Publications by authors named "Jun He"

1,556 Publications

  • Page 1 of 1

Trend of Sudden Unexpected Death in Epilepsy Incidence Rate in Rural West China.

Front Neurol 2021 24;12:735201. Epub 2021 Sep 24.

Department of Neurology, West China Hospital, Sichuan University, Chengdu, China.

To explore the trend of sudden unexpected death in epilepsy (SUDEP) incidence rate over time in rural west China. We scanned probable SUDEP patients from the epilepsy program between 2010 and 2019 in rural West China and performed a verbal autopsy for each eligible patient. We calculated the crude and sex-adjusted incidence rate of SUDEP per person-year over a calendar year and the year of follow-up. We calculated the incidence rate ratio with the Poisson model in STATA 12.0 and calculated the annual percentage change (APC) and average annual percentage change in Joinpoint Trend Analysis Software 4.8.0.1 to analyze the trend of SUDEP incidence rate. In 2010-2019, 44 probable SUDEPs were identified from 10,128 patients with a total person-year of 31,347. The crude and sex-adjusted incidence rates of SUDEP were 1.40 and 1.45%0. Twenty-five (56.8%) of the 44 probable SUDEPs had no generalized tonic-clonic seizure 3 months before their death. The incidence of probable SUDEP decreased significantly in the calendar year [APC = -11.7, 95% confidence interval (CI): -21.7 to -0.3] and in time of follow-up (average annual percentage change = -21.2, 95% CI: -34.3 to -5.4). Comparing the first 5 years in follow-up with the subsequent 3 years, the incidence rate of SUDEP decreased significantly (estimated incidence rate ratio = 0.4, 95% CI: 0.2 to 0.8). SUDEP happened to 1.4 cases per thousand patient-years in convulsive epilepsy in rural west China between 2010 and 2019. The incidence rate of SUDEP presented a downward trend over the time of follow-up.
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http://dx.doi.org/10.3389/fneur.2021.735201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8498108PMC
September 2021

NF-κB-dependent induction of porcine β-defensin 114 regulates intestinal epithelium homeostasis.

Int J Biol Macromol 2021 Oct 4;192:241-249. Epub 2021 Oct 4.

Institute of Animal Nutrition, Sichuan Agricultural University, Sichuan Province, Chengdu 611130, PR China; Key Laboratory of Animal Disease-resistant Nutrition, Sichuan Province, Chengdu 611130, PR China. Electronic address:

Intestinal epithelial cells (IECs) offer a primary physical barrier against commensal and pathogenic microorganisms in the gastrointestine. However, the influence of IECs on the development and regulation of mucosal immunity to infection is unknown. Here, we show that the porcine β-defensin 114 (PBD114) is an endotoxin-responsive gene expressed in IECs. Analysis on expression profiling of PBD114 gene using an infected porcine model and IPEC-J2 cells unveiled a pattern of induction in response to stimulation of various toll-like receptors (TLRs). By means of promoter analysis, PBD114 was found to be a NF-κB-dependent gene. Importantly, PBD114 suppresses endotoxin-induced inflammation and apoptosis in IECs through downregulation of two critical inflammation-associated signaling proteins, NF-kappa-B inhibitor alpha (IkB-α) and extracellular signal-regulated kinase1/2 (ERK1/2). PBD114 also suppresses inflammation and IEC apoptosis in mice exposed to bacterial endotoxins. Thus, we propose that TLR-activated NF-kB rapidly increases the expression of PBD114 that operates a feedback control of the NF-kB-dependent inflammation. The NF-kB-dependent induction of PBD114 may be a key event through which the mammalian host maintains intestinal epithelium homeostasis in response to various infections or diseases.
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http://dx.doi.org/10.1016/j.ijbiomac.2021.09.163DOI Listing
October 2021

Femtosecond laser point-by-point inscription of an ultra-weak fiber Bragg grating array for distributed high-temperature sensing.

Opt Express 2021 Sep;29(20):32615-32626

Ultra-weak fiber Bragg grating (UWFBG) arrays are key elements for constructing large-scale quasi-distributed sensing networks for structural health monitoring. Conventional methods for creating UWFBG arrays are based on in-line UV exposure during fiber drawing. However, the UV-induced UWFBG arrays cannot withstand a high temperature above 450 °C. Here, we report for the first time, to the best of our knowledge, a new method for fabricating high-temperature-resistant UWFBG arrays by using a femtosecond laser point-by-point (PbP) technology. UWFBGs with a low peak reflectivity of ∼ - 45 dB (corresponding to ∼ 0.0032%) were successfully fabricated in a conventional single-mode fiber (SMF) by femtosecond laser PbP inscription through fiber coating. Moreover, the influences of grating length, laser pulse energy, and grating order on the UWFBGs were studied, and a grating length of 1 mm, a pulse energy of 29.2 nJ, and a grating order of 120 were used for fabricating the UWFBGs. And then, a long-term high-temperature annealing was carried out, and the results show that the UWFBGs can withstand a high temperature of 1000 °C and have an excellent thermal repeatability with a sensitivity of 18.2 pm/°C at 1000 °C. A UWFBG array consisting of 200 identical UWFBGs was successfully fabricated along a 2 m-long conventional SMF with an interval of 10 mm, and interrogated with an optical frequency domain reflectometer (OFDR). Distributed high-temperature sensing up to 1000 °C was demonstrated by using the fabricated UWFBG array and OFDR demodulation. As such, the proposed femtosecond laser-inscribed UWFBG array is promising for distributed high-temperature sensing in hash environments, such as aerospace vehicles, nuclear plants, and smelting furnaces.
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http://dx.doi.org/10.1364/OE.437479DOI Listing
September 2021

Strong couplings between magnetic quantum emitters and subwavelength all-dielectric resonators with whispering gallery modes.

Opt Express 2021 Aug;29(16):26028-26038

Here we theoretically investigate the coherent interactions between the quantum emitters with magnetic dipole transitions and subwavelength all-dielectric resonators of whispering gallery modes (WGMs). We extend a semi-analytical method which can efficiently calculate the far-field spectrum of a general hybrid system. Then, a subwavelength sphere with refractive index around n = 3.5 is chosen as the dielectric resonator. Due to the high magnetic field enhancements of the WGMs of the sphere, strong couplings between magnetic quantum emitters and subwavelength WGMs can occur, where a clear Rabi splitting appears on the extinction spectrum of the hybrid system. The match between the relaxation times of the WGMs and emitters are important to efficiently achieve a strong enough coupling. The other parameters including the order of a WGM, the radius, the refractive index, the transition dipole moment and excitation intensity are also important factors that can affect the couplings. Our results pave the way for strong interactions between light and magnetic emitters mediated by subwavelength all-dielectric resonators.
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http://dx.doi.org/10.1364/OE.434586DOI Listing
August 2021

Gut microbiota changes in preeclampsia, abnormal placental growth and healthy pregnant women.

BMC Microbiol 2021 Oct 4;21(1):265. Epub 2021 Oct 4.

Changsha Hospital for Maternal and Child Health Care, Hunan, China.

Background: Preeclampsia (PE) is a condition of high blood pressure that is usually concurrent with proteinuria in pregnancy. PE complicates the management of both maternal and fetal health and contributes to most adverse pregnancy outcomes, but the mechanism underlying the development of PE remains unclear. In this study, we performed a case-control study to compare the gut microbiota of PE (n = 26), abnormal placental growth (APG, n = 25) and healthy pregnant women (n = 28) and analyzed the potential pathogenic role of gut microbiota in PE progression.

Results: The clinical pathophysiological state did not affect the bacterial diversity, while the compositions of the gut microbiota were significantly altered in both the PE and APG groups compared with healthy pregnant women. At the phylum level, TM7 was significantly increased in women with APG. Heterogeneity was observed at the genus level, especially in genera with positive LDA scores, suggesting the stage-dependent effect of gut microbiota on the development of PE. The beneficial bacterium Lactobacillus was markedly depleted in the PE and APG groups but was only correlated with blood pressure (BP) and proteinuria levels in the PE group. Two different bacterial taxa belonged to Lactobacillus showed different correlations (OTU255 and OTU784 were significantly related to PE and APG, respectively).

Conclusions: Our results indicated that shifts in the gut microbiota might occur from the early stages of the development of PE, which is of possible etiological and therapeutic importance.
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http://dx.doi.org/10.1186/s12866-021-02327-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8489045PMC
October 2021

Integrated Analysis of Liver Transcriptome, miRNA, and Proteome of Chinese Indigenous Breed Ningxiang Pig in Three Developmental Stages Uncovers Significant miRNA-mRNA-Protein Networks in Lipid Metabolism.

Front Genet 2021 16;12:709521. Epub 2021 Sep 16.

College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.

Liver is an important metabolic organ of mammals. During each transitional period of life, liver metabolism is programmed by a complex molecular regulatory system for multiple physiological functions, many pathways of which are regulated by hormones and cytokines, nuclear receptors, and transcription factors. To gain a comprehensive and unbiased molecular understanding of liver growth and development in Ningxiang pigs, we analyzed the mRNA, microRNA (miRNA), and proteomes of the livers of Ningxiang pigs during lactation, nursery, and fattening periods. A total of 22,411 genes (19,653 known mRNAs and 2758 novel mRNAs), 1122 miRNAs (384 known miRNAs and 738 novel miRNAs), and 1123 unique proteins with medium and high abundance were identified by high-throughput sequencing and mass spectrometry. We show that the differences in transcriptional, post-transcriptional, or protein levels were readily identified by comparing different time periods, providing evidence that functional changes that may occur during liver development are widespread. In addition, we found many overlapping differentially expressed genes (DEGs)/differentially expressed miRNAs (DEMs)/differentially expressed proteins (DEPs) related to glycolipid metabolism in any group comparison. These overlapping DEGs/DEMs/DGPs may play an important role in functional transformation during liver development. Short Time-series Expression Miner (STEM) analysis revealed multiple expression patterns of mRNA, miRNA, and protein in the liver. Furthermore, several diverse key Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including immune defense, glycolipid metabolism, protein transport and uptake, and cell proliferation and development, were identified by combined analysis of DEGs and DGPs. A number of predicted miRNA-mRNA-protein pairs were found and validated by qRT-PCR and parallel reaction monitoring (PRM) assays. The results provide new and important information about the genetic breeding of Ningxiang pigs, which represents a foundation for further understanding the molecular regulatory mechanisms of dynamic development of liver tissue, functional transformation, and lipid metabolism.
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http://dx.doi.org/10.3389/fgene.2021.709521DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8481880PMC
September 2021

Job Dissatisfaction Mediated the Associations Between Work Stress and Mental Health Problems.

Front Psychiatry 2021 16;12:711263. Epub 2021 Sep 16.

Department of Social Medicine and Health Management, Xiangya School of Public Health, Central South University, Changsha, China.

This study aimed to explore the relationships and the underlying mechanisms between work stress and mental health problems, and potential mediation effects through job dissatisfaction in a working population. A large population-based study among workers in China was conducted. The self-reported scales of assessing job dissatisfaction and work stress were included in the questionnaire. Generalized Anxiety Disorder-2 and Patient Health Questionnaire-2 were used for assessment of mental health. Univariate logistic regression was conducted to test the associations between work stress and mental health. Path analysis was conducted to test the proposed mediation model. Of the 6,190 included employees, 27.72% reported that they perceived work stress, 14.84% of them reported that they were not satisfied with their work, 5.01% of the employees reported depressive symptoms, and 3.75% of the employees reported anxiety symptoms. The results of univariate logistic regression showed that employees who perceived work stress were more likely to report anxiety symptoms (adjusted odds ratio (AOR) = 2.78; 95% CI: 2.03-3.79) or depressive symptoms (AOR = 1.61; 95% CI: 1.22-2.12). The path analysis showed that work stress was positively associated with job dissatisfaction. Job dissatisfaction mediated the relationship between work stress and mental health problems among Chinese working adults. This study suggests the importance of psychosocial work environment for mental health among Chinese working adults. Work dissatisfaction is a stressor that may induce negative consequences on the mental health among Chinese workers. Interventions to help workers with stress management may be beneficial for their mental health.
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http://dx.doi.org/10.3389/fpsyt.2021.711263DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8483558PMC
September 2021

Combined microbial transcript and metabolic analysis reveals the different roles of hydrochar and biochar in promoting anaerobic digestion of waste activated sludge.

Water Res 2021 Sep 24;205:117679. Epub 2021 Sep 24.

Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China; Shanghai Technical Service Platform for Pollution Control and Resource Utilization of Organic Wastes, Shanghai 200438, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address:

Hydrothermal pretreatment of waste activated sludge (WAS) could eliminate the rate limiting step of anaerobic digestion (AD) -hydrolysis. However, the high organic loading rate may cause acid accumulation, thus leading to an unstable system. This study compared the effect of different hydrochar (HC2-260°C and HC3-320°C) and biochar (BC5-500°C and BC7-700°C) on AD of hydrothermal pretreated WAS (HPS). Results demonstrated that hydrochar was superior to biochar in the methane yield and production rate, especially HC2. HC2 had the highest surface oxygen-containing functional groups that could facilitate direct interspecies electron transfer (DIET). The enhanced methane yield was related with the increased protein utilization, and hydrochar and biochar enriched different microbes related to protein degradation. Metabolomic analysis showed the significantly changed metabolites induced by hydrochar and biochar were involved in fatty acids and amino acids-related metabolism, indicating the rapid conversion of intermediated products, which was consistent with the microbial community structure results. Hydrochar and biochar also induced upregulation of metabolites related to microbial metabolic activity and extracellular electron transfer. Although biochar induced the same metabolic changes, the alterations of these metabolites were weaker than those of hydrochar. The results of this study offered new insights into the molecular mechanisms of enhanced AD of HPS by hydrochar and biochar.
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http://dx.doi.org/10.1016/j.watres.2021.117679DOI Listing
September 2021

Quality Improvement Tools in Total Joint Arthroplasty: A Systematic Review.

J Surg Orthop Adv 2021 ;30(3):125-130

Department of Orthopaedic Surgery, University of Alabama at Birmingham Hospital, Birmingham, Alabama.

Systems review and quality improvement (QI) is a significant need within orthopaedic surgery. The focus of this paper is to systematically review QI principles utilized in total joint arthroplasty to determine most successful QI tools. A systematic search on MEDLINE/Pubmed, Embase, Cochrane Library and other sources was conducted from September 1991 through October 2018. The three primary improved outcomes from each article were recorded along with the date, author and subspecialty. Thirty-four eligible studies related to joint arthroplasty were identified for inclusion in the systematic review. The most common outcomes that were improved in these publications were: length of stay (LOS), cost, medication management, and patient education. Lean, clinical care pathways (CCP), plan-do-check-act (PDCA), and shared decision-making improved those metrics. Four metrics were found that were consistently improved by certain quality improvement tools: LOS, cost, medication management, and patient education. Further research is warranted to continue to build a framework for quality improvement in orthopaedic surgery. (Journal of Surgical Orthopaedic Advances 30(3):125-130, 2021).
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October 2021

LC-MS/MS-based metabolomics and sensory evaluation characterize metabolites and texture of normal and spoiled dry-cured hams.

Food Chem 2021 Sep 17;371:131156. Epub 2021 Sep 17.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, China; Key Laboratory of Animal Protein Food Processing Technology of Zhejiang Province, Ningbo University, Ningbo 315211, China. Electronic address:

The spoiled dry-cured ham commonly shows unpleasant taste and odour. To deepen the understanding in the formation mechanism of unpleasant taste in spoiled ham, sensory attributes, texture parameters, protein degradation, metabolites were investigated between normal and spoiled hams; the relationship between the sensory quality and metabolites of dry-cured ham was further evaluated by partial least square discriminant analysis (PLS-DA). The scores of richness and overall acceptance were significantly lower in spoiled ham, and more than 12.5-fold values in adhesiveness were found in spoiled ham than normal ham. Myofibrillar proteins including actin, troponin-T and myosin light chain showed excessive degradation in spoiled ham. Forty-two kinds of metabolites mainly derived from protein degradation were identified by LC-MS/MS, and amino acid derivatives and oligopeptides were the key components to distinguish spoiled and normal hams demonstrated by PLS-DA. Purine metabolism, pyrimidine metabolism and protein degradation were the main metabolism pathways in spoiled ham.
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http://dx.doi.org/10.1016/j.foodchem.2021.131156DOI Listing
September 2021

Analysis Model of the Impact of Refined Intervention in Operating Room on Patients' Recovery Quality and Complications after Thoracic Surgery Based on Deep Neural Network.

Authors:
Jun He Jing Wen

J Healthc Eng 2021 17;2021:7006120. Epub 2021 Sep 17.

Department of Medical Engineering, Tangshan Workers' Hospital, Hebei 063000, China.

To improve the nursing effect in patients after thoracic surgery, this paper proposes a refined intervention method in the operating room based on traditional operating room nursing and applies this method to the nursing of patients after thoracic surgery. Moreover, this paper improves the traditional neural network algorithm and uses the deep neural network algorithm to process test data. In addition, it includes patients accepted by the hospital as samples for test analysis and formulates detailed intervention methods for the operating room. Finally, this paper collects the corresponding test data by setting up test and control groups and visually displays the data using mathematical statistics. The statistical parameters of the experiment in this paper include the quality of recovery, complications, satisfaction score, and recovery effect. The comparative test shows that the refined intervention in the operating room based on the neural network proposed in this paper can achieve a certain effect in the postoperative nursing of thoracic surgery, effectively promote the quality of recovery, and reduce the possibility of complications.
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http://dx.doi.org/10.1155/2021/7006120DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464422PMC
September 2021

Low Birth Weight Disturbs the Intestinal Redox Status and Mitochondrial Morphology and Functions in Newborn Piglets.

Animals (Basel) 2021 Aug 31;11(9). Epub 2021 Aug 31.

Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China.

Low birth-weight (LBW) neonates exhibit a lower growth rate and impaired intestinal development. However, the reasons for abnormal development of small intestine in LBW piglets have not been widely studied. The present study focused on the redox status and mitochondrial morphology and functions of the small intestine in LBW newborn piglets. Ten newborn normal birth-weight (NBW) piglets and LBW piglets from 10 primiparous sows with the same parturition day were selected and sampled immediately without sucking colostrum. The small intestine tissues were collected and measured. Compared with NBW newborn piglets, LBW newborn piglets had a significantly decreased length and weight of the small intestine ( < 0.05) as well as the villus height/crypt depth (V/C) index in the jejunum ( < 0.05). Furthermore, LBW piglets had a lower gene expression of tight junction protein zonula occluden-1 (), , antioxidant enzyme catalase (), glutathione peroxidase () and heme oxygenase-1 () in jejunum ( < 0.05). Meanwhile, LBW induced mitochondrial vacuolation and significantly decreased the mRNA expression of PPARγ coactivator-1α () ( < 0.05) and tended to decrease the expression of cytochrome coxidase IV () ( = 0.07) and cytochrome C () ( = 0.08). In conclusion, LBW newborn piglets showed an abnormal development of the small intestine and disturbed redox status, and this may be caused by impaired morphology and the functions of mitochondria in the jejunum.
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http://dx.doi.org/10.3390/ani11092561DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469446PMC
August 2021

Spatiotemporal Regulation and Functional Analysis of Circular RNAs in Skeletal Muscle and Subcutaneous Fat during Pig Growth.

Biology (Basel) 2021 Aug 30;10(9). Epub 2021 Aug 30.

College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.

Recently, thousands of circular RNAs have been reported in different pig breeds. However, researches on the temporal and spatial expression patterns of circRNA over the period of animal growth are limited. Here, we systematically analyzed circRNAs in skeletal muscle and subcutaneous fat in four growth time points (30 days, 90 days, 150 days and 210 days after birth) of a Chinese native pig breed, Ningxiang pigs. A total of 1171 differentially expressed (DE) circRNAs between muscle and fat were identified, including 562 upregulated and 609 downregulated circRNAs. KEGG pathway enrichment analysis of these DE circRNAs revealed that host genes were mainly involved in glycogen metabolism signaling pathways, muscle development signaling pathways such as ErbB pathway and adipocytokine signaling pathways and AMPK signaling pathways and fatty acid biosynthesis. The circRNAs have striking spatiotemporal specificity in the form of dynamic expression at 90 d. Short Time-Series Expression Miner analysis showed multiple model spectra that are significantly enriched with time changes in muscle and fat. Our findings provide new ideas and perspectives about the role of circular RNAs and their targeting relations with mRNA and miRNA in skeletal muscle and fat tissue during pig growth.
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http://dx.doi.org/10.3390/biology10090841DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8465536PMC
August 2021

Current progress on catalytic oxidation of toluene: a review.

Environ Sci Pollut Res Int 2021 Sep 27. Epub 2021 Sep 27.

Department of Chemical and Environmental Engineering, University of Nottingham Ningbo China, Ningbo, People's Republic of China.

Toluene is one of the pollutants that are dangerous to the environment and human health and has been sorted into priority pollutants; hence, the control of its emission is necessary. Due to severe problems caused by toluene, different techniques for the abatement of toluene have been developed. Catalytic oxidation is one of the promising methods and effective technologies for toluene degradation as it oxidizes it to CO and does not deliver other pollutants to the environment. This paper highlights the recent progressive advancement of the catalysts for toluene oxidation. Five categories of catalysts, including noble metal catalysts, transition metal catalysts, perovskite catalysts, metal-organic frameworks (MOFs)-based catalysts, and spinel catalysts reported in the past half a decade (2015-2020), are reviewed. Various factors that influence their catalytic activities, such as morphology and structure, preparation methods, specific surface area, relative humidity, and coke formation, are discussed. Furthermore, the reaction mechanisms and kinetics for catalytic oxidation of toluene are also discussed.
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http://dx.doi.org/10.1007/s11356-021-16492-9DOI Listing
September 2021

Adult adrenal neuroblastoma: A case report.

Mol Clin Oncol 2021 Nov 1;15(5):225. Epub 2021 Sep 1.

Department of Urology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China.

Adrenal neuroblastoma (NB) is very rare in adults. According to the literature, <100 cases have been reported worldwide to date, with >90% of the patients aged <10 years. As the early symptoms of the disease are not obvious, distant metastasis has often already occurred when the patients develop clinical symptoms. This lack of obvious symptoms may lead to misdiagnosis and inadequate treatment. Imaging and laboratory examinations are crucial for the diagnosis of NB, but reaching a definitive diagnosis prior to surgery is challenging, as the final diagnosis ultimately depends on histopathological examination. The aim of the present study was to report the rare case of a 40-year-old woman with adrenal left NB who underwent tumor resection. No tumor recurrence was observed at the 3-month and 1-year postoperative follow-up, but a repeat computed tomography at the 3-year postoperative follow-up indicated metastases; the patient refused further treatment and eventually succumbed to the disease within 1 month. The aim of the present case was to emphasize the importance of individualized therapy and long-term, close follow-up of the patients. The clinical characteristics and treatment of this case of adrenal NB were also summarized and analyzed in order to raise clinical awareness of this rare disease.
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http://dx.doi.org/10.3892/mco.2021.2378DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447177PMC
November 2021

Comparison of Sutureless Suture Partial Nephrectomy for Clinical T1 Renal Cell Carcinoma: A Meta-Analysis of Retrospective Studies.

Front Oncol 2021 2;11:713645. Epub 2021 Sep 2.

Department of Urology, Affiliated Hospital of Guizhou Medical University, Guiyang, China.

Background: Partial nephrectomy (PN) is the recommended treatment for T1 renal cell carcinoma (RCC). Compared with suture PN, sutureless PN reduces the difficulty and time of operation, but the safety and feasibility have been controversial. This meta-analysis was conducted to compare the function and perioperative outcomes of suture and sutureless PN for T1 RCC.

Methods: Systematic literature review was performed up to April 2021 using multiple databases to identify eligible comparative studies. According to the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) criteria, identification and selection of the studies were conducted. Meta-analysis was performed for studies comparing suture to sutureless PN for both T1a and T1b RCC. In addition, subgroup analysis was performed on operation time, warm ischemia time, estimated blood loss, and postoperative complications. Sensitivity analysis was used in analysis with high heterogeneity (operation time and estimated blood loss).

Results: Eight retrospective studies were included with a total of 1,156 patients; of the 1,156 patients, 499 received sutureless PN and 707 received suture PN. The results showed that sutureless PN had shorter operative time (I = 0%, < 0.001), warm ischemia time (I = 97.5%, < 0.001), and lower clamping rate (I = 85.8%, = 0.003), but estimated blood loss (I = 76.6%, = 0.064) had no difference. In the comparison of perioperative outcomes, there was no significant difference in postoperative complications (I = 0%, = 0.999), positive surgical margins (I = 0%, = 0.356), postoperative estimated glomerular filtration rat (eGFR) (I = 0%, = 0.656), and tumor recurrence (I = 0%, = 0.531).

Conclusions: In T1a RCC with low RENAL score, sutureless PN is a feasible choice, whereas it should not be overestimated in T1b RCC.
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http://dx.doi.org/10.3389/fonc.2021.713645DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445026PMC
September 2021

Novel Monoclonal Antibodies and Recombined Antibodies Against Variant SARS-CoV-2.

Front Immunol 2021 30;12:715464. Epub 2021 Aug 30.

The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.

The mutants resulted from the ongoing SARS-CoV-2 epidemic have showed resistance to antibody neutralization and vaccine-induced immune response. The present study isolated and identified two novel SARS-CoV-2 neutralizing antibodies (nAbs) from convalescent COVID-19 patients. These two nAbs (XG81 and XG83) were then systemically compared with nine nAbs that were reconstructed by using published data, and revealed that, even though these two nAbs shared targeting epitopes on spike protein, they were different from any of the nine nAbs. Compared with XG81, XG83 exhibited a higher RBD binding affinity and neutralization potency against wild-typed pseudovirus, variant pseudoviruses with mutated spike proteins, such as D614G, E484Q, and A475V, as well as the authentic SARS-CoV-2 virus. To explore potential broadly neutralizing antibodies, heavy and light chains from all 18 nAbs (16 published nAbs, XG81 and XG83) were cross-recombined, and some of the functional antibodies were screened and studied for RBD binding affinity, and neutralizing activity against pseudovirus and the authentic SARS-CoV-2 virus. The results demonstrated that several recombined antibodies had a more potent neutralization activity against variant pseudoviruses compared with the originally paired Abs. Taken together, the novel neutralizing antibodies identified in this study are a likely valuable addition to candidate antibody drugs for the development of clinical therapeutic agents against SARS-CoV-2 to minimize mutational escape.
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http://dx.doi.org/10.3389/fimmu.2021.715464DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8442604PMC
September 2021

Potency of the pandemic on air quality: An urban resilience perspective.

Sci Total Environ 2021 Sep 10;805:150248. Epub 2021 Sep 10.

College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua 321004, China.

Since the outbreak of COVID-19 pandemic, the lockdown policy across the globe has brought improved air quality while fighting against the coronavirus. After the closure, urban air quality was subject to emission reduction of air pollutants and rebounded to the previous level after the potency period of recession. Different response patterns exhibit divergent sensitivities of urban resilience in regard to air pollution. In this paper, we investigate the post-lockdown AQI values of 314 major cities in China to analyse their differential effects on the influence factors of urban resilience. The major findings of this paper include: 1) Cities exhibit considerable range of resilience with their AQI values which are dropped by 21.1% per day, took 3.97 days on average to reach the significantly decreased trough point, and reduced by 49.3% after the lockdown initiatives. 2) Mega cities and cities that locate as the focal points of transportation for nearby provinces, together with those with high AQI values, were more struggling to maintain a good air quality with high rebounds. 3) Urban resilience shows divergent spatial sensitivities to air pollution controls. Failing to consider multi-dimensional factors besides from geomorphological and economical activities could lead to uneven results of environmental policies. The results unveil key drivers of urban air pollution mitigation, and provide valuable insights for prediction of air quality in response to anthropogenic interference events under different macro-economic contexts. Research findings in this paper can be adopted for prevention and management of public health risks from the perspective of urban resilience and environmental management in face of disruptive outbreak events in future.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150248DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8428995PMC
September 2021

Clinical efficacy of neurointerventional arterial catheter thrombolysis in the treatment of cerebral infarction.

Minerva Surg 2021 Sep 15. Epub 2021 Sep 15.

Department of Emergency Medicine, Taizhou First People's Hospital, Taizhou, China -

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http://dx.doi.org/10.23736/S2724-5691.21.09078-XDOI Listing
September 2021

The nonlinear optical transition bleaching in tellurene.

Nanoscale 2021 Oct 1;13(37):15882-15890. Epub 2021 Oct 1.

Hunan Key Laboratory of Nanophotonics and Devices, School of Physics and Electronics, Central South University, 932 South Lushan Road, Changsha, Hunan 410083, P. R. China.

To date, outstanding linear and nonlinear optical properties of tellurene, caused by multiple two-dimensional (2D) phases and optical anisotropy, have attracted considerable interest for potential nanophotonics applications. In this work, the ultrafast nonlinear optical (NLO) properties of α-tellurene have been studied -scan and pump-probe techniques at a broadband spectral region. Typical saturable absorption and band filling effects are observed in tellurene due to the Pauli exclusion principle. Analysis using density functional theory (DFT) computation shows the enhancements in NLO response within the ultraviolet-visible absorption spectral region are owing to the increased optical intraband transition in tellurene. Moreover, the effects of varying the photon energy of the probe pulse were explored. Our results indicated that probe pulses with higher photon energies can make smaller differential transmission signal, this effect is found to be negatively correlated with calculated joint density of states (JDOS). These results offer insights into the intrinsic photophysics of 2D tellurene, driving its applications in photonic and optoelectronic fields.
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http://dx.doi.org/10.1039/d1nr03639dDOI Listing
October 2021

The Use of CRISPR/Cas9 as a Tool to Study Human Infectious Viruses.

Front Cell Infect Microbiol 2021 27;11:590989. Epub 2021 Aug 27.

Changsha Hospital for Maternal and Child Health Care of Hunan Normal University, Changsha, China.

Clustered regularly interspaced short palindromic repeats (CRISPR) systems are a set of versatile gene-editing toolkit that perform diverse revolutionary functions in various fields of application such as agricultural practices, food industry, biotechnology, biomedicine, and clinical research. Specially, as a novel antiviral method of choice, CRISPR/Cas9 system has been extensively and effectively exploited to fight against human infectious viruses. Infectious diseases including human immunodeficiency virus (HIV), hepatitis B virus (HBV), human papillomavirus (HPV), and other viruses are still global threats with persistent potential to probably cause pandemics. To facilitate virus removals, the CRISPR/Cas9 system has already been customized to confer new antiviral capabilities into host animals either by modifying host genome or by directly targeting viral inherent factors in the form of DNA. Although several limitations and difficulties still need to be conquered, this technology holds great promises in the treatment of human viral infectious diseases. In this review, we will first present a brief biological feature of CRISPR/Cas9 systems, which includes a description of CRISPR/Cas9 structure and composition; thereafter, we will focus on the investigations and applications that employ CRISPR/Cas9 system to combat several human infectious viruses and discuss challenges and future perspectives of using this new platform in the preclinical and clinical settings as an antiviral strategy.
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http://dx.doi.org/10.3389/fcimb.2021.590989DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8430244PMC
August 2021

The effect of CYP7B1 polymorphisms on the risk of coronary heart disease in Hainan Han population.

BMC Med Genomics 2021 09 7;14(1):220. Epub 2021 Sep 7.

Key Laboratory of Molecular Mechanism and Intervention Research for Plateau Diseases of Tibet Autonomous Region, School of Medicine, Xizang Minzu University, Xianyang, 712082, Shaanxi, China.

Background: Coronary heart disease (CHD) is the leading cause of human death worldwide. Genetic factors play an important role in the occurrence of CHD. Our study is designed to investigate the influence of CYP7B1 polymorphisms on CHD risk.

Methods: In this case-control study, 508 CHD patients and 510 healthy individuals were recruited to determine the correlation between CYP7B1 polymorphisms (rs7836768, rs6472155, and rs2980003) and CHD risk. The associations were evaluated by computing odds ratios (OR) and 95% confidence intervals (CI) with logistic regression analysis. The association between SNP-SNP interaction and CHD susceptibility was carried out by multifactor dimensionality reduction analyses.

Results: Our study found that rs6472155 is significantly associated with an increased risk of CHD in age > 60 years (OR 2.20, 95% CI = 1.07-4.49, p = 0.031), women (OR 3.17, 95% CI = 1.19-8.44, p = 0.021), and non-smokers (3.43, 95% CI = 1.16-10.09, p = 0.025). Rs2980003 polymorphism has a lower risk of CHD in drinkers (OR 0.47, 95% CI = 0.24-0.91, p = 0.025). Further analyses based on false-positive report probability validated these significant results. Besides, it was found that rs6472155 polymorphism was associated with uric acid level (p = 0.034).

Conclusion: Our study indicated that CYP7B1 polymorphisms are related to the risk of CHD, which provides a new perspective for prevent of CHD.
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http://dx.doi.org/10.1186/s12920-021-01067-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422734PMC
September 2021

Crucial Mutations of Spike Protein on SARS-CoV-2 Evolved to Variant Strains Escaping Neutralization of Convalescent Plasmas and RBD-Specific Monoclonal Antibodies.

Front Immunol 2021 17;12:693775. Epub 2021 Aug 17.

The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China (USTC), Hefei, China.

Small number of SARS-CoV-2 epidemic lineages did not efficiently exhibit a neutralization profile, while single amino acid mutation in the spike protein has not been confirmed in altering viral antigenicity resulting in immune escape. To identify crucial mutations in spike protein that escape humoral immune response, we evaluated the cross-neutralization of convalescent plasmas and RBD-specific monoclonal antibodies (mAbs) against various spike protein-based pseudoviruses. Three of 24 SARS-CoV-2 pseudoviruses containing different mutations in spike protein, including D614G, A475V, and E484Q, consistently showed an altered sensitivity to neutralization by convalescent plasmas. A475V and E484Q mutants are highly resistant to neutralization by mAb B38 and 2-4, suggesting that some crucial mutations in spike protein might evolve SARS-CoV-2 variants capable of escaping humoral immune response.
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http://dx.doi.org/10.3389/fimmu.2021.693775DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416052PMC
September 2021

Distribution Evaluation of Tenofovir in the Breast Milk of Mothers With HBeAg-Positive Chronic HBV Infection After Treatment With Tenofovir Alafenamide and Tenofovir Disoproxil Fumarate by a Sensitive UPLC-MS/MS Method.

Front Pharmacol 2021 13;12:734760. Epub 2021 Aug 13.

Department of Obstetrics and Gynecology, The Second Hospital of Nanjing, Nanjing University of Chinese Medicine, Nanjing, China.

Tenofovir alafenamide (TAF) is a novel prodrug of tenofovir (TFV) that has been approved for the treatment of chronic hepatitis B virus (HBV) infection. It has greater plasma stability and more favorable renal safety than tenofovir disoproxil fumarate (TDF), the first approved oral prodrug of TFV. However, the distribution of TFV in the breast milk of mothers treated with TAF is still unclear. In this study, sixteen participants with chronic HBV infection were enrolled and received antiretroviral therapy with 25 mg of TAF or 300 mg of TDF daily from 24 to 28 weeks of gestation until the 4th week postpartum. For the first time, the distribution of TFV in the breast milk of mothers with chronic HBV infection treated with TAF and its difference from TDF were evaluated by using a sensitive UPLC-MS/MS method. Chromatographic separation was achieved on a Waters ACQUITY UPLC BEH C18 column (1.7 µm 2.1 × 100 mm). Mass spectrometry analysis was performed in positive electrospray ionization mode and multiple reaction monitoring (MRM) conditions of transitions m/z 288.1→176.2 for TFV. This method was linear from 0.5 to 500 ng/ml. Surprisingly, on the third postpartum day, the median Cmax of TFV in the breast milk was much higher in the mothers treated with TAF (101.2 ng/ml) than TDF (21.6 ng/ml) at a similar Tmax of 4 h. Accordingly, the median AUC0-8 value was 755.6 ng h/mL in the mothers taking TAF, which was at a 5-fold higher level than TDF. The concentration of TFV in the breast milk of mothers in both groups decreased with increasing lactation time. These data indicated that there was a relatively higher exposure of TFV in the breast milk of mothers taking TAF, despite the lower dosage compared to TDF. This study provides support for further evaluating the safety of breastfeeding after the administration of TAF and TDF.
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http://dx.doi.org/10.3389/fphar.2021.734760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8414412PMC
August 2021

Phenol promoted caproate production via two-stage batch anaerobic fermentation of organic substance with ethanol as electron donor for chain elongation.

Water Res 2021 Oct 27;204:117601. Epub 2021 Aug 27.

Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai, 200433, China; Shanghai Technical Service Platform for Pollution Control and Resource Utilization of Organic Wastes, Shanghai, 200438, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai, 200092, China. Electronic address:

The conversion of organic wastes/wastewater into medium chain fatty acids (MCFAs) such as caproate has attracted much attention, while the effects of toxic compounds on the process have rarely been studied. The present study investigated the effects of phenol (0-1.5 g/L), which is a toxicant and present in various organic wastes, on the caproate production in the chain elongation (CE) process with ethanol as electron donor via two-stage batch anaerobic fermentation of glucose. The results showed phenol ≤ 1 g/L did not affect short chain fatty acids (SCFAs) production, while 1 g/L phenol increased caproate production by 59.9% in the following CE process. The higher selectivity of caproate and higher consumption of ethanol contributed to the higher caproate production at 1 g/L phenol. It was also shown 1 g/L phenol had more positive effect on CE of butyrate than acetate. 1.5 g/L phenol inhibited both SCFAs production and CE processes. 16S rRNA genes analysis showed phenol had slight effect on the microbial communities for SCFAs production, while it obviously changed the dominant microbes in CE process. For CE process, metagenomic analysis was further conducted and phenol mainly affected fatty acid biosynthesis (FAB) pathway, but not reverse β-oxidization (RBO) pathway. 1 g/L phenol increased the abundances of genes in FAB pathway, which could be related with the higher caproate production. Genome reconstruction identified the dominant microbial species in CE process, which were changed with different concentrations of phenol. Most of the dominant species were new microbial species potentially involved in CE. The syntrophic cooperation between Petrimonas mucosa FDU058 and Methanofollis sp. FDU007 might play important role in increased caproate production at 1 g/L phenol, and their adaption to phenol could be due to the presence of genes relating with active efflux system and refolding of proteins.
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http://dx.doi.org/10.1016/j.watres.2021.117601DOI Listing
October 2021

Modification of hydrochar increased the capacity to promote anaerobic digestion.

Bioresour Technol 2021 Dec 30;341:125856. Epub 2021 Aug 30.

Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3), Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China; Shanghai Technical Service Platform for Pollution Control and Resource Utilization of Organic Wastes, Shanghai 200438, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address:

Hydrochar has been demonstrated to increase methane production rate during anaerobic digestion (AD) of organic wastes/wastewater by facilitating direct interspecies electron transfer (DIET). The present study compared the hydrochars prepared at different conditions (260 °C-1 h, 260 °C-8 h, 320 °C-1 h and 320 °C-8 h) on AD of glucose. Hydrochar prepared at lower temperature and residence time (260 °C-1 h) resulted in the highest methane production rate, which was 237% higher of control experiment without hydrochar. Modification of hydrochar (260 °C-1 h) by ball-milling further increased the capacity to increase methane production rate. Hydrothermal liquefaction (HTL) conditions affected the surface oxygen-containing functional groups that related with DIET, and hydrochar (260 °C-1 h) had higher peaks relating with C-O and O-H functional groups. Ball-milling enhanced the formation of such groups. Microbial analysis showed hydrochar (260 °C-1 h) by ball-milling resulted in the formation of different microbial communities as compared with control experiments, and Azospira and Methanosarcina were enriched, which might be involved in DIET.
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http://dx.doi.org/10.1016/j.biortech.2021.125856DOI Listing
December 2021

Light dominates the diurnal emissions of herbivore-induced volatiles in wild tobacco.

BMC Plant Biol 2021 Aug 30;21(1):401. Epub 2021 Aug 30.

Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, 07745, Jena, Germany.

Background: Timing is everything when it comes to the fitness outcome of a plant's ecological interactions, and accurate timing is particularly relevant for interactions with herbivores or mutualists that are based on ephemeral emissions of volatile organic compounds. Previous studies of the wild tobacco N. attenuata have found associations between the diurnal timing of volatile emissions, and daytime predation of herbivores by their natural enemies.

Results: Here, we investigated the role of light in regulating two biosynthetic groups of volatiles, terpenoids and green leaf volatiles (GLVs), which dominate the herbivore-induced bouquet of N. attenuata. Light deprivation strongly suppressed terpenoid emissions while enhancing GLV emissions, albeit with a time lag. Silencing the expression of photoreceptor genes did not alter terpenoid emission rhythms, but silencing expression of the phytochrome gene, NaPhyB1, disordered the emission of the GLV (Z)-3-hexenyl acetate. External abscisic acid (ABA) treatments increased stomatal resistance, but did not truncate the emission of terpenoid volatiles (recovered in the headspace). However, ABA treatment enhanced GLV emissions and leaf internal pools (recovered from tissue), and reduced internal linalool pools. In contrast to the pattern of diurnal terpenoid emissions and nocturnal GLV emissions, transcripts of herbivore-induced plant volatile (HIPV) biosynthetic genes peaked during the day. The promotor regions of these genes were populated with various cis-acting regulatory elements involved in light-, stress-, phytohormone- and circadian regulation.

Conclusions: This research provides insights into the complexity of the mechanisms involved in the regulation of HIPV bouquets, a mechanistic complexity which rivals the functional complexity of HIPVs, which includes repelling herbivores, calling for body guards, and attracting pollinators.
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http://dx.doi.org/10.1186/s12870-021-03179-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8404343PMC
August 2021

Elucidating the Macroeconomic Determinants of Undernourishment in South Asian Countries: Building the Framework for Action.

Front Public Health 2021 12;9:696789. Epub 2021 Aug 12.

College of Economics and Management, Nanjing Agricultural University, Nanjing, China.

Undernourishment is a big challenge for humanity across the world. Considering the significance of reducing undernourishment, the current study focuses on exploring the macroeconomic determinants of undernourishment in the South Asian panel. The study employed econometric models that are more robust to underpin cross-sectional dependency and heterogeneity in a panel data set. The overall findings reveal that an increase in food production increases undernourishment and infer that food availability at the national level is insufficient to reduce undernourishment unless poor people also had economic and physical access to food. In the case of economic growth and governance, the results are negatively significant in some countries. The results infer that GDP and quality of governance are nuanced in declining the rate of undernourishment in some countries, while in other countries where the results are found insignificant, the government should seek other interventions to curtail the prevalence of undernourishment. Unexpectedly, an increase in food prices lessens the undernourishment in developing countries that reflect that food prices might transform the dietary patterns of poor people from nutrient-rich foods to nutrient-poor staples, thus lead to undernourishment reduction but trigger overweight and obesity alongside. In conclusion, the results depict that policymakers should devise strategies keeping in view fundamental aspects of the country to reduce undernourishment.
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http://dx.doi.org/10.3389/fpubh.2021.696789DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8397478PMC
August 2021

Structure, Luminescent Sensing and Proton Conduction of a Boiling-Water-Stable Zn(II) Metal-Organic Framework.

Molecules 2021 Aug 20;26(16). Epub 2021 Aug 20.

School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China.

A novel Zn(II) metal-organic framework [ZnO(CHFOS)], namely , has been constructed from a fluoro- and thiophenethio-functionalized ligand 2,2',5,5'-tetrafluoro-3,3',6,6'-tetrakis(2-thiophenethio)-4,4'-biphenyl dicarboxylic acid (). shows a broad green emission around 520 nm in solid state luminescence, with a Commission International De L'Eclairage (CIE) coordinate at x = 0.264, y = 0.403. Since d-configured Zn(II) is electrochemically inert, its photoluminescence is likely ascribed to ligand-based luminescence which originates from the well-conjugated system of phenyl and thiophenethio moieties. Its luminescent intensities diminish to different extents when exposed to various metal ions, indicating its potential as an optical sensor for detecting metal ion species. Furthermore, and its NHBr-loaded composite, , were used for proton conduction measurements in different relative humidity (RH) levels and temperatures. Original shows a low proton conductivity of 9.47 × 10 S cm while shows a more than 25,000-fold enhanced value of 2.38 × 10 S cm at 40 °C and 90% RH. Both of the proton transport processes in and belong to the Grotthuss mechanism with = 0.40 and 0.32 eV, respectively.
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http://dx.doi.org/10.3390/molecules26165044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8401761PMC
August 2021
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