Publications by authors named "Jun Fu"

464 Publications

Hydration and Thermal Response Kinetics of a Cross-Linked Thermoresponsive Copolymer Film on a Hydrophobic PAN Substrate Coating Probed by Neutron Reflectivity.

Langmuir 2021 Jun 27;37(22):6819-6829. Epub 2021 May 27.

Technische Universität München, Physik-Department, Lehrstuhl für Funktionelle Materialien, James-Franck-Str. 1, 85748 Garching, Germany.

The hydration and thermal response kinetics of the cross-linked thermoresponsive copolymer poly((diethylene glycol monomethyl ether methacrylate)--poly(ethylene glycol) methyl ether methacrylate), abbreviated as P(MEOMA--OEGMA), thin film on a hydrophobic polyacrylonitrile (PAN) substrate coating, which resembles a synthetic fabric, is probed by neutron reflectivity (NR). The PAN and monomer (MEOMA and OEGMA) solutions are sequentially spin-coated onto a silicon (Si) substrate. Afterward, plasma treatment is applied to realize the cross-linking of PAN and monomers. The as-prepared cross-linked P(MEOMA--OEGMA) film on the hydrophobic PAN substrate coating presents a two-layer structure: a substrate-near layer, which is a mixture of PAN and P(MEOMA--OEGMA), and a main layer, which is composed of pure hydrophilic P(MEOMA--OEGMA). During hydration in DO vapor atmosphere, the hydrophobic PAN component prevents the formation of DO enrichment in the substrate-near layer. However, an additional vapor-near layer is observed on top of the main layer, which is enriched with DO. The hydration process is constrained by the cross-linking points in the film, inducing the relaxation time to be longer than that in a spin-coated P(MEOMA--OEGMA) film. Because the as-prepared cross-linked film presents a transition temperature (TT) at 38 °C, the hydrated film switches to the collapsed state when the temperature is increased from 23 to 50 °C. The response to a thermal stimulus is also slower due to the existence of the internal cross-linking points as compared to the spin-coated film. Interestingly, no reswelling is observed at the end of the thermal stimulus, which can be also attributed to the presence of internal cross-linking points.
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http://dx.doi.org/10.1021/acs.langmuir.1c00931DOI Listing
June 2021

Management of co-occurring intracranial hemorrhage and fatal pulmonary embolism: a case report.

Chin Med J (Engl) 2021 Jan 11;134(10):1257-1258. Epub 2021 Jan 11.

Department of Pulmonary and Critical Care Medicine, The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu, Zhejiang 322000, China.

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http://dx.doi.org/10.1097/CM9.0000000000001291DOI Listing
January 2021

A Novel Biomarker to Screen for Malnutrition: Albumin/Fibrinogen Ratio Predicts Septic Failure and Acute Infection in Patients Who Underwent Revision Total Joint Arthroplasty.

J Arthroplasty 2021 Apr 30. Epub 2021 Apr 30.

Department of Orthopaedics, The First Medical Centre, Chinese PLA General Hospital, Beijing, China.

Background: This study aimed to investigate the efficacy of the albumin/fibrinogen ratio (AFR) in the assessment of malnutrition and to compare its ability to predict early postoperative periprosthetic joint infection (PJI) in patients with aseptic revisions.

Methods: Four hundred sixty-six patients undergoing revision total hip or knee arthroplasty between February 2017 and December 2019 were recruited in this retrospective study. We compared the differences in nutritional parameters between patients undergoing revision for septic and aseptic reasons. We used multivariate logistic regression and assessed the association between nutritional parameters and risk of PJI. 207 patients with aseptic revision were then evaluated for the incidence of acute postoperative infection within 90 days. The predictive ability of nutritional markers was assessed by receiver operating characteristic curves.

Results: In the multivariate logistic regression analysis, low albumin level (adjusted OR 1.56, 95% CI 1.16-2.08, P = .003), low prognostic nutritional index (PNI) (adjusted OR 1.57, 95% CI 1.01-2.43, P < .043), and low AFR (adjusted OR 2.54, 95% CI 1.92-3.36, P < .001) were independently associated with revision surgery for septic reasons. In accordance with the receiver operating characteristic analysis, the AFR exhibited a greater area under the curve value (0.721) than did the prognostic nutritional index and albumin. An elevated AFR (≥11.7) was significantly associated with old age, joint type, high Charlson comorbidity index, high American Society of Anesthesiologist, and diabetes (P < .05).

Conclusion: Our findings demonstrated AFR may be an effective biomarker to assess nutrition status and predict acute PJIs after revision TJA.
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http://dx.doi.org/10.1016/j.arth.2021.04.027DOI Listing
April 2021

Drug discovery in rheumatoid arthritis with joint effusion identified by text mining and biomedical databases.

Ann Palliat Med 2021 May 7;10(5):5218-5230. Epub 2021 May 7.

Department of Orthopedic Surgery, Chinese PLA General Hospital, Beijing, China; .

Background: Rheumatoid arthritis is a long-term systemic disease that primarily affects multiple synovial joints throughout the body. Some patients with severe joint effusion even require repeated arthrocentesis or arthroscopic debridement to relieve symptoms, which causes them much suffering mentally and physically. This text-mining study was designed to find potential drugs that target key genes in this disease.

Methods: Firstly, we performed text mining by two keywords ("rheumatoid synovitis" and "joint effusion") to get a common set of genes. Secondly, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis performed on these genes, and protein-protein interaction (PPI) network was constructed. Subsequently, the significant genes clustered in the PPI network were chose to execute gene-drug interaction analysis for potential drug discovery.

Results: Through text mining, 68 overlapping genes were identified as an initial set of key genes. Construction of the initial gene set's PPI network showed that 25 genes clustered in a significant gene module. Ultimately, 8 out of 25 genes could be targetable by a total of 19 drugs.

Conclusions: The final 8 genes (PTGS2, TNF, VEGFA, IL1B, CCL2, VWF, IL6, and ESR1) and 19 drugs may provide significant therapeutic value for rheumatoid arthritis patients with joint effusion.
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http://dx.doi.org/10.21037/apm-20-2631bDOI Listing
May 2021

Nonvolatile Electric Control of Exciton Complexes in Monolayer MoSe with Two-Dimensional Ferroelectric CuInPS.

ACS Appl Mater Interfaces 2021 May 11;13(20):24250-24257. Epub 2021 May 11.

International Center for Quantum Design of Functional Materials (ICQD), Hefei National Laboratory for Physical Science at the Microscale, and Synergetic Innovation Center of Quantum Information and Quantum Physics, University of Science and Technology of China, Hefei, Anhui 230026, China.

Monolayer transition-metal dichalcogenides (TMDs) have provided a platform to investigate the excitonic states at the two-dimensional (2D) limit. The inherent properties of excitons in TMDs, such as the photoluminescence quantum yield, the charge states, and even the binding energy, can be effectively controlled via electrostatic gating, selective carrier doping, or substrate dielectric engineering. Here, aiming for the nonvolatile electrical tunability of excitonic states and thereby the optical property of TMDs, we demonstrate a 2D ferroelectric heterostructure with monolayer MoSe and ultrathin CuInPS (CIPS). In the heterostructure, the electric polarization of CIPS results in continuous, global, and large electronic modulation in monolayer MoSe. With the saturated ferroelectric polarization of CIPS, electron-doped or hole-doped MoSe is realized in a single device. The carrier density tunability in the heterostructure is as high as 5 × 10 cm. The nonvolatile behavior of these devices up to 3 months is also characterized. Our results provide a new and practical strategy for low-power consumption and agelong tunable optoelectronic devices.
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http://dx.doi.org/10.1021/acsami.1c03067DOI Listing
May 2021

The effect of microstructure on mechanical properties of corn cob.

Micron 2021 Jul 20;146:103070. Epub 2021 Apr 20.

Key Laboratory of Bionic Engineering, Ministry of Education, Jilin University, Changchun, 130025, China; College of Biological and Agricultural Engineering, Jilin University, Changchun, 130025, China.

As a natural biomass resource, corn cob has excellent mechanical properties and a special layered structure. To investigate the relationship between the mechanical properties and microstructure of corn cob, the ultra-deep field 3D microscope was used to characterize the macro geometric parameters, and the scanning electron microscopy (SEM) was observe the microstructure of the corn cob. The Fourier transform infrared spectrometer was used to analyze the fiber composition, revealing the contribution of fiber composition to the mechanical properties. Axial compression, radial compression, and three-point bending tests were performed on corn cob using a universal testing machine. Moreover, an impact testing machine was used for impact tests. The results show that a corn cob is structurally divided into the pith, woody ring, and glume, mainly composed of cellulose, hemicellulose, and lignin in fiber composition, respectively. The pith is a porous sponge-like tissue that has a greater bearing capacity while maintaining a low density. It is also a progressively hardening material with good buffering properties under impact loads. The woody ring is the primary source of mechanical strength, whose microstructure is a hollow tubular structure composed of cellulose and bonded by lignin. The internal microstructure of the glume is also porous and spongy, but the mechanical properties are mainly manifested in its macrostructure. The results of this study may provide a reference for the subsequent processing and industrial application of corn cob, and the unique structure of corn cob is also an excellent bionic prototype for lightweight design.
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http://dx.doi.org/10.1016/j.micron.2021.103070DOI Listing
July 2021

Redd1 knockdown prevents doxorubicin-induced cardiac senescence.

Aging (Albany NY) 2021 05 6;13(10):13788-13806. Epub 2021 May 6.

Department of Geriatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China.

Regulated in development and DNA damage response-1 (Redd1) is a stress-response gene that is transcriptionally induced by diverse stressful stimuli to influence cellular growth and survival. Although evidence suggests that aging may drive Redd1 expression in skeletal muscles, the expression patterns and functions of Redd1 in senescent cardiomyocytes remain unspecified. To address this issue, and models of cardiomyocyte senescence were established by administration of doxorubicin (Dox). Redd1 overexpression and knockdown was achieved in cultured H9c2 cardiomyocytes and mouse tissues using, respectively, lentivirals and adeno-associated virus 9 (AAV9) vectors. In the hearts of both aged (24 months old) and Dox-treated mice, as well as in Dox-exposed H9c2 cardiomyocytes, high Redd1 expression accompanied the increase in both cellular senescence markers (p16 and p21) and pro-inflammatory cytokine expression indicative of a stress-associated secretory phenotype (SASP). Notably, Redd1 overexpression accentuated, whereas Redd1 silencing markedly attenuated, Dox-induced cardiomyocyte senescence features both and . Notably, AAV9-shRNA-mediated Redd1 silencing significantly alleviated Dox-induced cardiac dysfunction. Moreover, through pharmacological inhibition, immunofluorescence, and western blotting, signaling pathway analyses indicated that Redd1 promotes cardiomyocyte senescence as a downstream effector of p38 MAPK to promote NF-kB signaling via p65 phosphorylation and nuclear translocation.
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http://dx.doi.org/10.18632/aging.202972DOI Listing
May 2021

Three-Dimensional-Printable Thermo/Photo-Cross-Linked Methacrylated Chitosan-Gelatin Hydrogel Composites for Tissue Engineering.

ACS Appl Mater Interfaces 2021 May 7;13(19):22902-22913. Epub 2021 May 7.

Physik-Department, Lehrstuhl für Funktionelle Materialien, Technische Universität München, James-Franck-Str. 1, 85748 Garching, Germany.

Biomimetic constructs imitating the functions, structures, and compositions of normal tissues are of great importance for tissue repair and regeneration. Three-dimensional (3D) printing is an innovative method to construct intricate biomimetic 3D tissue engineering scaffolds with spatiotemporal deposition of materials to control the intrinsic architectural organization and functional performance of the scaffold. However, due to the lack of bioinks with suitable printability, high structural integrity, and biological compatibility, producing constructs that mimic the anisotropic 3D extracellular environments remains a challenge. Here, we present a printable hydrogel ink based on methylacrylate-modified chitosan (ChMA) and gelatin (GelMA) embedding nanohydroxyapatite (nano-Hap). This polymer composite is first physically cross-linked by thermal gelation for postprinting structural stability, followed by covalent photo-cross-linking of ChMA and GelMA to form a long-term stable structure. The rheological behavior of the hydrogels and the mechanical strengths of the printed constructs are tuned by adjusting the content of GelMA, which in turn enhances the shape retention after printing and enables the precise deposition of multilayered 3D scaffolds. Moreover, the formulated biomaterial inks exhibit biological characteristics that effectively support the spreading and proliferation of stem cells seeded on the scaffolds after 7 days of in vitro culture. Adding Hap has minor influences on the mechanical rigidity and cytocompatibility of the hydrogels compared with the group free of Hap. Together, the printable biomaterial inks with shear thinning and good structural integrity, along with biological cues, are promising for tissue engineering application.
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http://dx.doi.org/10.1021/acsami.1c01321DOI Listing
May 2021

Single-Layer MoS Grown on Atomically Flat SrTiO Single Crystal for Enhanced Trionic Luminescence.

ACS Nano 2021 May 5;15(5):8610-8620. Epub 2021 May 5.

The elaborate interface interactions can be critical in determining the achievable functionality of a semiconductor heterojunction (SH), particularly when two-dimensional material is enclosed in the system and its thickness is at an atomic extreme. In this work, we have successfully constructed a SH model system composed of typical transition-metal chalcogenide (TMDs) and transition metal oxides (TMO) by directly growing molybdenum sulfide (MoS) nanosheets on atomically flat strontium titanate (SrTiO) single crystal substrates through a conventional chemical vapor deposition (CVD) synthetic method. Multiple measurements have demonstrated the uniform monolayer thickness and single crystallinity of the MoS nanosheets as well as the atomic flatness of the heterojunction surface, both characterizing an extremely high quality of the interface. Clear evidence have been obtained for the electron transfer from the MoS adlayer to the SrTiO substrate which varies against the interface conditions. More importantly, the photoluminescence of MoS is significantly tailored, which is correlated with both the cleanness of the interface and the crystal orientation of the SrTiO substrate. These results not only shed fresh lights on the structure-property relationship of the TMDs/TMO heterostructures but also manifest the importance of the ideal interface structure for a hybridized system.
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http://dx.doi.org/10.1021/acsnano.1c00482DOI Listing
May 2021

Positive frozen section during reimplantation increases the risk of treatment failure in two-stage exchange arthroplasty even in patients with normal ESR and CRP.

Bone Joint J 2021 May;103-B(5):916-922

Medical School of Chinese PLA, Beijing, China.

Aims: It can be extremely challenging to determine whether to perform reimplantation in patients who have contradictory serum inflammatory markers and frozen section results. We investigated whether patients with a positive frozen section at reimplantation were at a higher risk of reinfection despite normal ESR and CRP.

Methods: We retrospectively reviewed 163 consecutive patients with periprosthetic joint infections (PJIs) who had normal ESR and CRP results pre-reimplantation in our hospital from 2014 to 2018. Of these patients, 26 had positive frozen sections at reimplantation. The minimum follow-up time was two years unless reinfection occurred within this period. Univariable and multivariable logistic regression analyses were performed to identify the association between positive frozen sections and treatment failure.

Results: Treatment failure occurred in eight (30.77%) of the 26 PJI patients with positive frozen sections at reimplantation, compared with 13 (9.49%) of 137 patients with negative results. In the multivariate analysis, positive frozen section increased the risk of failure (odds ratio 4.70; 95% confidence interval (CI) 1.64 to 13.45). The mean number of months to reinfection was lower in the positive frozen section group than in the control group (p = 0.041). While there were nine (34.62%) patients with positive frozen section and 25 (18.25%) patients with negative frozen section who had prolonged antibiotic use (p = 0.042), the mean duration of antibiotic use was comparable in two groups. Synovial white blood cell count (p = 0.137) and polymorphonuclear leucocyte percentage (p = 0.454) were not associated with treatment failure in logistic regression model.

Conclusion: Positive frozen section at reimplantation was independently associated with subsequent failure and earlier reinfection, despite normal ESR and CRP levels pre-reimplantation. Surgeons should be aware of the risk of treatment failure in patients with positive frozen sections and carefully consider benefits of reimplantation. Cite this article:  2021;103-B(5):916-922.
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http://dx.doi.org/10.1302/0301-620X.103B5.BJJ-2020-0703.R3DOI Listing
May 2021

Tissue adhesive hydrogel bioelectronics.

J Mater Chem B 2021 Jun;9(22):4423-4443

Key Laboratory of Polymeric Composite and Functional Materials of Ministry of Education, Guangdong Functional Biomaterials Engineering Technology Research Center, Guangzhou Key Laboratory of Flexible Electronic Materials and Wearable Devices, School of Materials Science and Engineering, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, China.

Flexible bioelectronics have promising applications in electronic skin, wearable devices, biomedical electronics, etc. Hydrogels have unique advantages for bioelectronics due to their tissue-like mechanical properties and excellent biocompatibility. Particularly, conductive and tissue adhesive hydrogels can self-adhere to bio-tissues and have great potential in implantable wearable bioelectronics. This review focuses on the recent progress in tissue adhesive hydrogel bioelectronics, including the mechanism and preparation of tissue adhesive hydrogels, the fabrication strategies of conductive hydrogels, and tissue adhesive hydrogel bioelectronics and applications. Some perspectives on tissue adhesive hydrogel bioelectronics are provided at the end of the review.
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http://dx.doi.org/10.1039/d1tb00523eDOI Listing
June 2021

Reconstruction with customized, 3D-printed prosthesis after resection of periacetabular Ewing's sarcoma in children using "triradiate cartilage-based" surgical strategy:a technical note.

J Orthop Translat 2021 May 26;28:108-117. Epub 2021 Mar 26.

Department of Orthopedic Surgery, Xi-jing Hospital, Air Force Medical University, Xi'an, 710032, China.

Background: Surgery for Ewing sarcoma involving acetabulum in children is challenging. Considering the intrinsic structure of immature pelvis, trans-acetabular osteotomy through triradiate cartilage might be applied. The study was to describe the surgical technique and function outcomes of trans-acetabular osteotomy through triradiate cartilage and reconstruction with customized, 3D-printed prosthesis.

Methods: Two children with periacetabular ES were admitted to our hospital. The pre-operative imaging showed the triradiate cartilage was not penetrated or wholly affected by tumor. After neoadjuvant chemotherapy, the tumor was excised by trans-acetabular osteotomy basing on "triradiate cartilage strategy" and the acetabulum was reconstructed with the customized, 3D-printed prosthesis. The prosthesis was designed in Mimics software basing on the images from CT, optimized by topology technique, and examined in FE model. After implantation, the oncological and functional outcomes were evaluated with radiography, CT, and MSTS score.

Results: The operation time and intra-operative blood loss in these two children were 3.5h, 2.5h and 300 ml, 600 ml, respectively. The postoperative specimen showed the tumor was en bloc removed with safe margin. In the latest follow-up (48 months and 24 months), both patients were free of disease and had satisfactory function according to MSTS score. The radiography indicated the prosthesis fit the defect well without loosening.

Conclusion: The customized, 3D-printed prosthesis could provide optimal reconstruction of pelvic ring and satisfactory hip function after trans-acetabular osteotomy in children.

The Translational Potential Of This Article: This study provides promising results of implantation of customized 3D printing prosthesis in children's pelvic sarcoma, which may bring a new design method for orthopaedic implants.
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http://dx.doi.org/10.1016/j.jot.2020.12.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022806PMC
May 2021

Genome-wide association mapping for resistance to bacterial blight and bacterial leaf streak in rice.

Planta 2021 Apr 8;253(5):94. Epub 2021 Apr 8.

Southern Regional Collaborative Innovation Center for Grain and Oil Crops and College of Agronomy, Hunan Agricultural University, Changsha, China.

Main Conclusion: Using genome-wide SNP association mapping, a total of 77 and 7 loci were identified for rice bacterial blight and bacterial leaf streak resistance, respectively, which may facilitate rice resistance improvement. Bacterial blight (BB) and bacterial leaf streak (BLS) caused by Gram-negative bacteria Xanthomonas oryzae pv. oryzae (Xoo) and X. oryzae pv. oryzicola (Xoc), respectively, are two economically important diseases negatively affecting rice production. To mine new sources of resistance, a set of rice germplasm collection consisting of 895 re-sequenced accessions from the 3000 Rice Genomes Project (3 K RGP) were screened for BB and BLS resistance under field conditions. Higher levels of BB resistance were observed in aus/boro subgroup, whereas the japonica, temperate japonica and tropical japonica subgroups possessed comparatively high levels of resistance to BLS. A genome-wide association study (GWAS) mined 77 genomic loci significantly associated with BB and 7 with BLS resistance. The phenotypic variance (R) explained by these loci ranged from 0.4 to 30.2%. Among the loci, 7 for BB resistance were co-localized with known BB resistance genes and one for BLS resistance overlapped with a previously reported BLS resistance QTL. A search for the candidates in other novel loci revealed several defense-related genes that may be involved in resistance to BB and BLS. High levels of phenotypic resistance to BB or BLS could be attributed to the accumulation of the resistance (R) alleles at the associated loci, indicating their potential value in rice resistance breeding via gene pyramiding. The GWAS analysis validated the known genes underlying BB and BLS resistance and identified novel loci that could enrich the current resistance gene pool. The resources with strong resistance and significant SNPs identified in this study are potentially useful in breeding for BB and BLS resistance.
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http://dx.doi.org/10.1007/s00425-021-03612-5DOI Listing
April 2021

Trends in microbiological profiles and antibiotic resistance in periprosthetic joint infections.

J Int Med Res 2021 Mar;49(3):3000605211002784

Medical School of Chinese PLA, Beijing, China.

Objective: This study examined the trends in demographics, the distribution of microorganisms, and antibiotic resistance in patients with periprosthetic joint infection (PJI).

Methods: We conducted a retrospective study of 231 consecutive patients diagnosed with PJI in our hospital from January 2006 to December 2015 (93 and 138 patients diagnosed in 2006-2010 and 2011-2015, respectively). The linear-by-linear chi-squared test was used to assess the trends in demographics, the distribution of microorganisms, and antibiotic resistance.

Results: Gram-positive cocci accounted for 63.9% of all pathogens, and coagulase-negative (CoNS) accounted for 38.1% of all isolates. The proportion of isolates identified as methicillin-resistant CoNS significantly increased over the study period (39.0% vs. 61.8%). In addition, the proportions of levofloxacin-resistant CoNS (4.9% vs. 21.8%) and (6.3% vs. 45.0%) isolates significantly increased over the study period. By contrast, the proportions of penicillin-resistant CoNS (82.9% vs. 40.0%) and (75.0% vs. 30.0%) isolates decreased over the study period.

Conclusion: Our research revealed changes in the distribution of microorganisms and antibiotic resistance profile of the pathogens responsible for PJI over time, which could complicate treatment. These findings may serve as a reference for strategies to prevent and empirically treat PJI in China.
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http://dx.doi.org/10.1177/03000605211002784DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020254PMC
March 2021

Deciphering the metabolic profile and pharmacological mechanisms of Achyranthes bidentata blume saponins using ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry coupled with network pharmacology-based investigation.

J Ethnopharmacol 2021 Jun 23;274:114067. Epub 2021 Mar 23.

Anhui University of Chinese Medicine, Hefei, 230012, China; Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui Province Key Laboratory of R&D of Chinese Medicine, Hefei, 230012, China; Anhui Province Key Laboratory of Chinese Medicinal Formula, Hefei, 230012, China.

Ethnopharmacological Relevance: Achyranthes bidentata Blume (AB) is a traditional Chinese medicine (TCM) widely used as a dietary supplement and anti-arthritis drug. Pharmacological studies have shown that Achyranthes bidentata Blume saponins (ABS) are the main bioactive ingredient. However, the metabolic profile and mechanisms of action of ABS against rheumatic arthritis (RA) remain to be established.

Aim Of The Study: Our main objective was to investigate the metabolic profile and pharmacological activities of ABS against RA.

Materials And Methods: In this study, an analytical method based on ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) coupled with a metabolism platform was developed for metabolic profiling of ABS in rat liver microsomes and plasma. Then, the in vivo metabolites of ABS and their targets associated with RA were used to construct the network pharmacological analysis. Gene ontology (GO) enrichment, KEGG signaling pathway analyses and pathway network analyses were performed. The therapeutic effect of ABS on RA was further evaluated using an adjuvant arthritis (AA) model and network pharmacology results validated via Western blot.

Results: Overall, 26 and 21 metabolites of ABS were tentatively characterized in rat liver microsomes and plasma, respectively. The metabolic pathways of ABS mainly included M+O, M+O-H, M+O, and M+O-H. Data form network pharmacology analysis suggested that MAPK, apoptosis, PI3K-AKT and p53 signaling pathways contribute significantly to the therapeutic effects of ABS on RA. In pharmacodynamics experiments, ABS ameliorated the symptoms in AA rats in a dose-dependent manner and restored the homeostasis of pro/anti-inflammatory factors. Western blot results further demonstrated a significant ABS-induced decrease in phosphorylation of ERK in the MAPK pathway (P < 0.01).

Conclusion: Application of an analytical method based on UPLC-QTOF/MS, network pharmacology and validation experiments offers novel insights into the components and mechanisms of ABS that contribute to its therapeutic effects against RA, providing useful directions for further research.
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http://dx.doi.org/10.1016/j.jep.2021.114067DOI Listing
June 2021

Adaptive Finite-Time Tracking Control of Nonholonomic Multirobot Formation Systems With Limited Field-of-View Sensors.

IEEE Trans Cybern 2021 Mar 23;PP. Epub 2021 Mar 23.

This article studies the vision-based tracking control problem for a nonholonomic multirobot formation system with uncertain dynamic models and visibility constraints. A fixed onboard vision sensor that provides the relative distance and bearing angle is subject to limited range and angle of view due to limited sensing capability. The constraint resulting from collision avoidance is also taken into account for safe operations of the formation system. Furthermore, the preselected specifications on transient and steady-state performance are provided by considering the time-varying and asymmetric constraint requirements on formation tracking errors for each robot. To address the constraint problems, we incorporate a novel barrier Lyapunov function into controller design and analysis. Based on the recursive adaptive backstepping procedure and neural-network approximation, we develop a vision-based formation tracking control protocol such that formation tracking errors can converge into a small neighborhood of the origin in finite time while meeting the requirements of visibility and performance constraints. The proposed protocol is decentralized in the sense that the control action on each robot only depends on the local relative information, without the need for explicit network communication. Moreover, the control protocol could extend to an unconstrained multirobot system. Both simulation and experimental results show the effectiveness of the control protocol.
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http://dx.doi.org/10.1109/TCYB.2021.3063481DOI Listing
March 2021

Validating a segment on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety.

Sci Rep 2021 Mar 15;11(1):6053. Epub 2021 Mar 15.

Key Laboratory of Southern Rice Innovation and Improvement, Ministry of Agriculture and Rural Affairs, Hunan Engineering Laboratory of Disease and Pest Resistant Rice Breeding, Yuan Longping High-Tech Agriculture Co., Ltd., Changsha, 410128, China.

Cadmium (Cd) contamination of rice is a serious food safety issue that has recently been gaining significant public attention. Therefore, reduction of Cd accumulation in rice grains is an important objective of rice breeding. The use of favourable alleles of Cd accumulating genes using marker-assisted selection (MAS) is theoretically feasible. In this study, we validated a segment covering OsHMA3-OsNramp5-OsNramp1 on chromosome 7 of japonica for establishing low-cadmium accumulating indica rice variety. The OsHMA3-OsNramp5-OsNramp1 haplotype significantly decreased grain Cd concentration in middle-season indica genetic background. The improved 9311 carrying the OsHMA3-OsNramp5-OsNramp1 haplotype with recurrent parent genome recovery of up to 91.6% resulted in approximately 31.8% decrease in Cd accumulation in the grain and with no penalty on yield. There is a genetic linkage-drag between OsHMA3-OsNramp5-OsNramp1 and the gene conditioning heading to days (HTD) in the early-season indica genetic background. Because the OsHMA3-OsNramp5-OsNramp1-Ghd7 haplotype significantly increases grain Cd concentration and prolongs growth duration, the linkage-drag between OsHMA3-OsNramp5-OsNramp1 and Ghd7 should be broken down by large segregating populations or gene editing. A novel allele of OsHMA3 was identified from a wide-compatibility japonica cultivar, the expression differences of OsNramp1 and OsNramp5 in roots might contribute the Cd accumulating variation between japonica and indica variety.
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http://dx.doi.org/10.1038/s41598-021-85324-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7961068PMC
March 2021

Pre- and Postoperative Models for Prediction of Recurrence in Non-B, Non-C Hepatocellular Carcinoma.

Front Oncol 2021 18;11:612588. Epub 2021 Feb 18.

Department of Hepatopancreatobiliary Surgery, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, China.

Background And Aims: The incidence of non-B, non-C hepatocellular carcinoma (NBNC-HCC) is increasing. Like in hepatitis B virus (HBC)/HCV-associated HCC, treatment of NBNC-HCC after resection is challenging due to its high recurrence rate. However, few studies on the recurrence of NBNC-HCC have been published in the past decades. Hence, we aimed to investigate the risk factors for recurrence of NBNC-HCC and construct pre- and postoperative prognostic models for predicting recurrence in these patients who underwent curative resection.

Methods: We retrospectively analyzed 608 patients who underwent liver resection for NBNC-HCC. A multivariate Cox proportional hazard regression analysis was conducted to identify the independent risk factors of recurrence, based on which the prediction nomogram models were constructed and validated. The predictive performance of the models was assessed using the concordance index, time-dependent receiver operating characteristic curve, prediction error cure, and calibration curve. To facilitate clinical use, we stratified the patients into three distinct risk groups based on the score of the models. The cutoff scores of the models were determined by a survival tree analysis.

Results: Multivariable analysis identified neutrophil-to-lymphocyte ratio, alpha fetoprotein, tumor number, and tumor diameter as independent preoperative risk factors for recurrence. In addition to these variables, microvascular invasion was an independent postoperative risk factor for recurrence. The pre- and postoperative nomograms were constructed based on these variables. The C-index of the pre- and postoperative nomograms was 0.689 and 0.702 in the training cohort, 0.682 and 0.688 in the validation cohort, respectively, which were both higher than those of the conventional Barcelona Clinic Liver Cancer (BCLC) and 8 edition of the American Joint Committee on Cancer (AJCC) staging systems. In addition, the pre- and postoperative nomograms could also re-stratify patients with BCLC stage 0/A or AJCC stage IA/IB/II into distinct risk groups.

Conclusions: We constructed pre- and postoperative prognostic models for predicting recurrence in patients with NBNC-HCC who underwent curative resection. They can play a supplementary role to the traditional staging system.
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http://dx.doi.org/10.3389/fonc.2021.612588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7930483PMC
February 2021

Fractional-Order Adaptive Fault-Tolerant Synchronization Tracking Control of Networked Fixed-Wing UAVs Against Actuator-Sensor Faults via Intelligent Learning Mechanism.

IEEE Trans Neural Netw Learn Syst 2021 Mar 4;PP. Epub 2021 Mar 4.

This article presents an enhanced fault-tolerant synchronization tracking control scheme using fractional-order (FO) calculus and intelligent learning architecture for networked fixed-wing unmanned aerial vehicles (UAVs) against actuator and sensor faults. To increase the flight safety of networked UAVs, a recurrent wavelet fuzzy neural network (RWFNN) learning system with feedback loops is first designed to compensate for the unknown terms induced by the inherent nonlinearities, unexpected actuator, and sensor faults. Then, FO sliding-mode control (FOSMC), involving the adjustable FO operators and the robustness of SMC, are dexterously proposed to further enhance flight safety and reduce synchronization tracking errors. Moreover, the dynamic parameters of the RWFNN learning system embedded in the networked fixed-wing UAVs are updated based on adaptive laws. Furthermore, the Lyapunov analysis ensures that all fixed-wing UAVs can synchronously track their references with bounded tracking errors. Finally, comparative simulations and hardware-in-the-loop experiments are conducted to demonstrate the validity of the proposed control scheme.
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http://dx.doi.org/10.1109/TNNLS.2021.3059933DOI Listing
March 2021

Distributed Fractional-Order Intelligent Adaptive Fault-Tolerant Formation-Containment Control of Two-Layer Networked Unmanned Airships for Safe Observation of a Smart City.

IEEE Trans Cybern 2021 Feb 26;PP. Epub 2021 Feb 26.

This article investigates a distributed fractional-order fault-tolerant formation-containment control (FOFTFCC) scheme for networked unmanned airships (UAs) to achieve safe observation of a smart city. In the proposed control method, an interval type-2 fuzzy neural network (IT2FNN) is first developed for each UA to approximate the unknown term associated with the loss-of-effectiveness faults in the distributed error dynamics, and then a disturbance observer (DO) is proposed to compensate for the approximation error and bias fault encountered by each UA, such that the composite learning strategy composed of the IT2FNN and the DO is obtained for each UA. Moreover, fractional-order (FO) calculus is incorporated into the control scheme to provide an extra degree of freedom for the parameter adjustments. The salient feature of the proposed control scheme is that the composite learning algorithm and FO calculus are integrated to achieve a satisfactory fault-tolerant formation-containment control performance even when a portion of leader/follower UAs is subjected to the actuator faults in a distributed communication network. Furthermore, it is shown by Lyapunov stability analysis that all leader UAs can track the virtual leader UA with time-varying offset vectors, and all follower UAs can converge into the convex hull spanned by the leader UAs. Finally, comparative hardware-in-the-loop (HIL) experimental results are presented to show the effectiveness and superiority of the proposed method.
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http://dx.doi.org/10.1109/TCYB.2021.3052875DOI Listing
February 2021

Multifunctional conductive hydrogels and their applications as smart wearable devices.

J Mater Chem B 2021 03 18;9(11):2561-2583. Epub 2021 Feb 18.

State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.

Recently, hydrogel-based conductive materials and their applications as smart wearable devices have been paid tremendous attention due to their high stretchability, flexibility, and excellent biocompatibility. Compared with single functional conductive hydrogels, multifunctional conductive hydrogels are more advantageous to match various demands for practical applications. This review focuses on multifunctional conductive hydrogels applied for smart wearable devices. Representative strategies for conduction of hydrogels are discussed firstly: (1) electronic conduction based on the conductive fillers and (2) ionic conduction based on charged ions. Then, the common and intensive research on multiple functionalities of conductive hydrogels, such as mechanical properties, conductive and sensory properties, anti-freezing and moisturizing properties, and adhesion and self-healing properties is presented. The applications of multifunctional conductive hydrogels such as in human motion sensors, sensory skins, and personal healthcare diagnosis are provided in the third part. Finally, we offer our perspective on open challenges and future areas of interest for multifunctional conductive hydrogels used as smart wearable devices.
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http://dx.doi.org/10.1039/d0tb02929gDOI Listing
March 2021

Leukocyte esterase test and alpha-defensin test have similar accuracy for the diagnosis of periprosthetic joint infection.

Int Orthop 2021 Feb 12. Epub 2021 Feb 12.

Department of Orthopaedics, Chinese PLA General Hospital (301 Hospital), 28 Fuxing Rd., Beijing, 100853, People's Republic of China.

Background: The diagnosis of periprosthetic joint infection (PJI) after total joint replacement remains challenging. Synovial biomarkers are recommended as the major diagnostic criteria for PJI. The purpose of this study was to test the accuracy of the alpha-defensin test and compare it with that of the leukocyte esterase (LE) test for the diagnosis of PJI.

Methods: We obtained 130 hip or knee aspirates from May 2015 to September 2018. PJI was defined according to the European Bone and Joint Infection Society (EBJIS) criteria. Synovial fluid samples were tested with the alpha-defensin ELISA and a LE strip. For the LE strip test, besides using the traditional threshold 500 (equal to ++), we are also using an improved one, with 500 (equal to ++) serving as the threshold for the tests before centrifugation and both 250 and 500 indicating positive results after centrifugation. The receiver operating characteristic (ROC) curves, sensitivity, specificity, positive predictive value, negative predictive value, and Cohen's Kappa value were calculated for the LE and alpha-defensin tests.

Results: The area under the curve (AUC) of alpha-defensin, LE strip test with traditional, and improved interpretation strategy were 0.875, 0.854, and 0.877, respectively. The Cohen's Kappa value for the alpha-defensin tests was 0.826 with the traditional LE interpretation strategy and 0.875 with the improved strategy.

Conclusion: The present study shows that the use of the LE strip to test synovial fluid yielded an accuracy similar to that of the alpha-defensin immunoassay for the diagnosis of PJI; this finding supports the 2018 ICM PJI definition, which considers positive alpha-defensin and LE test results to be equivalent.
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http://dx.doi.org/10.1007/s00264-020-04903-4DOI Listing
February 2021

Dynamic Learning From Adaptive Neural Control for Discrete-Time Strict-Feedback Systems.

IEEE Trans Neural Netw Learn Syst 2021 Feb 8;PP. Epub 2021 Feb 8.

This article first investigates the issue on dynamic learning from adaptive neural network (NN) control of discrete-time strict-feedback nonlinear systems. To verify the exponential convergence of estimated NN weights, an extended stability result is presented for a class of discrete-time linear time-varying systems with time delays. Subsequently, by combining the n-step-ahead predictor technology and backstepping, an adaptive NN controller is constructed, which integrates the novel weight updating laws with time delays and without the σ modification. After ensuring the convergence of system output to a recurrent reference signal, the radial basis function (RBF) NN is verified to satisfy the partial persistent excitation condition. By the combination of the extended stability result, the estimated NN weights can be verified to exponentially converge to their ideal values. The convergent weight sequences are comprehensively represented and stored by constructing some elegant learning rules with some novel sequences and the mod function. The stored knowledge is used again to develop a neural learning control scheme. Compared with the traditional adaptive NN control, the proposed scheme can not only accomplish the same or similar tracking tasks but also greatly improve the transient control performance and alleviate the online computation. Finally, the validity of the presented scheme is illustrated by numerical and practical examples.
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http://dx.doi.org/10.1109/TNNLS.2021.3054378DOI Listing
February 2021

Transanal Opening of Intersphincteric Space for Fistula-in-Ano.

Am Surg 2021 Jan 30:3134821989048. Epub 2021 Jan 30.

Colorectal Surgery Division, The People Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China.

Background: The role of the intersphincteric space in the pathogenesis of fistula-in-ano is being increasingly recognized. Submucosal and intersphincteric rectal abscesses have been surgically managed by laying open and draining the intersphincteric space as well as by the modified ligation of intersphincteric fistula tract (LIFT) procedure. In 2017, the transanal opening of intersphincteric space (TROPIS) technique was reported for the treatment of high, complex anal fistulae.

Aim: We aim to investigate the advantages of performing the TROPIS procedure in patients with fistula-in-ano.

Methods: This was a prospective cohort study investigating the outcomes in patients who had undergone a procedure using the TROPIS technique for the treatment of fistula-in-ano. Preoperative magnetic resonance imaging scans and electronic colonoscopies were performed on all patients. A clinical database evaluating the following variables was constructed: age, gender, body mass index (BMI), previous fistula surgery, type of fistula, postoperative complications, duration of follow-up, success rate, and incontinence scores pre- and postoperatively.

Results: The TROPIS procedure was performed on 41 patients with fistula-in-ano with a follow-up time of 6-23 months. The characteristics of the patients were as follows: 36 males, 6 females, mean age 38.6±13.2 years, and mean BMI 23.5±3.9 kg·m. All patients (41) had transsphincteric fistulae, and 90.2% (37) had high fistula. Of the 41 patients, 22% (9) had recurrent fistulae, 29.27% (12) had horseshoe fistulae, 7.3% (3) had supralevator fistulae, and 14.6% (6) had an associated abscess. The fistula healed completely in 85.3% (35) of patients and failed to heal in 14.7% (6) of patients, and the healing of high fistula was 86.5% (32). Of those patients who had not healed completely, 2 were found to have contracted iatrogenic infections due to foreign residues and underwent surgery with the passing of a loose seton. The additional 4 patients who had not healed underwent a fistulotomy and healed completely thereafter. There were no significant changes in incontinence scores. The incontinence scores were .15 ± .36 preoperatively and .22 ± .47 3 months postoperatively (t = -1.438, = .16).

Conclusions: The TROPIS technique is a novel sphincter-preserving procedure, which can be effectively used in treating fistula-in-ano.
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http://dx.doi.org/10.1177/0003134821989048DOI Listing
January 2021

[Recombineering facilitates the discovery of natural product biosynthetic pathways in Pseudomonas parafulva].

Biotechnol J 2021 Jan 23:e2000575. Epub 2021 Jan 23.

Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, 410081, People's Republic of China.

Microbial natural products among other functions they play a vital role in the disease prevention in humans, animals and plants. Pseudomonas parafulva CRS01-1 is a broad-spectrum antagonistic bacterium present in plants. However, no natural products have been isolated from this strain till date. Corresponding biosynthetic gene clusters to natural products is an effective method for bioprospecting, for which, genome manipulation tools are essential. We previously developed Pseudomonas-specific phage-derived homologous recombination systems for genetic engineering in four Pseudomonas species. Herein, we report the application of these recombineering systems in Pseudomonas parafulva CRS01-1, along with structural elucidation and bioactivity evaluation of natural products. The Pseudomonas recombineering toolbox established before in different four species is efficient for genome mining and bioactive metabolite discovery from more distant species. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/biot.202000575DOI Listing
January 2021

Spreading of TDP-43 pathology via pyramidal tract induces ALS-like phenotypes in TDP-43 transgenic mice.

Acta Neuropathol Commun 2021 01 18;9(1):15. Epub 2021 Jan 18.

Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.

Transactive response DNA-binding protein 43 kDa (TDP-43) has been identified as the major component of ubiquitinated inclusions found in patients with sporadic amyotrophic lateral sclerosis (ALS). Increasing evidence suggests prion-like transmission of TDP-43 aggregates via neuroanatomic connection in vitro and pyramidal tract in vivo. However, it is still unknown whether the spreading of pathological TDP-43 sequentially via pyramidal tract can initiate ALS-like pathology and phenotypes. In this study, we reported that injection of TDP-43 preformed fibrils (PFFs) into the primary motor cortex (M1) of Thy1-e (IRES-TARDBP) 1 mice induced the spreading of pathological TDP-43 along pyramidal tract axons anterogradely. Moreover, TDP-43 PFFs-injected Thy1-e (IRES-TARDBP) 1 mice displayed ALS-like neuropathological features and symptoms, including motor dysfunctions and electrophysiological abnormalities. These findings provide direct evidence that transmission of pathological TDP-43 along pyramidal tract induces ALS-like phenotypes, which further suggest the potential mechanism for TDP-43 proteinopathy.
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http://dx.doi.org/10.1186/s40478-020-01112-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7814549PMC
January 2021

Application of a novel thermo-sensitive injectable hydrogel in therapy in situ for drug accurate controlled release.

J Biomed Mater Res B Appl Biomater 2020 11 17;108(8):3200-3216. Epub 2020 Jul 17.

College of Chemistry and Pharmaceutical Science, Qingdao Agricultural University, Qingdao, China.

Local drug injection therapy for tumor site, as a neoadjuvant chemotherapy method, shows important significance in clinical application; however, it obtains unsatisfying therapeutic effect due to the serious toxic and side effect in normal tissues caused by drug diffusion or complexity of the preparation. In this article, the influence factors of the gelling time of traditional Chitosan (CTS) thermo-sensitive hydrogels were analyzed, and the gelling properties were improved significantly, and a thermo-sensitive hydrogel with precisely regulated gelling time was obtained through a green and simple preparation method, and the shortest gelling time (gelling time = 27 ± 2 s) of this hydrogel was 5% of that of the common CTS thermo-sensitive hydrogels. After loaded with different chemotherapy drugs with different pH values (gemctiabin hydrochloride, levofloxacin, and 5-foluorouracil), the hydrogels' gelling performance was not affected, while the gelling time could be shortened by 5-foluorouracil, effectively hindering the drug loss at the early stage of sustained release. in vitro and in vivo experiments proved that precise encapsulation toward tumors with different volumes was achieved by the hydrogels, with minimal damage to surrounding normal tissues and higher utilization of drugs in tumor sites, ultimately achieving better tumor therapeutic effect. In conclusion, the new thermo-sensitive hydrogels with precisely regulated gelling time showed great significance and potential for drug delivery and neoadjuvant chemotherapy.
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http://dx.doi.org/10.1002/jbm.b.34658DOI Listing
November 2020

Complete mitochondrial genome of (Coleoptera: Curculionidae).

Mitochondrial DNA B Resour 2020 Jun 11;5(3):2403-2405. Epub 2020 Jun 11.

College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang, China.

Chao is an important insect pest on many plants which belong to genus The complete mitochondrial genome of was sequenced and analyzed. The phylogenetic relationships between and other 41 species in the family Curculionidae were reconstructed using maximum likelihood (ML) methods based on the concatenated nucleotide sequences, the phylogenetic analysis showed that is closely related to , which is in accordance with the traditional morphological classification.
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http://dx.doi.org/10.1080/23802359.2020.1772683DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7781971PMC
June 2020

The effect of environmental conditions on the rate of RNA degradation in dried blood stains.

Forensic Sci Int Genet 2021 Mar 28;51:102456. Epub 2020 Dec 28.

School of Forensic Sciences, Oklahoma State University Center for Health Sciences Tulsa, OK, United States. Electronic address:

The relationship between RNA degradation and the age of a bloodstain has been suggested by the work of several investigators. A prior study from this laboratory described a qPCR assay that was effective at estimating the age of bloodstains stored in an environmentally controlled laboratory for periods of up to one year. In this study, the effect of the environmental conditions on the rate of RNA degradation during storage was analyzed. Bloodstains were prepared on stain cards and stored in one of 9 different environments for periods of up to 24 weeks. At selected times during the storage term, RNA was extracted, reverse transcribed, and the integrity of select transcripts analyzed. Three temperatures (37 °C, 20 °C, and 4 °C) and three relative humidities (rH) (75 %, 35 %, and 10 %) were combined pairwise. The rate of RNA degradation was found to increase 5-10 fold in stains stored at 37 °C versus those stored at 20 °C. The rate of RNA degradation was faster for stains stored at 20 °C compared to 4 °C but differed only 2-4 fold. Multivariate regression analysis suggests elevations in temperature or rH will accelerate RNA degradation and will do so to a similar extent. It is clear from the data that the integrity of the transcriptome in dried bloodstains is better preserved in a cold and dry environment. Investigations are ongoing to develop an approach for the estimation of sample age that incorporates the environmental conditions of a crime scene into the age estimate.
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http://dx.doi.org/10.1016/j.fsigen.2020.102456DOI Listing
March 2021

Genome structure variation analyses of peach reveal population dynamics and a 1.67 Mb causal inversion for fruit shape.

Genome Biol 2021 Jan 5;22(1):13. Epub 2021 Jan 5.

Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, People's Republic of China.

Background: Structural variations (SVs), a major resource of genomic variation, can have profound consequences on phenotypic variation, yet the impacts of SVs remain largely unexplored in crops.

Results: Here, we generate a high-quality de novo genome assembly for a flat-fruit peach cultivar and produce a comprehensive SV map for peach, as a high proportion of genomic sequence is occupied by heterozygous SVs in the peach genome. We conduct population-level analyses that indicate SVs have undergone strong purifying selection during peach domestication, and find evidence of positive selection, with a significant preference for upstream and intronic regions during later peach improvement. We perform a SV-based GWAS that identifies a large 1.67-Mb heterozygous inversion that segregates perfectly with flat-fruit shape. Mechanistically, this derived allele alters the expression of the PpOFP2 gene positioned near the proximal breakpoint of the inversion, and we confirm in transgenic tomatoes that PpOFP2 is causal for flat-fruit shape.

Conclusions: Thus, beyond introducing new genomics resources for peach research, our study illustrates how focusing on SV data can drive basic functional discoveries in plant science.
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http://dx.doi.org/10.1186/s13059-020-02239-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7784018PMC
January 2021