Publications by authors named "Jun Guo"

1,327 Publications

  • Page 1 of 1

Real time and online aerosol identification based on deep learning of multi-angle synchronous polarization scattering indexes.

Opt Express 2021 Jun;29(12):18540-18564

In this study, we employ our developed instrument to obtain high-throughput multi-angle single-particle polarization scattering signals. Based on experimental results of a variety of samples with different chemical composition, particle size, morphology, and microstructure, we trained a deep convolutional network to identify the polarization signal characteristics during aerosol scattering processes, and then investigate the feasibility of multi-dimensional polarization characterization applied in the online and real-time fine and accurate aerosol recognition. Our model shows a high classification accuracy rate (>98%) and can achieve aerosol recognition at a very low proportion (<0.1%), and shows well generalization ability in the test set and the sample types not included in the training set. The above results indicate that that the time series pulses from multi-angle polarization scattering contain enough information related with microscopic characteristics of an individual particle, and the deep learning model shows its capability to extract features from these synchronous multi-dimensional polarization signals. Our investigations confirm a good prospect of aerosol attribute retrieval and identifying and classifying individual aerosols one by one by the combination of multi-dimensional polarization scattering indexes with deep learning method.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1364/OE.426501DOI Listing
June 2021

Immunotherapy in Acral and Mucosal Melanoma: Current Status and Future Directions.

Front Immunol 2021 4;12:680407. Epub 2021 Jun 4.

Department of Melanoma, Peking University Cancer Hospital and Institute, Beijing, China.

Acral and mucosal melanomas are extremely rare in Caucasians; however, they are the predominant melanoma subtypes in Asians and other non-Caucasian populations. Acral and mucosal melanomas share many clinicopathological features, including aggressive phenotypes, similar genetic landscapes, and grim prognoses. In spite of advances in melanoma management, patients with acral and mucosal melanomas show limited benefit from current therapies. The rarity of these subtypes of melanoma is a significant factor contributing to the poor understanding of these pathological subtypes and the lack of effective interventions. Furthermore, the mechanisms contributing to disparities between different types of melanoma remain largely unclear. Herein, we comprehensively review current knowledge on the clinicopathological characteristics and mutational landscapes of acral and mucosal melanomas, as well as providing an overview of current therapies for patients with these aggressive melanoma subtypes, focusing on available immunotherapeutic interventions. We also discuss pathological differences between different melanoma subtypes and summarize current knowledge on melanoma disparities between Asians and Caucasians. Finally, we discuss emerging immunotherapeutic strategies for the treatment of acral and mucosal melanomas, focusing on combination therapies with immune checkpoint inhibitors. Unraveling the unique features of acral and mucosal melanomas is key for their early diagnosis and for the development of effective therapies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fimmu.2021.680407DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212860PMC
June 2021

Relations between left atrial appendage contrast retention and thromboembolic risk in patients with atrial fibrillation.

J Thromb Thrombolysis 2021 Jun 15. Epub 2021 Jun 15.

Department of Cardiology, the Sixth Medical Centre, Chinese PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing, 100853, China.

Left atrial appendage (LAA), a blind pouch, accounts for more than 90% of the source of cardiac thrombus formation. Contrast retention (CR) in the LAA has been frequently observed during left atrial appendage occlusion (LAAO) procedures, especially in patients with stroke history. This study was designed to assess the relations between LAA contrast retention and thrombogenesis risk of the LAA in patients with non-valvular atrial fibrillation. A total of 132 consecutive patients who underwent LAAO were enrolled. The data collected from computed tomography (CT), transthoracic echocardiography (TTE), transesophageal echocardiography (TEE) and blood samples were analyzed. Univariate and multivariate logistic regression models were constructed to assess the association between CR, left atrial appendage thrombus (LAAT) and other factors. Contrast retention was observed in 33 patients, accounting for 25% of the population. Compared to the non-CR group, patients in the CR group had a larger left atrium anteroposterior diameter (49.64 ± 11.57 vs. 42.42 ± 7.04, P = 0.002), higher CHADS (3.88 ± 0.99 vs. 2.97 ± 1.35, P = 0.001) and CHADS-VASc scores (5.79 ± 1.14 vs. 4.89 ± 1.56, P = 0.003), a higher rate of prior stroke (90.9% vs. 66.7%, P = 0.007), more LAA lobes (3.13 ± 1.18 vs. 2.64 ± 1.12, P = 0.038), and a higher prevalence of LAAT (63.6% vs. 13.1%, P < 0.001). After having adjusted the logistic model, only contrast retention, LAA cauliflower morphology and left ventricular ejection fraction (LVEF) were independently associated with LAAT. Patients with LAA contrast retention have a higher risk of left atrial appendage thrombosis. Contrast retention may be a cardiac factor strongly associated with cardiogenic stroke.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s11239-021-02490-8DOI Listing
June 2021

Silencing of transcription factor E93 inhibits adult morphogenesis and disrupts cuticle, wing and ovary development in Locusta migratoria.

Insect Sci 2021 Jun 11. Epub 2021 Jun 11.

Research Institute of Applied Biology, Shanxi University, Taiyuan, China.

Ecdysone-induced protein 93F (E93) plays important roles during the metamorphosis process in insects. In this study, a cDNA of the LmE93 gene was identified from the transcriptome of Locusta migratoria, which consists of the 3378-nucleotide open-reading frame (ORF) and encodes 1125 amino acids with helix-turn-helix (HTH) motifs. Reverse transcription quantitative polymerase chain reaction analysis revealed that LmE93 was highest expressed in ovary. The LmE93 expression level was markedly low from the 3rd to 4th instar nymphs, and greatly increased in 1-day-old 5th instar nymphs with a peak on middle nymphal days, then declined in the late nymphal days. Moreover, injected dsLmE93 into 4th and 5th instar nymphs greatly reduced LmE93 transcripts, respectively, and prevented the process of metamorphosis, causing supernumerary nymphal stages. Hematoxylin-eosin staining of the integument showed that the apolysis occurred in advance in 4th instar nymphs, and old cuticle degradation was decreased in dsLmE93-injected locusts of 5th instar nymphs. Smaller and no fully developed wings with reduced columns between the anterior and posterior regions were found in N6 and N7 supernumerary nymphs. In addition, the development of the ovary in dsLmE93-injected locusts was severely blocked, the yolk was almost not formed and there was no development of ovarioles. The results indicated that LmE93 play key roles in the metamorphosis, cuticle, wing and ovarian development of locusts.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1111/1744-7917.12924DOI Listing
June 2021

Shape-Dependent Linear Dichroism Spectra of Colloidal Semiconductor Nanocrystals.

Langmuir 2021 Jun 11;37(24):7611-7616. Epub 2021 Jun 11.

Engineering Research Center of Clinical Functional Materials and Diagnosis &Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325000, People's Republic of China.

Semiconductor nanocrystals are normally dispersed in the solvent for property studies as well as practical applications. However, rare attention has been paid to their orientation status in the colloidal solution. Herein, with the help of linear dichroism (LD) spectroscopy, we demonstrate that isotropic NCs of high symmetry (i.e., quantum dots, QDs) and anisotropic NCs (e.g., quantum rods, QRs and nanoplates, NPLs) but under diluted concentration are randomly dispersed without any preferential orientation. Meanwhile, anisotropic NCs under a high concentration can behave with some net orientation along a certain direction. For example, CdSe quantum rods (QRs) and nanoplatelets (NPLs) both show an obviously preferred orientation along the vertical direction in solution when their solution absorbances increase to certain values. An in-depth analysis of QRs' LD spectrum shows that the first excitonic transition of QRs is strongly quantumly confined while its higher-energy excitonic transitions are weakly quantumly confined. In contrast, the NPLs' LD spectrum indicates that their excitonic transitions are isotropic in the spatial space. This work provides a new viewpoint of the real status of anisotropic semiconductor NCs in solution.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.langmuir.1c01155DOI Listing
June 2021

The composition of bacteria in gut and beebread of stingless bees (Apidae: Meliponini) from tropics Yunnan, China.

Antonie Van Leeuwenhoek 2021 Jun 10. Epub 2021 Jun 10.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.

Stingless bees are the main pollinators in tropical and subtropical regions. However, there are only a few studies on the structure and composition of bacteria in the gut and beebread of stingless bees, especially in China. To address this shortage of information, we characterized the microbiota of three common species of stingless bees (Lepidotrigona terminata, Lepidotrigona ventralis and Tetragonula pagdeni) and beebread samples of T. pagdeni. The results showed that the gut of stingless bees contained a set of dominant bacteria, including Acetobacter-like, Snodgrassella, Lactobacillus, Psychrobacter, Pseudomonas, Bifidobacterium and other species. The gut microbiota structures of the three stingless bees were different, and the abundances of bacterial species in the gut varied between communities of the same bee species. The reasons for this are manifold and may include food preference, age and genetic differences. In addition, the abundances of Lactobacillus, Carnimonas, Escherichia-Shigella, Acinetobacter and other species were high in the beebread of stingless bees. In conclusion, our findings reveal the bacteria composition and structure of the gut and beebread of stingless bees in China and deepen our understanding of the dominant bacteria of the gut and beebread of stingless bees.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10482-021-01602-xDOI Listing
June 2021

Risk Models for Advanced Melanoma Patients Under Anti-PD-1 Monotherapy- Analyses of Pooled Data From Two Clinical Trials.

Front Oncol 2021 20;11:639085. Epub 2021 May 20.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education, Beijing), Department of Melanoma, Peking University Cancer Hospital and Institute, Beijing, China.

The best response and survival outcomes between advanced melanoma patients treated with the anti-PD-1 monotherapy vary greatly, rendering a risk model in need to optimally stratify patients based on their likelihood to benefit from the said treatment. We performed an analysis of 89 advanced melanoma patients treated with the anti-PD-1 monotherapy from two prospective clinical trials at the Peking University Cancer Hospital from April 2016 to May 2018. Clinicodemographical characteristics, baseline and early-on-treatment (median 0.6 months after anti-PD-1 monotherapy initiation) routine laboratory variables, including complete blood count and general chemistry, and best response/survival data were extracted and analyzed in both univariate and multivariate logistic and Cox proportional hazard models. After three rounds of screening, risk factors associated with a poorer PFS included a high pre-treatment neutrophil, derived neutrophil-lymphocyte ratio (dNLR), low pre-treatment hemoglobin, and low early-on-/pre-treatment fold change of eosinophil; those with a poorer OS included a high pre-treatment neutrophil, eosinophil, PLT, early-on/pre-treatment fold change of LDH and neutrophil; and those with a poorer best response included a high pre-treatment NLR and early-on-/pre-treatment LDH fold change. Risk models (scale: low, median-low, median high, and high risk) were established based on these risk factors as dichotomous variables and M stage (with vs. without distant metastasis) for PFS (HR 1.976, 95% CI, 1.507-2.592, < 0.001), OS (HR 2.348, 95% CI, 1.688-3.266), and non-responder (OR 3.586, 95% CI, 1.668-7.713, = 0.001), respectively. For patients with low, median-low, median-high, and high risks of developing disease progression (PD), six-month PFS rates were 64.3% (95% CI, 43.5-95.0%), 37.5% (95% CI, 22.4-62.9%), 9.1% (95% CI, 3.1-26.7%), and 0%, respectively. For patients with OS risks of low, median-low, median-high, and high, OS rates at 12 months were 82.5% (95% CI, 63.1-100%), 76.6% (95% CI, 58.4-100%), 42.1% (95% CI, 26.3-67.3%), and 23.9% (95% CI, 11.1-51.3%), respectively. For patients with risks of low, median-low, median-high, and high of being a non-responder, objective response rates were 50.0% (95% CI, 15.7-84.3%), 27.8% (95% CI, 9.7-53.5%), 10.3% (95% CI, 2.9-24.2%), and 0%, respectively. A risk scoring model based on the clinicodemographical characteristics and easily obtainable routinely tested laboratory biomarkers may facilitate the best response and survival outcome prediction and personalized therapeutic decision making for the anti-PD-1 monotherapy treated advanced melanoma patients in Asia.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fonc.2021.639085DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8174451PMC
May 2021

Dirichlet Process Mixture of Generalized Inverted Dirichlet Distributions for Positive Vector Data With Extended Variational Inference.

IEEE Trans Neural Netw Learn Syst 2021 Jun 4;PP. Epub 2021 Jun 4.

A Bayesian nonparametric approach for estimation of a Dirichlet process (DP) mixture of generalized inverted Dirichlet distributions [i.e., an infinite generalized inverted Dirichlet mixture model (InGIDMM)] has been proposed. The generalized inverted Dirichlet distribution has been proven to be efficient in modeling the vectors that contain only positive elements. Under the classical variational inference (VI) framework, the key challenge in the Bayesian estimation of InGIDMM is that the expectation of the joint distribution of data and variables cannot be explicitly calculated. Therefore, numerical methods are usually applied to simulate the optimal posterior distributions. With the recently proposed extended VI (EVI) framework, we introduce lower bound approximations to the original variational objective function in the VI framework such that an analytically tractable solution can be derived. Hence, the problem in numerical simulation has been overcome. By applying the DP mixture technique, an InGIDMM can automatically determine the number of mixture components from the observed data. Moreover, the DP mixture model with an infinite number of mixture components also avoids the problems of underfitting and overfitting. The performance of the proposed approach is demonstrated with both synthesized data and real-life data applications.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TNNLS.2021.3072209DOI Listing
June 2021

Genome-Wide Analysis of the DUF4228 Family in Soybean and Functional Identification of in Response to Drought and Salt Stresses.

Front Plant Sci 2021 17;12:628299. Epub 2021 May 17.

College of Life Sciences/College of Agronomy, Jilin Agricultural University, Changchun, China.

Domain of unknown function 4228 (DUF4228) proteins are a class of proteins widely found in plants, playing an important role in response to abiotic stresses. However, studies on the DUF4228 family in soybean ( L.) are sparse. In this study, we identified a total of 81 genes in soybean genome, named systematically based on their chromosome distributions. Results showed that these genes were unevenly distributed on the 20 chromosomes of soybean. The predicted soybean DUF4228 proteins were identified in three groups (Groups I-III) based on a maximum likelihood phylogenetic tree. Genetic structure analysis showed that most of the genes contained no introns. Expression profiling showed that genes were widely expressed in different organs and tissues in soybean. RNA-seq data were used to characterize the expression profiles of genes under the treatments of drought and salt stresses, with nine genes showing significant up-regulation under both drought and salt stress further functionally verified by promoter (-acting elements) analysis and quantitative real-time PCR (qRT-PCR). Due to its upregulation under drought and salt stresses based on both RNA-seq and qRT-PCR analyses, was selected for further functional analysis in transgenic plants. Under drought stress, the degree of leaf curling and wilting of the -overexpressing (-OE) line was lower than that of the empty vector (EV) line. -OE lines also showed increased proline content, relative water content (RWC), and chlorophyll content, and decreased contents of malondialdehyde (MDA), HO, and O. Under salt stress, the changes in phenotypic and physiological indicators of transgenic plants were the same as those under drought stress. In addition, overexpression of the gene promoted the expression of marker genes under both drought and salt stresses. Taken together, the results indicated that genes play important roles in response to abiotic stresses in soybean.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpls.2021.628299DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166234PMC
May 2021

A new nomogram and risk classification system for predicting survival in small cell lung cancer patients diagnosed with brain metastasis: a large population-based study.

BMC Cancer 2021 May 29;21(1):640. Epub 2021 May 29.

Department of Internal Medicine-Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, 250117, Shandong, China.

Background: The prognosis of patients with small cell lung cancer (SCLC) is poor, most of them are in the extensive stage at the time of diagnosis, and are prone to brain metastasis. In this study, we established a nomogram combined with some clinical parameters to predict the survival of SCLC patients with brain metastasis.

Methods: The 3522 eligible patients selected from the SEER database between 2010 and 2015 were randomly divided into training cohort and validation cohort. Univariate and multivariate Cox regression analysis were used to evaluate the ability of each parameter to predict OS. The regression coefficients obtained in multivariate analysis were visualized in the form of nomogram, thus a new nomogram and risk classification system were established. The calibration curves were used to verify the model. And ROC curves were used to evaluate the discrimination ability of the newly constructed nomogram. Survival curves were made by Kaplan-Meier method and compared by Log rank test.

Results: Univariate and multivariate analysis showed that age, race, sex, T stage, N stage and marital status were independent prognostic factors and were included in the predictive model. The calibration curves showed that the predicted value of the 1- and 3-year survival rate by the nomogram was in good agreement with the actual observed value of the 1- and 3-year survival rate. And, the ROC curves implied the good discrimination ability of the predictive model. In addition, the results showed that in the total cohort, training cohort, and validation cohort, the prognosis of the low-risk group was better than that of the high-risk group.

Conclusions: We established a nomogram and a corresponding risk classification system to predict OS in SCLC patients with brain metastasis. This model could help clinicians make clinical decisions and stratify treatment for patients.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12885-021-08384-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8164795PMC
May 2021

Advanced Dropout: A Model-free Methodology for Bayesian Dropout Optimization.

IEEE Trans Pattern Anal Mach Intell 2021 May 24;PP. Epub 2021 May 24.

Due to lack of data, overfitting ubiquitously exists in real-world applications of deep neural networks (DNNs). We propose advanced dropout, a model-free methodology, to mitigate overfitting and improve the performance of DNNs. The advanced dropout technique applies a model-free and easily implemented distribution with parametric prior, and adaptively adjusts dropout rate. Specifically, the distribution parameters are optimized by stochastic gradient variational Bayes in order to carry out an end-to-end training. We evaluate the effectiveness of the advanced dropout against nine dropout techniques on seven computer vision datasets (five small-scale datasets and two large-scale datasets) with various base models. The advanced dropout outperforms all the referred techniques on all the datasets.We further compare the effectiveness ratios and find that advanced dropout achieves the highest one on most cases. Next, we conduct a set of analysis of dropout rate characteristics, including convergence of the adaptive dropout rate, the learned distributions of dropout masks, and a comparison with dropout rate generation without an explicit distribution. In addition, the ability of overfitting prevention is evaluated and confirmed. Finally, we extend the application of the advanced dropout to uncertainty inference, network pruning, text classification, and regression. The proposed advanced dropout is also superior to the corresponding referred methods.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1109/TPAMI.2021.3083089DOI Listing
May 2021

First Report of Leaf Spot Caused by Botrytis cinerea on Cardamine hupingshanensis in China.

Plant Dis 2021 May 24. Epub 2021 May 24.

Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha City, Hunan Province, Changsha, China;

Cardamine hupingshanensis is a selenium (Se) and cadmium (Cd) hyperaccumulator plant distributed in wetlands along the Wuling Mountains of China (Zhou et al. 2018). In March of 2020, a disease with symptoms similar to gray mold was observed on leaves of C. hupingshanensis in a nursery located in Changsha, Hunan Province, China. Almost 40% of the C. hupingshanensis (200 plants) were infected. Initially, small spots were scattered across the leaf surface or margin. As disease progressed, small spots enlarged to dark brown lesions, with green-gray, conidia containing mold layer under humid conditions. Small leaf pieces were cut from the lesion margins and were sterilized with 70% ethanol for 10 s, 2% NaOCl for 2 min, rinsed with sterilized distilled water for three times, and then placed on potato dextrose agar (PDA) medium at 22°C in the dark. Seven similar colonies were consistently isolated from seven samples and further purified by single-spore isolation. Strains cultured on PDA were initially white, forming gray-white aerial mycelia, then turned gray and produced sclerotia after incubation for 2 weeks, which were brown to blackish, irregular, 0.8 to 3.0 × 1.2 to 3.5 mm (n=50). Conidia were unicellular, globose or oval, colourless, 7.5 to 12.0 × 5.5 to 8.3 μm (n=50). Conidiophores arose singly or in group, straight or flexuous, septate, brownish to light brown, with enlarged basal cells, 12.5 to 22.1 × 120.7 to 310.3 μm. Based on their morphological characteristics in culture, the isolates were putatively identified as Botrytis cinerea (Ellis 1971). Genomic DNA of four representative isolates, HNSMJ-1 to HNSMJ-4, were extracted by CTAB method. The internal transcribed spacer region (ITS), glyceraldehyde-3-phosphate dehydrogenase gene (G3PDH), heat-shock protein 60 gene (HSP60), ATP-dependent RNA helicaseDBP7 gene (MS547) and DNA-dependent RNA polymerase subunit II gene (RPB2) were amplified and sequenced using the primers described previously (Aktaruzzaman et al. 2018) (MW820311, MW831620, MW831628, MW831623 and MW831629 for HNSMJ-1; MW314722, MW316616, MW316617, MW316618 and MW316619 for HNSMJ-2; MW820519, MW831621, MW831627, MW831624 and MW831631 for HNSMJ-3; MW820601, MW831622, MW831626, MW831625 and MW831630 for HNSMJ-4). BLAST searches showed 99.43 to 99.90% identity to the corresponding sequences of B. cinerea strains, such as HJ-5 (MF426032.1, MN448500.1, MK791187.1, MH727700.1 and KX867998.1). A combined phylogenetic tree using the ITS, G3PDH, HSP60 and RPB2 sequences was constructed by neighbor-joining method in MEGA 6. It revealed that HNSMJ-1 to HNSMJ-4 clustered in the B. cinerea clade. Pathogenicity tests were performed on healthy pot-grown C. hupingshanensis plants. Leaves were surface-sterilized and sprayed with conidial suspension (106 conidia/ mL), with sterile water served as controls. All plants were kept in growth chamber with 85% humidity at 25℃ following a 16 h day-8 h night cycle. The experiment was repeated twice, with each three replications. After 4 to 7 days, symptoms similar to those observed in the field developed on the inoculated leaves, whereas controls remained healthy. The pathogen was reisolated from symptomatic tissues and identified using molecular methods, confirming Koch's postulates. B. cinerea has already been reported from China on C. lyrate (Zhang 2006), a different species of C. hupingshanensis. To the best of our knowledge, this is the first report of B. cinerea causing gray mold on C. hupingshanensis in China and worldwide. Based on the widespread damage in the nursery, appropriate control strategies should be adopted. This study provides a basis for studying the epidemic and management of the disease.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1094/PDIS-04-21-0698-PDNDOI Listing
May 2021

Intravenous immunoglobulin treatment for patients with severe COVID-19: a retrospective multi-center study.

Clin Microbiol Infect 2021 May 18. Epub 2021 May 18.

Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No.197 Ruijin 2nd Road, Shanghai 200025, China. Electronic address:

Objectives: Intravenous immunoglobulin (IVIG) is commonly used to treat severe COVID-19, although the clinical outcome of such treatment remains unclear. This study evaluated the effectiveness of IVIG treatment in severe COVID-19 patients.

Methods: This retrospective multicenter study evaluated 28-day mortality in severe COVID-19 patients with or without IVIG treatment. Each patient treated with IVIG was matched with one untreated patient. Logistic regression and inverse probability weighting(IPW)was used to control confounding factors.

Results: The study included 850 patients (421 IVIG treated patients and 429 non-IVIG treated patients). After matching, 406 patients per group remained. No significant difference in 28-day mortality was observed after IPW analysis (ATE = 0.008, 95% CI -0.081-0.097, p = 0.863). There were no significant differences between the IVIG group and non-IVIG group for acute respiratory distress syndrome, diffuse intravascular coagulation, myocardial injury, acute hepatic injury, shock, acute kidney injury, non-invasive mechanical ventilation, invasive mechanical ventilation, continuous renal replacement therapy, and extracorporeal membrane oxygenation except for prone position ventilation (ATE = -0.022, 95% CI -0.041- -0.002, p = 0.028).

Conclusions: IVIG treatment was not associated with significant changes in 28-day mortality in severe COVID-19 patients. The effectiveness of IVIG in treating patients with severe COVID-19 needs to be further investigated through future studies.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cmi.2021.05.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131555PMC
May 2021

Iron- and Cobalt-Catalyzed Asymmetric Hydrofunctionalization of Alkenes and Alkynes.

Acc Chem Res 2021 06 19;54(11):2701-2716. Epub 2021 May 19.

Department of Chemistry, Zhejiang University, Hangzhou 310058, China.

Transition metal catalyzed asymmetric hydrofunctionalization of readily available unsaturated hydrocarbons presents one of the most straightforward and atom-economic protocols to access valuable optically active products. For decades, noble transition metal catalysts have laid the cornerstone in this field, on account of their superior reactivity and selectivity. In recent years, from an economical and sustainable standpoint, first-row, earth-abundant transition metals have received considerable attention, due to their high natural reserves, affordable costs, and low toxicity. Meanwhile, the earth-abundant metal catalyzed hydrofunctionalization reactions have also gained much interest and been investigated gradually. However, since chiral ligand libraries for earth-abundant transition-metal catalysis are limited to date, the development of highly enantioselective versions remains a significant challenge.This Account summarizes our recent efforts in developing suitable chiral ligands for iron and cobalt catalysts and their applications in the highly enantioselective hydrofunctionalization reactions (hydroboration and hydrosilylation) of alkenes and alkynes. In ligand design, we envisioned that chiral unsymmetric -tridentate (UNT) ligand scaffolds could promote these enantioselective transformations with earth-abundant metals. Therefore, several types of chiral UNT ligands were designed and prepared in our laboratory, utilizing readily available natural amino acids as chiral sources. In the very beginning, chiral oxazoline iminopyridine (OIP) ligands were proposed and investigated through the rational combination of nitrogen-containing ligand scaffolds. After a systematic survey of the ligand effects, the imine moiety in the rigid OIP ligands was replaced by a conformationally more flexible amine unit, leading to the construction of reactive oxazoline aminoisopropylpyridine (OAP) ligands. Subsequently, imidazoline iminopyridine (IIP) and thiazoline iminopyridine (TIP) ligands were prepared by altering the oxygen atom of oxazoline with nitrogen and sulfur linkers, respectively. To further expand the chiral ligand library, other tridentate ligands containing a twisted pincer, anionic, and nonrigid backbone were also designed and synthesized, including iminophenyl oxazolinyl phenylamine (IPOPA) and imidazoline phenyl picolinamide (ImPPA). The efficacy of these chiral UNT ligands for asymmetric induction in iron and cobalt catalysis has been demonstrated through asymmetric hydrofunctionalization of alkenes and asymmetric sequential hydrofunctionalization of alkynes, which exhibit excellent reactivity as well as high chemo-, regio-, and stereoselectivity with broad functional group tolerance. Notably, highly regio- and enantioselective hydrofunctionalization of challenging substrates, such as 1,1-disubstituted aryl alkenes and terminal aliphatic alkenes, was also achieved. Furthermore, the development of asymmetric sequential isomerization/hydroboration of internal alkenes and sequential hydrofunctionalization of alkynes further demonstrates the synthetic power of these catalytic systems. The chiral enantioenriched products obtained by these methodologies could be potentially utilized in organic synthesis, medicinal chemistry, and materials science. We believe that our continuous efforts in this field would be beneficial to the development of asymmetric earth-abundant metal catalysis.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.accounts.1c00212DOI Listing
June 2021

High-Stability Flexible X-ray Detectors Based on Lead-Free Halide Perovskite CsTeI Films.

ACS Appl Mater Interfaces 2021 May 17;13(20):23928-23935. Epub 2021 May 17.

State Key Laboratory of Solidification Processing, Northwestern Polytechnical University, Xi'an 710072, China.

Normal flat panel X-ray detectors are confined in imaging of curved surfaces and three-dimensional objects. Except that, their rigid panels provide uncomfortable user experience in medical diagnosis. Here, we report a flexible X-ray detector fabricated by the combination of a lead-free CsTeI perovskite film and a polyimide (PI) substrate. High-quality CsTeI polycrystalline films are prepared by a low-temperature electrospraying method. The resistivity even remained at the level of 10 Ω·cm after 100 cycles of bending tests with a low bending radius of 10 mm. The resulting flexible CsTeI detectors exhibit better response stability than those based on rigid SnO:F glass (FTO), which is attributed to the superior crystallization of films and the growth stress relief of flexible substrates. Furthermore, an X-ray sensitivity of 76.27 μC·Gy·cm and a detection limit of 0.17 μGy·s are achieved. A series of distortion-free clear X-ray images are obtained for objects with different materials and densities. These findings provide insights into flexible X-ray detectors based on perovskite films and motivate research in wearable X-ray detectors for medical radiography and dose monitoring.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c04252DOI Listing
May 2021

Circ-EGFR Functions as an Inhibitory Factor in the Malignant Progression of Glioma by Regulating the miR-183-5p/TUSC2 Axis.

Cell Mol Neurobiol 2021 May 15. Epub 2021 May 15.

Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, No.127, Changle West Road, Xincheng District, Xi'an, 710032, China.

Circular RNAs (circRNAs) have pivotal functions in regulating diverse biological processes of human tumors, including glioma. Herein, a novel circRNA epidermal growth factor receptor (circ-EGFR, hsa_circ_0080223) was researched in glioma. The molecular expression levels were analyzed via quantitative real-time polymerase chain reaction (qRT-PCR). Cell Counting Kit-8 (CCK-8) and colony formation assays were conducted to assess cell proliferation. Apoptosis was analyzed using flow cytometry. Cell migration and invasion were examined via transwell assay. Interaction relations between targets were verified using dual-luciferase reporter assay. Tumor Suppressor Candidate 2 (TUSC2) protein expression was examined by Western blot. In vivo experiment was performed by establishing xenograft model in mice. The qRT-PCR showed the downregulation of circ-EGFR and TUSC2 but the upregulation of microRNA-183-5p (miR-183-5p) in glioma samples. In vitro assays revealed that circ-EGFR overexpression induced the repression of cell proliferation, migration, and invasion but the promotion of apoptosis. Circ-EGFR was identified as a sponge of miR-183-5p and circ-EGFR-mediated glioma progression inhibition was abolished by miR-183-5p downregulation. Additionally, miR-183-5p targeted TUSC2 and miR-183-5p inhibitor impeded the development of glioma by upregulating the expression of TUSC2. Furthermore, circ-EGFR could regulate the TUSC2 level by sponging miR-183-5p. Glioma growth in vivo was also reduced by circ-EGFR via targeting the miR-183-5p/TUSC2 axis. Altogether, our results suggested that circ-EGFR inhibited the malignant progression of glioma by regulating the levels of miR-183-5p and TUSC2. Circ-EGFR may be a useful therapeutic target to antagonize the glioma progression.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10571-021-01099-yDOI Listing
May 2021

Glibenclamide Advantage in Treating Edema After Intracerebral Hemorrhage (GATE-ICH): Study Protocol for a Multicenter Randomized, Controlled, Assessor-Blinded Trial.

Front Neurol 2021 27;12:656520. Epub 2021 Apr 27.

Department of Neurology, Xijing Hospital, Fourth Military Medical University (Air Force Medical University), Xi'an, China.

Brain edema after acute intracerebral hemorrhage (ICH) plays a critical role in the secondary injury of ICH and may heighten the potential for a poor outcome. This trial aims to explore the efficacy of small doses of oral glibenclamide in perihematomal edema (PHE) and the prognosis of patients with ICH. The GATE-ICH trial is a multicenter randomized, controlled, assessor-blinded trial. A total of 220 adult patients with acute primary ICH in 28 study centers in China will be randomized to the glibenclamide group (glibenclamide plus guideline-recommended ICH management) or the control group (guideline-recommended ICH management). Multivariate logistic regression will be used to analyze the relationship between the treatments and primary outcome. The primary efficacy outcome is the proportion of poor functional outcomes (modified Rankin Scale ≥3) at 90 days after enrollment. The secondary efficacy outcomes include changes in the volume of ICH and PHE between the baseline and follow-up computed tomography scans as well as the clinical scores between the baseline and follow-up assessments. The GATE-ICH trial will assess the effects of small doses of oral glibenclamide in reducing the PHE after ICH and improving the 90-day prognosis of patients. www.clinicaltrials.gov., NCT03741530. Registered on November 8, 2018. Protocol version: May 6, 2019, Version 5. Recruitment and follow-up of patients is currently ongoing. This trial will be end in the second quarter of 2021.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fneur.2021.656520DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110908PMC
April 2021

Impact of One Versus Two Consecutive Doses of Endothelial Cells (EPCs) and EPCs-Derived Condition Medium on Protecting Myocardium from Acute Ischemia-Reperfusion Injury in Rat.

Cell Transplant 2021 Jan-Dec;30:9636897211007049

Department of Cardiology, The First Affiliated Hospital, Jinan University, Guangzhou, China.

This study tested the impact of single dose and two doses of endothelial progenitor cells (EPCs) and EPCs-derived condition medium (CM) on protecting the left-ventricular myocardium (LVM) from acute ischemia-reperfusion (IR) injury. In vitro study showed EPCs and CM had comparably higher capacity for enhancement of angiogenesis as compared with the controls (all < .001). Adult-male SD rats ( = 36) were equally categorized into groups 1 (sham-operated control), 2 (IR+vehicle), 3 [IR+EPCs/1.2 × 10/intravenous administration at 3 h after IR procedure), 4 (IR+EPCs/1.2 × 10/at 3 h/24 h after IR), 5 (IR+CM/3.0cc/intravenous administration at 3 h after IR), 6 (IR+EPCs/3.0cc/at 3h/24 h after IR), and euthanized by day 3 after IR. The left-ventricular-ejection-fraction, protein and cellular expressions of endothelial-cell markers (CD31/vWF), small vessel number and protein expression of mitochondrial (mitochondrial-cytochrome-C) integrity were highest in group 1, lowest in group 2, significantly higher in group 4 than in groups 3/5/6 and significantly higher in groups 3/6 than in group 5 but they showed no differences in groups3/6, whereas the protein expressions of apoptotic (cleaved-caspase 3/cleaved-PARP), fibrotic (Smad3/TGF-ß), mitochondrial-damaged (cytosolic-cytochrome-C), heart-failed/pressure-overload (BNP), oxidative-stress (p47phox/NOX-1/NOX-2/oxidized protein), and autophagic (LCB3-II/LCB3-I) biomarkers and fibrotic/collagen-deposition areas exhibited an opposite pattern to endothelial-cell markers (all < .0001). The protein expressions of angiogenesis (VEGF/SDF-1α/CXCR4/HIF-1α) were lowest in group 1, highest in group 4, significantly higher in groups 3/6 than in groups 2/5, significantly higher in group 5 than in group 2, but they showed no difference between groups 3/6 (all < .0001). These results demonstrate that two consecutive doses of EPC/CM were superior to just one at protecting LVM against IR injury.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1177/09636897211007049DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8120601PMC
May 2021

Clinical significance of CCNE1 copy number gain in acral melanoma patients.

Melanoma Res 2021 May 6. Epub 2021 May 6.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Renal Cancer and Melanoma, Peking University Cancer Hospital & Institute, Beijing Department of Oncology, Henan Provincial People's Hospital: Zhengzhou University People's Hospital, Henan University People's Hospital, Zhengzhou, Henan Key laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Radiation Oncology, Peking University Cancer Hospital & Institute, Beijing, China.

Copy number variations are frequently observed in cell cycle-related genes in acral melanoma. However, the clinical significance of copy number gain of CCNE1 in acral melanoma has not been fully elucidated. In this study, 490 acral melanoma samples were examined for CCNE1 copy number using the QuantiGenePlex DNA Assay. Correlation between CCNE1 copy number and acral melanoma patients' clinicopathologic features were analyzed using Chi-squared test. The impact of CCNE1 copy number on patients' progression-free survival (PFS) and overall survival (OS) probability were analyzed using Kaplan-Meier analysis. The impact of CCNE1 copy number on patients' median PFS after receiving chemotherapy was also evaluated. The results showed that CCNE1 copy number gain was observed in 28.30% of patients, with 3.16% of patients carrying both CCNE1 copy number gain and BRAF mutation and 4.34% of patients carrying both CCNE1 copy number gain and NRAS mutation. The median PFS time for patients with CCNE1 copy number gain was shorter than that of patients without CCNE1 copy number gain (17.0 vs. 27.0 months, P = 0.002).In the cohort that received chemotherapy (n = 82), the median PFS time for patients with CCNE1 copy number gain was shorter than that of patients without CCNE1 copy number gain (4.8 vs. 7.4 months, P = 00.006). CCNE1 copy number gain was an independent prognostic marker for acral melanoma patients' PFS. Our study indicates that CCNE1 copy number gain is frequent in acral melanoma and may be a biomarker to predict acral melanoma patients' outcomes after receiving chemotherapy.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1097/CMR.0000000000000742DOI Listing
May 2021

Sub-chronic exposure to PhIP induces oxidative damage and DNA damage, and disrupts the amino acid metabolism in the colons of Wistar rats.

Food Chem Toxicol 2021 Jul 1;153:112249. Epub 2021 May 1.

Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin, 300071, China. Electronic address:

Heterocyclic amines (HCAs) are a group of mutagenic compounds produced during thermal processing of protein-rich foods. One of the most abundant HCAs, 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine (PhIP) has potential carcinogenic and mutagenic effects on human organs, especially the colon. This study aimed to explore the toxic effects of PhIP on amino acid metabolism in the colon of Wistar rats using RNA-seq and LC-MS/MS. Exposure to PhIP for 4 weeks induced oxidative damage and DNA damage in the colons, and disrupted the expression of related genes involved in tryptophan metabolism, beta(β)-alanine metabolism, valine, leucine, and isoleucine degradation, and glutathione metabolic pathways. Moreover, the levels of fecal metabolites related to amino acid metabolism were affected by PhIP. Cumulatively, these results indicate that PhIP can induce colonic oxidative injury and disorders related to amino acid metabolism, thereby providing a new theoretical basis for the study of PhIP toxicity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.fct.2021.112249DOI Listing
July 2021

Exome sequencing as the first-tier test for pediatric respiratory diseases: A single-center study.

Hum Mutat 2021 May 3. Epub 2021 May 3.

Beijing Key Laboratory for Genetics of Birth Defects, Beijing Pediatric Research Institute, MOE Key Laboratory of Major Diseases in Children, Genetics and Birth Defects Control Center, Beijing Children's Hospital, National Center for Children's Health, Capital Medical University, Beijing, China.

The high clinical and genetic heterogeneity makes it difficult to reach a confirmative diagnosis of suspected pediatric respiratory inherited diseases. Many patients with monogenic respiratory disorders could be missed without genetic testing. We performed a single-center study in Beijing Children's Hospital to demonstrate the clinical utility of exome sequencing (ES) as a first-tier test by evaluating the diagnostic yields of ES for inherited diseases with respiratory symptoms. A total of 107 patients were recruited in this study. We identified 51 pathogenic or likely pathogenic variants in 37 patients by ES (with or without copy number variants sequencing). The overall diagnostic yield was 34.6% (37/107). The most frequent disorders in our cohort were primary immunodeficiency disease (PIDs) (18/37, 48.6%) and primary ciliary dyskinesia (PCD) (9/37, 24.3%). We further reviewed the directive outcomes of genetic testing on the 37 positive cases. Our study demonstrated the effectiveness of ES as a first-tier test in China for diagnosing monogenic diseases of the respiratory system. In the era of precision medicine, ES as a first-tier test can rapidly make a molecular diagnosis and direct the intervention of the positive cases in pediatric respiratory medicine.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1002/humu.24216DOI Listing
May 2021

The Succession of the Gut Microbiota in Insects: A Dynamic Alteration of the Gut Microbiota During the Whole Life Cycle of Honey Bees ().

Front Microbiol 2021 14;12:513962. Epub 2021 Apr 14.

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.

The Asian honey bee is a valuable biological resource insect that plays an important role in the ecological environment and agricultural economy. The composition of the gut microbiota has a great influence on the health and development of the host. However, studies on the insect gut microbiota are rarely reported, especially studies on the dynamic succession of the insect gut microbiota. Therefore, this study used high-throughput sequencing technology to sequence the gut microbiota of at different developmental stages (0 days post emergence (0 dpe), 1 dpe, 3 dpe, 7 dpe, 12 dpe, 19 dpe, 25 dpe, 30 dpe, and 35 dpe). The results of this study indicated that the diversity of the gut microbiota varied significantly at different developmental stages (ACE, = 0.045; Chao1, = 0.031; Shannon, = 0.0019; Simpson, = 0.041). In addition, at the phylum and genus taxonomic levels, the dominant constituents in the gut microbiota changed significantly at different developmental stages. Our results also suggest that environmental exposure in the early stages of development has the greatest impact on the gut microbiota. The results of this study reveal the general rule of gut microbiota succession in the life cycle. This study not only deepens our understanding of the colonization pattern of the gut microbiota in workers but also provides more comprehensive information for exploring the colonization of the gut microbiota in insects and other animals.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2021.513962DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8079811PMC
April 2021

Transmission dynamics of the rubella virus circulating in China during 2010-2019: two lineage switches between genotypes 1E and 2B.

Clin Infect Dis 2021 Apr 27. Epub 2021 Apr 27.

Yunnan Provincial Center for Disease Control and Prevention, Kunming, China.

Background: To provide a better understanding of the progress on rubella control and elimination in China, a genetic analysis was conducted to examine the transmission pattern of the endemic rubella virus in China during 2010-2019.

Methods: Total 4895 strains were obtained from 29 out of the 31 provinces in mainland of China during 2010-2019. The genotyping region of the strains were amplified, determined, and assembled. Genotyping analysis and lineage division were performed by comparisons with the World Health Organization reference strains and previously reported lineage reference strains, respectively. Further phylogenetic analyses were performed to compare the genetic relationship.

Results: During 2010-2019, the domestic lineage 1E-L1 and multiple imported lineages of rubella viruses including 2B-L1, 1E-L2, and 2B-L2c were identified. Further analysis of the circulation trend of the different lineages indicated that two switches occurred among the lineages. The first shift was from lineage 1E-L1 to 2B-L1, which occurred around 2015-2016, followed by the lowest rubella incidence in 2017. The second shift was from lineage 2B-L1 to 1E-L2 and 2B-L2c, which occurred around 2018-2019, coinciding with rubella resurgence and the subsequent nationwide epidemic during 2018-2019. Insufficient genomic information worldwide made it impossible to trace the origin of the imported viruses in this study.

Conclusions: China was moving toward rubella elimination, as evidenced by the fact that previous endemic lineages were not detected. However, rubella reemerged in 2018 and 2019 due to the newly imported rubella viruses. Therefore, to realize the rubella elimination goal, joint efforts are required for all countries worldwide.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1093/cid/ciab339DOI Listing
April 2021

A general MOF-intermediated synthesis of hollow CoFe-based trimetallic phosphides composed of ultrathin nanosheets for boosting water oxidation electrocatalysis.

Nanoscale 2021 Apr 12;13(15):7279-7284. Epub 2021 Apr 12.

College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, PR China.

Engineering an electrode material for boosting reaction kinetics is highly desired for the oxygen evolution reaction (OER) in the anodic half reaction, and is still a grand challenge for energy conversion technologies. By taking inspiration from the catalytic properties of transition metal phosphides (TMPs) and metal-organic frameworks (MOFs), we herein propose a general MOF-intermediated synthesis of a series of hollow CoFeM (M = Bi, Ni, Mn, Cu, Ce, and Zn) trimetallic phosphides composed of ultrathin nanosheets as advanced electrocatalysts for the OER. A dramatic improvement of electrocatalytic performance toward the OER is observed for hollow CoFeM trimetallic phosphides compared to bimetallic CoFe phosphides. Remarkably, composition-optimized CoFeBiP hollow microspheres could deliver superior electrocatalytic performance, achieving a current density of 10 mA cm with an overpotential of only 273 mV. Mechanistic investigations reveal that the Bi and P doping effectively optimizes the electronic structure of Co and Fe by charge redistribution, which significantly lowers the adsorption energy of oxygen intermediates. Moreover, the hollow microsphere structures composed of ultrathin nanosheets also enable them to provide rich surface active sites to boost the electrocatalytic OER.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d1nr00075fDOI Listing
April 2021

Risk Factors for and Clinical Outcomes of Carbapenem-Resistant Nosocomial Infections: A Retrospective Study in a Tertiary Hospital in Beijing, China.

Infect Drug Resist 2021 13;14:1393-1401. Epub 2021 Apr 13.

School of Clinical Medicine, Tsinghua University, Beijing, 100084, People's Republic of China.

Purpose: Carbapenem-resistant (CRKP) infections have been increasingly reported worldwide. We aimed to identify the risk factors for nosocomial CRKP infections and assess the clinical outcomes.

Patients And Methods: We conducted a case-control study with data collected from January 2016 to December 2018 in China. Controls were selected at a ratio of 1:1 from patients with nosocomial carbapenem-susceptible (CSKP) infections. Risk factors for nosocomial CRKP infections and clinical outcomes were assessed with univariate and multivariate analyses.

Results: A total of one hundred forty-two patients with CRKP infections and one hundred forty-two patients with CSKP infections were enrolled in this study. Multivariate analysis showed that exposure to antibiotics within 3 months prior to admission (odds ratio OR, 2.585; 95% confidence interval [CI], 1.425-4.691; P=0.002), exposure to carbapenems (OR, 2.532; 95% CI, 1.376-4.660; P=0.003), exposure to fluoroquinolones (OR, 3.309; 95% CI, 1.326-8.257; P=0.010), and the presence of a nasogastric tube (OR, 2.796; 95% CI, 1.369-5.712; P=0.005) were independent risk factors for CRKP infections. The 30-day mortality rate in the CRKP group was 19.7%, while the in-hospital mortality rate was 28.9%. In the CRKP group, a higher creatinine level (OR, 1.009; 95% CI, 1.002-1.016; P = 0.013), being in shock at the time of a positive culture (OR, 4.454; 95% CI, 1.374-14.443; P = 0.013), and co-infection with other resistant bacteria (OR, 4.799; 95% CI, 1.229-18.740; P = 0.024) were independent predictors of in-hospital mortality in patients with CRKP infections. Kaplan-Meier curves showed that the CRKP group had a shorter survival time than the CSKP group.

Conclusion: Nosocomial CRKP infection was associated with exposure to carbapenems and fluoroquinolones within 3 months prior to hospitalization and the presence of a nasogastric tube. Patients infected with CRKP had higher 30-day and in-hospital mortality rates. A higher creatinine level, shock and co-infection with other resistant bacteria were independent predictors of in-hospital mortality in patients with CRKP infections.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.2147/IDR.S298530DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053504PMC
April 2021

Revisiting the Grain and Valence Effect of Oxide-Derived Copper on Electrocatalytic CO Reduction Using Single Crystal Cu(111) Foils.

J Phys Chem Lett 2021 Apr 19;12(16):3941-3950. Epub 2021 Apr 19.

Soochow Institute for Energy and Materials Innovations, College of Energy, Soochow University, Suzhou 215006, P. R. China.

Oxide-derived Cu (OD-Cu) has been viewed as a highly active form for catalyzing the multielectron transfer of electrochemical CO reduction, but the underlying catalytic mechanism is still controversial. In the current study, the crystalline and valency factors that influence the COR activities of OD-Cu are revisited by employing single crystal Cu(111) foils that exclude convolutions from initial morphological and crystallographic heterogeneity. We observe that the overall COR performance, especially the CH selectivity, correlates well with the initial oxidation level of the Cu(111) foil, of which the surface oxide layer is reduced into small fragments comprising rich grain boundaries and diversely orientated facets. Nonetheless, we find that the polycrystallinity and grain boundaries of OD-Cu, in this circumstance, are not the major causes of the observed activity enhancement. Instead, a transition state between the initial oxide and the finally reduced copper phases, as well as its longevity, dictates the catalytic property of OD-Cu in electrochemical CO reduction. Consequently, this work furnishes further evidence and in-depth understanding to help clarify the catalytic mechanism of OD-Cu in COR.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jpclett.1c00588DOI Listing
April 2021

Metal-organic frameworks as catalytic selectivity regulators for organic transformations.

Chem Soc Rev 2021 May 19;50(9):5366-5396. Epub 2021 Apr 19.

Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, Institute of Molecular Aggregation Science, Tianjin University, Tianjin 300072, China.

Selective organic transformations using metal-organic frameworks (MOFs) and MOF-based heterogeneous catalysts have been an intriguing but challenging research topic in both the chemistry and materials communities. Analogous to the reaction specificity achieved in enzyme pockets, MOFs are also powerful platforms for regulating the catalytic selectivity via engineering their catalytic microenvironments, such as metal node alternation, ligand functionalization, pore decoration, topology variation and others. In this review, we provide a comprehensive introduction and discussion about the role of MOFs played in regulating and even boosting the size-, shape-, chemo-, regio- and more appealing stereo-selectivity in organic transformations. We hope that it will be instructive for researchers in this field to rationally design, conveniently prepare and elaborately functionalize MOFs or MOF-based composites for the synthesis of high value-added organic chemicals with significantly improved selectivity.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0cs01538eDOI Listing
May 2021

Feasibility and Safety of the Novel Vivolight Frequency Domain Optical Coherence Tomography System: A Multicenter Study.

J Invasive Cardiol 2021 Jun 8;33(6):E412-E415. Epub 2021 Apr 8.

Department of Cardiology, The General Hospital of the People's Liberation Army, 28 Fuxing Road, Beijing (Wukesong), China.

Objective: The study sought to assess the effectiveness and safety of the novel P60 Vivolight frequency-domain optical coherence tomography (OCT) system (Shenzhen Vivolight Medical Device & Technology).

Methods: A total of 90 patients were enrolled from 3 institutions. The pullbacks were performed with both the P60 Vivolight OCT system and the Ilumien Optis OCT system (Abbott Vascular). The primary endpoint was the clear stent length (CSL). Device safety was assessed by the record of serious procedure-related or postprocedure adverse events. The secondary endpoints were the average lumen area of stent, clear image length (CIL), system stability, and imaging catheter operability.

Results: The mean relative errors of CSL were 3.30% (95% confidence interval [CI], -0.71 to 7.31) in the full analysis set (FAS) and 0.83% (95% CI, -1.79 to 3.45) in the per-protocol set (PPS). The mean relative errors of the average lumen area of stent were 2.20% (95% CI, 0.70 to 3.80) in the FAS and 1.55% (95% CI, 0.30 to 2.80) in the PPS. No difference was observed in the percentage of obtaining >24 mm of CIL (93.18% in the P60 Vivolight group vs 95.45% in the Ilumien Optis group; P=.48). There were no serious procedure-related or postprocedure adverse events.

Conclusions: The feasibility and safety of the novel Vivolight OCT system is equivalent to that of the Ilumien Optis OCT system.
View Article and Find Full Text PDF

Download full-text PDF

Source
June 2021

[Exploration of Rapid Screening Mode of Wearable Intelligent ECG Device].

Zhongguo Yi Liao Qi Xie Za Zhi 2021 Apr;45(2):228-230

Cardiocloud Medical Technology(Beijing) Co. Ltd., Beijing, 100036.

This study established a rapid ECG screening system through the application of wearable ECG equipment. The closed-loop and self-service process of ECG inspection, data collection, transmission and printing have been realized. The new rapid ECG screening system docking with HIS system in the hospital, forming a new intelligent mode of rapid ECG screening. This paper introduces the design of the intelligent mode of ECG rapid screening from the aspects of hardware, software, wearable ECG examination equipment, and briefly describes its implementation path and technical scheme. With the rapid ECG screening system, human power can be saved, the timeliness of ECG examination can be enhanced. The level of ECG diagnosis in the basic units can be improved through building a multiple medical centers which is rely on the cloud platform.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3969/j.issn.1671-7104.2021.02.022DOI Listing
April 2021

LOXL4 Abrogation Does Not Exaggerate Angiotensin II-Induced Thoracic or Abdominal Aortic Aneurysm in Mice.

Genes (Basel) 2021 Mar 31;12(4). Epub 2021 Mar 31.

Beijing Key Laboratory for Genetics of Birth Defects, Beijing Pediatric Research Institute, MOE Key Laboratory of Major Diseases in Children, Capital Medical University, Center of Rare Diseases, National Center for Children's Health, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.

It has been shown that thoracic aortic aneurysm and dissection (TAAD) could be a Mendelian trait caused by a single gene mutation. The gene mutation leads to the development of human TAAD. The gene is a member of the lysyl oxidase gene family. We identified seven variants in the gene in 219 unrelated patients with TAAD by whole-exome sequencing (WES). To further investigate whether is a candidate causative gene for human TAAD, a knockout mouse was generated, and the mutant mice were treated by subcutaneous infusion of angiotensin II. We found that abrogation of did not induce a more severe thoracic or abdominal aortic aneurysm compared with the wild-type C57BL/6J mice. Our results suggest that may not play a major role in the development of angiotensin II-induced aortic aneurysm. The functional study using this animal model system is important for the evaluation of candidate genes of TAAD identified by WES.
View Article and Find Full Text PDF

Download full-text PDF

Source
http://dx.doi.org/10.3390/genes12040513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066229PMC
March 2021