Publications by authors named "Juan Li"

4,115 Publications

  • Page 1 of 1

Silver-decorated MXene nanosheets as a radical initiator for polymerization and multifunctional hydrogels.

Chem Commun (Camb) 2022 May 26. Epub 2022 May 26.

Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.

Silver nanoparticle-decorated multilayered titanium carbide MXene (Ag/TiCT) itself is capable of initiating the polymerization of a variety of acrylic monomers, due to it being able to generate hydroxyl radicals the pseudo-Fenton reaction. Furthermore, double-network hydrogel Ag/[email protected]/PAAm is synthesized by a one-pot procedure and displays a good near-infrared light-triggered shape-memory performance.
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http://dx.doi.org/10.1039/d2cc00504bDOI Listing
May 2022

Caregiver burden and its associated factors among family caregivers of persons with dementia in Shanghai, China: a cross-sectional study.

BMJ Open 2022 May 24;12(5):e057817. Epub 2022 May 24.

Department of Neurology, Second Affiliated Hospital of Naval Medical University (Changzheng Hospital), Shanghai, China

Objective: To assess the level of caregiver burden and factors associated with it among family caregivers of persons with dementia (PWD) living in communities of Shanghai, China.

Design: Cross-sectional study.

Setting: Communities in Hongkou District of Shanghai, China.

Participants: A random sample of 109 older adults with dementia and their primary family caregivers.

Main Outcome Measure: Caregiver burden measured by the Caregiver Burden Inventory (CBI), and the Caregivers' depressive symptom measured by the simplified Chinese version of Self-rating Depression Scale was the outcome variable of the study. The independent variables, including the cognitive function (measured by Montreal Cognitive Assessment (MoCA), sleep quality assessed by the Pittsburgh Sleep Quality Index, abilities of daily life assessed by the Activities of Daily Living Scale, and behavioural and psychological symptoms assessed by the Neuropsychiatric Inventory of PWDs, the community service utilisation (measured by the Community Service Utilisation Measurement), perceived social support (assessed by three questions), positive aspects of caregiving (PAC) (assessed by the PAC) of dementia caregivers, were analysed. Multivariate linear regression was employed to determine the factors related to caregiver burden.

Results: The average level of CBI was 65.92±16.74. The score of MoCA, PAC and perceived social support of caregivers were negatively associated with caregiver burden (β=-0.84, p<0.001, β=-3.61, p=0.03 and β=-1.22, p=0.001, respectively). Community service utilisation was positively associated (β=3.46, p<0.001) with caregiver burden. Perceived social support by the caregiver moderated the relationship between caregiver burden and caregivers' depression symptoms.

Conclusion: Dementia caregivers experienced a high level of caregiver burden. The cognitive function of PWD, PAC, social support and community service utilisation were factors associated with caregiver burden. Strengthening social support, providing more high-quality home care services, promoting PAC are imperative to reduce caregiver burden.
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http://dx.doi.org/10.1136/bmjopen-2021-057817DOI Listing
May 2022

Worldwide Research Trends on Artemisinin: A Bibliometric Analysis From 2000 to 2021.

Front Med (Lausanne) 2022 6;9:868087. Epub 2022 May 6.

Department of Radiology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China.

Objective: Artemisinin is an organic compound that comes from Artemisia annua. Artemisinin treatment is the most important and effective method for treating malaria. Bibliometric analysis was carried out to identify the global research trends, hot spots, scientific frontiers, and output characteristics of artemisinin from 2000 to 2021.

Methods: Publications and their recorded information from 2000 to 2021 were retrieved through the Web of Science Core Collection (WoSCC). Using VOSviewer and Citespace, the hotspots and trends of studies on artemisinin were visualized.

Results: A total of 8,466 publications were retrieved, and for the past 22 years, the annual number of publications associated with artemisinin kept increasing. The United States published most papers. The H-index and number of citations of the United States ranked first. The University of Oxford and MALARIA JOURNAL were the most productive affiliation and journal, respectively. A paper written by E.A. Ashley in 2011 achieved the highest global citation score. Keywords, such as "malaria," "artesunate," "plasmodium-falciparum," "," "artemisinin resistance," "plasmodium falciparum," "resistance," and "artemether-lumefantrine," appeared most frequently. The research on artemisinin includes clinical research and animal and cell experiments.

Conclusion: The biosynthesis, drug resistance mechanism, and combination of artemisinin have become more popular than before. Studies on artemisinin treating coronavirus disease 2019 (COVID-19) have been carried out, and good research results have been obtained.
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http://dx.doi.org/10.3389/fmed.2022.868087DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9121127PMC
May 2022

Altered Spontaneous Brain Activity in Patients With Diabetic Osteoporosis Using Regional Homogeneity: A Resting-State Functional Magnetic Resonance Imaging Study.

Front Aging Neurosci 2022 6;14:851929. Epub 2022 May 6.

Department of Radiology, The Second Affiliated Hospital of Shandong First Medical University, Taian, China.

Background: The pathophysiological mechanism of cognitive impairment by osteoporosis in type 2 diabetes mellitus (T2DM) remains unclear. This study aims to further investigate the regional spontaneous brain activity changes of patients with diabetic osteoporosis (DOP), and the correlation between abnormal brain regions and bone metabolites.

Methods: A total of 29 subjects with T2DM were recruited, including fourteen patients with DOP and thirteen patients without osteoporosis (Control group). Based on the resting-state functional magnetic resonance imaging (rs-fMRI) datasets acquired from all the subjects, a two-sample -test was performed on individual normalized regional homogeneity (ReHo) maps. Spearman correlation analysis was performed between the abnormal ReHo regions with the clinical parameters and Montreal Cognitive Assessment (MOCA) scores.

Results: In the DOP group, we demonstrated the significantly increased ReHo values in the left middle temporal gyrus (MTG), right superior occipital gyrus (SOG), aright superior parietal lobule (SPL), right angular gyrus (AG), and left precuneus (PE). Additionally, we also found a significant positive correlation between increased ReHo values in the left MTG and the average bone mineral density (BMD AVG), and average T scores (T AVG). The ReHo values of the right SOG and right SPL showed a negative correlation with MOCA scores, as well as a negative correlation between increased ReHo values in the right SPL and osteocalcin (OC) level.

Conclusion: Patients with DOP showed increased spontaneous activity in multiple brain regions. The results indicated that osteoporosis exacerbated cognitive impairment and brain damage. Also, the OC might be considered as a bone marker to track the progression of cognitive impairment.
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http://dx.doi.org/10.3389/fnagi.2022.851929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120436PMC
May 2022

Ginsenoside Rb1 attenuates methamphetamine (METH)-induced neurotoxicity through the NR2B/ERK/CREB/BDNF signalings and models.

J Ginseng Res 2022 May 14;46(3):426-434. Epub 2021 Jul 14.

CUHK-SDU Joint Laboratory on Reproductive Genetics, School of Biomedical Sciences, the Chinese University of Hong Kong, Hong Kong, China.

Aim: This study investigates the effects of ginsenoside Rb1 (GsRb1) on methamphetamine (METH)-induced toxicity in SH-SY5Y neuroblastoma cells and METH-induced conditioned place preference (CPP) in adult Sprague-Dawley rats. It also examines whether GsRb1 can regulate these effects through the NR2B/ERK/CREB/BDNF signaling pathways.

Methods: SH-SY5Y cells were pretreated with GsRb1 (20 μM and 40 μM) for 1 h, followed by METH treatment (2 mM) for 24 h. Rats were treated with METH (2 mg/kg) or saline on alternating days for 10 days to allow CPP to be examined. GsRb1 (5, 10, and 20 mg/kg) was injected intraperitoneally 1 h before METH or saline. Western blot was used to examine the protein expression of NR2B, ERK, P-ERK, CREB, P-CREB, and BDNF in the SH-SY5Y cells and the rats' hippocampus, nucleus accumbens (NAc), and prefrontal cortex (PFC).

Results: METH dose-dependently reduced the viability of SH-SY5Y cells. Pretreatment of cells with 40 μM of GsRb1 increased cell viability and reduced the expression of METH-induced NR2B, p-ERK, p-CREB and BDNF. GsRb1 also attenuated the expression of METH CPP in a dose-dependent manner in rats. Further, GsRb1 dose-dependently reduced the expression of METH-induced NR2B, p-ERK, p-CREB, and BDNF in the PFC, hippocampus, and NAc of rats.

Conclusion: GsRb1 regulated METH-induced neurotoxicity and METH-induced CPP through the NR2B/ERK/CREB/BDNF regulatory pathway. GsRb1 could be a therapeutic target for treating METH-induced neurotoxicity or METH addiction.
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http://dx.doi.org/10.1016/j.jgr.2021.07.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9120644PMC
May 2022

Safety, activity, and pharmacokinetics of camrelizumab in advanced Asian melanoma patients: a phase I study.

BMC Cancer 2022 May 20;22(1):565. Epub 2022 May 20.

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Renal Cancer and Melanoma, Peking University Cancer Hospital & Institute, 52 Fucheng Road, Haidian District, Beijing, 100142, China.

Background: Anti-programmed cell death receptor-1 (PD-1) monotherapy is the standard treatment for metastatic melanoma in current. Camrelizumab is a humanized IgG4 anti-PD-1 monoclonal antibody whose safety and efficacy have not been reported in advanced Asian melanoma patients.

Methods: This phase I study investigated the safety, activity, and pharmacokinetics of camrelizumab in Chinese patients with advanced melanoma. The study included two phases, the dose-escalation phase ("3 + 3" design at 60 mg, 200 mg, and 400 mg) and the dose-expansion phase.

Results: No dose-limiting toxicities were recorded over the dose-escalation phase, and the maximum tolerated dose was not reached. The most common treatment-related adverse events (TRAEs) in 36 patients were reactive cutaneous capillary endothelial proliferation, followed by rash, fever, hypothyroidism, hyperthyroidism, vitiligo, and fatigue. Five grade 3 or above TRAEs were reported (13.9%), including two cases of elevated γ-glutamyltransferase and blood triglycerides without clinical symptoms, and one liver injury recovered after symptomatic treatment. The confirmed overall response rate was 13.9% (95%CI: 4.7, 29.5%) and disease control rate was 38.9% (95%CI: 23.1, 56.5%). The median progression-free survival was 1.8 months (95%CI: 1.1, 2.4) and the median overall survival was 11.1 months (95%CI: 6.8, 15.4).

Conclusions: Camrelizumab had acceptable tolerability and similar anti-tumor activity compared with other anti-PD-1 antibodies in advanced Asian melanoma patients.

Trial Registration: ClinicalTrials.gov identification: NCT02738489. Registered on 14/04/2016, prospectively registered.
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http://dx.doi.org/10.1186/s12885-022-09663-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9123656PMC
May 2022

New Insights Into the Local Auxin Biosynthesis and Its Effects on the Rapid Growth of Moso Bamboo ().

Front Plant Sci 2022 3;13:858686. Epub 2022 May 3.

Key Laboratory of National Forestry and Grassland Administration/Beijing for Bamboo & Rattan Science and Technology, International Center for Bamboo and Rattan, Beijing, China.

Auxin plays a crucial regulatory role in higher plants, but systematic studies on the location of auxin local biosynthesis are rare in bamboo and other graminaceous plants. We studied moso bamboo (), which can grow up to 1 m/day and serves as a reference species for bamboo and other fast-growing species. We selected young tissues such as root tips, shoot tips, young culm sheaths, sheath blades, and internode divisions for local auxin biosynthesis site analysis. IAA immunofluorescence localization revealed that auxin was similarly distributed in different stages of 50-cm and 300-cm bamboo shoots. Shoot tips had the highest auxin content, and it may be the main site of auxin biosynthesis in the early stage of rapid growth. A total of 22 key genes in the YUCCA family for auxin biosynthesis were identified by genome-wide identification, and these had obvious tissue-specific and spatio-temporal expression patterns. hybridization analysis revealed that the localization of genes was highly consistent with the distribution of auxin. Six major auxin synthesis genes, , , Phe, , , and , were obtained that may have regulatory roles in auxin accumulation during moso bamboo growth. Culm sheaths were found to serve as the main local sites of auxin biosynthesis and the auxin required for internode elongation may be achieved mainly by auxin transport.
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http://dx.doi.org/10.3389/fpls.2022.858686DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9111533PMC
May 2022

LINC01232 Promotes Metastasis and EMT by Regulating miR-506-5p/PAK1 Axis in Gastric Cancer.

Cancer Manag Res 2022 13;14:1729-1740. Epub 2022 May 13.

Digestive System Department, Hunan Provincial People's Hospital (The First-Affiliated Hospital of Hunan Normal University), Changsha, People's Republic of China.

Background: Long non-coding RNA LINC01232 plays an important role in the progression of metastasis in several cancers. However, the function of LINC01232 in gastric cancer is limited. Authors aimed to investigate the role and mechanism of LINC01232 in the metastasis of gastric cancer.

Methods: The expression levels and correlation of LINC01232, miR-506-5p, and PAK1 were analyzed by GEPIA or ENCORI, and the abundance of LINC01232 and miR-506-5p was measured in tissues and cells via qRT-PCR, the location of LINC01232 in gastric cells was analyzed by nuclear and cytoplasmic fractionation, while the protein levels of PAK1, E-cadherin and vimentin were additionally quantified by Western blotting. Interactions between LINC01232, miR-506-5p, and PAK1 were detected through luciferase reporter assays, qRT-PCR and Western blotting. Cellular viability was evaluated through CCK8 assays, migration ability was measured by transwell assays, invasion ability was tested by wound healing experiment.

Results: LINC01232 was overexpressed in gastric cancer tissues and cells, and mainly located in nucleus. The inhibition of LINC01232 could suppress migration, invasion and EMT of gastric cancer cells. MiR-506-5p was downregulated in gastric cancer tissues and cells. LINC01232 sponged miR-506-5p to accelerate migration and EMT. PAK1 was certified to be a target of miR-506-5p, inhibition of PAK1 could interrupt LINC01232 overexpression-induced migration of gastric cancer cells.

Conclusion: The LINC01232/miR-506-5p/PAK1 axis promotes metastasis of gastric cancer cells.
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http://dx.doi.org/10.2147/CMAR.S352081DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9113486PMC
May 2022

Zengye Decoction Attenuated Severe Acute Pancreatitis Complicated with Acute Kidney Injury by Modulating the Gut Microbiome and Serum Amino Acid Metabolome.

Evid Based Complement Alternat Med 2022 9;2022:1588786. Epub 2022 May 9.

Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.

Objective: To explore the effect and underlying mechanism of Zengye decoction (ZYD), a traditional formula from China, on the severe acute pancreatitis (SAP) rat model with acute kidney injury (AKI).

Methods: The SAP-AKI model was induced by 3.5% sodium taurocholate. Rats were treated with normal saline or ZYD twice and sacrificed at 36 h after modeling. Amylase, lipase, creatinine, blood urea nitrogen, kidney injury molecule 1(KIM-1), and multiple organs' pathological examinations were used to assess the protective effect of ZYD. Gut microbiome detected by 16S rRNA sequencing analysis and serum amino acid metabolome analyzed by liquid chromatography-mass spectrometry explained the underlying mechanism. The Spearman correlation analysis presented the relationship between microflora and metabolites.

Results: ZYD significantly decreased KIM-1( < 0.05) and the pathological score of the pancreas ( < 0.05), colon ( < 0.05), and kidney ( < 0.05). Meanwhile, ZYD shifted the overall gut microbial structure (-diversity, ANOSIM  = 0.14, =0.025) and altered the microbial compositions. Notably, ZYD reduced the potentially pathogenic bacteria-Bacteroidetes, Clostridiales vadin BB60 group, and uncultured_Clostridiales_bacterium, but promoted the short-chain fatty acid (SCFA) producers-Erysipelotrichaceae, Bifidobacterium, Lactobacillus, and (all < 0.05). Moreover, principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and hierarchical clustering analysis (HCA) presented a remarkable change in amino acid metabolome after SAP-AKI induction and an apparent regulation by ZYD treatment (R2Y 0.878, =0.01; Q2 0.531, =0.01). Spearman's correlation analysis suggested that gut bacteria likely influenced serum metabolites levels (absolute  > 0.4 and FDR < 0.02).

Conclusions: ZYD attenuated SAP-AKI by modulating the gut microbiome and serum amino acid metabolome, which may be a promising adjuvant treatment.
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http://dx.doi.org/10.1155/2022/1588786DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9110161PMC
May 2022

Evidence for Neuropeptide W Acting as a Physiological Corticotropin-releasing Inhibitory Factor (CRIF) in Male Chickens.

Endocrinology 2022 May 18. Epub 2022 May 18.

Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, PR China.

In vertebrates, corticotropin (ACTH), released by the pituitary gland, is a critical part of the stress axis and stress response. Generally, the biosynthesis and secretion of ACTH are controlled by both hypothalamic stimulatory factors and inhibitory factors (e.g., CRIF), but the identity of this CRIF remains unrevealed. We characterized the neuropeptide B (NPB)/neuropeptide W (NPW) system in chickens and found that NPW could directly target the pituitary to inhibit growth hormone and prolactin secretion via neuropeptide B/W receptor 2 (NPBWR2), which is completely different from the mechanism in mammals. Present study first carried out a series of assays to investigate the possibility that NPW acts as a physiological CRIF in chickens. The results showed that: (1) NPW could inhibit ACTH synthesis and secretion by inhibiting the cAMP/PKA signaling cascade in vitro and in vivo; (2) NPBWR2 was expressed abundantly in corticotrophs (ACTH-producing cells), which are located mainly in cephalic lobe of chicken pituitary, as demonstrated by single-cell RNA-sequencing, immunofluorescent staining and fluorescence in situ hybridization; (3) dexamethasone could stimulate pituitary NPBWR2 and hypothalamic NPW expression in chicks, which was accompanied by the decease of POMC mRNA levels, as revealed by in vitro and subcutaneous injection assays; and (4) the temporal expression profiles of NPW-NPBWR2 pair in hypothalamus-pituitary axis and POMC in pituitary were almost unanimous in chicken. Collectively, these findings provide comprehensive evidence for the first time that NPW is a potent physiological CRIF in chickens that plays core roles in suppressing the activity of the stress axis.
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http://dx.doi.org/10.1210/endocr/bqac073DOI Listing
May 2022

AENEAS: A Randomized Phase III Trial of Aumolertinib Versus Gefitinib as First-Line Therapy for Locally Advanced or MetastaticNon-Small-Cell Lung Cancer With Exon 19 Deletion or L858R Mutations.

J Clin Oncol 2022 May 17:JCO2102641. Epub 2022 May 17.

Respiratory Medicine, Xiangya Hospital Central South University, Changsha, China.

Purpose: Aumolertinib (formerly almonertinib; HS-10296) is a novel third-generation epidermal growth factor receptor tyrosine kinase inhibitor approved in China. This double-blind phase III trial evaluated the efficacy and safety of aumolertinib compared with gefitinib as a first-line treatment for locally advanced or metastatic -mutated non-small-cell lung cancer (NSCLC; ClinicalTrials.gov identifier: NCT03849768).

Methods: Patients at 53 sites in China were randomly assigned 1:1 to receive either aumolertinib (110 mg) or gefitinib (250 mg) once daily. The primary end point was progression-free survival (PFS) per investigator assessment.

Results: A total of 429 patients who were naïve to treatment for locally advanced or metastatic NSCLC were enrolled. PFS was significantly longer with aumolertinib compared with gefitinib (hazard ratio, 0.46; 95% CI, 0.36 to 0.60; < .0001). The median PFS with aumolertinib was 19.3 months (95% CI, 17.8 to 20.8) versus 9.9 months with gefitinib (95% CI, 8.3 to 12.6). Objective response rate and disease control rate were similar in the aumolertinib and gefitinib groups (objective response rate, 73.8% and 72.1%, respectively; disease control rate, 93.0% and 96.7%, respectively). The median duration of response was 18.1 months (95% CI, 15.2 to not applicable) with aumolertinib versus 8.3 months (95% CI, 6.9 to 11.1) with gefitinib. Adverse events of grade ≥ 3 severity (any cause) were observed in 36.4% and 35.8% of patients in the aumolertinib and gefitinib groups, respectively. Rash and diarrhea (any grade) were observed in 23.4% and 16.4% of patients who received aumolertinib compared with 41.4% and 35.8% of those who received gefitinib, respectively.

Conclusion: Aumolertinib is a well-tolerated third-generation epidermal growth factor receptor tyrosine kinase inhibitor that could serve as a treatment option for -mutant NSCLC in the first-line setting.
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http://dx.doi.org/10.1200/JCO.21.02641DOI Listing
May 2022

A Chlorine-Based Redox Electrochemical Capacitor.

ACS Appl Mater Interfaces 2022 May 17. Epub 2022 May 17.

Shenyang National Laboratory for Materials Science, Institute of Metal Research, Chinese Academy of Sciences, Shenyang 110016, People's Republic of China.

Electrochemical capacitors are under the spotlight due to their high power density, but they have a low energy density. Redox electrolytes have emerged as a promising approach to design high-energy electrochemical energy storage devices. Herein, a chlorine-based redox electrochemical capacitor is reported in an ionic liquid electrolyte. The commercial activated carbon is employed as the working electrode to render the reversible redox of chloride ions in an ionic liquid, by the restriction of micropores on neutral chlorine. The carbon material can simultaneously provide electrical double-layer capacitance. The effective integration of a chlorine redox reaction and electrical double layer allows for high-energy electrochemical capacitors. By this means, a rechargeable chlorine-based redox electrochemical capacitor with reversible capacity and good rate capability and cycling stability is obtained. This work offers a solution for a new type of high-energy electrochemical capacitors.
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http://dx.doi.org/10.1021/acsami.2c03951DOI Listing
May 2022

Autophagic flux inhibition, apoptosis, and mitochondrial dysfunction in bile acids-induced impairment of human placental trophoblast.

J Cell Physiol 2022 May 17. Epub 2022 May 17.

The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.

Intrahepatic cholestasis of pregnancy (ICP) is a common pregnancy-specific disease, characterized by increased bile acid levels and adverse fetal outcomes. We previously reported excessive bile acids led to dysfunction of placental trophoblasts in ICP. However, the detailed mechanism is still unclear. Autophagy is fundamental process for protecting cell survival against adverse conditions. Here, we evaluated the effect of increased concentration of bile acids on autophagy in trophoblasts in vitro and in vivo. First, we demonstrated that the autophagy substrate p62/sequestosome-1 was accumulated in placental tissues from patients with ICP and in human trophoblasts treated with hydrophobic bile acids, including chenodeoxycholic acid and deoxycholic acid. Furthermore, we found that treatment with hydrophobic bile acids impaired autophagic flux in both time- and concentration-dependent manners, by suppressing the AMP-activated protein kinase/unc-51-like kinase 1 autophagic signaling pathway. Notably, trophoblasts were prone to apoptotic cell death upon starvation along with bile-acids treatment in vitro or in an ICP mouse model in vivo. Additionally, we revealed mitochondrial dysfunction was the predominant biological process in excessive bile acids induced trophoblast impairment under starvation by proteomic assay. Collectively, our study proposed a complex interaction of excessive bile acids induced autophagic flux, mitochondrial dysfunction, and cellular apoptosis in placental trophoblasts may play a critical role in the pathogenesis of ICP.
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http://dx.doi.org/10.1002/jcp.30774DOI Listing
May 2022

Long non-coding RNAs and cancer mechanisms: Immune cells and inflammatory cytokines in the tumor microenvironment.

Med Oncol 2022 May 17;39(7):108. Epub 2022 May 17.

Department of Oncology, Second Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, People's Republic of China.

Chronic inflammation and immune response are two central hallmarks of the tumor microenvironment (TME), teeming with immune cells and inflammatory cytokines that promote tumor progression. Intriguingly, there is mutual regulation between immune cells and cytokines. Indeed, the differentiation and function of immune cells depend on cytokines secreted from tumor cells, whereas immune activation affects the dynamics of cytokines, reshaping the TME together. Long non-coding RNAs (lncRNAs) as a blooming molecule are virtually involved in physiology and pathology events, especially TME. Notably, the regulatory loop between lncRNAs and cytokines or immune activation plays a vital role in tumor growth. Thus, this review concentrates on the interaction between lncRNAs and immune cells. It puts special attention to the intertwist between lncRNAs and cytokines or immune cells, providing a theoretical basis for lncRNAs as a potential biomarker and therapeutic tumor target.
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http://dx.doi.org/10.1007/s12032-022-01680-5DOI Listing
May 2022

Multi-Element Analysis and Origin Discrimination of Based on Inductively Coupled Plasma Tandem Mass Spectrometry (ICP-MS/MS).

Molecules 2022 May 6;27(9). Epub 2022 May 6.

State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, National Engineering Research Center for Applied Technology of Agricultural Biodiversity, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China.

is an important functional health product, and has been used worldwide because of a wide range of pharmacological activities, of which the taproot is the main edible or medicinal part. However, the technologies for origin discrimination still need to be further studied. In this study, an ICP-MS/MS method for the accurate determination of 49 elements was established, whereby the instrumental detection limits (LODs) were between 0.0003 and 7.716 mg/kg, whereas the quantification limits (LOQs) were between 0.0011 and 25.7202 mg/kg, recovery of the method was in the range of 85.82% to 104.98%, and the relative standard deviations (RSDs) were lower than 10%. Based on the content of multi-element in (total of 89 mixed samples), the discriminant models of origins and cultivation models were accurately determined by the neural networks (prediction accuracy was 0.9259 and area under ROC curve was 0.9750) and the support vector machine algorithm (both 1.0000), respectively. The discriminant models established in this study could be used to support transparency and traceability of supply chains of and thus avoid the fraud of geographic identification.
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http://dx.doi.org/10.3390/molecules27092982DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9105934PMC
May 2022

Perineural invasion is a prognostic factor in stage II colorectal cancer but not a treatment indicator for traditional chemotherapy: a retrospective cohort study.

J Gastrointest Oncol 2022 Apr;13(2):710-721

Department of Colorectal Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Background: Perineural invasion (PNI) is considered a risk factor of survival but does not yet inform treatment decisions, and has not been studied separately in stage II colorectal cancer (CRC) patients whose postoperative traditional chemotherapy is controversial. This cohort study aimed to assess the association of PNI with basic clinicopathological features and patient outcomes after curative resection and the effects of PNI on responses to adjuvant chemotherapy in stage II CRC.

Methods: The clinical data of 371 stage II CRC patients who underwent curative-intent surgery at the National Cancer Center/Cancer Hospital in 2014 were retrospectively reviewed. The adjuvant chemotherapy data were acquired from follow-up information. PNI status was examined, and the overall survival (OS) and disease-free survival (DFS) rates were analyzed.

Results: PNI was detected in 82 of the 371 patients (22.1%) and was closely correlated with preoperative serum carcinoembryonic antigen (CEA) levels (P=0.030), gross tumor type (P=0.010), tumor differentiation (P=0.010), p stage (P<0.001), and extramural vascular invasion (EMVI) (P<0.001). The median follow-up time was 71 months. The 5-year OS was 84.1% and 96.5% (P<0.001), and the 5-year DFS was 75.6% and 91.3% (P<0.001) for PNI-positive (+) and PNI-negative (-) patients, respectively. The multivariate regression analyses identified PNI as an independent negative prognostic factor for DFS [hazard ratio (HR): 2.95; 95% confidence interval (CI), 1.546-5.626; P=0.001] and OS (HR: 3.966; 95% CI, 1.642-9.575; P=0.002). Among PNI (+) patients, DFS and OS were positively correlated with CEA levels (P=0.005 and P=0.004, respectively). Postoperative chemotherapy failed to improve DFS (P=0.480 and P=0.267, respectively) and OS (P=0.940 and P=0.077, respectively) regardless of whether the patients were PNI positive or not.

Conclusions: In stage II CRC patients, PNI was a poor independent predictor for DFS and OS. Among PNI (+) patients, CEA levels were positively correlated with DFS and OS. Traditional postoperative adjuvant chemotherapy does not improve outcomes of PNI (+) patients. Therefore, as to the active role of PNI and vacancy for treatment in allusion to PNI, follow-up of PNI (+) patients with elevated CEA level should be strengthened and further research on drug conducted on PNI deserve to be carried on.
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http://dx.doi.org/10.21037/jgo-22-277DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9086063PMC
April 2022

Single and Combined Effects of and Coccidiosis Vaccine on Growth Performance and the Intestinal Microbiome of Broiler Chickens.

Front Microbiol 2022 25;13:811428. Epub 2022 Apr 25.

Zhaoqing/Maoming Branch Center of Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Key Laboratory of Avian Influenza and Other Major Poultry Diseases Prevention and Control, Ministry of Agriculture and Rural Affairs, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, China.

Avian coccidiosis is an important intestinal protozoan disease that has caused major economic losses to the poultry industry. can not only maintain the stability of the intestinal barrier, but can also improve the production performance of broiler chickens. We studied the effects of feeding alone, administration of coccidiosis vaccine alone, and the combined administration of and coccidiosis vaccine on body weight gain, feed consumption, and feed conversion ratio of broilers. Meanwhile, intestinal contents of 8- and 15-day-old broilers were collected, and their intestinal microbiome was characterized by high-throughput sequencing of the V3-V4 region of 16S rDNA. We analyzed the oocysts per gram values and lesion scores in the alone group, in a group challenged with the coccidiosis-causing parasite, , and in groups simultaneously challenged and pretreated with , the coccidiosis vaccine, or combined and coccidiosis vaccine. Intestinal tissue samples were collected from 32-day-old broilers for microbiome analysis. Our results showed that combination of with coccidiosis vaccine significantly improved the performance of broiler chickens and also significantly reduced the oocysts per gram value and intestinal lesions caused by sp. infection. Furthermore, and coccidiosis vaccine administered alone or in combination significantly increased the relative abundance of the immune biomarker genus . The significant increase in the abundance of the Clostridia_UCG.014, group and was a key factor in controlling sp. infection.
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http://dx.doi.org/10.3389/fmicb.2022.811428DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9083122PMC
April 2022

Rapid qualitative and quantitative detection of Salmonella typhimurium using a single-step dual photometric/fluorometric assay.

Mikrochim Acta 2022 05 11;189(6):218. Epub 2022 May 11.

School of Public Health, Jilin University, Changchun, 130021, People's Republic of China.

A dual-signal photometric/fluorometric assay was established for rapid, qualitative, and quantitative detection of Salmonella typhimurium (S. typhimurium). This method was composed of two parts: (1) a single-step photometric (SSC) assay containing gold nanoparticles (AuNPs), poly-diallyldimethylammonium chloride (PDDA), and S. typhimurium-specific aptamer, and (2) a fluorescence (FL) assay containing carboxyl-modified CdSe/ZnS quantum dots (QDs-). Users just need to drop samples contaminated with S. typhimurium into SSC assay; the apparent color change from red to blue can be observed in a short time (20 min). A smartphone app was developed to read the semiquantitative result. By subsequently adding one drop of FL assay into the reaction mixture, the generated fluorescence intensity reflected the concentration of S. typhimurium. The naked eye limit of detection (LOD) and fluorescent LOD were 10 cfu/mL and 10 cfu/mL, respectively. This method exhibited good selectivity. The reliability and practicability were verified by testing contaminated food, drinking water, and pets' urine.
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http://dx.doi.org/10.1007/s00604-022-05312-7DOI Listing
May 2022

Oligonucleotide Discrimination Enabled by Tannic Acid-Coordinated Film-Coated Solid-State Nanopores.

Langmuir 2022 May 11;38(20):6443-6453. Epub 2022 May 11.

State Key Lab for Mesoscopic Physics and Frontiers Science Center for Nano-Optoelectronics, School of Physics, Peking University, Beijing 100871, China.

Discrimination of nucleotides serves as the basis for DNA sequencing using solid-state nanopores. However, the translocation of DNA is usually too fast to be detected, not to mention nucleotide discrimination. Here, we utilized polyphenolic TA and Fe, an attractive metal-organic thin film, and achieved a fast and robust surface coating for silicon nitride nanopores. The hydrophilic coating layer can greatly reduce the low-frequency noise of an original unstable nanopore, and the nanopore size can be finely tuned at the nanoscale by simply adjusting the relative ratio of Fe and TA monomers. Moreover, the hydrogen bonding interaction formed between the hydroxyl groups provided by TA and the phosphate groups of DNAs significantly increases the residence time of a short double-strand (100 bp) DNA. More importantly, we take advantage of the different strengths of hydrogen bonding interactions between the hydroxyl groups provided by TA and the analytes to discriminate between two oligonucleotide samples (oligodeoxycytidine and oligodeoxyadenosine) with similar sizes and lengths, of which the current signal patterns are significantly different using the coated nanopore. The results shed light on expanding the biochemical functionality of surface coatings on solid-state nanopores for future biomedical applications.
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http://dx.doi.org/10.1021/acs.langmuir.2c00638DOI Listing
May 2022

QTL Analysis of Z414, a Chromosome Segment Substitution Line with Short, Wide Grains, and Substitution Mapping of qGL11 in Rice.

Rice (N Y) 2022 May 9;15(1):25. Epub 2022 May 9.

Rice Research Institute, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.

Most agronomic traits of rice (Oryza sativa), such as grain length, are complex traits controlled by multiple genes. Chromosome segment substitution lines (CSSLs) are ideal materials for dissecting these complex traits. We developed the novel rice CSSL 'Z414', which has short, wide grains, from progeny of the recipient parent 'Xihui 18' (an indica restorer line) and the donor parent 'Huhan 3' (a japonica cultivar). Z414 contains four substitution segments with an average length of 3.04 Mb. Z414 displays seven traits that significantly differ from those of Xihui 18, including differences in grain length, width, and weight; degree of chalkiness; and brown rice rate. We identified seven quantitative trait loci (QTL) that are responsible for these differences in an F population from a cross between Xihui 18 and Z414. Among these, six QTL (qPL3, qGW5, qGL11, qRLW5, qRLW11, and qGWT5) were detected in newly developed single-segment substitution lines (SSSLs) S1-S6. In addition, four QTL (qGL3, qGL5, qCD3, and qCD5) were detected in S1 and S5. Analysis of these SSSLs attributed the short, wide grain trait of Z414 to qGL11, qGL3, qGL5, and qGW5. Substitution mapping delimited qGL11 within an 810-kb interval on chromosome 11. Sequencing, real time quantitative PCR, and cell morphology analysis revealed that qGL11 might be a novel QTL encoding the cyclin CycT1;3. Finally, pyramiding qGL3 (a = 0.43) and qGL11 (a =  - 0.37) led to shorter grains in the dual-segment substitution line D2 and revealed that qGL11 is epistatic to qGL3. In addition, S1 and D2 exhibited different grain sizes and less chalkiness than Z414. In conclusion, the short grain phenotype of the CSSL Z414 is controlled by qGL11, qGL3, and qGL5. qGL11 might be a novel QTL encoding CycT1;3, whose specific role in regulating grain length was previously unknown, and qGL11 is epistatic to qGL3. S1 and D2 could potentially be used in hybrid rice breeding.
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http://dx.doi.org/10.1186/s12284-022-00571-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9085999PMC
May 2022

Case Report: F-PSMA-1007 PET/CT Avid Solitary Penile Metastasis of Castration-Resistant Prostate Cancer With a PSA of 0.072 ng/ml.

Front Oncol 2022 20;12:881896. Epub 2022 Apr 20.

Department of Nuclear Medicine, General Hospital of Ningxia Medical University, Yinchuan, China.

Penile metastasis of prostate cancer is rare, with a poor prognosis, and only a limited number of relevant cases have been reported so far. With the application of F-PSMA-1007 PET/CT, the biochemical recurrence of prostate cancer can be detected at an early stage for providing important evidence, facilitating clinical decision-making. Here, we have reported a case of solitary penile metastatic recurrence in the context of mild PSA progression (PSA: 0.072 ng/ml). This case highlights the preferable sensitivity of F-PSMA-1007 PET/CT imaging in prostate cancer.
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http://dx.doi.org/10.3389/fonc.2022.881896DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9067612PMC
April 2022

Efficacy and safety of dexmedetomidine-ropivacaine versus sufentanil-ropivacaine for epidural labor analgesia: a randomized controlled trial.

Ann Palliat Med 2022 Apr;11(4):1410-1420

Department of Anesthesiology, First Affiliated Hospital of Army Medical University. Chongqing, China.

Background: Sufentanil combined with ropivacaine is commonly used for epidural labor analgesia, but it may cause some adverse effects. Dexmedetomidine is suitable for long-term and short-term intraoperative sedation and analgesia, and it can reduce the use of the opioid sufentanil. This study compared the efficacy and safety of dexmedetomidine and sufentanil combined with ropivacaine for epidural labor analgesia.

Methods: A randomized, triple-blinded, controlled trial was performed for epidural labor analgesia. All included parturient women were randomized 1:1 by computer to receive ropivacaine combined with dexmedetomidine (group RD) or sufentanil (group RS) from October 2020 to February 2021. The primary outcomes were the pain relief of parturient women assessed by the visual analog scale (VAS) score, and the physical status of newborns assessed by the neonatal behavioral neurological assessment (NBNA) score and Apgar score. Secondary outcomes included the duration of labor stages and adverse reactions of parturient women and newborns.

Results: A total of 160 parturient women were included in this study, with 80 in each group. The VAS scores were lower in both groups after the injection of analgesics (in 120 min; RD: 2.6±1.0 vs. RS: 2.5±0.8; P=0.489), and there was no significant difference. The newborns' NBNA (RD: 39.9±0.4 vs. RS: 39.8±0.5; P=0.368) and Apgar scores (RD: 9.8±0.7 vs. RS: 9.7±0.8; P=0.424) were higher than normal standard (NBNA >37; Apgar >7) in both groups, and there were also no significant differences. No differences in parturient women's demographic characteristics, vital signs, Ramsay Sedation Scale (RSS) values, blood loss, duration of labor stages (first and second stages), onset time of analgesia, and dose of analgesics were found between the 2 groups (all P>0.05). The incidence of adverse reactions in parturient women and newborns was low in both groups.

Conclusions: Dexmedetomidine or sufentanil combined with ropivacaine for epidural labor analgesia had similar analgesic effects in clinical practice.

Trial Registration: Chinese Clinical Trial Registry ChiCTR2000038702.
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http://dx.doi.org/10.21037/apm-22-264DOI Listing
April 2022

Effect of coagulation on microfibers in laundry wastewater.

Environ Res 2022 May 3:113401. Epub 2022 May 3.

Chemical and Biochemical Engineering, University of Western Ontario, London, ON, N6A5B9, Canada. Electronic address:

Microplastics pollution in the aquatic system has received significant attention due to their recalcitrant nature and ecotoxicological threat. Municipal wastewater typically contains various microplastics with synthetic microfibers as a significant constituent from the laundry process. The fate of microfibers in conventional wastewater processes is not clearly understood. In this study, the effect of coagulation on microfibers obtained from a lint screen of a domestic dryer and resuspended in pure water, and also in laundry wastewater was investigated using ferric chloride and polyaluminum chloride (PACl). The removal efficiency of the microfibers resuspended in pure water varied from 86% to 96% depending on the fiber size ranges: < 90 μm, 90-125 μm, and >125 μm with the smaller size microfibers showing a lower removal efficiency. Surfactant present in detergent in laundry wastewater reduced the microfibers removal efficiency to 0-37%, however, the addition of PACl increased microfibers removal to 90%. The optimal PACl concentrations for ≥90% removal were 1.75, 2, 4, and 6 mg/L for 0.5, 2, 4, 8 mg/L detergent, respectively. Zeta potential, FTIR, and SEM analysis were applied to observe the surface changes of microfibers during coagulation indicating possible mechanisms of coagulation. The dominant mechanisms for coagulation of microfibers by FeCl and PACl seem to be charge neutralization and adsorption-bridging. This work provided some insights about the fate of laundry microfibers in primary treatment processes.
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http://dx.doi.org/10.1016/j.envres.2022.113401DOI Listing
May 2022

Ambient temperature exposure and risk of outpatient visits for dermatologic diseases in Xinxiang, China: a time-series analysis.

Int J Biometeorol 2022 May 6. Epub 2022 May 6.

Henan International Collaborative Laboratory for Health Effects and Intervention of Air Pollution, School of Public Health, Xinxiang Medical University, Xinxiang, 453003, Henan Province, China.

The effect of ambient temperature on dermatologic diseases has received widespread attention. Previous studies have shown that ambient temperature might affect specific dermatologic diseases, but results were inconsistent. This study aims to assess the short-term effect of ambient temperature on outpatient visits due to dermatologic diseases (DMs) in Xinxiang, China. Daily DMs outpatient visits, mean temperature, mean relative humidity, and air pollution data of Xinxiang were retrieved from January 1, 2015, to December 31, 2018. A distributed lag nonlinear model (DLNM) was applied to analyze the effect of ambient temperature on DMs outpatients. We controlled several potential confounding factors such as the long-term trend, public holiday, day of the week, humidity, and air pollutants (NO, PM). Finally, two more stratification analysis was conducted by age and gender. A total of 164,270 outpatients of DMs were enrolled during our study, and the daily mean visits were 113. The estimated effect of temperature on DMs was nonlinear. Heat temperature would exacerbate outpatients of dermatologic diseases. With a reference median temperature (17 °C), the effect of temperature on DMs was most pronounced at lag0-14; exposure to heat (32 °C, 99th) was associated with 1.565 (95% CI: 1.266-1.934) increased risk of outpatients for DMs. Stratification analysis showed that citizens of young ages were susceptive to heat; both genders had a similar relationship between temperature and DMs risk. This study highlights that ambient temperature was associated with DMs outpatients; heat temperature might aggravate DMs risk. The health hazards of heat temperature required more attention, and more effective prevention measurements should be designed and implemented to curb global warming.
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http://dx.doi.org/10.1007/s00484-022-02297-zDOI Listing
May 2022

Multitrophic diversity and biotic associations influence subalpine forest ecosystem multifunctionality.

Ecology 2022 May 6:e3745. Epub 2022 May 6.

CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China.

Biodiversity across multiple trophic levels is required to maintain multiple ecosystem functions. Yet, it remains unclear how multitrophic diversity and species interactions regulate ecosystem multifunctionality. Here, combining data from nine different trophic groups (including trees, shrubs, herbs, leaf mites, small mammals, bacteria, pathogenic fungi, saprophytic fungi and symbiotic fungi) and 13 ecosystem functions related to supporting, provisioning and regulating services, we used a multitrophic perspective to evaluate the effects of elevation, diversity and network complexity on scale-dependent subalpine forest multifunctionality. Our results demonstrate that elevation and soil pH significantly modified species composition and richness across multitrophic groups and influenced multiple functions simultaneously. We provide evidence that species richness across multiple trophic groups had stronger effects on multifunctionality than species richness at any single trophic level. Moreover, biotic associations, indicating the complexity of trophic networks, were positively associated with multifunctionality. The relative effects of diversity on multifunctionality increased at the scale of the larger community compared to a scale accounting for neighbouring interactions. Our results highlight the paramount importance of scale- and context- dependent multitrophic diversity and interactions for a better understanding of mountain ecosystem multifunctionality in a changing world. This article is protected by copyright. All rights reserved.
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http://dx.doi.org/10.1002/ecy.3745DOI Listing
May 2022

Clinical features of acute focal bacterial nephritis in adults.

Sci Rep 2022 May 4;12(1):7292. Epub 2022 May 4.

Department of Nephrology, The Second Hospital of Hebei Medical University, Shijiazhuang, China.

Acute focal bacterial nephritis (AFBN) is a localized bacterial infection of the kidneys presenting as an inflammatory mass that can develop into renal abscess. The current reports on AFBN mostly are among children and rarely described in adults. This study was aimed to analyze the clinical features of AFBN in adults and make a review for the disease to give the clinicians some clues to suspect and recognize it in adults. From January 2014 to December 2019, AFBN was diagnosed by contrast-enhanced computed tomography (CT) in 238 adults at the Department of Nephrology, the Second Hospital of Hebei Medical University, Shijiazhuang, China. We reviewed the clinical records of these patients and asked them about their post-discharge status via telephone follow-up. Of all the patients, 195 were female and 43 were male, the median age were 46.87 years. 86.13% presented with fever, 55.89% presented with lower urinary tract symptoms and 97.9% presented with pyuria. In renal ultrasonography, abdominal findings were seen only 22.69% patients. E.coli accounted for 74.73% of the isolated pathogen. After 4 weeks of treatment, the patients had no recurrence of symptoms. We recommend that when a patient presents clinically with acute pyelonephritis, but the fever persist longer after antimicrobial treatment (≥ 4 days in our study), AFBN should be suspected. For the diagnosis, contrast-enhanced CT is the "gold standard", magnetic resonance imaging (MRI) may be a good option, but the ultrasonography is probably not satisfied. 3-4 weeks of antibiotic therapy may be appropriate for AFBN in adults.
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http://dx.doi.org/10.1038/s41598-022-10809-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068887PMC
May 2022

Replacing liquid chromatography with tailored ion chromatography: A green method for detecting furfuryl alcohol and understanding its properties.

J Chromatogr A 2022 Apr 26;1673:463090. Epub 2022 Apr 26.

State Key Laboratory of Urban Water Resource and Environment, Shenzhen Key Laboratory of Organic Pollution Prevention and Control, Harbin Institute of Technology, Shenzhen 518055, China.

Furfuryl alcohol (FFA) is a furan derivative potentially hazardous to human health, and it is now ubiquitously found in foods and used for identifying singlet oxygen (O) in environmental studies. However, current analytical methods for FFA in water are mostly based on gas chromatography and liquid chromatography (LC), which inevitably employ organic solvents as extractants or eluents that can result in harmful wastes production and extra treatment costs. To solve this issue, we herein developed a green analytical method to measure FFA without using organic solvents by a tailored ion chromatography (IC) equipped with ultraviolet (UV) detector. The method demonstrated a calibration curve fitting well (R > 0.99) for a wide FFA concentration range (i.e., 0.1 ∼ 10.0 mg/L) and a method detection limit (0.031 mg/L) comparable to LC method. The recoveries of FFA dosed into real samples exceeded 86.4% with the relative standard deviations below 2.5%. Next, we examined the property and reactivity of FFA through the method. It was found that FFA's acid-dissociation coefficient (i.e., pK) was not 9.55, which disagrees with an earlier report. FFA was resistant to 254 nm UV or hydrogen peroxide (HO) but vulnerable to HO-assisted 254 nm UV or 185 nm vacuum UV, confirming that FFA was sensitive to hydroxyl radicals. Interestingly, FFA was degraded to less extent in water dosed with both sodium hypochlorite (NaOCl) and HO than that dosed with NaOCl only, suggesting that the reaction between NaOCl and HO does not produce O. Given that this IC method can analyze FFA, NaOCl, and HO simultaneously in one run, the evidences presented here may have helped clear a controversy about the O formation possibility by NaOCl and HO.
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http://dx.doi.org/10.1016/j.chroma.2022.463090DOI Listing
April 2022

HO-independent chemodynamic therapy initiated from magnetic iron carbide nanoparticle-assisted artemisinin synergy.

RSC Adv 2021 Nov 22;11(59):37504-37513. Epub 2021 Nov 22.

Clinical Research Institute, Zhejiang Provincial People's Hospital Hangzhou 310014 China

Chemodynamic therapy (CDT) is a booming technology that utilizes Fenton reagents to kill tumor cells by transforming intracellular HO into reactive oxygen species (ROS), but insufficient endogenous HO makes it difficult to attain satisfactory antitumor results. In this article, a HO-free CDT technique with tumor-specificity is developed by using pH-sensitive magnetic iron carbide nanoparticles (PEG/[email protected] NPs) to trigger artemisinin (ART) to form ROS. ART-loaded PEG/[email protected] NPs are fabricated for the enormous release of Fe ions induced by the acidic conditions of the tumor microenvironment after magnetic-assisted tumor enrichment, which results in the rapid degradation of the PEG/[email protected] NPs and release of ART once endocytosed into tumor cells. catalysis reaction between the co-released Fe ions and ART generates toxic ROS and then induces apoptosis of tumor cells. Both and experiments demonstrate that the efficient Fe-enhanced and tumor-specific CDT efficacy for effective tumor inhibition based on ROS generation. This work provides a new direction to improve CDT efficacy based on HO-independent ROS generation.
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http://dx.doi.org/10.1039/d1ra04975eDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043768PMC
November 2021

Rapid detection of using magnetic nanobead-based immunoseparation and quantum dot-based immunofluorescence.

RSC Adv 2021 Nov 1;11(61):38638-38647. Epub 2021 Dec 1.

Department of Hygienic Inspection, School of Public Health, Jilin University 1163 Xinmin Street Changchun 130021 Jilin China +86 43185619441.

In recent years, the scale of population exposure and food poisoning caused by () has shown a significant upward trend, becoming one of the primary food-borne pathogens. Herein, we developed a rapid and sensitive detection of by integrating the technology of magnetic nanobeads (MBs) based immunoseparation (IMS) with quantum dots (QDs) based immunofluorescence. Firstly, specific rabbit polyclone IgG antibodies (IgG) and chicken egg yolk antibodies (IgY) of were prepared. Then two sizes of MBs (1 μm; 180 nm) were coupled with IgG to form immuno-MB (IMB) capture probes for evaluating the effect of different sizes on the detection efficiency. For QDs, they were conjugated with IgY to form fluorescent reporting probes. In the process of detection, IMB probes were used to separate and then these complexes were labeled by QD probes on the principle of double antibody sandwich. The fluorescence intensity of the IMB--QD complexes was measured by a fluorescence spectrophotometer. The detection method takes 150 min with a detection limit of 10 cfu mL ranging from 10 to 10 cfu mL and it has been shown to work satisfactorily in real food samples.
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http://dx.doi.org/10.1039/d1ra07580bDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044181PMC
November 2021
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