Publications by authors named "Juliana Araújo de Carvalho Schettini"

2 Publications

  • Page 1 of 1

Evaluation of Immunological Parameters in Pregnant Women: Low Levels of B and NK Cells.

Rev Bras Ginecol Obstet 2019 Apr 25;41(4):213-219. Epub 2019 Mar 25.

Instituto de Medicina Integral Professor Fernando Figueira, Recife, PE, Brazil.

Objective:  To describe the immunological and hematological reference intervals of low-risk pregnant women.

Methods:  A cross-sectional retrospective database analysis of a basic and translational study analyzing the hematological evaluation blood counts and immunophenotyping of TCD3 + , TCD4 + , TCD8 + , B, and natural killer (NK) cells of the peripheral blood in 79 low-risk pregnant women and of 30 control women from the state of Pernambuco, Brazil, was performed.

Results:  No significant differences were detected between the hematological profiles of the 2 and 3 trimesters. Nevertheless, the median level of B cells decreased significantly in the 2 (174 × 10 µL;  < 0.002) and 3 trimesters (160 × 10 µL;  < 0.001), compared with the control group (296 × 10 µL). Similarly, the median level of NK cells was lower in the 2 (134 × 10 µL;  < 0.0004) and 3 trimesters (100 × 10 µL,  < 0.0004), compared with the control group (183 × 10 µL). In contrast, relative TCD4+ and TCD8+ levels increased in the 2 and 3 trimesters compared with the controls (TCD4 + : 2 trimester = 59%;  < 0.001; 3 trimester = 57%;  < 0.01; control = 50%; and TCD8 + : 2 trimester = 31%;  < 0.001; 3 trimester = 36%;  < 0.01; control = 24%).

Conclusion:  Low-risk pregnant women have ∼ 40% less B and NK cells in the peripheral blood, compared with non-pregnant women. These parameters may improve health assistance for mothers and contribute to define reference values for normal pregnancies.
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http://dx.doi.org/10.1055/s-0039-1683903DOI Listing
April 2019

High Levels of CXCL8 and Low Levels of CXCL9 and CXCL10 in Women with Maternal RhD Alloimmunization.

Front Immunol 2017 3;8:700. Epub 2017 Jul 3.

Hematology and Hemotherapy Foundation of Pernambuco (HEMOPE), Recife, Brazil.

Maternal RhD alloimmunization is an inflammatory response against protein antigens in fetal red blood cells (RBC). However, not all women become alloimmunized when exposed to RhD fetal RBC. Thus, this study aimed to evaluate levels of inflammatory chemokines in RhD pregnant women with erythrocyte alloimmunization. CXCL8, CXCL9, CCL5, and CXCL10 levels were determined from cell culture supernatants by flow cytometry in 46 (30 non-alloimmunized RhD and 16 previously alloimmunized RhD) pregnant women. CXCL8 levels were significantly higher ( < 0.004), and CXCL9 ( < 0.008) and CXCL10 ( < 0.003) levels were significantly lower in alloimmunized pregnant women. No significant difference in CCL5 levels was detected between the groups. Fetal RHD genotyping was performed in the alloimmunized RhD group by real-time PCR. Anti-D alloantibody was detected in 10 mothers and anti-D and -C in six mothers. Twelve fetuses were RHD positive and four were RHD negative. Further studies of serum chemokines and placenta tissue could provide a better understanding of the cells involved in the pathogenesis of maternal erythrocyte alloimmunization.
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http://dx.doi.org/10.3389/fimmu.2017.00700DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5494496PMC
July 2017
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