Publications by authors named "Juan Zhang"

1,883 Publications

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Clinical characteristics and outcomes of chronic kidney disease in people living with HIV in a resource limited centre of central China.

AIDS Res Hum Retroviruses 2022 Aug 11. Epub 2022 Aug 11.

Wuhan University Zhongnan Hospital, Department of Infectious Diseases, Zhongnan Hospital of Wuhan University, 169 Donghu Road, Wuhan, China, 430071;

Background: Clinical management and optimal treatment are essential to improving outcomes for people living with HIV (PLWH). We assessed trends and outcomes of chronic kidney disease (CKD) in PLWH in a resource limited centre of central China.

Methods: All PLWH who were followed up in a tertiary referral centre in Wuhan, China, from July 2016 to June 2021 were evaluated. CKD was defined as glomerular filtration rate (GFR) < 60 ml/min/1.73m2 during two consecutive measurements three months apart. Baseline characteristics of the participants were extracted from the hospital medical records. The prevalence rate and associated risk factors of CKD were analyzed.

Results: A total of 863 PLWH with normal kidney function at baseline were analyzed. The median age was 33 (IQR: 26-49) years, 778 (90.2%) were male and 85 (9.8%) were female. Among them, 50 (5.8%) had their GFR falling below 60 ml/min/1.73m2 after a median of 54 months. Adjusted multivariate logistic regression revealed older age (aOR = 1.04, 95% CI: 1.01-1.07), female sex (aOR = 3.17, 95% CI: 1.14-8.84), lower body weight (aOR = 0.95, 95% CI: 0.91-1.00), lower hemoglobin (aOR = 3.54, 95% CI: 1.51-8.30), longer duration of antiretroviral therapy (ART) exposure (aOR = 1.02, 95% CI: 1.00-1.04), and a baseline GFR between 60-90 mL/min/1.73m2 (aOR = 3.89, 95% CI: 1.21-12.46) were associated with the development of CKD.

Conclusions: Our findings showed that CKD is not infrequent in PLWH with a combination of traditional and HIV-specific risk factors for kidney disease, highlighting the suboptimal monitoring and treatment options of CKD in PLWH in resource limited settings. Scalable monitoring strategy to improve care for this population is warranted.
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http://dx.doi.org/10.1089/AID.2022.0009DOI Listing
August 2022

Overlapping syndrome of recurrent anti-N-methyl-D-aspartate receptor encephalitis and anti-myelin oligodendrocyte glycoprotein demyelinating diseases: A case report.

World J Clin Cases 2022 Jun;10(18):6148-6155

Department of Neurology, Changzhi People's Hospital, Changzhi 046000, Shanxi Province, China.

Background: Anti-N-methyl-D-aspartate receptor encephalitis (NMDARe) is capable of presenting a relapsing course and coexisting with myelin oligodendrocyte glycoprotein antibody disease, whereas it has been relatively rare. We describe a man with no history of tumor who successively developed anti-NMDARe and anti-myelin oligodendrocyte glycoprotein antibody disease.

Case Summary: A 29-year-old man was initially admitted with headache, fever, intermittent abnormal behavior, decreased intelligence, limb twitching and loss of consciousness on July 16, 2018. On admission, examination reported no abnormality. During his presentation, he experienced aggravated symptoms, and the re-examination of cranial magnetic resonance imaging (MRI) indicated punctate abnormal signals in the left parietal lobe. External examination of cerebrospinal fluid and serum results revealed serum NMDAR antibody (Ab) (-), cerebrospinal fluid NMDAR-Ab (+) 1:10 and Epstein-Barr virus capsid antigen antibody IgG (+). Due to the imaging findings, anti-NMDARe was our primary consideration. The patient was treated with methylprednisolone and gamma globulin pulse therapy, mannitol injection dehydration to reduce intracranial pressure, sodium valproate sustained-release tablets for anti-epilepsy and olanzapine and risperidone to mitigate psychiatric symptoms. The patient was admitted to the hospital for the second time for "abnormal mental behavior and increased limb movements" on December 14, 2018. Re-examination of electroencephalography and cranial MRI showed no abnormality. The results of autoimmune encephalitis antibody revealed that serum NMDAR-Ab was weakly positive and cerebrospinal fluid NMDAR-Ab was positive. Considering comprehensive recurrent anti-NMDARe, the patient was treated with propylene-hormone pulse combined with immunosuppressive agents (mycophenolate mofetil), and the symptoms were relieved. The patient was admitted for "hoarseness and double vision" for the third time on August 23, 2019. Re-examination of cranial MRI showed abnormal signals in the medulla oblongata and right frontal lobe, and synoptophore examination indicated concomitant esotropia. The patient's visual acuity further decreased, and the re-examination of cranial MRI + enhancement reported multiple scattered speckled and patchy abnormal signals in the medulla oblongata, left pons arm, left cerebellum and right midbrain, thalamus. The patient was diagnosed with an accompanying demyelinating disease. Serum anti-myelin oligodendrocyte glycoprotein 1:10 and NMDAR antibody 1:10 were both positive. The patient was diagnosed with myelin oligodendrocyte glycoprotein antibody-related inflammatory demyelinating disease of the central nervous system complicated with anti-NMDARe overlap syndrome. The patient was successfully treated with methylprednisolone, gamma globulin pulse therapy and rituximab treatment. The patient remained asymptomatic and follow-up MRI scan 6 mo later showed complete removal of the lesion.

Conclusion: We emphasize the rarity of this antibody combination and suggest that these patients may require longer follow-up due to the risk of recurrence of two autoimmune disorders.
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http://dx.doi.org/10.12998/wjcc.v10.i18.6148DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254216PMC
June 2022

Restoration of Lower Facial and Neck Esthetics by Application of Pre-Expanded Bipedicle Scalp and Free Scapular Flaps.

J Craniofac Surg 2022 Aug 11. Epub 2022 Aug 11.

Department of Plastic and Reconstructive Surgery, Xijing Hospital of Forth Military Medical University, Xi'an City, Shaanxi Province, People's Republic of China.

Objective: To introduce the surgical procedures and experiences in restoration of lower facial esthetics for a male and a female patients who suffered from burn injuries. The difference of surgical methods which varied from sexes were discussed.

Materials And Methods: The clinical data of the patients was collected and retrospectively analyzed. The cervical contracture was repaired by pre-expanded free scapular flaps with sufficient pliable tissue for large defects. Moreover, for the male patient, prefabricated double pedicle scalp flap was applied to rebuild the beard region. The patients were followed up for 2 years. Surgical procedures and outcomes were detailed in the article.

Results: All the flaps were survived well. The facial hair-bearing area of the man was recovered with natural appearance. The reconstructed mental cervical angels and mandibular margins were clear. The neck extension mobility was improved. The 2 patients were satisfied with the outcomes of the treatments.

Conclusion: The treatments of facial and neck scar varied from sexes and different requirements. For male patient, the combination of scalp and scapular flaps was a feasible method to achieve natural beard and cervical appearance. For female patient, bilateral expanded scapular flaps could meet the demands of large tissue supply and a relative hidden donor site.
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http://dx.doi.org/10.1097/SCS.0000000000008918DOI Listing
August 2022

Challenges faced when living with schizophrenia in the community: A narrative inquiry.

Int J Soc Psychiatry 2022 Aug 9:207640221109168. Epub 2022 Aug 9.

School of Nursing, Wuhan University, China.

Background: Schizophrenia is a chronic and severe mental disorder. People with schizophrenia have transferred from hospital-based care to community-based care with the support of mental health legal policies. Challenges faced in the community should be emphasized. Limited qualitative studies have explored the challenges of living with schizophrenia.

Aims: To explore the challenges of people living with schizophrenia in the community.

Methods: A narrative method was used, including semi-structured, face-to-face interviews. Thematic analysis approach was used to analyze data.

Results: Ten clients and their family members participated in the study. Analysis revealed three main themes related to their challenges in the community: deficits in self-management of illness; insufficient community mental health care; and the influence of policy. These challenges prevent those with schizophrenia from effectively managing their illness, returning to a productive role in society, and improving their quality of life.

Conclusions: There are challenges in mental health rehabilitation and social reintegration of individuals with schizophrenia. There is a need for continuous community mental rehabilitation services, appropriate policy support, and the need to educate the public to reduce social bias and discrimination which allows individuals with schizophrenia to assume a productive role in the community.
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http://dx.doi.org/10.1177/00207640221109168DOI Listing
August 2022

Expression and Significance of TNF-α and NF-κB/p65 in Cutaneous Lichen Planus.

Clin Cosmet Investig Dermatol 2022 2;15:1509-1516. Epub 2022 Aug 2.

The Dermatology and Venereology Department, The Affiliated Hospital of Inner Mongolia Minzu University, Tongliao, People's Republic of China.

Purpose: The purpose of this study was to explore the expression of TNF-α and NF-κB/p65 in Lichen planus skin lesions and their correlation with the pathogenesis of Lichen planus.

Patients And Methods: The case group consisted of 30 individuals diagnosed with LP based on clinical and histopathologic examination. The control group consisted of 10 individuals from an Orthopedic Department with normal skin. TNF-α and NF-κB/p65 expression in skin tissue samples was detected by immunohistochemistry.

Results: The immunohistochemical results showed that TNF-α and NF-κB/p65 expression levels were significantly higher in LP skin lesions than normal skin tissues (P ≤ 0.05). Positive TNF-α staining mainly occurred in the cytoplasm of keratinocytes of the stratum granulosum, stratum spinosum, and stratum basale in the epidermis and lymphocytes in the superficial dermis. Positive NF-κB/p65 staining mainly occurred in the nucleus and cytoplasm of keratinocytes of the stratum spinosum and stratum basale in the epidermis and lymphocytes in the superficial dermis.

Conclusion: TNF-α and NF-κB/p65 are overexpressed in cutaneous LP. The two are positively correlated in LP, suggesting that they both play essential roles in the pathogenesis of LP.
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http://dx.doi.org/10.2147/CCID.S372662DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356624PMC
August 2022

Enhancing deep mineralization of refractory benzotriazole via carbon nanotubes-intercalated cobalt copper bimetallic oxide nanosheets activated peroxymonosulfate process: Mechanism, degradation pathway and toxicity.

J Colloid Interface Sci 2022 Jul 28;628(Pt A):448-462. Epub 2022 Jul 28.

State Key Laboratory of High-efficiency Coal Utilization and Green Chemical Engineering, College of Chemistry and Chemical Engineering, Ningxia University, Yinchuan 750021, China.

Peroxymonosulfate-based advanced oxidation processes (PMS-AOPs) are effective methods for the degradation of highly toxic and refractory nitrogen-containing heteroatomic pollutants such as benzotriazole (BTA). The construction of catalytic materials with multiple active centers is the key to generating abundant reactive oxygen species (ROS) and achieving high mineralization efficiency in PMS-AOPs. Herein, carbon nanotubes-intercalated cobalt copper bimetallic oxide nanosheets catalyst ([email protected]) was obtained by pyrolysis of two-dimensional (2D) MOF precursor. The degradation rate constant of BTA in [email protected]/PMS system was 4 times higher than that of metal oxide nanosheets catalyst without CNTs, while exhibiting high cycling stability and mineralization efficiency. Serial characterizations demonstrated that CoCu nanosheets was formed by CNTs-induced the directional assembly of metal oxide nanoparticles, which had high graphitization and abundant oxygen vacancies and could greatly facilitated the adsorption and electron transfer between the catalyst, PMS and BTA. Moreover, the doping of Cu species significantly improved PMS utilization and accelerated the Co(III)/Co(II) redox cycle. Both radicals (SO4• and •OH) and non-radicals (O) played a role in [email protected]/PMS system and the contributions of ROS were 72.2%, 11.1% and 16.7%, respectively. Meanwhile, the concentration of key ROS (SO) production increased from 4.76 μM to 8.56 μM compared with cobalt oxide nanosheets (CoNS). Three degradation pathways of BTA were proposed: benzene ring opening, benzene ring hydroxylation and triazole ring dimerization. Finally, the toxicity changes during the degradation process were measured and the toxicity of eleven intermediates was evaluated. This study may provide new insights into the degradation of persistent organic pollutants.
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http://dx.doi.org/10.1016/j.jcis.2022.07.162DOI Listing
July 2022

Ultrafast Laser-Ablated Bioinspired Hydrogel-Based Porous Gating System for Sustained Drug Release.

ACS Appl Mater Interfaces 2022 Aug 1;14(31):35366-35375. Epub 2022 Aug 1.

Department of Clinical Laboratory, The First Affiliated Hospital of USTC Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230027, China.

Gating systems have been extensively researched in energy harvesting, lab-on-chip applications, and so forth. However, the controlled drug delivery system with artificial hydrogel-based porous gating systems (HPGSs) is rarely reported. Herein, a biomimetic HPGS with a pH-responsive hydrogel as the valve and polydimethylsiloxane as the frame is fabricated by in situ femtosecond laser microdrilling and subsequent ultraviolet exposure. The proposed HPGS loaded with doxorubicin hydrochloride (DOX) is stable under physiological conditions, has a low drug leakage rate, and can achieve sustained drug release in a low pH environment. The experimental results show that the drug release is mainly controlled by non-Fickian diffusion, which renders the dynamic speed control of molecular transport possible. Moreover, the HPGS can also be prepared into an antitumor microcapsule. The results of in vitro cell experiments demonstrate that [email protected] can release drugs and achieve terrific therapeutic efficacy in the elimination of HeLa cells in the acidic environments around tumor cells. This functional HPGS is envisioned to be an ideal pH-response carrier for sustained drug release treatment of digestive diseases such as inflammatory bowel disease and gastrointestinal cancer.
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http://dx.doi.org/10.1021/acsami.2c07319DOI Listing
August 2022

Apolipoprotein A1 is associated with pulmonary vascular resistance and adverse clinical outcomes in patients with pulmonary hypertension secondary to heart failure.

Pulm Circ 2022 Jul 1;12(3):e12096. Epub 2022 Jul 1.

Division of Cardiology, Nanjing First Hospital Nanjing Medical University Nanjing China.

Pulmonary hypertension secondary to heart failure (HF-PH) combined with pulmonary vascular remodeling has a high mortality rate. Apolipoprotein A1 (ApoA1) has been shown to adversely affect outcomes in patients with HF. A prospective follow-up study was performed on 239 consecutive patients with HF-PH who underwent right heart catheterization. Proteomics technology was used to analyze different proteins in plasma between post- and precapillary pulmonary hypertension (CpcPH) and isolated postcapillary pulmonary hypertension (IpcPH) filtered by propensity score matching. Ultimately, 175 patients were enrolled and followed for an average of 4.4 years. Lipoprotein components in plasma were measured, and the following clinical events were tracked. Proteomics data showed that lipid metabolism and inflammation were different between CpcPH and IpcPH. ApoA1 levels in HF-PH patients with CpcPH were lower than those in HF-PH patients with IpcPH. The patients with lower ApoA1 levels (≤1.025 g/L) were in a higher New York Heart Association class and had high levels of NT-proBNP, mean pulmonary artery pressure, PVR, and diastolic pressure gradient. Besides, HF-PH patients with lower ApoA1 levels had a 2.836-fold higher relative risk of comorbid CpcPH compared with patients with higher ApoA1 levels. Moreover, patients with lower ApoA1 levels had a lower survival rate after adjusting for CpcPH. In conclusion, ApoA1 levels were negatively correlated with PVR levels. Lower ApoA1 levels were an independent risk factor for pulmonary vascular remodeling in HF-PH patients. The survival of HF-PH patients with lower ApoA1 levels was reduced.
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http://dx.doi.org/10.1002/pul2.12096DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9326519PMC
July 2022

-Thiohydroxy Succinimide Esters (NTSEs): Versatile Reagents for Selective Acyl and Acylthio Transfer.

Org Lett 2022 Aug 29;24(31):5736-5740. Epub 2022 Jul 29.

College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, People's Republic of China.

Differentiation between similarly reactive sites in molecules represents an ongoing challenge of organic synthesis. Herein we described one kind of versatile reagents, -thiohydroxy succinimide esters (NTSEs), serving as both acyl and acylthio surrogates for the diverse synthesis of ketones, thioesters, amides, and acyl disulfides by selective cleavage of similarly reactive C-S and N-S bonds.
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http://dx.doi.org/10.1021/acs.orglett.2c02160DOI Listing
August 2022

[The preference for Front-of-Pack Labeling and its association with the understanding of Nutrition Facts Panel among residents aged 18 to 70: results of a survey in 6 provinces of China].

Zhonghua Yu Fang Yi Xue Za Zhi 2022 Jul;56(7):952-959

School of Population Medicine and Public Health, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China.

To investigate the preference for Front-of-Pack Labeling (FOP) and its association with the understanding of the Nutrition Facts Panel among Chinese residents. A multi-stage sampling strategy was adopted to select 3 002 people aged between 18 and 70 years old from the eastern region of China (Beijing, Jiangsu Province, Guangdong Province), the northeast region (Heilongjiang Province), the central region (Henan Province) and the western region (Sichuan Province) from July 2020 to March 2021. Socio-demographic characteristics of participants and their understanding of the Nutrition Facts Panel and preference for FOP were collected. The ² test was conducted to compare the preference for FOP in different groups of population, and multivariate logistic regression was used to analyze the association between the preference for FOP and the understanding of the Nutrition Facts Panel. The mean age of 3 002 participants was (42.3±13.4) years, of which 1 914 (63.8%) were females and 69.3% could not understand the Nutrition Facts Panel. About 2 458 respondents (81.9%) suggested that FOP could be promoted. The top three nutrients that should be labeled were sugar (68.4%), salt (68.2%) and total fat (62.4%). The number of participants who believed that the Multiple Traffic Lights (MTL) could be easier to help consumers to quickly choose healthy food, attract attention and provide the most needed information was 1 064 (35.4%), 1 026 (34.2%) and 1 140 (38.0%), respectively. The multivariate logistic regression analysis showed that, compared with the Guideline Daily Amount (GDA) system, participants who could not understand the Nutrition Facts Panel preferred (1) Nutri-Score, Warning labels, and Health logos: Smart Choice in terms of"Which format of FOP could quickly help you choose food more easily?"[ (95%): 2.21 (1.62-3.02), 1.64 (1.22-2.22), 1.79 (1.31-2.45), respectively]; (2) Nutri-Score, Warning labels, and Health logos: Smart Choice in terms of"Which format of FOP could attract your attention the most?"[ (95%): 2.62 (1.92-3.59), 1.96 (1.45-2.66), 2.25 (1.66-3.04), respectively]; and (3) Nutri-Score, Warning labels, and Health logos: Smart Choice in terms of"Which format of FOP could provide you with the most needed information?"[ (95%): 2.33 (1.70-3.21), 2.21 (1.66-2.95), 2.01 (1.50-2.71), respectively]. The residents from six provinces in China have a supportive attitude towards FOP. The interpretive FOP with color information, specific nutrient information and summary indicator can be launched. The nutrition information of sugar, salt and total fat could be prioritized to be labeled on the FOP.
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http://dx.doi.org/10.3760/cma.j.cn112150-20211102-01013DOI Listing
July 2022

miRNA-mRNA associations with inosine monophosphate specific deposition in the muscle of Jingyuan chicken.

Br Poult Sci 2022 Jul 27. Epub 2022 Jul 27.

College of Agriculture, Ningxia University, Yinchuan China.

1. Inosine monophosphate (IMP), is an essential component for meat flavour and microRNAs (miRNAs) play a vital role in its post-transcriptional regulation. However, the mechanism of how miRNA expression affects muscle-specific IMP deposition is unclear.2. The following study performed transcriptome sequencing and bioinformatics analysis of breast and leg muscle, which have significantly different IMP content in Jingyuan chicken. The differential miRNA-mRNAs were screened out and correlation analysis with IMP content was performed.3. A total of 39 differentially expressed miRNAs (DE miRNAs) and 666 differentially expressed mRNAs (DE mRNAs) were identified between breast muscles and leg muscles. Using miRNA-mRNA integrated analysis, 29 miRNA-target gene pairs were obtained, composed of 13 DE miRNAs and 28 DE mRNAs. Next, purine metabolism, glycolysis/gluconeogenesis, pyruvate metabolism and the biosynthesis of amino acid pathways as necessary for muscle IMP-specific deposition were identified. The differentially expressed gene , which was significantly enriched in all four pathways, is involved in IMP anabolism in the form of energy metabolism and enzyme activity regulation. The correlation analysis suggested that the gga-miR-107-3p-KLHDC2 negative interaction may be a key regulator in IMP deposition.4. This study explores the functional mechanism of IMP-specific deposition in Jingyuan chicken muscles at the miRNA and mRNA levels and highlights multiple candidate miRNAs and mRNAs for molecular-assisted breeding.
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http://dx.doi.org/10.1080/00071668.2022.2106777DOI Listing
July 2022

Impact of Obesogenic Environments on Sugar-Sweetened Beverage Consumption among Preschoolers: Findings from a Cross-Sectional Survey in Beijing.

Nutrients 2022 Jul 12;14(14). Epub 2022 Jul 12.

School of Population Medicine and Public Health, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.

The excessive consumption of sugar-sweetened beverages (SSBs) has been proven to be critical for obesity among preschoolers. This study aimed to describe the SSB consumption rates among preschoolers in the Dongcheng District of Beijing, China, and to explore the association between obesogenic environmental determinants and consumption. We applied a stratified cluster sampling method and recruited 3057 primary caregivers of preschoolers in June 2019 to participate in the survey. The caregivers reported their children's consumption rates of six categories of SSBs and their exposure rates to SSB-related obesogenic environments. The associations between them were tested using multivariate logistic regression models. The mean (SD) age of the children was 5.6 (0.6) years and nearly half (48.3%) were girls. About 84.5% of the children had consumed SSBs over the past three months, and sugar-sweetened milk beverages had the highest consumption rate. Higher exposure to advertisements for the corresponding SSB categories in children, higher frequency rates of consuming SSBs and of taking children to fast-food restaurants in caregivers, and lower frequency rates of reading the Nutrition Facts Panels by caregivers were associated with higher SSB consumption rates among children ( < 0.05 in all of the SSB categories investigated, except for the Nutrition Facts Panel reading behaviors for the sports and energy beverages). SSB consumption among preschoolers is of concern, and comprehensive policy actions and education are urgently needed.
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http://dx.doi.org/10.3390/nu14142860DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9321344PMC
July 2022

Dynamic Prediction of Mechanical Thrombectomy Outcome for Acute Ischemic Stroke Patients Using Machine Learning.

Brain Sci 2022 Jul 18;12(7). Epub 2022 Jul 18.

Department of Pharmacy, Nanjing First Hospital, Nanjing Medical University, Nanjing 210029, China.

The unfavorable outcome of acute ischemic stroke (AIS) with large vessel occlusion (LVO) is related to clinical factors at multiple time points. However, predictive models used for dynamically predicting unfavorable outcomes using clinically relevant preoperative and postoperative time point variables have not been developed. Our goal was to develop a machine learning (ML) model for the dynamic prediction of unfavorable outcomes. We retrospectively reviewed patients with AIS who underwent a consecutive mechanical thrombectomy (MT) from three centers in China between January 2014 and December 2018. Based on the eXtreme gradient boosting (XGBoost) algorithm, we used clinical characteristics on admission ("Admission" Model) and additional variables regarding intraoperative management and the postoperative National Institute of Health stroke scale (NIHSS) score ("24-Hour" Model, "3-Day" Model and "Discharge" Model). The outcome was an unfavorable outcome at the three-month mark (modified Rankin scale, mRS 3-6: unfavorable). The area under the receiver operating characteristic curve and Brier scores were the main evaluating indexes. The unfavorable outcome at the three-month mark was observed in 156 (62.0%) of 238 patients. These four models had a high accuracy in the range of 75.0% to 87.5% and had a good discrimination with AUC in the range of 0.824 to 0.945 on the testing set. The Brier scores of the four models ranged from 0.122 to 0.083 and showed a good predictive ability on the testing set. This is the first dynamic, preoperative and postoperative predictive model constructed for AIS patients who underwent MT, which is more accurate than the previous prediction model. The preoperative model could be used to predict the clinical outcome before MT and support the decision to perform MT, and the postoperative models would further improve the predictive accuracy of the clinical outcome after MT and timely adjust therapeutic strategies.
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http://dx.doi.org/10.3390/brainsci12070938DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9313360PMC
July 2022

The extent and nature of television food and non-alcoholic beverage advertising to children during chinese New Year in Beijing, China.

BMC Public Health 2022 07 26;22(1):1417. Epub 2022 Jul 26.

School of Population Medicine and Public Health, Peking Union Medical College / Chinese Academy of Medical Science, 100730, Beijing, China.

Background: Exposure to food and non-alcoholic beverage advertisements (F&B ads) on television, which can affect children's nutrition knowledge, food consumption, diet quality, and purchasing preferences, is one aspect of the obesogenic environment. This aspect has been well-studied and assessed in many countries. In China, however, only few studies have been done in earlier years and all of them were focus on regular days. This study aimed to assess the extent and nature of F&B ads on television (TV) during the public holiday directed towards children aged 4-14 years in Beijing.

Method: Top 3 channels viewed by children aged 4-14 years in Beijing were selected by TV viewership data, survey, and expert consultation. Each channel was recorded for 7 days (24 h) during the public holiday of the Chinese New Year in 2019. F&B ads were coded and analyzed following the adapted food promotion module of INFORMAS protocol. Three nutrient profile models were used to classify F&B ads as healthy or unhealthy F&B ads.

Results: Of the 10,082 ads in 504-hour recorded programs, 42.9% were F&B ads. The hourly average ads and F&B ads per channel were 19.8 (SD 15.32) and 8.6 (SD 9.84), while that was higher on the national children's channel (17.15, SD 12.25) than other channels (p < 0.05). Of F&B ads classified with the three nutrient profile models, more than 55% were unhealthy for children. The categories most frequently advertised were savory snacks, milk drinks, nonpermitted milk drinks, cakes/sweet biscuits, and beverages. Unhealthy F&B ads were more likely to use promotional characters, brand benefit claims, and health claims than permitted F&B ads (p < 0.05).

Conclusions: Children in Beijing were exposed to a high proportion of unhealthy F&B ads during the Chinese New Year holiday. Our findings support the need to assess and regulate TV F&B ads marketing for children.
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http://dx.doi.org/10.1186/s12889-022-13801-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9316732PMC
July 2022

Tumorigenic cell selection and substrate rigidity manipulation using 2D and 3D extracellular matrices.

STAR Protoc 2022 09 19;3(3):101577. Epub 2022 Jul 19.

Department of Oncology, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.

Soft fibrin gels are used to select tumorigenic cells and regulate the stemness and metastasis of colorectal cancer cells via mechanotransduction. This protocol details steps to produce two-dimensional (2D) and three-dimensional (3D) extracellular matrices for substrate rigidity manipulation and tumorigenic cell selection. We also describe how it can be applied to tumor mechanotransductive research by colony growth monitoring and cell isolation. For complete details on the use and execution of this protocol, please refer to Chang et al. (2022).
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http://dx.doi.org/10.1016/j.xpro.2022.101577DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9307683PMC
September 2022

Detection of the Content of Two Coumarins, IM and ISOIM, and Their Mechanism of Action on Colitis Rats in Angelica albicans.

Comput Math Methods Med 2022 16;2022:5475559. Epub 2022 Jul 16.

Department of Pharmacy, Third Hospital of Hebei Medical University, Shijiazhuang, 050000 Hebei, China.

is being used in the cure of different, respiratory, neuromuscular, and cutaneous diseases in traditional eastern medicine. The pharmacokinetic (PK) characteristics of imperatorin (IM) and isoimperatorin (ISOIM), the main effective components in , were investigated. The rapid, subtle, and measuring the PKs of a drug, a validated UPLC/MS/MS methodology was designed for a total of 2 furanocoumarins in 2,4,6-trinitrobenzene sulfonic acid-stimulated and untreated mice. After that, blood samples were obtained. (0.5 and 1.0 g/kg) was given orally, taken regularly from the tail vein. The time it takes for colitis rats to achieve their maximal concentration ( ) imperatorin and isoimperatorin was considerably postponed. In comparison to normal rats, all furanocoumarins had lesser peak plasma concentrations ( ) and higher represent residence durations. The area below the time-curve or clearance half-life did not differ significantly. In normal rats, all two furanocoumarins attained maximal plasma levels between 40 and 75 minutes, demonstrating fast oral absorption. The periods to attain of the two furanocoumarins, on the other hand, were shorter than in earlier studies. Therefore, colitis-linked alterations in the drug-absorption stage may result in a late and lowered , which have no effect on its clearance in half-life. Hence, conclusively, as a result, more consideration should be given to the prescription and administration of in colitis individuals, and more research is needed to determine whether the changed PK profile was clinically meaningful for medicinal dose.
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http://dx.doi.org/10.1155/2022/5475559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9308527PMC
July 2022

Acylhydrazone-based supramolecular assemblies undergoing a converse sol-to-gel transition on → photoisomerization.

Chem Sci 2022 Jul 16;13(26):7892-7899. Epub 2022 Jun 16.

Shaanxi Key Laboratory of Macromolecular Science and Technology, MOE Key Laboratory of Material Physics and Chemistry under Extraordinary Conditions, School of Chemistry and Chemical Engineering, Northwestern Polytechnical University Xi'an 710072 P. R. China

Photoisomeric supramolecular assemblies have drawn enormous attention in recent years. Although it is a general rule that photoisomerization from a less to a more distorted isomer causes the destruction of assemblies, this photoisomerization process inducing a converse transition from irregular aggregates to regular assemblies is still a great challenge. Here, we report a converse sol-to-gel transition derived from the planar to nonplanar photoisomer conversion, which is in sharp contrast to the conventional light-induced gel collapse. A well-designed acylhydrazone-linked monomer is exploited as a photoisomer to realize the above-mentioned phase transition. In the monomer, imine is responsible for - interconversion and amide generates intermolecular hydrogen bonds enabling the photoisomerization-driven self-assembly. The counterintuitive feature of the sol-to-gel transition is ascribed to the partial → photoisomerization of acylhydrazone causing changes in stacking mode of monomers. Furthermore, the reversible phase transition is applied in the valves formed in microfluidic devices, providing fascinating potential for miniature materials.
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http://dx.doi.org/10.1039/d2sc01657eDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258502PMC
July 2022

Computed tomography-guided radiofrequency ablation of cervical intervertebral discs for the treatment of refractory cervicogenic headache: A retrospective chart review.

Headache 2022 Jul 21;62(7):839-847. Epub 2022 Jul 21.

Department of Pain, Zhejiang Provincial People's Hospital, Hangzhou Medical College, Hangzhou, China.

Objective: To evaluate the feasibility and efficacy of computed tomography (CT)-guided radiofrequency ablation (RFA) of cervical intervertebral discs for the treatment of discogenic cervicogenic headache (CEH).

Background: Some patients with CEH experience no obvious therapeutic effect after conventional therapy, particularly patients with refractory CEH originating from abnormal cervical intervertebral discs. Treatment for this type of CEH remains poorly characterized.

Methods: Using a single intervention arm, pretest/posttest design, we retrospectively analyzed the data of patients who underwent CT-guided RFA of cervical intervertebral discs for CEH at the Pain Medicine Center of Zhejiang Provincial People's Hospital from January 2017 to April 2021. If conservative treatment failed in patients with discogenic CEH, we classified the patients as having refractory CEH and performed RFA of cervical intervertebral discs. We used a numeric rating scale (NRS) to assess pain intensity for 6 months. We also compared therapeutic outcome of patients with different characteristics.

Results: A total of 44 patients who underwent CT-guided RFA of cervical intervertebral discs were enrolled and 41 of them were analyzed in the present study. The preoperative median (25th, 75th) NRS score was 4 (4, 5), and it was significantly reduced to 1 (0, 4) 6 months after RFA (p < 0.001). The number of patients with ≥50% of their pain relieved after 6 months was 28 of 41 (68%). No serious treatment-related complications occurred in this study. Compared with single-level RFA, multi-level RFA shows greater effects on pain intensity reduction (p = 0.032) and pain relief rate (p = 0.047) of patients.

Conclusion: In patients who have discogenic CEH, CT-guided RFA of the cervical intervertebral discs appears to be a promising treatment with no serious complications.
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http://dx.doi.org/10.1111/head.14361DOI Listing
July 2022

Ovarian cancer risk of Chinese women with BRCA1/2 germline pathogenic variants.

J Hum Genet 2022 Jul 21. Epub 2022 Jul 21.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Familial and Hereditary Cancer Center, Peking University Cancer Hospital and Institute, 52 Fucheng Road, Haidian District, 100142, Beijing, China.

Estimating the lifetime risk of ovarian cancer in Chinese women with BRCA1/2 germline pathogenic variants (PVs) is of great importance for the clinical management of BRCA1/2 carriers. This cohort study recruited 9903 unselected Chinese breast cancer patients whose BRCA1/2 status was determined. Of these, 3984 probands completed family history questionnaires, which investigated the health status of their relatives, including 11,997 female first-degree relatives. The ovarian cancer risk of BRCA1/2 germline pathogenic carriers was estimated using the ovarian cancer history of proband first-degree female relatives via the Kin-cohort method. Of the 3984 probands, 126 (3.2%) carried BRCA1 PVs, and 183 (4.6%) carried BRCA2 PVs. The estimated cumulative risks of ovarian cancer by age 70 were 15.3% (95% CI 8.4-18.6%) for BRCA1 carriers, 5.5% (95% CI 2.0-10.2%) for BRCA2 carriers, and 0.4% (95% CI 0.3-0.7%) for noncarriers. The cumulative risks of ovarian cancer were very low before the age of 40 for both BRCA1 and BRCA2 carriers and were an increase up to age 45. The cumulative ovarian cancer risk of BRCA1 carriers was approximately three times higher than that of BRCA2 carriers, and BRCA1 and BRCA2 carriers had 38- and 14-fold higher risks than non-BRCA carriers, respectively. The findings indicate that Chinese women with BRCA1/2 PVs have high risks of ovarian cancer in their lifetime, especially BRCA1 carriers. These results are useful for devising optimal strategies to reduce ovarian cancer risk in BRCA1/2 carriers.
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http://dx.doi.org/10.1038/s10038-022-01065-6DOI Listing
July 2022

Left frontal eye field encodes sound locations during passive listening.

Cereb Cortex 2022 Jul 21. Epub 2022 Jul 21.

Department of Otorhinolaryngology, Head and Neck Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China.

Previous studies reported that auditory cortices (AC) were mostly activated by sounds coming from the contralateral hemifield. As a result, sound locations could be encoded by integrating opposite activations from both sides of AC ("opponent hemifield coding"). However, human auditory "where" pathway also includes a series of parietal and prefrontal regions. It was unknown how sound locations were represented in those high-level regions during passive listening. Here, we investigated the neural representation of sound locations in high-level regions by voxel-level tuning analysis, regions-of-interest-level (ROI-level) laterality analysis, and ROI-level multivariate pattern analysis. Functional magnetic resonance imaging data were collected while participants listened passively to sounds from various horizontal locations. We found that opponent hemifield coding of sound locations not only existed in AC, but also spanned over intraparietal sulcus, superior parietal lobule, and frontal eye field (FEF). Furthermore, multivariate pattern representation of sound locations in both hemifields could be observed in left AC, right AC, and left FEF. Overall, our results demonstrate that left FEF, a high-level region along the auditory "where" pathway, encodes sound locations during passive listening in two ways: a univariate opponent hemifield activation representation and a multivariate full-field activation pattern representation.
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http://dx.doi.org/10.1093/cercor/bhac261DOI Listing
July 2022

Trends of Blood Lead Levels in US Pregnant Women: The National Health and Nutrition Examination Survey (2001-2018).

Front Public Health 2022 1;10:922563. Epub 2022 Jul 1.

Jinan Maternity and Child Care Hospital Affiliated to Shandong First Medical University, Jinan, China.

Objectives: This study investigates the trends of blood lead levels in US pregnant women based on the National Health and Nutrition Examination Survey from 2001 to 2018.

Methods: A total of 1,230 pregnant women were included in this study. The weighted logistic regression was applied to analyze the association between sociodemographic characteristics with high blood levels. We computed the blood lead levels for each survey period from 2001-2002 to 2017-2018. Moreover, we used the adjusted linear regression model to investigate the time-related change in blood lead level. The odds ratio (OR) with a 95% confidence interval (CI) was calculated accordingly.

Results: The mean blood lead was 0.73 ± 0.03 ug/dL, and high blood lead was observed in 2.53% of individuals. The Mexican Americans were more associated with high blood lead than the non-Hispanic white (OR, 1.072; 95% CI, 1.032-1.112). The mean blood lead level has decreased from 0.97 ug/dL in 2001-2002 to 0.46 ug/dL in 2013-2014. Afterward, a slight increase was observed with the mean blood lead of 0.55 ug/dL in 2015-2016 and 0.53 ug/dL in 2017-2018. In the adjusted linear regression model, each year's increase would lead to a 0.029 ug/dL decrease in blood lead ( < 0.001). However, no significant change was observed in the 2017-2018 cycle compared with 2009-2010 ( = 0.218).

Conclusion: This study summarized the trend of blood lead levels in US pregnant women over 2001-2018. Continued effort is still required to control lead sources better and protect this population from lead exposure.
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http://dx.doi.org/10.3389/fpubh.2022.922563DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9283785PMC
July 2022

Social isolation reinforces aging-related behavioral inflexibility by promoting neuronal necroptosis in basolateral amygdala.

Mol Psychiatry 2022 Jul 15. Epub 2022 Jul 15.

Department of Pathophysiology, Key Lab of Neurological Disorder of Education Ministry, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Aging is characterized with a progressive decline in many cognitive functions, including behavioral flexibility, an important ability to respond appropriately to changing environmental contingencies. However, the underlying mechanisms of impaired behavioral flexibility in aging are not clear. In this study, we reported that necroptosis-induced reduction of neuronal activity in the basolateral amygdala (BLA) plays an important role in behavioral inflexibility in 5-month-old mice of the senescence-accelerated mice prone-8 (SAMP8) line, a well-established model with age-related phenotypes. Application of Nec-1s, a specific inhibitor of necroptosis, reversed the impairment of behavioral flexibility in SAMP8 mice. We further observed that the loss of glycogen synthase kinase 3α (GSK-3α) was strongly correlated with necroptosis in the BLA of aged mice and the amygdala of aged cynomolgus monkeys (Macaca fascicularis). Moreover, genetic deletion or knockdown of GSK-3α led to the activation of necroptosis and impaired behavioral flexibility in wild-type mice, while the restoration of GSK-3α expression in the BLA arrested necroptosis and behavioral inflexibility in aged mice. We further observed that GSK-3α loss resulted in the activation of mTORC1 signaling to promote RIPK3-dependent necroptosis. Importantly, we discovered that social isolation, a prevalent phenomenon in aged people, facilitated necroptosis and behavioral inflexibility in 4-month-old SAMP8 mice. Overall, our study not only revealed the molecular mechanisms of the dysfunction of behavioral flexibility in aged people but also identified a critical lifestyle risk factor and a possible intervention strategy.
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http://dx.doi.org/10.1038/s41380-022-01694-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284973PMC
July 2022

The Prominent Role of the Temporal Lobe in Premenstrual Syndrome and Premenstrual Dysphoric Disorder: Evidence From Multimodal Neuroimaging.

Front Psychiatry 2022 28;13:954211. Epub 2022 Jun 28.

Wuhan Mental Health Center, Wuhan, China.

Premenstrual syndrome (PMS) is a group of psychological, physical, and behavioral symptoms that recur with the menstrual cycle, usually occurring a few days before menstruation and ceasing with the onset of menstruation. Premenstrual dysphoric disorder (PMDD) is a severe form of PMS that has been included in a subcategory of depression in the Diagnostic and Statistical Manual of Mental Disorders (DSM-V) according to the latest diagnostic criteria. Patients usually present with mild to moderate emotional and physical symptoms that affect their routine work, social activities, and family lives. The pathogenesis of PMDD remains unclear, and some researchers believe that it is related to fluctuations in ovarian hormone levels. However, the details of the interrelationships and regulating effects between ovarian hormones, symptoms, and the brain need to be more comprehensively determined. Recent studies have revealed some novel findings on PMS and PMDD based on brain morphology, function, and metabolism. Additionally, multiple studies have suggested that PMS and PMDD are closely related to brain structural and functional variations in certain core temporal lobe regions, such as the amygdala and hippocampus. We summarized neuroimaging studies of PMS and PMDD related to the temporal lobe by retrospectively reviewing relevant literature over the past decade. This review contributes to further clarifying the significant role of the temporal lobe in PMS and PMDD and understanding the neurochemical links between hormones, symptoms, and the brain.
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http://dx.doi.org/10.3389/fpsyt.2022.954211DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9274249PMC
June 2022

Fluorescence imaging of FEN1 activity in living cells based on controlled-release of fluorescence probe from mesoporous silica nanoparticles.

Biosens Bioelectron 2022 Oct 2;214:114529. Epub 2022 Jul 2.

Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, China. Electronic address:

Flap endonuclease 1 (FEN1) is a structure-specific nuclease, which catalyzes the removal of 5' overhanging DNA flap from a specific DNA structure. FEN1 has been considered as an important biomarker for cancer diagnosis since it is over-expressed in various types of human tumor cells and closely related to cancer development. Nanoprobes gradually become basic tools for analyzing biomarkers variations in vivo. Here, we utilized aminoated mesoporous silica nanoparticles (NH-MSNs) with a rich porous structure as the fluorescence nanoprobes to entrap the rhodamine 6G (Rh6G) molecules. Then gold nanoparticles linked specific single-stranded DNA (AuNPs-ssDNA) as a molecular gate was used to coat the NH-MSNs surface. The fluorescence signal was weak when the fluorescence molecules were blocked by the AuNPs-ssDNA. In the presence of FEN1, it recognized and cleaved the specific ssDNA to release the Rh6G from NH-MSNs, which resulted in recovered fluorescence signals. Thus, the sensitive detection of FEN1 activity was realized by controlled-release of Rh6G. The fluorescence signal showed a good linear relationship with the logarithm of FEN1 activity ranging from 0.05 to 1.75 U with a detection limit of 0.03 U. Moreover, confocal imaging demonstrated that the proposed biosensor could distinguish tumor cells from normal cells. Therefore, this technique contributes to clinical diagnostic and therapeutic monitoring.
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http://dx.doi.org/10.1016/j.bios.2022.114529DOI Listing
October 2022

Engineering flexible loops to enhance thermal stability of keratinase for efficient keratin degradation.

Sci Total Environ 2022 Jul 9;845:157161. Epub 2022 Jul 9.

Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. Electronic address:

Keratinase-catalyzed degradation of keratin waste has been shown to be a promising recycling method. Although the recombinant KerZ1 derived from Bacillus subtilis has shown the highest activity among the keratinases reported so far, the low thermal stability caused by the unstable flexible loops limited its keratin-degrading ability. To this end, the flexible loops of KerZ1 were engineered to be more hydrophobic and rigid through B-factor calculations, molecular dynamics simulations, and β-turn redesign. We developed several highly thermostable keratinase variants and showed enhanced keratin degradation activity. In particular, the loop regions of the variants KerZ1, KerZ1 and KerZ1 were designed to be more stable, with T values increased by 8 °C, 6 °C and 5 °C, and corresponding t increased by 2.3, 3.3 and 5.0 times. The keratin degradation activity of the variant KerZ1 at 60 °C was enhanced by 46 % compared with KerZ1. The strategy of this research and the obtained keratinase variants will be a significant improvement in the complete degradation of keratin.
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http://dx.doi.org/10.1016/j.scitotenv.2022.157161DOI Listing
July 2022

Boronic acid grafted metal-organic framework for selective enrichment of cis-diol-containing compounds.

J Chromatogr A 2022 Aug 25;1677:463281. Epub 2022 Jun 25.

School of Chemistry and Environmental Engineering, Key Laboratory of Novel Biomass-Based Environmental and Energy Materials in Petroleum and Chemical Industry, Wuhan Institute of Technology, Wuhan 430205, China. Electronic address:

This study constructed boronic acid grafted Zr-MOF (BA-Zr-MOF) by a simple pre-installation strategy through mixed organic ligands. Typically, BA-Zr-MOF was prepared by one-step hydrothermal method used for enrichment of cis-diol-containing nucleosides through pipette tip solid-phase extraction (PT-SPE) followed by detection of high-performance liquid chromatography. It is worth mentioning that BA is well assembled into MOF and cis-diol-containing compounds can be efficiently and selectively enriched by abundant boronic acid groups. Three groups of different types of compounds were used to evaluate their selectivity and the results showed the excellent selectivity to cis-diol-containing compounds of as-prepared adsorbent. The BA-Zr-MOF adsorbent possesses a high adsorption capacity, which can reach 86.40 mg g for adenosine. Under the optimal extraction condition, a PT-SPE-HPLC method based on BA-Zr-MOF for analysis of nucleosides was established. The linear range of the four nucleosides is 0.01 to 50 µg mL with R ≥ 0.99 and the detection limits (LODs) are estimated at between 0.005-0.012 µg mL. The recoveries in urine were used to test the reliability of the analytical methods, which ranged from 82.8% to 117.1%, with intra-day relative standard deviations (RSDs) ranged from 0.1% to 4.2% and the inter-day RSDs ranged from 0.2% to 6.2%. All the results show that the pre-installation strategy based on dual ligands is an alternative to fabricate MOF composite material and BA-Zr-MOF is a promising material for the analysis of cis-diol-containing biomolecules.
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http://dx.doi.org/10.1016/j.chroma.2022.463281DOI Listing
August 2022

Effect of Qingre Jiedu Huoxue Huayu Recipe on blood stasis and toxin syndrome in patients with non-ST segment elevation acute coronary syndrome, serum Lp-PLA2, TNF- α, and PIGF expression level.

Cell Mol Biol (Noisy-le-grand) 2022 Jan 2;67(4):121-129. Epub 2022 Jan 2.

Department of Cardiovascular Diseases,The National Hospital of Enshi Hubei 445000 China.

The pathological basis of non-ST-segment elevation acute coronary syndrome (NSTE-ACS) is severe coronary stenosis, unstable plaque erosion, and rupture, resulting in coronary blood flow reduction and myocardial ischemia, leading to acute thrombosis cardiovascular disease events. This subject intends to study the treatment of NSTE-ACS patients with blood stasis and toxin syndrome by Qingre Jiedu Huoxue Huayu Decoction, observe its clinical efficacy, and explore the effects of serum lipoprotein phospholipase A2 (Lp-PLA2) and tumor necrosis factor- α (TNF- α), the effect of placental growth factor (PIGF) expression. In this study, 100 patients with blood stasis and toxin syndrome of NSTE-ACS treated in the cardiovascular department of Enshi National Hospital from August 2020 to August 2021 were selected as the research object. They were randomly divided into traditional Chinese medicine comprehensive treatment groups and conventional western medicine control groups, with 50 cases. The conventional western medicine control group was treated with hydroclopidogrel tablets orally, and the comprehensive treatment group of traditional Chinese medicine combined with Qingre Jiedu Huoxue Huayu formula orally. The patients in both groups were treated for four weeks. The results showed that after treatment, the practical clinical rate of the comprehensive treatment group was significantly higher than that of the conventional western medicine control group. After treatment, the TCM syndrome score, angina pectoris attack duration, and angina pectoris attack frequency, myocardial zymogram index level, serum Lp-PLA2 and TNF of the two groups were measured- α. The levels of PIGF were significantly lower than those before treatment. The decline of the above indexes in the comprehensive treatment group of traditional Chinese medicine was significantly better than that in the control group of conventional Western Medicine (P<0.05). The incidence of MACE events in the TCM Comprehensive treatment group was significantly lower than that in the conventional western medicine control group (P <0.05).
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http://dx.doi.org/10.14715/cmb/2021.67.4.14DOI Listing
January 2022

Role of Natural Compounds and Target Enzymes in the Treatment of Alzheimer's Disease.

Molecules 2022 Jun 29;27(13). Epub 2022 Jun 29.

Department of Neurology, Sanya Central Hospital, Hainan Third People's Hospital, Sanya 572000, China.

Alzheimer's disease (AD) is a progressive neurological condition. The rising prevalence of AD necessitates the rapid development of efficient therapy options. Despite substantial study, only a few medications are capable of delaying the disease. Several substances with pharmacological activity, derived from plants, have been shown to have positive benefits for the treatment of AD by targeting various enzymes, such as acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), β-secretase, γ-secretase, and monoamine oxidases (MAOs), which are discussed as potential targets. Medicinal plants have already contributed a number of lead molecules to medicine development, with many of them currently undergoing clinical trials. A variety of medicinal plants have been shown to diminish the degenerative symptoms associated with AD, either in their raw form or as isolated compounds. The aim of this review was to provide a brief summary of AD and its current therapies, followed by a discussion of the natural compounds examined as therapeutic agents and the processes underlying the positive effects, particularly the management of AD.
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http://dx.doi.org/10.3390/molecules27134175DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9268689PMC
June 2022

Corrigendum: Clinical Characteristics and Risk Factors for Severe Dengue Fever in Xishuangbanna, During the Dengue Outbreak in 2019.

Front Microbiol 2022 20;13:939709. Epub 2022 Jun 20.

Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming, China.

[This corrects the article DOI: 10.3389/fmicb.2022.739970.].
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http://dx.doi.org/10.3389/fmicb.2022.939709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253990PMC
June 2022

FOCAD/miR-491-5p, downregulated by EGR1, function as tumor suppressor by inhibiting the proliferation and migration of gastric cancer cells.

Prog Biophys Mol Biol 2022 Jul 3. Epub 2022 Jul 3.

Department of Pathology, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University 76 Yanta West Road, Xi'an, Shaanxi, PR China. Electronic address:

Gastric cancer is a common malignant tumor in China; however, its carcinogenesis remains unknown. Focadhesin (FOCAD) is a tumor suppressor gene in gliomas, its expression, role, and mechanism in gastric cancer have not been defined. The aim of the present study was to explore the expression pattern of FOCAD in human normal tissues and cancer tissues and elucidate the role and regulatory mechanism of Early Growth Response 1 (EGR1) in FOCAD and its intron, miR-491-5p, in gastric cancer. Immuno histochemical staining revealed that FOCAD is widely and highly expressed in normal gastric mucosa, but is absent in gastric cancer tissue. Based on an association analysis FOCAD expression was found to be negatively associated with lymph node metastasis (P = 0.004); higher FOCAD levels were associated with longer survival in patients with gastric cancer (P = 0.001). MTT, colony, Transwell chamber, and flow cytometry assays revealed that siFOCAD promoted cell proliferation, growth, and migration, and inhibited apoptosis. Furthermore, bioinformatic analysis, Fluorescence reporter gene and chromatin immunoprecipitation analyses confirmed that EGR1 binds to the promoter and negatively regulates FOCAD and miR-491-5p at the transcriptional level. The overexpression of EGR1 was also found to promote cell proliferation, growth, and migration, and inhibit apoptosis. Overall, FOCAD is specifically overexpressed in the gastric mucosa and is significantly downregulated in gastric cancer. To our knowledge, this is the first study to demonstrate that FOCAD is a tumor suppressor, higher FOCAD levels might be a better prognostic marker of gastric cancer, and FOCAD/miR-491-5p may be negatively regulated by EGR1.
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http://dx.doi.org/10.1016/j.pbiomolbio.2022.06.003DOI Listing
July 2022
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