Publications by authors named "Juan Carlos Zuniga-Pflucker"

100Publications

A 2020 View of Thymus Stromal Cells in T Cell Development.

J Immunol 2021 Jan;206(2):249-256

Sunnybrook Research Institute, Toronto, Ontario M4N 3M5, Canada; and.

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January 2021

E2A regulates neural ectoderm fate specification in human embryonic stem cells.

Development 2020 12 13;147(23). Epub 2020 Dec 13.

Department of Cell Biology, School of Basic Medical Sciences, Peking University Stem Cell Research Center, Peking University, Beijing 100191, China

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December 2020

Wendy Havran: Scientist, mentor, advocate.

Immunol Rev 2020 Nov 16;298(1):289-291. Epub 2020 Oct 16.

Department of Biological Sciences, California State University San Marcos, San Marcos, CA, USA.

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November 2020

Thymic Engraftment by -Derived Progenitor T Cells in Young and Aged Mice.

Front Immunol 2020 18;11:1850. Epub 2020 Aug 18.

Department of Immunology, University of Toronto, Toronto, ON, Canada.

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August 2020

RBPJ-dependent Notch signaling initiates the T cell program in a subset of thymus-seeding progenitors.

Nat Immunol 2019 11 21;20(11):1456-1468. Epub 2019 Oct 21.

Sunnybrook Research Institute, Toronto, Ontario, Canada.

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November 2019

Notch and the pre-TCR coordinate thymocyte proliferation by induction of the SCF subunits Fbxl1 and Fbxl12.

Nat Immunol 2019 10 26;20(10):1381-1392. Epub 2019 Aug 26.

Section on Hematopoiesis and Lymphocyte Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.

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October 2019

Close Quarters Can Be a Good Fit for Stem Cells to Become T Cells.

Cell Stem Cell 2019 03;24(3):345-347

Department of Immunology, University of Toronto and Sunnybrook Research Institute, Toronto, ON M4N 3M5, Canada. Electronic address:

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March 2019

Producing proT cells to promote immunotherapies.

Int Immunol 2018 11;30(12):541-550

Department of Immunology, University of Toronto, Sunnybrook Research Institute, Toronto, Ontario, Canada.

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November 2018

Integration of T-cell receptor, Notch and cytokine signals programs mouse γδ T-cell effector differentiation.

Immunol Cell Biol 2018 10 28;96(9):994-1007. Epub 2018 Jun 28.

Department of Immunology, University of Toronto, and Sunnybrook Research Institute, 2075 Bayview Ave., Toronto, ON, M4N 3M5, Canada.

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October 2018

Genetic engineering in primary human B cells with CRISPR-Cas9 ribonucleoproteins.

J Immunol Methods 2018 06 31;457:33-40. Epub 2018 Mar 31.

Cardiovascular Research Institute, Sandler Asthma Basic Research Center, 555 Mission Bay Blvd S, University of California, San Francisco, San Francisco, CA 94143, USA; Department of Anatomy, 555 Mission Bay Blvd S, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address:

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June 2018

Role of a selecting ligand in shaping the murine γδ-TCR repertoire.

Proc Natl Acad Sci U S A 2018 02 5;115(8):1889-1894. Epub 2018 Feb 5.

Blood Cell Development and Function Program, Fox Chase Cancer Center, Philadelphia, PA 19111;

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February 2018

Control of HIV Infection In Vivo Using Gene Therapy with a Secreted Entry Inhibitor.

Mol Ther Nucleic Acids 2017 Dec 21;9:132-144. Epub 2017 Sep 21.

Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 3E2, Canada; Department of Laboratory Medicine & Pathobiology, University of Toronto, Toronto, ON, Canada. Electronic address:

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December 2017

Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.

Stem Cell Reports 2017 09 10;9(3):779-795. Epub 2017 Aug 10.

Department of Immunology, University of Toronto, Sunnybrook Research Institute, 2075 Bayview Avenue, Toronto, ON M4N 3M5, Canada. Electronic address:

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September 2017

Notch Shapes the Innate Immunophenotype in Breast Cancer.

Cancer Discov 2017 11 8;7(11):1320-1335. Epub 2017 Aug 8.

Campbell Family Institute for Cancer Research, Ontario Cancer Institute, Toronto, Ontario, Canada.

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November 2017

Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence.

Nat Commun 2017 05 25;8:15380. Epub 2017 May 25.

Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada M5S 3ES.

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May 2017

Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1.

Nat Methods 2017 May 10;14(5):531-538. Epub 2017 Apr 10.

Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada.

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May 2017

T Cell Genesis: In Vitro Veritas Est?

Trends Immunol 2016 12 24;37(12):889-901. Epub 2016 Oct 24.

Department of Immunology, University of Toronto, Sunnybrook Research Institute, 2075 Bayview Avenue, Toronto, ON M4 N 3M5, Canada. Electronic address:

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December 2016

Induction of T Cell Development In Vitro by Delta-Like (Dll)-Expressing Stromal Cells.

Methods Mol Biol 2016 ;1323:159-67

Department of Immunology, Sunnybrook Research Institute, University of Toronto, 2075 Bayview Avenue, Toronto, ON, Canada, M4N 3M5,

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May 2016

In Vitro T-Cell Generation From Adult, Embryonic, and Induced Pluripotent Stem Cells: Many Roads to One Destination.

Stem Cells 2015 Nov 11;33(11):3174-80. Epub 2015 Aug 11.

Division of Pediatric Blood and Marrow Transplant, University of Minnesota, Minneapolis, Minnesota, USA.

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November 2015

T cell development runs marrow deep.

J Exp Med 2015 May;212(5):599-600

University of Toronto and Sunnybrook Research Institute.

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May 2015

Hematopoiesis: from start to immune reconstitution potential.

Stem Cell Res Ther 2015 Apr 11;6:52. Epub 2015 Apr 11.

Department of Immunology, University of Toronto, 1 King's College Circle, Toronto, ON, M5S 1A8, Canada.

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April 2015

Gamma delta T-cell differentiation and effector function programming, TCR signal strength, when and how much?

Cell Immunol 2015 Jul 25;296(1):70-5. Epub 2015 Mar 25.

Department of Immunology, University of Toronto, and Sunnybrook Research Institute, Toronto, Ontario M4N 3M5, Canada. Electronic address:

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July 2015

Noncanonical mode of ERK action controls alternative αβ and γδ T cell lineage fates.

Immunity 2014 Dec 28;41(6):934-46. Epub 2014 Nov 28.

Blood Cell Development and Cancer Keystone, Immune Cell Development and Host Defense Program, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111-2497, USA. Electronic address:

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December 2014

Dedicated mTEC progenitors stay true, even into adulthood.

Immunity 2014 Nov 20;41(5):675-6. Epub 2014 Nov 20.

Department of Immunology, University of Toronto and Sunnybrook Research Institute, Toronto, ON M4N 3M5, Canada. Electronic address:

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November 2014

Derivation of T cells in vitro from mouse embryonic stem cells.

J Vis Exp 2014 Oct 14(92):e52119. Epub 2014 Oct 14.

Department of Biological Sciences, Hunter College and Graduate Center, City University of New York;

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October 2014

FOXN1 (GFP/w) reporter hESCs enable identification of integrin-β4, HLA-DR, and EpCAM as markers of human PSC-derived FOXN1(+) thymic epithelial progenitors.

Stem Cell Reports 2014 Jun 22;2(6):925-37. Epub 2014 May 22.

Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC 3800, Australia ; Murdoch Childrens Research Institute, The Royal Children's Hospital, Parkville, VIC 3052, Australia ; Department of Paediatrics, University of Melbourne, Parkville, VIC 3050, Australia.

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June 2014

An overview of the intrathymic intricacies of T cell development.

J Immunol 2014 May;192(9):4017-23

Anthony Nolan Research Institute, Royal Free Hospital, London NW3 2QG, United Kingdom.

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May 2014

Enforcement of γδ-lineage commitment by the pre-T-cell receptor in precursors with weak γδ-TCR signals.

Proc Natl Acad Sci U S A 2014 Apr 31;111(15):5658-63. Epub 2014 Mar 31.

Department of Immunology, Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada M4N 3M5.

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April 2014

Adapting in vitro embryonic stem cell differentiation to the study of locus control regions.

J Immunol Methods 2014 May 26;407:135-45. Epub 2014 Mar 26.

Department of Biological Sciences, City University of New York, Hunter College and Graduate Center, New York, NY 10065, United States. Electronic address:

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May 2014

Leukocyte infiltration and activation of the NLRP3 inflammasome in white adipose tissue following thermal injury.

Crit Care Med 2014 Jun;42(6):1357-64

1Sunnybrook Research Institute, Toronto, ON, Canada. 2Department of Immunology, University of Toronto, ON, Canada. 3Division of Plastic Surgery, Department of Surgery, University of Toronto, ON, Canada. 4Ross Tilley Burn Centre, Sunnybrook Health Sciences Centre, Toronto, ON, Canada.

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June 2014

The TCR ligand-inducible expression of CD73 marks γδ lineage commitment and a metastable intermediate in effector specification.

J Exp Med 2014 Feb 3;211(2):329-43. Epub 2014 Feb 3.

Blood Cell Development and Cancer Keystone, Immune Cell Development and Host Defense Program, Fox Chase Cancer Center, Philadelphia, PA 19111.

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February 2014

The orphan nuclear receptor Ear-2 (Nr2f6) is a novel negative regulator of T cell development.

Exp Hematol 2014 Jan 2;42(1):46-58. Epub 2013 Oct 2.

Department of Medical Biophysics, University of Toronto, Toronto, Canada; Biological Sciences, Sunnybrook Research Institute, Toronto, Canada; Department of Medicine, University of Toronto, Toronto, Canada; Department of Medical Oncology, Myelodysplastic Syndromes Program, Toronto Sunnybrook Regional Cancer Centre, Toronto, Canada. Electronic address:

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January 2014

Induction of T-cell development by Delta-like 4-expressing fibroblasts.

Int Immunol 2013 Oct 29;25(10):601-11. Epub 2013 Aug 29.

Department of Immunology, Sunnybrook Research Institute, University of Toronto, Toronto, Ontario, M4N 3M5 Canada.

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October 2013

Removal of myeloid cytokines from the cellular environment enhances T-cell development in vitro.

Int Immunol 2013 Oct 29;25(10):589-99. Epub 2013 Aug 29.

Haematology and Leukaemia Unit, St Vincent's Institute of Medical Research, 9 Princes Street, Fitzroy, Victoria 3065, Australia.

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October 2013

GATA-3 regulates the self-renewal of long-term hematopoietic stem cells.

Nat Immunol 2013 Oct 25;14(10):1037-44. Epub 2013 Aug 25.

1] Ontario Cancer Institute, University Health Network, Toronto, Canada. [2] Department of Medical Biophysics, University of Toronto, Toronto, Canada.

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October 2013

Directed differentiation of embryonic stem cells to the T-lymphocyte lineage.

Methods Mol Biol 2013 ;1029:119-28

Department of Immunology, Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada.

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March 2014

Complete TCR-α gene locus control region activity in T cells derived in vitro from embryonic stem cells.

J Immunol 2013 Jul 29;191(1):472-9. Epub 2013 May 29.

Department of Biological Sciences, City University of New York, Hunter College, New York, NY 10065, USA.

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July 2013

Generation, isolation, and engraftment of in vitro-derived human T cell progenitors.

Methods Mol Biol 2013 ;946:103-13

Department of Immunology, University of Toronto, Toronto, ON, Canada.

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May 2013

Role of recycling, Mindbomb1 association, and exclusion from lipid rafts of δ-like 4 for effective Notch signaling to drive T cell development.

J Immunol 2012 Dec 16;189(12):5797-808. Epub 2012 Nov 16.

Department of Immunology, University of Toronto and Sunnybrook Research Institute, Toronto, Ontario M4N 3M5, Canada.

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December 2012

Comparative and functional evaluation of in vitro generated to ex vivo CD8 T cells.

J Immunol 2012 Oct 27;189(7):3411-20. Epub 2012 Aug 27.

Department of Immunology, University of Toronto, Toronto, Ontario M4N 3M5, Canada.

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October 2012

Notch receptor-ligand interactions during T cell development, a ligand endocytosis-driven mechanism.

Curr Top Microbiol Immunol 2012 ;360:19-46

Department of Immunology, Sunnybrook Research Institute, University of Toronto, 2075 Bayview Avenue, Toronto, ON, M4 N 3M5, Canada.

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January 2013

Transcriptional priming of intrathymic precursors for dendritic cell development.

Development 2012 Jan;139(2):373-84

Sunnybrook Research Institute, Division of Biological Sciences, Toronto, Ontario, M4N 3M5, Canada.

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January 2012

On becoming a T cell, a convergence of factors kick it up a Notch along the way.

Semin Immunol 2011 Oct 6;23(5):350-9. Epub 2011 Oct 6.

Department of Immunology, University of Toronto, Sunnybrook Research Institute, Toronto, Ontario M4N 3M5, Canada.

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October 2011

Thymus-bound: the many features of T cell progenitors.

Front Biosci (Schol Ed) 2011 Jun 1;3:961-9. Epub 2011 Jun 1.

Department of Immunology, University of Toronto, Toronto, Ontario, Canada.

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June 2011

Human CD8 T cells generated in vitro from hematopoietic stem cells are functionally mature.

BMC Immunol 2011 Mar 23;12:22. Epub 2011 Mar 23.

Department of Immunology, University of Toronto, and Sunnybrook Research Institute, 2075 Bayview Avenue, Toronto, Ontario M4N 3M5, Canada.

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March 2011

A human thymic epithelial cell culture system for the promotion of lymphopoiesis from hematopoietic stem cells.

Exp Hematol 2011 May 4;39(5):570-9. Epub 2011 Feb 4.

Department of Microbiology and Immunology Stritch School of Medicine, Loyola University Chicago, 2160 First Avenue, Maywood, IL 60153, USA.

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May 2011

A Notch ligand, Delta-like 1 functions as an adhesion molecule for mast cells.

J Immunol 2010 Oct 1;185(7):3905-12. Epub 2010 Sep 1.

Division of Immunology, Department of Molecular and Cellular Biology, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Japan.

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October 2010

Determining γδ versus αß T cell development.

Nat Rev Immunol 2010 Sep 20;10(9):657-63. Epub 2010 Aug 20.

Molecular Pathogenesis Program, The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, New York 10016, USA.

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September 2010

Key players for T-cell regeneration.

Curr Opin Hematol 2010 Jul;17(4):327-32

Department of Immunology, University of Toronto, and Sunnybrook Research Institute, Toronto, Ontario, Canada.

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July 2010

Positive selection of T cells, an in vitro view.

Semin Immunol 2010 Oct;22(5):276-86

Department of Immunology, University of Toronto and Sunnybrook Research Institute, Toronto, Ontario M4N 3M5, Canada.

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October 2010

gammadelta and alphabeta T cell lineage choice: resolution by a stronger sense of being.

Semin Immunol 2010 Aug 13;22(4):228-36. Epub 2010 May 13.

Sunnybrook Research Institute, and Department of Immunology, University of Toronto, 2075 Bayview Avenue, Toronto, ON M4N3M5, Canada.

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August 2010

The OP9-DL1 system: generation of T-lymphocytes from embryonic or hematopoietic stem cells in vitro.

Cold Spring Harb Protoc 2009 Feb;2009(2):pdb.prot5156

Sunnybrook Research Institute and Department of Immunology, University of Toronto, Toronto, Ontario M4N 3M5, Canada.

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February 2009

Beyond tumor necrosis factor receptor: TRADD signaling in toll-like receptors.

Proc Natl Acad Sci U S A 2008 Aug 21;105(34):12429-34. Epub 2008 Aug 21.

The Campbell Family Institute for Breast Cancer Research, University Health Network and Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada M5G 2C1.

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August 2008

TLR Ligand-Induced Type I IFNs Affect Thymopoiesis.

J Immunol 2008 Jun;180(11):7134-46

Laboratoire d'Immunologie, Immunologie Humaine, Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Hôpital Saint-Luc, Montréal, Québec, Canada.

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June 2008

In vitro human T cell development directed by notch-ligand interactions.

Methods Mol Biol 2008 ;430:135-42

Department of Immunology, University of Toronto,Sunnybrook Research Institute, Toronto, Ontario, Canada.

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April 2008

Generation of pro-T cells in vitro: potential for immune reconstitution.

Semin Immunol 2007 Oct 9;19(5):341-9. Epub 2007 Nov 9.

Department of Immunology, University of Toronto, 2075 Bayview Avenue, Toronto, Ontario M4N 3M5, Canada.

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October 2007

Constitutive Notch signalling promotes CD4 CD8 thymocyte differentiation in the absence of the pre-TCR complex, by mimicking pre-TCR signals.

Int Immunol 2007 Dec 1;19(12):1421-30. Epub 2007 Nov 1.

Division of Cancer Sciences and Molecular Pathology, Section of Experimental Haematology, Royal Infirmary, University of Glasgow, Glasgow, UK.

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December 2007

Generation of immunocompetent T cells from embryonic stem cells.

Methods Mol Biol 2007 ;380:73-81

Department of Immunology, University of Toronto, Sunnybrook Research Institute, Toronto, Canada.

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April 2008

Commitment and developmental potential of extrathymic and intrathymic T cell precursors: plenty to choose from.

Immunity 2007 Jun;26(6):678-89

Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.

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June 2007

The thymus as an inductive site for T lymphopoiesis.

Annu Rev Cell Dev Biol 2007 ;23:463-93

Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016, USA.

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December 2007

T-cell potential and development in vitro: the OP9-DL1 approach.

Curr Opin Immunol 2007 Apr 15;19(2):163-8. Epub 2007 Feb 15.

Department of Immunology, University of Toronto, Sunnybrook Research Institute, 2075 Bayview Avenue, Toronto, ON M4N 3M5, Canada.

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April 2007

Zoned out: functional mapping of stromal signaling microenvironments in the thymus.

Annu Rev Immunol 2007 ;25:649-79

Scripps Florida Research Institute, Jupiter, Florida 33458, USA.

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June 2007