Publications by authors named "Juan Zhang"

1,557 Publications

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Decreased Resting-State Functional Connectivity of Periaqueductal Gray in Temporal Lobe Epilepsy Comorbid With Migraine.

Front Neurol 2021 26;12:636202. Epub 2021 May 26.

Department of Neurology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Patients with temporal lobe epilepsy (TLE) are at high risk for having a comorbid condition of migraine, and these two common diseases are proposed to have some shared pathophysiological mechanisms. Our recent study indicated the dysfunction of periaqueductal gray (PAG), a key pain-modulating structure, contributes to the development of pain hypersensitivity and epileptogenesis in epilepsy. This study is to investigate the functional connectivity of PAG network in epilepsy comorbid with migraine. Thirty-two patients with TLE, including 16 epilepsy patients without migraine (EwoM) and 16 epilepsy patients with comorbid migraine (EwM), and 14 matched healthy controls (HCs) were recruited and underwent resting functional magnetic resonance imaging (fMRI) scans to measure the resting-state functional connectivity (RsFC) of PAG network. The frequency and severity of migraine attacks were assessed using the Migraine Disability Assessment Questionnaire (MIDAS) and Visual Analog Scale/Score (VAS). In animal experiments, FluoroGold (FG), a retrograde tracing agent, was injected into PPN and its fluorescence detected in vlPAG to trace the neuronal projection from vlPAG to PPN. FG traced neuron number was used to evaluate the neural transmission activity of vlPAG-PPN pathway. The data were processed and analyzed using DPARSF and SPSS17.0 software. Based on the RsFC finding, the excitatory transmission of PAG and the associated brain structure was studied via retrograde tracing in combination with immunohistochemical labeling of excitatory neurons. Compared to HCs group, the RsFC between PAG and the left pedunculopontine nucleus (PPN), between PAG and the corpus callosum (CC), was decreased both in EwoM and EwM group, while the RsFC between PAG and the right PPN was increased only in EwoM group but not in EwM group. Compared to EwoM group, the RsFC between PAG and the right PPN was decreased in EwM group. Furthermore, the RsFC between PAG and PPN was negatively correlated with the frequency and severity of migraine attacks. In animal study, a seizure stimulation induced excitatory transmission from PAG to PPN was decreased in rats with chronic epilepsy as compared to that in normal control rats. The comorbidity of epilepsy and migraine is associated with the decreased RsFC between PAG and PPN.
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http://dx.doi.org/10.3389/fneur.2021.636202DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8189422PMC
May 2021

DHODH inhibition modulates glucose metabolism and circulating GDF15, and improves metabolic balance.

iScience 2021 May 1;24(5):102494. Epub 2021 May 1.

Department of Microbiology, Tumor and Cell Biology (MTC), Biomedicum, Karolinska Institutet, SE-171 65 Stockholm, Sweden.

Dihydroorotate dehydrogenase (DHODH) is essential for the synthesis of pyrimidine ribonucleotides, and as such, its inhibitors have been long used to treat autoimmune diseases and are in clinical trials for cancer and viral infections. Interestingly, DHODH is located in the inner mitochondrial membrane and contributes to provide ubiquinol to the respiratory chain. Thus, DHODH provides the link between nucleotide metabolism and mitochondrial function. Here we show that pharmacological inhibition of DHODH reduces mitochondrial respiration, promotes glycolysis, and enhances GLUT4 translocation to the cytoplasmic membrane and that by activating tumor suppressor p53, increases the expression of GDF15, a cytokine that reduces appetite and prolongs lifespan. In addition, similar to the antidiabetic drug metformin, we observed that in mice, DHODH inhibitors elevate levels of circulating GDF15 and reduce food intake. Further analysis using this model for obesity-induced diabetes revealed that DHODH inhibitors delay pancreatic β cell death and improve metabolic balance.
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http://dx.doi.org/10.1016/j.isci.2021.102494DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8169992PMC
May 2021

Echocardiographic Parameters Predict Short- and Long-Term Adverse Cardiovascular Events in Patients with Acute Myocardial Infarction.

Int J Gen Med 2021 3;14:2297-2303. Epub 2021 Jun 3.

Heart Center and Beijing Key Laboratory of Hypertension Research, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, People's Republic of China.

Objective: This study aimed to find echocardiographic parameters that can predict short- and long-term adverse cardiovascular events in patients with AMI.

Methods: A total of 126 patients with AMI admitted to our hospital from July to December 2012 were enrolled in this study. All patients underwent echocardiographic examination within 12 hours after admission and received regular follow-ups until December 2018. The primary endpoint was a composite of the major adverse cardiovascular events (MACEs).

Results: In the first year of this study, a primary endpoint occurred in 35 patients and the predictor derived from the echocardiography of 1-year primary endpoint was LVEF<40% (OR: 9.000, 95% CI 3.242-24.987, p<0.0001) and the area under the curve (AUC) for the predictor was 0.676 (95% CI 0.561-0.790, p=0.002). For the total 5 years, 57 patients underwent primary endpoint. The results of the 5-year primary endpoint were: E/E'>15 (OR: 4.094, 95% CI 1.726-9.710, P=0.001), the wall motion score index was (WMSI)>1.5 (OR: 12.791, 95% CI 1.511-108.312, P=0.019), and the AUC was 0.691 (95% CI 0.595-0.787 P<0.0001).

Conclusion: LVEF is correlated with a short-term outcome (1-year), and WMSI and E/E' can predict a long-term outcome (5-year) in patients with acute myocardial infarction.
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http://dx.doi.org/10.2147/IJGM.S304449DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8184283PMC
June 2021

Observer-Based Output Feedback Event-Triggered Adaptive Control for Linear Multiagent Systems Under Switching Topologies.

IEEE Trans Neural Netw Learn Syst 2021 Jun 9;PP. Epub 2021 Jun 9.

The consensus problem of general linear multiagent systems (MASs) is studied under switching topologies by using observer-based event-triggered control method in this article. On the basis of the output information of agents, two kinds of novel event-triggered adaptive control schemes are designed to achieve the leaderless and leader-follower consensus problems, which do not need to utilize the global information of the communication networks. Finally, two simulation examples are introduced to show that the consensus error converges to zero and Zeno behavior is eliminated in MASs. Compared with the existing output feedback control research, one of the significant advantages of our methods is that the controller protocols and triggering mechanisms do not rely on any global information, are independent of the network scale, and are fully distributed ways.
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http://dx.doi.org/10.1109/TNNLS.2021.3084317DOI Listing
June 2021

Cysteine-Based Coupling: Challenges and Solutions.

Bioconjug Chem 2021 Jun 9. Epub 2021 Jun 9.

WuXi Biologics Co, Ltd., Shanghai 200131, China.

Antibody-drug conjugates (ADCs) have attracted great attention in recent years in the wake of an accelerated FDA approval rate and several large-scale acquisitions. To date, there are ten ADC drugs on the market and more than 70 in various stages of clinical trials. Yet, due to the complicated nature of ADC molecules, considerations need to cover many aspects for the success of ADCs, including target specificity, linker-payload stability, tumor permeability, and clearance rate. This topical review summarizes and discusses current methods used to increase stability and homogeneity of ADCs of cysteine conjugation. We believe that they will lead to improvement of efficacy and pharmacokinetics (PK) of ADC drugs.
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http://dx.doi.org/10.1021/acs.bioconjchem.1c00213DOI Listing
June 2021

Simultaneous quantification of fat-soluble vitamins A, 25-hydroxylvitamin D and vitamin E in plasma from children using liquid chromatography coupled to Orbitrap mass spectrometry.

J Chromatogr B Analyt Technol Biomed Life Sci 2021 Jul 29;1177:122795. Epub 2021 May 29.

Center for Clinical Molecular Medicine, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, People's Republic of China.

The fat-soluble vitamins A, D, E and K are micronutrients essential for physiological activity, metabolism and growth. Accurate and sensitive analytical methods are needed to support growing research into fat-soluble vitamins and their impact on children's growth and health. Here we report the first method for simultaneous quantification of fat-soluble vitamins A (retinol), 25-hydroxylvitamin D, 25-hydroxylvitamin D, and vitamin E (α-tocopherol) using a Q-Exactive Orbitrap mass spectrometer in high-resolution, parallel reaction monitoring mode. This method can select desired ions with high efficiency, potentially making it superior to triple-quadrupole mass spectrometers that employ multiple reaction monitoring. The proposed method offers excellent accuracy, specificity, and sensitivity, as demonstrated with plasma samples from healthy children.
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http://dx.doi.org/10.1016/j.jchromb.2021.122795DOI Listing
July 2021

The prevalence of painful physical symptoms in major depressive disorder: A systematic review and meta-analysis of observational studies.

Prog Neuropsychopharmacol Biol Psychiatry 2021 Jun 4:110372. Epub 2021 Jun 4.

Unit of Psychiatry, Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macao SAR, China; Center for Cognition and Brain Sciences, University of Macau, Macao SAR, China; Institute of Advanced Studies in Humanities and Social Sciences, University of Macau, Macao SAR, China. Electronic address:

Background: Painful physical symptoms (PPS) are common in patients with major depressive disorder (MDD), but their prevalence has been mixed. This is a systematic review and meta-analysis of the pooled prevalence of PPS in MDD patients.

Methods: Systematic literature searches were independently conducted in major databases (PubMed, EMBASE, PsycINFO and Web of Science). Data analyses were conducted using a random-effects model.

Results: A total of 20 studies with 53,852 patients were included. The overall prevalence of PPS in MDD patients was 55.2% (95%CI: 47.9-62.3%), with a point prevalence of 64.2% (95% CI: 53.2-73.8%) and a 12-month prevalence of 57.0% (95%CI: 23.9-84.8%). No significant publication bias was found in this meta-analysis.

Conclusion: PPS are common in MDD patients. Considering the negative impact of PPS on daily functioning, effective preventive measures and routine screening should be conducted for MMD patients, and timely treatments should be offered to those in need. Registration number: CRD42020179471.
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http://dx.doi.org/10.1016/j.pnpbp.2021.110372DOI Listing
June 2021

MicroRNA-145-5p inhibits hypoxia/reoxygenation-induced apoptosis in H9c2 cardiomyocytes by targeting ROCK1.

Exp Ther Med 2021 Aug 25;22(2):796. Epub 2021 May 25.

Department of Cardiology, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250033, P.R. China.

There is increasing evidence that microRNAs (miRs) play critical roles in the pathological and physiological processes associated with myocardial ischemia reperfusion (I/R). miR-145 has been extensively studied in the cardiovascular system; however, the role of miR-145 in myocardial I/R remains unclear. Therefore, the present study aimed to investigate the role and mechanism of miR-145-5p in myocardial I/R by establishing a hypoxia/reoxygenation (H/R) model using H9c2 cardiomyocytes. The expression of miR-145-5p was regulated by transfection and the potential target of miR-145-5p was identified. In addition, apoptosis of the cardiomyocytes was evaluated using flow cytometry and the detection of cleaved caspase-3 by western blotting. The results revealed that miR-145-5p expression was decreased while cell apoptosis and Rho-associated coiled-coil-containing kinase 1 (ROCK1) expression were increased in H/R-stimulated H9c2 cardiomyocytes. The upregulation of miR-145-5p reduced apoptosis and the expression of ROCK1 in H/R-stimulated H9c2 cardiomyocytes. Furthermore, the overexpression of ROCK1 significantly attenuated the miR-145-5p-induced reduction of apoptosis following H/R. In conclusion, the present study indicates that the overexpression of miR-145-5p inhibits H/R-induced cardiomyocyte apoptosis by targeting ROCK1.
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http://dx.doi.org/10.3892/etm.2021.10228DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8170661PMC
August 2021

DNAzyme-based colorimetric assay and its application for lipopolysaccharide analysis assisted by oxime chemistry.

Biosens Bioelectron 2021 Oct 31;189:113379. Epub 2021 May 31.

Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai, 200444, PR China. Electronic address:

Herein, for the first time, we propose that the cleavage activity of DNAzyme is accompanied by the release of hydroxyl ions, which can be used for colorimetric assay. Subsequently, we further construct a colorimetric strategy for lipopolysaccharide (LPS) analysis by using this property. Detailly, DNAzyme is split into two fragments separately modified with aldehyde group and hydroxylamine group, which can be linked together through oxime chemistry and the presence of LPS can prevent the formation of oxime bond. The formed whole DNAzyme can mediate the release of hydroxyl ions serving for colorimetric signal output. Taking LPS as model targets, DNAzyme-based colorimetric assay has been successfully constructed. This work not only provides a colorimetric strategy to analyze DNAzyme activity, but also gives a new insight to enrich the versatility of DNAzymes and to enhance their multifunctionality.
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http://dx.doi.org/10.1016/j.bios.2021.113379DOI Listing
October 2021

Retinal vessel density correlates with cognitive function in older adults.

Exp Gerontol 2021 Jun 6;152:111433. Epub 2021 Jun 6.

Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA; Department of Neurology, University of Miami Miller School of Medicine, Miami, FL, USA. Electronic address:

Purpose: We examined the associations between retinal microvascular density, cognition, and physical fitness in healthy older adults with no reported cognitive decline.

Methods: Twenty cognitively normal older adults (age: 70.3 ± 4.6 years) were recruited. Both eyes of each subject were imaged using optical coherence tomography angiography. The vessel densities of the retinal vascular network (RVN), superficial vascular plexus (SVP), and deep vascular plexus (DVP) were measured. Cognitive function was assessed using the Mini-mental state examination (MMSE) and Montreal Cognitive Assessment (MoCA), while physical performance was evaluated using the total work during the YMCA cycle ergometer test (TW-YMCA). Spearman correlations (r) were computed between measures of retinal microvascular density, cognitive function, and physical performance.

Results: The MoCA was significantly correlated to vessel density of SVD (r = 0.53, P = 0.02) but not RVN (r = 0.39, P = 0.09) and DVP (r = 0.02, P = 0.93). MoCA was not correlated with TW-YMCA (r = 0.05, P = 0.83). Retinal microvascular densities were not related to TW-YMCA (r = -0.05-0.18, P > 0.05). Additionally, MMSE was not related the retinal vessel densities (r = -0.10-0.21, P > 0.05) and TW-YMCA (r = -0.19, P = 0.41).

Conclusions: This is the first study to reveal the association between retinal vessel density and cognition as measured with MoCA in healthy older adults with no reported cognitive decline.
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http://dx.doi.org/10.1016/j.exger.2021.111433DOI Listing
June 2021

Gender differences in hematotoxicity of benzene-exposed workers, three cross-sectional studies on 218,061 subjects.

Environ Sci Pollut Res Int 2021 Jun 4. Epub 2021 Jun 4.

Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing, 210009, Jiangsu, China.

Our aim was to analyze the effects of benzene exposure on hematotoxicity in workers, with a focus on gender differences. The study was divided into three parts, and the survey included 218,061 workers. Since 2017, some workers are selected from the total workers each year to explore the possible influencing factors (age, duration of benzene exposure, TWA (8-h time-weighted average) of benzene, SPMA (S-phenylmercapturic acid), MDA (malondialdehyde), 8-OHdG (8-hydroxy-2'-deoxyguanosine) of different hematotoxicity of different genders). The abnormal rate of WBC (white blood cell), ANC (absolute neutrophil count), and platelets of female workers in the benzene exposure group was higher than that of males in the benzene exposure group and also higher than that of the female workers in the control group. Research results in 2019 showed increased SPMA as well as increases their DNA damage including 8-OHdG and MDA in benzene-exposed female workers compared to those in the control female group (all p < 0.05. SPMA, 8-OHdG, and MDA in benzene exposure female workers increased 555%, 183%, and 33.3%, respectively). Female workers are at significantly higher risk for blood system effects of benzene exposure. Therefore, more stringent standards and guidelines may be needed to protect the changing professional population, especially for females.
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http://dx.doi.org/10.1007/s11356-021-14657-0DOI Listing
June 2021

ELK1‑mediated upregulation of lncRNA LBX2‑AS1 facilitates cell proliferation and invasion via regulating miR‑491‑5p/S100A11 axis in colorectal cancer.

Int J Mol Med 2021 Jul 3;48(1). Epub 2021 Jun 3.

Division of Gastroenterology, The Affiliated Huai'an No. 1 People's Hospital of Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China.

The aim of the present study was to investigate the role and regulatory mechanism of LBX2 antisense RNA 1 (LBX2‑AS1) in colorectal cancer. Firstly, LBX2‑AS1 expression was detected using reverse transcription‑quantitative PCR in colorectal cancer tissues and cells, and its prognostic and diagnostic efficacy was assessed in a colorectal cancer cohort (n=145). Subcellular fractionation assay of LBX2‑AS1 was performed. Secondly, the effects of LBX2‑AS1 and microRNA (miR)‑491‑5p on colorectal cancer cell proliferation, apoptosis, migration and invasion were investigated by a series of functional assays. Thirdly, RNA immunoprecipitation, dual‑luciferase reporter and gain and loss of function assays were carried out to analyze the interactions between ETS transcription factor ELK1 (ELK1) and LBX2‑AS1, as well as LBX2‑AS1, miR‑491‑5p and S100A11. The results showed that LBX2‑AS1 was upregulated both in colorectal cancer tissues and cells, which was distributed in the cytoplasm and nucleus of colorectal cancer cells. Clinically, high LBX2‑AS1 expression could be an independent prognostic factor for colorectal cancer. Furthermore, relative operating characteristic curve analysis showed that LBX2‑AS1 was a sensitive diagnostic marker for colorectal cancer. Highly expressed ELK1, as a transcription factor, could bind to the two conserved sites in the promoter region of LBX2‑AS1, thereby activating the transcription of LBX2‑AS1. Silencing LBX2‑AS1 markedly inhibited proliferative, migratory and invasive abilities of colorectal cancer cells. miR‑491‑5p expression was downregulated, while S100A11 expression was upregulated in colorectal cancer tissues and cells. Dual‑luciferase reporter assays confirmed that LBX2‑AS1 could block S100A11 degradation via competitively binding to miR‑491‑5p. Furthermore, LBX2‑AS1 overexpression could notably reverse the inhibitory effect of miR‑491‑5p on proliferation and invasion of colorectal cancer cells. Taken together, LBX2‑AS1 induced by transcription factor ELK1 may facilitate colorectal cancer cell proliferation and invasion via regulation of the miR‑491‑5p/S100A11 axis. Thus, LBX2‑AS1 could be an underlying prognostic and diagnostic marker for colorectal cancer.
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http://dx.doi.org/10.3892/ijmm.2021.4971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175069PMC
July 2021

Stability analysis on dark solitons in quasi-1D Bose-Einstein condensate with three-body interactions.

Sci Rep 2021 May 31;11(1):11382. Epub 2021 May 31.

College of Physics and Electronic Engineering, Northwest Normal University, Lanzhou, 730070, People's Republic of China.

The stability properties of dark solitons in quasi-one-dimensional Bose-Einstein condensate (BEC) loaded in a Jacobian elliptic sine potential with three-body interactions are investigated theoretically. The solitons are obtained by the Newton-Conjugate Gradient method. A stationary cubic-quintic nonlinear Schrödinger equation is derived to describe the profiles of solitons via the multi-scale technique. It is found that the three-body interaction has distinct effect on the stability properties of solitons. Especially, such a nonlinear system supports the so-called dark solitons (kink or bubble), which can be excited not only in the gap, but also in the band. The bubbles are always linearly and dynamically unstable, and they cannot be excited if the three-body interaction is absent. Both stable and unstable kinks, depending on the physical parameters, can be excited in the BEC system.
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http://dx.doi.org/10.1038/s41598-021-90814-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8166838PMC
May 2021

Diagnostic Value of Diurnal Variability of Orthostatic Heart Rate Increment in Children and Adolescents With POTS.

Front Pediatr 2021 13;9:644461. Epub 2021 May 13.

Department of Pediatric Cardiovasology, Children's Medical Center, The Second Xiangya Hospital, Central South University, Changsha, China.

This study aims to investigate the diurnal variability of heart rate (HR) increment after standing (ΔHR) in pediatric postural tachycardia syndrome (POTS) and explore appropriate cutoff values of ΔHR at different times for the POTS diagnosis. Seventy-eight patients (9-14 years) who presented with orthostatic intolerance symptoms were enrolled. Forty-three patients were diagnosed as POTS (ΔHR ≥40 bpm), and 35 patients were assigned to the non-POTS group (ΔHR <40 bpm). Twenty-six healthy children served as the control group. All subjects completed three standing tests in the morning, afternoon, and evening. Orthostatic HR parameters were analyzed to predict the diagnosis of POTS. Additionally, 41 patients were recruited as an external validation group. Orthostatic HR increments in both the POTS and non-POTS groups exhibited diurnal variability, which was markedly larger in the morning ( < 0.05), whereas it did not differ with the time of day in the control group. Among the POTS patients, 100% met the diagnostic criteria for POTS in the morning, 44.2% in the afternoon, and 27.9% in the evening. Almost half of the POTS patients (51.2%) displayed a positive result only in the morning standing test. However, in the three standing tests at different times, ΔHR from 1 to 10 min after standing and ΔHR were the highest in the POTS group compared with in the non-POTS and control groups ( < 0.05). Furthermore, the maximum ΔHR (ΔHR) and ΔHR at 5 and 10 min in the afternoon and evening standing tests yielded moderate predictive values for the POTS diagnosis. The external validation test showed that the afternoon ΔHR ≥30 bpm to diagnose POTS yielded sensitivity, specificity, and accuracy of 85, 71.4, and 78%, respectively, and the evening ΔHR ≥25 bpm yielded sensitivity, specificity, and accuracy of 85, 76.2, and 80.5%, respectively. The orthostatic HR increment exhibits diurnal variability in children and adolescents with POTS that may affect the diagnosis of POTS. Supplementary criteria are proposed for the POTS diagnosis based on diurnal variability.
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http://dx.doi.org/10.3389/fped.2021.644461DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8157922PMC
May 2021

Decrease of ABCB1 protein expression and increase of G phase arrest induced by oleanolic acid in human multidrug-resistant cancer cells.

Exp Ther Med 2021 Jul 9;22(1):735. Epub 2021 May 9.

Institute of Medicinal Biotechnology, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, P.R. China.

Oleanolic acid (OA) is a natural compound that can be found in a number of edible and medicinal plants and confers diverse biological actions. However, the direct target of OA in human tumor cells remains poorly understood, preventing its application in clinical and health settings. A previous study revealed that overexpression of caveolin-1 in human leukemia HL-60 cells can increase its sensitivity to OA. The present study aimed to investigate the effects of OA on the doxorubicin-resistant human breast cancer MCF-7 cell line (MCF-7/DOX), harringtonine-resistant human leukemia HL-60 cells (HL-60/HAR) and their corresponding parental cell lines. Western blotting was performed to measure protein expression levels, whilst Cell Counting Kit-8 (CCK-8) assays, cell cycle analysis (by flow cytometry) and apoptosis assays (with Annexin V/PI staining) were used to assess drug sensitivity. CCK-8 assay results suggested that MCF-7/DOX cells, which overexpress the caveolin-1 protein, have similar OA susceptibility to their parent line. In addition, sensitivity of MCF-7/DOX cells to OA was not augmented by knocking down caveolin-1 using RNA interference. HL-60/HAR cells exhibited a four-fold increased sensitivity to OA compared with that in their parental HL-60 cells according to CCK-8 assay. Both of the resistant cell lines exhibited higher numbers of cells at G phase arrest compared with those in their parent lines, as measured via flow cytometry. Treatment of both MCF-7 cell lines with 100 µM OA for 48 h induced apoptosis, with increased effects observed in resistant cells. However, no PARP-1 or caspase-3 cleavage was observed, with some positive Annexin V staining found after HL-60/HAR cells were treated with OA, suggesting that cell death occurred via non-classical apoptosis or through other cell death pathways. It was found that OA was not a substrate of ATP-binding cassette subfamily B member 1 (ABCB1) in drug-resistant cells, as indicated by the accumulation of rhodamine 123 assessed using flow cytometry. However, protein expression of ABCB1 in both of the resistant cell lines was significantly decreased after treatment with OA in a concentration-dependent manner. Collectively, these results suggest that OA could reduce ABCB1 protein expression and induce G phase arrest in multidrug-resistant cancer cells. These findings highlight the potential of OA for cancer therapy.
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http://dx.doi.org/10.3892/etm.2021.10167DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138263PMC
July 2021

Hierarchical Hydrophilic/Hydrophobic/Bumpy Janus Membrane Fabricated by Femtosecond Laser Ablation for Highly Efficient Fog Harvesting.

ACS Appl Mater Interfaces 2021 Jun 27;13(22):26542-26550. Epub 2021 May 27.

CAS Key Laboratory of Mechanical Behavior and Design of Materials, Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei 230026, China.

The shortage of freshwater is threatening sustainable economic development and ecological security worldwide. Janus membrane, as a highly efficient method to collect the invisible fog water in the wet environment, is still hindered by some inherent limitations: (1) poor condensation of fog droplets on the superhydrophobic side due to the ultralow adhesive force of droplets with substrate and (2) insufficient detachment of droplets from the superhydrophilic side in time, which hampers the continuous water transport in the micropores. Herein, inspired by the desert beetle's back with alternating hydrophobic/hydrophilic bumps and the cactus thorn with an asymmetric geometry, we design and fabricate a kind of hierarchical hydrophilic/hydrophobic/bumpy Janus (HHHBJ) membrane by femtosecond laser ablation on an aluminum membrane to achieve the self-driven fog collection, which achieves over 250% enhancement in the water collection efficiency over the conventional Janus membrane. Even when the mist flow is applied to the surface at an incident angle of 45°, the collection efficiency increases by 600%. The mechanism of the HHHBJ film with excellent fog collection efficiency is mainly related to the continuous efficient fog condensation on the top surface and droplet removal on the bottom surface in time. We believe the proposed multi-bioinspired HHHBJ film with droplet self-driven collection ability provides insights to conceive and construct a highly efficient fog collection system in broad fields.
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http://dx.doi.org/10.1021/acsami.1c02121DOI Listing
June 2021

Effectiveness of acupuncture therapy for postherpetic neuralgia: an umbrella review protocol.

BMJ Open 2021 05 21;11(5):e043064. Epub 2021 May 21.

Department of Pain, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, China.

Introduction: Several systematic reviews and meta-analysis indicate that acupuncture and related therapies may be a valuable adjunctive technique to pharmacological interventions for pain management of postherpetic neuralgia (PHN). However, the robustness of the results of these studies has not been evaluated. The aim of this proposed umbrella review is to provide more reliable evidence of the effectiveness of acupuncture therapy for PHN based on medical references for healthcare decision makers.

Methods And Analysis: PubMed, EMBASE, The Cochrane Library, Web of Science, Chinese BioMedical Literature Database, VIP Database for Chinese Technical Periodicals, China National Knowledge Infrastructure and Wan fang Database will be used to retrieve reviews. The time of publication will be limited from inception to March 2021. Two reviewers will screen all retrieved articles independently to identify their eligibility and extract the data. The quality will be assessed independently by two trained reviewers using Assessment of Multiple Systematic Reviews-2 for methodological quality, Risk of Bias in Systematic Review for level of bias, Preferred Reporting Items for Systematic Reviews and Meta-Analysis for reporting quality and Grading of Recommendations Assessment, Development and Evaluation for the quality of evidence. Any disagreements will be settled by discussion or the involvement of a third reviewer.

Ethics And Dissemination: The protocol of this review does not require ethical approval because the research will be based on publicly available data. The findings will be disseminated through publication in peer-reviewed international journals or presentation in academic conference.

Prospero Registration Number: CRD42020173341.

Reporting Checklist: PRISMA-P, 2015.
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http://dx.doi.org/10.1136/bmjopen-2020-043064DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144037PMC
May 2021

Performance of activated carbons prepared from spent tyres in the adsorption of rhodamine B in aqueous solutions.

Environ Sci Pollut Res Int 2021 May 21. Epub 2021 May 21.

Centre for Energy (M473), The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia.

Activated carbons were produced from spent tyre pyrolysis char by steam or CO activation and evaluated for their performance in rhodamine B (RhB) adsorption in aqueous solutions. The effect of RhB starting concentration (80-150 mg L), contact time (0-80 min), temperature (298-318 K) and initial pH on the adsorption process was examined. Pseudo-first-order and pseudo-second-order models were carried out to fit the experimental data to derive RhB adsorption kinetics. Langmuir, Freundlich and Temkin isotherm models were applied to depict RhB adsorption behaviour of the prepared activated carbons. Gibbs free energy (ΔG), enthalpy (ΔH) and entropy (ΔS) were calculated. It has been found that the activated carbons can effectively adsorb RhB due to high mesoporosity and RhB equilibrium adsorption capacity (q) increased almost linearly with increasing total mesopore volumes, regardless of the activation agents. When BET surface areas are similar, CO-activated carbon obtained higher q than steam due to higher mesoporosity of CO-activated carbon. The results show that pseudo-second-order well fitted the experimental data. RhB starting concentration increased from 80 to 150 mg L causing q increased from 158 to 251 mg g but RhB removal decreased from 99.7 to 84.5%. The RhB adsorption process follows the Langmuir model and thermodynamic calculation, indicating RhB adsorption is an endothermic, spontaneous process, dominated by both chemisorption and physisorption.
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http://dx.doi.org/10.1007/s11356-021-14502-4DOI Listing
May 2021

The efficacy and safety of Nivolumab combined with Ipilimumab in the immunotherapy of cancer: a meta-analysis.

Immunopharmacol Immunotoxicol 2021 Jun 20;43(3):386-394. Epub 2021 May 20.

Antibody Engineering Laboratory, School of Life Science & Technology, China Pharmaceutical University, Nanjing, PR China.

Nivolumab and Ipilimumab are immune checkpoint inhibitors. The combination of Nivolumab and Ipilimumab has been reported to have complementary effects in the treatment of metastatic melanoma. The combination therapy of Nivolumab and Ipilimumab (N+I) has shown synergistic effects in cancer immunotherapy but this is still controversial due to the higher incidence of toxicity. Hence, we conducted a meta-analysis to evaluate the efficacy and safety profile of Nivolumab combined with Ipilimumab and compared the different dosing schedules of the N+I combination. By searching in PubMed, PMC, Cochrane library and major conference abstracts, eligible sixteen studies including N+I therapy and Nivolumab monotherapy were selected to analyze overall response rate (ORR), progression-free survival (PFS), overall survival (OS), and high-grade (3-4) adverse effects (AEs). Compared with monotherapy of Nivolumab, N+I significantly improved ORR (RR=1.40 [95% CI 1.27, 1.54], P<0.00001) and PFS (Hazard Ratio (HR)=0.83 [95% CI 0.77, 0.90], P<0.00001), but not OS (HR=0.93 [95% CI 0.84, 1.03], P=0.16). In a sub-analysis, the combination of Nivolumab 1mg/kg plus Ipilimumab 3mg/kg (N1I3) and Nivolumab 3mg/kg plus Ipilimumab 1mg/kg (N3I1) achieved better ORR and PFS than Nivolumab 3mg/kg (N3) alone. Remarkably, OS was also prolonged with the N1I3 combination compared with the N3I1 combination or N3. Furthermore, a higher incidence of high-grade AEs also occurred with the combination therapy of N1I3. N+I combination therapy showed greater ORR and PFS compared with Nivolumab monotherapy. N1I3 combination provided the benefit of ORR, PFS and OS but was associated with a higher incidence of toxicity.
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http://dx.doi.org/10.1080/08923973.2021.1924195DOI Listing
June 2021

Applications of Single-Cell Sequencing in Dermatology.

Med Sci Monit 2021 May 20;27:e931862. Epub 2021 May 20.

Department of Dermatology, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China (mainland).

Single-cell sequencing (SCS) is a promising new technique used to assess the genomics, transcriptomics, epigenetics, and other multi-omics at the single-cell level. In addition to elucidating the immune microenvironment and revealing the pathomechanisms of disease and drug resistance, SCS can profile the actual state of an individual cell and identify a novel cell type and differentiation trajectories, which cannot be achieved by bulk tissue sequencing technique. SCS technique serves as powerful tools to explore more meaningful biomarkers of diagnosis, prognosis, and new therapeutic targets in clinical practice. The SCS technique has been widely applied in the field of dermatology. In this review, we summarize the advances of SCS in dermatology.
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http://dx.doi.org/10.12659/MSM.931862DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8147034PMC
May 2021

Autophagy induced by a novel triazol derivative promotes angiogenesis via decreasing interferon-inducible protein 10 level in vascular endothelial cells.

J Cardiovasc Pharmacol 2021 Apr 17. Epub 2021 Apr 17.

School of Biological Science and Technology, University of Jinan, Jinan 250022, China. Shandong Provincial Hospital Affiliated to Shandong University.

Abstract: Autophagy plays an important role in angiogenesis, while the mechanisms of vascular endothelial cell (VEC) autophagy associated with angiogenesis remain unclear. In this study, we identified a novel triazol derivative (JL025) that significantly promoted angiogenesis both in vitro and in vivo. Moreover, JL025 had no effects on cell proliferation but dramatically increased the autophagy level of VEC. The suppression of autophagy inhibited JL025-induced angiogenesis in vitro and in vivo, suggesting that JL025-induced angiogenesis was dependent on the enhanced autophagy. Mechanistic studies indicated that JL025-induced VEC autophagy was related to the Akt/mTOR signaling pathway. Meanwhile, JL025 decreased the anti-angiogenic chemokine interferon-inducible protein 10 (IP10) protein level in HUVECs. Importantly, suppression of autophagy inhibited JL025-induced decrease of IP10 protein level, indicating that autophagy mediated the degradation of IP10. Taken together, our findings provide new insights into the relationship of VEC autophagy with angiogenesis, and JL025 may have a therapeutic potential in related diseases.
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http://dx.doi.org/10.1097/FJC.0000000000001034DOI Listing
April 2021

Conferring thermotolerant phenotype to wild-type Yarrowia lipolytica improves cell growth and erythritol production.

Biotechnol Bioeng 2021 May 19. Epub 2021 May 19.

School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China.

In microbial engineering, heat stress is an important environmental factor modulating cell growth, metabolic flux distribution and the synthesis of target products. Yarrowia lipolytica, as a GARS (generally recognized as safe) nonconventional yeast, has been widely used in the food industry, especially as the host of erythritol production. Biomanufacturing economics is limited by the high operational cost of cooling energy in large-scale fermentation. It is of great significance to select thermotolerant Y. lipolytica to reduce the cooling cost and elucidate the heat-resistant mechanism at molecular level. For this purpose, we performed adaptive evolution and obtained a thermotolerant strain named Y. lipolytica BBE-18. Transcriptome analysis allows us to identify four genes in thiamine metabolism pathway that are responsible for the complicated thermotolerant phenotype. The heat-resistant phenotype was validated with the model strain Y. lipolytica Po1f by overexpression of single and combined genes. Then, conferring the thermotolerant phenotype to the wild-type Y. lipolytica BBE-17 enable the strain to produce three-times more erythritol of the control strain with 3°C higher than optimal cultivation temperature. To our knowledge, this is the first report on engineering heat-resistant phenotype to improve the erythritol production in Y. lipolytica. However, due to the increase of culture temperature, a large amount of adenosine triphosphate is consumed to ensure the life activities of Y. lipolytica which limits the potential of cell synthetic products to a certain extent. Even so, this study provides a reference for Y. lipolytica to produce other products under high temperature.
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http://dx.doi.org/10.1002/bit.27835DOI Listing
May 2021

Nomogram for predicting preoperative regional lymph nodes metastasis in patients with metaplastic breast cancer: a SEER population-based study.

BMC Cancer 2021 May 17;21(1):565. Epub 2021 May 17.

Department of Medical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, No 277 Yanta West Road, Xi'an, Shaanxi, 710061, People's Republic of China.

Background: Metaplastic breast cancer (MBC) is a rare subtype of breast cancer, and generally associated with poor outcomes. Lymph nodes metastasis (LNM) is confirmed as a critical independent prognostic factor and determine the optimal treatment strategies in MBC patients. We aimed to develop and validate a nomogram to predict the possibility of preoperative regional LNM in MBC patients.

Methods: MBC patients diagnosed between 1990 and 2016 in the Surveillance, Epidemiology, and End Results (SEER) database were included and stochastically divided into a training set and validation set at a ratio of 7:3. The risk variables of regional LNM in the training set were determined by univariate and multivariate logistic regression analyses. And then we integrated those risk factors to construct the nomogram. The prediction nomogram was further verified in the verification set. The discrimination, calibration and clinical utility of the nomogram were evaluated by the area under the receiver operating characteristic (ROC) curve (AUC), calibration plots and decision curve analysis (DCA), respectively.

Results: A total of 2205 female MBC patients were included in the study. Among the 2205 patients, 24.8% (546/2205) had positive regional lymph nodes. The nomogram for predicting the risk of regional LNM contained predictors of grade, estrogen receptor (ER) status and tumor size, with AUC of 0.683 (95% confidence interval (CI): 0.653-0.713) and 0.667 (95% CI: 0.621-0.712) in the training and validation sets, respectively. Calibration plots showed perfect agreement between actual and predicted regional LNM risks. At the same time, DCA of the nomogram demonstrated good clinical utilities.

Conclusions: The nomogram established in this study showed excellent prediction ability, and could be used to preoperatively estimate the regional LNM risk in MBC.
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http://dx.doi.org/10.1186/s12885-021-08313-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130108PMC
May 2021

HnRNP F and hnRNP H1 regulate mRNA stability of amyloid precursor protein.

Neuroreport 2021 Jun;32(9):824-832

Institute on Aging and Brain Disorders, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences.

Amyloid precursor protein (APP) is a transmembrane protein that plays a crucial role in the production of amyloid-β peptides. Any disruption in APP protein production, its mRNA decay rate or processing may result in abnormal production of amyloid-β peptides and subsequent development of protein aggregation diseases. Therefore, the equilibrium is crucial for neuronal function. An association study of heterogeneous nuclear ribonucleoprotein (hnRNP)-F and hnRNP H1 with APP was carried out in Neuro-2a (N2a) cells. In the present study, we found that hnRNP F and hnRNP H1 were significantly upregulated in the hippocampus of APP/PS1 mice. The changes in APP expression were positively associated with hnRNP F and hnRNP H1 when hnRNP F and hnRNP H1 were depleted or increased in N2a cells. Importantly, cross-linked RNA immunoprecipitation demonstrated binding affinities of hnRNP F and hnRNP H1 for App mRNA. Mechanistically, mRNA stability assay revealed that overexpression of hnRNP F or hnRNP H1 increases the APP level by stabilizing App mRNA half-life, implying that levels of hnRNP F and hnRNP H1 can change the production of APP. Further understanding of the regulatory mechanism of APP expression in association with hnRNP F and hnRNP H1 would provide insights into the mechanism underlying the maintenance of brain health and cognition. This study provides a theoretical basis for the development of hnRNP-stabilizing compounds to regulate APP.
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http://dx.doi.org/10.1097/WNR.0000000000001662DOI Listing
June 2021

Heterotrophic nitrification and related functional gene expression characteristics of Alcaligenes faecalis SDU20 with the potential use in swine wastewater treatment.

Bioprocess Biosyst Eng 2021 May 12. Epub 2021 May 12.

State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, 266237, People's Republic of China.

A new heterotrophic nitrifying bacterium was isolated from the compost of swine manure and rice husk and identified as Alcaligenes faecalis SDU20. Strain SDU20 had heterotrophic nitrification potential and could remove 99.7% of the initial NH-N. Nitrogen balance analysis revealed that 15.9 and 12.3% of the NH-N were converted into biological nitrogen and nitrate nitrogen, respectively. The remaining 71.44% could be converted into N or NO. Single-factor experiments showed that the optimal conditions for ammonium removal were the carbon source of sodium succinate, C/N ratio 10, initial pH 8.0, and temperature 30 °C. Nitrification genes were determined to be upregulated when sodium succinate was used as the carbon source analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Strain SDU20 could tolerate 4% salinity and show resistance to some heavy metal ions. Strain SDU20 removed 72.6% high concentrated NH-N of 2000 mg/L within 216 h. In a batch experiment, the highest NH-N removal efficiency of 98.7% and COD removal efficiency of 93.7% were obtained in the treatment of unsterilized swine wastewater. Strain SDU20 is promising in high-ammonium wastewater treatment.
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http://dx.doi.org/10.1007/s00449-021-02581-zDOI Listing
May 2021

Exogenous application of brassinosteroids regulates tobacco leaf size and expansion via modulation of endogenous hormones content and gene expression.

Physiol Mol Biol Plants 2021 Apr 27;27(4):847-860. Epub 2021 Mar 27.

Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Tobacco Biology and Processing, Ministry of Agriculture and Rural Affairs, Qingdao, 266101 China.

Brassinosteroids (BR) play diverse roles in the regulation of plant growth and development. BR promotes plant growth by triggering cell division and expansion. However, the effect of exogenous BR application on the leaf size and expansion of tobacco is unknown. Tobacco seedlings are treated with different concentrations of exogenous 2,4-epibrassinolide (EBL) [control (CK, 0 mol L), T1 (0.5 × 10 mol L), and T2 (0.5 × 10 mol L)]. The results show that T1 has 17.29% and T2 has 25.99% more leaf area than control. The epidermal cell area is increased by 24.40% and 17.13% while the number of epidermal cells is 7.06% and 21.06% higher in T1 and T2, respectively, relative to control. So the exogenous EBL application improves the leaf area by increasing cell numbers and cell area. The endogenous BR (7.5 times and 68.4 times), auxin (IAA) (4.03% and 25.29%), and gibberellin (GA) contents (84.42% and 91.76%) are higher in T1 and T2, respectively, in comparison with control. Additionally, , , and are upregulated showing that the brassinosteroid signaling pathway is activated. Furthermore, the expression of the key biosynthesis-related genes of BR (), IAA (), and GA () are all upregulated under EBL application. Finally, the exogenous EBL application also upregulated the expression of cell growth-related genes (, , , , and ). The results reveal that the EBL application increases the leaf size and expansion by promoting the cell expansion and division through higher BR, IAA, and GA contents along with the upregulation of cell growth-related genes. The results of the study provide a scientific basis for the effect of EBL on tobacco leaf growth at morphological, anatomical, biochemical, and molecular levels.

Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-021-00971-x.
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http://dx.doi.org/10.1007/s12298-021-00971-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055801PMC
April 2021

Preliminary study on impacts of polystyrene microplastics on the hematological system and gene expression in bone marrow cells of mice.

Ecotoxicol Environ Saf 2021 May 4;218:112296. Epub 2021 May 4.

Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China. Electronic address:

Microplastics (MPs) are currently a global environmental pollutants and health hazards that caused by MPs cannot be ignored. However, studies on MP toxicity in mammals are scare. Here, we investigated the effects of two doses (0.1 mg and 0.5 mg) of 5 µm polystyrene microplastic (PS-MP) particles on the hematological system of mice through traditional toxicology experiments and assessed the related potential biological mechanisms using transcriptome sequencing analysis. The toxicological examinations showed that the 0.5 mg dose significantly decreased white blood cell count, increased Pit count, and inhibited the growth of colony-forming unit CFU-G, CFU-M and CFU-GM. Compared with the control group, there were 41 differentially expressed genes (DEGs) in the 0.1 mg-treated group and 32 significantly changed genes in 0.5 mg-treated group. Of note, eight genes were found to be significantly altered in both the PS-MP-treated groups. Gene ontology analysis showed that DEGs were mainly involved in T cell homeostasis, response to osmotic stress, extracellular matrix and structure organization, and metabolic process of NADP and nucleotides. In addition, pathway analysis revealed that the Jak/Stat pathway, pentose and glucuronate interconversions, nicotinate and nicotinamide metabolism, biosynthesis of unsaturated fatty acids, and the pentose phosphate pathway were involved in PS-MP-induced toxicity in mice. These results indicated that PS-MP exposure can cause hematotoxicity to some extent, impact gene expression, and disturb related molecular and biological pathways in mouse bone marrow cells. Our study provides fundamental data on the hematotoxicity of PS-MPs in terrestrial mammals that will help to further assess the corresponding health risks in these mammals.
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http://dx.doi.org/10.1016/j.ecoenv.2021.112296DOI Listing
May 2021

MicroRNA-26 regulates the expression of CTGF after exposure to ionizing radiation.

Radiat Environ Biophys 2021 May 6. Epub 2021 May 6.

Department of Matrix Biology and Medicine, Faculty of Medicine, Oita University, Oita, Japan.

Radiation-induced fibrosis (RIF) is a serious complication that occurs after irradiation and which is caused by the deposition of extracellular matrix (ECM) proteins such as collagen. However, the underlying mechanisms, including the expression of the cytokines, that promote the RIF process, are not yet fully understood. MicroRNAs (miRNAs) have recently been suggested to act as post-transcriptional repressors for many genes; however, their role in the process of RIF remains to be elucidated. Our previous study showed that ionizing radiation increased the type I collagen expression through the activation of transforming growth factor (TGF)-β, while miR-29 repressed this increase. This study aimed to investigate the mechanisms by which the expression of connective tissue growth factor (CTGF), a downstream mediator of TGF-β, is controlled by miRNAs post-transcriptionally after exposure to ionizing radiation. The expression of CTGF in NIH-3T3 cells and mouse embryonic fibroblasts was increased by ionizing radiation. However, this increase was suppressed with a specific inhibitor of TGF-β receptor. Among the predictable miRNAs that target the CTGF gene, the expression of miR-26a was downregulated after exposure to ionizing radiation and this regulation was negatively mediated by TGF-β signaling. miR-26a negatively regulated the CTGF expression at the post-transcriptional level; however, ionizing radiation suppressed this negative regulation. In addition, the overexpression of miR-26a inhibited the expression of CTGF and type I collagen after irradiation. In conclusion, miR-26a modulates the expression of CTGF via TGF-β signaling in irradiated fibroblasts. The results suggest the potential application of miR-26a in the treatment of RIF.
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http://dx.doi.org/10.1007/s00411-021-00915-9DOI Listing
May 2021

Formulating the Electrolyte Towards High-Energy and Safe Rechargeable Lithium-Metal Batteries.

Angew Chem Int Ed Engl 2021 May 6. Epub 2021 May 6.

CAS Key Laboratory of Molecular Nanostructure and Nanotechnology, CAS Research/Education Center for Excellence in Molecular Sciences, Beijing National Laboratory for Molecular Sciences (BNLMS), Institute of Chemistry, Chinese Academy of Sciences (CAS), Beijing, 100190, P. R. China.

Rechargeable lithium-metal batteries with a cell-level specific energy of >400 Wh kg are highly desired for next-generation storage applications, yet the research has been retarded by poor electrolyte-electrode compatibility and rigorous safety concerns. We demonstrate that by simply formulating the composition of conventional electrolytes, a hybrid electrolyte was constructed to ensure high (electro)chemical and thermal stability with both the Li-metal anode and the nickel-rich layered oxide cathodes. By employing the new electrolyte, Li∥LiNi Co Mn O cells show favorable cycling and rate performance, and a 10 Ah Li∥LiNi Co Mn O pouch cell demonstrates a practical specific energy of >450 Wh kg . Our findings shed light on reasonable design principles for electrolyte and electrode/electrolyte interfaces toward practical realization of high-energy rechargeable batteries.
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http://dx.doi.org/10.1002/anie.202103850DOI Listing
May 2021

Analysis of carbon emission performance and regional differences in China's eight economic regions: Based on the super-efficiency SBM model and the Theil index.

PLoS One 2021 5;16(5):e0250994. Epub 2021 May 5.

College of Architectural Engineering, Qingdao Binhai University, Qingdao, China.

China's carbon emission performance has significant regional heterogeneity. Identified the sources of carbon emission performance differences and the influence of various driving factors in China's eight economic regions accurately is the premise for realizing China's carbon emission reduction goals. Based on the provincial panel data from 2005 to 2017, the super-efficiency SBM model and Malmquist model are constructed in this paper to measure regional carbon emission performance's static and dynamic changes. After that, the Theil index is used to distinguish the impact of inter-regional and intra-regional differences on different regions' carbon emissions performance. Finally, by introducing the Tobit model, the effect of various driving factors on carbon emission performance differences is analyzed quantitatively. The results show that: (1) There are significant differences in different regions' carbon emission performance, but the overall carbon emission performance presents an upward fluctuation trend. Malmquist index decomposition results show substantial differences in technology progress index and technology efficiency index in different regions, leading to significant carbon emission performance differences. (2) Overall, inter-regional differences contribute the most to the overall carbon emission performance, up to more than 80%. Among them, the inter-regional and intra-regional differences in ERMRYR contributed significantly. (3) Through Tobit regression analysis, it is found that residents' living standards, urbanization level, ecological development degree, and industrial structure positively affect carbon emission performance. On the contrary, energy intensity presents an apparent negative correlation on carbon emission performance. Therefore, to improve the carbon emission performance, we should put forward targeted suggestions according to the characteristics of different regional development stages, regional carbon emission differences, and influencing driving factors.
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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0250994PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8099138PMC
May 2021