Publications by authors named "Jun Li"

8,872 Publications

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MiR-137 inhibits the proliferation, invasion and migration of glioma via targeting to regulate EZH2.

Genes Genomics 2021 Jun 23. Epub 2021 Jun 23.

The Fourth Department of Neurosurgery, Tangshan Gongren Hospital, No. 27, Wenhua Road, North District, Tangshan, 063000, Hebei, People's Republic of China.

Background: Gliomas are common malignant tumors in the nervous system, known for poor prognosis and low survival rate.

Objective: This study aims to explore functions of miR-137 in glioma progression and identify messenger RNAs (mRNA) regulated by miR-137, which provides new ideas for further exploration of glioma therapeutic targets.

Methods: Gene expression data were downloaded from the Cancer Genome Atlas database, and abnormally expressed miRNAs and mRNAs in glioma were analyzed. The expression of genes in 20 pairs of clinical tissue samples and glioma cell lines were detected through qRT-PCR, and the expression of proteins was detected through Western blot. Changes in cell proliferative level after transfection were detected via CCK8 assay, and changes in cell migratory and invasive abilities were detected by Transwell assay. Besides, dual-luciferase reporter assay was employed to testify binding relationship between two genes.

Results: Our study found that miR-137 was significantly and lowly expressed in glioma tissue and cell lines, and the prognoses of glioma patients with highly expressed miR-137 were more optimistic. Overexpressed miR-137 could remarkably inhibit proliferative, invasive and migratory abilities of glioma cells U87, while transfection of miR-137 inhibitor presented an opposite effect. Additionally, EZH2 was a direct target of miR-137 and overexpressed EZH2 effectively reversed the effect of miR-137 on glioma proliferation and migration.

Conclusions: Our study found that miR-137 could suppress the proliferation, invasion and migration of glioma cells through regulating the expression of EZH2. So far, we have found a novel regulatory pair that influences glioma progression, providing a basis for further development of new therapeutic strategies.
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http://dx.doi.org/10.1007/s13258-021-01117-9DOI Listing
June 2021

HIV viral exposure and mortality in a multicenter ambulatory HIV adult cohort, United States, 1995-2016.

Medicine (Baltimore) 2021 Jun;100(25):e26285

Division of HIV/AIDS Prevention, Centers for Disease Control and Prevention, Atlanta, GA.

Abstract: The aim of this study was to identify viral exposure (VE) measures and their relationship to mortality risk among persons with HIV.Prospective multicenter observational study to compare VE formulae.Eligible participants initiated first combination antiretroviral therapy (cART) between March 1, 1995 and June 30, 2015. We included 1645 participants followed for ≥6 months after starting first cART, with cART prescribed ≥75% of time, who underwent ≥2 plasma viral load (VL) and ≥1 CD4+ T-lymphocyte cell (CD4) measurement during observation. We evaluated all-cause mortality from 6 months after cART initiation until June 30, 2016. VE was quantified using 2 time-updated variables: viremia copy-years and percent of person-years (%PY) spent >200 or 50 copies/mL. Cox models were fit to estimate associations between VE and mortality.Participants contributed 10,453 person years [py], with median 14 VLs per patient. Median %PY >200 or >50 were 10% (interquartile range: 1%-47%) and 26% (interquartile range: 6%-72%), respectively. There were 115 deaths, for an overall mortality rate of 1.19 per 100 person years. In univariate models, each measure of VE was significantly associated with mortality risk, as were older age, public insurance, injection drug use HIV risk history, and lower pre-cART CD4. Based on model fit, most recent viral load and %PY >200 copies/mL provided the best combination of VE factors to predict mortality, although all VE combinations evaluated performed well.The combination of most recent VL and %PY >200 copies/mL best predicted mortality, although all evaluated VE measures performed well.
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http://dx.doi.org/10.1097/MD.0000000000026285DOI Listing
June 2021

Strategies for inclusion of growth factors into 3D printed bone grafts.

Essays Biochem 2021 Jun 22. Epub 2021 Jun 22.

Department of Orthopaedic Surgery, University of Otago Christchurch, Christchurch 8011, New Zealand.

There remains a critical need to develop new technologies and materials that can meet the demands of treating large bone defects. The advancement of 3-dimensional (3D) printing technologies has allowed the creation of personalized and customized bone grafts, with specific control in both macro- and micro-architecture, and desired mechanical properties. Nevertheless, the biomaterials used for the production of these bone grafts often possess poor biological properties. The incorporation of growth factors (GFs), which are the natural orchestrators of the physiological healing process, into 3D printed bone grafts, represents a promising strategy to achieve the bioactivity required to enhance bone regeneration. In this review, the possible strategies used to incorporate GFs to 3D printed constructs are presented with a specific focus on bone regeneration. In particular, the strengths and limitations of different methods, such as physical and chemical cross-linking, which are currently used to incorporate GFs to the engineered constructs are critically reviewed. Different strategies used to present one or more GFs to achieve simultaneous angiogenesis and vasculogenesis for enhanced bone regeneration are also covered in this review. In addition, the possibility of combining several manufacturing approaches to fabricate hybrid constructs, which better mimic the complexity of biological niches, is presented. Finally, the clinical relevance of these approaches and the future steps that should be taken are discussed.
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http://dx.doi.org/10.1042/EBC20200130DOI Listing
June 2021

Deep learning wavefront sensing method for Shack-Hartmann sensors with sparse sub-apertures.

Opt Express 2021 May;29(11):17669-17682

In this letter, we proposed a deep learning wavefront sensing approach for the Shack-Hartmann sensors (SHWFS) to predict the wavefront from sub-aperture images without centroid calculation directly. This method can accurately reconstruct high spatial frequency wavefronts with fewer sub-apertures, breaking the limitation of d/r ≈ 1 (d is the diameter of sub-apertures and r is the atmospheric coherent length) when using SHWFS to detect atmospheric turbulence. Also, we used transfer learning to accelerate the training process, reducing training time by 98.4% compared to deep learning-based methods. Numerical simulations were employed to validate our approach, and the mean residual wavefront root-mean-square (RMS) is 0.08λ. The proposed method provides a new direction to detect atmospheric turbulence using SHWFS.
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http://dx.doi.org/10.1364/OE.427261DOI Listing
May 2021

Cyclodextrin polymer-valved MoS-embedded mesoporous silica nanopesticides toward hierarchical targets via multidimensional stimuli of biological and natural environments.

J Hazard Mater 2021 Jun 13;419:126404. Epub 2021 Jun 13.

Key Laboratory of Mesoscopic Chemistry (Ministry of Education), State Key Laboratory of Coordination Chemistry, and School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, People's Republic of China. Electronic address:

Targeted delivery of pesticides towards pests and pathogens can significantly improve the bioavailability and efficacy of pesticides and minimize the impact on the environment. Cyclodextrin polymer (CDP)-valved, benzimidazole functionalized, MoS-embedded mesoporous silica ([email protected]@CDP) nanopesticides were constructed toward hierarchical biological targets of pests, pathogens, and foliage. The splash and bounce of the aqueous droplets containing [email protected]@CDP nanoparticles in the presence of Aersosol OT on superhydrophobic surfaces were well inhibited available for excellent wetting to prevent pesticides from losing to the environment. The multivalent supramolecular nanovalves between CDP and the functionalized benzimidazole moieties could be activated for the controlled release of pesticides in the cases of low pH and α-amylase. It is the first time to report the foliage-triggered controlled release of pesticides, owing to the competitive binding of epicuticular wax components to CDP. Furthermore, thermogenic MoS cores triggered the controlled release of pesticides under irradiation of near infrared light. The fungicidal efficacies of the stimuli-responsive nanopesticides against pathogenic fungi Rhizoctonia solani and Fusarium graminearum were demonstrated. It is clear that the smart nanopesticides could realize the controlled release of pesticides toward hierarchical biological targets for enhanced pesticide bioavailability and efficacy via the multidimensional stimuli of pH, α-amylase, epicuticular waxes, and sunlight.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126404DOI Listing
June 2021

Enhanced ultra-wide NIR fluorescence in tellurite glass doped with Er-Tm-Nd-Ag NPs.

Spectrochim Acta A Mol Biomol Spectrosc 2021 Jun 11;261:120075. Epub 2021 Jun 11.

College of Information Science and Engineering, Ningbo University, Zhejiang 315211, China. Electronic address:

Tellurite glasses with combination of Er/Tm/Nd ions and silver nanoparticles (Ag NPs) were prepared by using single-step melt-quenching technology, and the enhanced effect of Ag NPs on the ultra-broadband near-infrared (NIR) fluorescence was studied. Under the 808 nm LD excitation, two ultra-broadband NIR fluorescence of 1300-1600 nm and 1600-2100 nm underwent an obvious enhancement of about 52% compared to the tri-doping tellurite glass free of Ag NPs. The intensified local electric field induced by Ag NPs together with the energy transfer from Ag species to doped ions is responsible for this enhancement. The enhanced ultra-broadband NIR fluorescence of 1300-1600 nm with the full width at half maximum (FWHM) of 230 nm, originating from the spectral overlapping of 1.34 µm (F→I of Nd), 1.47 µm (H→F of Tm) and 1.53 µm (I→I of Er) three bands, is promising in developing new ultra-broadband photonic devices such as fiber amplifiers and tunable lasers.
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http://dx.doi.org/10.1016/j.saa.2021.120075DOI Listing
June 2021

Electronic Structure and Spectroscopic Properties of Group-7 Tri-Oxo-Halides MOX (M = Mn-Bh, X = F-Ts).

Inorg Chem 2021 Jun 21. Epub 2021 Jun 21.

Department of Chemistry, Southern University of Science and Technology, Shenzhen 518055, China.

The 24 trioxide halide molecules MOX of the manganese group (M = Mn-Bh; X = F-Ts), which are iso-valence-electronic with the famous MnO ion, have been quantum-chemically investigated by quasi-relativistic density-functional and ab initio correlated approaches. Geometric and electronic structures, valence and oxidation numbers, vibrational and electronic spectral properties, energetic stabilities of the monomers in the gas phase, and the decay mode of MnOF have been investigated. The light Mn-3d species are most strongly electron-correlated, indicating that the concept of a closed-shell Lewis-type single-configurational structure [Mn(d) O(p) F(p)] reaches its limits. The concept of real-valued spin orbitals φ(r)·α and φ(r)·β breaks down for the heavy Bh-6d, At-6p and Ts-7p elements because of the dominating spin-orbit coupling. The vigorous decomposition of MnOF at ambient conditions starts by the autocatalyzed release of O and the formation of MnOF clusters, triggered by the electron-depleted "oxylic" character of the oxide ligands in MnOX.
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http://dx.doi.org/10.1021/acs.inorgchem.1c00626DOI Listing
June 2021

The influential factors and intervention strategies that engage malignant cancer patients in health-promoting behaviors during PICC line maintenance.

Am J Transl Res 2021 15;13(5):5208-5215. Epub 2021 May 15.

Department of Breast and Thoracic Tumors Surgery, The First Affiliated Hospital of Hainan Medical University Haikou 570100, Hainan Province, China.

Objective: To analyze the influential factors and intervention strategies involved in engaging health-promoting behaviors (EHPD) during peripheral central venous catheter (PICC) line maintenance in malignant tumor patients.

Methods: 120 patients with malignant tumors who underwent PICC line maintenance in our hospital were prospectively analyzed. They were divided into a low and moderate level group (HPLP-II score ≤137) and a high level group (HPLP-II score >137) according to their Health Promoting Lifestyle Profile II (HPLP-II) questionnaire scores. Single-factor and multifactor analyses were performed to identify the factors influencing the patients' engagement in self-health-promoting behaviors. The one hundred and twenty patients with malignant tumors were randomly divided into two groups (n=60 in each group). The control group and the intervention group underwent routine nursing care and patient education. The two groups were compared in terms of the changes in their HPLP-II scores, their Cancer Patients PICC Self-management Scale (CPPSM) scores, their SAS and their SDS scores before and after the intervention, as well as their maintenance compliance rates, their complication rates during catheter placement, and their lack of PICC maintenance.

Results: Literacy, place of residence, duration of catheter use, self-management abilities of PICCs, social support, and anxiety were risk factors (>1, <0.05). Compared with their pre-intervention scores, the HPLP-II and CPPSM scores were increased in both groups (<0.05), and the SAS and SDS scores were decreased in both groups after the intervention (<0.05), and the intervention group had higher scores than the control group (<0.05). The intervention group exhibited a higher maintenance compliance rate than the control group (<0.05). The incidence of complications and the lack of PICC maintenance in the intervention group were lower than they were in the control group (<0.05).

Conclusion: The influential factors during PICC maintenance for EHPD in malignant cancer patients include literacy, place of residence, duration of PICC use, . Patient education can promote patients' EHPD and self-management abilities, relieve their anxiety and depression, reduce their complications, and improve their compliance.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205709PMC
May 2021

The Development and Validation of the Psychological Needs of Cancer Patients Scale.

Front Psychol 2021 3;12:658989. Epub 2021 Jun 3.

Department of Psychology, Chongqing University Cancer Hospital, Chongqing, China.

In the present research, the Psychological Needs of Cancer Patients Scale (PNCPS) was developed and validated. Based on Group 1 (400 cancer patients), the exploratory factor analysis identified a 23-item scale with six factors: value and esteem (five items, i.e., reconsider the meaning and purpose of life), independence and control (six items, i.e., private space), mental car (three items, i.e., vent negative emotions), disease care (three items, i.e., acquire knowledge about disease), belonging and companionship (three items, i.e., spend more time at home), and security (three items, i.e., living conditions be better). The structure identified with Group 1 was further tested, based on Group 2 (199 cancer patients), for reliability and validity. The results showed that PNCPS has a clear factor structure and good psychometric characteristics. By taking into account the cultural background of Chinese patients, this scale will advance the study of the psychological needs of those with malignant tumors and thus has a certain reference value for other countries.
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http://dx.doi.org/10.3389/fpsyg.2021.658989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209331PMC
June 2021

Classification of Schizophrenia by Combination of Brain Effective and Functional Connectivity.

Front Neurosci 2021 3;15:651439. Epub 2021 Jun 3.

School of Medical Engineering, Xinxiang Medical University, Xinxiang, China.

At present, lots of studies have tried to apply machine learning to different electroencephalography (EEG) measures for diagnosing schizophrenia (SZ) patients. However, most EEG measures previously used are either a univariate measure or a single type of brain connectivity, which may not fully capture the abnormal brain changes of SZ patients. In this paper, event-related potentials were collected from 45 SZ patients and 30 healthy controls (HCs) during a learning task, and then a combination of partial directed coherence (PDC) effective and phase lag index (PLI) functional connectivity were used as features to train a support vector machine classifier with leave-one-out cross-validation for classification of SZ from HCs. Our results indicated that an excellent classification performance (accuracy = 95.16%, specificity = 94.44%, and sensitivity = 96.15%) was obtained when the combination of functional and effective connectivity features was used, and the corresponding optimal feature number was 15, which included 12 PDC and three PLI connectivity features. The selected effective connectivity features were mainly located between the frontal/temporal/central and visual/parietal lobes, and the selected functional connectivity features were mainly located between the frontal/temporal and visual cortexes of the right hemisphere. In addition, most of the selected effective connectivity abnormally enhanced in SZ patients compared with HCs, whereas all the selected functional connectivity features decreased in SZ patients. The above results showed that our proposed method has great potential to become a tool for the auxiliary diagnosis of SZ.
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http://dx.doi.org/10.3389/fnins.2021.651439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209471PMC
June 2021

MiR-433-3p restrains the proliferation, migration and invasion of glioma cells via targeting SMC4.

Brain Res 2021 Jun 17:147563. Epub 2021 Jun 17.

The Fourth Department of Neurosurgery, Tangshan Gongren Hospital, Tangshan, 063000, China. Electronic address:

Objective: Glioma is a common primary malignant brain tumor characterized by high mortality and poor prognosis. The purpose of this study is to explore the molecular mechanism underlying glioma, aiming to provide a new target for the treatment of glioma to improve the prognosis of patients.

Methods: The differentially expressed genes and regulatory axis affecting the prognosis of glioma were identified with bioinformatics analysis, and the expression of miR-433-3p and SMC4 mRNA was detected with qRT-PCR. The expression of SMC4 and epithelial-mesenchymal transition (EMT)-associated proteins were detected with western blot. The targeting relationship between miR-433-3p and SMC4 was verified with dual-luciferase reporter gene assay. The proliferative ability of glioma cells was detected with CCK-8 assay, while the migration and invasion of glioma cells were detected with Transwell assay.

Results: we found that the expression of SMC4 was significantly up-regulated in glioma, showing that SMC4 was an unfavorable factor for prognosis and could promote the progression of cancer cells. Its upstream regulator miR-433-3p was significantly down-regulated in glioma, which inhibited the development of cancer cells. Moreover, miR-433-3p could target to inhibit the expression of SMC4. Rescue assay showed that miR-433-3p could affect the development of glioma by regulating the expression of SMC4.

Conclusion: Our data demonstrate for the first time that SMC4 is a direct target of miR-433-3p, and elucidate the molecular mechanism by which miR-433-3p inhibits the malignant progression of glioma by targeting and down-regulating the expression of SMC4.
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http://dx.doi.org/10.1016/j.brainres.2021.147563DOI Listing
June 2021

Pectin in biomedical and drug delivery applications: A review.

Int J Biol Macromol 2021 Jun 17;185:49-65. Epub 2021 Jun 17.

Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, PR China. Electronic address:

Natural macromolecules have attracted increasing attention due to their biocompatibility, low toxicity, and biodegradability. Pectin is one of the few polysaccharides with biomedical activity, consequently a candidate in biomedical and drug delivery Applications. Rhamnogalacturonan-II, a smaller component in pectin, plays a major role in biomedical activities. The ubiquitous presence of hydroxyl and carboxyl groups in pectin contribute to their hydrophilicity and, hence, to the favorable biocompatibility, low toxicity, and biodegradability. However, pure pectin-based materials present undesirable swelling and corrosion properties. The hydrophilic groups, via coordination, electrophilic addition, esterification, transesterification reactions, can contribute to pectin's physicochemical properties. Here the properties, extraction, and modification of pectin, which are fundamental to biomedical and drug delivery applications, are reviewed. Moreover, the synthesis, properties, and performance of pectin-based hybrid materials, composite materials, and emulsions are elaborated. The comprehensive review presented here can provide valuable information on pectin and its biomedical and drug delivery applications.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.06.088DOI Listing
June 2021

Comparative Efficacy and Safety of Vancomycin, Linezolid, Tedizolid, and Daptomycin in Treating Patients with Suspected or Proven Complicated Skin and Soft Tissue Infections: An Updated Network Meta-Analysis.

Infect Dis Ther 2021 Jun 18. Epub 2021 Jun 18.

School of Clinical Medical Sciences, Southwest Medical University, Luzhou, 646000, Sichuan, China.

Introduction: Skin and soft structure infections (SSTIs) caused by methicillin-resistant Staphylococcus aureus (MRSA) pose serious health risks and cause significant cost burdens, and a conclusive recommendation about antibiotics has not yet been generated. Therefore, we performed this updated network meta-analysis to determine the preferred drug for the treatment of MRSA-caused SSTIs.

Methods: We searched PubMed, Embase, and Cochrane Library to identify any potentially eligible randomized controlled trials (RCTs) investigating the comparative efficacy and safety of any two of vancomycin, linezolid, tedizolid, and daptomycin in MRSA-caused SSTIs. All statistical analyses were conducted with RevMan, ADDIS, and STATA software.

Results: Twenty eligible RCTs involving 7804 patients were included for the final analysis. Direct meta-analysis suggested that linezolid was superior to vancomycin in improving clinical (odds ratio [OR], 1.46; 95% confidence interval [CI], 1.07-1.99; P = 0.02) and microbiological (OR, 1.89; 95% CI, 1.24-2.86; P = 0.003) success, which were all confirmed by network meta-analyses. No statistical differences were identified regarding other comparisons. Meanwhile, there were no significant differences between any two antibiotics related to safety. Moreover, ranking probabilities indicated that linezolid had the highest probability of being ranked best in terms of clinical and microbiological success.

Conclusion: Based on the limited evidence, linezolid may be a preferred antibiotic for the treatment of MRSA-caused SSTIs because it showed superiority in clinical and microbiological success without difference regarding safety.
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http://dx.doi.org/10.1007/s40121-021-00456-0DOI Listing
June 2021

3D Printing Magnetic Actuators for Biomimetic Applications.

ACS Appl Mater Interfaces 2021 Jun 17. Epub 2021 Jun 17.

CAS Key Laboratory of Mechanical Behavior and Design of Materials, Department of Modern Mechanics, CAS Center for Excellence in Complex System Mechanics, University of Science and Technology of China, Hefei 230027, China.

Biomimetic actuators with stimuli-responsiveness, adaptivity, and designability have attracted extensive attention. Recently, soft intelligent actuators based on stimuli-responsive materials have been gradually developed, but it is still challenging to achieve various shape manipulations of actuators through a simple 3D printing technology. In this paper, a 3D printing strategy based on magneto-active materials is developed to manufacture various biomimetic magnetic actuators, in which the new printable magnetic filament is composed of a thermoplastic rubber material and magnetic particles. The continuous shape transformation of magnetic actuators is further demonstrated to imitate the motion characteristic of creatures, including the predation behavior of octopus tentacles, the flying behavior of the butterfly, and the flower blooming behavior of the plant. Furthermore, the magnetic field-induced deformation of the biomimetic structure can be simulated by the finite element method, which can further guide the structural design of the actuators. This work proves that the biomimetic actuator based on soft magneto-active materials has the advantages of programmable integrated structure, rapid prototyping, remote noncontact actuation, and rapid magnetic response. As a result, this 3D printing method possesses broad application prospects in soft robotics and other fields.
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http://dx.doi.org/10.1021/acsami.1c08252DOI Listing
June 2021

An injectable and self-healing hydrogel with controlled release of curcumin to repair spinal cord injury.

Bioact Mater 2021 Dec 28;6(12):4816-4829. Epub 2021 May 28.

MOE Joint International Research Laboratory of CNS Regeneration, Jinan University, Guangzhou, 510632, China.

The harsh local micro-environment following spinal cord injury (SCI) remains a great challenge for neural regeneration. Local reconstitution of a favorable micro-environment by biocompatible scaffolds with desirable functions has thus been an area of concern. Herein, a hybrid hydrogel was developed using Fmoc-grafted chitosan (FC) and Fmoc peptide (FI). Dynamic reversible π-π stacking interactions of the fluorenyl rings enabled the FC/FI hybrid hydrogel to exhibit excellent injectable and self-healing properties, as characterized by visual appearances and rheological tests. Furthermore, the FC/FI hybrid hydrogel showed a slow and persistent release of curcumin (Cur), which was named as FC/FI-Cur hydrogel. studies confirmed that with the support of FC/FI-Cur hydrogel, neurite outgrowth was promoted, and Schwann cell (SC) migration away from dorsal root ganglia (DRG) spheres with enhanced myelination was substantiated. The FC/FI-Cur hydrogel well reassembled extracellular matrix at the lesion site of rat spinal cord and exerted outstanding effects in modulating local inflammatory reaction by regulating the phenotypes of infiltrated inflammatory cells. In addition, endogenous SCs were recruited in the FC/FI-Cur graft and participated in the remyelination process of the regenerated nerves. These outcomes favored functional recovery, as evidenced by improved hind limbs movement and enhanced electrophysiological properties. Thus, our study not only advanced the development of multifunctional hydrogels but also provided insights into comprehensive approaches for SCI repair.
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http://dx.doi.org/10.1016/j.bioactmat.2021.05.022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175285PMC
December 2021

The feasibility and technical strategy of a fascia space priority approach in laparoscopic lateral lymph node dissection for advanced middle and low rectal cancer: a retrospective multicentre study.

Wideochir Inne Tech Maloinwazyjne 2021 Jun 9;16(2):312-320. Epub 2021 Apr 9.

Department of Colorectal Surgery, Tianjin Union Medical Centre, Tianjin, China.

Introduction: Laparoscopic lateral lymph node dissection (LLND) is an important treatment for patients with lateral lymph node metastasis.

Aim: To assess the technical feasibility and investigate the surgical outcomes after LLND using the fascia space priority approach for patients with advanced middle and low rectal cancer.

Material And Methods: Consecutive patients undergoing laparoscopic LLND using the fascia space priority approach from June 2017 to June 2020 were identified from 12 medical centres in mainland China. Three anatomic fascia spaces were dissected to establish the boundaries of the LLND, and the obturator and internal iliac lymph nodes were excised in an en bloc manner. Retrospective clinical data including patient characteristics, surgical details, and pathology were analysed.

Results: A total of 112 patients were identified. All surgeries were completed laparoscopically with no conversions. The mean operation time was 343.6 ±103.8 min for the entire procedure. The median blood loss was 100 ml (range: 100-700 ml). The median lymph node yield was 6 (range: 1-41), and lymph nodes were positive in 39.3% (44/112) of the patients. Sixteen (14.3%) patients had Clavien-Dindo I-II complications, no Clavien-Dindo III-IV complications were identified. The incidence of complications between the bilateral dissection group and the unilateral dissection group was not statistically different (p = 0.19). The complication rate between the "nCRT" group and the "no nCRT" group was not significantly different (p = 0.62) either. There were no perioperative deaths.

Conclusions: Laparoscopic LLND using the fascia space priority approach is feasible and safe for patients with lateral lymph node metastasis.
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http://dx.doi.org/10.5114/wiitm.2021.105143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193747PMC
June 2021

Insight into Organic Pollutant Adsorption Characteristics on a g-CN Surface by Attenuated Total Reflection Spectroscopy and Molecular Dynamics Simulation.

Langmuir 2021 Jun 15. Epub 2021 Jun 15.

State Key Laboratory of Pollution Control and Resource Reuse, Jiangsu Key Laboratory of Vehicle Emissions Control, School of the Environment, Nanjing University, Nanjing 210023, P. R. China.

Herein the adsorption characteristics of zwitterionic dye pollutant Rhodamine B (RhB) on a g-CN surface were investigated by both an attenuated total reflection spectroscopy (ATRS) experiment and a molecular dynamics simulation (MDS). For experimental investigation, g-CN was coated on a silica optical fiber (SOF) surface to fabricate an adsorption film. According to the ATRS response, adsorption thermodynamics and thermodynamics results were in situ obtained and evaluated. The isothermal Langmuir model was used to calculate the adsorption equilibrium constants () and adsorption energies (Δ) for RhB as 27.25 × 10 M and -31.01 kJ mol, respectively, which indicated the spontaneous adsorption behavior of RhB at the g-CN surface. Using dynamic Elovich modeling, the rate constants of RhB were found to be = 0.0063 min and = 0.0004 min, which indicated two-stage adsorption at the g-CN surface. For theoretical simulation, adsorption configurations and adsorption energies were systematically calculated by a molecular dynamics simulation (MDS) . RhB molecules were inclined to orient in a parallel position at the g-CN surface during low concentration but a perpendicular position at the g-CN surface during high concentration. Combined with experimental and calculation results, this work revealed the microscopic adsorption performance and elucidated the intermolecular interaction between localized interfaces of g-CN and hazardous dye pollutant. We propose an adsorption model to explain the process of surface interaction, which is based on molecular orientation and a force-driven mechanism. Electrostatic attraction and π-π interaction dominated the adsorption interaction with an adsorption energy of Δ = -38.96 kJ mol for low RhB concentration, and electrostatic attraction dominated the adsorption interaction with an adsorption energy of Δ = -25.76 kJ mol for high RhB concentration. This work can provide a fundamental basis for a dye-pollutants removal application by g-CN in both adsorption and photocatalyzation.
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http://dx.doi.org/10.1021/acs.langmuir.1c00360DOI Listing
June 2021

Diaphragma sellae sinking can predict the onset of hyponatremia after transsphenoidal surgery for pituitary adenomas.

J Endocrinol Invest 2021 Jun 14. Epub 2021 Jun 14.

Fuzong Clinical Medical College of Fujian Medical University, Fuzhou, 350025, China.

Purpose: Sinking of the diaphragma sellae (DS) may stretch the pituitary stalk, which in turn impairs neurohypophyseal function; thus, it may play a role in the development of postoperative hyponatremia. We aimed to assess the factors influencing the development of hyponatremia after transsphenoidal surgery (TSS) for pituitary adenomas and analyze the effect of DS sinking on hyponatremia.

Methods: After applying the inclusion and exclusion criteria, we retrospectively analyzed the clinical data of patients with pituitary adenoma who underwent TSS. The pituitary gland was scanned using a 3.0-T magnetic resonance imaging, and sagittal and coronal images were acquired. We evaluated the following: preoperative and postoperative hypothalamus‒pituitary‒thyroid axis function, hypothalamus‒pituitary‒adrenal axis function, intra-operative cerebrospinal fluid leaks, diabetes insipidus, hyponatremia, time from the day of surgery to the day of discharge, and time of hyponatremia onset.

Results: Of the 460 patients who had microscopic TSS for pituitary adenoma, 83 experienced postoperative hyponatremia. Hyponatremia occurred approximately 5.25 days postoperatively and persisted for 5.54 days. The lowest average blood sodium level was 123.9 mEq/L, which occurred at 7.49 days after surgery. Logistic regression analysis showed that the risk of hyponatremia was greater for patients with a significant DS sinking depth, a large pituitary stalk deviation angle difference, and a longer postoperative "measurable pituitary stalk". The difference in blood sodium levels between pre-TSS and 2 days post-TSS was also an independent predictor of postoperative hyponatremia onset.

Conclusion: DS sinking plays an important role in predicting hyponatremia onset after TSS for pituitary adenomas.
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http://dx.doi.org/10.1007/s40618-021-01611-7DOI Listing
June 2021

Host and gut microbial tryptophan metabolism and type 2 diabetes: an integrative analysis of host genetics, diet, gut microbiome and circulating metabolites in cohort studies.

Gut 2021 Jun 14. Epub 2021 Jun 14.

Department of Epidemiology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, Maryland, USA.

Objective: Tryptophan can be catabolised to various metabolites through host kynurenine and microbial indole pathways. We aimed to examine relationships of host and microbial tryptophan metabolites with incident type 2 diabetes (T2D), host genetics, diet and gut microbiota.

Method: We analysed associations between circulating levels of 11 tryptophan metabolites and incident T2D in 9180 participants of diverse racial/ethnic backgrounds from five cohorts. We examined host genome-wide variants, dietary intake and gut microbiome associated with these metabolites.

Results: Tryptophan, four kynurenine-pathway metabolites (kynurenine, kynurenate, xanthurenate and quinolinate) and indolelactate were positively associated with T2D risk, while indolepropionate was inversely associated with T2D risk. We identified multiple host genetic variants, dietary factors, gut bacteria and their potential interplay associated with these T2D-relaetd metabolites. Intakes of fibre-rich foods, but not protein/tryptophan-rich foods, were the dietary factors most strongly associated with tryptophan metabolites. The fibre-indolepropionate association was partially explained by indolepropionate-associated gut bacteria, mostly fibre-using . We identified a novel association between a host functional variant (determining lactase persistence) and serum indolepropionate, which might be related to a host gene-diet interaction on gut , a probiotic bacterium significantly associated with indolepropionate independent of other fibre-related bacteria. Higher milk intake was associated with higher levels of gut and serum indolepropionate only among genetically lactase non-persistent individuals.

Conclusion: Higher milk intake among lactase non-persistent individuals, and higher fibre intake were associated with a favourable profile of circulating tryptophan metabolites for T2D, potentially through the host-microbial cross-talk shifting tryptophan metabolism toward gut microbial indolepropionate production.
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http://dx.doi.org/10.1136/gutjnl-2021-324053DOI Listing
June 2021

Fabrication of self-healing pectin/chitosan hybrid hydrogel via Diels-Alder reactions for drug delivery with high swelling property, pH-responsiveness, and cytocompatibility.

Carbohydr Polym 2021 Sep 24;268:118244. Epub 2021 May 24.

College of Chemical Engineering, Xinjiang Agricultural University, Urumchi 830052, Xinjiang, People's Republic of China. Electronic address:

Self-healing hydrogels with pH-responsiveness could protect loaded drugs from being destroyed till it arrives to the target. The pectin-based hydrogel is a candidate due to the health benefit, anti-inflammation, antineoplastic activity, nontoxicity, and biospecific degradation, et al. However, the abundant existence of water-soluble branched heteropolysaccharide chains influenced its performance resulting in limitation of the potential. In the present study, we prepared a series of self-healing pectin/chitosan hydrogels via the Diels-Alder reaction. Moreover, pectin/chitosan composite hydrogel was prepared as a contrast. By comparison, it can be seen that the Diels-Alder reaction greatly improved the cross-linking density of hydrogels. The self-healing experiments showed excellent self-healing performance. In different swelling mediums, significant transformation in the swelling ratio was shown, indicating well-swelling property, pH- and thermo-responsiveness. The drug loading and release studies presented high loading efficiency and sustained release performance. The cytotoxicity assay that showed a high cell proliferation ratio manifested great cytocompatibility.
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http://dx.doi.org/10.1016/j.carbpol.2021.118244DOI Listing
September 2021

Sensitive fluorescence and visual detection of organophosphorus pesticides with a Ru(bpy)-ZIF-90-MnO sensing platform.

Anal Methods 2021 Jun 14. Epub 2021 Jun 14.

Hunan Provincial Key Laboratory of Materials Protection for Electric Power and Transportation, Hunan Provincial Key Laboratory of Cytochemistry, School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha, 410114, P. R. China.

Fluorescence sensing organophosphorus pesticides (OPs) is of great importance for both food safety and global environment; however, the reported fluorescent probes are usually directly exposed to the external environment, resulting in premature leakage or photobleaching and thus limiting their photostability and assay sensitivity. In this work, a fluorescent sensing platform consisting of a novel luminescent metal-organic framework (Ru(bpy)32+-ZIF-90) and manganese dioxide nanosheets (MnO2 NSs) was prepared for sensing OPs. Due to the protection and improvement in the fluorescence of Ru(bpy)32+ by ZIF-90, the Ru(bpy)32+-ZIF-90 probe displayed remarkable photostability and high stability in water. By virtue of the high stability of Ru(bpy)32+-ZIF-90, as well as the outstanding fluorescence quenching and notable recognition ability of the MnO2 NSs, this sensing platform provided excellent detection capability for parathion-methyl, with a wide concentration range of 0.050-60 ng mL-1 and a low detection limit of 0.037 ng mL-1. Additionally, the system exhibited a visual color change with the concentration of the OPs under sunlight. Moreover, satisfactory recoveries ranging from 93.3% to 103.6% were obtained for the real samples. The results indicated that the Ru(bpy)32+-ZIF-90-MnO2-based OP sensing platform is promising for applications in food safety and environmental monitoring.
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http://dx.doi.org/10.1039/d1ay00841bDOI Listing
June 2021

Highly efficient ammonia synthesis at low temperature over a Ru-Co catalyst with dual atomically dispersed active centers.

Chem Sci 2021 Apr 7;12(20):7125-7137. Epub 2021 Apr 7.

Department of Chemistry, Southern University of Science and Technology Shenzhen China.

The desire for a carbon-free society and the continuously increasing demand for clean energy make it valuable to exploit green ammonia (NH) synthesis that proceeds the electrolysis driven Haber-Bosch (eHB) process. The key for successful operation is to develop advanced catalysts that can operate under mild conditions with efficacy. The main bottleneck of NH synthesis under mild conditions is the known scaling relation in which the feasibility of N dissociative adsorption of a catalyst is inversely related to that of the desorption of surface N-containing intermediate species, which leads to the dilemma that NH synthesis could not be catalyzed effectively under mild conditions. The present work offers a new strategy introducing atomically dispersed Ru onto a single Co atom coordinated with pyrrolic N, which forms RuCo dual single-atom active sites. In this system the d-band centers of Ru and Co were both regulated to decouple the scaling relation. Detailed experimental and theoretical investigations demonstrate that the d-bands of Ru and Co both become narrow, and there is a significant overlapping of t and e orbitals as well as the formation of a nearly uniform Co 3d ligand field, making the electronic structure of the Co atom resemble that of a "free-atom". The "free-Co-atom" acts as a bridge to facilitate electron transfer from pyrrolic N to surface Ru single atoms, which enables the Ru atom to donate electrons to the antibonding π* orbitals of N, thus resulting in promoted N adsorption and activation. Meanwhile, H adsorbs dissociatively on the Co center to form a hydride, which can transfer to the Ru site to cause the hydrogenation of the activated N to generate NH ( = 1-4) intermediates. The narrow d-band centers of this RuCo catalyst facilitate desorption of surface *NH intermediates even at 50 °C. The cooperativity of the RuCo system decouples the sites for the activation of N from those for the desorption of *NH and *NH intermediates, giving rise to a favorable pathway for efficient NH synthesis under mild conditions.
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http://dx.doi.org/10.1039/d1sc00304fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8153211PMC
April 2021

miR-218-5p inhibits the malignant progression of glioma via targeting TCF12.

Tumori 2021 Jun 13:3008916211018263. Epub 2021 Jun 13.

The Fourth Department of Neurosurgery, Tangshan Gongren Hospital, Tangshan, China.

Several studies have shown the ability of transcription factor 12 (TCF12) to promote tumor malignant progression, but its function in glioma cells has not been fully elucidated. In this study, we analyzed the data from TCGA by bioinformatics and found that in glioma tissue, TCF12 was conspicuously highly expressed while miR-218-5p was significantly low-expressed. The downregulation of miR-218-5p was correlated with adverse prognosis in patients with glioma. miR-218-5p was found to be negatively associated with TCF12 by Pearson correlation analysis, and dual luciferase assay was employed to verify that miR-218-5p and TCF12 had a targeting relationship. qRT-PCR and Western blot assays were used to verify that the expression of TCF12 was regulated by its upstream regulator miR-218-5p. Moreover, cell experiments validated that overexpressed TCF12 could promote the proliferation, migration, and invasion of glioma cells and inhibit their apoptosis, whereas overexpressing miR-218-5p at the same time could reverse this phenomenon. Our study demonstrates the regulatory mechanism of the miR-218-5p/TCF12 axis in gliomas, which lays a foundation for searching for new therapeutic approaches for glioma.
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http://dx.doi.org/10.1177/03008916211018263DOI Listing
June 2021

Identification of macrophage related gene in colorectal cancer patients and their functional roles.

BMC Med Genomics 2021 Jun 13;14(1):159. Epub 2021 Jun 13.

Engineering Research Center for Medicine, Harbin University of Commerce, Harbin, 150076, China.

Background: Recent scientific research has enabled the identification of macrophages related-genes (MaRG), which play a key role in the control of the immune microenvironment in many human cancers. However, the functional role of MaRGs in human tumors is ill-defined. Herein, we aimed at bioinformatically exploring the molecular signatures of MaRGs in colorectal cancer.

Methods: A list of MaRGs was generated and their differential expression was analyzed across multiple datasets downloaded from the publicly available functional genomics database Gene Expression Omnibus. The weighted gene co-expression network analysis (WGCNA) was also applied to identify the partner genes of these MaRGs in colorectal cancer.

Results: After integration of the results from analyses of different datasets, we found that 29 differentially expressed MaRGs (DE-MaRGs) could be considered as CRC-related genes as obtained from the WGCNA analysis. These genes were functionally involved in positive regulation of DNA biosynthetic process and glutathione metabolism. Protein-protein interaction network analysis indicated that PDIA6, PSMA1, PRC1, RRM2, HSP90AB1, CDK4, MCM7, RFC4, and CCT5 were the hub MaRGs. The LASSO approach was used for validating the 29 MaRGs in TCGA-COAD and TCGA-READ data and the results showed that ten among the 29 genes could be considered as MaRGs significantly involved in CRC. The maftools analysis showed that MaRGs were mutated at varying degrees. The nomogram analysis indicated the correlation of these MaRGs with diverse clinical features of CRC patients.

Conclusions: Conclusively, the present disclosed a signature of MaRGs as potential key regulators involved in CRC pathogenesis and progression. These findings contribute not only to the understanding of the molecular mechanism of CRC pathogenesis but also to the development of adequate immunotherapies for CRC patients.
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http://dx.doi.org/10.1186/s12920-021-01010-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201885PMC
June 2021

In-situ investigation of dye pollutant adsorption performance on graphitic carbon nitride surface: ATR spectroscopy experiment and MD simulation insight.

J Hazard Mater 2021 Jun 3;418:126297. Epub 2021 Jun 3.

School of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu 610500, China.

The adsorption performances on graphitic carbon nitride (g-CN) surface were investigated for organic dye pollutants by both experimental and calculation methods. For experimental investigation, adsorption thermodynamics and kinetics results were in-situ obtained and evaluated. With [Formula: see text] by Langmuir modeling g-CN showed superior adsorption spontaneity of MB >MO. With linear and exponential modeling, gCN showed only adsorption process for MB but both diffusion and adsorption processes for MO. For simulation insight, all MB molecules but only parts of MO molecules were inclined to orient in parallel position at g-CN surface after optimization during low concentration. And both MB and MO molecules were inclined to orient in perpendicular position at g-CN surface after optimization during high concentration. Combined with experimental and calculation results, a molecular-orientation and force-dominance mechanism adsorption model are proposed to explain the surface interaction processes between dyes and g-CN. Electrostatic interaction and π-π stacking interaction were revealed to dominate for MB adsorption, and π-π stacking interaction and van der Waals force were revealed to dominate for MO adsorption. This work obtained 'localized' interfacial information and elucidated in-situ intermolecular interactions at g-CN interface, which can provide fundamental basis for operation removal of organic dye pollutants by g-CN.
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http://dx.doi.org/10.1016/j.jhazmat.2021.126297DOI Listing
June 2021

Efficacy and safety of ciprofol for the sedation/anesthesia in patients undergoing colonoscopy: Phase IIa and IIb multi-center clinical trials.

Eur J Pharm Sci 2021 Jun 8;164:105904. Epub 2021 Jun 8.

Department of Anesthesiology, West China Hospital, Sichuan University & The Research Units of West China (2018RU012), Chinese Academy of Medical Sciences, Chengdu, China. Electronic address:

Objective: Ciprofol is a new intravenous anesthetic agent similar to propofol that has the pharmacodynamic characteristics of a rapid rate of onset and recovery in pre-clinical experiments. The aims of the present clinical trials were to compare the efficacy and safety of ciprofol emulsion for sedation or general anesthesia during colonoscopy and to define optimal doses for a subsequent phase III clinical trial.

Methods: A phase IIa multi-center, open-label, non-randomized, positive control, dose-escalating study was performed to determine a recommended phase IIb dose (RP2D) of ciprofol to induce sedation or anesthesia in patients undergoing colonoscopy. Phase IIb was also a multi-center clinical trial, but the patients were randomized into 3 groups at a ratio of 1:1:1. It was a double-blinded, propofol controlled study that administered ciprofol 0.4 mg/kg (n = 31) and 0.5 mg/kg (n = 32) or propofol at 2.0 mg/kg (n = 31), with the aim of establishing the optimal dose of ciprofol. The primary endpoint was the colonoscopy success rate. Secondary endpoints were the duration of colonoscope insertion, recovery time, number of top-up doses needed, and the total dose of ciprofol or propofol required to maintain adequate sedation or anesthesia. In addition, we evaluated the satisfaction of sedation/anesthesia from the endoscopists, anesthetists and patients' points of view. Safety was assessed according to the incidence of AEs including serious AEs and drug related AEs and the assessment of vital signs, a 12-lead ECG and laboratory tests.

Results: In the phase IIa trial, the colonoscopy success rates in the 0.2-0.5 mg/kg ciprofol and propofol 2.0 mg/kg groups were 100% and all doses were safe and well tolerated. Ciprofol doses of 0.4 mg/kg and 0.5 mg/kg are recommended for subsequent IIb phases. In the phase IIb trial, a 100% success rate was reconfirmed in all the dosage groups. The mean time of colonoscope insertion in the ciprofol 0.4 mg/kg, ciprofol 0.5 mg/kg and propofol 2.0 mg/kg groups were 1.9, 1.5 and 1.5 min, the mean recovery times from colonoscope withdrawal were 6.1, 5.1, and 4.3 min, and the times to discharge were 11.8, 11.2 and 10.6 min, respectively. The satisfaction ratings of anesthetists in the ciprofol 0.5 mg/kg group (9.5 ± 0.8) were higher than in the ciprofol 0.4 mg/kg (9.2 ± 1.0) and propofol 2.0 mg/kg (9.2 ± 0.9) groups. The incidence of sedation and anesthesia-related AEs was highest in the propofol 2.0 mg/kg group (25.8%), followed by the ciprofol 0.5 mg/kg group (21.9%), and was least in the ciprofol 0.4 mg/kg group (16.1%) (P = 0.750).

Conclusions: Ciprofol was safe and well tolerated at doses ranging from 0.1 mg/kg to 0.5 mg/kg. Ciprofol 0.4-0.5 mg/kg induced equivalent sedation/anesthesia and had a similar safety profile to propofol 2.0 mg/kg during colonoscopy without producing serious AEs.
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http://dx.doi.org/10.1016/j.ejps.2021.105904DOI Listing
June 2021

STAT3-activated lncRNA XIST accelerates the inflammatory response and apoptosis of LPS-induced acute lung injury.

J Cell Mol Med 2021 Jun 11. Epub 2021 Jun 11.

Department of Thoracic Surgery, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.

Acute lung injury (ALI) is a severe lung respiratory failure characterized by high morbidity and mortality. Novel findings demonstrated the critical roles of long non-coding RNA (lncRNA) in ALI. Here, we tried to investigate the roles and potential mechanism of lncRNA X-inactive specific transcript (XIST) in ALI. Results illustrated that lncRNA XIST was up-regulated in the lipopolysaccharide (LPS)-induced ALI mice models and pulmonary endothelial cells. Biofunctional assays unveiled that knockdown of XIST repressed the inflammatory response and apoptosis in LPS-induced endothelial cells. Mechanistically, XIST acted as the miR-146a-5p sponge to positively regulate STAT3. Moreover, STAT3 combined the promoter region of XIST to accelerate the transcription, constituting the positive feedback loop of XIST/miR-146a-5p/STAT3 in ALI. Collectively, these findings suggested that XIST knockdown attenuates the LPS-induced ALI, providing a potential therapeutic target.
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http://dx.doi.org/10.1111/jcmm.16653DOI Listing
June 2021

Plant-Based Diet Index and Metabolic Risk in Men: Exploring the Role of the Gut Microbiome.

J Nutr 2021 Jun 10. Epub 2021 Jun 10.

Department of Nutrition, Harvard T.H. Chan School of Public Health, Boston, MA, USA.

Background: Healthy plant-based diet index (hPDI) is associated with a lower risk of cardiometabolic conditions, but its association as well as interactions with microbiome have not been elucidated.

Objectives: We aimed to investigate the interrelations between hPDI, gut microbiome, and cardiometabolic risk markers.

Methods: hPDI was derived from dietary assessments by a validated FFQ and was examined in relation to metagenomic profiles of 911 fecal samples collected from 303 men aged 71 ± 4 y with an average BMI (in kg/m2) of 25.2 ± 3.6 in the Men's Lifestyle Validation Study. Principal coordinate (PCo) analysis based on Bray-Curtis dissimilarity was conducted, and interactions between hPDI and PCo were examined by using a metabolic risk score composed of blood lipids, BMI, and glycated hemoglobin.

Results: After multivariable adjustment, hPDI was significantly associated with the relative abundance of 7 species and 9 pathways. In particular, higher hPDI was significantly associated with a higher relative abundance of Bacteroides cellulosilyticus and Eubacterium eligens, amino acid biosynthesis pathways (l-isoleucine biosynthesis I and III and l-valine biosynthesis), and the pathway of pyruvate fermentation to isobutanol. A favorable association between hPDI and the metabolic risk score was more pronounced among men with a higher PCo characterized by higher abundance of Bacteroides uniformis and lower abundance of Prevotella copri. At the individual species level, a similar interaction was also observed between hPDI and P. copri, as well as with Clostridium clostridioforme or Blautia hydrogenotrophica (all P-interaction < 0.01).

Conclusion: A greater adherence to a healthy plant-based diet by older men was associated with a microbial profile characterized by a higher abundance of multiple species, including B. cellulosilyticus and E. eligens, as well as pathways in amino acid metabolism and pyruvate fermentation. In addition, inverse associations between healthy plant-based diet and human metabolic risk may partially depend on microbial compositions.
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http://dx.doi.org/10.1093/jn/nxab175DOI Listing
June 2021

Advanced TeS-C Nanocomposites for High-Performance Lithium Ion Batteries.

Front Chem 2021 25;9:687392. Epub 2021 May 25.

Department of Chemistry and Biochemistry, University of Windsor, Windsor, ON, Canada.

This study is dedicated to expand the family of lithium-tellurium sulfide batteries, which have been recognized as a promising choice for future energy storage systems. Herein, a novel electrochemical method has been applied to engineer micro-nano TeS material, and it is found that TeS phases combined with multi-walled carbon nanotubes endow the as-constructed lithium-ion batteries excellent cycling stability and high rate performance. In the process of material synthesis, the sulfur was successfully embedded into the tellurium matrix, which improved the overall capacity performance. TeS was characterized and verified as a micro-nano-structured material with less Te and more S. Compared with the original pure Te particles, the capacity is greatly improved, and the volume expansion change is effectively inhibited. After the assembly of Li-TeS battery, the stable electrical contact and rapid transport capacity of lithium ions, as well as significant electrochemical performance are verified.
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http://dx.doi.org/10.3389/fchem.2021.687392DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8186662PMC
May 2021

lncRNA IUR upregulates miR-34a to inhibit pancreatic adenocarcinoma cell migratory and invasive abilities.

Oncol Lett 2021 Jul 29;22(1):567. Epub 2021 May 29.

Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.

The long non-coding RNA (lncRNA) imatinib-upregulated (IUR) has been recently reported as a tumor suppressor in leukemia. Preliminary microarray data revealed a downregulation of IUR in pancreatic adenocarcinoma (PAAD) and a positive correlation with microRNA-34a (miR-34a) expression. The present study aimed to investigate the role of IUR in PAAD. This study included samples from 58 patients with PAAD and the PAAD cell lines Capan-2 and HPAC. Reverse transcription quantitative PCR was performed to determine gene expression levels. Cell transfections were carried out to assess gene interactions between IUR, miR-34a and CD44. Transwell assays were performed to explore the effects of transfections on cell invasive and migratory abilities. The results demonstrated that IUR was downregulated in PAAD tissue compared with adjacent non-tumor tissue samples and that low expression levels of IUR correlated with poor survival in patients with PAAD. In PAAD tissue samples, the expression of IUR positively correlated with miR-34a expression but negatively correlated with CD44 expression, which is a target of miR-34a. In PAAD cells, overexpression of IUR resulted in miR-34a upregulation and CD44 downregulation. miR-34a overexpression did not affect the expression of IUR but downregulated CD44. In PAAD cells, overexpression of IUR and miR-34a led to decreased invasive and migratory abilities. However, CD44 overexpression played an opposite role and attenuated the effects of IUR and miR-34a overexpression. In conclusion, the results from this study demonstrated that IUR may upregulate miR-34a expression in order to inhibit PAAD cell migration and invasion by downregulating CD44.
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http://dx.doi.org/10.3892/ol.2021.12828DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8185698PMC
July 2021