Publications by authors named "Juan Cui"

92 Publications

Meta-analysis of the diagnostic value of serum, plasma and urine neutrophil gelatinase-associated lipocalin for the detection of acute kidney injury in patients with sepsis.

Exp Ther Med 2021 Apr 23;21(4):386. Epub 2021 Feb 23.

Department of Cardiac Surgery, Peking Union Medical College Hospital, Beijing 100730, P.R. China.

The objective of the present study was to assess the diagnostic value of urine, serum and plasma neutrophil gelatinase-associated lipocalin (NGAL) for the early diagnosis of acute kidney injury (AKI) among patients with suspected sepsis. Therefore, a meta-analysis was carried out to evaluate diagnostic accuracy data from the literature regarding the diagnosis of AKI in patients with sepsis. Electronic databases were systematically searched for relevant studies and quality assessment was conducted using the Quality Assessment for Diagnostic Accuracy Studies 2 tool. A summary receiver operating characteristic curve analysis was performed, and several parameters including sensitivity, specificity, diagnosis odds ratio (DOR) and area under the curve (AUC) were calculated to evaluate the diagnostic performance of urine, serum and plasma NGAL. Meta-regression, sensitivity and subgroup analysis were also conducted to identify the source of heterogeneity in the eligible studies. In total, 28 studies were included. The pooled sensitivities for urine, serum and plasma NGAL were 0.87, 0.83 and 0.80, respectively. Pooled specificity was 0.84, 0.79 and 0.74. The DORs were 35, 18 and 11, respectively. The AUC for urine, serum and plasma NGAL were 0.92, 0.87 and 0.84, respectively. Urine NGAL presented superior performance for the diagnosis of AKI with the highest AUC and other diagnostic accuracy values, compared with serum and plasma NGAL. Further studies are needed to clarify the controversial issue between the usefulness of serum and plasma NGAL.
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http://dx.doi.org/10.3892/etm.2021.9817DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7918111PMC
April 2021

Identification of anti-inflammatory vesicle-like nanoparticles in honey.

J Extracell Vesicles 2021 Feb 12;10(4):e12069. Epub 2021 Feb 12.

Department of Nutrition and Health Sciences University of Nebraska-Lincoln Lincoln Nebraska USA.

Honey has been used as a nutrient, an ointment, and a medicine worldwide for many centuries. Modern research has demonstrated that honey has many medicinal properties, reflected in its anti-microbial, anti-oxidant, and anti-inflammatory bioactivities. Honey is composed of sugars, water and a myriad of minor components, including minerals, vitamins, proteins and polyphenols. Here, we report a new bioactive component‒vesicle-like nanoparticles‒in honey (H-VLNs). These H-VLNs are membrane-bound nano-scale particles that contain lipids, proteins and small-sized RNAs. The presence of plant-originated plasma transmembrane proteins and plasma membrane-associated proteins suggests the potential vesicle-like nature of these particles. H-VLNs impede the formation and activation of the nucleotide-binding domain and leucine-rich repeat related (NLR) family, pyrin domain containing 3 (NLRP3) inflammasome, which is a crucial inflammatory signalling platform in the innate immune system. Intraperitoneal administration of H-VLNs in mice alleviates inflammation and liver damage in the experimentally induced acute liver injury. miR-4057 in H-VLNs was identified in inhibiting NLRP3 inflammasome activation. Together, our studies have identified anti-inflammatory VLNs as a new bioactive agent in honey.
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http://dx.doi.org/10.1002/jev2.12069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7879699PMC
February 2021

High-entropy-stabilized chalcogenides with high thermoelectric performance.

Science 2021 02;371(6531):830-834

Shenzhen Key Laboratory of Thermoelectric Materials, Department of Physics, Southern University of Science and Technology, Shenzhen 518055, China.

Thermoelectric technology generates electricity from waste heat, but one bottleneck for wider use is the performance of thermoelectric materials. Manipulating the configurational entropy of a material by introducing different atomic species can tune phase composition and extend the performance optimization space. We enhanced the figure of merit () value to 1.8 at 900 kelvin in an n-type PbSe-based high-entropy material formed by entropy-driven structural stabilization. The largely distorted lattices in this high-entropy system caused unusual shear strains, which provided strong phonon scattering to largely lower lattice thermal conductivity. The thermoelectric conversion efficiency was 12.3% at temperature difference Δ = 507 kelvin, for the fabricated segmented module based on this n-type high-entropy material. Our demonstration provides a paradigm to improve thermoelectric performance for high-entropy thermoelectric materials through entropy engineering.
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http://dx.doi.org/10.1126/science.abe1292DOI Listing
February 2021

Elucidation of molecular links between obesity and cancer through microRNA regulation.

BMC Med Genomics 2020 10 30;13(1):161. Epub 2020 Oct 30.

Systems Biology and Biomedical Informatics Lab, Department of Computer Science and Engineering University of Nebraska-Lincoln, Lincoln, 68588-0115, NE, USA.

Background: Obesity contributes to high cancer risk in humans and the mechanistic links between these two pathologies are not yet understood. Recent emerging evidence has associated obesity and cancer with metabolic abnormalities and inflammation where microRNA regulation has a strong implication.

Methods: In this study, we have developed an integrated framework to unravel obesity-cancer linkage from a microRNA regulation perspective. Different from traditional means of identifying static microRNA targets based on sequence and structure properties, our approach focused on the discovery of context-dependent microRNA-mRNA interactions that are potentially associated with disease progression via large-scale genomic analysis. Specifically, a meta-regression analysis and the integration of multi-omics information from obesity and cancers were presented to investigate the microRNA regulation in a dynamic and systematic manner.

Results: Our analysis has identified a total number of 2,143 unique microRNA-gene interactions in obesity and seven types of cancer. Common interactions in obesity and obesity-associated cancers are found to regulate genes in key metabolic processes such as fatty acid and arachidonic acid metabolism and various signaling pathways related to cell growth and inflammation. Additionally, modulated co-regulations among microRNAs targeting the same functional processes were reflected through the analysis.

Conclusion: We demonstrated the statistical modeling of microRNA-mediated gene regulation can facilitate the association study between obesity and cancer. The entire framework provides a powerful tool to understand multifaceted gene regulation in complex human diseases that can be generalized in other biomedical applications.
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http://dx.doi.org/10.1186/s12920-020-00797-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7596939PMC
October 2020

Effects of Temperature on the Development and Fecundity of Atractomorpha Sinensis (Orthoptera: Pyrgomorphidae).

J Econ Entomol 2020 10;113(5):2530-2539

College of Plant Protection, Jilin Agricultural University, Changchun, PR China.

Over the recent years, Atractomorpha sinensis I. Bolivar, has emerged as an important agricultural pest in China. However, the biological characteristics of A. sinensis remain largely unknown, which can hinder the prediction of its population dynamics. Thus, understanding the impact of temperature on the developmental period of A. sinensis is crucial to predict its population dynamics. The biological characteristics of A. sinensis were systematically observed at five different temperatures (16, 20, 24, 28, and 32°C) using the age-stage, two-sex life table method. The results demonstrated that the developmental period, preadult time, adult longevity, adult preoviposition period, and total preoviposition period were significantly reduced when the temperature was elevated from 16 to 32°C. The developmental threshold temperatures of egg, nymph, preoviposition period, female adult, male adult, and generation were 9.14, 10.44, 12.53, 10.97, 12.47, and 10.58°C, respectively, with the corresponding effective accumulated temperatures of 452.31, 575.99, 169.58, 528.13, 340.81, and 1447.95 degree-days. With an increase in temperature, the intrinsic rate of increase (r) and finite rate of increase (λ) were increased, while the mean generation time (T) was shortened. The optimal values of net reproductive rate (R0= 73.00 offspring) and fecundity (244.55 eggs) were determined at 24°C. Similarly, the population trend index (I) of A. sinensis was found to be highest at 24°C. Our findings indicate that A. sinensis has the greatest rate of population growth at 24°C, which can provide a scientific basis for predicting the in-field population dynamics of A. sinensis.
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http://dx.doi.org/10.1093/jee/toaa145DOI Listing
October 2020

Milk exosomes and miRNA cross the placenta and promote embryo survival in mice.

Reproduction 2020 10;160(4):501-509

Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.

Exosomes facilitate cell-to-cell communication by transferring regulatory molecules such as miRNA from donor to recipient cells, for example, miR-21-5p and miR-30d promote placentation. Exosomes and their miRNA cargos are not exclusively obtained from endogenous synthesis but may also be absorbed from dietary sources, such as milk. This study assessed the effects of milk exosomes and miRNA cargos on embryo development and fertility in C57BL/6 mice. Fluorophore-labeled milk exosomes, miR-21-5p and miR-30d accumulated in murine placenta and embryos following oral gavage. Seventeen mRNAs, miR-21-5p and miR-30d were differentially expressed in placentas of pregnant mice fed a milk exosome and RNA-depleted (ERD) diet or a milk exosome and RNA-sufficient (ERS) diet. Eight of these mRNAs encode proteins implicated in the synthesis of extracellular matrix components, cell adhesion and migration. Changes in mRNA expression were associated with corresponding changes in protein expression, for example, collagen type I. The size of litters born to dams fed ERD was 25-50% smaller than those born to ERS controls. This study implicates dietary exosomes and miRNA in placenta development and embryo survival.
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http://dx.doi.org/10.1530/REP-19-0521DOI Listing
October 2020

Permeable hollow 3D tissue-like constructs engineered by on-chip hydrodynamic-driven assembly of multicellular hierarchical micromodules.

Acta Biomater 2020 09 11;113:328-338. Epub 2020 Jun 11.

Key Laboratory of Biomimetic Robots and Systems (Beijing Institute of Technology), Ministry of Education, Beijing, 100081, China.

Engineered three-dimensional (3D) microtissues that recapitulate in vivo tissue morphology and microvessel lumens have shown significant potential in drug screening and regenerative medicine. Although microfluidic-based techniques have been developed for bottom-up assembly of 3D tissue models, the spatial organization of heterogeneous micromodules into tissue-specific 3D constructs with embedded microvessels remains challenging. Inspired by a hydrodynamic-based classic game which stacks rings in water through the flow, a facile strategy is proposed for effective assembly of heterogeneous hierarchical micromodules with a central hole, into permeable hollow 3D tissue-like constructs through hydrodynamic interaction in a versatile microfluidic chip. The micromodules are fabricated by in situ multi-step photo-crosslinking of cell-laden hydrogels with different mechanical properties to give the high fidelity. With the hydrodynamic interaction derived from the discontinuous circulating flow, the micromodules are spatially organized layer-by-layer to form a 3D construct with a microvessel-like lumen. As an example, a ten-layered liver lobule-like construct containing inner radial-like poly(ethylene glycol) diacrylate (PEGDA) structure with hepatocytes and outer hexagonal gelatin methacrylate (GelMA) structure with endothelial cells are assembled in 2 min. During 10 days of co-culture, cells maintain high viability and proliferated along with the composite lobule-like morphology. The 3D construct owns a central lumen, which allows perfusion culture to promote albumin secretion. We anticipate that this microassembly strategy can be used to fabricate vascularized 3D tissues with various physiological morphologies as alternatives for biomedical research applications. STATEMENT OF SIGNIFICANCE: Microfluidic-based assembly is an attractive approach for the fabrication of 3D tissue models using cell-laden hydrogel microstructures with single mechanical stability. However, native tissues are complex 3D structures with indispensable vessels and multiple mechanical properties, which is still challenging to recreate. This study proposed a novel strategy to fabricate tissue-like 3D constructs with embedded lumen through hydrodynamic interaction using multicellular micromodules with hierarchical mechanical properties. The resultant hollow 3D constructs allow perfusion co-culture to enhance cell activity. This strategy relies on a simple and facile microfluidic chip to fabricate various 3D tissue-like constructs with hierarchical mechanical properties and permeable lumen, which can potentially be used as in vitro perfusion models for biomedical research.
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http://dx.doi.org/10.1016/j.actbio.2020.06.010DOI Listing
September 2020

Associations between air pollution and outpatient visits for allergic rhinitis in Xinxiang, China.

Environ Sci Pollut Res Int 2020 Jul 15;27(19):23565-23574. Epub 2020 Apr 15.

School of Public Health, Xinxiang Medical University, Xinxiang, 453003, China.

Several epidemiological studies have investigated the adverse health effects of air pollution, but studies reporting its effects on allergic rhinitis (AR) are limited, especially in developing countries having the most severe pollution. Limited studies have been conducted in China, but their results were inconsistent. So, we conducted a time-series study to evaluate the acute effect of six air pollutants (fine particulate matter [PM], particulate matter with diameter less than 10 μm [PM], sulfur dioxide [SO], nitrogen dioxide [NO], ozone [O], and carbon monoxide [CO]) on hospital outpatient visits for AR in Xinxiang, China from January 1, 2015, to December 31, 2018. An over-dispersed Poisson generalized additive model adjusting for weather conditions, long-term trends, and day of the week was used. In total, 14,965 AR outpatient records were collected during the study period. Results found that each 10 μg/m increase in PM, PM, SO, NO, O, and CO corresponded to 0.70% (95% confidence interval 0.00-1.41%), 0.79% (0.35-1.23%), 3.43% (1.47-5.39%), 4.54% (3.01-6.08%), 0.97% (- 0.11-2.05%), and 0.07% (0.02-0.12%) increments in AR outpatients on the current day, respectively. In the stratification analyses, statistically stronger associations were observed with PM, PM, SO, NO, and CO for AR outpatients < 15 years of age than in those 15-65 and ≥ 65 years of age, whereas the opposite result was found with O. Associations between PM, SO, NO, O, and AR outpatients were higher in the warm season than those in the cool season. This study suggests that exposure to PM, PM, SO, NO, and CO was associated with increased AR risk and children younger than 15 years might be more vulnerable.
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http://dx.doi.org/10.1007/s11356-020-08709-0DOI Listing
July 2020

Effects of extracts from Chuanwu (Aconitum Carmichaelii) and Banxia (Rhizoma Pinelliae) on excisional wound healing in a rat's model.

J Tradit Chin Med 2019 02;39(1):65-73

Basic Medicine College, Nanyang Medical University, Nanyang 473061, China.

Objevtive: To investigate the effects of extracts from Chuanwu (Aconitum Carmichaelii) and Banxia (Rhizoma Pinelliae) on the excisional wound healing in a rat's model.

Methods: Rats were performed a surgical lesion with a 2.0 cm resected tissue in the dorsal fascia. Following, animals were randomly divided into model group, YNB group and APE group those were respectively treated with saline, 1 mg/mL of Yunnan Baiyao and 1 mg/mL of Chuanwu (Aconitum Carmichaelii) and Banxia (Rhizoma Pinelliae) extracts. Wound contractions in days 0, 3, 7, 14 and 21 were calculated by an image analyzer. Histological analysis was analyzed using hematoxilin and eosin. Levels of tumor necrosis factor α (TNF-α), interleukin-2 (IL-2), IL-4, IL-10, transforming growth factor-β1 (TGF-β1) and basic fibroblast growth factor (bFGF) were determined by real-time quantitative PCR.

Results: Compared with that of YNB group and APE gtoup, the skin of rats showed poor re-modeling and re-epithelization characterized by a significant decrease of neovascularization, epithelialization and fibroblast in the model group. In the APE group, levels of TNF-α and IL-2 were significantly down-regulated and IL-4 and IL-10 significantly up-regulated in contrasted with that of model group. In addition, levels of TGF-β1 and bFGF in the APE group were significantly induced compared with that of model group.

Conclusion: The results suggest that the extracts from Chuanwu (Aconitum Carmichaelii) and Banxia (Rhizoma Pinelliae) promote wound healing in the rats, which is associated with enhancing anti-inflammatory ability and inducing fibroblast formation.
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February 2019

Dietary Depletion of Milk Exosomes and Their MicroRNA Cargos Elicits a Depletion of miR-200a-3p and Elevated Intestinal Inflammation and Chemokine (C-X-C Motif) Ligand 9 Expression in Mice.

Curr Dev Nutr 2019 Dec 31;3(12):nzz122. Epub 2019 Oct 31.

Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE, USA.

Background: Exosomes transfer regulatory microRNAs (miRs) from donor cells to recipient cells. Exosomes and miRs originate from both endogenous synthesis and dietary sources such as milk. miR-200a-3p is a negative regulator of the proinflammatory chemokine (C-X-C motif) ligand 9 (CXCL9). Male mice spontaneously develop clinical signs of inflammatory bowel disease (IBD).

Objectives: We assessed whether dietary depletion of exosomes and miRs alters the severity of IBD in mice owing to aberrant regulation of proinflammatory cytokines.

Methods: Starting at 5 wk of age, 16 male mice were fed either milk exosome- and RNA-sufficient (ERS) or milk exosome- and RNA-depleted (ERD) diets. The ERD diet is characterized by a near-complete depletion of miRs and a 60% loss of exosome bioavailability compared with ERS. Mice were killed when their weight loss exceeded 15% of peak body weight. Severity of IBD was assessed by histopathological evaluation of cecum. Serum cytokine and chemokine concentrations and mRNA and miR tissue expression were analyzed by multiplex ELISAs, RNA-sequencing analysis, and qRT-PCR, respectively.

Results: Stromal collapse, gland hyperplasia, and additive microscopic disease scores were (mean ± SD) 56.7% ± 23.3%, 23.5% ± 11.8%, and 29.6% ± 8.2% lower, respectively, in ceca of ERS mice than of ERD mice ( < 0.05). The serum concentration of CXCL9 was 35.0% ± 31.0% lower in ERS mice than in ERD mice ( < 0.05). Eighty-seven mRNAs were differentially expressed in the ceca from ERS and ERD mice; 16 of these mRNAs are implicated in immune function. The concentrations of 4 and 1 out of 5 miRs assessed (including miR-200a-3p) were ≤63% lower in livers and ceca, respectively, from ERD mice than from ERS mice.

Conclusions: Milk exosome and miR depletion exacerbates cecal inflammation in mice.
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http://dx.doi.org/10.1093/cdn/nzz122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053579PMC
December 2019

Human body-fluid proteome: quantitative profiling and computational prediction.

Brief Bioinform 2021 Jan;22(1):315-333

Department of Computer Science and Engineering in the University of Nebraska-Lincoln.

Empowered by the advancement of high-throughput bio technologies, recent research on body-fluid proteomes has led to the discoveries of numerous novel disease biomarkers and therapeutic drugs. In the meantime, a tremendous progress in disclosing the body-fluid proteomes was made, resulting in a collection of over 15 000 different proteins detected in major human body fluids. However, common challenges remain with current proteomics technologies about how to effectively handle the large variety of protein modifications in those fluids. To this end, computational effort utilizing statistical and machine-learning approaches has shown early successes in identifying biomarker proteins in specific human diseases. In this article, we first summarized the experimental progresses using a combination of conventional and high-throughput technologies, along with the major discoveries, and focused on current research status of 16 types of body-fluid proteins. Next, the emerging computational work on protein prediction based on support vector machine, ranking algorithm, and protein-protein interaction network were also surveyed, followed by algorithm and application discussion. At last, we discuss additional critical concerns about these topics and close the review by providing future perspectives especially toward the realization of clinical disease biomarker discovery.
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http://dx.doi.org/10.1093/bib/bbz160DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820883PMC
January 2021

Dietary bovine milk exosomes elicit changes in bacterial communities in C57BL/6 mice.

Am J Physiol Gastrointest Liver Physiol 2019 11 11;317(5):G618-G624. Epub 2019 Sep 11.

Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska.

Exosomes and exosome-like vesicles participate in cell-to-cell communication in animals, plant, and bacteria. Dietary exosomes in bovine milk are bioavailable in nonbovine species, but a fraction of milk exosomes reaches the large intestine. We hypothesized that milk exosomes alter the composition of the gut microbiome in mice. C57BL/6 mice were fed AIN-93G diets, defined by their content of bovine milk exosomes and RNA cargos: exosome/RNA-depleted (ERD) versus exosome/RNA-sufficient (ERS) diets. Feeding was initiated at age 3 wk, and cecum content was collected at ages 7, 15, and 47 wk. Microbial communities were identified by 16S gene sequencing. Milk exosomes altered bacterial communities in the murine cecum. The abundance of three phyla, seven families, and 52 operational taxonomic units (OTUs) was different in the ceca from mice fed ERD and ERS ( < 0.05). For example, at the phylum level, Tenericutes had more than threefold abundance in ERS mice at ages 15 and 47 wk compared with ERD mice ( < 0.05). At the family level, Verrucomicrobiaceae were much less abundant in ERS mice compared with ERD mice age 47 wk ( < 0.05). At the OTU level, four OTUs from the family of Lachnospiraceae were more than two times more abundant in ERS mice compared with ERD at age 7 and 47 wk ( < 0.05). We conclude that exosomes in bovine milk alter microbial communities in nonbovine species, suggesting that exosomes and their cargos participate in the crosstalk between bacterial and animal kingdoms. This is the first report that exosomes from bovine milk alter microbial communities in mice. This report suggests that the gut microbiome facilitates cell-to-cell communication by milk exosomes across species boundaries, and milk exosomes facilitate communication across animal and bacteria kingdoms.
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http://dx.doi.org/10.1152/ajpgi.00160.2019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879888PMC
November 2019

Circulating microRNA trafficking and regulation: computational principles and practice.

Authors:
Juan Cui Jiang Shu

Brief Bioinform 2020 07;21(4):1313-1326

Systems Biology and Biomedical Informatics Laboratory, Department of Computer Science and Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA.

Rapid advances in genomics discovery tools and a growing realization of microRNA's implication in intercellular communication have led to a proliferation of studies of circulating microRNA sorting and regulation across cells and different species. Although sometimes, reaching controversial scientific discoveries and conclusions, these studies have yielded new insights in the functional roles of circulating microRNA and a plethora of analytical methods and tools. Here, we consider this body of work in light of key computational principles underpinning discovery of circulating microRNAs in terms of their sorting and targeting, with the goal of providing practical guidance for applications that is focused on the design and analysis of circulating microRNAs and their context-dependent regulation. We survey a broad range of informatics methods and tools that are available to the researcher, discuss their key features, applications and various unsolved problems and close this review with prospects and broader implication of this field.
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http://dx.doi.org/10.1093/bib/bbz079DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7412956PMC
July 2020

Three-Dimensional Autofocusing Visual Feedback for Automated Rare Cells Sorting in Fluorescence Microscopy.

Micromachines (Basel) 2019 Aug 27;10(9). Epub 2019 Aug 27.

Beijing Advanced Innovation Center for Intelligent Robots and Systems, Beijing Institute of Technology, Beijing 100081, China.

Sorting rare cells from heterogeneous mixtures makes a significant contribution to biological research and medical treatment. However, the performances of traditional methods are limited due to the time-consuming preparation, poor purity, and recovery rate. In this paper, we proposed a cell screening method based on the automated microrobotic aspirate-and-place strategy under fluorescence microscopy. A fast autofocusing visual feedback (FAVF) method is introduced for precise and real-time three-dimensional (3D) location. In the context of this method, the scalable correlation coefficient (SCC) matching is presented for planar locating cells with regions of interest (ROI) created for autofocusing. When the overlap occurs, target cells are separated by a segmentation algorithm. To meet the shallow depth of field (DOF) limitation of the microscope, the improved multiple depth from defocus (MDFD) algorithm is used for depth detection, taking 850 ms a time with an accuracy rate of 96.79%. The neighborhood search based algorithm is applied for the tracking of the micropipette. Finally, experiments of screening NIH/3T3 (mouse embryonic fibroblast) cells verifies the feasibility and validity of this method with an average speed of 5 cells/min, 95% purity, and 80% recovery rate. Moreover, such versatile functions as cell counting and injection, for example, could be achieved by this expandable system.
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http://dx.doi.org/10.3390/mi10090567DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780806PMC
August 2019

Identification of age- and gender-associated long noncoding RNAs in the human brain with Alzheimer's disease.

Neurobiol Aging 2019 09 6;81:116-126. Epub 2019 Jun 6.

Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA. Electronic address:

Alzheimer's disease (AD) is an age- and gender-associated brain disorder. Long noncoding RNAs (lncRNAs) have emerged as key regulators of brain development, homeostasis, and pathologies. Here, we used gene array data sets and bioinformatics analysis to identify differentially expressed age- and gender-associated lncRNAs in human AD brains. We found that the expressions of 16 age-associated and 13 gender-associated lncRNAs were dysregulated in AD brains. Notably, the expressions of age-associated lncRNAs-SNHG19 and LINC00672-were significantly correlated with Braak stage of AD, positively and negatively, respectively, whereas the expressions of gender-associated lncRNAs-RNF144A-AS1, LY86-AS1, and LINC00639-were negatively correlated with Braak stage of AD. Functional analysis suggests that the pathways involved in neurodegenerative diseases, synaptic vesicle cycle, and endocytosis were overly represented within age- and gender-associated lncRNA-correlating genes. The identification of age- and gender-associated lncRNAs and their differential expressions in the human AD brain provide potential targets for further experimental validation and mechanistic investigation, which could, in turn, pave the way for developing age- and gender-specific prevention and adjunctive therapeutic options for patients with AD.
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http://dx.doi.org/10.1016/j.neurobiolaging.2019.05.023DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6732230PMC
September 2019

3D Construction of Shape-Controllable Tissues through Self-Bonding of Multicellular Microcapsules.

ACS Appl Mater Interfaces 2019 Jul 18;11(26):22950-22961. Epub 2019 Jun 18.

Intelligent Robotics Institute, School of Mechatronical Engineering , Beijing Institute of Technology , 5 South Zhongguancun Street , Haidian District, Beijing 100081 , China.

Designed microtissues that replicate highly ordered three-dimensional (3D) multicellular in vivo structures have shown huge potential in biomedical research and drug discovery. Through microencapsulation and microfluidic techniques, cell-laden microcapsules have been widely used as pathological or pharmacological models. However, most conventional microtissue construction strategies can only engineer simply predefined microcapsules with monotonous biological components in two dimensions. Here, we propose a flexible 3D microtissue construction method through self-bonding of real-time shape-programmable microcapsules. The microcapsules are prepared by photo-induced electrodeposition of cell-laden alginate hydrogel and flexibly tailored into tissue-specific shapes, sizes, and arbitrary biocomponents. With the local fluidics-guided assembly, the microcapsules are spatially organized into 3D perfectly aligned microtissues. To mimic in vivo intercellular connection, the aligned microcapsules are precoated with fibroblasts to self-bond the adjacent layers into a robust assemblage through fibroblast-extracellular matrix interactions, which highly reproduces the tissue morphogenesis in natural organisms. As a typical complex tissue model, the 3D hepatic lobule was engineered utilizing HepG2 cells seeded into microcapsules with a fibroblast coating, and its biofunction including albumin and urea secretion was improved by nearly two-fold compared with cells seeded without a fibroblast coating. We anticipate that our method will be capable of regenerating more complex multicellular constructs with unprecedented possibilities for future tissue engineering applications.
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http://dx.doi.org/10.1021/acsami.9b05108DOI Listing
July 2019

Storage of Extracellular Vesicles in Human Milk, and MicroRNA Profiles in Human Milk Exosomes and Infant Formulas.

J Pediatr Gastroenterol Nutr 2019 08;69(2):235-238

Department of Nutrition & Health Sciences.

The objectives of this study were to lay the methodological groundwork for field studies of microRNA analysis in exosomes from small sample volumes of human milk, and assess exosome and microRNA content in infant formulas. When human milk was stored at 4°C for 4 weeks, the count of exosome-sized vesicles decreased progressively to 49% ± 13% of that in fresh milk. Exosomes were purified from 1 mL of fresh human milk and their microRNA content was assessed by microRNA-sequencing analysis and compared with that in infant formulas. We identified 221 microRNAs in exosomes from 3 samples of fresh human milk; 84 microRNAs were present in all 3 samples. MicroRNAs were not detectable in infant formulas and their exosome-sized vesicles, which appeared to be casein micelles. We conclude that large-scale studies of microRNAs in human milk exosomes are feasible, and exosomes and microRNAs are not detectable in formulas.
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http://dx.doi.org/10.1097/MPG.0000000000002363DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6658346PMC
August 2019

Magnetic Micromachine Using Nickel Nanoparticles for Propelling and Releasing in Indirect Assembly of Cell-Laden Micromodules.

Micromachines (Basel) 2019 Jun 1;10(6). Epub 2019 Jun 1.

Intelligent Robotics Institute, School of Mechatronical Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China.

Magnetic micromachines as wireless end-effectors have been widely applied for drug discovery and regenerative medicine. Yet, the magnetic assembly of arbitrarily shaped cellular microstructures with high efficiency and flexibility still remains a big challenge. Here, a novel clamp-shape micromachine using magnetic nanoparticles was developed for the indirect untethered bioassembly. With a multi-layer template, the nickel nanoparticles were mixed with polydimethylsiloxane (PDMS) for mold replication of the micromachine with a high-resolution and permeability. To actuate the micromachine with a high flexibility and large scalable operation range, a multi-pole electromagnetic system was set up to generate a three-dimensional magnetic field in a large workspace. Through designing a series of flexible translations and rotations with a velocity of 15mm/s and 3 Hz, the micromachine realized the propel-and-throw strategy to overcome the inevitable adhesion during bioassembly. The hydrogel microstructures loaded with different types of cells or the bioactive materials were effectively assembled into microtissues with reconfigurable shape and composition. The results indicate that indirect magnetic manipulation can perform an efficient and versatile bioassembly of cellular micromodules, which is promising for drug trials and modular tissue engineering.
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http://dx.doi.org/10.3390/mi10060370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631220PMC
June 2019

Multicellular Co-Culture in Three-Dimensional Gelatin Methacryloyl Hydrogels for Liver Tissue Engineering.

Molecules 2019 May 7;24(9). Epub 2019 May 7.

Intelligent Robotics Institute, School of Mechatronical Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, China.

Three-dimensional (3D) tissue models replicating liver architectures and functions are increasingly being needed for regenerative medicine. However, traditional studies are focused on establishing 2D environments for hepatocytes culture since it is challenging to recreate biodegradable 3D tissue-like architecture at a micro scale by using hydrogels. In this paper, we utilized a gelatin methacryloyl (GelMA) hydrogel as a matrix to construct 3D lobule-like microtissues for co-culture of hepatocytes and fibroblasts. GelMA hydrogel with high cytocompatibility and high structural fidelity was determined to fabricate hepatocytes encapsulated micromodules with central radial-type hole by photo-crosslinking through a digital micromirror device (DMD)-based microfluidic channel. The cellular micromodules were assembled through non-contact pick-up strategy relying on local fluid-based micromanipulation. Then the assembled micromodules were coated with fibroblast-laden GelMA, subsequently irradiated by ultraviolet for integration of the 3D lobule-like microtissues encapsulating multiple cell types. With long-term co-culture, the 3D lobule-like microtissues encapsulating hepatocytes and fibroblasts maintained over 90% cell viability. The liver function of albumin secretion was enhanced for the co-cultured 3D microtissues compared to the 3D microtissues encapsulating only hepatocytes. Experimental results demonstrated that 3D lobule-like microtissues fabricated by GelMA hydrogels capable of multicellular co-culture with high cell viability and liver function, which have huge potential for liver tissue engineering and regenerative medicine applications.
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http://dx.doi.org/10.3390/molecules24091762DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6539120PMC
May 2019

High-dose Methotrexate plus temozolomide with or without rituximab in patients with untreated primary central nervous system lymphoma: A retrospective study from China.

Cancer Med 2019 04 1;8(4):1359-1367. Epub 2019 Mar 1.

Department of Medical Oncology, Sun Yat-Sen University Cancer Center, Guangzhou, China.

The purpose of this retrospective study was to compare the efficacy and toxicity of high-dose methotrexate plus temozolomide (MT regimen) and rituximab plus MT (RMT regimen) in patients with untreated primary central nervous system lymphoma (PCNSL). A total of 62 patients with untreated PCNSL were enrolled between January 2005 and December 2015, with the median age of 53.5 years (range 29-77).In this study, 32 patients received RMT as induction therapy, and 30 received MT. Objective responses were noted in 93.7% of the patients in the RMT group and in 69.0% of the patients in the MT group (P = 0.018), while complete responses were noted in 53.2% of the patients in the RMT group and 27.6% of the patients in the MT group (P < 0.001). The 2- and 5-year PFS rates were 81.3% and 53.3%, respectively, for the RMT group and 46.5% and 29.1%, respectively, for the MT group (P = 0.019). The 2- and 5-year overall survival (OS) rates were 82.3% and 82.3%, respectively, for the RMT group and 65.7% and 50.0%, respectively, for the MT group (P = 0.015). Multivariate analyses showed that therapeutic regimen (RMT vs MT) was an independent prognostic factor for PFS and OS. Our encouraging results suggest that the RMT regimen may be a feasible and safe therapeutic approach for first-line treatment of PCNSL.
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http://dx.doi.org/10.1002/cam4.1906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6488123PMC
April 2019

Fabrication of perfusable 3D hepatic lobule-like constructs through assembly of multiple cell type laden hydrogel microstructures.

Biofabrication 2018 12 27;11(1):015016. Epub 2018 Dec 27.

Intelligent Robotics Institute, School of Mechatronical Engineering, Beijing Institute of Technology, 5 South Zhongguancun Street, Haidian District, Beijing 100081, People's Republic of China.

The in vitro reproduction of three-dimensional (3D) cellular constructs to physiologically mimic human liver is highly desired for drug screening and clinical research. However, the fabrication of a liver-mimetic 3D model using traditional bottom-up technologies is challenging owing to the complex architecture and specific functions of real liver tissue. This work proposes a versatile strategy for spatially assembling gear-like microstructures encapsulating multiple cell types, and reorganizing them into 3D lobule-like micro-architecture with physiological relevance to native liver tissue. Gear-like microstructures were fabricated by photo-crosslinking poly(ethylene glycol) diacrylate (PEGDA) hydrogel mixed with hepatocytes and fibroblasts, in a digital micromirror device (DMD)-based microfluidic channel. The microstructures were assembled through coordinated micromanipulation based on local fluid force, and spatially self-aligned through hydrophilic-hydrophobic interactions into a 3D integrated construct with lobule-like morphology and a perfusable central lumen. The resulting 3D lobule-like constructs allowed long-term co-culture of hepatocytes and fibroblasts with high cell viability. The co-cultured constructs enhanced hepatocyte proliferation and spreading, as well as liver functions including a 50% increase in albumin secretion and urea synthesis. For hepatotoxicity assessment, the 3D lobule-like construct enabled drug perfusion through its built-in lumen for simulation of drug diffusion in the liver, which could improve the response sensitivity and efficiency to hepatotoxic drug. These results demonstrated that this method provides a valuable 3D co-culture model with perfusable lobule-like architecture and physiological functions, which has potential applications in drug discovery and tissue engineering applications.
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http://dx.doi.org/10.1088/1758-5090/aaf3c9DOI Listing
December 2018

Concentrations of Purine Metabolites Are Elevated in Fluids from Adults and Infants and in Livers from Mice Fed Diets Depleted of Bovine Milk Exosomes and their RNA Cargos.

J Nutr 2018 12;148(12):1886-1894

Departments of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE.

Background: Humans and mice absorb bovine milk exosomes and their RNA cargos.

Objectives: The objectives of this study were to determine whether milk exosome- and RNA-depleted (ERD) and exosome- and RNA-sufficient (ERS) diets alter the concentrations of purine metabolites in mouse livers, and to determine whether diets depleted of bovine milk alter the plasma concentration and urine excretion of purine metabolites in adults and infants, respectively.

Methods: C57BL/6 mice were fed ERD (providing 2% of the microRNA cargos compared with ERS) and ERS diets starting at age 3 wk; livers were collected at age 7 wk. Plasma and 24-h urine samples were collected from healthy adults who consumed (DCs) or avoided (DAs) dairy products. Spot urine samples were collected from healthy infants fed human milk (HM), milk formula (MF), or soy formula (SF) at age 3 mo. Purine metabolites were analyzed in liver, plasma, and urine; mRNAs and microRNAs were analyzed in the livers of female mice.

Results: We found that 9 hepatic purine metabolites in ERD-fed mice were 1.76 ± 0.43 times the concentrations in ERS-fed mice (P < 0.05). Plasma concentrations and urine excretion of purine metabolites in DAs was ≤1.62 ± 0.45 times the concentrations in DCs (P < 0.05). The excretion of 13 purine metabolites in urine from SF infants was ≤175 ± 39 times the excretion in HM and MF infants (P < 0.05). mRNA expression of 5'-nucleotidase, cytosolic IIIB, and adenosine deaminase in mice fed ERD was 0.64 ± 0.52 and 0.60 ± 0.28 times the expression in mice fed ERS, respectively.

Conclusion: Diets depleted of bovine-milk exosomes and RNA cargos caused increases in hepatic purine metabolites in mice, and in plasma and urine from human adults and infants, compared with exosome-sufficient controls. These findings are important, because purines play a role in intermediary metabolism and cell signaling.
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http://dx.doi.org/10.1093/jn/nxy223DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6280001PMC
December 2018

A Mobile-Based Diet Monitoring System for Obesity Management.

J Health Med Inform 2018 6;9(2). Epub 2018 Apr 6.

Department of Computer Science at McGill University, Canada.

Personal diet management is key to fighting the obesity epidemic. Recent advances in smartphones and wearable sensor technologies have empowered automated food monitoring through food image processing and eating episode detection, with the goal to conquer drawbacks of traditional food journaling that is labour intensive, inaccurate, and low adherent. In this paper, we present a new interactive mobile system that enables automated food recognition and assessment based on user food images and provides dietary intervention while tracking users' dietary and physical activities. In addition to using techniques in computer vision and machine learning, one unique feature of this system is the realization of real-time energy balance monitoring through metabolic network simulation. As a proof of concept, we have demonstrated the use of this system through an Android application.
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http://dx.doi.org/10.4172/2157-7420.1000307DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226023PMC
April 2018

Astrocyte EV-Induced lincRNA-Cox2 Regulates Microglial Phagocytosis: Implications for Morphine-Mediated Neurodegeneration.

Mol Ther Nucleic Acids 2018 Dec 29;13:450-463. Epub 2018 Sep 29.

Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68198-5880, USA. Electronic address:

Impairment of microglial functions, such as phagocytosis and/or dysregulation of immune responses, has been implicated as an underlying factor involved in the pathogenesis of various neurodegenerative disorders. Our previous studies have demonstrated that long intergenic noncoding RNA (lincRNA)-Cox2 expression is influenced by nuclear factor κB (NF-κB) signaling and serves as a coactivator of transcriptional factors to regulate the expression of a vast array of immune-related genes in microglia. Extracellular vesicles (EVs) have been recognized as primary facilitators of cell-to-cell communication and cellular regulation. Herein, we show that EVs derived from astrocytes exposed to morphine can be taken up by microglial endosomes, leading, in turn, to activation of Toll-like receptor 7 (TLR7) with a subsequent upregulation of lincRNA-Cox2 expression, ultimately resulting in impaired microglial phagocytosis. This was further validated in vivo, wherein inhibition of microglial phagocytic activity was also observed in brain slices isolated from morphine-administrated mice compared with control mice. Additionally, we also showed that intranasal delivery of EVs containing lincRNA-Cox2 siRNA (small interfering RNA) was able to restore microglial phagocytic activity in mice administered morphine. These findings have ramifications for the development of EV-loaded RNA-based therapeutics for the treatment of various disorders involving functional impairment of microglia.
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http://dx.doi.org/10.1016/j.omtn.2018.09.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202788PMC
December 2018

Comparative Transcriptome Analysis of Megacopta cribraria (Hemiptera: Plataspidae) in Response to High-Temperature Stress.

J Econ Entomol 2019 02;112(1):407-415

College of Agriculture, Jilin Agricultural University, Changchun, PR China.

Kudzu bug, Megacopta cribraria (Fabricius), is mainly distributed in southern China and has been considered an invasive species in the southeastern United States. Megacopta cribraria is a soybean pest with high-temperature resistance, but the molecular mechanisms underlying its thermal adaptation are largely unknown. Here, we performed comparative transcriptome analysis to unravel the molecular response of M. cribraria toward high-temperature stress. Following RNA-seq, we identified 93,959 assembled unigenes, 14,073 of which were annotated in M. cribraria transcriptome libraries. In addition, 127 differentially expressed unigenes (DEGs) were detected, 88 of them were significantly upregulated, whereas the remaining 39 genes were significantly downregulated. Functional classification revealed that the pathways of metabolic process, cellular processes, and single-organism processes were considered to be significantly enriched. In the COG classification, DEGs were mainly localized into O: post-translational modification, protein turnover, chaperone. Moreover, protein processing in endoplasmic reticulum and linoleic acid metabolism were significantly enriched among the 38 KEGG pathways. Further gene annotation analysis indicated that nine heat shock protein-related genes were significantly upregulated. Finally, five HSP DEGs were selected for real-time quantitative polymerase chain reaction validation and demonstrated a similar upregulation trend with RNA-seq expression profiles. Taken altogether, these findings provide new insights into the molecular mechanisms of thermal adaptation in M. cribraria.
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http://dx.doi.org/10.1093/jee/toy330DOI Listing
February 2019

Analysis of genetic and nongenetic factors influencing triglycerides-lowering drug effects based on paired observations.

BMC Proc 2018 17;12(Suppl 9):46. Epub 2018 Sep 17.

1Department of Statistics, University of Nebraska, 340 Hardin Hall North Wing, Lincoln, NE 68588 USA.

Obesity is a risk factor for heart disease, stroke, diabetes, high blood pressure, and other chronic diseases. Some drugs, including fenofibrate, are used to treat obesity or excessive weight by lowering the level of specific triglycerides. However, different groups have different drug sensitivities and, consequently, there are differences in drug effects. In this study, we assessed both genetic and nongenetic factors that influence drug responses and stratified patients into groups based on differential drug effect and sensitivity. Our methodology of investigating genetic factors and nongenetic factors is applicable to studying differential effects of other drugs, such as statins, and provides an approach to the development of personalized medicine.
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http://dx.doi.org/10.1186/s12919-018-0153-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157156PMC
September 2018

Genome-scale MicroRNA target prediction through clustering with Dirichlet process mixture model.

BMC Genomics 2018 Sep 24;19(Suppl 7):658. Epub 2018 Sep 24.

Systems Biology and Biomedical Informatics (SBBI) Laboratory, Department of Computer Science and Engineering, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.

Background: MicroRNA regulation is fundamentally responsible for fine-tuning the whole gene network in human and has been implicated in most physiological and pathological conditions. Studying regulatory impact of microRNA on various cellular and disease processes has resulted in numerous computational tools that investigate microRNA-mRNA interactions through the prediction of static binding site highly dependent on sequence pairing. However, what hindered the practical use of such target prediction is the interplay between competing and cooperative microRNA binding that complicates the whole regulatory process exceptionally.

Results: We developed a new method for improved microRNA target prediction based on Dirichlet Process Gaussian Mixture Model (DPGMM) using a large collection of molecular features associated with microRNA, mRNA, and the interaction sites. Multiple validations based on microRNA-mRNA interactions reported in recent large-scale sequencing analyses and a screening test on the entire human transcriptome show that our model outperformed several state-of-the-art tools in terms of promising predictive power on binding sites specific to transcript isoforms with reduced false positive prediction. Last, we illustrated the use of predicted targets in constructing conditional microRNA-mediated gene regulation networks in human cancer.

Conclusion: The probability-based binding site prediction provides not only a useful tool for differentiating microRNA targets according to the estimated binding potential but also a capability highly important for exploring dynamic regulation where binding competition is involved.
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http://dx.doi.org/10.1186/s12864-018-5029-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157162PMC
September 2018

Reply to B Fromm et al.

J Nutr 2018 09;148(9):1508

Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE.

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http://dx.doi.org/10.1093/jn/nxy145DOI Listing
September 2018

A diet defined by its content of bovine milk exosomes and their RNA cargos has moderate effects on gene expression, amino acid profiles and grip strength in skeletal muscle in C57BL/6 mice.

J Nutr Biochem 2018 09 12;59:123-128. Epub 2018 Jun 12.

Department of Nutrition & Health Sciences, University of Nebraska-Lincoln, 110 Leverton Hall, 1700 North 35(th) Street, Lincoln, NE 68583-0806, USA. Electronic address:

Exosomes are nanoparticles that transfer cargos from donor cells to recipient cells where they elicit changes in gene expression and metabolism. Evidence suggests that exosomes and their cargos are also absorbed from dietary sources such as bovine milk, and bovine exosomes promote the growth of myofibers in murine C2C12 myotube cell cultures. The aim of the current study was to determine whether the dietary intake of bovine milk exosomes alters strength, gene expression and amino acid profiles in murine skeletal muscles. Male and female C57BL/6 mice, age three weeks, were fed an AIN93G-based, exosome and RNA-depleted (ERD) diet for six weeks; controls were fed an exosome and RNA-sufficient (ERS) diet. Variables of feeding behavior, metabolism, grip strength, liver and kidney function, amino acid profiles, and gene expression patterns were analyzed by using metabolic cages, grip strength analyzers, clinical chemistry analyzers, targeted LC/MS-MS, and RNA sequencing analysis. The diets had no effect on food and water intake, respiratory exchange rate, physical activity, grip strength, markers of liver and kidney dysfunction, and amino acid profiles in muscle. Only twelve and nine mRNAs were differentially expressed in skeletal muscle from female and male mice, respectively, fed ERD and ERS diets. The modest effect of the ERD diet on gene expression and levels of free amino acids in skeletal muscle is consistent with observations that bovine milk exosomes and their cargos accumulate in tissues other than skeletal muscle.
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http://dx.doi.org/10.1016/j.jnutbio.2018.06.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6129415PMC
September 2018

Effect of Temperature on the Development, Survival, and Fecundity of Heliothis viriplaca (Lepidoptera: Noctuidae).

J Econ Entomol 2018 08;111(4):1940-1946

College of Agriculture, Jilin Agricultural University, Changchun, PR China.

Heliothis viriplaca (Hüfnagel) (Lepidoptera: Noctuidae) is worldwide an important economic pest of major agricultural crops. The effect of temperature on the biology, in particular the duration of the different developmental stages, of this insect pest was examined. The development, survival, and fecundity of H. viriplaca at five different temperatures (22, 25, 28, 31, and 34°C) were investigated, and experimental population life tables calculated. The results show that the developmental time of larvae, pupae, pre-oviposition, adults, and the entire generation decreases with increasing temperature. Larvae emerged fastest from the eggs at 28°C. The percentage of larvae that reached the seventh instar stage was 32.69, 38.60, 19.67, 3.61, and 20.88% at 22, 25, 28, 31, and 34°C, respectively. The developmental threshold temperature of the eggs, larvae, pupae, pre-oviposition and total pre-oviposition period was 10.53, 12.34, 5.60, 11.47, and 10.01°C, respectively. The effective accumulative temperature was 42.90, 239.68, 309.19, 77.72, and 767.64 degree-days, respectively. The population trend index (I) and the intrinsic rate of increase (r) of H. viriplaca were the highest at 31°C (133.97 and 0.12 d-1, respectively). The results of this study may be useful for predicting the population dynamics of H. viriplaca and thus provides information on this important insect pest that may assist in its management.
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http://dx.doi.org/10.1093/jee/toy151DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6074835PMC
August 2018