Publications by authors named "Juan Wei"

139 Publications

Analysis of the application of "psycho-cardiology" model in nursing care of acute stroke patients with depression.

Am J Transl Res 2021 15;13(7):8021-8030. Epub 2021 Jul 15.

Department of Nursing, Zhujiang Hospital of Southern Medical University Guangzhou, Guangdong Province, China.

Objective: To evaluate the effect of "psycho-cardiology" model in nursing care of acute stroke patients with depression.

Methods: Seventy-eight acute stroke patients with depression were selected for this prospective study, and they were divided into two groups according to the random number table method. The control group (n=39) were given usual care, and the study group (n=39) were given nursing intervention of "psycho-cardiology" model in addition to usual care. The changes of mental state (Hamilton Depression Scale, HAMD; Hamilton Anxiety Scale, HAMA), the neurological function (National Institute of Health Stroke scale, NIHSS), and the cognitive function (Mini-Mental State Examination, MMSE), the prognostic indicator (Fugl-Meyer Assessment, FMA; Barthel Index, BI) were compared between the two groups before and after the intervention. The incidence of complications and nursing satisfaction were also compared between the two groups.

Results: After nursing, the scores of HAMA and HAMD in the study group were significantly lower than those in the control group (P<0.05). The NIHSS score of the study group was significantly lower than that of the control group (P<0.05). The score of MMSE in the study group was significantly higher than that of the control group (P<0.05). The scores of FMA and BI in the study group were significantly higher than those of the control group (P<0.05). There was no significant difference in the incidence of complications between the two groups (P>0.05). The nursing satisfaction of the study group was significantly higher than that of the control group (P<0.05).

Conclusion: Nursing intervention of "psycho-cardiology" model for acute stroke patients with depression can effectively alleviate the mental stress of patients, improve neurological function and cognitive function, reduce the occurrence of complications, improve prognosis and nursing satisfaction.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8340265PMC
July 2021

Template-free multiple signal amplification for highly sensitive detection of cancer cell-derived exosomes.

Chem Commun (Camb) 2021 Sep 5;57(68):8508-8511. Epub 2021 Aug 5.

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, P. R. China.

In this work, we have designed a template-free multiple signal amplification method for the highly sensitive detection of cancer cell-derived exosomes. In this design, DNase I serves as a bridge to link the DNA-based amplification approach and terminal deoxynucleotidyl transferase (TdT)-mediated polymerization reaction. Consequently, a detection limit of 10 particles per μL can be achieved, while a complex nucleic acid sequence design can be avoided. This method also exhibits good performance in a complicated matrix and enables the differentiation of healthy individuals from colorectal cancer (CRC) patients.
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http://dx.doi.org/10.1039/d1cc03640hDOI Listing
September 2021

Central venous catheterization site choice based on anatomical landmark technique: a systematic review and meta-analysis.

J Anesth 2021 Aug 2. Epub 2021 Aug 2.

Department of Anesthesiology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, 507 Zhengmin Rd, Shanghai, China.

Background: Internal jugular vein catheterization (IJVC) and subclavian vein catheterization (SCVC) have been the most preferred central venous catheterizations (CVC) clinically. Individual preference and institutional routine dominate the traditional CVC choice; however, it is lack of high-level evidence. We sought to provide better clinical strategy for CVC site choice based on anatomical landmark technique between IJVC and SCVC.

Methods: We systematically reviewed eligible studies from PubMed, OVID, Cochrane and ClinicalTrials.Gov till February 2020. The primary outcomes were catheterization time and overall success rate, and the secondary outcomes were the first-attempt success rate and the instant mechanical complications. Ethical problems are not applicable.

Results: A total of 3378 patients from 7 studies were included in the analyses. Neither difference was found on the catheterization time (SMD 95% CI: -0.095-0.124, p = 0.792), nor any difference on the overall success rate (RR = 1.017, 95% CI: 0.927-1.117, p = 0.721, I = 89.6%) between the 2 procedures. However, subgroup analyses showed overall success rate of IJVC was significantly lower than that of SCVC (RR = 0.906, 95% CI: 0.850-0.965, p = 0.002) in adults. The first-attempt success rate of IJVC group was higher in the adults (RR = 1.472, 95% CI: 1.004-2.156, p = 0.047). No significance was detected in arterial injury (RR = 1.137, 95% CI: 0.541-2.387, p = 0.735) and pneumothorax (RR = 0.600, 95% CI: 0.32-1.126, p = 0.112) between the two procedures. Hematoma was significantly more in IJVC group than that in SCVC group (RR = 2.824, 95% CI: 1.181-6.751, p = 0.02).

Conclusions: Compared with IJVC, SCVC shows a higher overall success rate while a lower first-attempt success rate in adults, and has involved with less hematoma.

Prospero Registration: CRD42020165444.
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http://dx.doi.org/10.1007/s00540-021-02976-yDOI Listing
August 2021

Adsorption of Hg(II) in solution by mercaptofunctionalized palygorskite.

Environ Sci Pollut Res Int 2021 Jul 31. Epub 2021 Jul 31.

Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials, Nanjing University of Posts and Telecommunications, No. 9 Wenyuan Road, Yadong New District, Nanjing, 210023, China.

In the past 10 years, the treatment and restoration of soil and water bodies contaminated by mercury and other heavy metals have received unprecedented attention and support from China's environmental protection authorities. The search for low-cost and high-efficiency adsorbents has become one of the research hotspots in this field. In this paper, a simple and environment-friendly method was used to graft 3-mercaptopropyl trimethoxysilane on the surface of palygorskite. The synthesized mercaptofunctionalized palygorskite (M-PAL) was characterized by XRD, FT-IR, BET and SEM-EDS, respectively, and its adsorption conditions, adsorption models and thermodynamic parameters for Hg were systematically investigated. The experimental results indicated that the saturated adsorption capacity of Hg on the M-PAL could reach 203.4 mg·g, within 120 min at pH 4 and 298 K. By analyzing the experimental data of adsorption kinetics and thermodynamics, it was found that the adsorption process of Hg conformed to the pseudo-second-order kinetic model, which belonged to chemical adsorption of the rate-controlled step; the Langmuir model better described the adsorption isotherm. The thermodynamic parameters obtained (ΔH=29.95 kJ·mol, ΔS=103.09 J·mol·K and ΔG<0) show that the whole process is a spontaneous endothermic process. When the concentration of Na, K, Ca, Mg, Cl, NO, HCO and CHO was 200 times that of Hg, although these organic acids had a slightly greater effect on the adsorption of Hg on mercaptofunctionalized palygorskite than inorganic ions, the adsorption capacity remained above 185 mg·g. The adsorption products could be still stable in simulated acid rain with pH 3, 4, 5, 6, 7 and oxalic acid solution with concentration of 1, 2, 3, 4 and 5 mmol·L, and the desorption rates were about 3%. Through XPS analysis, the specific coordination of Hg with the S atom on the surface of M-PAL was confirmed.
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http://dx.doi.org/10.1007/s11356-021-15637-0DOI Listing
July 2021

Altered Cerebral Blood Flow in Alzheimer's Disease With Depression.

Front Psychiatry 2021 8;12:687739. Epub 2021 Jul 8.

Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

Depression is common in Alzheimer's disease (AD) with an unclear neural mechanism. This study aimed to investigate the underlying cerebral perfusion associated with depression in AD and evaluate its clinical significance. Twenty-one AD patients and 21 healthy controls (HCs) were enrolled in this study. The depressive symptom was defined according to the Hamilton Depression Rating Scale (HAMD). Nine patients were diagnosed as AD with depression symptoms (HAMD >7). Three-dimensional pseudocontinuous arterial spin labeling MR imaging was conducted to measure regional cerebral blood flow (CBF). Neuropsychological tests covered cognition and depressive scores. Between-group comparisons on clinical variables and regional CBFs, relationship between regional CBF and depressive score, and identification of AD patients with depression were performed using covariance analysis, linear regression, and receiver operating characteristic (ROC) analysis, respectively. Compared with HCs, AD patients without depression exhibited lower gray matter CBF ( = 0.016); compared with AD patients without depression, AD patients with depression had higher CBF in the right supplementary motor area (39.23 vs. 47.91 ml/100 g/min, = 0.017) and right supramarginal gyrus (35.54 vs. 43.85 ml/100 g/min, = 0.034). CBF in the right supplementary motor area was correlated with depressive score (β = 0.46, = 0.025). The combination of CBF in the right supplementary motor area and supramarginal gyrus and age could identify AD patients with depression from those without depression with a specificity of 100%, sensitivity of 66.67%, accuracy of 85.71%, and area under the curve of 0.87. Our findings suggested that hyperperfusion of the right supplementary motor area and right supramarginal gyrus were associated with depression syndrome in AD, which could provide a potential neuroimaging marker to evaluate the depression state in AD.
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http://dx.doi.org/10.3389/fpsyt.2021.687739DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8295555PMC
July 2021

Author Correction: The plant ESCRT component FREE1 shuttles to the nucleus to attenuate abscisic acid signalling.

Nat Plants 2021 Aug;7(8):1160

Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University (SCNU), Guangzhou, China.

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http://dx.doi.org/10.1038/s41477-021-00984-0DOI Listing
August 2021

Spherical nucleic acids-based cascade signal amplification for highly sensitive detection of exosomes.

Biosens Bioelectron 2021 Nov 29;191:113465. Epub 2021 Jun 29.

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, PR China; Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai, 200444, PR China. Electronic address:

Exosomes are regarded as a promising biomarker in the diagnosis of disease due to their close relationship with the change of physiology and pathology. However, it is still a hard challenge to come up with a highly sensitive method for the exosomes detection. Herein, we propose a spherical nucleic acids (SNAs)-based cascade signal amplification strategy for the exosomes detection with high sensitivity. In this method, SNAs anchoring on exosomes membrane can be extended to form polyT sequence by terminal deoxynucleotidyl transferase (TdT), generating a template strand for the Exo III-catalyzed excision of the designed signal probe (probe A), which may finally induce significant decrease of electrochemical signal due to the consumption of probe A. Benefiting from the SNAs-based cascade signal amplification, this fabricated biosensor achieves a limit of detection for exosomes as low as 44 particles/μL. Moreover, this method shows good performance in the differentiation of healthy and malignancy colorectal cancer patients. Therefore, without complicated nucleic acids sequences design, our approach provides a cascade signal amplification strategy for the highly sensitive detection of exosomes and shows the potential applications in clinical diagnosis.
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http://dx.doi.org/10.1016/j.bios.2021.113465DOI Listing
November 2021

CircRNA CircRIMS is Overexpressed in Esophageal Squamous Cell Carcinoma and Downregulate miR-613 Through Methylation to Increase Cell Proliferation.

Cancer Manag Res 2021 9;13:4587-4595. Epub 2021 Jun 9.

Department of Gastroenterology, General Hospital of Eastern Theater Command, Nanjing City, Jiangsu Province, 210002, People's Republic of China.

Purpose: CircRNA CircRIMS has been characterized as an oncogenic circRNA in gastric cancer, while its role in other cancers is unknown. This study aimed to explore the role of CircRIMS in esophageal squamous cell carcinoma (ESCC).

Patients And Methods: Tissues collected from 60 ESCC patients were subjected to extractions of total RNA and RT-qPCRs to analyze the differential expression of CircRIMS and miR-613. The 60 ESCC patients were followed up for 5 years to analyze the prognostic value of CircRIMS for ESCC. The interaction between CircRIMS and miR-613 was showed by luciferase activity assay and fluorescence in situ hybridization. The role of CircRIMS in regulating miR-613 expression and methylation was analyzed by overexpression experiments, RT-qPCRs and Western blot assay. The role of CircRIMS and miR-613 in regulating cell proliferation was analyzed using the BrdU assay. ESCC xenograft model was used to demonstrate the role of CircRIMS and miR-613 in vivo.

Results: We found that CircRIMS was overexpressed in ESCC and predicted poor survival. In addition, miR-613 was under expressed in ESCC and inversely correlated with CircRIMS. In ESCC cells, CircRIMS overexpression decreased the expression of miR-613 and increased the methylation of miR-613 gene. Cell proliferation assay showed that CircRIMS overexpression reduced the inhibitory effects of miR-613 overexpression on cell proliferation. Animal experience finally illustrated that CircRNA CircRIMS downregulated miR-613 through methylation to promote tumor growth.

Conclusion: Therefore, CircRIMS may downregulate miR-613 through methylation to increase cell proliferation in ESCC.
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http://dx.doi.org/10.2147/CMAR.S282983DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200154PMC
June 2021

Global Trends in Research of Macrophages Associated With Acute Lung Injury Over Past 10 Years: A Bibliometric Analysis.

Front Immunol 2021 20;12:669539. Epub 2021 May 20.

Department of Anesthesiology, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China.

Acute lung injury (ALI) is an intractable disorder associated with macrophages. This bibliometric analysis was applied to identify the characteristics of global scientific output, the hotspots, and frontiers about macrophages in ALI over the past 10 years. We retrieved publications published from 2011 to 2020 and their recorded information from Science Citation Index Expanded (SCI-expanded) of Web of Science Core Collection (WoSCC). Bibliometrix package was used to analyze bibliometric indicators, and the VOSviewer was used to visualize the trend and hotspots of researches on macrophages in ALI. Altogether, 2,632 original articles were reviewed, and the results showed that the annual number of publications (Np) concerning the role of macrophages in ALI kept increasing over the past 10 years. China produced the most papers, the number of citations (Nc) and H-index of the USA ranked first. Shanghai Jiaotong University and INT IMMUNOPHARMACOL were the most prolific affiliation and journal, respectively. Papers published by Matute-Bello G in 2011 had the highest local citation score (LCS). Recently, the keywords "NLRP3" and "extracellular vesicles" appeared most frequently. Besides, researches on COVID-19-induced ALI related to macrophages seemed to be the hotspot recently. This bibliometric study revealed that publications related to macrophages in ALI tend to increase continuously. China was a big producer and the USA was an influential country in this field. Most studies were mainly centered on basic researches in the past decade, and pathways associated with the regulatory role of macrophages in inhibiting and attenuating ALI have become the focus of attention in more recent studies. What is more, our bibliometric analysis showed that macrophages play an important role in COVID-19-induced ALI and may be a target for the treatment of COVID-19.
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http://dx.doi.org/10.3389/fimmu.2021.669539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173163PMC
June 2021

Role of Ferroptosis in Lung Diseases.

J Inflamm Res 2021 20;14:2079-2090. Epub 2021 May 20.

Department of Anesthesiology, Fuyang Hospital of Anhui Medical University, Fuyang, Anhui, 236000, People's Republic of China.

Ferroptosis is a new type of programmed cell death characterized by intracellular iron accumulation and lipid peroxidation that leads to oxidative stress and cell death. The metabolism of iron, lipids, and amino acids and multiple signalling pathways precisely regulate the process of ferroptosis. Emerging evidence has demonstrated that ferroptosis participates in the occurrence and progression of various pathological conditions and diseases, such as infections, neurodegeneration, tissue ischaemia-reperfusion injury and immune diseases. Recent studies have also indicated that ferroptosis plays a critical role in the pathogenesis of acute lung injury, chronic obstructive pulmonary disease, pulmonary fibrosis, pulmonary infection and asthma. Herein, we summarize the latest knowledge on the regulatory mechanism of ferroptosis and its association with iron, lipid and amino acid metabolism as well as several signalling pathways. Furthermore, we review the contribution of ferroptosis to the pathogenesis of lung diseases and discuss ferroptosis as a novel therapeutic target for various lung diseases.
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http://dx.doi.org/10.2147/JIR.S307081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8144020PMC
May 2021

The clinical effect of emergency gastroscopy on upper gastrointestinal hemorrhage patients.

Am J Transl Res 2021 15;13(4):3501-3507. Epub 2021 Apr 15.

Department of Orthopedics, Guizhou Province Orthopaedic Hospital Guiyang 550007, Guizhou Province, China.

Objective: To assess the clinical effects of emergency gastroscopies on acute upper gastrointestinal hemorrhage patients.

Methods: 212 patients with acute upper gastrointestinal hemorrhages were randomly divided into an experimental group (n=106) and a control group (n=106). The experimental group underwent emergency gastroscopies, and the control group underwent the traditional treatment. We measured the hemostasis effects, the treatment indexes, and the incidences of adverse reactions to assess the clinical effects. At the same time, we recorded the hemoglobin levels, the rebleeding rates, and the mortality rate to assess the hemostasis effect.

Results: The hemostasis effect (the total hemostasis effective rate), the treatment index (the hemostasis time, stool occult blood turning negative time, bowel sound recovery time, and the blood transfusion volumes), the hemoglobin levels, the rebleeding rates, and the mortality were better in the EG than they were in the CG (P<0.05).

Conclusions: Emergency gastroscopy is an effective treatment for acute upper gastrointestinal hemorrhage patients, because it improves the hemostasis effective rate and the survival rate. Clinical therapy effectively cures the hemorrhages in patients with acute upper gastrointestinal hemorrhages.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129367PMC
April 2021

Highly efficient generation of bacterial leaf blight-resistant and transgene-free rice using a genome editing and multiplexed selection system.

BMC Plant Biol 2021 Apr 24;21(1):197. Epub 2021 Apr 24.

Syngenta Biotechnology (China) Co., Ltd, No.25, Life Science Park Road, Beijing, 102206, China.

Background: Rice leaf blight, which is a devastating disease worldwide, is caused by the bacterium Xanthomonas oryzae pv. oryzae (Xoo). The upregulated by transcription activator-like 1 (UPT) effector box in the promoter region of the rice Xa13 gene plays a key role in Xoo pathogenicity. Mutation of a key bacterial protein-binding site in the UPT box of Xa13 to abolish PXO99-induced Xa13 expression is a way to improve rice resistance to bacteria. Highly efficient generation and selection of transgene-free edited plants are helpful to shorten and simplify the gene editing-based breeding process. Selective elimination of transgenic pollen of T0 plants can enrich the proportion of T1 transgene-free offspring, and expression of a color marker gene in seeds makes the selection of T2 plants very convenient and efficient. In this study, a genome editing and multiplexed selection system was used to generate bacterial leaf blight-resistant and transgene-free rice plants.

Results: We introduced site-specific mutations into the UPT box using CRISPR/Cas12a technology to hamper with transcription-activator-like effector (TAL) protein binding and gene activation and generated genome-edited rice with improved bacterial blight resistance. Transgenic pollen of T0 plants was eliminated by pollen-specific expression of the α-amylase gene Zmaa1, and the proportion of transgene-free plants increased from 25 to 50% among single T-DNA insertion events in the T1 generation. Transgenic seeds were visually identified and discarded by specific aleuronic expression of DsRed, which reduced the cost by 50% and led to up to 98.64% accuracy for the selection of transgene-free edited plants.

Conclusion: We demonstrated that core nucleotide deletion in the UPT box of the Xa13 promoter conferred resistance to rice blight, and selection of transgene-free plants was boosted by introducing multiplexed selection. The combination of genome editing and transgene-free selection is an efficient strategy to accelerate functional genomic research and plant breeding.
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http://dx.doi.org/10.1186/s12870-021-02979-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066475PMC
April 2021

Molecular Disorder Induces an Unusual Phase Transition in a Potential 2D Chiral Ferroelectric Perovskite.

Chemistry 2021 Jun 19;27(35):9054-9059. Epub 2021 May 19.

Hubei Key Laboratory of Optical Information and Pattern Recognition, Wuhan Institute of Technology, Wuhan, 430205, P. R. China.

Two-dimensional hybrid halide perovskites with single chiral and ferroelectricity together with various structural phase transitions provide the possibility for more diverse functional properties. Here, we present a 2D chiral hybrid halide perovskite ferroelectric, [C H (CH ) NH ] CdCl (4PBA-CdCl , 4PBA=4-phenylbutylamine) that experiences two continuous phase transitions from centrosymmetric triclinic P to polar chiral monoclinic P2 and then to another centrosymmetric tetragonal P4/mmm with increasing temperature, accompanied by symmetry breaking, due to the prominent octahedral distortion and disorder transformation of organic 4PBA cations. In the polar chiral phase, 4PBA-CdCl gives a significant CD signal and has a moderate ferroelectric polarization of 0.35 μC/cm . In addition, 4PBA-CdCl occupies a wide band gap of 4.376 eV that is chiefly contributed by the inorganic CdCl octahedron. This finding offers an alternative pathway for designing new phase transitions and related physical properties in hybrid halide perovskites and other hybrid crystals.
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http://dx.doi.org/10.1002/chem.202100334DOI Listing
June 2021

An investigation on gastric cancer staging using CT structured report.

Eur J Radiol 2021 Mar 13;136:109550. Epub 2021 Jan 13.

Department of Radiology, Peking University First Hospital, Beijing, China. Electronic address:

Objective: To evaluate the clinical performance of structured report (SR) for CT in patients with pre-operative staging of gastric cancer, compared to non-SR.

Methods: Retrospectively, 51 consecutive cases with primary gastric cancer staging were enrolled. Every SR or non-SR was performed by two GI radiologists (1 junior and 1 senior). Interobserver agreement was conducted between the junior and senior groups for both SR and non-SR. 10 key features required for lesion description and staging were assessed between SR and non-SR. Diagnostic content between SR and non-SR was also compared. Accuracy of SR and non-SR on T staging was measured. Subjective evaluation of SR vs. non-SR was also conducted in form of survey by 20 radiologists and 3 GI surgeons.

Results: Interobserver agreement showed excellent in SR (Kappa = 1, P < 0.001), but poor in non-SR (Kappa = 0.036, P = 0.455). For the 10 key features required for lesion assessment, non-SR showed 6.84 ± 0.83 while SR reported all of them (P < 0.001). Statistically significant improvement was observed in the SR for parts of key features, especially for assessment of adjacent organs and vessels (P < 0.001). Accuracy comparison of T staging showed higher in SR for cohort of T4a (P = 0.028<0.05). The scores of subjective evaluation were higher (P < 0.05) in SR than in non-SR by both radiologists and surgeons. Meanwhile, the inter-observer agreement among surgeons was good in SR with significance (w=0.53, P = 0.005 for efficiency; w=0.638, P < 0.001 for integrity) but poor in non-SR.

Conclusions: SR of gastric multiphasic CT ensured reliable detection of all relevant key features for staging along with reproducible documentation, which was not always the case for non-SR. In addition, SR has the potential in improving diagnostical accuracy of T staging and was welcomed by clinicians.
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http://dx.doi.org/10.1016/j.ejrad.2021.109550DOI Listing
March 2021

Generation of paternal haploids in wheat by genome editing of the centromeric histone CENH3.

Nat Biotechnol 2020 12 9;38(12):1397-1401. Epub 2020 Nov 9.

Seeds Research, Syngenta Crop Protection, LLC, Research Triangle Park, NC, USA.

New breeding technologies accelerate germplasm improvement and reduce the cost of goods in seed production. Many such technologies could use in vivo paternal haploid induction (HI), which occurs when double fertilization precedes maternal (egg cell) genome loss. Engineering of the essential CENTROMERIC HISTONE (CENH3) gene induces paternal HI in Arabidopsis. Despite conservation of CENH3 function across crops, CENH3-based HI has not been successful outside of the Arabidopsis model system. Here we report a commercially operable paternal HI line in wheat with a ~7% HI rate, identified by screening genome-edited TaCENH3α-heteroallelic combinations. Unlike in Arabidopsis, edited alleles exhibited reduced transmission in female gametophytes, and heterozygous genotypes triggered higher HI rates than homozygous combinations. These developments might pave the way for the deployment of CENH3 HI technology in diverse crops.
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http://dx.doi.org/10.1038/s41587-020-0728-4DOI Listing
December 2020

Ultrasensitive label-free immunochromatographic strip sensor for Salmonella determination based on salt-induced aggregated gold nanoparticles.

Food Chem 2021 May 31;343:128518. Epub 2020 Oct 31.

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, PR China. Electronic address:

Here we present an innovative label-free immunochromatographic strip (ICTS) sensor, in which salt-induced aggregated gold nanoparticles (SIA-AuNPs) act as the signal probe, allowing in 14 min the identification and sensitive quantification of Salmonella as model targets. It has been evidenced that SIA-AuNPs could be absorbed on the surface of bacteria based on van der Waals forces. The [email protected] complex was captured by anti-Salmonella polyclonal antibody deposited on the test zone. With the label-free ICTS sensor, we successfully detected Salmonella in a concentration range of 10-10 CFU/mL and a visual detection limit of 1 × 10 CFU/mL. The band of test zone could be distinguished at a concentration of 10 CFU/mL by naked eye, which is 100-fold lower than the cationic AuNPs based method. The strip sensor was further validated with real samples including cabbage and drinking water with excellent precision and showed to provide excellent recovery.
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http://dx.doi.org/10.1016/j.foodchem.2020.128518DOI Listing
May 2021

High-throughput sequencing provides insights into oral microbiota dysbiosis in association with inflammatory bowel disease.

Genomics 2021 Jan 1;113(1 Pt 2):664-676. Epub 2020 Oct 1.

Department of Gastroenterology and Hepatology, Jinling Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China.. Electronic address:

Although the prevalence of inflammatory bowel disease (IBD) has been increasing worldwide, the etiology remains elusive. Investigating oral microbiota dysbiosis is essential to understanding IBD pathogenesis. Our study evaluated variations in salivary microbiota and identified potential associations with IBD. The saliva microbiota of 22 IBD patients and 8 healthy controls (HCs) was determined using 16S ribosomal RNA (rRNA) gene sequencing and analyzed using QIIME2. A distinct saliva microbiota dysbiosis in IBD, characterized by alterations in microbiota biodiversity and composition, was identified. Saccharibacteria (TM7), Absconditabacteria (SR1), Leptotrichia, Prevotella, Bulleidia, and Atopobium, some of which are oral biofilm-forming bacteria, were significantly increased. Moreover, levels of inflammatory cytokines associated with IBD were elevated and positively correlated with TM7 and SR1. Functional variations include down-regulation of genetic information processing, while up-regulation of carbohydrate metabolism and protein processing in the endoplasmic reticulum in IBD. Our data implicate salivary microbiota dysbiosis involving in IBD pathogenesis.
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http://dx.doi.org/10.1016/j.ygeno.2020.09.063DOI Listing
January 2021

Accurate Identification of Isomeric Glycans by Trapped Ion Mobility Spectrometry-Electronic Excitation Dissociation Tandem Mass Spectrometry.

Anal Chem 2020 10 11;92(19):13211-13220. Epub 2020 Sep 11.

Center for Biomedical Mass Spectrometry, Boston University School of Medicine, 670 Albany Street, Room 508, Boston, Massachusetts 02118, United States.

Ion mobility-mass spectrometry (IM-MS) has become a powerful tool for glycan structural characterization due to its ability to separate isomers and provide collision cross section (CCS) values that facilitate structural assignment. However, IM-based isomer analysis may be complicated by the presence of multiple gas-phase conformations of a single structure that not only increases difficulty in isomer separation but can also introduce the possibility for misinterpretation of conformers as isomers. Here, the ion mobility behavior of several sets of isomeric glycans, analyzed as their permethylated derivatives, in both nonreduced and reduced forms, was investigated by gated-trapped ion mobility spectrometry (G-TIMS). Notably, reducing-end reduction, commonly performed to remove anomerism-induced chromatographic peak splitting, did not eliminate the conformational heterogeneity of permethylated glycans in the gas phase. At a mobility resolving power of ∼100, 14 out of 22 structures showed more than one conformation. These results highlight the need to use IMS devices with high mobility resolving power for better separation of isomers and to acquire additional structural information that can differentiate isomers from conformers. Online electronic excitation dissociation (EED) MS/MS analysis of isomeric glycan mixtures following G-TIMS separation showed that EED can generate isomer-specific fragments while producing nearly identical tandem mass spectra for conformers, thus allowing confident identification of isomers with minimal evidence of any ambiguity resulting from the presence of conformers. G-TIMS EED MS/MS analysis of -linked glycans released from ovalbumin revealed that several mobility features previously thought to arise from isomeric structures were conformers of a single structure. Finally, analysis of ovalbumin -glycans from different sources showed that the G-TIMS EED MS/MS approach can accurately determine the batch-to-batch variations in glycosylation profiles at the isomer level, with confident assignment of each isomeric structure.
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http://dx.doi.org/10.1021/acs.analchem.0c02374DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111820PMC
October 2020

Electroacupuncture Pretreatment Alleviates LPS-Induced Acute Respiratory Distress Syndrome via Regulating the PPAR Gamma/NF-Kappa B Signaling Pathway.

Evid Based Complement Alternat Med 2020 22;2020:4594631. Epub 2020 Jul 22.

Department of Anesthesiology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai 200433, China.

Electroacupuncture (EA) is reported to possess anti-inflammatory properties and has beneficial effects on acute respiratory distress syndrome (ARDS). However, the underlying mechanisms of the effects of EA on ARDS remain unclear. This study aims to investigate the protective effect of EA on LPS-induced ARDS. In this study, Sprague-Dawley male rats were treated with EA at Hegu (LI4) for 45 minutes before LPS instillation (0.4 mg/kg, 100 ul). H&E staining, wet-to-dry weight (W/D) ratio, PaO, and protein content in BALF were employed to determine the function of lung tissues. Inflammatory cytokines in serum and BALF were detected by enzyme-linked immunoassay assay (ELISA). The levels of oxidative stress markers were detected to determine the oxidative stress status. Cell apoptosis was observed by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining and western blot. Here, we found that EA pretreatment effectively alleviated lung pathological damage. Moreover, EA suppressed the oxidative stress damage by upregulating glutathione and superoxide dismutase and downregulating malondialdehyde. EA pretreatment also regulated apoptosis-related proteins, such as Bax and Bcl-2. We found that peroxisome proliferators-activated receptors (PPAR) play a critical role during ARDS, EA up-regulated the expression of PPAR, which inhibited the activation of nuclear factor-kappa B (NF-B) and decreased the inflammatory cytokines (interleukin-1, interleukin-6, and tumor necrosis factor-). When rats were treated with GW9662, a selective PPAR antagonist, these effects of EA were reversed. Our study demonstrated that EA pretreatment had a beneficial effect on LPS-induced ARDS in rats by anti-inflammatory, antioxidative, and antiapoptotic properties which was regulated via PPAR/NF-B signaling pathway.
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http://dx.doi.org/10.1155/2020/4594631DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396021PMC
July 2020

Functional Analysis of Plant FYVE Domain Proteins in Endosomal Trafficking.

Methods Mol Biol 2020 ;2177:83-94

Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, China.

The FYVE domain is a double zinc finger-like domain that predominantly binds phosphatidylinositol 3-phosphate. The FYVE domain is usually found in proteins primarily involved in regulating various aspects of endomembrane homeostasis, including endosome tethering, endocytic recycling, membrane protein sorting, and autophagosome maturation. Whereas FYVE domain proteins have been extensively studied in mammals and yeast, only a few FYVE domain proteins have been identified and characterized in plants. Here, by using as an example FREE1 (FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING 1), a protein previously identified by us as a critical factor for endosomal trafficking, we describe methods to determine its lipid binding properties and endosomal localization. In addition, we also demonstrate a method to quickly test whether an FYVE domain protein is involved in endosomal sorting in plant cells.
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http://dx.doi.org/10.1007/978-1-0716-0767-1_8DOI Listing
March 2021

Air-Stable Organic Radicals: New-Generation Materials for Flexible Electronics?

Adv Mater 2020 Aug 25;32(32):e1908015. Epub 2020 Jun 25.

Frontiers Science Center for Flexible Electronics (FSCFE), Shaanxi Institute of Flexible Electronics (SIFE) & Shaanxi Institute of Biomedical Materials and Engineering (SIBME), Northwestern Polytechnical University, Xi'an, Shaanxi, 710072, China.

In the last few years, air-stable organic radicals and radical polymers have attracted tremendous attention due to their outstanding performance in flexible electronic devices, including transistors, batteries, light-emitting diodes, thermoelectric and photothermal conversion devices, and among many others. The main issue of radicals from laboratory studies to real-world applications is that the number of known air-stable radicals is very limited, and the radicals that have been used as materials are even less. Here, the known and newly developed air-stable organic radicals are summarized, generalizing the way of observing air-stable radicals. The special electric and photophysical properties of organic radicals and radical polymers are interpreted, which give radicals a wide scope for various of potential applications. Finally, the exciting applications of radicals that have been achieved in flexible electronic devices are summarized. The aim herein is to highlight the recent achievements in radicals in chemistry, materials science, and flexible electronics, and further bridge the gap between these three disciplines.
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http://dx.doi.org/10.1002/adma.201908015DOI Listing
August 2020

Bone Marrow Mesenchymal Stem Cell-Derived Exosomes Attenuate LPS-Induced ARDS by Modulating Macrophage Polarization Through Inhibiting Glycolysis in Macrophages.

Shock 2020 12;54(6):828-843

Department of Anesthesiology, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.

Macrophages play a key role in the development of sepsis-induced acute respiratory distress syndrome (ARDS). Recent evidence has proved that glycolysis plays an important role in regulating macrophage polarization through metabolic reprogramming. Bone marrow mesenchymal stem cells (BMSCs) can alleviate sepsis-induced lung injury and possess potent immunomodulatory and immunosuppressive properties via secreting exosomes. However, it is unknown whether BMSCs-derived exosomes exert their therapeutic effect against sepsis-induced lung injury by inhibiting glycolysis in macrophages. Therefore, the present study aimed to evaluate the anti-inflammatory effects of exosomes released from BMSCs on acute lung injury induced by lipopolysaccharide (LPS) in mice and explored the possible underlying mechanisms in vitro and in vivo. We found that BMSCs inhibited M1 polarization and promoted M2 polarization in MH-S cells (a murine alveolar macrophage cell line) by releasing exosomes. Further experiments showed that exosomes secreted by BMSCs modulated LPS-treated MH-S cells polarization by inhibiting cellular glycolysis. Moreover, our results showed that BMSCs-derived exosomes down-regulated the expression of several essential proteins of glycolysis via inhibition of hypoxia-inducible factor 1 (HIF-1)α. Finally, a model of LPS-induced ARDS in mice was established, we found that BMSCs-derived exosomes ameliorated the LPS-induced inflammation and lung pathological damage. Meanwhile, we found that intratracheal delivery of BMSCs-derived exosomes effectively down-regulated LPS-induced glycolysis in mice lung tissue. These findings reveal new mechanisms of BMSCs-derived exosomes in regulating macrophage polarization which may provide novel strategies for the prevention and treatment of LPS-induced ARDS.
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http://dx.doi.org/10.1097/SHK.0000000000001549DOI Listing
December 2020

Hsa-miR-217 Inhibits the Proliferation, Migration, and Invasion in Non-small Cell Lung Cancer Cells Via Targeting SIRT1 and P53/KAI1 Signaling

Balkan Med J 2020 06 9;37(1):208-214. Epub 2020 Apr 9.

Tongji University School of Medicine, Shanghai, China

Background: Brain metastasis is a major cause of cancer death in patients with lung cancer. Sirtuin 1 and hsa-miR-217 have been identified to mediate the development of non-small cell lung cancer.

Aims: To investigate the roles of hsa-miR-217, its target sirtuin 1, and the P53/KAI1 axis in the brain metastasis from non-small cell lung cancer.

Study Design: Cell culture study.

Methods: Human pulmonary adenocarcinoma brain metastasis cell line PC-14/B were incubated and treated with constructed lentiviral plasmids expressing miR-217 and/or sirtuin 1. BEAS-2B cell line was used as a control. The targeted regulation of miR-217 to sirtuin 1was examined using a dual-luciferase reporter assay. Cell proliferation, migration, invasion, and related protein expression were detected to examine the effect of the miR-217/sirtuin 1 expression on metastasis.

Results: PC-14/B cells expressed higher sirtuin 1 and lower P53 and KAI1 compared with BEAS-2B control cells (p<0.05). Sirtuin 1 was a direct target of miR-217. MiR-217 expression suppressed PC-14/B cell invasion (p=0.004), migration (p=0.001), and proliferation (p<0.05), whereas sirtuin 1 overexpression reversed all processes. sirtuin 1 expression inhibited P53, KAI1/CD82, matrix metalloproteinase-9, and β-catenin but upregulated E-cadherin protein. MiR-217 overexpression induced reverse changes.

Conclusion: Hsa-miR-217 and its target sirtuin 1 acted as metastasis suppressor and promoter gene in non-small cell lung cancer, respectively. The hsa-miR-217/sirtuin 1/P53/KAI1 metastasis regulatory pathway showed novel and crucial roles in brain metastasis from non-small cell lung cancer. This axis might be a potential target for the treatment of brain metastasis of lung cancer.
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http://dx.doi.org/10.4274/balkanmedj.galenos.2020.2019.9.91DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7285661PMC
June 2020

Cisplatin resistance-associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma.

Exp Ther Med 2020 Apr 12;19(4):2733-2740. Epub 2020 Feb 12.

Department of Gastroenterology and Hepatology, Huainan First People's Hospital and First Affiliated Hospital of The Medical College of Anhui University of Science and Technology, Huainan, Anhui 232000, P.R. China.

Hepatocellular carcinoma (HCC) is a leading cause of cancer-associated mortality worldwide. Despite clinical advances, the survival rate of patients with HCC remains low, as most patients are diagnosed with HCC when they are already at the advanced stage. Certain circular RNAs (circRNAs) are closely associated with the development of liver cancer. In the present study, a circRNA array was performed to screen differentially expressed circRNAs in HCC tissues. The further analysis focused on the newly identified circRNA_101237, the host gene of which, cyclin-dependent kinase 8, is located at chr13:26974589-26975761. CircRNA_101237 was determined to be upregulated in tumor tissue and serum of patients with HCC as compared with that in paracancerous tissues and the serum of healthy controls, respectively. In addition, the expression of circRNA_101237 was associated with tumor size, lymph node metastasis, distant metastasis and TNM stage. Univariate and multivariate analysis indicated that serum circRNA_101237 levels were an independent predictor of survival prognosis in patients with HCC. The overall survival of patients with high expression of circRNA_101237 was reduced compared with that of patients with low expression of circRNA_101237. Of note, cisplatin induced the expression of circRNA_101237 in HCC cells in a dose- and time-dependent manner , and the levels of circRNA_101237 in the serum of patients with cisplatin-resistant HCC and in cisplatin-resistant Huh7 cells were increased. The present study provided novel insight into the use of circRNA_101237 as a diagnostic biomarker for HCC and a potential therapeutic target.
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http://dx.doi.org/10.3892/etm.2020.8526DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092923PMC
April 2020

circHIPK3 Promotes Cell Proliferation and Migration of Gastric Cancer by Sponging miR-107 and Regulating BDNF Expression.

Onco Targets Ther 2020 21;13:1613-1624. Epub 2020 Feb 21.

Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, People's Republic of China.

Background: Circular RNAs (circRNAs) play important regulatory roles in cancer development. However, the mechanisms by which circRNAs regulate gene expression in gastric cancer (GC) remain unclear.

Methods: Human GC samples and their matched normal adjacent tissues were obtained from 30 patients to assess the expression of circHIPK3 and its relationship with GC proliferation and migration. A series of in vitro and in vivo functional experiments were carried out to elucidate the role of circHIPK3 in GC proliferation and migration, and its underlying molecular mechanisms.

Results: Using a circRNA microarray we found a circRNA termed circHIPK3 that performed a significant regulatory role in GC. circHIPK3 was further confirmed to be upregulated in all GC tissues and cells tested. Furthermore, circHIPK3 levels were associated with Tumor & Lymph Node & Metastasis(TNM) stage (P = 0.032). The area under the receiver operating characteristic curve (ROC) was 0.743 (95% confidence interval 0.615-0.872; P = 0.001). CCK-8, colony formation, Transwell and EdU assays were performed to evaluate the effects of circHIPK3 on cell proliferation and migration in GC. Moreover, circHIPK3 was identified as a sponge of miR-107, and as such it regulated brain-derived neurotrophic factor (BDNF), which plays a pivotal role in the development of GC.

Conclusion: circHIPK3 represents a novel potential biomarker and therapeutic target of GC.
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http://dx.doi.org/10.2147/OTT.S226300DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041611PMC
February 2020

HY5-HDA9 Module Transcriptionally Regulates Plant Autophagy in Response to Light-to-Dark Conversion and Nitrogen Starvation.

Mol Plant 2020 03 19;13(3):515-531. Epub 2020 Feb 19.

Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China. Electronic address:

Light is arguably one of the most important environmental factors that determines virtually all aspects of plant growth and development, but the molecular link between light signaling and the autophagy pathway has not been elucidated in plants. In this study, we demonstrate that autophagy is activated during light-to-dark conversion though transcriptional upregulation of autophagy-related genes (ATGs). We showed that depletion of the ELONGATED HYPOCOTYL 5 (HY5), a key component of light signaling, leads to enhanced autophagy activity and resistance to extended darkness and nitrogen starvation treatments, contributing to higher expression of ATGs. HY5 interacts with and recruits HISTONE DEACETYLASE 9 (HDA9) to ATG5 and ATG8e loci to repress their expression by deacetylation of the Lys9 and Lys27 of histone 3. Furthermore, we found that both darkness and nitrogen depletion induce the degradation of HY5 via 26S proteasome and the concomitant disassociation of HDA9 from ATG5 and ATG8e loci, leading to their depression and thereby activated autophagy. Genetic analysis further confirmed that HY5 and HDA9 act synergistically and function upstream of the autophagy pathway. Collectively, our study unveils a previously unknown transcriptional and epigenetic network that regulates autophagy in response to light-to-dark conversion and nitrogen starvation in plants.
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http://dx.doi.org/10.1016/j.molp.2020.02.011DOI Listing
March 2020

Use of a submandibular gland flap for closure of oral cutaneous fistula.

Oral Oncol 2020 May 31;104:104583. Epub 2020 Jan 31.

Department of Oral and Maxillofacial Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China. Electronic address:

We reported a rare case of submandibular oral cutaneous fistula (OCF) in a 27-year-old female. A submandibular gland flap (SMGF) was prepared after fistula resection to fill the dead space under the mouth floor. Based on this case report and a literature review, we discussed the pathogenesis and treatment of OCF, and compared the differences between the SMGF and other types of flaps.
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http://dx.doi.org/10.1016/j.oraloncology.2020.104583DOI Listing
May 2020

Selective and Colorimetric Detection of p-Nitrophenol Based on Inverse Opal Polymeric Photonic Crystals.

Polymers (Basel) 2020 Jan 3;12(1). Epub 2020 Jan 3.

Key Laboratory of Auxiliary Chemistry and Technology for Chemical Industry, Ministry of Education, Shaanxi University of Science and Technology, Xi'an 710021, China.

The detection of p-nitrophenol (PNP) is of great significance for assessment of environment pollution and potential health risks. In this study, based on inverse opal polymeric photonic crystals (IOPPCs), a selective and visual sensor for high-performance PNP detection is developed. Due to their unique optical properties, IOPPCs report events by change of color, which can easily be observed by the naked eye. Hydroxyethyl methacrylate (HEMA) was selected as the functional monomer with which to fabricate the IOPPCs. By precisely adjusting the molar ratio between the functional monomer and the crosslinker, the sensors were only able to be sensitive to a specific solution, thus realizing the visual, selective, and semi-quantitative detection of PNP. When the sensors were immersed in different concentrations of PNP solution, their Bragg diffraction wavelengths showed different redshifts. The color of the IOPPCs changed from green to red as the peak shift of Bragg diffraction occurred. In addition, the IOPPCs displayed good interference immunity and reusability.
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http://dx.doi.org/10.3390/polym12010083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023634PMC
January 2020

A Comparative Study of Two Tube Feeding Methods in Patients with Dysphagia After Stroke: A Randomized Controlled Trial.

J Stroke Cerebrovasc Dis 2020 Mar 8;29(3):104602. Epub 2020 Jan 8.

Department of Rehabilitation Medicine, Guangzhou Panyu Central Hospital, China.

Background: Dysphagia is a common symptom seen in stroke patients, it not only affects patients' nutrition supply, but also causes aspiration pneumonia. To solve the problem of nutritional support for patients with dysphagia after stroke, nasogastric tubes are routinely indwelling to provide nutrition in China. However, this feeding method sometimes causes food reflux, aspiration, pneumonia, and often affects the patients' comfort and self-image acceptance.

Aim: The aim of this study was to determine whether a new feeding method called intermittent oroesophageal (IOE) tube feeding compared with continuous nasogastric tube feeding as a practical and beneficial mean of decreasing the rate of stroke associated pneumonia (SAP), and improving patients' swallowing function, comfort, psychological status.

Design: This was an assessor-blinded, single-center, randomized controlled trial.

Methods: Ninety-seven hospitalized stroke patients with dysphagia in the rehabilitation department from January to December 2018 were randomized to a control group and an intervention group. Patients in both groups received routine nursing, rehabilitation treatment and swallowing therapy. Patients in the intervention group were given IOE tube feeding, while those in the control group were fed by indwelling nasogastric tube. Outcomes were assessed at admission, discharge or the end of the tube feeding.

Results: The incidence of SAP in the intervention group was 16.33% lower than that (31.25%) in the control group; the comfort score (2.08 ± .64), anxiety score (10.98 ± 2.28), depression score (7.39 ± 2.16) were lower than those (3.02 ± .70), (12.10 ± 2.18), (8.42 ± 2.34) in the control group. The improvement rate of swallowing function in the intervention group was 83.67% higher than that (66.67%) in the control group (all P < .05).

Conclusions: The IOE tube feeding compared with continuous tube feeding may reduce the incidence of SAP, and improve patients' swallowing function, comfort, psychological status in patients with dysphagia after stroke.
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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2019.104602DOI Listing
March 2020

Determination of the Aggregate Binding Site of Amyloid Protofibrils Using Electron Capture Dissociation Tandem Mass Spectrometry.

J Am Soc Mass Spectrom 2020 Feb 10;31(2):267-276. Epub 2020 Jan 10.

Department of Chemistry, Gibbet Hill Road , University of Warwick , Coventry CV4 7AL , United Kingdom.

Amyloid fibril formation is a hallmark in a range of human diseases. Analysis of the molecular details of amyloid aggregation, however, is limited by the difficulties in solubilizing, separating, and identifying the aggregated biomolecules. Additional labeling or protein modification is required in many current analytical techniques in order to provide molecular details of amyloid protein aggregation, but these modifications may result in protein structure disruption. Herein, ultrahigh resolution mass spectrometry (MS) with electron capture dissociation tandem MS (ECD MS/MS) has been applied to monitor the formation of early oligomers of human islet amyloid polypeptide (hIAPP), which aggregate rapidly in the pancreas of type II diabetes (T2D) patients. ECD MS/MS results show the aggregation region of the early oligomers is at the Ser-28/Ser-29 residue of a hIAPP unit and at the Asn-35 residue of another hIAPP unit near the C-terminus in the gas phase. These data contribute to the understanding of the binding site between hIAPP units which may help for specific target region therapeutic development in the future. Furthermore, MS has also been applied to quantify the amount of soluble amyloid protein remaining in the incubated solutions, which can be used to estimate the aggregation rate of amyloid protein during incubation (28 days). These data are further correlated with the results obtained using fluorescence spectroscopy and transmission electron microscopy (TEM) to generate a general overview of amyloid protein aggregation. The methods demonstrated in this article not only explore the aggregation site of hIAPP down to an amino acid residue level, but are also applicable to many amyloid protein aggregation studies.
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http://dx.doi.org/10.1021/jasms.9b00053DOI Listing
February 2020
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