Publications by authors named "Juan Zhou"

835 Publications

Association between dietary antioxidant vitamins intake and homocysteine levels in middle-aged and older adults with hypertension: a cross-sectional study.

BMJ Open 2021 Oct 12;11(10):e045732. Epub 2021 Oct 12.

Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China

Objectives: Plasma total homocysteine (tHcy) has been implicated in the development of cardiovascular disease. This study aimed to assess the relationship of dietary antioxidant vitamins intake with tHcy levels in middle-aged and older adults with hypertension.

Design: A cross-sectional study.

Setting: The survey was conducted in the Nanshan district of Shenzhen.

Participants: A total of 1465 middle-aged and older adults with hypertension were included between July and September of 2013.

Measurements: Hyperhomocysteinaemia (HHcy) was defined as tHcy ≥15 µmol/L. Some dietary antioxidant vitamins (vitamin C (VC) and vitamin E (VE), carotenes, retinol, lutein) intake was estimated using the Food Frequency Questionnaire. Sociodemographic and potential covariates were evaluated through questionnaires, anthropometric measurements and blood tests. The association between dietary intakes of antioxidant vitamins and tHcy concentration were evaluated by multiple linear regression analyses after napierian logarithm transformed. Multiple logistic regression models were further used to determine ORs and 95% CIs.

Results: The β (95% CIs) of VC intake and tHcy was -0.050 (-0.084 to -0.016). Compared with the lowest quartile in the fully adjusted model, the ORs (95% CIs) for HHcy levels across quartiles of dietary VC intake were 0.82 (0.57 to 1.16), 0.49 (0.33 to 0.74) and 0.40 (0.22 to 0.74) (p for trend=0.001). The β (95% CIs) of retinol intake and tHcy was -0.021 (-0.041 to -0.002) and the ORs (95% CIs) in the third quartile of retinol intake was 0.61 (0.42 to 0.86), while the effect for the highest quartile was not significant (p for trend=0.951). No significant association was observed between dietary VE, carotenes and lutein intake and HHcy.

Conclusions: A linear inverse association between dietary VC intake and HHcy prevalence, and an L-shaped association between dietary retinol intake and HHcy prevalence were found in Chinese middle-aged and older adults with hypertension.
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http://dx.doi.org/10.1136/bmjopen-2020-045732DOI Listing
October 2021

Deregulated Expression of miR-483-3p Serves as a Diagnostic Biomarker in Severe Pneumonia Children with Respiratory Failure and Its Predictive Value for the Clinical Outcome of Patients.

Mol Biotechnol 2021 Oct 12. Epub 2021 Oct 12.

Department of Neurology, Yidu Central Hospital of Weifang, 4138 Linglongshan Road, Qingzhou, Weifang, 262500, Shandong, China.

Severe pneumonia in children is a group of inflammatory diseases of respiratory tract caused by pathogenic microorganisms. Increasing evidence suggested the crucial effects of microRNA on inflammatory diseases. This study aimed to reveal the expression and role of miR-483-3p in the serum of children with severe pneumonia, and to explore the effect of miR-483-3p on the biological function of lipopolysaccharide (LPS)-induced MRC-5 cells. MRC-5 cells were disposed with LPS to construct an in vitro pneumonia cell model. The relative expression level of miR-483-3p was measured by qRT-PCR. ROC curve was used to evaluate the diagnostic value of miR-483-3p in severe pneumonia. The Kaplan-Meier curve was performed to test the characteristics of survival distribution of different miRNA classifications. Cell viability and apoptosis were performed by CCK-8 assay and flow cytometry. IL-1β, TNF-α, and IL-6 were detected by ELISA. Luciferase reporter gene assay and western blot analysis were performed to detect the interaction between miR-483-3p and IGF-1. The expression of serum miR-483-3p in severe pneumonia patients was higher than in controls. The AUC value of the ROC curve was 0.919, indicating that miR-483-3p had diagnostic value for severe pneumonia. The survival curve showed that patients with high expression of miR-483-3p had higher mortality. Cell viability and apoptosis assay showed that overexpression of miR-483-3p suppressed cell proliferation and promoted apoptosis. And upregulation of miR-483-3p promoted generation of inflammatory cytokines. Luciferase report gene assay and western blot assay both illustrated that IGF-1 might be the target gene of miR-483-3p. Serum miR-483-3p can be used as a biomarker for the diagnosis of severe pneumonia. High expression of miR-483-3p promoted the development of severe pneumonia.
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http://dx.doi.org/10.1007/s12033-021-00415-7DOI Listing
October 2021

Lymphocyte subsets as a predictor of severity and prognosis in COVID-19 patients.

Int J Immunopathol Pharmacol 2021 Jan-Dec;35:20587384211048567

Department of Infectious Disease, Huoshenshan Hospital, Wuhan, China.

Background: Coronavirus disease 2019 (COVID-19) had become a worldwide health threat. Early prediction of the severity of COVID-19 patients was important for reducing death rate and controlling this disease.

Methods And Materials: A total of 301 patients confirmed with COVID-19 in Wuhan from 8 February to 10 April 2020 were included. Clinical data were collected and analyzed. Diagnostic and prognostic utility of blood cell counts and lymphocyte subsets in COVID-19 patients were investigated. The receiver operator characteristic curve (ROC) was used in discriminating the mild and severe/critical cases.

Results: There were difference in blood cell counts and lymphocyte subsets among mild, severe and critical patients, which were also influenced by comorbidities and duration of disease. The area under the ROC of lymphocyte, CD3 T cells, CD4 T cells, and CD8 T cells were 0.718, 0.721, 0.718, and 0.670, which were higher than that of other hematological parameters. The optimal threshold was 1205, 691, 402, and 177 per μl, respectively. Patients with higher counts of lymphocyte, CD3 T cells, CD4 T cells, or CD8 T cells were correlated with shorter length of stay in hospital ( < 0.05). Multivariable Cox regression analysis showed disease severity, CD3 T cells counts and time when the nucleic acid turned negative were independent risk factors for in-hospital death of COVID-19 patients ( < 0.05).

Conclusion: Blood cell counts and lymphocyte subsets correlated with severity of COVID-19.
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http://dx.doi.org/10.1177/20587384211048567DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8504275PMC
October 2021

Unveiling the role of sediments in phosphorus removal in pilot-scale constructed wetlands for swine wastewater treatment.

Sci Total Environ 2021 Oct 2;807(Pt 1):150684. Epub 2021 Oct 2.

Key Laboratory of Agro-ecological Processes in Subtropical Regions, Changsha Research Station for Agricultural & Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; University of Chinese Academy of Sciences, Beijing 100049, China.

The accumulation rate, fractions, and sorption capacity of phosphorus in sediments determine the removal efficiency and service life of constructed wetlands (CWs). Nine pilot-scale three-stage surface flow CWs were constructed to treat three loading rates of lagoon-pretreated swine wastewater, and surface sediment samples at initial and one-year treatment were collected to analyze the phosphorus fractions and sorption capacity. After one-year treatment, concentration of total phosphorus (TP) in sediments increased for high loading rates of wastewater, but remained stable for low loading rates. The annual accumulation rate of TP in sediments (M) was -43-445 mg kg yr at surface loading rate (SLR) of 36-355 g P m yr. Their association could be described well using a sigmoid model, i.e., M = -23 + 538/(1 + exp.(-(SLR-262)/48)) (R = 0.897, RMSE = 40.8, p < 0.01), indicating that the phosphorus accumulation rates in sediments were loading rate-dependent. The sum of inorganic phosphorus fractions contributed to 80-100% of the TP concentration, and accumulation of aluminum-bound phosphorus (AlP) and iron-bound phosphorus (FeP) was responsible for variability of TP concentration in sediments. Phosphorus sorption capacity of CW1 sediments increased by 1.3-1.8 times, attributed to increased pH, and concentrations of ammonium oxalate-extractable aluminum and iron in sediments due to the wastewater input. Selecting iron and aluminum-rich materials preferentially as substrates and regulating the ratio of metal ions to phosphorus in wastewater should be alternative enhancement strategies of CWs for phosphorus removal.
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http://dx.doi.org/10.1016/j.scitotenv.2021.150684DOI Listing
October 2021

Bilingual language entropy influences executive functions through functional connectivity and signal variability.

Brain Lang 2021 Nov 28;222:105026. Epub 2021 Sep 28.

Humanities, Arts and Social Sciences, Singapore University of Technology and Design, Singapore. Electronic address:

How bilinguals' experience in managing multiple languages in different communicative contexts influences the cognitive and neural aspects of executive functions remains unclear. Therefore, we examined whether variations in language experience in young adult bilinguals were associated with data-driven brain functional network patterns (connectivity and signal variability) defined by performance during executive control tasks (Stroop and task-switching). Multiple aspects of language experience, such as the extent of balanced bilingualism in language proficiency and usage, and language diversity across social contexts (i.e., language entropy) were assessed. We found that greater language diversity, rather than balanced bilingualism per se, was related to higher brain network specialization and segregation concentrated on the default mode and executive control networks, and lower signal variability, a pattern linked to smaller RT-indices related to executive functioning of shifting, goal-maintenance, and conflict-monitoring. Our findings underscore the important role of language diversity in influencing bilinguals' neurocognitive characteristics of executive functioning.
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http://dx.doi.org/10.1016/j.bandl.2021.105026DOI Listing
November 2021

Multi-Modal Imaging Probe for Glypican-3 Overexpressed in Orthotopic Hepatocellular Carcinoma.

J Med Chem 2021 Sep 30. Epub 2021 Sep 30.

Department of Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor, Michigan 48109, United States.

Hepatocellular carcinoma (HCC) is rising steadily in incidence, and more effective methods are needed for early detection and image-guided surgery. Glypican-3 (GPC3) is a cell surface biomarker that is overexpressed in early-stage cancer but not in cirrhosis. An IRDye800-labeled 12-mer amino acid sequence was identified, and specific binding to GPC3 was validated and in orthotopically implanted HCC tumors . Over 4-fold greater binding affinity and 2-fold faster kinetics were measured by comparison with previous GPC3 peptides. Photoacoustic images showed peak tumor uptake at 1.5 h post-injection and clearance within ∼24 h. Laparoscopic and whole-body fluorescence images showed strong intensity from tumor versus adjacent liver with about a 2-fold increase. Immunofluorescence staining of human liver specimens demonstrated specific binding to HCC versus cirrhosis with 79% sensitivity and 79% specificity, and normal liver with 81% sensitivity and 84% specificity. The near-infrared peptide is promising for early HCC detection in clinical trials.
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http://dx.doi.org/10.1021/acs.jmedchem.1c00697DOI Listing
September 2021

Fabrication of pH/Reduction Sensitive Polyethylene Glycol-Based Micelles for Enhanced Intracellular Drug Release.

Pharmaceutics 2021 Sep 14;13(9). Epub 2021 Sep 14.

Department of Pharmaceutics, School of Pharmaceutical Sciences, Jiangnan University, Wuxi 214122, China.

At present, the drug is still difficult to release completely and quickly only with single stimulation. In order to promote the rapid release of polymeric micelles at tumor site, pH/reduction sensitive polymers (PCT) containing disulfide bonds and orthoester groups were synthesized. The PCT polymers can self-assemble in water and entrap doxorubicin to form drug-loaded micelles (DOX/PCT). In an in vitro drug release experiment, the cumulative release of DOX/PCT micelles in the simulated tumor microenvironment (pH 5.0 with GSH) reached (89.7 ± 11.7)% at 72 h, while it was only (16.7 ± 6.1)% in the normal physiological environment (pH 7.4 without GSH). In addition, pH sensitive DOX loaded micellar system (DOX/PAT) was prepared as a control. Furthermore, compared with DOX/PAT micelles, DOX/PCT micelles showed the stronger cytotoxicity against tumor cells to achieve an effective antitumor effect. After being internalized by clathrin/caveolin-mediated endocytosis and macropinocytosis, DOX/PCT micelles were depolymerized in intercellular acidic and a reductive environment to release DOX rapidly to kill tumor cells. Additionally, DOX/PCT micelles had a better inhibitory effect on tumor growth than DOX/PAT micelles in in vivo antitumor activity studies. Therefore, pH/reduction dual sensitive PCT polymers have great potential to be used as repaid release nanocarriers for intercellular delivery of antitumor drugs.
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http://dx.doi.org/10.3390/pharmaceutics13091464DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470983PMC
September 2021

Second-generation sequencing assistance in the diagnosis of empyema: A case report.

Zhong Nan Da Xue Xue Bao Yi Xue Ban 2021 Aug;46(8):920-924

First Division, Department of Respiratory and Critical Care Medicine, Zhuzhou Hospital, Affiliated to Xiangya School of Medicine, Central South University/Zhuzhou Central Hospital, Zhuzhou Hunan 412007, China.

empyema is rare and easy to be misdiagnosed. An 81-year-old male patient showed symptoms with cough, sputum, and fever for 3 days. Community-acquired pneumonia was diagnosed firstly. After anti-infection treatment, the patient was still in fever. Chest radiography showed pleural effusion, closed thoracic drainage was performed and the reddish-brown fluid was drained out. The second-generation sequencing was performed on pleural fluid and was detected. has strict requirements for growth conditions and it difficult to cultivate. The application of second-generation sequencing is helpful to diagnose the pathogen rapidly.
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http://dx.doi.org/10.11817/j.issn.1672-7347.2021.200589DOI Listing
August 2021

Dramatically comprehensive improved electrochemical performances of symmetric and asymmetric supercapacitors under external magnetic field.

Chem Commun (Camb) 2021 Sep 14;57(73):9216-9219. Epub 2021 Sep 14.

Jilin University, School of Materials Science and Engineering, Changchun, Jilin, China.

Recently, magnetic field (MF) has been described as a potential way to improve the properties of electrode materials, which significantly enhances the ion diffusion behavior and material wettability. We first synthezised for the first time a comparable carbon-based electrode material (GCA) including active carbon, reduced graphene and carbon nanotubes through a facile stirring method for further research under MF. Herein, 0.15 T of MF induced by two NdFeB magnets was applied on the supercapacitor devices to enhance the energy density, which increased by about 62% for the symmetric supercapacitor (SSC: from 11.2 to 18.1 W h kg). An asymmetric supercapacitor composed of the prepared GCA as the anode and NiCoFe/NiCoFe-OH as the cathode was also assembled for research. And it was found that the whole electrochemical performance significantly improved (for example, energy density increased by about 22% for the asymmetric supercapacitor, , from 50.6 to 61.4 W h kg).
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http://dx.doi.org/10.1039/d1cc03289eDOI Listing
September 2021

Ascorbic Acid Inhibits Liver Cancer Growth and Metastasis and , Independent of Stemness Gene Regulation.

Front Pharmacol 2021 24;12:726015. Epub 2021 Aug 24.

School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.

Experimental and clinical evidence has indicated that the natural product ascorbic acid (AA) is effective in preventing and treating various types of cancers. However, the effect of AA on liver cancer metastasis has not yet been reported. Cancer stem cells (CSCs) play pivotal roles in cancer metastasis. Here, we demonstrated that AA selectively inhibited the viability of both liver cancer cells and CSCs, reduced the formation of cancer cell colonies and CSC spheres, and inhibited tumor growth . Additionally, AA prevented liver cancer metastasis in a xenotransplantation model without suppressing stemness gene expression in liver CSCs. Further study indicated that AA increased the concentration of HO and induced apoptosis in liver CSCs. Catalase attenuated the inhibitory effects of AA on liver CSC viability. In conclusion, AA inhibited the viability of liver CSCs and the growth and metastasis of liver cancer cells and by increasing the production of HO and inducing apoptosis. Our findings provide evidence that AA exerts its anti-liver cancer efficacy and , in a manner that is independent of stemness gene regulation.
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http://dx.doi.org/10.3389/fphar.2021.726015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8422961PMC
August 2021

Efficacy of different nucleoside analog rescue therapies for entecavir-resistant chronic hepatitis B patients.

BMC Infect Dis 2021 Sep 6;21(1):912. Epub 2021 Sep 6.

Center of Infectious Diseases, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Wuhou District, Chengdu, 610041, Sichuan, China.

Background: Entecavir (ETV) is recommended as a first-line anti-HBV treatment. However, many chronic hepatitis B patients initiate anti-HBV treatment such as lamivudine and telbivudine with low genetic barriers in China, which leads to compensatory mutations and increases the rate of ETV resistance. The management of ETV resistance in China is an essential clinical issue.

Methods: Patients from 2011 to 2017 with nucleos(t)ide analog resistance were screened and 72 patients with ETV resistance were included. These patients received different rescue therapies including an ETV and adefovir (ADV) combination therapy group (n = 25), a tenofovir (TDF) monotherapy group (n = 27), and an ETV and TDF combination therapy group (n = 20). Virologic, biochemical, and serologic responses were compared among the three groups.

Results: The rate of ETV resistance among all HBV-resistant variants increased from 6.04% in 2011 to 15.02% in 2017. TDF monotherapy and TDF combination groups showed similar rates of negative HBV DNA at 48 weeks (74.07% vs 70.00%, P > 0.05), while the ETV and ADV group showed the worst virologic response (28.00%). Also, TDF monotherapy and TDF combination therapy showed similar decline of HBV DNA at weeks 12, 24, and 48. There was no significant difference in the rates of HBeAg clearance, ALT normalization, and abnormal renal function among the three groups.

Conclusions: TDF monotherapy showed a comparable virologic response to TDF and ETV combination therapy and a better virologic response than ETV and ADV combination therapy. Thus, TDF monotherapy is the preferred rescue therapy for ETV resistance.
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http://dx.doi.org/10.1186/s12879-021-06554-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8420064PMC
September 2021

Utility of intestinal intravital microscopy for the study of CNS injury-induced immunodepression syndrome (CIDS).

Clin Hemorheol Microcirc 2021 ;79(1):137-147

Department of Pharmacology, Dalhousie University, Halifax, NS, Canada.

Background: Stroke, traumatic brain injury, or other forms of central nervous system (CNS) injury initiate a local inflammatory response. Compensatory anti-inflammatory pathways are activated to limit secondary damage due to inflammation. The associated release of immunosuppressing neuromodulators can result in system-wide immune dysregulation (CNS injury-induced immune-depression syndrome -CIDS).

Objective: To establish an experimental stroke model where CIDS can be studied by intravital microscopy (IVM).

Methods: We used the photothrombotic stroke (PTS) model in C57BL/6 mice and studied its effects on peripheral immunity following challenge with lipopolysaccharide (LPS). Leukocyte activation, as well as capillary perfusion of the microcirculation, were assessed using intestinal intravital microscopy (IVM).

Results: PTS caused a significant reduction in the number of adhering leukocytes in submucosal venules of the terminal ileum of mice challenged with LPS compared to LPS-challenged animals without stroke. Leukocyte rolling was also impacted by PTS in the submucosal venules. Following stroke, we also observed decreased mucosal functional capillary density (FCD).

Conclusions: Our results suggest that PTS with subsequent LPS challenge poses as a viable model to further study CIDS using intravital microscopy of the intestinal microcirculation.
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http://dx.doi.org/10.3233/CH-219109DOI Listing
January 2021

Evidence-Based Analysis on Observation for Nursing Care of Patients with Intra-Aortic Balloon Pumping.

Evid Based Complement Alternat Med 2021 26;2021:5954343. Epub 2021 Aug 26.

Department of Hematology, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha 410005, China.

In this study, data analysis was performed on 52 patients. According to the different outcomes and discharge diagnosis of patients, data on sedative use, emotions, behavioral abnormalities, hearing loss, pain, total time on board the IABP (intra-aortic balloon pumping), and days of hospitalization of patients were collected. The data were subjected to frequency analysis, paired chi-square analysis, chi-square test, Poisson regression analysis, and stepwise regression analysis. Some findings of the analysis included the following: Between outcome and admission diagnosis, the analysis showed that significant differences existed between paired data. Patients with heart failure and acute myocardial infarction are in an unhealed state, and most patients with coronary atherosclerotic heart disease, myocarditis, and heart disease showed improvement. The samples taken by different sedatives showed no significant differences in the emotional and behavioral abnormalities, hearing loss, and pain. A total of 1 item of hospital stay had a significant negative impact on the total operation time of IABP. However, discharge diagnosis and admission diagnosis did not affect the total time on board the IABP. The dorsalis pedis artery pulse condition has a significant negative effect on the total time on board the IABP.
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http://dx.doi.org/10.1155/2021/5954343DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8416337PMC
August 2021

Bifunctional magnetic covalent organic framework for simultaneous enrichment of phosphopeptides and glycopeptides.

Anal Chim Acta 2021 Sep 19;1177:338761. Epub 2021 Jun 19.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, PR China. Electronic address:

Protein phosphorylation and glycosylation, which are closely related to various diseases, have been extensively studied recently. Mass spectrometry (MS) based phosphoproteomics and glycoproteomics analysis rely heavily on the pre-treatment. Due to the differences in enrichment conditions, there are still huge challenges in designing and preparing a single affinity material to achieve efficient simultaneous capture and elution of phosphopeptides and glycopeptides. Herein, a novel magnetic covalent organic framework, which was modified with functional molecule 4-(3-(2-(methacryloyloxy)ethyl)-ureido)benzoic acid (MUBA), was designed as a bifunctional enrichment platform for glycopeptides and phosphopeptides. Thanks to the multiple hydrogen bonding interactions between MUBA and hydrogen phosphates, the material possessed excellent enrichment performance for phosphopeptides. In addition, the hydrophilicity of the COF structure and modified molecules endowed this material recognition capability towards glycopeptides based on hydrophilic interaction chromatography. Combining with the inherent properties of COF structure, the established platform achieved simultaneous enrichment of phosphopeptides and glycopeptides with excellent selectivity (1:1:1000 M ratio of α-casein/IgG/BSA), high sensitivity (0.05 fmol/μL α-casein; 0.05 fmol/μL IgG), and good size-exclusion effect (α-casein digests/IgG digests/BSA, 1:1:500). More excitingly, the method was used for the identification of glycopeptides and phosphopeptides from rat liver tissue and the exosomes extracted from liver cancer patients' plasma, proving its specific phosphoproteomics and glycoproteomics study in complex biosamples.
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http://dx.doi.org/10.1016/j.aca.2021.338761DOI Listing
September 2021

Probing Multidimensional Structural Information of Single Molecules Transporting through a Sub-10 nm Conical Plasmonic Nanopore by SERS.

Anal Chem 2021 08 20;93(34):11679-11685. Epub 2021 Aug 20.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Probing the orientation and oxygenation state of single molecules (SMs) is of great importance for understanding the advanced structure of individual molecules. Here, we manipulate molecules transporting through the hot spot of a sub-10 nm conical gold nanopore and acquire the multidimensional structural information of the SMs by surface enhanced Raman scattering (SERS) detection. The sub-10 nm size and conical shape of the plasmonic nanopore guarantee its high detection sensitivity. SERS spectra show a high correlation with the orientations of small-sized single rhodamine 6G (R6G) during transport. Meanwhile, SERS spectra of a single hemoglobin (Hb) reveal both the vertical/parallel orientations of the porphyrin ring and oxygenated/deoxygenated states of Hb. The present study provides a new strategy for bridging the primary sequence and the advanced structure of SMs.
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http://dx.doi.org/10.1021/acs.analchem.1c00875DOI Listing
August 2021

Identification of sp. nov. and sp. nov., two novel species of the family isolated from faeces of bats (.).

Int J Syst Evol Microbiol 2021 Aug;71(8)

Department of Epidemiology, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, Jiangsu Province, PR China.

Two pairs of aerobic, Gram-stain-positive, rod-shaped strains (HY164/HY044, HY168/HY211) were isolated from bat faecal samples. Strains HY164 and HY044 were motile with a polar flagellum, and had 16S rRNA gene similarity of 95.1-98.6 % to YIM 93306 and T3246-1; strains HY168 and HY211 were most similar to DSM 18029 (96.6 %). Phylogenetic trees based on 16S rRNA gene and whole genome sequences revealed affiliation of strains HY164 and HY168 to the family , representing novel lineages in the genera and , respectively, which was also supported by the results for average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH). For all isolates, the principal cellular fatty acids were -C and -C. HY164 and HY168 had MK-8(H) as the predominant isoprenoid quinone, diphosphatidylglycerol, phosphatidylglycerol, several unidentified phospholipids and glycolipids as common polar lipids while the latter strain additionally contained one unidentified aminophospholipid and one unidentified phosphoglycolipid. Besides sharing alanine, glutamic acid and lysine with HY164, HY168 additionally contained 2,4-diaminobutyric acid in the cell-wall peptidoglycan. The whole-cell sugars of HY164 were ribose and rhamnose, while HY168 only included the latter. The DNA G+C contents of HY164 and HY168 were 71.0 and 69.1 mol%, respectively. Combining the polyphasic taxonomic data, HY164 (=CGMCC 4.7606=JCM 33464) is classified as representing a novel species of the genus with the proposed name sp. nov., and HY168 (=CGMCC 1.16970=JCM 33465) is proposed to represent a novel species of the genus with the name sp. nov.
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http://dx.doi.org/10.1099/ijsem.0.004953DOI Listing
August 2021

A case-control study on correlation between the single nucleotide polymorphism of CLEC4E and the susceptibility to tuberculosis among Han people in Western China.

BMC Infect Dis 2021 Aug 10;21(1):788. Epub 2021 Aug 10.

Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China.

Background: Tuberculosis (TB) is one of the leading causes of morbidity and mortality in Western China. Preclinical studies have suggested the protective effect of the C-type lectin receptor of family 4 member E (CLEC4E) from TB. Herein, we investigated the association between CLEC4E gene variants and TB susceptibility in a western Chinese Han population.

Methods: We genotyped four single nucleotide polymorphisms (SNPs) rs10841856, rs10770847, rs10770855 and rs4480590 in the CLEC4E gene using the improved multiplex ligation detection reaction (iMLDR) assay in 900 TB cases and 1534 healthy controls.

Results: After stratifying the whole data by sex, it was found that males exhibited mutant allele G of rs10841856 was more strongly associated with increased TB risk after Bonferroni correction (OR = 1.334, 95% CI: 1.142-1.560; P < 0.001 after adjusting for age; p = 0.001 after Bonferroni correction). The genetic model analysis found that rs10841856 was associated with the increased risk of TB among males under the dominant model (OR = 1.557, 95% CI = 1.228-1.984, P < 0.001 after adjusting for age, P < 0.001 after Bonferroni correction). Bioinformatics analysis suggested that rs10841856 might fall in putative functional regions and might be the expression quantitative trait loci (eQTL) for CLEC4E and long noncoding RNA RP11-561P12.5.

Conclusions: Our study revealed that rs10841856 in the CLEC4E gene might be related to increased TB risk, especially the dominant genetic model among male Han individuals from Western China.
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http://dx.doi.org/10.1186/s12879-021-06448-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353747PMC
August 2021

Interactive effects of serum ferritin and high sensitivity C-reactive protein on diabetes in hypertensive patients.

J Trace Elem Med Biol 2021 Jul 28;68:126824. Epub 2021 Jul 28.

Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, and Ministry of Education (MOE) Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, 430030, PR China. Electronic address:

Background: Hypertensive patients, often characterized by chronic inflammation, are susceptible to diabetes. Evidence suggests that the positive association between serum ferritin (SF) and diabetes was affected by high-sensitivity C-reactive protein (hs-CRP), an inflammation marker. We investigate whether there was an interaction between SF and hs-CRP on diabetes in hypertensive patients.

Methods: We analysed data of 1,735 hypertensive people in this cross-sectional study. Diabetes was diagnosed when fasting blood glucose ≥ 7.0 mmol/L and/or a previous clinical diagnosis of diabetes. Logistic regression models were used to estimate the association of the SF and hs-CRP with diabetes. Multiplicative interaction was evaluated by incorporating a cross-product term for SF and hs-CRP to the logistic regression model. Additive interaction was assessed by calculating the relative excess risk of interaction (RERI) and attributed proportion due to interaction (AP).

Results: In the adjusted analysis, SF (highest vs lowest tertile: odds ratio [OR], 1.61; 95 % confidence interval [CI], 1.20-2.16) was positively associated with diabetes. There was no multiplicative interaction between SF and hs-CRP, but evidence of additive interaction in regard to diabetes (RERI: 0.86; 95 % CI: 0.06-1.67). Compared to the patients with low SF (lower two thirds) and low hs-CRP (≤ 2 mg/L), those with high SF (upper one third) and high hs-CRP (> 2 mg/L) had increased OR for diabetes (adjusted OR: 2.33 [1.65-3.30]), with 37.0 % of the effects attributed to the additive interaction (AP: 0.37; 95 % CI: 0.09-0.65).

Conclusions: Within a cross-sectional study consisting of hypertensive patients, co-exposure to high SF and high hs-CRP was synergistically associated with diabetes. Dietary intervention or pharmacological treatment to lowering SF concentration may help to reduce diabetes morbidity in hypertensive patient with chronic inflammation.
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http://dx.doi.org/10.1016/j.jtemb.2021.126824DOI Listing
July 2021

LncRNA CASC2 inhibits lung adenocarcinoma progression through forming feedback loop with miR-21/p53 axis.

Kaohsiung J Med Sci 2021 Aug 1;37(8):675-685. Epub 2021 Aug 1.

Department of Oncology, Zhuzhou Central Hospital, Zhuzhou, People's Republic of China.

Lung adenocarcinoma (LUAD) is the most common type of lung cancer. Currently, the survival rate of LUAD patients remains low due to heterogeneity and high invasiveness. The long non-coding RNA (lncRNA) cancer susceptibility candidate 2 (CASC2) is reported to be related to LUAD development. Hence, we investigate the roles and regulatory mechanism of CASC2 in LUAD. The expression levels of CASC2, microRNA (miR)-21, and p53 were quantified by quantitative real-time polymerase chain reaction, and the protein levels of Bax, Bcl-2, p53, and p21 were examined using western blotting. A dual-luciferase reporter experiment was conducted to prove the molecular interactions between CASC2 and miR-21 or p53. CCK-8 and flow cytometry assays were conducted to assess cell proliferation and apoptosis, respectively. CASC2 was expressed at a low level in LUAD patients and LUAD cell lines. CASC2 overexpression markedly suppressed cell proliferation and enhanced apoptosis. Mechanistically, CASC2 overexpression dramatically inhibited miR-21 expression and increased p53 expression by directly targeting miR-21. Moreover, rescue experiments suggested that either miR-21 overexpression or p53 silencing obviously weakened the biological effects of CASC2 overexpression. In addition, p53 was proven to be an upstream transcription factor of CASC2 and can activate CASC2 transcription. These results provide evidence that the lncRNA CASC2/miR-21/p53 form a positive feedback loop to mediate cell proliferation and apoptosis in LUAD, which may provide a new insight into the pathological mechanisms of LUAD.
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http://dx.doi.org/10.1002/kjm2.12386DOI Listing
August 2021

Brain Functional Changes in Stroke Following Rehabilitation Using Brain-Computer Interface-Assisted Motor Imagery With and Without tDCS: A Pilot Study.

Front Hum Neurosci 2021 16;15:692304. Epub 2021 Jul 16.

Center for Sleep and Cognition, Center for Translational MR Research, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.

Brain-computer interface-assisted motor imagery (MI-BCI) or transcranial direct current stimulation (tDCS) has been proven effective in post-stroke motor function enhancement, yet whether the combination of MI-BCI and tDCS may further benefit the rehabilitation of motor functions remains unknown. This study investigated brain functional activity and connectivity changes after a 2 week MI-BCI and tDCS combined intervention in 19 chronic subcortical stroke patients. Patients were randomized into MI-BCI with tDCS group and MI-BCI only group who underwent 10 sessions of 20 min real or sham tDCS followed by 1 h MI-BCI training with robotic feedback. We derived amplitude of low-frequency fluctuation (ALFF), regional homogeneity (ReHo), and functional connectivity (FC) from resting-state functional magnetic resonance imaging (fMRI) data pre- and post-intervention. At baseline, stroke patients had lower ALFF in the ipsilesional somatomotor network (SMN), lower ReHo in the contralesional insula, and higher ALFF/Reho in the bilateral posterior default mode network (DMN) compared to age-matched healthy controls. After the intervention, the MI-BCI only group showed increased ALFF in contralesional SMN and decreased ALFF/Reho in the posterior DMN. In contrast, no post-intervention changes were detected in the MI-BCI + tDCS group. Furthermore, higher increases in ALFF/ReHo/FC measures were related to better motor function recovery (measured by the Fugl-Meyer Assessment scores) in the MI-BCI group while the opposite association was detected in the MI-BCI + tDCS group. Taken together, our findings suggest that brain functional re-normalization and network-specific compensation were found in the MI-BCI only group but not in the MI-BCI + tDCS group although both groups gained significant motor function improvement post-intervention with no group difference. MI-BCI and tDCS may exert differential or even opposing impact on brain functional reorganization during post-stroke motor rehabilitation; therefore, the integration of the two strategies requires further refinement to improve efficacy and effectiveness.
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http://dx.doi.org/10.3389/fnhum.2021.692304DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8322606PMC
July 2021

Urine-based regenerative RNA biomarkers for urinary bladder wound healing.

Regen Med 2021 08 2;16(8):709-718. Epub 2021 Aug 2.

GuangxiMedical University Cancer Hospital & Guangxi Cancer Research Institute, Nanning, 530021, PR China.

This study aimed to investigate the expression of regeneration-related genes in canine urine during bladder repair. Canine urine samples were collected after partial cystectomy. Regenerative mRNA of hypoxia-inducible factor (HIF), vascular endothelial growth factor (VEGF), key stem cell transcription factors and cholinergic signals were detected. HIF-1α, VEGF, CD44, IL-6 and prominin-1 expression in canine urine after partial cystectomy exhibited two similar peaks at ∼2 weeks. HIF-1α and VEGF expression were higher in the afternoon than the morning. The expression of key stem cell transcription factors and cholinergic signals also exhibited a rhythm along with bladder healing. The expression of HIF-1α, VEGF, key stem cell transcription factors and cholinergic signals exhibited a time curve distribution during canine bladder healing. The expression trend of some regenerative genes was similar during bladder healing, and a cooperative effect may exist.
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http://dx.doi.org/10.2217/rme-2021-0057DOI Listing
August 2021

Relationship between Lp-PLA2 and in-stent restenosis after coronary stenting: a 3-year follow-up study.

Scott Med J 2021 Jul 28:369330211034809. Epub 2021 Jul 28.

Chief Physician, Department of Cardiology, Haimen Hospital of Nantong University, China.

Background And Aims: Coronary in-stent restenosis (ISR) is an important complication of percutaneous coronary intervention (PCI). However, the relationship between lipoprotein associated phospholipase A2 (Lp-PLA2) level and ISR after PCI is rarely reported. This study aims to explore the relationship between Lp-PLA2 and the occurrence of ISR at post-PCI and its predictive value for ISR.

Methods And Results: Plasma Lp-PLA2 mass were measured in 847 patients planting 1262 stents and evaluated along with known risk indicators. One-year angiographic follow-up showed that baseline elevated Lp-PLA2 mass was strongly associated with early restenosis (95% CI = 1.062-3.050, P < 0.05). Beyond the first year, the occurrence of late restenosis (95% CI = 1.043-3.214, P < 0.05) was significantly larger in the elevated Lp-PLA2 group. Kaplan-Meier analysis after three-year clinical follow up suggested that Lp-PLA2 mass did add the positive effect on the occurrence of major adverse cardiovascular events (MACEs).

Conclusion: In conclusion, increased baseline plasma Lp-PLA2 predicts increased risks of re-stenosis and MACEs, which may be a novel biomarker for predicting ISR and MACEs.
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http://dx.doi.org/10.1177/00369330211034809DOI Listing
July 2021

Construction of a magnetic covalent organic framework with synergistic affinity strategy for enhanced glycopeptide enrichment.

J Mater Chem B 2021 Aug 23;9(32):6377-6386. Epub 2021 Jul 23.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, P. R. China.

Considering the inherent properties of glycopeptides, such as glycan structure, size, and hydrophilicity, affinity materials possessing suitable functional molecule-glycan interactions, matched channels with size exclusion, and surfaces with hydrophilic interactions are preferred for glycopeptide separation in biological samples. Here, a novel boronic-acid-functionalized magnetic covalent organic framework was prepared through epitaxial growth and multi-ligand strategies. The multi-ligand strategy was firstly employed to prepare functionalized magnetic covalent organic framework without any post-functionalization protocol. Notably, the proposed strategy was found to be time saving, robust, and reproducible. The versatile magnetic covalent organic framework nanocomposite was endowed with phenylboronic acid functional molecules, strong hydrophilic features, mesoporous channels, fast magnetic responsiveness, and a large surface area. Benefitting from multiple affinity interactions, namely, synergistic reversible covalent interactions and hydrophilic affinity interactions, the nanocomposite presented extremely high performance in the recognition of intact N-glycopeptides. The inherent properties endowed the nanocomposite with excellent enrichment performance for N-glycopeptides: excellent selectivity (1 : 2000, IgG/BSA, m/m), an ultralow detection limit (0.05 fmol μL), and a good size-exclusion effect (1 : 500, IgG digests/BSA, m/m). More excitingly, a total of 1921 unique intact glycopeptides assigned to 1154 glycoproteins were identified from rat liver tissue; this performance is superior to that of commercial products. Additionally, the nanocomposite was successfully applied to enrich intact glycopeptides of exosomes extracted from healthy individuals and renal failure patients, providing a novel concept for the design of materials using a synergistic affinity strategy for sample preparation in glycoproteomics.
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http://dx.doi.org/10.1039/d1tb01168eDOI Listing
August 2021

Correction: A light-up fluorescence resonance energy transfer magnetic aptamer-sensor for ultra-sensitive lung cancer exosome detection.

J Mater Chem B 2021 Jul;9(29):5914

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, P. R. China.

Correction for 'A light-up fluorescence resonance energy transfer magnetic aptamer-sensor for ultra-sensitive lung cancer exosome detection' by Nanhang Zhu et al., J. Mater. Chem. B, 2021, 9, 2483-2493, DOI: 10.1039/D1TB00046B.
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http://dx.doi.org/10.1039/d1tb90039kDOI Listing
July 2021

Establishing a patient registry study database of dust mite allergic asthma in children: design, methodology and preliminary exploration.

Ann Transl Med 2021 Jun;9(12):993

Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing, China.

Background: Asthma is a heterogeneous disease with different phenotypes, endotypes and responses to treatment. Dust mite allergic asthma (DMAA) is the most common type in children. Compared with randomized control trials, a patient registry study (PRS) can reflect the real physical condition and clinical diagnosis more comprehensively.

Methods: Children who visited the asthma clinic of the Children's Hospital of Chongqing Medical University between August 2018 and August 2020, and met the inclusion criteria and also agreed to participate, were enrolled in the registry study. Clinical information, laboratory tests and peripheral blood samples were collected after informed consent was given by guardians.

Results: To date, 208 children have been enrolled in the patient registry database of DMAA. They are mainly male, with >50% having a history of allergic rhinitis, cesarean section, positive family history and passive smoking. Eosinophils and total immunoglobulin E levels were all significantly higher than normal. According to results for the childhood asthma control test (c-ACT) and ratio of forced expiratory volume in 1 s to predicted value after inhaled corticosteroid treatment, the uncontrolled group had higher hemoglobin (Hb) levels than the control group. The group exhibiting abnormal pulmonary function was older, and had longer disease duration, higher fractional exhaled nitric oxide and Hb than the group in which pulmonary function was restored.

Conclusions: We have preliminarily established a registered study database of children with DMAA. By cluster analysis and using blood samples, we can further study the different pathophysiological mechanisms in order to provide more individualized and targeted treatments for all children.
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http://dx.doi.org/10.21037/atm-21-2566DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267296PMC
June 2021

Injectable, self-healing and pH responsive stem cell factor loaded collagen hydrogel as a dynamic bioadhesive dressing for diabetic wound repair.

J Mater Chem B 2021 Jul;9(29):5887-5897

School of Pharmaceutical Sciences, Jiangnan University, Wuxi, 214122, China.

As one of the serious complications of diabetes, diabetic ulcers induce several clinical problems. Although a variety of wound dressings are commonly employed, their role is too simple to integrate wound adaptation, therapeutics and effectiveness. To meet this comprehensive need, an injectable hydrogel (Col/APG) consisting of collagen and polyethylene glycol was prepared and loaded with umbilical cord stem cell factor (SCF) for the treatment of diabetic wounds. The physico-chemical properties of the Col/APG hydrogel, including rheology, self-shaping and self-healing, were demonstrated to adapt to the wound. After loading with the SCF, the adhesion strength of the resulting Col/APG + SCF hydrogel was enhanced to 17 kPa and it also showed favorable biocompatibility. A rapid cellular response, sufficient collagen deposition and marked neovascularization were observed in the whole cortex defect model of a diabetic rat after the Col/APG + SCF hydrogel was applied. Additionally, the skew toward M2 macrophages, credited with providing the anti-inflammatory function, also existed in both hydrogel groups. These findings suggested that the Col/APG hydrogel is a desirable scaffold and the Col/APG hydrogel loaded stem cell factor as a dressing is a promising treatment for diabetic tissue regeneration.
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http://dx.doi.org/10.1039/d1tb01163dDOI Listing
July 2021

Metal-Organic Framework-Derived Hollow and Hierarchical Porous Multivariate Metal-Oxide Microspheres for Efficient Phosphoproteomics Analysis.

ACS Appl Mater Interfaces 2021 Jul 14;13(29):34762-34772. Epub 2021 Jul 14.

National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, P. R. China.

Pre-enrichment of the biological samples is a crucial step in phosphoproteomics research. At present, metal-oxide affinity chromatography (MOAC) is one of the most recognized enrichment strategy. Therefore, the design and preparation of a MOAC-based affinity material with better enrichment properties will be of great significance for the phosphoproteomics study. In this work, we obtained a novel multivariate metal-oxide microsphere ([email protected]@TiO) with a hollow and hierarchical porous structure through pyrolysis of TiOmodified Fe/Ni-based metal-organic frameworks (MOFs). After pyrolysis, the carbon matrix derived from the MOFs provided support and porous properties. Meanwhile, multivariate metal oxides endowed the microspheres with an excellent magnetic response property and superior enrichment performance for phosphorylated biomolecules. The unique hollow and hierarchical porous structure greatly enhanced the diffusion of phosphorylated biomolecules. Therefore, the microspheres exhibited excellent enrichment performance for phosphorylated biomolecules: a large adsorption capacity (124 μmol g), excellent selectivity (α-casein/BSA, 1:5000, m/m), perfect size-exclusion performance (α-casein digests/α-casein/BSA, 1:500:500), and extremely low detection limit (2 fmol). Furthermore, the microspheres showed excellent enrichment performance in a series of real biological samples, such as nonfat milk, serum, saliva, rat brain tissue, and plasma exosomes of patients with esophageal cancer, which further demonstrated its huge application potential in MS-based phosphoproteomics research.
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http://dx.doi.org/10.1021/acsami.1c10795DOI Listing
July 2021

Characterization of isolates of members of the genus from : description of sp. nov., sp. nov., and sp. nov.

Int J Syst Evol Microbiol 2021 Jul;71(7)

State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.

Six novel strains (ZJ34, ZJ561, ZJ750, ZJ1629, zg-993 and zg-987) isolated from faeces and respiratory tracts of from the Qinghai-Tibet Plateau of PR China were characterized comprehensively. The results of analyses of the 16S rRNA gene and genome sequences indicated that the six strains represent three novel species of the genus , and are closely related to DSM 15434 (16S rRNA gene sequences similarities, 94.9-98.7 %), CCUG 61299 (95.6-96.6 %), CCTCC AB2010168 (95.7 %) and DSM 15435 (95.2-96.4 %), with values of digital DNA-DNA hybridization less than 30.1 % when compared with their closest relatives but higher than 70 % within each pair of novel strains (ZJ34/ZJ561, ZJ750/ZJ1629 and zg-993/zg-987). All the novel strains had C 9 and C as the two most abundant major fatty acids. MK-9(H) or MK-8(H) was the sole or predominant respiratory quinone of strains ZJ34, ZJ750 and zg-993 and their polar lipid profiles differed, but all had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and phosphatidyl inositol mannoside as major components. ZJ750 shared identical peptidoglycan amino acid profile with ZJ34 (alanine, glutamic acid, lysine and ornithine) and the same whole-cell sugar composition with zg-993 (glucose, rhamnose and ribose). Strain zg-993 contained alanine, aspartic acid, glutamic acid, glycine and lysine in the peptidoglycan, and the only sugar in ZJ34 was ribose. The DNA G+C contents of the novel strains were within the range of 65.8-70.1 mol%. On the basis of the results from the aforementioned analyses, the six novel strains were classified as representing three novel species of genus , for which the names sp. nov. [type strain ZJ34 (=GDMCC 1.1952=JCM 34355)] sp. nov. [type strain ZJ750 (=GDMCC 1.1950=JCM 34356)] and sp. nov. [type strain zg-993 (=GDMCC 1.1956=JCM 34357)] were proposed, respectively.
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http://dx.doi.org/10.1099/ijsem.0.004875DOI Listing
July 2021

Anti-inflammatory iron chelator, DIBI, reduces leukocyte-endothelial adhesion and clinical symptoms of LPS-induced interstitial cystitis in mice.

Clin Hemorheol Microcirc 2021 Jul 3. Epub 2021 Jul 3.

Department of Anesthesia, Pain Management and Perioperative Medicine, Dalhousie University, Halifax, NS, Canada.

Background: Interstitial cystitis (IC) is a prevalent and debilitating chronic inflammatory disease of the urinary bladder. Currently there are no fully effective therapeutic agents available, in part due to the still obscure pathogenesis of IC. Lipopolysaccharide (LPS) also known as endotoxin from Gram negative bacteria elicits IC in mice and has formed the basis of model systems for investigation. Excess free iron plays an important role in inflammation through generation of reactive oxygen species (ROS). The novel iron chelator DIBI has been shown to sequester excess free iron and dampen excess inflammatory responses to systemic LPS administration and also to Gram negative bacterial infections.

Objective: The overall objective of this study was to evaluate the effects of DIBI on LPS induced IC in mice. Leukocyte activation, endothelial adhesion and functional capillary density were assessed by intravital microscopy of the bladder microcirculation following a single intravesical LPS administration with or without intravesical DIBI treatment. Clinical IC symptoms were also assessed through behavioral and pain threshold force measurements.

Methods: Four groups of female BALB/c mice (n = 5-6/group) were randomized in this study: control group, IC group without therapy, IC group with DIBI therapy and control group with DIBI therapy. The groups were examined using intravital microscopy (IVM) of the bladder for leukocyte-endothelial interactions (adherent leukocytes, temporarily interacting leukocytes) and functional capillary density (FCD). A modified behavioral score by Boucher et al. and Von-Frey-Aesthesiometry were used to evaluate key behavioral indices related to pain and visceral pain perception.

Results: LPS introduced intravesically induced an early (≤2h) inflammation of the bladder evidenced by leukocyte activation and adhesion to bladder capillary walls. Intravesical DIBI therapy of mice 30min following LPS administration and assessed after 1.5h treatment showed a significant decrease in the number of adherent leukocytes compared to IC animals without DIBI treatment. DIBI treated mice showed a significantly lowered increase in behavioral distress scores compared to IC mice without therapy. Untreated IC mice exhibited a significantly decreased threshold force value for evoked pain response and DIBI treatment improved the threshold pain response. A significant inverse correlation was found for the two pain and suffering evaluation methods results.

Conclusion: DIBI reduced inflammatory endothelial leukocyte adhesion and key indices related to pain and suffering over those observed in untreated IC mice. Our findings suggest a potential therapeutic role for DIBI for IC treatment.
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http://dx.doi.org/10.3233/CH-201078DOI Listing
July 2021

Long Noncoding RNA CTD-2589M5.4 Inhibits Ovarian Cancer Cell Proliferation, Migration, and Invasion Via Downregulation of the Extracellular Matrix-Receptor Interaction Pathway.

Cancer Biother Radiopharm 2021 Jul 8. Epub 2021 Jul 8.

Department of Gynecology, Women's Hospital of Nanjing Medical University (Nanjing Maternity and Child Health Care Hospital), Nanjing, China.

The authors' previous study showed that the long noncoding RNA CTD-2589M5.4 was significantly upregulated in multidrug-resistant ovarian cancer cells. However, the role of CTD-2589M5.4 in the progression of ovarian cancer remains unclear. The purpose of this current study was to illuminate the biological function and possible mechanism of CTD-2589M5.4 in ovarian cancer development. The expression of CTD-2589M5.4 was examined via real-time quantitative PCR in primary ovarian cancer tissues (POCTs) and ovarian cancer cell lines. The biological function of CTD-2589M5.4 was analyzed via CCK-8 proliferation, wound healing, transwell, and flow cytometry assays in CTD-2589M5.4-overexpressed/silenced and control ovarian cancer cells. The mechanism of CTD-2589M5.4 function in ovarian cancer progression was analyzed utilizing high-throughput RNA-sequencing, Kyoto Encyclopedia of Genes and Genomes analysis, qRT-PCR, western blot, and rescue experiments. CTD-2589M5.4 expression was decreased in the POCTs and ovarian cancer cells compared with the normal ovarian tissues ( < 0.05) and normal ovarian epithelial cells ( < 0.05). Overexpression of CTD-2589M5.4 inhibited the proliferation, invasion, and migration of ovarian cancer cells, while knockdown of CTD-2589M5.4 had the opposite effect. Furthermore, a total of 750 and 233 genes were notably upregulated and downregulated, respectively, in the CTD-2589M5.4-overexpressed A2780 cells, while the extracellular matrix (ECM)-receptor interaction pathway was significantly downregulated. In addition, overexpression of fibronectin 1 significantly abrogated the tumor suppressive function of CTD-2589M5.4. This study demonstrated that CTD-2589M5.4 could inhibit ovarian cancer cell proliferation, invasion, and migration, at least partially by way of downregulating the ECM-receptor interaction pathway, therefore providing a potential therapeutic target for the prevention and/or treatment of ovarian cancer.
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http://dx.doi.org/10.1089/cbr.2020.4429DOI Listing
July 2021
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