Publications by authors named "Juan Peng"

299 Publications

Early prediction of response to neoadjuvant chemotherapy using contrast-enhanced ultrasound in breast cancer.

Medicine (Baltimore) 2021 May;100(19):e25908

Department of Medical Ultrasound.

Abstract: Early prediction of non-response is essential in order to avoid inefficient treatments. The objective of this study was to determine the contrast-enhanced ultrasound (CEUS) for early predicting pathologic complete response (pCR) to neoadjuvant chemotherapy (NAC) in breast cancer patients.Between March 2018 and October 2019, 93 consecutive patients with histologically proven breast cancer scheduled for NAC were enrolled. Conventional ultrasound and CEUS imaging were performed before NAC and after two cycles of NAC. CEUS parameters were compared with pathologic response. Multiple logistic regression analyses were utilized to explore CEUS parameters to predict pCR, and receiver operating characteristic analysis was used to evaluate the predictive ability.Therapeutic response was obtained from 25 (27%) patients with pCR and 68 (73%) with non-pCR. Compared to non-pCR, pCR cases have a significantly higher proportion of homogeneous enhancement feature (56% vs 14%, P < .001) and centripetal enhancement (52% vs 23%, P = .012). A significant decrease in peak intensity (PI) was observed after two cycles of NAC. Compared with non-pCR patients, the kinetic parameters PI change (PI%) was higher in pCR patients (P < .001). Multiple logistic regression demonstrated two independent predictors of pCR: internal homogeneity (odds ratio, 4.85; 95% confidence interval: 1.20-19.65; P = .027) and PI% (odds ratio, 1.08; 95% confidence interval: 1.02-1.15; P = .007). In receiver operating characteristic curve analysis, internal homogeneity and PI%, with area under curve of 0.71 and 0.84, predicted pCR with sensitivity (56%, 95%) and specificity (85%, 70%), respectively.Internal homogeneity and PI% of CEUS may be useful in the noninvasive early prediction of pCR in patients with breast cancer.
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http://dx.doi.org/10.1097/MD.0000000000025908DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133101PMC
May 2021

Distress Trajectories in Black and White Breast Cancer Survivors: From Diagnosis to Survivorship.

Psychoneuroendocrinology 2021 May 27;131:105288. Epub 2021 May 27.

Institute for Behavioral Medicine Research, The Ohio State University College of Medicine, USA; Department of Psychiatry and Behavioral Health, The Ohio State University College of Medicine, USA.

Background: Black breast cancer survivors have greater morbidity and mortality than White survivors. However, evidence comparing Black survivors' psychological symptoms with their White counterparts has been mixed. Prior studies have not compared Black and White survivor's distress-related symptom trajectories from pre- to post-treatment - the goal of the current study.

Methods: At three annual visits from shortly after diagnosis to 6 and 18 months post-treatment, 195 women (n = 163 White; n = 32 Black) reported their cancer-related distress (intrusive thoughts and avoidance), perceived stress, anxiety and depressive symptoms, fatigue, and pain.

Results: Adjusting for age, educational attainment, income, treatment type, stage at diagnosis, and physical comorbidities, Black and White breast cancer survivors had different trajectories of cancer-related distress (p = .004), intrusive thoughts about cancer diagnosis and treatment (p = .002), perceived stress (p = .04), emotional fatigue (p = .01), and vigor (p = .02). Specifically, among White women, these distress-related symptoms improved from diagnosis to 6 months post-treatment (ps < 0.0001) and then remained stable between 6 and 18 months post-treatment, whereas Black women had persistently elevated distress - even 18 months after finishing treatment. Additionally, Black women reported more avoidance of cancer-related thoughts and emotions across visits (p = .047). Race was unrelated to the trajectories of anxiety and depressive symptoms, other fatigue subscales, or pain levels (ps > 0.08).

Conclusion: Longitudinal assessment of the same breast cancer survivors from diagnosis to early survivorship revealed that Black and White survivors had divergent trajectories of psychological distress symptoms that were not reliably evident at a single timepoint. Overall, White women reported less psychological distress from pre- to post-treatment, but Black women's distress remained high from diagnosis to 18 months post-treatment. If left untreated, Black women's high distress levels may contribute to their poorer health throughout survivorship.
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http://dx.doi.org/10.1016/j.psyneuen.2021.105288DOI Listing
May 2021

Effect of PCSK9 on Vascular Smooth Muscle Cell Functions: A New Player in Atherosclerosis.

Curr Med Chem 2021 May 31. Epub 2021 May 31.

Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan Province, Hunan International Scientific and Technological Cooperation Base of Arteriosclerotic Disease, Hengyang Medical College, University of South China, Hengyang City, Hunan Province 421001, China.

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a secretory serine protease that plays multiple biological functions in the regulation of physiological and pathological processes. PCSK9 inhibitors decrease the circulating LDL-cholesterol level with well-known preventive and therapeutic effects on atherosclerosis (AS), but increasing evidence shows that the direct impact of PCSK9 on the vascular wall also plays an important role in atherosclerotic progression. Compared with other vascular cells, a large proportion of PCSK9 is originated from vascular smooth muscle cells (VSMC). Therefore, defining the effect of VSMC-derived PCSK9 on response changes, such as phenotypic switch, apoptosis, autophagy, inflammation, foam cell formation, and calcification of VSMC, helps us better understand the "pleiotropic" effects of VSMC on the atherosclerotic process. In addition, our understanding of the mechanisms of PCSK9 controlling VSMC functions in vivo is far from enough. This review aims to holistically evaluate and analyze the current state of our knowledge regarding PCSK9 actions affecting on VSMC functions and its mechanism in atherosclerotic lesion development. A mechanistic understanding of PCSK9 effects on VSMC will further underpin the success of a new therapeutic strategy targeting AS.
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http://dx.doi.org/10.2174/0929867328666210531150302DOI Listing
May 2021

A study of the correlations between IVIM-DWI parameters and the histologic differentiation of hepatocellular carcinoma.

Sci Rep 2021 May 17;11(1):10392. Epub 2021 May 17.

Medical Imaging Key Laboratory of Sichuan Province, Medical Research Center, Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000, Sichuan, People's Republic of China.

The present study aimed to investigate the value of intravoxel incoherent motion diffusion weighted imaging (IVIM-DWI) in the preoperative prediction of the histologic differentiation of hepatocellular carcinoma (HCC). Seventy HCC patients were scanned with a 3.0 T magnetic resonance scanner. The values of apparent diffusion coefficient (ADC), slow apparent diffusion coefficient (D), fast apparent diffusion coefficient (D*), and the fraction of the fast apparent diffusion coefficient (f) were measured. Analysis of variance was used to compare the differences in parameters between groups with different degrees of histologic differentiation. p < 0.05 was considered statistically significant. Receiver operating characteristic (ROC) curves were used to analyse the efficacy of IVIM-DWI parameters for predicting the histologic differentiation of HCC. The ADC and D values for well, moderately and poorly differentiated HCC were 1.35 ± 0.17 × 10 mm/s, 1.16 ± 0.17 × 10 mm/s, 0.98 ± 0.21 × 10 mm/s, and 1.06 ± 0.15 × 10 mm/s, 0.88 ± 0.16 × 10 mm/s, 0.76 ± 0.18 × 10 mm/s, respectively, and all differences were significant. The D* and f values of the three groups were 32.87 ± 14.70 × 10 mm/s, 41.68 ± 17.90 × 10 mm/s, 34.54 ± 18.60 × 10 mm/s and 0.22 ± 0.07, 0.23 ± 0.08, 0.18 ± 0.07, respectively, with no significant difference. When the cut-off values of ADC and D were 1.25 × 10 mm/s and 0.97 × 10 mm/s, respectively, their diagnostic sensitivities and specificities for distinguishing well differentiated HCC from moderately differentiated and poorly differentiated HCC were 73.3%, 85.5%, 86.7%, and 78.2%, and their areas under the ROC curve were 0.821 and 0.841, respectively. ADC and D values can be used preoperatively to predict the degree of histologic differentiation in HCC, and the D value has better diagnostic value.
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http://dx.doi.org/10.1038/s41598-021-89784-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129092PMC
May 2021

Exosomal lncRNA TCONS_00064356 derived from injured alveolar epithelial type II cells affects the biological characteristics of mesenchymal stem cells.

Life Sci 2021 Aug 5;278:119568. Epub 2021 May 5.

Department of Respiratory Medicine, XinHua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200090, China. Electronic address:

Chronic obstructive pulmonary disease (COPD) is a prevalent respiratory disease, and a leading cause of morbidity and mortality worldwide. There is still a lack of effective treatment to improve pulmonary structural abnormality and reverse the progression of COPD. Mesenchymal stem cell (MSC)-based therapies have attracted much attention and show promising clinical application prospects in COPD treatment. Understanding the communication between injured alveolar cells and MSCs will help us improve the efficiency of MSC-based therapies. Here, we showed that exosomes secreted by injured alveolar epithelial type II (AEC-II) cells could promote the proliferation and migration of MSCs, accompanied with increased expression levels of genes related to mitochondrial synthesis and transfer. Moreover, we identified 21 significantly dysregulated exosomal lncRNAs (16 upregulated and 5 downregulated) using lncRNA sequencing. In addition, we found that lncRNA TCONS_00064356-overexpressing MSCs showed increased proliferation and migration capacities and upregulated expression levels of the genes related to mitochondrial synthesis and transfer. Together, our study uncovers a new potential exosome-mediated communication pathway between injured AEC-II cells and MSCs and provides new targets and ideas for improving the efficiency of MSC-based therapies for COPD.
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http://dx.doi.org/10.1016/j.lfs.2021.119568DOI Listing
August 2021

A case of idiopathic membranous nephropathy and psoriasis vulgaris.

Authors:
Juan Peng Ling Cao

Int Urol Nephrol 2021 Apr 10. Epub 2021 Apr 10.

Department of Nephrology, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, Sichuan, People's Republic of China.

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http://dx.doi.org/10.1007/s11255-021-02851-5DOI Listing
April 2021

Effects of perioperative saphenous and sciatic nerve blocks, lumbosacral epidural or morphine-lidocaine-ketamine infusion on postoperative pain and sedation in dogs undergoing tibial plateau leveling osteotomy.

Vet Anaesth Analg 2021 May 4;48(3):415-421. Epub 2021 Mar 4.

Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA. Electronic address:

Objective: To compare the quality of postoperative analgesia and sedation after preoperative saphenous and sciatic nerve blockade, preoperative lumbosacral epidural injection and perioperative intravenous (IV) morphine, lidocaine and ketamine infusions in dogs undergoing stifle arthroscopy and tibial plateau leveling osteotomy (TPLO) under general anesthesia.

Study Design: Prospective, blinded, randomized, clinical comparison study.

Animals: A total of 45 dogs weighing 33.9 (15.9-56.7) kg and aged 5.2 (1.0-12.0) years, mean (range), undergoing elective unilateral TPLO for spontaneous cranial cruciate ligament rupture.

Methods: Client-owned dogs were enrolled. Dogs were randomly assigned to one of three groups: group MLK, perioperative IV morphine, lidocaine and ketamine infusion; group EPID, lumbosacral epidural with ropivacaine and morphine; or group SSNB, saphenous and sciatic nerve blockade with ropivacaine. Routine stifle arthroscopy followed by TPLO surgery was performed. Sedation and pain scores were assessed at 0, 2, 4, 8 and 24 hours following extubation. Rescue analgesia was administered as prescribed by Glasgow composite pain score-short form score >5.

Results: Sedation scores for MLK were higher than EPID and SSNB. Pain scores for SSNB were lower than those for EPID and MLK. No significant differences were found in anesthesia duration or surgery duration among groups. No dogs required rescue analgesia.

Conclusions And Clinical Relevance: Although analgesia was adequate in all groups, the best combination of analgesia without increased sedation was recorded for SSNB.
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http://dx.doi.org/10.1016/j.vaa.2021.02.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107141PMC
May 2021

Career choice and influential factors among medical students majoring in psychiatry in China.

BMC Med Educ 2021 Mar 25;21(1):183. Epub 2021 Mar 25.

Brain Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.

Background: The undergraduate program of psychiatry has been widely established in recent years to improve the education and recruitment of psychiatrists in China. We aim to investigate the career choice of medical students majoring in psychiatry in China and the influential factors.

Method: This multicenter study was conducted in 26 medical schools in China from May to October of 2019. Participants included 4610 medical students majoring in psychiatry and 3857 medical students majoring in clinical medicine. Multivariable logistic regression was used to investigate the influential factors of students' choices of psychiatry at matriculation and as a career.

Results: 44.08% of psychiatry majored students gave psychiatry as a first choice at matriculation, and 56.67% of them would choose psychiatry as a career, which was in sharp contrast to the proportion of clinical medicine majored students who would choose psychiatry as a career (0.69%). Personal interest (59.61%), suggestions from family members (27.96%), and experiencing mental problems (23.19%) were main reasons for choosing psychiatry major at matriculation. Personal interest (odds ratio [OR] = 2.12, 95% confidence interval [CI] = 1.87-2.40), experiencing a psychiatry clerkship (OR = 1.99, 95% CI = 1.28-3.08), being female (OR = 1.50, 95% CI = 1.30-1.68), experiencing mental problems (OR = 1.33, 95% CI = 1.28-1.56), and suggestions from family members (OR = 1.25, 95% CI = 1.08-1.46) correlated positively with students' choice of psychiatry as career. Students who lacked psychiatry knowledge (OR = 0.49, 95% CI = 0.29-0.85) or chose psychiatry because of lower admission scores (OR = 0.80, 95% CI = 0.63-0.97) were less likely to choose psychiatry as a career.

Conclusion: More than half of psychiatry majored medical school students planned to choose psychiatry as their career, whereas very few students in the clinic medicine major would make this choice. Increasing students' interest in psychiatry, strengthening psychiatry clerkships, and popularizing psychiatric knowledge are modifiable factors to increase the psychiatry career intention. The extent to which medical students' attitudes toward psychiatry can be changed through medical school education and greater exposure to psychiatry will need further investigation.
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http://dx.doi.org/10.1186/s12909-021-02622-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992123PMC
March 2021

Quasi-Contextualized Speech Treatment in Traumatic Brain Injury Inpatient Rehabilitation: Effects on Outcomes During the First Year After Discharge.

J Head Trauma Rehabil 2021 Feb 22. Epub 2021 Feb 22.

Division of Rehabilitation Psychology, Department of Physical Medicine and Rehabilitation, The Ohio State University College of Medicine, Columbus (Drs Beaulieu, Corrigan, and Bogner); Department of Biomedical Informatics, Center for Biostatistics, The Ohio State University Wexner Medical Center, Columbus (Dr Hade and Ms Peng); Department of Rehabilitation Services, The Ohio State University Medical Center, Columbus (Ms Montgomery); Division of Health System Innovation and Research, Department of Population Health Sciences, University of Utah School of Medicine, Salt Lake City (Dr Horn); and Speech Pathology Department, Intermountain Medical Center, Murray, Utah (Ms Gilchrist).

Objective: To evaluate the effect of providing quasi-contextualized speech therapy, defined as metacognitive, compensatory, or strategy training applied to cognitive and language impairments to facilitate the performance of future real-life activities, on functional outcomes up to 1 year following traumatic brain injury (TBI).

Setting: Acute inpatient rehabilitation.

Participants: Patients enrolled during the TBI-Practice-Based Evidence (TBI-PBE) study (n = 1760), aged 14 years or older, who sustained a severe, moderate, or complicated mild TBI, received speech therapy in acute inpatient rehabilitation at one of 9 US sites, and consented to follow-up 3 and 9 months postdischarge from inpatient rehabilitation.

Design: Propensity score methods applied to a database consisting of multisite, prospective, longitudinal observational data.

Main Measures: Participation Assessment with Recombined Tools-Objective-17, FIM Motor and Cognitive scores, Satisfaction With Life Scale, and Patient Health Questionnaire-9.

Results: When at least 5% of therapy time employed quasi-contextualized treatment, participants reported better community participation during the year following discharge. Quasi-contextualized treatment was also associated with better motor and cognitive function at discharge and during the year after discharge. The benefit, however, may be dependent upon a balance of rehabilitation time that relied on contextualized treatment.

Conclusions: The use of quasi-contextualized treatment may improve outcomes. Care should be taken, however, to not provide quasi-contextualized treatment at the expense of contextualized treatment.
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http://dx.doi.org/10.1097/HTR.0000000000000649DOI Listing
February 2021

Large-Scale Rapid Positioning of Hierarchical Assemblies of Conjugated Polymers via Meniscus-Assisted Self-Assembly.

Angew Chem Int Ed Engl 2021 May 12;60(21):11751-11757. Epub 2021 Apr 12.

School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, GA, 30332, USA.

Rapid and deliberate patterning of nanomaterials over a large area is desirable for device manufacturing. We report a method for meniscus-assisted self-assembly (MASA)-enabled rapid positioning of hierarchically assembled dots and stripes composed of luminescent conjugated polymer over two length scales. Periodically arranged conjugated poly(9,9-dioctylfluorene) (PFO) polymers, yield dots, punch-holes and stripes at microscopic scale via MASA. Concurrent self-assembly of PFOs into two-dimensional lenticular crystals within each dot, punch-hole and stripe is realized at nanoscopic scale. Hierarchical assembly is achieved by constraining the evaporation of the PFOs solution in two approximately parallel plates via a MASA process. The three-phase contact line (TCL) of the liquid meniscus of the PFOs was printed using the upper plate, yielding an array of curved stripes. Rapid creation of hierarchical assemblies via MASA opens up possibilities for large-scale organization of a wide range of soft matters and nanomaterials.
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http://dx.doi.org/10.1002/anie.202101272DOI Listing
May 2021

The gut reaction to couples' relationship troubles: A route to gut dysbiosis through changes in depressive symptoms.

Psychoneuroendocrinology 2021 Mar 14;125:105132. Epub 2021 Jan 14.

Institute for Behavioral Medicine Research, The Ohio State University College of Medicine, Columbus, OH, USA; Department of Pediatrics, The Ohio State College of Medicine and Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital, Columbus, OH, USA.

The gut microbiota plays a role in a wide range of diseases and disorders, with low microbial diversity and richness emerging as notable risk factors. This longitudinal study addressed the impact of marital quality (assessed by the Couples Satisfaction Index) on changes in depressive symptoms, and gut diversity, richness, and permeability. On two occasions an average of 90 days apart, 162 people provided stool and blood samples, and completed questionnaires. Depressive symptoms, assessed by the Center for Epidemiological Studies Depression Scale (CES-D), increased from visit 1 to visit 2 in those with clinically significant relationship problems, in contrast to the lack of change among their more satisfied counterparts. These changes in depression were consequential: the gut microbiota's diversity and richness decreased in tandem with the increase in depressive symptoms. Lower relationship satisfaction also foreshadowed increases in lipopolysaccharide binding protein from visit 1 to visit 2, reflecting greater translocation of bacterial endotoxin from the gut to blood circulation, a process that fuels inflammation. Lower diversity and richness provide a pathway from depressive symptoms and marital distress to subsequent health risk.
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http://dx.doi.org/10.1016/j.psyneuen.2021.105132DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7904664PMC
March 2021

Synthesis of PDA-Mediated Magnetic Bimetallic Nanozyme and Its Application in Immunochromatographic Assay.

ACS Appl Mater Interfaces 2021 Jan 21;13(1):1413-1423. Epub 2020 Dec 21.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.

Immunochromatographic assay (ICA) is widely applied in various fields. However, severe matrix interference and weak signal output present major challenges in achieving accurate and ultrasensitive detection in ICA. Here, a polydopamine (PDA)-mediated magnetic bimetallic nanozyme ([email protected]@Pd/Pt) with peroxidase-like activity was synthesized and used as a probe in ICA. The magnetic property of [email protected]@Pd/Pt enabled effective magnetic enrichment of targets, thereby reducing the matrix interference in the sample. PDA coating on the magnetic bimetallic nanozyme was employed as a mediator and a stabilizer. It improved the catalytic ability and stability of the magnetic bimetallic nanozyme by providing more coordination sites for Pd/Pt growth and functional groups (-NH and -OH). In addition, the Pd/Pt bimetallic synergistic effect could further enhance the catalytic ability of the nanozyme. A method was developed by integrating FeO, PDA, and Pd/Pt into [email protected]@Pd/Pt as a probe in ICA. With the proposed method, human chorionic gonadotropin and O157:H7 were successfully detected to be as low as 0.0094 mIU/mL in human blood serum and 9 × 10 CFU/mL in the milk sample, respectively. This method may be readily adapted for accurate and ultrasensitive detection of other biomolecules in various fields.
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http://dx.doi.org/10.1021/acsami.0c17957DOI Listing
January 2021

Cocrystallization-Promoted Charge Mobility in All-Conjugated Diblock Copolymers for High-Performance Field-Effect Transistors.

ACS Appl Mater Interfaces 2020 Dec 17;12(52):58094-58104. Epub 2020 Dec 17.

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200438, China.

The cocrystallization method that combines various constituents into cocrystals yields the newly formed materials with significantly enhanced charge transport properties. However, this strategy has not been greatly utilized in all-conjugated block copolymers (BCPs). Herein, we scrutinize the relationship between cocrystals and charge mobilities in all-conjugated BCPs (i.e., poly(3-butylthiophene)--poly(3-hexylthiophene); denoted P3BT--P3HT) by tuning their molecular weights and thermal annealing process. All the rod-rod BCPs form cocrystals with high charge mobilities than P3BT and P3HT homopolymers and P3BT/P3HT blend, imparting the cocrystal-facilitated charge transport because of the synergy of two conjugated components. Upon 150 °C treatment, their crystallinities increase and their charge mobilities at 15k, 18k, and 28k increase slightly. In contrast, P3BT--P3HT-12k shows decreased charge mobilities. It is due to the preferential increase of crystal size and order through the π-π stacking direction in the former while through the alkyl stacking direction in the latter. Intriguingly, when these P3BT--P3HT cocrystals experience two-step thermal treatment, P3BT--P3HT-12k retains its cocrystalline structure, while microphase separation of P3BT and P3HT occurs in P3BT--P3HT-15k, 18k, and 28k with different degrees. All P3BT--P3HT BCPs exhibit decreased charge mobilities. This study demonstrates the cocrystallization-promoted charge mobility in all-conjugated BCPs, which may facilitate their application in a wide range of optoelectronic devices.
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http://dx.doi.org/10.1021/acsami.0c17671DOI Listing
December 2020

Different functional connectivity modes of the right fronto-insular cortex in akinetic-rigid and tremor-dominant Parkinson's disease.

Neurol Sci 2020 Nov 24. Epub 2020 Nov 24.

Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China.

Background: Patients with akinetic-rigid Parkinson's disease (AR-PD) are more prone to cognitive decline and depressive symptoms than tremor-dominant PD (TD-PD) patients. The right fronto-insular cortex (rFIC), as a key node of salience network, plays a critical role in the switching between central executive network and default mode network. In this study, we explored the functional connectivity mode of rFIC with triple-brain networks, namely default mode network, salience network, and central executive network, in two motor subtypes of PD.

Methods: We recruited 44 PD patients (including the TD-PD group and AR-PD group) and 18 age-matched healthy controls (HCs). We performed functional connectivity (FC) analysis of resting-state functional MRI.

Results: Compared with TD-PD, decreased FC were found in the right insular cortex and bilateral anterior cingulate gyrus in AR-PD. Compared with HCs, decreased FC in the bilateral insula, the anterior cingulate gyrus, the precentral gyrus, and the right medial frontal gyrus were found; therein, the FC value of rFIC-precentral gyrus was positively correlated with the Unified Parkinson's Disease Rating Scale-II score in AR-PD (p = 0.0482, r = 0.4162). While TD-PD showed decreased FC in the left insula as well as bilateral anterior cingulate gyrus when compared with HCs, and the FC value of the rFIC-left insula was positively correlated with its Hamilton Depression Rating Scale score (p = 0.02, r = 0.50).

Conclusion: The functional connectivity mode of rFIC in AR-PD differed from that in TD-PD. The decreased rFIC FC with the other nodes of salience network might be a potential indicator for AR-PD patients prone to develop cognitive decline and depressive symptoms.
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http://dx.doi.org/10.1007/s10072-020-04917-1DOI Listing
November 2020

Shionone alleviates NLRP3 inflammasome mediated pyroptosis in interstitial cystitis injury.

Int Immunopharmacol 2021 Jan 19;90:107132. Epub 2020 Nov 19.

Li Shicai School Inheritance Studio, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, Jiangsu 215101, China. Electronic address:

Shionone is a triterpenoid component derived from the herbal medicine Aster tataricus, and it has been reported to possess marked anti-inflammatory properties. The activation of NLRP3 inflammasome plays an important role in cystitis, and the effect of Shionone on NLRP3 inflammasome-dependent pyroptosis remains unclear. In this study, we established an interstitial cystitis (IC) rat model and SV-HUC-1 cell model with CYP or LPS + ATP treatment to mimic inflammation response and induce NLRP3 inflammasome activation. Shionone treatment significantly attenuated the bladder wet weight, score of edema and hemorrhage, enhanced the viability of SV-HUC-1 cell, decreased the rate of pyroptosis. Moreover, Shionone reduced the expression of NF-κB, NLRP3, ASC, Pro-caspase-1, Caspase-1, GSDMD, GSDMD-N at the mRNA and protein levels both in rat and SV-HUC-1 cell model, demonstrating NLRP3 inflammasome pathway was blocked and pyroptosis degree was reduced. These results indicated that Shionone could alleviate interstitial cystitis in Rat model and enhancing the viability of SV-HUC-1 cells via NF-κB/NLRP3/GSDMD-N pathway, which illustrated that Shionone could be used as a drug candidate for the treatment of interstitial cystitis.
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http://dx.doi.org/10.1016/j.intimp.2020.107132DOI Listing
January 2021

Childhood abuse histories predict steeper inflammatory trajectories across time.

Brain Behav Immun 2021 01 7;91:541-545. Epub 2020 Nov 7.

Institute for Behavioral Medicine Research, The Ohio State University College of Medicine, Columbus, OH, United States; Department of Psychiatry and Behavioral Health, The Ohio State University College of Medicine, Columbus, OH, United States.

Background: The Center for Disease Control (CDC) recently named childhood abuse histories as a public health risk. Clear links between abuse histories and inflammation exist. However, it remains unknown how abuse histories impact inflammatory trajectories throughout adulthood. Accordingly, this study assessed inflammatory trajectories across three visits among healthy adults with and without abuse histories.

Method: In this secondary analysis of data from a longitudinal observational study of cancer survivors and noncancer controls, 157 noncancer controls (M = 55.8, range = 32-83) completed the Childhood Experiences Questionnaire (CTQ), providing data on physical, emotional, and sexual abuse prior to age 18. Cytokines interleukin-6 (IL-6), interleukin 1-beta (IL-1β), and tumor necrosis factor-alpha (TNF-α) were collected at the baseline visit and two follow-up visits approximately one (M months = 11.52, SD = 4.10) and two years (M months = 23.79, SD = 4.40) later. To represent inflammatory changes, cytokine data at each visit were combined into a composite z-score. Covariates in all analyses included age, biological sex, race, income, body mass index, menopause status, psychological diagnosis history, and medical comorbidities.

Results: Compared to their nonabused peers, those who had experienced any type of abuse in childhood demonstrated steeper rises in inflammation across time. Inflammation rose more steeply for individuals with physical and emotional abuse histories compared to those without such histories.

Conclusion: Overall, these data suggest that childhood abuse histories may quicken age-related increases in inflammation, contributing to accelerated aging, morbidity, and early mortality. These findings provide mechanistic insight into why child abuse is a public health risk.
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http://dx.doi.org/10.1016/j.bbi.2020.11.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063138PMC
January 2021

Feasibility of implementing a text-based symptom-monitoring program of endometrial, ovarian, and breast cancer patients during treatment.

Qual Life Res 2020 Oct 14. Epub 2020 Oct 14.

Division of Cancer Prevention and Control, College of Medicine, The Ohio State University, 1590 N. High St, Suite 525, Columbus, OH, 43201, USA.

Purpose: To evaluate the feasibility of implementing systematic patient symptom monitoring during treatment using a smartphone.

Methods: Endometrial [n = 50], ovarian [n = 70] and breast [n = 193] cancer patients participated in text-based symptom reporting for up to 12 months. In order to promote equity, patients without a smartphone were provided with a device, with the phone charges paid by program funds. Each month, patients completed the Patient Health Questionnaire (PHQ-9), and 4 single items assessing fatigue, sleep quality, pain, and global quality of life during the past 7 days rated on a 0 (low) -10 (high) scale. Patients' responses were captured using REDCap, with oncologists receiving monthly feedback. Lay navigators provided assistance to patients with non-medical needs.

Results: Patients utilizing this voluntary program had an overall mean age of 60.5 (range 26-87), and 85% were non-Hispanic white. iPhones were provided to 42 patients, and navigation services were used by 69 patients. Average adherence with monthly surveys ranged between 75-77%, with breast patients having lower adherence after 5 months. The most commonly reported symptoms across cancer types were moderate levels (scores of 4-7) of fatigue and sleep disturbance. At 6 months, 71-77% of all patients believed the surveys were useful to them and their health care team.

Conclusions: We established the feasibility of initiating and managing patients in a monthly text-based symptom-monitoring program. The provision of smartphones and patient navigation were unique and vital components of this program.
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http://dx.doi.org/10.1007/s11136-020-02660-wDOI Listing
October 2020

Protective effect of Aster tataricus extract on NLRP3-mediated pyroptosis of bladder urothelial cells.

J Cell Mol Med 2020 11 8;24(22):13336-13345. Epub 2020 Oct 8.

Li Shicai School Inheritance Studio, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China.

Aster tataricus L.f. is a traditional Eastern Asian herbal medicine used for the relief of uroschesis-related illnesses and has been demonstrated clinically to exert satisfied effects. However, the mechanism of its therapeutic action remains unclear. The present study aimed to evaluate the protective mechanism of Aster tataricus extract (ATE) on CYP or LPS + ATP-induced interstitial cystitis (IC), we successfully constructed the induced IC Sprague-Dawley (SD) rat model and IC human urothelium cell (SV-HUC-1) model. The main compounds of ATE were determined by LC-MS. After intervention, the changes on the bladder wall morphology and inflammation were observed in each group. SV-HUC1 cell viability was measured by MTT and double stained with Hoechst 33342 and propidium iodide (PI). The expression levels of NLRP3, Pro-caspase-1, Caspsae-1 p20, GSDMD, GSDMD-N and Cleave-IL-1β in vivo and in vitro in different groups were detected by Western blotting. ATE significantly alleviated oedema and haemorrhage and reduced the inflammation index and histopathological score in SD rat bladder. The results of cell revealed that ATE could improve cell viability and decrease pyroptosis ratio. The expression of NLRP3 and other pyroptosis-related protein was remarkably decreased by ATE both in vivo and in vitro. ATE may be used as an inhibitor of NLRP3 in treating IC. The discovery of NLRP3/Caspase-1/GSDMD-N as a new protective pathway provides a new direction for protecting cell against IC.
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http://dx.doi.org/10.1111/jcmm.15952DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701514PMC
November 2020

Iron facilitates the RetS-Gac-Rsm cascade to inversely regulate protease IV (piv) expression via the sigma factor PvdS in Pseudomonas aeruginosa.

Environ Microbiol 2020 12 13;22(12):5402-5413. Epub 2020 Oct 13.

Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, ShaanXi, 710069, China.

Pseudomonas aeruginosa produces several proteases, such as an elastase (LasB protease), a LasA protease, and protease IV (PIV), which are thought as significant virulence factors during infection. Regulators of LasA and LasB expression have been identified and well characterized; however, the molecular details of this regulation of protease IV (PIV) remained largely unknown. Here, we describe the interaction between protease IV and the RetS/Rsm signalling pathway, which plays a central role in controlling the production of multiple virulence factors and the switch from planktonic to biofilm lifestyle. We show that the expression of piv was reduced in ΔretS or ΔrsmA strain grown under restrictive conditions but was induced in ΔretS or ΔrsmA mutant grown under rich conditions as compared with wild-type parent. We compare the expression of piv under various conditions and found that iron facilitates RetS/Rsm system to lead this inverse regulation. Moreover, we reveal that the RetS/Rsm pathway regulates PIV production dependent on the alternative sigma factor PvdS. Collectively, this study extends the understanding of the RetS/Rsm regulatory cascade in response to environmental signals and provides insights into how P. aeruginosa adapts to the complex conditions.
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http://dx.doi.org/10.1111/1462-2920.15270DOI Listing
December 2020

Role of Non-Perfusion Factors in Mildly Symptomatic Large Vessel Occlusion Stroke.

J Stroke Cerebrovasc Dis 2020 Oct 30;29(10):105172. Epub 2020 Jul 30.

The Ohio State University Wexner Medical Center, Department of Neurology, 410 W 10th Ave, Columbus, USA.

Introduction: Uncertainty regarding reperfusion of mildly-symptomatic (minor) large vessel occlusion (LVO)-strokes exists. Recently, benefits from reperfusion were suggested. However, there is still no strong data to support this. Furthermore, a proportion of those patients don't improve even after non-hemorrhagic reperfusion. Our study evaluated whether or not non-perfusion factors account for such persistent deconditioning.

Methods: Patients with identified minor LVO-strokes (NIHSS ≤ 8) from our stroke alert registry between January-2016 and May-2018 were included. Variables/ predictors of outcome were tested using univariate/multivariate logistic and linear regression analyses. Three month-modified ranking scale (mRS) was used to differentiate between favorable (mRS = 0-2) and unfavorable outcomes (mRS = 3-6).

Results: Eighty-one patients were included. Significant differences between the two outcome groups regarding admission-NIHSS and discharge-NIHSS existed (OR = 0.47, 0.49 / p = 0.0005, <0.0001 respectively).The two groups had matching perfusion measures. In the poor outcome group, discharge-NIHSS was unchanged from the admission-NIHSS while in the good outcome group, discharge-NIHSS significantly improved.

Conclusion: Admission and discharge NIHSS are independent predictors of outcome in patients with minor-LVO strokes. Unchanged discharge-NIHSS predicts worse outcomes while improved discharge-NIHSS predicts good outcomes. Unchanged NIHSS in the poor outcome group was independent of the perfusion parameters. In literature, complement activation and pro-inflammatory responses to ischemia might account for the progression of stroke symptoms in major-strokes. Our study concludes similar phenomena might be present in minor-strokes. Therefore, discharge-NIHSS may be useful as a clinical marker for future therapies.
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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2020.105172DOI Listing
October 2020

Reliable performance of aggregation-induced emission nanoparticle-based lateral flow assay for norfloxacin detection in nine types of animal-derived food.

Talanta 2020 Nov 6;219:121245. Epub 2020 Jul 6.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, China. Electronic address:

The wide application of lateral flow assay (LFA) was limited by its low sensitivity and poor matric tolerance. Aggregation induced emission (AIE) materials show superior luminescence and good stability under close packing state, which may accelerate the development of LFA. However, the detection performance of the AIE-based LFA in different real samples was unclear. In this work, an AIE-LFA was established for norfloxacin (NOR) detection in nine types of animal-derived food. Results indicated that AIE-LFA had the average recovery range of 75.6%-95.1%, 78.6%-94.6%, 71.4%-112.7%, 81.7%-121.8%, 72.7%-93.5%, 79.8%-108.5%, 79.2%-109.4%, 76.3%-103.6%, and 80.6%-108.3% in pork, pig liver, fish, lamb, beef, milk, chicken, egg, and honey, respectively. The detection results of AIE-LFA were compatible with HPLC-MS/MS in detecting NOR in 135 real samples from nine types of animal-derived food. The developed AIE-LFA was sensitive and reliable for NOR detection in those real samples.
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http://dx.doi.org/10.1016/j.talanta.2020.121245DOI Listing
November 2020

Author Correction: Requirement for the basic helix-loop-helix transcription factor Dec2 in initial TH2 lineage commitment.

Nat Immunol 2020 Nov;21(11):1467

Department of Immunology, MD Anderson Cancer Center, Houston, Texas, USA.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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http://dx.doi.org/10.1038/s41590-020-0787-1DOI Listing
November 2020

Immunochromatographic assay based on time-resolved fluorescent nanobeads for the rapid detection of sulfamethazine in egg, honey, and pork.

J Sci Food Agric 2021 Jan 21;101(2):684-692. Epub 2020 Aug 21.

School of Food Science and Technology, Nanchang University, Nanchang, China.

Background: Sulfamethazine (SMZ), a veterinary drug widely used in animal husbandry, is harmful to human health when excess residues are present in food. In this study, a fast, reliable, and sensitive immunochromatographic assay (ICA) was developed on the basis of the competitive format by using time-resolved fluorescent nanobeads (TRFN) as label for the detection of SMZ in egg, honey, and pork samples.

Results: Under optimized working conditions, this method had limits of detection of 0.016, 0.049, and 0.029 ng mL and corresponding linear ranges of 0.05 to 1.00, 0.05 to 5.00, and 0.05 to 1.00 ng mL in egg, honey, and pork samples, respectively. The recovery experiments showed that the average recoveries ranged from 90.5% to 113.9%, 82.4% to 112.0%, and 79.8% to 93.4% with corresponding coefficients of variation of 4.1% to 11.7%, 7.5% to 11.5%, and 4.8% to 8.7% for egg, honey, and pork samples, respectively. The developed TRFN-ICA was also systematically compared with high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) by analyzing 45 actual samples from egg, honey, and pork.

Conclusion: Overall, the developed TRFN-ICA had high reliability and excellent potential for the ultrasensitive detection of SMZ for food safety monitoring, also providing a universal platform for the on-site detection of other targets. © 2020 Society of Chemical Industry.
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http://dx.doi.org/10.1002/jsfa.10681DOI Listing
January 2021

Sensitive immunoassays based on a monoclonal antibody for detection of marbofloxacin in milk.

J Dairy Sci 2020 Sep 16;103(9):7791-7800. Epub 2020 Jul 16.

School of Food Science, Nanchang University, Nanchang 330047, China. Electronic address:

Marbofloxacin (MBF) is a key class of synthetic fluoroquinolone antibiotic that is commonly used as a veterinary drug. However, excess residue of MBF in animal-derived food samples, such as milk, is harmful to consumers. In this study, 4 mAb against MBF, namely, M4E3, M7A6, M3C7, and M5C6, were produced. Indirect competitive (ic) ELISA revealed that the M4E3 exhibited the highest sensitivity with a half-maximal inhibitory concentration (IC) of 0.07 ng/mL and a limit of detection of 0.01 ng/mL for detection of MBF. The results of the cross-reactivity experiment revealed that the M4E3 could detect lomefloxacin, ofloxacin, fleroxacin, pefloxacin, danofloxacin, and enrofloxacin sensitively with IC of 0.02, 0.07, 0.18, 0.27, 0.30, and 0.32 ng/mL, respectively. Meanwhile, a cross-reactivity experiment showed that the M4E3 had a crossover rate of more than 20% with these fluoroquinolone analogs. A weak crossover rate below 1.11% was observed with 14 other fluoroquinolone analogs. The recovery rate of MBF in milk ranged from 72.28% to 129.19%, which showed that the developed indirect competitive ELISA was effective in detecting MBF in milk. Furthermore, a visual colloidal gold-based immunochromatographic assay was developed for detecting MBF with a cut-off value of 1 ng/mL in both phosphate-buffered saline and a milk sample by using this mAb. Given this sensitive mAb, both indirect competitive ELISA and colloidal gold-based immunochromatographic assay could be effective screening tools for detection of MBF in milk.
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http://dx.doi.org/10.3168/jds.2019-18108DOI Listing
September 2020

Bifunctional Electrocatalyst with 0D/2D Heterostructure for Highly Efficient Hydrogen and Oxygen Generation.

Chem Asian J 2020 Sep 8;15(18):2892-2899. Epub 2020 Sep 8.

School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.

A novel MoS quantum dots/CoSe nanosheet (MoS QDs/CoSe ) hybrid with 0D/2D heterostructure has been developed. The CoSe nanosheets (NSs) enable an excellent oxygen evolution reaction (OER) activity with increasing vacancy configuration on one hand, while the MoS QDs serve as an eminent hydrogen evolution reaction (HER) catalyst on the other. By integrating MoS QDs and CoSe NSs, the hybrid exhibits excellent electrocatalytic performances in HER and OER. The unique 0D/2D hetero-interface increases the exposed active sites and facilitates electron transfer, thereby boosting the electrocatalytic activity. Relatively low overpotentials of 82 mV and 280 mV are required to drive the current density of 10 mA/cm for HER and OER, with corresponding Tafel slopes of 69 and 75 mV/dec, respectively. As such, this work provides an efficient yet simple approach to construct bifunctional electrocatalysts with enhanced activity and stability.
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http://dx.doi.org/10.1002/asia.202000419DOI Listing
September 2020

For better and worse? The roles of closeness, marital behavior, and age in spouses' cardiometabolic similarity.

Psychoneuroendocrinology 2020 10 17;120:104777. Epub 2020 Jun 17.

Institute for Behavioral Medicine Research, The Ohio State University Wexner Medical Center, United States; Department of Psychiatry and Behavioral Health, OSUWMC, United States.

Spouses share common risks for cardiometabolic diseases: a person's diabetes or hypertension raises the partner's odds of developing the same condition. The mechanisms responsible for this disease concordance remain poorly understood. To examine three factors that may modulate partners' cardiometabolic similarity-closeness, hostile marital behavior, and age-and to explore whether health behavior concordance plays a role, on two separate occasions 43 healthy couples ages 24-61 provided fasting glucose, metabolic data (fat and carbohydrate oxidation), and resting blood pressure before discussing one of their most severe marital disagreements. Accounting for the fixed effects of sex, age, study visit, and abdominal fat on cardiometabolic levels, we found that aspects of health behavior concordance were associated with greater similarity in glucose, diastolic blood pressure (DBP), and carbohydrate and fat metabolism. Independent of health behavior concordance, partners who felt closer and behaved in a less hostile way had more similar rates of fat oxidation; less hostile partners also shared greater overlap in carbohydrate oxidation. Likewise, fasting glucose and DBP were more similar within older couples compared to younger pairs, beyond the effects of health behavior concordance. In sum, our data captured preclinical similarities in cardiometabolic health among disease-free couples, which may form the basis for their long-term overlapping disease risks. Closer, less hostile, and older couples shared more similar fasting glucose, metabolic data, and blood pressure; importantly, health behavior concordance did not explain all associations. These novel data suggest that multiple paths may lead to couples' shared disease risks.
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http://dx.doi.org/10.1016/j.psyneuen.2020.104777DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849824PMC
October 2020

Dual-mode immunoassay system based on glucose oxidase-triggered Fenton reaction for qualitative and quantitative detection of danofloxacin in milk.

J Dairy Sci 2020 Sep 26;103(9):7826-7833. Epub 2020 Jun 26.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address:

In this study, a novel colorimetric and fluorescent dual-mode ELISA based on glucose oxidase (GOx)-triggered Fenton reaction was developed for the qualitative and quantitative detection of danofloxacin (DAN). In this system, streptavidin-linked biotinylated anti-DAN-monoclonal antibody (SA-Bio-mAb) and biotinylated GOx (Bio-GOx) form the immune complex mAb-Bio-SA-Bio-GOx. In the absence of DAN, the mAb-Bio-SA-Bio-GOx would be immobilized by combining with coated DAN-BSA and catalyzed glucose to generate HO. The Fenton reaction between HO and Fe generated hydroxyl radicals, which oxidized the o-phenylenediamine to 2,3-diamino-phenazine. A dual-signal immunoassay with colorimetry and fluorescence as the signal readout was established. In the presence of DAN, DAN and DAN-BSA competed with Bio-mAb, decreasing the connection between immune complexes and DAN-BSA and finally resulting in lower signal of colorimetry and fluorescence. Under optimal conditions, the limit of detection of the fluorescence immunoassay was 0.337 ng/mL and was 5.24-fold lower than that of traditional ELISA. The colorimetric immunoassay cut-off value was 30 ng/mL in milk. The average recoveries of the method for milk samples that are spiked with different concentrations of DAN were 91.1 to 128.3%, with a coefficient of variation of 0.7 to 8.2%. These results of the method exhibited good agreement with those of liquid chromatography-tandem mass spectrometry system (LC-MS/MS) method. In brief, this work provides an improved screening strategy with high sensitivity and accuracy for the qualitative or quantitative detection of DAN in milk monitoring.
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http://dx.doi.org/10.3168/jds.2020-18256DOI Listing
September 2020

Biochar amendment as a remediation strategy for surface soils impacted by crude oil.

Environ Pollut 2020 Oct 11;265(Pt B):115006. Epub 2020 Jun 11.

Department of Land, Air and Water Resources, One Shields Ave., University of California Davis, Davis, CA, 95616, USA. Electronic address:

The impact of organic bulking agents on the biodegradation of petroleum hydrocarbons in crude oil impacted soils was evaluated in batch laboratory experiments. Crude oil impacted soils from three separate locations were amended with fertilizer and bulking agents consisting of biochars derived from walnut shells or ponderosa pine wood chips produced at 900 °C. The batch reactors were incubated at 25 °C and sampled at pre-determined intervals to measure changes in total petroleum hydrocarbons (TPH) over time. For the duration of the incubation, the soil moisture content was adjusted to 75% of the maximum water holding capacity (MWHC) and prior to each sampling event, the sample was manually stirred. Results show that the addition of fertilizer and bulking agents increased biodegradation rates of TPH. Soil samples amended with ponderosa pine wood biochar achieved the highest biodegradation rate, whereas the walnut shell biochar was inhibitory to TPH biodegradation. The beneficial impact of biochars on TPH biodegredation was more pronounced for a soil impacted with lighter hydrocarbons compared to a soil impacted with heavier hydrocarbons. This study demonstrates that some biochars, in combination with fertilizer, have the potential to be a low-technology and eco-friendly remediation strategy for crude oil impacted soils.
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http://dx.doi.org/10.1016/j.envpol.2020.115006DOI Listing
October 2020

Gold nanorods etching-based plasmonic immunoassay for qualitative and quantitative detection of aflatoxin M1 in milk.

Food Chem 2020 Nov 27;329:127160. Epub 2020 May 27.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China. Electronic address:

In this study, we developed an indirect competitive plasmonic immunoassay using glucose oxidase (GOx)-induced redox reaction to etch Au nanorods (AuNRs) for qualitative and quantitative detection of aflatoxin M1 (AFM1) in milk. In this system, streptavidin (SA) was selected as a linker between biotinylated anti-AFM1-monoantibody (bio-mAb) and biotinylated GOx (bio-GOx) to form the immunocomplexes bio-mAb-SA-bio-GOx. After the oxidation of the glucose and I, the resultant I could etch cetytrimethylammonium bromide (CTAB)-stabilized AuNRs. This resulted in the blue shift of the longitudinal localized surface plasmon resonance (LSPR) extinction peak, with a color change from blue to pink. The linear range and limit of detection (LOD) of the plasmonic immunoassay were 0.25-10 ng mL and 0.11 ng mL, respectively. It displayed quantitative detection with high sensitivity, specificity, recovery, and accuracy, which is promising for qualitative and quantitative detection of AFM1 in food safety.
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http://dx.doi.org/10.1016/j.foodchem.2020.127160DOI Listing
November 2020