dx.doi.org/10.1002/1873-3468.14460DOI Listing
August 2022
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